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All Journal Jurnal Biologi Udayana Bioscience Bioeducation Journal Jurnal Ilmu Dasar AL KAUNIYAH Jurnal Biotek Medisiana Indonesia Althea Medical Journal Biota Biomedika BIO-SITE |BIOLOGI Sains Terapan Jurnal Penelitian dan Pembelajaran IPA Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Jurnal Agrotek Tropika Bio-Lectura Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Ilmu Kefarmasian Indonesia AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Majalah Kesehatan Pharmamedika Pelita Eksakta Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi IJECA (International Journal of Education and Curriculum Application) Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Biosense Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Tropical Genetics Jurnal Serambi Biologi

Dwi Hilda Putri

Universitas Negeri Padang

Author-ID : 2726338

Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Respon Pertumbuhan Benih Padi Varietas Anak Daro Asal Solok Terhadap Isolat Trichoderma Indeginous Azwir Anhar; Dwi HIlda Putri; Febri Doni; Linda Advinda
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Trichoderma spp adalah jamur non patogen yang berasosiasi dengan rhizosfer dan daun tanaman. Jamur ini juga mampu mengendalikan penyakit dan menghasilkan zat pengatur tumbuh, sehingga potensial digunakan dalam pertaniankhususnya dalam budidaya pertanian organik. Penelitian kemampuan Trichoderma spp dalam meningkatkan pertumbuhan benih padi masih terbatas. Penelitian ini, melaporkan kemampuan Trichoderma setempat untuk meningkatkan poertumbuhan kecambah benih padi. Hasil penelitian menunjukkan bahwa isolat Trichoderma mampu meningkatkan daya kecambah dan vigor benih padi. Hasil penelitian membuktikan bahwa isolat Trichoderma indegenous memiliki potensi untuk digunakan sebagai pengatur pertumbuhan benih padi. Hasil penelitian meningkatkan pemahaman lebih lanjut tentang peranan jamur dalam meningkatkan pertumbuhan padi

Isolation and Identification of Endophytic Bacteria from the Andalas Plant Stem (Morus macroura Miq.) Nisa Afifah; Dwi Hilda Putri; Irdawati Irdawati
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The number of death couse of infection continues to increase. Treatment of infections is increasingly difficult due to increased resistance of germs to existing antimicrobial compounds. A new source of antimicrobial substances is needed. One source of antimicrobial substances is endophytic bacteria that live in the stems of Andalas plants. The aim of this study was to compare the diversity of endophytic bacteria present in old and young Andalas plant stems. The method used to isolate endophytic bacteria from the stem of the Andalas plant is the streak plate method. Bacterial identification is done macroscopically and microscopically. The results of macroscopic observation showed that there are differences in the number of isolates found in old and young Andalas plant stems. 11 isolates of endophytic bacteria from Andalas plant stems, each of which consists of 9 endophytic bacteria from old Andalas stems and two isolates from young Andalas stems. A microscopic identification was found that of the 11 isolates isolated, eight isolates were gram-positive and three isolates were gram-negative bacteria

The Effect Of Time Differences On Polyacrilamide Gel Electrophoresis On Cassava DNA Nur Ayu Ramadanti; Dwi Hilda Putri
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

DNA bands formed from the results of electrophoresis with Polyacrilamide gel are considered as 1 character representing 1 allele. PCR products produce multiple bands (multy bands), which indicates that there are multi alleles in the sample. Electrophoresis is a chemical analysis method based on the movement of charged protein molecules in the electric field. Separation is carried out based on differences in the size of the molecular weight and the electric charge contained by the macro-molecule. In addition, the effect of gel concentratio n, buffer and electrophoresis time also has a role in the results of electrophoresis. This study was conducted to compare the best separation time for acrilamide gel electrophoresis with the results of cassava DNA amplification. The materials used in this study are two cassava varieties, namely: Adira IV 1, Adira IV 2, Adira IV 3, Carvita 25 1, Carvita 25 2, and Carvita 25 3. Using electrophoresis by poly-acrilamide gel with two different time effects: 1 hour 30 minutes and 3 hours. The results of electrophoresis with 3 hours gave better results of DNA visualization compared to 1 hour 30 minutes.

Comparison of Three Different DNA Isolation Methods To Degradate The Trichoderma Fungi Cell Wall Widya Ruchi; Dwi Hilda Putri; Azwir Anhar
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Dinding sel jamur Trichoderma tersusun atas senyawa kitin yang sangat kokoh dan resisten terhadap aktivitas enzim. Teknik dan metode spesifik diperlukan untuk mendegradasi dinding sel jamur. Penelitian ini bertujuan untuk menganalisis metode isolasi DNA terbaik dalam melisiskan dinding sel jamur dengan membandingkan beberapa metode isolasi. Metode isolasi yang digunakan adalah DNeasy Plant Mini Kit, kombinasi DNeasy Plant Mini Kit dan pemanasan, serta kombinasi DNeasy Plant Mini Kit dan cara fisik (menggerus sampel dengan menggunakan mortar dan pestle dalam nitrogen cair). Penelitian ini dilakukan pada bulan Maret sampai September 2018 yang bertempat di Laboratorium Mikrobiologi, Laboratorium Bioteknologi dan Genetika, serta Laboratorium Penelitian Terpadu, FMIPA, UNP. Data dianalisis secara kualitatif dengan elektroforesis gel agarose dan secara kuantitatif dengan menghitung nilai kemurnian dan konsentrasi DNA menggunakan Nanodrop. Hasil penelitian menunjukkan bahwa metode kombinasi DNeasy Plant Mini Kit dengan cara fisik memberikan hasil isolasi DNA dengan konsentrasi yang tertinggi yaitu 2,4 μg/ml dengan nilai kemurnian 1,857. Pada metode DNeasy Plant Mini Kit dan metode kombinasi DNeasy Plant Mini Kit dengan pemanasan, konsentrasi DNA yang diperoleh berturut-turut 1,211 µg/ml dan 0,933 µg/ml dengan nilai kemurnian 1,728 dan 1,708. Hasil uji elektroforesis menunjukkan adanya pita DNA berupa garis tipis pada sampel yang diisolasi dengan kombinasi metode kit dan cara fisik, sedangkan pada kedua sampel lainnya tidak ditemukan adanya pita DNA. Dapat disimpulkan metode isolasi DNA yang dapat dijadikan rujukan untuk mendegradasi dinding sel jamur Trichoderma adalah metode kombinasi kit dengan nitrogen cair dengan catatan dibutuhkan penambahan kadar isolat yang digunakan.Kata Kunci: Isolasi DNA, Dinding sel, Trichoderma

Development of Spiritual Nuances Modules in form of Comic Strip on Human Reproductive System Material for Student Grade XI Riri Apriyanti; Lufri Lufri; Dwi Hilda Putri
Bioeducation Journal Vol 2 No 2 (2018): Bioeducation Journal
Publisher : Universitas Negeri Padang Address: Biology Education Study Program Faculty Mathematics and Natural Science (FMIPA) Jl. Prof. Dr. Hamka Air Tawar Barat, Padang-West Sumatera-Indonesia Telp. +62751-7057420 - Fax.+62751-7058772 - Ph. +6281363229286

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bioedu.v2i2.12

The purpose of this research is to develop a module of spiritual nuance in the form of comic strips on reproduction material for students of class XI SMA / MA valid and practical. This research uses 3 of 4 stages 4D models that is define, design and develop. The developed product will be validated by 3 validators including 2 lecturers of biology and 1 biology teacher of SMAN 4 Padang, while for practicality will be done by 32 students of SMAN 4 Padang. The data of this study include the primary data obtained from the questionnaire filled directly by the subject of research. Based on the results of the study obtained the validity of 91.30% with very valid criteria, practicality by teachers 86.68% practical criteria and practicality by students 88.56% with practical criteria.

Development Of Spiritual Special Modules In Form Competition Strip On Material Human Reproduction System For Student Class XI SMA/MA Riri Apriyanti; Lufri Lufri; Dwi Hilda Putri
Bioeducation Journal Vol 1 No 2 (2017): Bioeducation Journal
Publisher : Universitas Negeri Padang Address: Biology Education Study Program Faculty Mathematics and Natural Science (FMIPA) Jl. Prof. Dr. Hamka Air Tawar Barat, Padang-West Sumatera-Indonesia Telp. +62751-7057420 - Fax.+62751-7058772 - Ph. +6281363229286

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bioedu.v1i2.54

The Development Of Modules Based On Problem Solving with Mind Map About Blood Circulation System Material for Students Class VIII Junior High School Salmi Halen; Lufri Lufri; Dwi Hilda Putri
Bioeducation Journal Vol 3 No 1 (2019): Bioeducation Journal
Publisher : Universitas Negeri Padang Address: Biology Education Study Program Faculty Mathematics and Natural Science (FMIPA) Jl. Prof. Dr. Hamka Air Tawar Barat, Padang-West Sumatera-Indonesia Telp. +62751-7057420 - Fax.+62751-7058772 - Ph. +6281363229286

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bioedu.v3i1.119

The implementation of the 2013 Curriculum is to create a creative and innovative person. One of the right approaches is Problem Solving. Circulatory system material is the material that suits the problem solving approach. Less varied teaching materials will make the learning process becomes monotonous so that learners are not interested to learn. Therefore, a research aimed to produce problem-based solving module with mind map of circulatory system material for students of class VIII SMP.This research is a development research using development procedure from Plomp with three stages: initial investigation stage, development stage or prototype, and assessment phase. The object of this research is problem solving module minded mind map, and the subject of research is teachers and learners SMPN 15 Padang.Validity results obtained an average of 86.46% with valid criteria. The results of small group practice test (small group) obtained an average of 100% very practical category. Modular practicality test obtained an average result of 83.33% with practical category, while learners of 79.32% with practical category. It can be concluded that problem solving module with mind map about circulation system material for students of class VIII SMP has been valid and practical.

Meta-Analisis: Efektifitas pemberian pakan buatan untuk meningkatkan laju pertumbuhan dan kualitas reproduksi spesies Monopterus albus Tomi Apra Santosa; Rani Dwi Suci Hd Putri; Ramadhan Sumarmin; Dwi Hilda Putri; Abdul Razak
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p11

The use of artificial feed for fish farming has been widely used at this time, however, it is not yet known the significant effect of artificial feed on the growth and reproduction rate of Monopterus albus. This study aims to determine the effectiveness of artificial feeding on the growth and reproduction rate of Monopterus albus species. This research is meta-analysis research. The data source comes from a search of 18 national and international articles published in 2010-2020 related to feeding the Monopterus albus species. Data obtained from google scholar database, DOAJ, ScienceDirect, sagejournal, Springer, and IEEE. The data analysis technique is a qualitative descriptive analysis with JASP software. The results showed that 35% of artificial feeding affected the growth of Monopterus albus and 28.5% affected the reproductive rate with an effect size of 1.2. This shows that artificial feeding has an effect of 0.334 in increasing the growth and reproductive quality of Monopterus albus.

KUALITAS JAJANAN ANAK SEKOLAH DASAR SECARA MIKROBIOLOGI DI KECAMATAN KOTO TANGAH PADANG SUMATERA BARAT Rahmawita Rahmawita; Dwi Hilda Putri; Linda Advinda
Biomedika Vol 10, No 2 (2018): Biomedika Agustus 2018
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v10i2.7020

ABSTRAKPangan jajanan merupakan sarana bagi anak-anak dalam pemenuhan kebutuhan kecukupan gizi, tetapi juga berisiko menimbulkan keracunan jika tidak higienis. Menurut Undang–Undang RI nomor 18 tahun 2012 tentang pangan menyatakan bahwa keamanan pangan adalah usaha mencegah pangan dari kontaminasi biologis, kimia dan fisik. Laporan kasus kejadian luar biasa (KLB) yang dikeluarkan oleh BPOM tahun 2016 menyatakan bahwa 16,35% keracunan makanan di Indonesia berasal dari pangan jajanan di sekolah dan sebanyak 42,14% berasal dari jajanan yang dibuat dari pangan rumah tangga. Tujuan penelitian ini adalah Untuk menguji kualitas jajanan anak sekolah dasar secara mikrobiologi di Kecamatan Koto Tangah Padang. Penelitian ini bersifat deskriptif untuk mengidentifikasi mikroba patogen dalam jajanan yang di jual pada 19 Kantin Sekolah Dasar pada bulan Maret hingga April 2018. Total sampel yang dapat disampling sebanyak 49 sampel. Pengambilan sampel dilakukan pagi hari hingga menjelang jam istirahat siang. Sampel yang disampling dari kantin dibagi dalam tiga bentuk yaitu jajanan kering, jajanan basah dan minuman yang diberi es batu. Hasil penelitian dengan ditemukannya S.aureus pengkontaminasi pada 1 sampel (2,04%) dari jajanan kering dan E. coli sebanyak 3 sampel (6,12%) dari minuman yang diberi es batu, dan bakteri jenis lain sebanyak 91.82 %.Kata kunci: Kualitas Mikrobiologi, Jajanan Anak Sekolah, Mikroba Patogen Pengkontaminan. ABSTRACTSchool Children Food (SCF) played an important role in providing nutrient intake of children nutritional needs, in the other hand, it may has a risk of poisioning if unhygienic. According to the Law of Republic Indonesia number 18 of 2012 on food states that food security is an attempt to prevent food from biological, chemical and physical contamination. Report on Outbreaks issued by BPOM in 2016 states that 16.35% of food poisoning in Indonesia caused by contaminated SCF, and as much as 42.14% caused by contaminated of household SCF. The aim of this study is to test the microbiological quality of Elementay SCF in Sub-district Koto Tangah Padang. This descriptive research would like to identify pathogenic microbial in SCF which sold at 19 elementary school canteen during periode of time March to April 2018. The total samples are 49. Time of sampling conducted from morning until right before lunchtime. The samples categorized into three forms which are dried, wet and ice-cubed drinks. The result showed that one sample (2.04%) of dried SCF was contaminated by S. aureus, three samples (6.12%) of Iced-cube drinks was contaminated by E.coli and the rest (91.82%) was contaminated by other bacteria. Keyword: Microbiological Quality, School Children Food, Pathogenic Bacteria.

KOLONISASI BAKTERI ENDOFIT PADA AKAR TUMBUHAN ANDALEH (Morus macroura Miq.) Sisri Yandila; Dwi Hilda Putri; Mades Fifendy
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Endophytic bacteria are bacteria living in plant tissues and symbiotic mutualism with their host. The excess production of bioactive substances from endophytic microorganisms among others, microorganisms are easy to grow, have shorter life cycles than plants and can produce large amounts of bioactive compounds. Endophytic bacteria found in plants generally originate from the roots and then spread through xylem tissue. Endophytic bacteria that live within the plant tissue for a certain period and are able to live by forming colonies in plant tissue without harming the host. Endophytic bacteria can be isolated from sterile plant tissue surfaces or extracted from inner plant tissue. The purpose of this study was to determine the type of bacterial isolate of edofit found in the root of Andaleh plant. The method used to isolate endophytic bacteria from the root plant Andaleh is streak plate method. Identification of bacteria macroscopically and microscopically. The results obtained were 16 isolates of endophytic bacteria from the roots of Andaleh plant seen from different colony forms. Identify microscopically with Gram staining

Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul AERMA HASTUTY Afionita, Santi Ahmad Wibisana, Ahmad Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Andri Damayanti, Ndaru Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Az-Zahra, Fauziah Azwir Anhar Cania Dewi, Rahmawitra Cindy Pramila Darussyamsu, Rahmawati Dezi Handayani Dezi Handayani dina vaniana Dinda Sahara Djoelvinanda, Habibullah Edwin Edwin Efliani Efliani Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fahra Fahra Fahra, Fahra Fatma Rahmadhani Febri Doni Febrina Anggiastanti Feby Yeriska Fevria, Resti Fira Safitri Fransisco, Sandi Ghiffari, Muhammad Gustina Indriati Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Larasati Arum Utami Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Mantoviana, Tiffany Marissa Angelina Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini, Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhtar Mukhtar Mulia Mulia Mulia mutia anika Mutia Anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Nisa Afifah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nurfadillatun Nisa Wijaya Nurfatihah Z, Zahara Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Prima, Rika Putri Rachma Auliya Putri, Cici Adelia Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal safitri, fira Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Sari, Rinti Mutiara Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Tomi Apra Santosa Tomi Apra Santosa Santosa Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda Wahyuni Wahyuni Wahyuni, Resma Widya Ruchi Wirda Taufik Y Yusrizal Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri

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