Claim Missing Document
Check
Articles

Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production Irdawati Irdawati; Fahra Fahra; Dwi Hilda Putri; Dezi Handayani; Yusrizal Yusrizal
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.3597

Abstract

Biofuels are fuels derived from biomass and consist of biogas, biodiesel and bioethanol. Bioethanol is a biofuel whose main ingredients are from plants and generally use microorganisms in the fermentation process. One way is to use thermophilic bacteria with the advantage of low contamination levels and better product quality. Bacteria that exist in nature are not only in a single form but also exist in the form of a consortium in which there are beneficial or detrimental bacterial interactions. Favorable bacterial interactions indicate that the bacteria are compatible. Using a consortium with compatible bacteria gives better results than using a single bacteria. This study aims to determine the compatibility and effect of a consortium of thermophilic bacteria from Mudiak Sapan hot springs on biofuel yields. This research is a descriptive research. To test the cooperation between isolates, a compatibility test was carried out using the disk diffusion method. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%.
Comparison of Fenol-Kloroform Method and Mini-Prep CTAB Method for Chili (Capsicum annum L.) Plant DNA Isolation Sri Okta Handayani; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Chili (Capsicum annum L.) is the most popular horticultural commodity in Indonesia. This high level of interest must be accompanied by an increase in the quality and quantity of chili production. One way to increase the quality and quantity of chilies is through plant breeding. Genetic variation analysis is one of the success factors in plant breeding. DNA isolation is the first step in analyzing genetic variation. There are various methods of plant DNA isolation, but DNA isolation methods using the fenol-kloroform method and CTAB mini-prep have not been widely reported. In this study, a comparison of the DNA isolation method of chili plants was carried out with the least DNA acquisition and contamination. This study used accessions of Capsicum annum L. (Cibinong03). The results of the qualitative evaluation study on confirmation of DNA samples were that there were no DNA bands found in the 0.8% agaorasa gel electrophoresis results, this result was inversely proportional to the results of the CTAB mini-prep method which produced DNA band bands in chilli accessions.
POTENTIAL OF THERMOPHIC BACTERIAL XILANASE AS ENVIRONMENTALLY FRIENDLY FABRIC BLEACH Nur Shofiatun Nisa; IRDAWATI IRDAWATI; Dwi Hilda Putri; Dezi Handayani; Y Yusrizal
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The main conclusion contains what is the implication to other studies or to the future brought by the study. Xylanase is an enzyme that is widely used in industry because this enzyme can hydrolyze xylan (hemicellulose) into xylo-oligosaccharides and xylose. This enzyme can be produced by thermophilic bacteria. One of the benefits of xylanase is as an environmentally friendly fabric bleaching process (biobleaching). The use of xylanase enzymes can reduce the use of toxic chemicals such as chlorine by about 20-40%. The purpose of this study was to determine the effect of temperature and pH on xylanase production activity using rice straw xylan extract as a substrate and to see the potential of MS 18 thermophilic bacterial xylanase on fabric brightness. This research is a descriptive study with temperature and pH optimization treatment using 6 treatments and 3 replications, respectively. Enzyme activity was measured using a spectrophotometer with a wavelength of 540 nm. The data obtained are displayed in the form of tables and figures. The xylanase potential on the brightness level of the fabric was seen based on the results of the kappa number. The results of this study obtained the optimum temperature of xylanase activity at a temperature of 50℃ with the highest xylanase 3.792 U/mL. while at pH the optimum pH of xylanase activity was found at pH 7 with the highest xylanase production of 4.448 U/mL. The potential of xylanase on the brightness of the fabric based on the results of the kappa number is 2.04 lower than without using xylanase, which is 6.46.
Effect of Antimicrobial Activity 0f Starfruit Leaf Extract (Averrhoa bilimbi L.) on the Growth of Staphylococcus aureus Bacteria in Vitro Efliani Efliani; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to determine the effect of the antimicrobial activity of star fruit leaf extract on the growth of S.aureus bacteria. This study was conducted using the complete randomized design (RAL) method with 5 treatments and 3 repetitions. The treatment carried out is: the concentration of star fruit leaf extract is 10%, 20%, 30%, 40%, and 50%. The data obtained were analyzed using ANOVA with a confidence level of α= 0.05 and further tested with DNMRT. Antibacterial testing is carried out by the disc diffusion method to determine the formed inhibitory zone. The results showed the average diameter of the formation of the inhibitory zone at a concentration of 10% of 10.10 mm, 20% of 12.56 mm, 30% of 13.08 mm, 40% of 14.57 mm, and 50% of 15.97 mm. The optimum concentration of star fruit leaf extract in inhibiting the growth of S.aureus bacteria at a concentration of 50% is 15.97 mm. Key words: Staphylococcus aureus, antimicrobial, star fruit, inhibitory zone.
Test of Antimicrobial Activity of Rimbang Leaf (Solanum torvum) Ethanol Extract on Escherichia coli and Candida albicans Aura Iga Maharani; Rahmatul Huda Asra; Aulia Yunita; Resti Desmayanti; Husnul Khatimah; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Aims: One of the types of traditional medicinal plants is rimbang (Solanus frowned). Rimbang is often used as an alternative medicine by rural communities because it is useful as a medicine for coughs, wounds, fever, boils and scabs. Rimbang leaf extract is known to contain flavonoids and tannins (polyphenolic) which have antimicrobial properties. The purpose of this study was to prove rimbang leaf extract was able to inhibit growth E.coli and C.albicans. Methods: Rimbang leaf extraction was carried out by maceration for three days with 96% ethanol solvent. Then evaporate using rotaryevaporator until thick extract is formed. The antimicrobial activity test was carried out by the agar diffusion method using disc paper by measuring the diameter of the inhibition zone for bacterial growth. The test concentrations used varied from 50%, 25%, 12.5%, 6.25% and 3.125%. Result: The results showed that rimbang leaf extract had antimicrobial activity against E.coli and C.albicans Main Conclusion: The antimicrobial activity test with strong inhibition was found in E. coli with a concentration of 6.25% an inhibition zone diameter of 12.15 mm, moderate inhibition at a concentration of 3.125% with an inhibition zone diameter of 9.5 mm, and moderate inhibition on C. albicans concentration of 50% with an inhibition zone diameter
The Prediction of the Interaction Genistein and Daidzein Compounds on ESR2 Expression by Molecular Docking Quratul Akyuni; Dwi Hilda Putri; Yuni Ahda
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Aims: Estrogen is known to have various roles in the body. ESR2 is an estrogen receptor expressing estrogen receptor beta (ERβ). ERβ is expressed in normal breast epithelial cells as well as in various other tissues such as the uterus, ovary, prostate and brain, and breast cancer cells. One plant that is thought to have the potential to increase ERβ expression is soybean (Glycine max). Soybean plants contain very important phytochemicals, so they are widely used in the food industry as supplements and included in drug formulations. Methods: This type of research is descriptive research conducted using in silico method through the molecular docking approach. The metabolites used were genistein and daidzein for the target protein ESR 2 (PDB ID: 1QKM). Result: The docking results showed that the binding affinity value of the metabolites genistein, daidzein, and native ligand was -9.7 kcal/mol. The RMSD value obtained from LigRMSD was 0.40 with very similar visualization between the ligand test before and after docking. Main Conclusion: The conclusion of this study is that the metabolites of genistein and daidzein are predicted to have the ability to increase ESR2 expression in the human body.
The Effect of Micro Radiation on Microbial DNA Nurul Pratiwi; Cindy Pramila; Fira Safitri; Syifa Kamila Namidya; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Aim One of the important steps in molecular studies is DNA extraction. Many methods have been developed for bacterial DNA extraction, using either kits or boiling techniques. For the boiling technique, heating can be done using a water bath, heatblock, and microwave. Microwave is a tool that uses micro radiation rays. This study aims to determine the effect of microwave irradiation on bacterial DNA. Methods The isolates used in this study were B.J.T.A.2.1 isolates which were inoculated into NB medium. Microwave exposure was carried out for 0, 30, and 90 seconds. Cultures were isolated using the QIAamp DNA Mini Kit. The quality of DNA after exposure to the microwave was analyzed from the electrophoretic DNA bands of PCR RAPD products. Results Microwave exposure of bacteria causes changes to DNA. PCR RAPD reaction using isolated DNA from bacteria exposed to microwaves produces new bands on electrophoresis results. The longer the bacteria are exposed to the microwave, the brighter and thicker the new DNA banding pattern will be. Main conclusion Microwave exposure to bacterial culture affects the isolated DNA. The longer the culture is exposed to the microwave, the brighter and thicker the new DNA banding pattern will be.
Potential of Pseudomonad Fluoresen as Control of Plant Diseases Silviana Okwisan; Linda Advinda; Dezi Handayani; Dwi Hilda Putri; Irma Leilani Eka Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The use of fertilizers and pesticides provides various side effects such as environmental pollution and higher production costs due to the high prices of fertilizers and pesticides. Therefore it is necessary to look for other alternatives to control plant diseases. One of the safe and environmentally friendly control measures is to use biological control agents. Fluorescent pseudomonads are antagonistic bacteria that are widely used as biological agents for fungi, pathogenic bacteria and viruses. Biological control using fluorescent pseudomonads has been studied for its effectiveness in suppressing plant diseases. This study aims to collect and analyze articles related to the potential of fluorescent pseudomonads in controlling plant diseases. This type of research is research that uses the literature review method and collects literature using internet sources from the Google Scholar database. The stages consist of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that fluorescent pseudomonads are potential and effective in controlling various plant diseases.
Fluorescent Pseudomonad Association in Plant Rhizosphere Intan Febriani; Linda Advinda; Dezi Handayani; Siska Alicia Farma; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Plant growth promoting rhizobacteria or known as Plant Growth Promoting Rhizobacteria (PGPR) are bacteria found on the surface of plant roots and are beneficial microorganisms. The rhizosphere is an ideal area for the growth and development of soil microorganisms. This situation is supported by its function, namely as a provider of nutrients and also as a place for the growth and development of microorganisms. One of the bacteria found in the rhizosphere of plants is the fluorescent pseudomonad. Fluorescent pseudomonads are biological agents that can be isolated from the surface area of plant roots. This study aims to collect and analyze articles related to the association of fluorescent pseudomonads from plant rhizosphere. This type of research is research that uses the literature review method and literature collection using internet sources from the Google Scholar database. This stage consists of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that the use of fluorescent pseudomonads as a biological control agent has been widely carried out because of their ability to produce antimicrobial compounds.
Fungi Isolated from litter’s forest of Aka Barayun, Lembah Harau and It’s Phosphate Solubilizing Activity Nurul Rahmi; Dezi Handayani; Dwi Hilda Putri; Violita Violita
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One of the essential nutrients for plant growth is phosphate, but just 0.1-0.5% is available for plants to use. This problem can be solved by using chemical fertilizers, but for long run could damages environment. An alternative way for chemical fertilizers is using phosphate solubilizing fungi. Phosphate-solubilizing fungi can be isolated from soil or litter. This study aims to isolated fungi from litter’s forest of Aka Barayun, Lembah Harau, West Sumatra and determine it’s phosphate solubilizing potency. Medium for isolation was Potato Dextrose Agar (PDA) and medium for phosphate solubilizing activity test was solid Pikovskaya medium with source of bound phosphate Ca3(PO4)2. Thirteen fungi were isolated successfully from litter’s forest of Aka Barayun, Lembah Harau, West Sumatra, which are coded SR1-SR13. Based on macroscopic and microscopic morphology, it is known that two isolates belong to the genus Penicillium, two isolates belong to Trichoderma, one isolate of Verticillium, one isolate of Fusarium, two isolates of yeast and two isolates are not yet known. Results of the phosphate solubilizing test showed that only seven isolates were able to solubilize phosphate, they are isolates SR2, SR5, SR6, SR7, SR11, SR 12 and SR 13. Phosphate solubilization index is low and ranges from 0.1 to 0.4.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul ADE ANDRIANI AERMA HASTUTY Afandi, Echa Azkia Afionita, Santi Afroza, Faiza Ahmad Wibisana, Ahmad Albar, Rahmat Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Andri Damayanti, Ndaru Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Astrid Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Ayuningtyas, Maulia Indah Az-Zahra, Fauziah Azwir Anhar Berlinda Paradisa, Yashanti Cania Dewi, Rahmawitra Chatri, Moralita Chatri Cindy Pramila Darussyamsu, Rahmawati Des M Dezi Handayani Dezi Handayani Dilla Yanthi, Nova Dina Sukma dina vaniana Dinda Sahara Djoelvinanda, Habibullah Dwi Adinda Putri, Desrinda Echa Azkia Afandi Edwin Edwin Efliani Efliani Eka Nuraini, Fauzi Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fahra Fahra Farras, Fadhila Annisa Fatma Rahmadhani Febri Doni Febrianti, Liza Febrina Anggiastanti Feby Yeriska Ferdyan, Rhavy Fevria, Resti Fhuji Winardi Fira Safitri Fitri Arsih Fransisco, Sandi Fronica, Imelda Ghiffari, Muhammad Gustina Indriati Hamdah, Alya Handayani , Dezi Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal IWAN SASKIAWAN Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Kiki Amelia, Kiki Larasati Arum Utami Linda Advinda Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Mardhiyah Nazri, Laila Marissa Angelina Mellani Rachma Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhlis Mukhlis Mukhtar Mukhtar Mulia Mulia Mulia mutia anika Mutia Anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Nazri, Laila Mardhiyah Nisa Afifah Novitasari, Yuliana Diyah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nuraini, Fauzi Eka Nurfadillatun Nisa Wijaya Nurfatihah Z, Zahara Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Pratama, Sandi Fransisco Prima, Rika Putri Rachma Auliya Putri, Aulia Devani Putri, Cici Adelia Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resma Wahyuni Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal safitri, fira Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Saputra, Sugiyono Sari, Rinti Mutiara Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh SOLICHATUN SOLICHATUN Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Tiffany Mantoviana Tomi Apra Santosa Tomi Apra Santosa Santosa Turnip, Natalia Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda Wahyuni Wahyuni wahyuni wahyuni Widya Ruchi Wilna Sari Winardi, Fhuji Wirda Taufik Wulandari, Tesya Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yunavita, Diva Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri