Article Per Year (5 Year)
p-Index From 2020 - 2025
9.372
P-Index
This Author published in this journals
All Journal Jurnal Biologi Udayana Bioscience Bioeducation Journal Jurnal Ilmu Dasar AL KAUNIYAH Jurnal Biotek Medisiana Indonesia Althea Medical Journal Biota Biomedika BIO-SITE |BIOLOGI Sains Terapan Jurnal Penelitian dan Pembelajaran IPA Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Jurnal Agrotek Tropika Bio-Lectura Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Ilmu Kefarmasian Indonesia AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Majalah Kesehatan Pharmamedika Pelita Eksakta Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi IJECA (International Journal of Education and Curriculum Application) Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Biosense Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Tropical Genetics Jurnal Serambi Biologi
Dwi Hilda Putri
Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

Published : 96 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 96 Documents
Search

 6   7   8   9   10 
Primer Design and Optimization of Annealing Temperature for Gene Amplification GSTL2 on Rice Violita, Violita; Achyar, Afifatul; Zulyusri, Zulyusri; Atifah, Yusni; Putri, Dwi Hilda; Nabilah, Rezi
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.32859

Abstract

AbstractGlutathione S-Transferase is a superfamily enzyme that has many roles for living things, especially in the detoxification of Reactive Oxygen Species (ROS), one of which is rice plants. One gene of this family that has a role for plants is GSTL2 This gene is known to have a contribution to Arabidopsis and Oryza sativa L. against abiotic stress. To find out how this gene expression is requires a primer to specifically amplify this gene, and an optimal annealing temperature to support the success of the primer. This study aims to design primers and determine the optimal annealing temperature for gene amplification GSTL2. Primers were designed using the Primer3, which were then analyzed with Genious Prime based on good primer criteria and optimizing the annealing temperature which was carried out using gradient PCR. The results of this study obtained a primer pair is forward 5’-TTCGAAGGGCCAGCATTACT-3’ primer reverse 5’-CAATGTCCACCAAGCTGAA-3’. The primer pair has a length of 20 nt, with melting temperature (Tm) 59–59,4 °C, and GC content 50%. The primer forward there is a secondary structure in the form of a self-dimer with (Tm) 6.2 °C. The primer pair can amplify gene sequences GSTL2 by producing a PCR product 224 bp. The annealing temperature of 60 °C resulting in a single thick, bright DNA strand.AbstrakGlutathione S-Transferase merupakan enzim superfamily yang memiliki banyak peranan bagi makhluk hidup terutama dalam detoksifikasi Reactive Oxygen Species (ROS), salah satunya tumbuhan. Salah satu gen dari famili ini yang memiliki peranan bagi tanaman adalah GSTL2. Gen ini diketahui memiliki kontribusi bagi tanaman Arabidopsis dan Oryza sativa L. terhadap stres abiotik. Untuk mengetahui bagaimana ekspresi gen GSTL2 ini dibutuhkan primer untuk mengamplifikasi gen ini secara spesifik, di samping itu suhu annealing yang optimal untuk menunjang keberhasilan primer. Penelitian ini bertujuan untuk mendesain primer dan menentukan suhu annealing yang optimal untuk mengamplifikasi gen GSTL2. Primer didesain menggunakan tools Pick Primer dan Geneious Prime, yang kemudian dianalisis secara in silico berdasarkan kriteria primer yang baik. Serta optimasi suhu annealing yang dilakukan menggunakan gradient PCR. Hasil penelitian ini diperoleh sepasang primer dengan panjang masing-masing primer 20 nt, primer forward 5’-TTCGAAGGGCCAGCATTACT-3’ primer reverse 5’-CAATGTCCACCAAGCTGAA-3’. Pasangan primer dapat mengamplifikasi sekuen gen GSTL2 dengan menghasilkan produk PCR sebesar 224 bp pada suhu annealing 60 °C.
Peningkatan Kemampuan Guru MGMP MA Biologi Sumatera Barat dalam Mengimplementasikan Materi Bioteknologi Berbasis Bioentrepreneurship Handayani, Dezi; putri, dwi hilda; Atifah, Yusni; Eka Putri, Irma Leilani; Chatri, Moralita; Fransisco, Sandi
Pelita Eksakta Vol 7 No 1 (2024): Pelita Eksakta, Vol. 7, No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol7-iss1/226

Abstract

Terkait dengan taget Indonesia untuk mencapai kemandirian dibidang ekonomi pada tahun 2015-2085, maka penting usaha mendorong munculnya entreprenurship baru dari kalangan muda. Entrepreneurship dikalanganan anak muda usia sekola dapat dibelajarkan dengan menerapkan entrepreneurship pada mata pelajaran bioteknologi. Pengintegrasian muatan nilai-nilai kewirausahaan kedalam bidang ilmu hayati seperti bioteknologi dikenal dengan istilah bioentrepreneurship. Kendala yang dihadapi oleh guru biologi di MGMP MA Sumbar adalah masih rendah tentang aplikasi teknik produksi produk bioteknologi. Keterbatasan juga dimiliki guru mengenai potensi produk bioteknologi yang mengandalkan kekayaan alam Sumatera Barat. Permasalahan inilah yang menjadi dasar pelatihan pembuatan produk bioteknologi berbasis bioentreprenur. Kegiatan di hadiri oleh 40 guru yang tergabung dalam MGMP MA Biologi Sumatera Barat. Pelatihan menghadirkan 3 orang narasumber yang berkopeten. Kegiatan ini tentu diharapkan adanya peningkatakan kemampuan guru-guru MGMP dalam praktek metode bioteknologi untuk menumbuhkan jiwa bioentrepreneurship generasi muda.
Training by the PUPIK Team, FMIPA UNP Biology Laboratory, on Making Nata De Coco and Production Quality Testing During the Storage Process putri, dwi hilda; Advinda, Linda; Irdawati, Irdawati; Fevria, Resti; Chatri, Moralita; rahmi, elva; Ghiffari, Muhammad; Viona, Alda
Pelita Eksakta Vol 6 No 1 (2023): Pelita Eksakta Vol. 6 No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol6-iss1/252

Abstract

Nata de coco is one way to use coconut water waste that can no longer be used so that it can reduce environmental pollution. The quality of nata as a food and beverage product must be monitored. This article aims to analyze the shelf life of Nata de coco based on its shelf life. The work methods used include: 1) testing the durability of nata de coco at room temperature, and 2) testing the durability of nata de coco at cold temperatures or in the refrigerator. Organoleptic tests carried out included aroma, color, taste and texture. The results achieved show that nata de coco stored in the refrigerator can last four weeks, while those stored at room temperature can only last for one week
PENGEMBANGAN VIDEO PEMBELAJARAN BERBASIS SPARKOL VIDEOSCRIBE TERHADAP PESERTA DIDIK KELAS XI MAN 1 PASAMAN BARAT Monhartini, Monhartini; Razak, Abdul; Irdawati, Irdawati; Putri, Dwi Hilda
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3123

Abstract

This research aims to produce learning videos on circulation system material and the digestive system in humans that are valid, practical, and effective. The type of research used is development research using the Plomp development model. The development stage of this model starts from the initial investigation stage, the development or prototyping stage, and the assessment stage. The instruments used in this development research were teacher interview guideline instrument sheets, student response questionnaire sheets, self-evaluation sheets, and learning video validation sheets by experts, as well as learning video practicality assessment sheets filled out by teachers and students. The results of developing learning videos obtained very valid products with a value of 88.71%. The practicality of teachers and students is very practical with scores of 93.3 and 89.75%. The effectiveness test of this learning video is effective because based on the T-test conducted, there is a significant difference between the experimental class and the control class, namely the significance of 0.026, which means that the use of learning videos has an effect on improving student learning outcomes.
Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production Irdawati, Irdawati; Fahra, Fahra; Putri, Dwi Hilda; Handayani, Dezi; Yusrizal, Yusrizal
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.3597

Abstract

Biofuels are fuels derived from biomass and consist of biogas, biodiesel and bioethanol. Bioethanol is a biofuel whose main ingredients are from plants and generally use microorganisms in the fermentation process. One way is to use thermophilic bacteria with the advantage of low contamination levels and better product quality. Bacteria that exist in nature are not only in a single form but also exist in the form of a consortium in which there are beneficial or detrimental bacterial interactions. Favorable bacterial interactions indicate that the bacteria are compatible. Using a consortium with compatible bacteria gives better results than using a single bacteria. This study aims to determine the compatibility and effect of a consortium of thermophilic bacteria from Mudiak Sapan hot springs on biofuel yields. This research is a descriptive research. To test the cooperation between isolates, a compatibility test was carried out using the disk diffusion method. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%.
Pengembangan Multimedia Interaktif Pembelajaran IPA-Biologi Menggunakan Macromedia Flash 8 untuk Peserta Didik Kelas VIII SMPN 34 Padang Mantoviana, Tiffany; Advinda, Linda; Chatri, Moralita; Putri, Dwi Hilda
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.5028

Abstract

The purpose of this research is to produce interactive multimedia using Macromedia Flash 8 for class VIII students at SMPN 34 Padang with material on the respiratory system and human excretory system that is valid, practical and effective. The data collection instruments used were interactive multimedia validation sheets by 3 validator lecturers, interactive multimedia practicality questionnaires by science teachers and students, as well as observation sheets on cognitive, affective, psychomotor aspects and learning activities. The results of the validation assessment by the validator of interactive multimedia on material on the human respiratory system and excretory system showed an average value of 82.33% (very valid). The results of the practicality assessment by science teachers showed an average score of 85.51% (very practical) and the results of the practicality assessment by students showed an average score of 91.64% (very practical). The results of the effectiveness test of interactive multimedia using Macromedia Flash 8 from the cognitive, affective, psychomotor and student activity aspects show that interactive multimedia using Macromedia Flash 8 is very effective. Thus, it can be concluded that the interactive multimedia using Macromedia Flash 8 developed is very valid, very practical and very effective.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul AERMA HASTUTY Afionita, Santi Ahmad Wibisana, Ahmad Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Andri Damayanti, Ndaru Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Az-Zahra, Fauziah Azwir Anhar Cania Dewi, Rahmawitra Cindy Pramila Darussyamsu, Rahmawati Dezi Handayani Dezi Handayani dina vaniana Dinda Sahara Djoelvinanda, Habibullah Edwin Edwin Efliani Efliani Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fahra Fahra Fahra, Fahra Fatma Rahmadhani Febri Doni Febrina Anggiastanti Feby Yeriska Fevria, Resti Fira Safitri Fransisco, Sandi Ghiffari, Muhammad Gustina Indriati Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Larasati Arum Utami Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Mantoviana, Tiffany Marissa Angelina Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini, Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhtar Mukhtar Mulia Mulia Mulia mutia anika Mutia Anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Nisa Afifah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nurfadillatun Nisa Wijaya Nurfatihah Z, Zahara Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Prima, Rika Putri Rachma Auliya Putri, Cici Adelia Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal safitri, fira Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Sari, Rinti Mutiara Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Tomi Apra Santosa Tomi Apra Santosa Santosa Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda Wahyuni Wahyuni Wahyuni, Resma Widya Ruchi Wirda Taufik Y Yusrizal Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri