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All Journal Jurnal Biologi Udayana Bioscience Bioeducation Journal Jurnal Ilmu Dasar AL KAUNIYAH Jurnal Biotek Medisiana Indonesia Althea Medical Journal Biota Biomedika BIO-SITE |BIOLOGI Sains Terapan Jurnal Penelitian dan Pembelajaran IPA Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Jurnal Agrotek Tropika Bio-Lectura Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Ilmu Kefarmasian Indonesia AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Majalah Kesehatan Pharmamedika Pelita Eksakta Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi IJECA (International Journal of Education and Curriculum Application) Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Biosense Halal Research Journal TROPICAL GENETICS Scientific Journal MASALIQ: Jurnal Pendidikan dan Sains Tropical Genetics Jurnal Serambi Biologi Berita Biologi
Dwi Hilda Putri
Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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OPTIMIZATION OF STARTER IN PRODUCING ANTIBACTERIAL COMPUNDS ISOLATE B.J.T A-6 FROM ANDALEH PLANT (Morus macroura Miq.) Febrina Anggiastanti; Dwi Hilda Putri
Serambi Biologi Vol 5, No 1 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5691RF00

Abstract

Antibacterial resistance has become a serious problem in the health sector, increasing cases of bacterial resistance to antibacterial agents have encouraged researchers to develop and find new bioactive compounds. One source of new bioactive compounds developed is by utilizing endophyte bacteria. B.J.T A-6 isolate is Andaleh endophyte bacteria which has antibacterial activity. This study aims to determine the concentration of the best fermentation starter in producing antibacterial compounds by ATB 10-6 isolates. The B.J.T A-6 isolate in producing the best antibacterial compound was 24 hours fermentation with a starter concentration of 2.5% at 38 hours observation.
Optimization of Andalas Leaf Tissue Surface Sterilization (Morus macroura Miq.) with NaOCl For Endophytic Microbial Isolation Nur Helmi; Dwi Hilda Putri
Serambi Biologi Vol 6, No 1 (2021): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/11245RF00

Abstract

Morus macroura Miq.atau dikenal dengan nama Andalas memiliki senyawa bioaktif. Salah satu cara untuk mendapatkan senyawa aktif dari tumbuhan Andalas adalah dengan mengisolasi mikroba endofit. Adanya kontaminasi merupakan kendala pada proses isolasi mikroba ini. Optimasi sterilisasi permukaan jaringan merupakan langkah awal yang sangat penting dalam mengisolasi mikroba endofit. Salah satu bahan sterilan yang umum digunakan adalah menggunakan natrium hipoklorit (NaOCl).Tujuan penelitian ini adalah untuk mengetahui konsentrasi dan waktu optimum untuk sterilisasi permukaan jaringan daun Andalas (Morus macroura Miq.) yang menggunakan natrium hipoklorit (NaOCl). Optimasi sterilisasi permukaan daun Andalas menggunakan larutan NaOCl dengan konsentrasi (0,25% dan 0,50%), dan waktu sterilisasi yang akan digunakan (0,5, 1, 1,5, dan 2 menit). Analasis mikroba kontaminan yaitu dengan bakteri ditumbuhkan pada medium Nutrient Agar (NA) dan jamur pada medium Potato Dextrosa Agar (PDA).                            Hasil penelitian menunjukkan bahwa optimasi konsentrasi sterilisasi permukaan daun Andalas untuk isolasi bakteri endofit diperoleh hasil konsentrasi optimum 0,25% dan waktu optimum selama 0,5 menit, dan konsentrasi 0,50% dengan waktu optimum selama 1 menit, sedangkan untuk jamur endofit pada daun Andalas diperoleh hasil konsentrasi 0,25% dan 0,50% dengan waktu optimum selama 0,5 menit.
Primer Design For Identification Of Beta-Carotene Encoding Genes In Cassava mutia anika; Dwi Hilda Putri; Wahyuni Wahyuni
Serambi Biologi Vol 4 (2019): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5320RF00

Abstract

Primers are oligonucleotides which have an important role in the PCR process. To get the primer can be done by doing a primary design. Beta-carotene encoding genes are genes that play a role in the formation of beta-carotene in carotenoid biosynthetic pathways in several plants including cassava. This study aims to obtain primers who can identify beta-carotene encoding genes in cassava plants. Primers are designed using primary 3 plus software online. The results obtained were five specific primers, namely PSY, CRTISO, LCY α, LCY β, and BCH primers which could identify beta-carotene coding genes such as PSY, CRTISO, LCY α, LCY β, and BCH on cassava. Primers designed according to the criteria for primary length, GC content and temperature melting for beta-carotene encoding genes in cassava.
Test the Quality of Petai Seed Flavor (Parkia speciosa Hassk.) Fermentation Results Using Tempe Yeast Sity Sarroh; Dezi Handayani; Dwi Hilda Putri
Serambi Biologi Vol 4 (2019): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5683RF00

Abstract

Abstract. Petai plants (Parkia speciosa Hassk.) Are thought to originate from Malaysia. The most important part of the petai plant to use is the seeds. Petai seeds contain hexathionine, tetrathiane, trithiolane, pentathiopane, pentathiocane, and tetrathiepane which cause the petai to emit a smell of smell. One way to reduce the smell of smell in petai is to do fermentation. Benefits of fermented food products are for preservation, enhancing taste, and for producing new products. This study aims to find out whether from color, texture, aroma can be accepted by the community. This research was conducted at the Research Laboratory and Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences, Padang State University from October 2018 to March 2019. The data of this study were obtained from questionnaires tested using hedonic tests (preferences) and presented in the form of percentages. The results of this study showed that for color 74% while the criteria did not like 64%, for texture as much as 72% of those who liked and who did not like 52%, and for aroma 58%, while those who did not like 46%, for flavor taste of tempai petai seeds 66% while those who don't like 32%. The physical characteristics of tempeh are in accordance with the criteria of SNI 3144: 2009, namely: having a normal odor (typical tempe), normal color (white), compact texture, and normal taste (typical taste of tempe and not foreign).Keywords: Petai, Organoleptic,  Fermentation
16S rRNA Gene Amplification Of Endophytic Bacteria Which Produces Antimicrobial Compounds Rahmat Afif; Dwi Hilda Putri
Serambi Biologi Vol 4 (2019): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5328RF00

Abstract

Cases of bacterial resistance to antibiotics are a serious problem in the world of health. Exploration to find new sources of antibiotics needs to be done, one of them is by utilizing endophytic bacteria Andaleh. The purpose of this study was to amplify the 16S rRNA coding gene, three isolates of Andaleh endophytic bacteria producing antimicrobial compounds with ATB A4.1, B.J.T.A.2.1, B.J.T.A.3 isolate code. This research is a descriptive research, carried out from November 2017 to June 2018 at the Research Laboratory of the Biology Department FMIPA UNP. The results showed that the amplification of the 16S rRNA gene from the three isolates succeeded in producing a single DNA band located between DNA bands 2027bp and 564 bp from the marker λ HindIII.
ANALYSIS INTERNAL PRIMER OF GENES 16S RRNA ENDOPHYTIC BACTERIA PRODUCING COMPOUND ANTIMICROBIAL FOR SEQUENCING dina vaniana; Dwi Hilda Putri
Serambi Biologi Vol 4 (2019): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5330RF00

Abstract

Endophytic bacteria can produce active compounds that function as antitumor, antioxidant and antimicrobial. The steps taken can be done for the use of endophytic bacteria is identification of bacteria. Endophytic bacteria have a 16S rRNA gene. The most widely used technique for identification is DNA sequencing. For the sequencing process it takes a primer that can read 1500 bp sequences. The purpose of this study was to obtain primers who could read the internal area of the 16S rRNA gene and be able to be used in the sequencing process. This research uses BioEdit software and Primer Designer. The isolate used as a DNA template in the sequencing process is B.J.T.A.2.1. DNA template were amplified using the PCR method. The results of PCR products were analyzed using 1% agarose gel electrophoresis. The sequencing process is carried out with NIF (forward) and NIR (reverse) primers. The results showed that the primer could recognize the 16S rRNA internal region of the antimicrobial compound endophytic bacteria. Both primers are able to start the sequencing process and produce a sequence of 1130 bp. However, good sequence readings were only ± 100 bp due to contamination of DNA template.
THE ANTIMICROBIAL ACTIVITY OF DMSO AS A NATURAL EXTRACT SOLVENT Miftahul Rahmi; Dwi Hilda Putri
Serambi Biologi Vol 5, No 2 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5909RF00

Abstract

Abstrak. Salah satu penyebab meningkatnya kasus infeksi karena adanya peningkatan jumlah kuman yang resisten terhadap agen antimikroba. Isolat B.J.T.A.2.1 merupakan bakteri endofit dari tanaman Andalas (Morus macroura Miq.) yang diketahui mampu menghasilkan senyawa aktif antimikroba. Penelitian ini bertujuan untuk mengetahui pengaruh pelarut DMSO yang merupakan pelarut dari  ekstrak etanol produk fermentasi terhadap hasil uji. Uji aktivitas pelarut dilakukan dengan metode difusi kertas cakram. Hasil penelitian menunjukkan bahwa tidak ada pengaruh pelarut DMSO terhadap hasil uji. Kata kunci: ekstrak etanol produk fermentasi, senyawa antimikroba, DMSO.  Abstract. One of the causes of increased cases of infection is the increase in the number of germs that are resistant to antimicrobial agents. Isolate B.J.T.A.2.1 is an endophytic bacterium from Andalas (Morus macroura Miq.) Plant which is known to produce antimicrobial active compounds. This study aims to determine the effect of DMSO solvent which is a solvent from ethanol extract of fermented products on the test results. The solvent activity test was carried out by paper diffusion method. The results showed that there was no effect of DMSO solvent on the test results. Keywords: ethanol extract of fermented products, antimicrobial compounds, DMSO.
PRECISION ENUMERATION OF THE NUMBER OF BACTERIAL CELLS WITH THE SPREAD PLATE METHOD USING DILUTION Elsa Alfiyanti; Dwi Hilda Putri
Serambi Biologi Vol 5, No 1 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5768RF00

Abstract

Bakteri endofit isolat B.J.T.A.2.1 merupakan isolat yang menghasilkan senyawa antimikroba yang baik. Tetapi, saat ini belum diketahui persisi jumlah sel bakteri tersebut. Penelitian ini bertujuan untuk mengetahui dan mendapatkan persisi jumlah sel bakteri. Penelitian ini dilakukan dengan cara enumerasi melalui perhitungan menggunakan pengenceran. Metode penanaman yang digunakan adalah metode sebar (spread plate). Metode penelitian yang digunakan adalah deskriptif yaitu melakukan analisis data dan disajikan dalam bentuk grafik dari jumlah sel bakteri. Parameter analisis adalah jumlah sel bakteri. Hasil penelitian menunjukkan bahwa persisi jumlah sel bakteri adalah -0,8 hingga +1,2. Zona biru pada grafik adalah persisi (ketepatan) jumlah sel bakteri.kata kunci: persisi, jumlah sel bakteri
Optimization of Medium Fermentation for Production of Antimicrobial Compounds by Endofit Bacteria Andalas Plant (Morus macroura Miq.) B.J.T.A-6 Isolate Nada Nafion; Dwi Hilda Putri; Irdawati Irdawati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (594.109 KB) | DOI: 10.24036/0201931102865-0-00

Abstract

Cases of bacterial resistance to antibiotics are discussed with serious problems in the world of health. New antimicrobial compounds are needed which are more effective in treating infectious diseases. Isolate B.J.T.A-6 is an endophytic bacteria from Andalas plants (Morus macroura Miq.) which is known to be able to produce antimicrobial active compounds. Antimicrobial compounds can be produced by growing them on fermented media. The purpose of this study was to optimize the medium fermentation of Andalas endophytic bacteria of B.J.T.A-6  isolates in producing antimicrobial compounds. While medium fermentation is Nutrient Broth (NB), Muller Hinton (MH), and Luria Bertani Broth (LB). Antimicrobial activity tests were carried out by means of diffusion. The parameters used were the diameter of the inhibition zone against Staphylococcus aureus around the disc paper. The profile of medium fermentation optimization was analyzed statistically. The results showed that B.J.T.A-6  isolates could inhibit the highest growth of S. aureus by using LB fermentation medium. 
Analysis Of ZDS and LCYb Enzyme Coding Gene Related To Beta Carotene Bioshynthesis in Cassava (Manihot esculenta Crantz) using Reverse transcription PCR (RT-PCR) Jihan Rezi Okanti; Dwi Hilda Putri; Ahmad Fathoni
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (781.038 KB) | DOI: 10.24036/0202041106599-0-00

Abstract

This study aims to determine variations in the expression of ZDS and LCYb enzymes encoding several types of yellow tubers. Cassava used, namely Carvita 25, Nangka and Mentega 2 (yellow bulb) and Menti (white bulb) were used as controls. The target enzyme coding genes (ZDS and LCYb) in cassava were identified in an online database, Phytozome using the BLAST method. Design and primary analysis for the target genes was carried out using online software, OligoAnalyzer and PCR. The expression of the target enzyme coding gene was analyzed using the Reverse Transcription PCR (RT-PCR) method. The BLAST results in the cassava genome in the Phytozome database showed that the enzymes ZDS and LCYb in cassava were encoded by two genes. The primary optimization results showed the primary annealing temperature for the LCYb enzyme coding gene was 55 ° C. The ZDS primers were not amplified after several replications, so that only the LCYb enzyme coding gene was selected for qualitative gene expression analysis using RT-PCR. A positive control in the analysis of gene expression is the housekeeping gene, PP2A. Electrophoresis results of PCR products (RT-PCR) showed negative results (no DNA bands were detected) in all samples from both yellow and white tuber samples and its housekeeping gene. This is possible from the low quality of RNA used in cDNA synthesis.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul ADE ANDRIANI AERMA HASTUTY Afandi, Echa Azkia Afionita, Santi Afroza, Faiza Ahmad Wibisana, Ahmad Albar, Rahmat Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Andri Damayanti, Ndaru Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Astrid Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Ayuningtyas, Maulia Indah Az-Zahra, Fauziah Azwir Anhar Benny Alexander Maisa Berlinda Paradisa, Yashanti Cania Dewi, Rahmawitra Chatri, Moralita Chatri Cindy Pramila Darussyamsu, Rahmawati Des M Dezi Handayani Dezi Handayani Dina Sukma dina vaniana Dinda Sahara Djoelvinanda, Habibullah Edwin Edwin Efliani Efliani Eka Nuraini, Fauzi Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fadilaturahmah Fadilaturahmah Fahra Fahra Fahra, Fahra Farras, Fadhila Annisa Fatma Rahmadhani Febri Doni Febrianti, Liza Febrina Anggiastanti Feby Yeriska Fevria, Resti Fhuji Winardi Filza Yulina Ade Fira Safitri Fransisco, Sandi Fronica, Imelda Fuadiyah, Sa’diatul Ghiffari, Muhammad Gustina Indriati Handayani , Dezi Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal IWAN SASKIAWAN Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Kheniva Diah Anggita Kiki Amelia, Kiki Larasati Arum Utami Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Mantoviana, Tiffany Mardhiyah Nazri, Laila Marissa Angelina Marten, Threo Wanda Mellani Rachma Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini, Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhlis Mukhlis Mukhtar Mukhtar Mulia Mulia Mulia mutia anika Mutia Anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Nazri, Laila Mardhiyah Nisa Afifah Novitasari, Yuliana Diyah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nuraini, Fauzi Eka Nurfadillatun Nisa Wijaya Nurfatihah Z, Zahara Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Pratama, Sandi Fransisco Prima, Rika Putri Qalbina Putri Rachma Auliya Putri, Aulia Devani Putri, Cici Adelia Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal safitri, fira Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Sari, Rinti Mutiara Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh SOLICHATUN SOLICHATUN Solusia, Carbiriena Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Taufiqa, Zuhrah Tomi Apra Santosa Tomi Apra Santosa Santosa Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda wahyuni wahyuni Wahyuni Wahyuni Wahyuni, Resma Widya Ruchi Wilna Sari Winardi, Fhuji Wirda Taufik Wulandari, Tesya Yannita, Defni Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri