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All Journal Jurnal Biologi Udayana Bioscience Bioeducation Journal Jurnal Ilmu Dasar AL KAUNIYAH Jurnal Biotek Medisiana Indonesia Althea Medical Journal Biota Biomedika BIO-SITE |BIOLOGI Sains Terapan Jurnal Penelitian dan Pembelajaran IPA Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Jurnal Agrotek Tropika Bio-Lectura Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Ilmu Kefarmasian Indonesia AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Majalah Kesehatan Pharmamedika Pelita Eksakta Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi IJECA (International Journal of Education and Curriculum Application) Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Biosense Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Tropical Genetics Jurnal Serambi Biologi Berita Biologi
Dwi Hilda Putri
Universitas Negeri Padang
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Genetic Variation Analysis of L1 gene HPV-16 using RFLP in Silico Afionita, Santi; Putri, Dwi Hilda; Achyar, Afifatul; Yuniarti, Elsa
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8415

Abstract

Human papillomavirus (HPV) is one of the leading causes of cervical cancer, with the L1 gene playing an important role in vaccine development. This study aims to analyze the genetic variation of the L1 gene of HPV types 16, 18, 52, and 58 using the RFLP method in silico by utilizing restriction enzymes. The results showed that. The pattern of genetic conservation is more dominant in HPV 16 and 52 than other types, indicating the potential important role of the L1 gene in vaccine development. This study confirms the importance of analyzing genetic variation to understand HPV genetic diversity. These results may contribute to the development of more specific vaccines and molecular markers for HPV detection.
Correlation between Environmental Factors and Morphological Characteristics of Koenih Rimbo (Curcuma sumatrana Miq.) as an Endangered Plant in West Sumatra Jannah, Miftahul; Putri, Irma Leilani Eka; Kardiman, Reki; Anhar, Azwir; Putri, Dwi Hilda; Pratama, Chelsylia Dara; Valofi, Nagra Aulia; Putri, Cici Adelia; Aisyah, Siti; Djoelvinanda, Habibullah
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8604

Abstract

Curcuma sumatrana or Koenih Rimbo, is an endemic plant of West Sumatra that is threatened with extinction due to deforestation, climate change, and human activities. This study aims to understand the ecology of C. sumatrana to support effective conservation efforts. The study was conducted in seven potential locations in West Sumatra using exploration methods and analysis of environmental factors, such as altitude, temperature, humidity, soil pH, and canopy cover. The results showed that this species was only found in three locations, namely Lubuk Kilangan, Koto Malintang, and Koto Pulai, with the highest population in Koto Malintang. Environmental factors such as altitude, canopy cover, and air humidity were shown to support optimal growth of this species. Canonical Correspondence Analysis (CCA) identified a close relationship between environmental factors and morphological parameters, including plant height, number of branches, and leaf size. This study emphasizes the importance of natural habitat conservation and further research on the ecology of this species to prevent its extinction.
INTERAKSI SINERGIS DALAM KONSORSIUM TERMOFILIK: MENYEIMBANGKAN RASIO KONSENTRASI MIKROBA DALAM PRODUKSI BIOELEKTRIK Alfatimah Azzahra, Balkis; Irdawati; Handayani, Dezi; Hilda Putri, Dwi
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6048

Abstract

The global urgency for sustainable energy solutions amid climate change and fossil fuel depletion has driven innovation in Microbial Fuel Cells (MFCs). This study investigates the synergistic interactions within a thermophilic microbial consortium (MS9 & MS12) to optimize energy efficiency in MFCs. By varying the inoculum ratios (1:1, 1:2, and 1:3), the research evaluates how microbial population balance impacts bioelectricity production. The MFCs were operated for 24 hours, with voltage measurements recorded every 2 hours. Results revealed that a 1:3 (MS9:MS12) ratio achieved the highest voltage output, peaking at 20 hours before declining, indicating metabolic synergy and efficient substrate utilization. Descriptive analysis highlighted the critical role of cross-feeding mechanisms and functional complementarity between species in enhancing electron transfer. The findings align with modular co-cultivation principles, emphasizing the importance of engineered microbial ratios for stable system performance. This study provides a protocol for designing balanced thermophilic consortia, advancing sustainable bioenergy strategies through optimized microbial interactions.
Potential of Lactic Acid Bacteria Isolated from Fermented Tempe Food in Producing Fibrinolytic Enzymes Amanda, Nifsa Riski; Hastuty, Aerma; Putri, Dwi Hilda; Sulistiani, Sulistiani
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9554

Abstract

Cardiovascular disease is the leading cause of death worldwide, mainly due to blood vessel blockage caused by thrombus. Fibrinolytic enzymes are used to dissolve thrombus but are often limited by high production costs, low specificity, allergic reactions, and bleeding risks. As a safer alternative, bacteria from fermented foods offer several advantages, including low toxicity, cost-effective production, and high fibrin specificity, which reduces the risk of bleeding. This study investigated the potential and characteristics of fibrinolytic enzyme activity produced by lactic acid bacteria (LAB) isolated from tempeh. The fibrin plate method was used to identify LAB producing the enzyme, while enzyme activity was measured using the method of Varol et al. (2023), and protein concentration was analyzed using a modified Bradford test. Thirteen LAB isolates showed fibrinolytic activity, with isolate PG01-2B selected as the most promising. The highest enzyme production occurred on the second day, reaching 83.41 U/mL. Characterization showed activity with acetic acid buffer pH 6 (102.18 U/mL) and at 75 minutes incubation time (99 U/mL). These results indicate that LAB from tempeh has strong potential as a source of fibrinolytic enzymes and can function as an alternative therapeutic agent for thrombus-related cardiovascular diseases.
Optimization of Authentication Methods for Processed Chicken Meat Products Based on ND5 Gene qPCR Achyar, Afifatul; Safitri, Fira; Atifah, Yusni; Farma, Siska Alicia; Putri, Dwi Hilda; Ahda, Yuni
Halal Research Vol 5 No 2 (2025): July
Publisher : Halal Center ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j22759970.v5i2.1823

Abstract

Consumers have the right to correct information about the products they consume. One example of a food product that is vulnerable to counterfeiting is chicken nuggets. To overcome this problem, qPCR can be an effective solution. Previous research has designed primers targeting the chicken ND5 gene. However, the PCR conditions for this primer pair have never been optimized. This research aims to optimize qPCR conditions and carry out chicken nugget authentication trials. Genomic DNA was isolated from five weight variations of chicken meat samples as a qPCR standard curve and three chicken nugget samples. Optimizing the annealing temperature with gradient PCR gave the best results at a temperature of 57.6OC. The results of the authentication test showed that the chicken meat content in TD nuggets was 54.7%, C nuggets 43.8%, and D nuggets 37.3%. This value is still in accordance with the Indonesian National Standard (SNI).
Pemanfaatan Sisa Buah dan Sayur sebagai Produk ECOBY Ecoenzyme di Kampus Universitas Negeri Padang Farma, Siska Alicia; Handayani, Dezi; Putri, Irma Leilani Eka; Putri, Dwi Hilda
Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Vol 21, No 2 (2021): Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/sb.01180

Abstract

Setiap hari aktivitas masyarakat termasuk masyarakat kampus (dosen, pegawai, dan mahasiswa) selalu menghasilkan limbah organik dan anorganik. Limbah dapat berasal dari sisa makanan sivitas akademika UNP maupun sisa bahan praktikum-praktikum di laboratorium biologi dan kimia FMIPA UNP. Ketika lingkungan kotor maka tidak sedikit tim cleaning service di UNP yang kewalahan. Tujuan kegiatan ini adalah bagaimana pengolahan sisa sayur dan buah di lingkungan kampus menjadi produk bermanfaat. Solusi yang ditawarkan melalui pengabdian ini adalah mengolah sampah makanan khususnya buah dan sayuran menjadi larutan ecoenzyme yang dapat digunakan untuk berbagai keperluan bagi kesehatan dan lingkungan. Oleh karena itu sangatlah tepat jika hadirnya suatu pusat produksi ecoenzyme di jurusan biologi FMIPA UNP. ECOBY adalah merek dagang ecoenzyme yang dibuat oleh unit usaha PPUPIK Jurusan Biologi FMIPA UNP berkerjasama dengan Tim Mahasiswa HMJ Biologi. Proses pembuatan ecoenzyme berasal dari sampah sayur dan kulit buah, gula merah dan air dengan perbandingan 3:1:10. Proses ini membutuhkan waktu 90-100 hari minimal. Sisa buah yang digunakan adalah kulit apel, jeruk, nanas, pir, semangka, lemon, melon, mangga, alpukat yang diperoleh dari lingkungan sekitar UNP. Ecoenzyme mempercepat reaksi biokimia di alam untuk menghasilkan enzim yang berguna untuk berbagai kebutuhan sehari-hari. 
Activity of Ethanol Extract and Fraction Products Leaves Manilkara kauki as Inhibitors Tyrosinase Enzyme Zakri, Dwika Febriana; Putri, Dwi Hilda; Irdawati, Irdawati; Angelina, Marissa
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8295

Abstract

Currently, cosmetics made from chemical (synthetic) ingredients are in demand by the public because they can whiten the skin by inhibiting the formation of melanin, but the lack of public knowledge about the impact of excessive use has encouraged the need for natural ingredients as tyrosinase inhibitors that are safer than synthetic ingredients. This study aims to test the potential of secondary metabolite compounds in M. kauki leaves as inhibitors of tyrosinase enzyme activity by determining the IC50 value. This assay utilizes L-tyrosine and arbutin as positive control substrates, with UV-Vis spectrophotometric absorption measurements taken at a wavelength of 470 nm. The findings revealed that the buthanol fraction exhibited the highest tyrosinase enzyme inhibition, with an IC50 of 189,72 μg/mL. This was followed by the ethanol extract with an IC50 of 191,97 μg/mL, the hexane fraction at 381.50 μg/mL, and the ethyl acetate extract with an IC50 of 448.986 μg/mL. All samples displayed strong inhibitory activity, outperforming arbutin as a positive control, which had an IC50 value of 831.51 μg/mL.
Dextran Producing Bacteria: A Literatur Review Salsabilla, Vishtari; Putri, Dwi Hilda; Advinda, Linda; Achyar, Afifatul; Wibisana, Ahmad
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8370

Abstract

This study aims to analyze dextran-producing bacteria, especially from the general Leuconostoc, Weissella, and Lactobacillus, which produce the enzyme dextransucrase for dextran synthesis. This research uses the Systematic Literature Review (SLR) method based on the PRISMA protocol, with data sources from various trusted scientific databases for the 2020–2024 period. The results of the study show that variations in dextran structure, such as molecular weight and degree of branching, are highly dependent on the bacterial strain and fermentation conditions. Optimization of production parameters, including pH, temperature and substrate concentration, can increase dextran production efficiency The conclusions of this study emphasize the need for a multidisciplinary approach to optimize the potential of dextran in industrial and biotechnology applications. Further research is recommended to explore new strains, optimize production processes, and diversify dextran applications.
Literatur Review: D-Allulose 3-Epimerase from Microbial Sources and its Potential Uses Az-Zahra, Fauziah; Putri, Dwi Hilda; Irdawati, Irdawati; Handayani, Dezi
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8402

Abstract

D-allulose, or psicose, is a rare, low-calorie sugar with various health benefits, such as managing blood sugar levels and lowering the risk of obesity, making it an ideal alternative to sucrose. In the food and pharmaceutical sectors, this study aims to review the characteristics and potential of D-allulose 3-epimerase (DAEase) enzymes from different microbial sources, along with the challenges and prospects associated with industrial-scale applications. The methodology involved a literature review to analyse the properties of DAEase enzymes, including specific activity, thermal stability, and optimal temperature and pH conditions from different microbial producers. The results showed that the enzyme from Agrobacterium sp. exhibited the highest specific activity, while the enzyme from Labedella endophytica offered the best thermal stability. In conclusion, DAEase enzymes show significant potential in the production of D-allulose, especially in supporting the global trend towards healthier and more environmentally friendly low-calorie food products. Further research is recommended to focus on improving production efficiency and technology development for large-scale commercial applications.
AKTIVITAS SPESIFIK XILANASE BAKTERI TERMOFILIK AMOBIL SSA2 DENGAN VARIASI SUMBER KARBON Irdawati, Irdawati; Nurfatihah Z, Zahara; Putri, Dwi Hilda; Advinda, Linda; Yusrizal, Yusrizal
Jurnal Agrotek Tropika Vol. 13 No. 1 (2025): JURNAL AGROTEK TROPIKA VOL 13, FEBRUARI 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i1.7130

Abstract

Enzim xilanase memiliki potensi besar dalam berbagai industri. Salah satu kegunaannya adalah dalam industri farmasi dan makanan karena kemampuannya mengubah xilan menjadi xilosa, sebuah gula rendah kalori. Selain itu, enzim xilanase juga digunakan dalam industri kertas sebagai agen pemutihan pulp (biobleaching). Xilanase dapat dihasilkan oleh bakteri termofilik dengan menggunakan xilan dari ekstrak jerami padi sebagai substrat. Produksi enzim dipengaruhi oleh nutrisi yang tersedia, seperti sumber karbon. Penelitian ini dilakukan untuk menentukan sumber karbon terbaik untuk meningkatkan aktivitas spesifik xilanase oleh bakteri termofilik amobil SSA2. Untuk menghitung aktivitas khusus xilanase, dibagi aktivitas xilanase dengan konsentrasi protein. Penelitian dilakukan di laboratorium departemen biologi FMIPA UNP dari November 2021 hingga Januari 2022 dengan menggunakan metode eksperimen yang mengikuti rancangan acak lengkap (RAL) dan melibatkan 5 perlakuan yang diulang sebanyak 3 kali. Temuan dari penelitian tersebut menunjukkan bahwa penambahan xilosa sebagai sumber karbon menghasilkan aktivitas khusus enzim xilanase tertinggi sebesar 1,536 Unit/mg. Kata kunci:  Xylanase, Sumber Karbon, Xylosa, Bakteri Termofilik. 
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul AERMA HASTUTY Afionita, Santi Ahmad Wibisana, Ahmad Albar, Rahmat Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Andri Damayanti, Ndaru Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Az-Zahra, Fauziah Azwir Anhar Berlinda Paradisa, Yashanti Cania Dewi, Rahmawitra Chatri, Moralita Chatri Cindy Pramila Darussyamsu, Rahmawati Des M Dezi Handayani Dezi Handayani Dina Sukma dina vaniana Dinda Sahara Djoelvinanda, Habibullah Edwin Edwin Efliani Efliani Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fahra Fahra Fahra, Fahra Fatma Rahmadhani Febri Doni Febrina Anggiastanti Feby Yeriska Fevria, Resti Fira Safitri Fransisco, Sandi Fronica, Imelda Ghiffari, Muhammad Gustina Indriati Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Kiki Amelia, Kiki Larasati Arum Utami Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Mantoviana, Tiffany Marissa Angelina Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini, Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhlis Mukhlis Mukhtar Mukhtar Mulia Mulia Mulia mutia anika Mutia Anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Nisa Afifah Novitasari, Yuliana Diyah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nurfadillatun Nisa Wijaya Nurfatihah Z, Zahara Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Pratama, Sandi Fransisco Prima, Rika Putri Rachma Auliya Putri, Aulia Devani Putri, Cici Adelia Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal safitri, fira Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Sari, Rinti Mutiara Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh SOLICHATUN SOLICHATUN Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Tomi Apra Santosa Tomi Apra Santosa Santosa Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda Wahyuni Wahyuni wahyuni wahyuni Wahyuni, Resma Widya Ruchi Wilna Sari Wirda Taufik Y Yusrizal Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri