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INTERAKSI SINERGIS DALAM KONSORSIUM TERMOFILIK: MENYEIMBANGKAN RASIO KONSENTRASI MIKROBA DALAM PRODUKSI BIOELEKTRIK Alfatimah Azzahra, Balkis; Irdawati; Handayani, Dezi; Hilda Putri, Dwi
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6048

Abstract

The global urgency for sustainable energy solutions amid climate change and fossil fuel depletion has driven innovation in Microbial Fuel Cells (MFCs). This study investigates the synergistic interactions within a thermophilic microbial consortium (MS9 & MS12) to optimize energy efficiency in MFCs. By varying the inoculum ratios (1:1, 1:2, and 1:3), the research evaluates how microbial population balance impacts bioelectricity production. The MFCs were operated for 24 hours, with voltage measurements recorded every 2 hours. Results revealed that a 1:3 (MS9:MS12) ratio achieved the highest voltage output, peaking at 20 hours before declining, indicating metabolic synergy and efficient substrate utilization. Descriptive analysis highlighted the critical role of cross-feeding mechanisms and functional complementarity between species in enhancing electron transfer. The findings align with modular co-cultivation principles, emphasizing the importance of engineered microbial ratios for stable system performance. This study provides a protocol for designing balanced thermophilic consortia, advancing sustainable bioenergy strategies through optimized microbial interactions.
Potential of Lactic Acid Bacteria Isolated from Fermented Tempe Food in Producing Fibrinolytic Enzymes Amanda, Nifsa Riski; Hastuty, Aerma; Putri, Dwi Hilda; Sulistiani, Sulistiani
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9554

Abstract

Cardiovascular disease is the leading cause of death worldwide, mainly due to blood vessel blockage caused by thrombus. Fibrinolytic enzymes are used to dissolve thrombus but are often limited by high production costs, low specificity, allergic reactions, and bleeding risks. As a safer alternative, bacteria from fermented foods offer several advantages, including low toxicity, cost-effective production, and high fibrin specificity, which reduces the risk of bleeding. This study investigated the potential and characteristics of fibrinolytic enzyme activity produced by lactic acid bacteria (LAB) isolated from tempeh. The fibrin plate method was used to identify LAB producing the enzyme, while enzyme activity was measured using the method of Varol et al. (2023), and protein concentration was analyzed using a modified Bradford test. Thirteen LAB isolates showed fibrinolytic activity, with isolate PG01-2B selected as the most promising. The highest enzyme production occurred on the second day, reaching 83.41 U/mL. Characterization showed activity with acetic acid buffer pH 6 (102.18 U/mL) and at 75 minutes incubation time (99 U/mL). These results indicate that LAB from tempeh has strong potential as a source of fibrinolytic enzymes and can function as an alternative therapeutic agent for thrombus-related cardiovascular diseases.
Pemanfaatan Sisa Buah dan Sayur sebagai Produk ECOBY Ecoenzyme di Kampus Universitas Negeri Padang Farma, Siska Alicia; Handayani, Dezi; Putri, Irma Leilani Eka; Putri, Dwi Hilda
Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Vol 21, No 2 (2021): Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/sb.01180

Abstract

Setiap hari aktivitas masyarakat termasuk masyarakat kampus (dosen, pegawai, dan mahasiswa) selalu menghasilkan limbah organik dan anorganik. Limbah dapat berasal dari sisa makanan sivitas akademika UNP maupun sisa bahan praktikum-praktikum di laboratorium biologi dan kimia FMIPA UNP. Ketika lingkungan kotor maka tidak sedikit tim cleaning service di UNP yang kewalahan. Tujuan kegiatan ini adalah bagaimana pengolahan sisa sayur dan buah di lingkungan kampus menjadi produk bermanfaat. Solusi yang ditawarkan melalui pengabdian ini adalah mengolah sampah makanan khususnya buah dan sayuran menjadi larutan ecoenzyme yang dapat digunakan untuk berbagai keperluan bagi kesehatan dan lingkungan. Oleh karena itu sangatlah tepat jika hadirnya suatu pusat produksi ecoenzyme di jurusan biologi FMIPA UNP. ECOBY adalah merek dagang ecoenzyme yang dibuat oleh unit usaha PPUPIK Jurusan Biologi FMIPA UNP berkerjasama dengan Tim Mahasiswa HMJ Biologi. Proses pembuatan ecoenzyme berasal dari sampah sayur dan kulit buah, gula merah dan air dengan perbandingan 3:1:10. Proses ini membutuhkan waktu 90-100 hari minimal. Sisa buah yang digunakan adalah kulit apel, jeruk, nanas, pir, semangka, lemon, melon, mangga, alpukat yang diperoleh dari lingkungan sekitar UNP. Ecoenzyme mempercepat reaksi biokimia di alam untuk menghasilkan enzim yang berguna untuk berbagai kebutuhan sehari-hari. 
Activity of Ethanol Extract and Fraction Products Leaves Manilkara kauki as Inhibitors Tyrosinase Enzyme Zakri, Dwika Febriana; Putri, Dwi Hilda; Irdawati, Irdawati; Angelina, Marissa
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8295

Abstract

Currently, cosmetics made from chemical (synthetic) ingredients are in demand by the public because they can whiten the skin by inhibiting the formation of melanin, but the lack of public knowledge about the impact of excessive use has encouraged the need for natural ingredients as tyrosinase inhibitors that are safer than synthetic ingredients. This study aims to test the potential of secondary metabolite compounds in M. kauki leaves as inhibitors of tyrosinase enzyme activity by determining the IC50 value. This assay utilizes L-tyrosine and arbutin as positive control substrates, with UV-Vis spectrophotometric absorption measurements taken at a wavelength of 470 nm. The findings revealed that the buthanol fraction exhibited the highest tyrosinase enzyme inhibition, with an IC50 of 189,72 μg/mL. This was followed by the ethanol extract with an IC50 of 191,97 μg/mL, the hexane fraction at 381.50 μg/mL, and the ethyl acetate extract with an IC50 of 448.986 μg/mL. All samples displayed strong inhibitory activity, outperforming arbutin as a positive control, which had an IC50 value of 831.51 μg/mL.
Dextran Producing Bacteria: A Literatur Review Salsabilla, Vishtari; Putri, Dwi Hilda; Advinda, Linda; Achyar, Afifatul; Wibisana, Ahmad
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8370

Abstract

This study aims to analyze dextran-producing bacteria, especially from the general Leuconostoc, Weissella, and Lactobacillus, which produce the enzyme dextransucrase for dextran synthesis. This research uses the Systematic Literature Review (SLR) method based on the PRISMA protocol, with data sources from various trusted scientific databases for the 2020–2024 period. The results of the study show that variations in dextran structure, such as molecular weight and degree of branching, are highly dependent on the bacterial strain and fermentation conditions. Optimization of production parameters, including pH, temperature and substrate concentration, can increase dextran production efficiency The conclusions of this study emphasize the need for a multidisciplinary approach to optimize the potential of dextran in industrial and biotechnology applications. Further research is recommended to explore new strains, optimize production processes, and diversify dextran applications.
Literatur Review: D-Allulose 3-Epimerase from Microbial Sources and its Potential Uses Az-Zahra, Fauziah; Putri, Dwi Hilda; Irdawati, Irdawati; Handayani, Dezi
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8402

Abstract

D-allulose, or psicose, is a rare, low-calorie sugar with various health benefits, such as managing blood sugar levels and lowering the risk of obesity, making it an ideal alternative to sucrose. In the food and pharmaceutical sectors, this study aims to review the characteristics and potential of D-allulose 3-epimerase (DAEase) enzymes from different microbial sources, along with the challenges and prospects associated with industrial-scale applications. The methodology involved a literature review to analyse the properties of DAEase enzymes, including specific activity, thermal stability, and optimal temperature and pH conditions from different microbial producers. The results showed that the enzyme from Agrobacterium sp. exhibited the highest specific activity, while the enzyme from Labedella endophytica offered the best thermal stability. In conclusion, DAEase enzymes show significant potential in the production of D-allulose, especially in supporting the global trend towards healthier and more environmentally friendly low-calorie food products. Further research is recommended to focus on improving production efficiency and technology development for large-scale commercial applications.
AKTIVITAS SPESIFIK XILANASE BAKTERI TERMOFILIK AMOBIL SSA2 DENGAN VARIASI SUMBER KARBON Irdawati, Irdawati; Nurfatihah Z, Zahara; Putri, Dwi Hilda; Advinda, Linda; Yusrizal, Yusrizal
Jurnal Agrotek Tropika Vol. 13 No. 1 (2025): JURNAL AGROTEK TROPIKA VOL 13, FEBRUARI 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i1.7130

Abstract

Enzim xilanase memiliki potensi besar dalam berbagai industri. Salah satu kegunaannya adalah dalam industri farmasi dan makanan karena kemampuannya mengubah xilan menjadi xilosa, sebuah gula rendah kalori. Selain itu, enzim xilanase juga digunakan dalam industri kertas sebagai agen pemutihan pulp (biobleaching). Xilanase dapat dihasilkan oleh bakteri termofilik dengan menggunakan xilan dari ekstrak jerami padi sebagai substrat. Produksi enzim dipengaruhi oleh nutrisi yang tersedia, seperti sumber karbon. Penelitian ini dilakukan untuk menentukan sumber karbon terbaik untuk meningkatkan aktivitas spesifik xilanase oleh bakteri termofilik amobil SSA2. Untuk menghitung aktivitas khusus xilanase, dibagi aktivitas xilanase dengan konsentrasi protein. Penelitian dilakukan di laboratorium departemen biologi FMIPA UNP dari November 2021 hingga Januari 2022 dengan menggunakan metode eksperimen yang mengikuti rancangan acak lengkap (RAL) dan melibatkan 5 perlakuan yang diulang sebanyak 3 kali. Temuan dari penelitian tersebut menunjukkan bahwa penambahan xilosa sebagai sumber karbon menghasilkan aktivitas khusus enzim xilanase tertinggi sebesar 1,536 Unit/mg. Kata kunci:  Xylanase, Sumber Karbon, Xylosa, Bakteri Termofilik. 
Primer Design and Optimization of Annealing Temperature for Gene Amplification GSTL2 on Rice Violita, Violita; Achyar, Afifatul; Zulyusri, Zulyusri; Atifah, Yusni; Putri, Dwi Hilda; Nabilah, Rezi
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.32859

Abstract

AbstractGlutathione S-Transferase is a superfamily enzyme that has many roles for living things, especially in the detoxification of Reactive Oxygen Species (ROS), one of which is rice plants. One gene of this family that has a role for plants is GSTL2 This gene is known to have a contribution to Arabidopsis and Oryza sativa L. against abiotic stress. To find out how this gene expression is requires a primer to specifically amplify this gene, and an optimal annealing temperature to support the success of the primer. This study aims to design primers and determine the optimal annealing temperature for gene amplification GSTL2. Primers were designed using the Primer3, which were then analyzed with Genious Prime based on good primer criteria and optimizing the annealing temperature which was carried out using gradient PCR. The results of this study obtained a primer pair is forward 5’-TTCGAAGGGCCAGCATTACT-3’ primer reverse 5’-CAATGTCCACCAAGCTGAA-3’. The primer pair has a length of 20 nt, with melting temperature (Tm) 59–59,4 °C, and GC content 50%. The primer forward there is a secondary structure in the form of a self-dimer with (Tm) 6.2 °C. The primer pair can amplify gene sequences GSTL2 by producing a PCR product 224 bp. The annealing temperature of 60 °C resulting in a single thick, bright DNA strand.AbstrakGlutathione S-Transferase merupakan enzim superfamily yang memiliki banyak peranan bagi makhluk hidup terutama dalam detoksifikasi Reactive Oxygen Species (ROS), salah satunya tumbuhan. Salah satu gen dari famili ini yang memiliki peranan bagi tanaman adalah GSTL2. Gen ini diketahui memiliki kontribusi bagi tanaman Arabidopsis dan Oryza sativa L. terhadap stres abiotik. Untuk mengetahui bagaimana ekspresi gen GSTL2 ini dibutuhkan primer untuk mengamplifikasi gen ini secara spesifik, di samping itu suhu annealing yang optimal untuk menunjang keberhasilan primer. Penelitian ini bertujuan untuk mendesain primer dan menentukan suhu annealing yang optimal untuk mengamplifikasi gen GSTL2. Primer didesain menggunakan tools Pick Primer dan Geneious Prime, yang kemudian dianalisis secara in silico berdasarkan kriteria primer yang baik. Serta optimasi suhu annealing yang dilakukan menggunakan gradient PCR. Hasil penelitian ini diperoleh sepasang primer dengan panjang masing-masing primer 20 nt, primer forward 5’-TTCGAAGGGCCAGCATTACT-3’ primer reverse 5’-CAATGTCCACCAAGCTGAA-3’. Pasangan primer dapat mengamplifikasi sekuen gen GSTL2 dengan menghasilkan produk PCR sebesar 224 bp pada suhu annealing 60 °C.
Peningkatan Kemampuan Guru MGMP MA Biologi Sumatera Barat dalam Mengimplementasikan Materi Bioteknologi Berbasis Bioentrepreneurship Handayani, Dezi; putri, dwi hilda; Atifah, Yusni; Eka Putri, Irma Leilani; Chatri, Moralita; Fransisco, Sandi
Pelita Eksakta Vol 7 No 1 (2024): Pelita Eksakta, Vol. 7, No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol7-iss1/226

Abstract

Terkait dengan taget Indonesia untuk mencapai kemandirian dibidang ekonomi pada tahun 2015-2085, maka penting usaha mendorong munculnya entreprenurship baru dari kalangan muda. Entrepreneurship dikalanganan anak muda usia sekola dapat dibelajarkan dengan menerapkan entrepreneurship pada mata pelajaran bioteknologi. Pengintegrasian muatan nilai-nilai kewirausahaan kedalam bidang ilmu hayati seperti bioteknologi dikenal dengan istilah bioentrepreneurship. Kendala yang dihadapi oleh guru biologi di MGMP MA Sumbar adalah masih rendah tentang aplikasi teknik produksi produk bioteknologi. Keterbatasan juga dimiliki guru mengenai potensi produk bioteknologi yang mengandalkan kekayaan alam Sumatera Barat. Permasalahan inilah yang menjadi dasar pelatihan pembuatan produk bioteknologi berbasis bioentreprenur. Kegiatan di hadiri oleh 40 guru yang tergabung dalam MGMP MA Biologi Sumatera Barat. Pelatihan menghadirkan 3 orang narasumber yang berkopeten. Kegiatan ini tentu diharapkan adanya peningkatakan kemampuan guru-guru MGMP dalam praktek metode bioteknologi untuk menumbuhkan jiwa bioentrepreneurship generasi muda.
Training by the PUPIK Team, FMIPA UNP Biology Laboratory, on Making Nata De Coco and Production Quality Testing During the Storage Process putri, dwi hilda; Advinda, Linda; Irdawati, Irdawati; Fevria, Resti; Chatri, Moralita; rahmi, elva; Ghiffari, Muhammad; Viona, Alda
Pelita Eksakta Vol 6 No 1 (2023): Pelita Eksakta Vol. 6 No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol6-iss1/252

Abstract

Nata de coco is one way to use coconut water waste that can no longer be used so that it can reduce environmental pollution. The quality of nata as a food and beverage product must be monitored. This article aims to analyze the shelf life of Nata de coco based on its shelf life. The work methods used include: 1) testing the durability of nata de coco at room temperature, and 2) testing the durability of nata de coco at cold temperatures or in the refrigerator. Organoleptic tests carried out included aroma, color, taste and texture. The results achieved show that nata de coco stored in the refrigerator can last four weeks, while those stored at room temperature can only last for one week
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul ADE ANDRIANI AERMA HASTUTY Afifatul Achyar Afionita, Santi Afroza, Faiza Ahmad Wibisana, Ahmad Albar, Rahmat Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Az-Zahra, Fauziah Azwir Anhar Berlinda Paradisa, Yashanti Cania Dewi, Rahmawitra Chatri, Moralita Chatri Cindy Pramila Darussyamsu, Rahmawati Des M Dezi Handayani Dezi Handayani Dina Sukma dina vaniana Dinda Sahara Djoelvinanda, Habibullah Echa Azkia Afandi Edwin Edwin Efliani Efliani Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fahra Fahra Fatma Rahmadhani Febri Doni Febrina Anggiastanti Feby Yeriska Fevria, Resti Fhuji Winardi Fhuji Winardi Fira Safitri Fira Safitri Fransisco, Sandi Fronica, Imelda Ghiffari, Muhammad Gustina Indriati Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal IWAN SASKIAWAN Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Kiki Amelia, Kiki Larasati Arum Utami Linda Advinda Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Marissa Angelina Maulia Indah Ayuningtyas Mellani Rachma Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhlis Mukhlis Mukhtar Mukhtar Mulia Mulia Mulia mutia anika Mutia Anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Ndaru Andri Damayanti Nisa Afifah Novitasari, Yuliana Diyah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nurfadillatun Nisa Wijaya Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Pratama, Sandi Fransisco Prima, Rika Putri Rachma Auliya Putri, Aulia Devani Putri, Cici Adelia Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resma Wahyuni Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Siska Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh SOLICHATUN SOLICHATUN Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Tiffany Mantoviana Tomi Apra Santosa Tomi Apra Santosa Santosa Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda Wahyuni Wahyuni wahyuni wahyuni Widya Ruchi Wilna Sari Wirda Taufik Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Zahara Nurfatihah Z Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri