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Peningkatan Kemampuan Guru MGMP MA Biologi Sumatera Barat dalam Mengimplementasikan Materi Bioteknologi Berbasis Bioentrepreneurship Handayani, Dezi; putri, dwi hilda; Atifah, Yusni; Eka Putri, Irma Leilani; Chatri, Moralita; Fransisco, Sandi
Pelita Eksakta Vol 7 No 1 (2024): Pelita Eksakta, Vol. 7, No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol7-iss1/226

Abstract

Terkait dengan taget Indonesia untuk mencapai kemandirian dibidang ekonomi pada tahun 2015-2085, maka penting usaha mendorong munculnya entreprenurship baru dari kalangan muda. Entrepreneurship dikalanganan anak muda usia sekola dapat dibelajarkan dengan menerapkan entrepreneurship pada mata pelajaran bioteknologi. Pengintegrasian muatan nilai-nilai kewirausahaan kedalam bidang ilmu hayati seperti bioteknologi dikenal dengan istilah bioentrepreneurship. Kendala yang dihadapi oleh guru biologi di MGMP MA Sumbar adalah masih rendah tentang aplikasi teknik produksi produk bioteknologi. Keterbatasan juga dimiliki guru mengenai potensi produk bioteknologi yang mengandalkan kekayaan alam Sumatera Barat. Permasalahan inilah yang menjadi dasar pelatihan pembuatan produk bioteknologi berbasis bioentreprenur. Kegiatan di hadiri oleh 40 guru yang tergabung dalam MGMP MA Biologi Sumatera Barat. Pelatihan menghadirkan 3 orang narasumber yang berkopeten. Kegiatan ini tentu diharapkan adanya peningkatakan kemampuan guru-guru MGMP dalam praktek metode bioteknologi untuk menumbuhkan jiwa bioentrepreneurship generasi muda.
Training by the PUPIK Team, FMIPA UNP Biology Laboratory, on Making Nata De Coco and Production Quality Testing During the Storage Process putri, dwi hilda; Advinda, Linda; Irdawati, Irdawati; Fevria, Resti; Chatri, Moralita; rahmi, elva; Ghiffari, Muhammad; Viona, Alda
Pelita Eksakta Vol 6 No 1 (2023): Pelita Eksakta Vol. 6 No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol6-iss1/252

Abstract

Nata de coco is one way to use coconut water waste that can no longer be used so that it can reduce environmental pollution. The quality of nata as a food and beverage product must be monitored. This article aims to analyze the shelf life of Nata de coco based on its shelf life. The work methods used include: 1) testing the durability of nata de coco at room temperature, and 2) testing the durability of nata de coco at cold temperatures or in the refrigerator. Organoleptic tests carried out included aroma, color, taste and texture. The results achieved show that nata de coco stored in the refrigerator can last four weeks, while those stored at room temperature can only last for one week
ANALISIS KANDUNGAN KLOROFIL PADA DAUN DAN KANTONG Nepenthes gracilis [Analysis of Chlorophyll in Lamina and Pitcher of Nepenthes gracilis] Albar, Rahmat; Novitasari, Yuliana Diyah; Berlinda Paradisa, Yashanti; M, Des; Hilda Putri, Dwi; Chatri, Moralita Chatri; Solichatun, Solichatun; Wahyuni, Wahyuni
Berita Biologi Vol 22 No 3 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.966

Abstract

Pitcher plants are plant species that have unique shapes. Nepenthes spp. in the feature has become an important issue about his developments in research on carnivorous plants and rare plants. Nepenthes spp. has been classified as a protected plant due to excessive exploitation, which can damage the place of origin. The research in this article focuses on analyzing the content of chlorophyll a, b, and carotenoid in leaves and pitchers of Nepenthes gracilis. Leaves and pitchers (fresh and dried) were extracted in 3 types of solvents (acetone, methanol, and DMSO). The samples were then analyzed with a UV-VIS spectrophotometer. The results showed that the leaf and pitcher samples had quite different results. The leaf sample showed higher results than the pitcher sample, which had a chlorophyll a (3.434a), chlorophyll b (1.311a), carotenoids (0.918a), and total chlorophyll (4.745a) content. The pitcher sample had a content of chlorophyll a (1.463b), b (0.642b), carotenoids (0.445b), and total chlorophyll (2.105b). The treatment of samples given various solvents such as DMSO, Acetone, and Ethanol showed insignificant results on the content of chlorophyll a, chlorophyll b, carotenoids, and total chlorophyll. The conclusion is that all solvents can be used to analyze pigment content. Keywords: Carnivorous plant, Nepenthes gracilis, Pigment Content, Extracted solvents
Hydroponic Green Vegetable Cultivation Assistance in Support of SD Negeri 12 Padang Panjang’s Program Towards Achieving Adiwiyata School Violita, Violita; Holinesti, Rahmi; Sukma, Dina; Mukhlis, Mukhlis; Fronica, Imelda; Putri, Dwi Hilda; Achyar, Afifatul; Handayani, Dezi; Chatri, Moralita; Advinda, Linda; Farma, Siska Alicia; Nabilah, Rezi
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/291

Abstract

Abstract – SD Negeri 12 Padang Panjang is one of the schools that has made efforts to implement the Adiwiyata program. In 2024, the school represented the city of Padang Panjang in the Adiwiyata competition at the West Sumatra provincial level; however, it did not achieve optimal results. For the 2025 Adiwiyata program, SD Negeri 12 has set a target to become the provincial representative of West Sumatra. Based on the evaluation results from 2024, it was found that the assessment indicator for Participatory-Based Environmental Activities needs improvement. One activity that can support this effort is hydroponic cultivation. A hydroponic cultivation training was conducted by 26 fifth-grade students. The training included both theoretical material and hands-on practice in cultivating green vegetables using a hydroponic system. The training was guided by two experts who are competent in the field. This training successfully increased students' knowledge of hydroponic vegetable cultivation. Moreover, over 90% of the participants demonstrated teamwork and developed a greater awareness of environmental care. This outcome is a significant contribution to supporting SD Negeri 12 Padang Panjang Barat’s efforts toward achieving Adiwiyata School status.
Empowering Tourism Awareness Groups (Pokdarwis) through Biodiversity Education and Photography Training to Promote Conservation-Based Ecotourism in Singgalang Tandikek Nature Park Atifah, Yusni; Putri, Dwi Hilda; Achyar, Afifatul; Chatri, Moralita; Violita, Violita; Pratama, Sandi Fransisco; Vauzia, Vauzia; Putri, Irma Leilani; Putri, Aulia Devani
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/298

Abstract

At the present time, pressures on local ecosystems put many endemic species at risk of extinction if conservation measures are not promptly implemented. This community service program aimed to empower tourism awareness groups (Pokdarwis) in Singgalang Tandikek Nature Park through biodiversity education and photography training to support conservation-based ecotourism. The program targeted local guides, providing knowledge on endemic and protected species, particularly the Sumatran striped rabbit (Nesolagus netscheri), combined with practical photography skills using smartphones and ethical documentation practices. Activities included biodiversity lectures, hands-on photography, species identification, and social media promotion of local biodiversity. Evaluation using pre-test and post-tests showed an increase in participants’ photography knowledge (mean score 7.57 to 8.71), indicating improved awareness and skills in documenting biodiversity. The program successfully enhanced local capacity to promote sustainable ecotourism while fostering conservation ethics.
Application of Pseudomonas Berfluoresen PfCasCas3-molase on Bananas: Adaptation Measures by the Dahlia Flower Farmer Group in National Food Security and Climate Change Advinda, Linda; Putri, Dwi Hilda; Amelia, Kiki; Farma, Siska Alicia; Fevria, Resti; Putri, Santi Diana; Sari, Wilna
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/300

Abstract

Nagari Pematang Panjang, located in Sijunjung Regency, is expected to become the center of banana production in Sijunjung Regency. BDB disease is one of the problems in banana cultivation in the Bunga Dahlia Farmer Group in Sijunjung. Until now, BDB has been treated with chemical pesticides or, in more severe cases, banana plants are usually destroyed. To overcome this BDB disease, the UNP community service team provided understanding through presentations on the dangers of using chemical pesticides to members of the Bunga Dahlia Farmer Group and provided the Pseudomonas fluorescens PfCasCas3-molase formula as a biocontrol agent. The presentation of materials was effective in increasing the understanding of farmer group members from 25.98% to 81.27%. The technological innovation in the form of the PfCasCas3-molase formula was successfully disseminated to the community
UJI AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT PSEUDOMONAS BERFLUORESEN TERHADAP Escherichia coli, Staphylococcus aureus DAN Candida Eka Nuraini, Fauzi; Hilda Putri, Dwi; Advinda, Linda; Irdawati; Handayani, Dezi; Mardhiyah Nazri, Laila; Wulandari, Tesya; Febrianti, Liza
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.7989

Abstract

Antimicrobial resistance is increasing every year. New and better antimicrobial sources are needed. One source of antimicrobials that can be explored is from endophytic bacteria. This study aims to test the ability of fluorescent Pseudomonas endophytic bacteria from the roots of plantain lemongrass in producing antimicrobial compounds. The type of research is descriptive. The antimicrobial activity of fluorescent Pseudomonas isolates was tested using the point inoculation method using test microbes E. coli, S. aureus and C. albicans. The clear zone was observed after 24 hours. The results showed that fluorescent Pseudomonas isolates were able to inhibit E. coli with a diameter of 3.9 mm found in isolate 51. S. aureus bacteria had an inhibition zone of 9.4 mm in isolate 89. Interesting results were shown by isolate 51 in inhibiting C. albicans with an inhibition zone diameter of 29.9 mm. These differences in response are thought to be related to variations in cell wall structure and the presence of an outer membrane in Gram-negative bacteria, which inhibits the penetration of active compounds. Overall, fluorescent Pseudomonas endophytic bacteria from the roots of the plantain have the potential to be developed as a source of new antifungal candidates against C. albicans, while their antibacterial activity still needs to be optimized through compound purification and further testing.
AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT (Pseudomonas Berfluoresen) DARI AKAR PISANG BUAI (Musa x paradisiaca L.) Astrid; Hilda Putri, Dwi; Handayani, Dezi
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8176

Abstract

Infectious diseases caused by pathogenic bacteria and fungi remain a global health problem, particularly due to the increasing incidence of antimicrobial resistance, highlighting the need for effective alternative antimicrobial sources. Endophytic bacteria, especially fluorescent Pseudomonas, are known to produce secondary metabolites with antibacterial and antifungal properties. This study aimed to evaluate the antimicrobial activity of endophytic fluorescent Pseudomonas isolated from the roots of banana buai (Musa × paradisiaca L.) and to identify isolates with the highest activity. This experimental study employed a Completely Randomized Design (CRD) using 44 isolates with three replications. Antimicrobial activity was tested in vitro using the spot inoculation method against Escherichia coli, Staphylococcus aureus, and Candida albicans. The observed parameter was the diameter of the inhibition zone, which was analyzed using analysis of variance (ANOVA) followed by Duncan’s Multiple Range Test. The results showed that antimicrobial activity varied among isolates and was specific to the test microorganisms. Isolate PFPB 32 exhibited the highest activity against E. coli and S. aureus, while isolate PFPB 300 showed the strongest antifungal activity against C. albicans. These findings indicate that endophytic fluorescent Pseudomonas from banana buai roots have potential as a source of antimicrobial agents, particularly antifungal agents against Candida albicans
Optimization of Manganese Peroxidase Enzyme Production from Pleurotus ostreatus Mushroom in Organik Medium Afroza, Faiza; Putri, Dwi Hilda; Irdawati, Irdawati; Farma, Siska Alicia; Andriani, Ade; Saskiawan, Iwan
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.8892

Abstract

The production of manganase peroxidase (MnP, a key lignolityc enzyme with broad applications in bioremediation and industrial processing, can be significantly enhanced trough the optimation of medium composition and fermentation time. This study aimed to determine the optimal organic medium, concentration, and incubation period for MnP production by Pleurotus ostreatus. Various plant-base substrates such as brown rice, mungbean, almond, red bean soybean, and potato dextrose broth (PDB) were evaluated using a descriptive method, withvthe obtained data calculated and analyzed in Excel, and the results presented in the form of diagrams. The results showed that brown rice medium is the most optimal medium to support the produvction of MnP (981,6 U/L), attribute to its high carbohydrate and micronutrien content. Futher optimization revealed that a consentration of 7,5%, while fermentation time optimization indicated that the 9th day was optima, producing up to 3207,1 U/L of enzyme activity. These findings suggest that selecting the right substrate and fermentation conditions can substantially improve MnP synthesis by P. ostreatus, making it a promising strategy for sustainable enzyme production and future biotecnological application. For future studies, it is recommended to explore large scale fermentation systems and alternative agro industrial waste susbtrates to further enhance enzyme yield and cost efficiency
LITERATUR REVIEW: POTENSI LIMBAH PERTANIAN DAN PERKEBUNAN DALAM PRODUKSI BIOETANOL GENERASI KEDUA DI INDONESIA: Indonesia Winardi, Fhuji; Putri, Dwi Hilda
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6253

Abstract

Permintaan energi global yang meningkat dan penipisan sumber daya fosil mendorong pencarian bahan bakar terbarukan, seperti bioetanol generasi kedua dari limbah lignoselulosa. Namun, produksinya menghadapi tantangan teknis, terutama pada pretreatment dan efisiensi konversi akibat kompleksitas struktur biomassa. Artikel review ini bertujuan menganalisis metode pemanfaatan limbah pertanian dan perkebunan Indonesia untuk produksi bioetanol generasi kedua, mengidentifikasi peluang dan tantangan, serta memberikan rekomendasi strategis. Metode yang digunakan adalah literature review terhadap artikel terpublikasi dalam lima tahun terakhir dari Google Scholar dan ScienceDirect dengan kata kunci terkait. Hasil review menunjukkan keragaman bahan baku seperti TKKS, ampas tebu, kulit buah dan dominasi pretreatment kimia serta hidrolisis asam untuk mendegradasi lignin. Fermentasi dengan Saccharomyces cerevisiae dengan konsentrasi dan waktu bervariasi menghasilkan variasi yield bioetanol, yield bioetanol tertinggi dari limbah kulit buah nanas dengan pretreatment intensif sebesar 59%. Disimpulkan bahwa keberhasilan produksi bergantung pada sinergi optimal pretreatment, hidrolisis, dan fermentasi, dengan kebutuhan inovasi pada metode ramah lingkungan dan optimasi proses.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul ADE ANDRIANI AERMA HASTUTY Afandi, Echa Azkia Afionita, Santi Afroza, Faiza Ahmad Wibisana, Ahmad Albar, Rahmat Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Andri Damayanti, Ndaru Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Astrid Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Ayuningtyas, Maulia Indah Az-Zahra, Fauziah Azwir Anhar Berlinda Paradisa, Yashanti Cania Dewi, Rahmawitra Chatri, Moralita Chatri Cindy Pramila Darussyamsu, Rahmawati Des M Dezi Handayani Dezi Handayani Dilla Yanthi, Nova Dina Sukma dina vaniana Dinda Sahara Djoelvinanda, Habibullah Dwi Adinda Putri, Desrinda Echa Azkia Afandi Edwin Edwin Efliani Efliani Eka Nuraini, Fauzi Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fahra Fahra Farras, Fadhila Annisa Fatma Rahmadhani Febri Doni Febrianti, Liza Febrina Anggiastanti Feby Yeriska Ferdyan, Rhavy Fevria, Resti Fhuji Winardi Fira Safitri Fitri Arsih Fransisco, Sandi Fronica, Imelda Ghiffari, Muhammad Gustina Indriati Hamdah, Alya Handayani , Dezi Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal IWAN SASKIAWAN Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Kiki Amelia, Kiki Larasati Arum Utami Linda Advinda Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Mardhiyah Nazri, Laila Marissa Angelina Mellani Rachma Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhlis Mukhlis Mukhtar Mukhtar Mulia Mulia Mulia mutia anika Mutia Anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Nazri, Laila Mardhiyah Nisa Afifah Novitasari, Yuliana Diyah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nuraini, Fauzi Eka Nurfadillatun Nisa Wijaya Nurfatihah Z, Zahara Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Pratama, Sandi Fransisco Prima, Rika Putri Rachma Auliya Putri, Aulia Devani Putri, Cici Adelia Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resma Wahyuni Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal safitri, fira Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Saputra, Sugiyono Sari, Rinti Mutiara Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh SOLICHATUN SOLICHATUN Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Tiffany Mantoviana Tomi Apra Santosa Tomi Apra Santosa Santosa Turnip, Natalia Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda Wahyuni Wahyuni wahyuni wahyuni Widya Ruchi Wilna Sari Winardi, Fhuji Wirda Taufik Wulandari, Tesya Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yunavita, Diva Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri