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All Journal Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Journal of Agromedicine and Medical Sciences Mutiara Medika: Jurnal Kedokteran dan Kesehatan MNJ (Malang Neurology Journal) Majalah Kedokteran Sriwijaya JPSriwijaya Biomedical Journal of Indonesia Jurnal Kesehatan Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Surya Medika Jurnal Penelitian Pendidikan IPA (JPPIPA) Syntax Literate: Jurnal Ilmiah Indonesia Acta Biochimica Indonesiana Oceana Biomedicina Journal SRIWIJAYA JOURNAL OF MEDICINE Majalah Patologi Indonesia Bioscientia Medicina : Journal of Biomedicine and Translational Research Medica Hospitalia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Best Journal (Biology Education, Sains and Technology) Jurnal Ners Indonesian Journal of Global Health research Jurnal Pengabdian Masyarakat: Humanity and Medicine Bioscientia Medicina : Journal of Biomedicine and Translational Research Conferences of Medical Sciences Dies Natalis Jurnal Keperawatan Molekul: Jurnal Ilmiah Kimia
Zen Hafy
Universitas Sriwijaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Hubungan metode deparafinisasi dengan kuantitas dan kualitas ekstrak dna hasil isolasi dari sampel arsip jaringan dalam blok parafin terfiksasi formalin Ray Suga Aulia; Zen Hafy; Subandrate Subandrate
JURNAL KEDOKTERAN DAN KESEHATAN Vol 4, No 1 (2017)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kuantitas dan kualitas hasil ekstraksi DNA dari jaringan FFPE sangat tergantung dari proses awal yang harus dilakukan pada sampel sebelum masuk kedalam tahapan ekstraksi DNA. Proses awal yang sangat menentukan keberhasilan ekstraksi DNA ini adalah proses deparafinisasi, yaitu proses penghilangan parafin jaringan dari parafin. Penelitian ini bertujuan untuk membandingkan kualitas DNA yang diambil dari sampel arsip FFPE (Formalin-fixed paraffin-embedded tissue) dengan metode deparafinisasi menggunakan xylene dan mineral oil di RSUP Dr. Mohammad Hoesin Palembang. Sebanyak 16 sampel arsip FFPE diambil dari Laboratorium Patologi Anatomi RSUP dr. Mohammad Hoesin Palembang. Seluruh sampel kemudian  dibagi menjadi 2 bagian sama rata kemudian dilakukan proses deparafinisasi menggunakan xylene dan mineral oil. DNA yang telah diisolasi akan diuji kuantitasnya menggunakan spektrofotometer dengan panjang gelombang 260nm.  Uji kualitas akan dilakukan dengan menghitung OD Ratio 260nm:280nm. Selain itu DNA juga akan diamplifikasi lalu dilakukan visualisasi dengan sinar UV. Konsentrasi yang didapatkan kemudian diuji dengan uji Wilcoxon dan menunjukkan adanya hubungan signifikan antara metode deparafinisasi dan konsentrasi yang didapat (P=0.036). Primer beta-actin (524bp)tidak ditemukan potongan gen yang diinginkan setelah elektroforesis, kemudian diuji dengan primer Mitin (142bp) menunjukan potongan gen yang diinginkan pada saat elektroforesis dan visualisasi UV.Terdapat hubungan yang signifikan antara metode deparafinisasi FFPE dengan kuantitas dan kualitas DNA yang didapat.
Hubungan Kadar CA-125 Dengan Subtipe Epitel Tumor Ganas Ovarium Pada Penderita Yang Dirawat Di RSUP Dr. Mohammad Hoesin Palembang Tahun 2013-2016 Rikka Wijaya; Krisna Murti; Zen Hafy
Majalah Kedokteran Sriwijaya Vol 49, No 4 (2017): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/mks.v49i4.8526

Abstract

Karsinoma ovarium tipe epitel merupakan 75% dari semua kasus tumor ovarium. Di dunia kanker ovarium merupakan keganasan ginekologi peringkat ketiga dari keganasan saluran genital pada wanita. Tumor marker keganasan ovarium tipe epitel adalah CA-125 yang ditemukan meningkat pada hampir semua kasus tumor ovarium (80%). Diduga terdapat perbedaan peningkatan kadar CA-125 pada berbagai subtipe epitel tumor ganas ovarium yaitu serosum (37,5%), endometrioid (50%), musinosum (62,5%), undifferentiated (84,3%) dan clear cell (100%). Tujuan penelitian ini untuk mengetahui hubungan kadar CA-125 dengan subtipe tumor ganas epitel ovarium pada penderita yang dirawat di RSUP Mohammad Hoesin Palembang. Penelitian ini menggunakan studi observasional analitik dengan desain cross sectional. Populasi penelitian adalah semua penderita subtipe epitel tumor ganas ovarium yang melakukan pemeriksaan kadar CA-125 di Bagian Patologi Anatomi RSMH Palembang sejak Januari 2013 sampai September 2016. Dari 61 sampel berbagai subtipe epitel tumor ganas ovarium yang didominasi subtipe serosum (32,8%), diikuti subtipe musinosum (26,2%), disusul oleh subtipe endometrioid (19.7%), clear cell (14,8%), borderline musinosum (4,9%) dan subitpe brenner (1.6%). Kadar CA-125 meningkat dijumpai delapan kali lipat (88,5%) lebih banyak dibanding subjek dengan kadar normal (11,5%). Hasil uji one-way ANOVA menunjukkan tidak terdapat perbedaan antara kadar CA-125 dengan masing-masing subtipe epitel tumor ganas ovarium. Hasil uji statistik Chi-square menunjukkan bahwa tidak terdapat hubungan yang bermakna antara kadar CA-125 dengan subtipe tumor ganas epitel ovarium. Tidak terdapat hubungan yang bermakna antara kadar CA-125 dengan subtipe tumor ganas epitel ovarium di RSMH  Palembang.
OPTIMALISASI PENCEGAHAN COVID-19 PADA PENDERITA DIABETES MELITUS MELALUI EDUKASI DAN PELATIHAN FORMULASI MINUMAN KUNYIT, SUSU, KAYU MANIS (KUSUKA) UNTUK MENINGKATKAN SISTEM IMUN Laida Neti Mulyani; Veny Larasati; Dwi Handayani; Zen Hafy
Jurnal Pengabdian Sriwijaya Vol 8, No 4 (2020)
Publisher : Lembaga Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37061/jps.v8i4.13196

Abstract

COVID-19 secara dominan mempengaruhi jenis kelamin pria dan usia lanjut dengan komorbiditas, terutama penyakit paru-paru, jantung, arteri hipertensi (AH) dan diabetes mellitus (DM). Penderita DM sangat riskan jika terinveksi COVID-19. Pada kegiatan pengabdian pada masyarakat kali ini dilakukan  penyuluhan dan pelatihan untuk optimalisasi Pencegahan Covid-19 Pada Penderita Diabetes Melitus Melalui Edukasi dan Pelatihan Formulasi Minuman Kesehatan Kunyit, Susu, Kayu Manis (Kusuka) Untuk Meningkatkan Sistem Imun. Metode pelaksanaan kegiatan meliputi Metode pelaksanaan pada kegiatan kali ini mencakup tahapan persiapan, pelaksanaan kegiatan dan dan evaluasi pelaksanaan kegiatan. Berdasarkan Hasil pelaksanaan kegiatan menunjukkan bahwa persiapan kegiatan berjalan dengan baik. Diskusi yang dinamis antara pemateri dan warga ketika pelaksanaan kegitan menunjukk antusiasme warga yang cukup tinggi serta hasil evaluasi yang diberikan pada warga melalui wawancara menunjukka bahwa warga dapat memahami mengenai pencegahan Covid-19 bagi Penderita Diabetes Melitus dan terampil dalam membuat minuman kesehatan untuk meningkatkan system Imun. Sehingga dapat disimpulkan bahwa melalui kegiatan sosialisasi dan pelatihan dapat meningkatkan wawasan dan pengetahuan warga, serta keterampilan warga dalam formualsi minuman kesehatan dari susu dan rempah untuk meningkatkan sistem imun.
The relationship between the umbilical cord length and it's diameter with the total CD34+ and total nucleated cell (TNC) as a parameter of cord blood selection Meiliza Indriani; Ya’kub Rahadiyanto; Yusuf Effendi; Bayu Winata Putera; Zen Hafy
Acta Biochimica Indonesiana Vol. 1 No. 2 (2018): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v1i2.12

Abstract

Background: The stem cell transplantation successful influenced by the quality of the umbilical cord blood which includes the number of CD34 + and Total Nucleated Cells (TNC). Objectives: The study aims to determine the correlation between umbilical cord length and it’s diameter, with the number of CD34 + and TNC cells as indicators of the quality of cord blood storage feasibility. Several other factors as maternal age, gestation period, and infant birth weight also examined. Methods: Thirty four of umbilical cords from the delivered woman in Dr. Mohammad Hoesin Hospital (RSMH) Palembang were collected from May to June 2018. The length and diameter of the cord were immediately measured after delivery. The evaluated cells were counted with a flow cytometer at Klaster Stem Cell and Tissue Engineering Research Centre (SCTE) IMERI Faculty of Medicine University of Indonesia (FKUI). Results: Spearman correlation test show that there was no correlation between the length and diameter of the umbilical cord, maternal age, gestational period and infant weight, with the number of CD34 + and TNC cells in the cord (p> 0.05). However, the profile analysis indicated that the longer and larger the diameter of the umbilical cord, the higher concentration of the CD34+ and TNC cells. Conclusion: This study suggested that the younger maternal age, older gestational age, and higher infant birth weight, also normal hemoglobin level, tend to increase the number of CD34+ and TNC cells in the cord blood.
N-Acetyl-Glucosamidase as Biomarker for Acute Kidney Injury Aulia Annisa Rizki; Zulkifli Zulkifli; Agustina Br Haloho; Zen Hafy
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 4 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i4.56

Abstract

Background : The estimated inci­dence of Acute Kidney Injury (AKI) is two to three cases per 1,000 patients. Seven percent of hospitalized patients and about two-thirds of patients in intensive care units develop acute kidney injury and the mortal­ity rates range between 25 and 80 percent. Disruption in epithelial brush border of proximal tubular cell causes N-Acetyl-β-D-Glucosaminidase (NAG) to be released to the urine and the amount of enzyme could be directly correlated with tubular disruption. Objective : The aims of this research is to determine the sensitivity and specificity of NAG urine examination as an early biomarker for acute kidney injury. Methods : There’s 66 subjects who met the inclusion criteria. All the subject were checked for the NAG urine level with Cloud Clone kit and creatinine serum were also checked 48 hours after admission. Results : The results showed in the cut-off point of 7.98 Ng/mL, urine NAG has a sensitivity of 68.6% and specificity 77.4%, positive predictive value 77.42%, negative predictive value 68.57 % and accuracy of 72.73% Conclusions : The result shows that urine NAG examination is more sensitive and specific as an early biomarker for Acute Kidney Injury compared to creatinine serum. Key words : Acute Kidney Injury, N-Acetyl-β-D-Glucosaminidase , sensitivity, spesificity
Polymorphism of the Klotho G-395a Gene Promoter with the Incidence of Preeclampsia Desi Arlindia; Mgs. Irsan Saleh; Zen Hafyy; Legiran; Erial Bahar; Kms. Yusuf Effendi; Ferry Yusrizal
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 1 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i1.178

Abstract

A B S T R A C TIntroduction. Preeclampsia is one of the leading causes of maternal and perinatalmorbidity and mortality and is still a disease of theory. Klotho is a new gene, inseveral biological processes in the pathophysiology of preeclampsia that play a rolein regulating endothelial nitric oxide production, angiogenesis, production ofantioxidant enzymes and protection against endothelial dysfunction. The Klotho G-395A genotype AA promoter polymorphism is the cause of hypertension. This studyaims to determine the relationship of the Klotho G-395A promoter polymorphism tothe incidence of preeclampsia. Methods. This study is an analytical study with acase-control design. The research was conducted at Pembina community healthcentre of Palembang and the public hospital of Prabumulih in February - July 2020and involving 50 case group and 50 control group. To determine the genotype andallotype of the Klotho G-395A gene promoter polymorphism, using polymerase chainreaction examination. Result. The results showed that the risk factors for maternalage and maternal gestational age had a significant relationship with the incidence ofpreeclampsia (p-value 0.015; p-value 0.000). There was a significant relationshipbetween the Klotho G-395A genotype GA + AA promoter polymorphism and theincidence of preeclampsia (p-value 0.024; OR = 2.571; 95% CI = 1.122-5.895), whileallotypes in the study sample also had a significant relationship with the incidenceof preeclampsia. preeclampsia (p-value 0.025; OR = 1.978; 95% CI = 1.087-3.599).Conclusion: There is a significant relationship between the Klotho G-395A genepromoter polymorphism and the incidence of preeclampsia.
Gynaecologic History During Adolescence to Predict Endometriosis Earlier Hartati; Dwiana Ocviyanti; Ramzi Amin; Yusuf Effendi; Zen Hafy; Radiyati Umi Partan; Rafika Novianti
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 3 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i3.465

Abstract

Background. Endometriosis is a chronic gynecological disorder that generally affects women of reproductive age which is characterized by implantation of endometrial tissue, both glands and stroma outside the uterus. Research shows that the initial onset of endometriosis patients begins in adolescence. The high prevalence with atypical symptoms leads to long term delay in establishing the appropriate diagnosis. Further developments in the field of early endometriosis screening will greatly assist clinicians, especially general practitioners in areas with limited health facilities, in early detection of endometriosis. Methods. Using the electronic databases, comprehensive literature searches were conducted with the specific keywords. Full-text manuscripts published were reviewed for relevancy and importantly, reference lists were cross-checked for additional relevant studies. Results. Dysmenorrhea, chronic pelvic pain, dyspareunia, dysuria, dyschezia, and glycoprotein were found dominantly in all analyzed literature. The occurrence of endometriosis affects the functioning of women in multiple aspects of life. Endometriosis places a significant burden on teens and adult women, their families, and society as a whole. Conclusion. Combination of structured past history taking, and several biomarker level might be useful as a screening tool that easy to use and cost effective, as early diagnosis is expected to prevent further progression of endometriosis especially in adolescence.
Neutrophil Extracellular Traps and Its Correlation with Several Pathological Conditions: Prosperities and Deleterious Implications Phey Liana; Krisna Murti; Zen Hafy; Iche Andriyani Liberty; Tungki Pratama Umar
Molecular and Cellular Biomedical Sciences Vol 6, No 1 (2022)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v6i1.224

Abstract

Neutrophil extracellular traps (NETs) are immune components found in a variety of pathological states. It has been shown to have either beneficial or harmful implications, depending on how it is controlled and has been particularly observed in three major scenarios: infection, autoimmune disease, and cancer. In this article, we compiled some of the roles of NETs in pathological conditions, as well as the benefits of targeting them for improved patient outcomes. The role of NETs were primarily positive in infectious disease, whether caused by bacteria, virus, or fungal infection. In non-infectious inflammatory scenarios, on the other hand, it's the complete opposite, with the effects being mainly deleterious and even worse than the original disease states. Targeting NETs directly or indirectly may help to prevent complications and improve patient outcomes. A plethora of compounds, including immunomodulators, anti-thrombosis, nicotinamide adenine dinucleotide phosphate (NADPH)/reactive oxygen species (ROS) inhibitors, nuclease, and other compounds, may be used to accomplish the therapeutic goals.Keywords: autoimmune disease, cancer, DNase, infection, neutrophil extracellular traps
N-Acetyl-Glucosamidase as Biomarker for Acute Kidney Injury Aulia Annisa Rizki; Zulkifli Zulkifli; Agustina Br Haloho; Zen Hafy
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 4 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i4.56

Abstract

Background : The estimated inci­dence of Acute Kidney Injury (AKI) is two to three cases per 1,000 patients. Seven percent of hospitalized patients and about two-thirds of patients in intensive care units develop acute kidney injury and the mortal­ity rates range between 25 and 80 percent. Disruption in epithelial brush border of proximal tubular cell causes N-Acetyl-β-D-Glucosaminidase (NAG) to be released to the urine and the amount of enzyme could be directly correlated with tubular disruption. Objective : The aims of this research is to determine the sensitivity and specificity of NAG urine examination as an early biomarker for acute kidney injury. Methods : There’s 66 subjects who met the inclusion criteria. All the subject were checked for the NAG urine level with Cloud Clone kit and creatinine serum were also checked 48 hours after admission. Results : The results showed in the cut-off point of 7.98 Ng/mL, urine NAG has a sensitivity of 68.6% and specificity 77.4%, positive predictive value 77.42%, negative predictive value 68.57 % and accuracy of 72.73% Conclusions : The result shows that urine NAG examination is more sensitive and specific as an early biomarker for Acute Kidney Injury compared to creatinine serum. Key words : Acute Kidney Injury, N-Acetyl-β-D-Glucosaminidase , sensitivity, spesificity
Polymorphism of the Klotho G-395a Gene Promoter with the Incidence of Preeclampsia Desi Arlindia; Mgs. Irsan Saleh; Zen Hafyy; Legiran; Erial Bahar; Kms. Yusuf Effendi; Ferry Yusrizal
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 1 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i1.178

Abstract

A B S T R A C TIntroduction. Preeclampsia is one of the leading causes of maternal and perinatalmorbidity and mortality and is still a disease of theory. Klotho is a new gene, inseveral biological processes in the pathophysiology of preeclampsia that play a rolein regulating endothelial nitric oxide production, angiogenesis, production ofantioxidant enzymes and protection against endothelial dysfunction. The Klotho G-395A genotype AA promoter polymorphism is the cause of hypertension. This studyaims to determine the relationship of the Klotho G-395A promoter polymorphism tothe incidence of preeclampsia. Methods. This study is an analytical study with acase-control design. The research was conducted at Pembina community healthcentre of Palembang and the public hospital of Prabumulih in February - July 2020and involving 50 case group and 50 control group. To determine the genotype andallotype of the Klotho G-395A gene promoter polymorphism, using polymerase chainreaction examination. Result. The results showed that the risk factors for maternalage and maternal gestational age had a significant relationship with the incidence ofpreeclampsia (p-value 0.015; p-value 0.000). There was a significant relationshipbetween the Klotho G-395A genotype GA + AA promoter polymorphism and theincidence of preeclampsia (p-value 0.024; OR = 2.571; 95% CI = 1.122-5.895), whileallotypes in the study sample also had a significant relationship with the incidenceof preeclampsia. preeclampsia (p-value 0.025; OR = 1.978; 95% CI = 1.087-3.599).Conclusion: There is a significant relationship between the Klotho G-395A genepromoter polymorphism and the incidence of preeclampsia.
Co-Authors A, Rafdi A, Rafdi Abdullah Fikri Abdullah Sahab Afriyana Siregar Afriyana Siregar Agnes Felicia Lubis Agnes Felicia Lubis Agustian Dwi Putra Agustian Dwi Putra Agustina Br Haloho Ahmad, Zen Amalia Amalia Anisah Nida'ul Haq Argentina, Fiva Ari Wahyuni aryani, Inda astri Aulia Annisa Rizki Bastian Bastian Bayu Winata Putera Calvin Ienawi Chairil Anwar Citra Ananta Avis Citra Dewi Debby Handayati Harahap Desi Arlindia Didit Pramudhito Dwi Handayani Dwi Handayani Dwiana Ocviyanti Dwirini Retno Gunarti Dya Anggraeni Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim, Eddy Mart Efrieni Erial Bahar Erial Bahar Erial Bahar Erial Bahar, Erial Ernawati Sinaga Fachmi Idris Fadilah, Rizka Ferry Yusrizal Ferry Yusrizal Fitri Octaviana Fitri Octaviana Francisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Gita Dwi Prasasty Hadi Nugraha Mustofa Haekal M Handayani, Sri Hartati Harun Hudari Harun Hudari Harun, Yusril Heni Maulani Heni Pujiastuti Henny Sulastri Hermansyah Hermansyah Hu, Owen Iche Andriyani Liberty, Iche Andriyani Indra Franajayakk Inggarsih, Rara Irsan Saleh Jayawarsa, A.A. Ketut Jusak Nugraha Jusuf Fantoni Kemas Ya'kub Rahadiyanto Kemas Ya'kub Rahadiyanto Kemas Ya’kub Rahadiyanto Kemas Yusuf Effendi Kms Yusuf Effendi Kms. Yusuf Effendi Kms. Yusuf Effendi Kms. Yusuf Effendi Kms. Yusuf Effendi Kodrati, Adika Krisna Murti Krisna Murti kurniati, nova Laida Neti Mulyani Larasati, Veny Legiran Legiran Legiran Legiran Lubis, Fadhyl Zuhry M, Haekal M, Haekal M. Irsan Saleh Masagus Irsan Saleh Hasani Maya Eka Apriyanti Meiliza Indriani Mgs. Irsan Saleh Mgs. Irsan Saleh Mohamad Sadikin Muhammad Irsan Saleh Nita Hertati Nova Kurniati Nova Kurniati Nova Kurniati Nova Kurniati Nova Kurniati Nova Lestari Novizar S Ocktariyana, Ocktariyana Oktariana, Desi Pancarani, Muftia Phey Liana Prihantika S., Sabrina Purnamasari, Septi Puspita, Riana Sari Puspita, Riana Sari R, Sentani R, Sentani Radiyati Umi Partan Radiyati Umi Partan, Radiyati Umi Rafdi A Rafika Novianti Rahadiyanto, Kemas Ya'Kub Rahmi Widiyawati Ramadhayanti, Lidya Ramzi Amin, Ramzi Rasyid, Riana Sari Puspita Ray Suga Aulia Ray Suga Aulia Sentani Riana Sari Puspita Riana Sari Puspita Rasyid Rikka Wijaya Rini Nindela Rini Nindela, Rini Rizal Sanif S, Novizar S, Novizar Sabri Prihantika Sabrina Prihantika Saleh, M Irsan Saleh, Mgs. M. Irsan Salutondok, Welly Sari, Nurmalia Purnama selly marisdina Selvy Apriani Sentani R SEPTI WULANDARI Soilia Fertilita Sri Handayani Sri Sulpha Siregar Sri Sulpha Siregar Subandrate Sudarto Sudarto Suly Auline Rusminan Susiwati, Susiwati Syazili Mustofa Syifa Alkaf Tanoerahardjo, Francisca Srioetami Tungki Pratama Umar Venny Larasati Venny Larasati Verli Saniba Wresnindyatsih Ya’kub Rahadiyanto Yudianita Kesuma, Yudianita Yunni Diansari Yusuf Effendi Yusuf Effendi Yusuf Effendi Yusuf Effendi Zen Ahmad Ziske Maritska Zulkarnain Musa