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ANALISIS KANDUNGAN KLOROFIL PADA DAUN DAN KANTONG Nepenthes gracilis [Analysis of Chlorophyll in Lamina and Pitcher of Nepenthes gracilis] Albar, Rahmat; Novitasari, Yuliana Diyah; Berlinda Paradisa, Yashanti; M, Des; Hilda Putri, Dwi; Chatri, Moralita Chatri; Solichatun, Solichatun; Wahyuni, Wahyuni
Berita Biologi Vol 22 No 3 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.966

Abstract

Pitcher plants are plant species that have unique shapes. Nepenthes spp. in the feature has become an important issue about his developments in research on carnivorous plants and rare plants. Nepenthes spp. has been classified as a protected plant due to excessive exploitation, which can damage the place of origin. The research in this article focuses on analyzing the content of chlorophyll a, b, and carotenoid in leaves and pitchers of Nepenthes gracilis. Leaves and pitchers (fresh and dried) were extracted in 3 types of solvents (acetone, methanol, and DMSO). The samples were then analyzed with a UV-VIS spectrophotometer. The results showed that the leaf and pitcher samples had quite different results. The leaf sample showed higher results than the pitcher sample, which had a chlorophyll a (3.434a), chlorophyll b (1.311a), carotenoids (0.918a), and total chlorophyll (4.745a) content. The pitcher sample had a content of chlorophyll a (1.463b), b (0.642b), carotenoids (0.445b), and total chlorophyll (2.105b). The treatment of samples given various solvents such as DMSO, Acetone, and Ethanol showed insignificant results on the content of chlorophyll a, chlorophyll b, carotenoids, and total chlorophyll. The conclusion is that all solvents can be used to analyze pigment content. Keywords: Carnivorous plant, Nepenthes gracilis, Pigment Content, Extracted solvents
Hydroponic Green Vegetable Cultivation Assistance in Support of SD Negeri 12 Padang Panjang’s Program Towards Achieving Adiwiyata School Violita, Violita; Holinesti, Rahmi; Sukma, Dina; Mukhlis, Mukhlis; Fronica, Imelda; Putri, Dwi Hilda; Achyar, Afifatul; Handayani, Dezi; Chatri, Moralita; Advinda, Linda; Farma, Siska Alicia; Nabilah, Rezi
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/291

Abstract

Abstract – SD Negeri 12 Padang Panjang is one of the schools that has made efforts to implement the Adiwiyata program. In 2024, the school represented the city of Padang Panjang in the Adiwiyata competition at the West Sumatra provincial level; however, it did not achieve optimal results. For the 2025 Adiwiyata program, SD Negeri 12 has set a target to become the provincial representative of West Sumatra. Based on the evaluation results from 2024, it was found that the assessment indicator for Participatory-Based Environmental Activities needs improvement. One activity that can support this effort is hydroponic cultivation. A hydroponic cultivation training was conducted by 26 fifth-grade students. The training included both theoretical material and hands-on practice in cultivating green vegetables using a hydroponic system. The training was guided by two experts who are competent in the field. This training successfully increased students' knowledge of hydroponic vegetable cultivation. Moreover, over 90% of the participants demonstrated teamwork and developed a greater awareness of environmental care. This outcome is a significant contribution to supporting SD Negeri 12 Padang Panjang Barat’s efforts toward achieving Adiwiyata School status.
Empowering Tourism Awareness Groups (Pokdarwis) through Biodiversity Education and Photography Training to Promote Conservation-Based Ecotourism in Singgalang Tandikek Nature Park Atifah, Yusni; Putri, Dwi Hilda; Achyar, Afifatul; Chatri, Moralita; Violita, Violita; Pratama, Sandi Fransisco; Vauzia, Vauzia; Putri, Irma Leilani; Putri, Aulia Devani
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/298

Abstract

At the present time, pressures on local ecosystems put many endemic species at risk of extinction if conservation measures are not promptly implemented. This community service program aimed to empower tourism awareness groups (Pokdarwis) in Singgalang Tandikek Nature Park through biodiversity education and photography training to support conservation-based ecotourism. The program targeted local guides, providing knowledge on endemic and protected species, particularly the Sumatran striped rabbit (Nesolagus netscheri), combined with practical photography skills using smartphones and ethical documentation practices. Activities included biodiversity lectures, hands-on photography, species identification, and social media promotion of local biodiversity. Evaluation using pre-test and post-tests showed an increase in participants’ photography knowledge (mean score 7.57 to 8.71), indicating improved awareness and skills in documenting biodiversity. The program successfully enhanced local capacity to promote sustainable ecotourism while fostering conservation ethics.
Application of Pseudomonas Berfluoresen PfCasCas3-molase on Bananas: Adaptation Measures by the Dahlia Flower Farmer Group in National Food Security and Climate Change Advinda, Linda; Putri, Dwi Hilda; Amelia, Kiki; Farma, Siska Alicia; Fevria, Resti; Putri, Santi Diana; Sari, Wilna
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/300

Abstract

Nagari Pematang Panjang, located in Sijunjung Regency, is expected to become the center of banana production in Sijunjung Regency. BDB disease is one of the problems in banana cultivation in the Bunga Dahlia Farmer Group in Sijunjung. Until now, BDB has been treated with chemical pesticides or, in more severe cases, banana plants are usually destroyed. To overcome this BDB disease, the UNP community service team provided understanding through presentations on the dangers of using chemical pesticides to members of the Bunga Dahlia Farmer Group and provided the Pseudomonas fluorescens PfCasCas3-molase formula as a biocontrol agent. The presentation of materials was effective in increasing the understanding of farmer group members from 25.98% to 81.27%. The technological innovation in the form of the PfCasCas3-molase formula was successfully disseminated to the community
Optimization of Manganese Peroxidase Enzyme Production from Pleurotus ostreatus Mushroom in Organik Medium Afroza, Faiza; Putri, Dwi Hilda; Irdawati, Irdawati; Farma, Siska Alicia; Andriani, Ade; Saskiawan, Iwan
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.8892

Abstract

The production of manganase peroxidase (MnP, a key lignolityc enzyme with broad applications in bioremediation and industrial processing, can be significantly enhanced trough the optimation of medium composition and fermentation time. This study aimed to determine the optimal organic medium, concentration, and incubation period for MnP production by Pleurotus ostreatus. Various plant-base substrates such as brown rice, mungbean, almond, red bean soybean, and potato dextrose broth (PDB) were evaluated using a descriptive method, withvthe obtained data calculated and analyzed in Excel, and the results presented in the form of diagrams. The results showed that brown rice medium is the most optimal medium to support the produvction of MnP (981,6 U/L), attribute to its high carbohydrate and micronutrien content. Futher optimization revealed that a consentration of 7,5%, while fermentation time optimization indicated that the 9th day was optima, producing up to 3207,1 U/L of enzyme activity. These findings suggest that selecting the right substrate and fermentation conditions can substantially improve MnP synthesis by P. ostreatus, making it a promising strategy for sustainable enzyme production and future biotecnological application. For future studies, it is recommended to explore large scale fermentation systems and alternative agro industrial waste susbtrates to further enhance enzyme yield and cost efficiency
PENGARUH RADIASI SMARTPHONE DALAM KONDISI AIRPLANE MODE TERHADAP HISTOLOGI GINJAL MENCIT BETINA Maulia Indah Ayuningtyas; Mellani Rachma; Fhuji Winardi; Echa Azkia Afandi; Yusni Atifah; Dwi Hilda Putri
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6697

Abstract

The rapid advancement of technology and information has led to a significant increase in smartphone usage in Indonesia, reaching 67.88% in 2022. Smartphones emit non-ionizing electromagnetic waves that may pose biological effects, particularly on the kidneys, which play a crucial role in maintaining the body's homeostasis. Previous studies have reported that electromagnetic radiation can induce histological damage in the kidneys through oxidative stress mechanisms, including tubular narrowing, Bowman’s capsule dilation, and tubular epithelial necrosis. Airplane mode is known to significantly reduce electromagnetic radiation exposure. This study aimed to determine the effect of smartphone radiation in airplane mode on the kidney histology of female mice. An experimental design was employed using four female mice divided into one control group and three treatment groups, which were exposed to a smartphone in airplane mode for 4, 6, and 8 hours per day for 7 consecutive days. Kidney tissues were processed using paraffin embedding and stained with Hematoxylin–Eosin for microscopic examination. The results showed no significant histological differences between the control and treatment groups. The glomerulus, Bowman’s capsule space, and proximal and distal convoluted tubules appeared normal without signs of narrowing, dilation, hemorrhage, or necrosis. These findings indicate that smartphone radiation in airplane mode does not cause histological damage to the kidneys of female mice.
The Development of Biology Module Based on Emotional Spiritual Quotient in Evolution Topic for Senior High School Fatma Rahmadhani; Armen Armen; Rahmawati Darussyamsu; Muhyiatul Fadilah; Dwi Hilda Putri
Scientiae Educatia: Jurnal Pendidikan Sains Vol. 8 No. 2 (2019): December 2019
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v8i2.2525

Abstract

This study aims to produce a valid biology module based on emotional spiritual quotient (ESQ) in an evolution topic for senior high school students grade XII. This conducted research used the Plomp development method. In this model, there were three steps established, such as; preliminary research, developing or prototype phase, and assessment phase. For implementation validity, the research subjects were a biology teacher and five biology lecturers. The instrumentation used were; interview questionnaire, self-evaluation sheet, and validity questionnaire. The obtained data analyzed by qualitative and quantitative methods. From the research, yield a new biology module with ESQ based in evolution lesson for senior high school students grade XII. Based on the expert review, obtained the score of validity 3.71 which indicated very valid criteria.
PENGEMBANGAN VIDEO PEMBELAJARAN BERBASIS SPARKOL VIDEOSCRIBE TERHADAP PESERTA DIDIK KELAS XI MAN 1 PASAMAN BARAT Monhartini Monhartini; Abdul Razak; Irdawati Irdawati; Dwi Hilda Putri
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3123

Abstract

This research aims to produce learning videos on circulation system material and the digestive system in humans that are valid, practical, and effective. The type of research used is development research using the Plomp development model. The development stage of this model starts from the initial investigation stage, the development or prototyping stage, and the assessment stage. The instruments used in this development research were teacher interview guideline instrument sheets, student response questionnaire sheets, self-evaluation sheets, and learning video validation sheets by experts, as well as learning video practicality assessment sheets filled out by teachers and students. The results of developing learning videos obtained very valid products with a value of 88.71%. The practicality of teachers and students is very practical with scores of 93.3 and 89.75%. The effectiveness test of this learning video is effective because based on the T-test conducted, there is a significant difference between the experimental class and the control class, namely the significance of 0.026, which means that the use of learning videos has an effect on improving student learning outcomes.
Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production Irdawati Irdawati; Fahra Fahra; Dwi Hilda Putri; Dezi Handayani; Yusrizal Yusrizal
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.3597

Abstract

Biofuels are fuels derived from biomass and consist of biogas, biodiesel and bioethanol. Bioethanol is a biofuel whose main ingredients are from plants and generally use microorganisms in the fermentation process. One way is to use thermophilic bacteria with the advantage of low contamination levels and better product quality. Bacteria that exist in nature are not only in a single form but also exist in the form of a consortium in which there are beneficial or detrimental bacterial interactions. Favorable bacterial interactions indicate that the bacteria are compatible. Using a consortium with compatible bacteria gives better results than using a single bacteria. This study aims to determine the compatibility and effect of a consortium of thermophilic bacteria from Mudiak Sapan hot springs on biofuel yields. This research is a descriptive research. To test the cooperation between isolates, a compatibility test was carried out using the disk diffusion method. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%.
Pengembangan Multimedia Interaktif Pembelajaran IPA-Biologi Menggunakan Macromedia Flash 8 untuk Peserta Didik Kelas VIII SMPN 34 Padang Tiffany Mantoviana; Linda Advinda; Moralita Chatri; Dwi Hilda Putri
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.5028

Abstract

The purpose of this research is to produce interactive multimedia using Macromedia Flash 8 for class VIII students at SMPN 34 Padang with material on the respiratory system and human excretory system that is valid, practical and effective. The data collection instruments used were interactive multimedia validation sheets by 3 validator lecturers, interactive multimedia practicality questionnaires by science teachers and students, as well as observation sheets on cognitive, affective, psychomotor aspects and learning activities. The results of the validation assessment by the validator of interactive multimedia on material on the human respiratory system and excretory system showed an average value of 82.33% (very valid). The results of the practicality assessment by science teachers showed an average score of 85.51% (very practical) and the results of the practicality assessment by students showed an average score of 91.64% (very practical). The results of the effectiveness test of interactive multimedia using Macromedia Flash 8 from the cognitive, affective, psychomotor and student activity aspects show that interactive multimedia using Macromedia Flash 8 is very effective. Thus, it can be concluded that the interactive multimedia using Macromedia Flash 8 developed is very valid, very practical and very effective.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul ADE ANDRIANI AERMA HASTUTY Afifatul Achyar Afionita, Santi Afroza, Faiza Ahmad Wibisana, Ahmad Albar, Rahmat Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Az-Zahra, Fauziah Azwir Anhar Berlinda Paradisa, Yashanti Cania Dewi, Rahmawitra Chatri, Moralita Chatri Cindy Pramila Darussyamsu, Rahmawati Des M Dezi Handayani Dezi Handayani Dina Sukma dina vaniana Dinda Sahara Djoelvinanda, Habibullah Echa Azkia Afandi Edwin Edwin Efliani Efliani Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fahra Fahra Fatma Rahmadhani Febri Doni Febrina Anggiastanti Feby Yeriska Fevria, Resti Fhuji Winardi Fhuji Winardi Fira Safitri Fira Safitri Fransisco, Sandi Fronica, Imelda Ghiffari, Muhammad Gustina Indriati Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal IWAN SASKIAWAN Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Kiki Amelia, Kiki Larasati Arum Utami Linda Advinda Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Marissa Angelina Maulia Indah Ayuningtyas Mellani Rachma Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhlis Mukhlis Mukhtar Mukhtar Mulia Mulia Mulia mutia anika Mutia Anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Ndaru Andri Damayanti Nisa Afifah Novitasari, Yuliana Diyah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nurfadillatun Nisa Wijaya Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Pratama, Sandi Fransisco Prima, Rika Putri Rachma Auliya Putri, Aulia Devani Putri, Cici Adelia Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resma Wahyuni Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Siska Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh SOLICHATUN SOLICHATUN Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Tiffany Mantoviana Tomi Apra Santosa Tomi Apra Santosa Santosa Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda Wahyuni Wahyuni wahyuni wahyuni Widya Ruchi Wilna Sari Wirda Taufik Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Zahara Nurfatihah Z Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri