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All Journal Jurnal Biologi Udayana Bioscience Bioeducation Journal Jurnal Ilmu Dasar AL KAUNIYAH Jurnal Biotek Medisiana Indonesia Althea Medical Journal Biota Biomedika BIO-SITE |BIOLOGI Sains Terapan Jurnal Penelitian dan Pembelajaran IPA Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Jurnal Agrotek Tropika Bio-Lectura Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Ilmu Kefarmasian Indonesia AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Majalah Kesehatan Pharmamedika Pelita Eksakta Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi IJECA (International Journal of Education and Curriculum Application) Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Biosense Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Tropical Genetics Jurnal Serambi Biologi Berita Biologi
Dwi Hilda Putri
Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Optimization of Specific PCR Conditions for Cows (Bos taurus) in Rendang Samples for Molecular-Based Halal Tests Nurfadillatun Nisa Wijaya; Sisca Alicia Farma; Dwi Hilda Putri; Afifatul Achyar
Tropical Genetics Vol. 2 No. 1 (2022):
Publisher : Genetikawan Muda Indonesia

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Abstract

Rendang is one of the popular foods in Indonesia made from beef as the main ingredient, which has great potential in tourism development. As a country that has a Muslim majority population, this is very influential for the halal tourism industry. Halal tourism is the parent of tourism in accordance with Islamic principles. The issue of halal food is a sensitive issue for the community. The rise of mixing of non-halal ingredients such as pork in processed food products has worried the public, especially for adherents of the Islamic religion. However, this can be detected molecularly using the Polymerase Chain Reaction (PCR) technique which will amplify the DNA of the target gene of the species to be identified. Each gene has a primer with a different concentration and annealing temperature, so that prior to PCR with research samples, it is necessary to optimize the primer concentration and annealing temperature in order to obtain the appropriate PCR composition and conditions so as to obtain optimal PCR results. In this study, optimization of bovine-specific BOS primers that amplify the ND5 gene in mitochondrial DNA was carried out, namely ND5 (NADH dehydrogenase subunit 5). The results showed that the optimum annealing temperature was 48.2ºC, the optimum primary concentration was 0.5 μM.
Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production Irdawati Irdawati; Fahra Fahra; Dwi Hilda Putri; Dezi Handayani; Yusrizal Yusrizal
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.3597

Abstract

Biofuels are fuels derived from biomass and consist of biogas, biodiesel and bioethanol. Bioethanol is a biofuel whose main ingredients are from plants and generally use microorganisms in the fermentation process. One way is to use thermophilic bacteria with the advantage of low contamination levels and better product quality. Bacteria that exist in nature are not only in a single form but also exist in the form of a consortium in which there are beneficial or detrimental bacterial interactions. Favorable bacterial interactions indicate that the bacteria are compatible. Using a consortium with compatible bacteria gives better results than using a single bacteria. This study aims to determine the compatibility and effect of a consortium of thermophilic bacteria from Mudiak Sapan hot springs on biofuel yields. This research is a descriptive research. To test the cooperation between isolates, a compatibility test was carried out using the disk diffusion method. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%.
Comparison of Fenol-Kloroform Method and Mini-Prep CTAB Method for Chili (Capsicum annum L.) Plant DNA Isolation Sri Okta Handayani; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Chili (Capsicum annum L.) is the most popular horticultural commodity in Indonesia. This high level of interest must be accompanied by an increase in the quality and quantity of chili production. One way to increase the quality and quantity of chilies is through plant breeding. Genetic variation analysis is one of the success factors in plant breeding. DNA isolation is the first step in analyzing genetic variation. There are various methods of plant DNA isolation, but DNA isolation methods using the fenol-kloroform method and CTAB mini-prep have not been widely reported. In this study, a comparison of the DNA isolation method of chili plants was carried out with the least DNA acquisition and contamination. This study used accessions of Capsicum annum L. (Cibinong03). The results of the qualitative evaluation study on confirmation of DNA samples were that there were no DNA bands found in the 0.8% agaorasa gel electrophoresis results, this result was inversely proportional to the results of the CTAB mini-prep method which produced DNA band bands in chilli accessions.
POTENTIAL OF THERMOPHIC BACTERIAL XILANASE AS ENVIRONMENTALLY FRIENDLY FABRIC BLEACH Nur Shofiatun Nisa; IRDAWATI IRDAWATI; Dwi Hilda Putri; Dezi Handayani; Y Yusrizal
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The main conclusion contains what is the implication to other studies or to the future brought by the study. Xylanase is an enzyme that is widely used in industry because this enzyme can hydrolyze xylan (hemicellulose) into xylo-oligosaccharides and xylose. This enzyme can be produced by thermophilic bacteria. One of the benefits of xylanase is as an environmentally friendly fabric bleaching process (biobleaching). The use of xylanase enzymes can reduce the use of toxic chemicals such as chlorine by about 20-40%. The purpose of this study was to determine the effect of temperature and pH on xylanase production activity using rice straw xylan extract as a substrate and to see the potential of MS 18 thermophilic bacterial xylanase on fabric brightness. This research is a descriptive study with temperature and pH optimization treatment using 6 treatments and 3 replications, respectively. Enzyme activity was measured using a spectrophotometer with a wavelength of 540 nm. The data obtained are displayed in the form of tables and figures. The xylanase potential on the brightness level of the fabric was seen based on the results of the kappa number. The results of this study obtained the optimum temperature of xylanase activity at a temperature of 50℃ with the highest xylanase 3.792 U/mL. while at pH the optimum pH of xylanase activity was found at pH 7 with the highest xylanase production of 4.448 U/mL. The potential of xylanase on the brightness of the fabric based on the results of the kappa number is 2.04 lower than without using xylanase, which is 6.46.
Effect of Antimicrobial Activity 0f Starfruit Leaf Extract (Averrhoa bilimbi L.) on the Growth of Staphylococcus aureus Bacteria in Vitro Efliani Efliani; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

This study aims to determine the effect of the antimicrobial activity of star fruit leaf extract on the growth of S.aureus bacteria. This study was conducted using the complete randomized design (RAL) method with 5 treatments and 3 repetitions. The treatment carried out is: the concentration of star fruit leaf extract is 10%, 20%, 30%, 40%, and 50%. The data obtained were analyzed using ANOVA with a confidence level of α= 0.05 and further tested with DNMRT. Antibacterial testing is carried out by the disc diffusion method to determine the formed inhibitory zone. The results showed the average diameter of the formation of the inhibitory zone at a concentration of 10% of 10.10 mm, 20% of 12.56 mm, 30% of 13.08 mm, 40% of 14.57 mm, and 50% of 15.97 mm. The optimum concentration of star fruit leaf extract in inhibiting the growth of S.aureus bacteria at a concentration of 50% is 15.97 mm. Key words: Staphylococcus aureus, antimicrobial, star fruit, inhibitory zone.
Test of Antimicrobial Activity of Rimbang Leaf (Solanum torvum) Ethanol Extract on Escherichia coli and Candida albicans Aura Iga Maharani; Rahmatul Huda Asra; Aulia Yunita; Resti Desmayanti; Husnul Khatimah; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Aims: One of the types of traditional medicinal plants is rimbang (Solanus frowned). Rimbang is often used as an alternative medicine by rural communities because it is useful as a medicine for coughs, wounds, fever, boils and scabs. Rimbang leaf extract is known to contain flavonoids and tannins (polyphenolic) which have antimicrobial properties. The purpose of this study was to prove rimbang leaf extract was able to inhibit growth E.coli and C.albicans. Methods: Rimbang leaf extraction was carried out by maceration for three days with 96% ethanol solvent. Then evaporate using rotaryevaporator until thick extract is formed. The antimicrobial activity test was carried out by the agar diffusion method using disc paper by measuring the diameter of the inhibition zone for bacterial growth. The test concentrations used varied from 50%, 25%, 12.5%, 6.25% and 3.125%. Result: The results showed that rimbang leaf extract had antimicrobial activity against E.coli and C.albicans Main Conclusion: The antimicrobial activity test with strong inhibition was found in E. coli with a concentration of 6.25% an inhibition zone diameter of 12.15 mm, moderate inhibition at a concentration of 3.125% with an inhibition zone diameter of 9.5 mm, and moderate inhibition on C. albicans concentration of 50% with an inhibition zone diameter
The Prediction of the Interaction Genistein and Daidzein Compounds on ESR2 Expression by Molecular Docking Quratul Akyuni; Dwi Hilda Putri; Yuni Ahda
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Aims: Estrogen is known to have various roles in the body. ESR2 is an estrogen receptor expressing estrogen receptor beta (ERβ). ERβ is expressed in normal breast epithelial cells as well as in various other tissues such as the uterus, ovary, prostate and brain, and breast cancer cells. One plant that is thought to have the potential to increase ERβ expression is soybean (Glycine max). Soybean plants contain very important phytochemicals, so they are widely used in the food industry as supplements and included in drug formulations. Methods: This type of research is descriptive research conducted using in silico method through the molecular docking approach. The metabolites used were genistein and daidzein for the target protein ESR 2 (PDB ID: 1QKM). Result: The docking results showed that the binding affinity value of the metabolites genistein, daidzein, and native ligand was -9.7 kcal/mol. The RMSD value obtained from LigRMSD was 0.40 with very similar visualization between the ligand test before and after docking. Main Conclusion: The conclusion of this study is that the metabolites of genistein and daidzein are predicted to have the ability to increase ESR2 expression in the human body.
The Effect of Micro Radiation on Microbial DNA Nurul Pratiwi; Cindy Pramila; Fira Safitri; Syifa Kamila Namidya; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Aim One of the important steps in molecular studies is DNA extraction. Many methods have been developed for bacterial DNA extraction, using either kits or boiling techniques. For the boiling technique, heating can be done using a water bath, heatblock, and microwave. Microwave is a tool that uses micro radiation rays. This study aims to determine the effect of microwave irradiation on bacterial DNA. Methods The isolates used in this study were B.J.T.A.2.1 isolates which were inoculated into NB medium. Microwave exposure was carried out for 0, 30, and 90 seconds. Cultures were isolated using the QIAamp DNA Mini Kit. The quality of DNA after exposure to the microwave was analyzed from the electrophoretic DNA bands of PCR RAPD products. Results Microwave exposure of bacteria causes changes to DNA. PCR RAPD reaction using isolated DNA from bacteria exposed to microwaves produces new bands on electrophoresis results. The longer the bacteria are exposed to the microwave, the brighter and thicker the new DNA banding pattern will be. Main conclusion Microwave exposure to bacterial culture affects the isolated DNA. The longer the culture is exposed to the microwave, the brighter and thicker the new DNA banding pattern will be.
Potential of Pseudomonad Fluoresen as Control of Plant Diseases Silviana Okwisan; Linda Advinda; Dezi Handayani; Dwi Hilda Putri; Irma Leilani Eka Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The use of fertilizers and pesticides provides various side effects such as environmental pollution and higher production costs due to the high prices of fertilizers and pesticides. Therefore it is necessary to look for other alternatives to control plant diseases. One of the safe and environmentally friendly control measures is to use biological control agents. Fluorescent pseudomonads are antagonistic bacteria that are widely used as biological agents for fungi, pathogenic bacteria and viruses. Biological control using fluorescent pseudomonads has been studied for its effectiveness in suppressing plant diseases. This study aims to collect and analyze articles related to the potential of fluorescent pseudomonads in controlling plant diseases. This type of research is research that uses the literature review method and collects literature using internet sources from the Google Scholar database. The stages consist of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that fluorescent pseudomonads are potential and effective in controlling various plant diseases.
Fluorescent Pseudomonad Association in Plant Rhizosphere Intan Febriani; Linda Advinda; Dezi Handayani; Siska Alicia Farma; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Plant growth promoting rhizobacteria or known as Plant Growth Promoting Rhizobacteria (PGPR) are bacteria found on the surface of plant roots and are beneficial microorganisms. The rhizosphere is an ideal area for the growth and development of soil microorganisms. This situation is supported by its function, namely as a provider of nutrients and also as a place for the growth and development of microorganisms. One of the bacteria found in the rhizosphere of plants is the fluorescent pseudomonad. Fluorescent pseudomonads are biological agents that can be isolated from the surface area of plant roots. This study aims to collect and analyze articles related to the association of fluorescent pseudomonads from plant rhizosphere. This type of research is research that uses the literature review method and literature collection using internet sources from the Google Scholar database. This stage consists of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that the use of fluorescent pseudomonads as a biological control agent has been widely carried out because of their ability to produce antimicrobial compounds.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul AERMA HASTUTY Afionita, Santi Ahmad Wibisana, Ahmad Albar, Rahmat Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Andri Damayanti, Ndaru Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Az-Zahra, Fauziah Azwir Anhar Berlinda Paradisa, Yashanti Cania Dewi, Rahmawitra Chatri, Moralita Chatri Cindy Pramila Darussyamsu, Rahmawati Des M Dezi Handayani Dezi Handayani Dina Sukma dina vaniana Dinda Sahara Djoelvinanda, Habibullah Edwin Edwin Efliani Efliani Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fahra Fahra Fahra, Fahra Fatma Rahmadhani Febri Doni Febrina Anggiastanti Feby Yeriska Fevria, Resti Fira Safitri Fransisco, Sandi Fronica, Imelda Ghiffari, Muhammad Gustina Indriati Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Kiki Amelia, Kiki Larasati Arum Utami Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Mantoviana, Tiffany Marissa Angelina Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini, Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhlis Mukhlis Mukhtar Mukhtar Mulia Mulia Mulia Mutia Anika mutia anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Nisa Afifah Novitasari, Yuliana Diyah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nurfadillatun Nisa Wijaya Nurfatihah Z, Zahara Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Pratama, Sandi Fransisco Prima, Rika Putri Rachma Auliya Putri, Aulia Devani Putri, Cici Adelia Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal safitri, fira Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Sari, Rinti Mutiara Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh SOLICHATUN SOLICHATUN Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Tomi Apra Santosa Tomi Apra Santosa Santosa Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda Wahyuni Wahyuni wahyuni wahyuni Wahyuni, Resma Widya Ruchi Wilna Sari Wirda Taufik Y Yusrizal Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri