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ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
Evaluation of Mangrove, Acanthus ilicifolius Aqueous Extract on Inhibition of Dengue Virus Replication: In Vitro and Molecular Docking Approach Wiradana, Putu Angga; Wiartini, Ni Wayan Ayu Wiartini; Sucipto, Teguh Hari; Herdiansyah, Mochammad Aqilah; Permatasari, Anak Agung Ayu Putri; Widhiantara, I Gede; Sari, Ni Kadek Yunita Sari; Darmanto, Win; Panjaitan, Novaria Sari Dewi Panjaitan; Kusala, Muhammad Khaliim Jati Kusala
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.71217

Abstract

Dengue virus (DENV) infection is a worldwide health risk, presenting severe symptoms with possibly lethal consequences. The search for effective antiviral drugs against DENV has largely focused on natural products. Mangrove, Acanthus ilicifolius, has been empirically shown to exhibit various biological activities and good safety profiles.  The purpose of this investigation was to examine the antiviral activity of A. ilicifolius aqueous extract against DENV, and the way it functions of action of its bioactive components function was explored utilizing an in vitro and molecular docking method. Aqueous extract of A. ilicifolius leaves was made, and its therapeutic potential was examined using half cytotoxic concentration (CC50) and effective concentration (EC50) on Vero cells using the MTT method. The selectivity index (SI) value was determined from the ratio of CC50 and EC50. A total of 12 ligand compounds reported to be contained in the extract were prepared for molecular docking using AutoDock Vina, followed by in silico drug-like potential.  A. ilicifolius water extract achieved the greatest Vero cell viability rate of 159.80% at a concentration of 31.25 μg/mL. A. ilicifolius extract at 100 μg/mL demonstrated the greatest DENV-3 inhibitory rate of 79.69%. The CC50 value was 78.55 μg/ml, and the EC50 value was 64.37 μg/mL.  The SI value calculated from the ratio of CC50 to EC50 was 1.22. Molecular docking identified stigmasterol, stigmasterol-3-O-β-d-glucopyranoside, and quercetin as the primary compounds with the greatest docking values (-7.9 kcal/mol, -7.6 kcal/mol, and -7.7 kcal/mol, respectively).  The findings of this study demonstrate the anti-DENV capability of the A. ilicifolius extract compound, which can limit viral multiplication via biological agents that interact with virus attachment within host cells, with an SI value of 1.22.
PEMANFAATAN BIO-BRIKET BERBAHAN DASAR LIMBAH AMPAS TEBU DAN BAGLOG JAMUR TIRAM SEBAGAI ALTERNATIF ENERGI RAMAH LINGKUNGAN Elizabeth, Gladys; Aprodita, Ni Wayan Seila; Aryaswari, Ni Kadek Dwi Liopita; Wiradana, Putu Angga
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 8 (2025): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v8i.5243

Abstract

ABSTRAK Arang dari limbah produksi pertanian atau turunannya umumnya tidak bernilai ekonomis sehingga seringkali menyebabkan pencemaran lingkungan. Limbah ampas tebu dan baglog jamur tiram merupakan salah dua dari berbagai macam produk turunan dari kegiatan pertanian. Selama ini, ampas tebu lebih tinggi dihasilkan jika dibandingkan dengan gula tebunya pada tahap produksi. Penelitian ini bertujuan untuk memperkenalkan Inovasi Bio-Briket sebagai salah satu sumber energi alternatif yang dapat digunakan sebagai solusi untuk mengurangi permasalahan meningkatnya limbah organik yang menjadi ancaman serius bagi lingkungan. Limbah yang digunakan sebagai bahan baku pembuatan Bio-Briket sangat mudah ditemukan dan merupakan by-product dari hasil kegiatan pertanian seperti ampas tebu dan limbah baglog. Pemanfaatan bahan baku yang berasal dari biomassa menjadi Bio-Briket juga bersifat ramah lingkungan karena selama ini bahan baku tersebut cenderung belum dimanfaatkan secara optimal oleh petani atau masyarakat dan belum memiliki nilai ekonomis yang tinggi. Pada saat digunakan, Bio-Briket ini juga lebih ramah lingkungan karena menghasilkan asap pembakaran yang relatif lebih rendah dibandingkan arang biasa, sehingga diharapkan mampu mengurangi dampak polusi udara dan menunjang kesehatan masyarakat. Bio-Briket yang ramah lingkungan dan bernilai ekonomis. Inovasi baru dari Bio-Briket yang menggunakan ampas tebu dan limbah baglog jamur tiram ini juga dapat mendukung 17 Program Sustainable Development Goals (SDGs) terutama pada poin 7 (Affordable and Clean Energy), poin 12 (Responsible Consumption and Production), dan poin 13 (Climate Action).
Antidyslipidaemic effect of Ipomoea batatas L. extract with increased PCSK-9 levels in atherosclerotic rats Yasa, I Wayan Putu Sutirta; Jawi, I Made; Mahendra, Agung Nova; Wiradana, Putu Angga; I Gede Widhiantara
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.7260

Abstract

Atherosclerosis is the largest cause of mortality worldwide, and Low-Density Lipoprotein (LDL) is a primary risk factor for this illness. The purpose of this research was to determine the effect of purple sweet potato (Ipomoea batatas L.) extract on cholesterol, LDL, PCSK9, and MDA levels in rats fed a high-cholesterol diet. This true experimental study used a randomised pre-test-post-test control group design. Wistar rats were randomly assigned to three groups. Purple sweet potato extract (PSPE) was prepared by 24-hour maceration with 70% ethanol. All rats were fed a high-cholesterol diet for 14 days, followed by pre-test measurements of cholesterol, LDL, PCSK9, and MDA on day 15. Rats were then randomly divided into control (no treatment), PSPE (200 mg/day), and Simvastatin (0.9 mg/kg BW) groups. After one month of treatment, post-test analyses were performed for all parameters. The results showed an increase in serum cholesterol, LDL and MDA levels in rats fed a high-cholesterol diet (p<0.05) compared to the PSPE and Simvastatin groups. The high-cholesterol diet also resulted in a significant (p<0.05) decrease in PCSK9 when compared to the PSPE and Simvastatin groups. In the PSPE group there was a decrease in cholesterol, LDL, and MDA, and PSPE (p<0.05) compared to the control group. In the Simvastatin group, the increase in PCSK9 was very low compared to the PSPE group. PSPE has antidyslipidaemic activity by reducing total cholesterol status, LDL, and oxidative stress while promoting PCSK-9 function. Further research is still needed to provide a comprehensive understanding of the mechanism of action of PSPE as an antioxidant source in patients with atherosclerosis.
Co-Authors Abd. Rahman As-syakur Adam Saba Anggara Adi Ariyanto Wibisono Adi Sofyan Ansori, Muhammad Adnorita Fandah Oktariani Afta Daniel Zato Waruwu Agoes Soegianto Agung Nova Mahendra Agung Wahyu Permadi Agustono Agustono Anak Agung Ayu Putri Permatasari Anak Agung Ngurah Subawa Aondohemba Samuel Nege Aprodita, Ni Wayan Seila Aris Jatmiko Aryaswari, Ni Kadek Dwi Liopita Aulia Iefan Datya Bagus Komang Satriyasa Baladrat, Nur Komariah Bambang Pontjo Priosoeryanto Bambang Yulianto Bambang Yulianto Corne, Yohanes Deny Suhermawan Yusup Dewa Ayu Angga Pebriani Dewa Ayu Intan Tirta Sari Dewi, Pande Putu Indira Prima Eka Putri Suryantari Elizabeth, Gladys Eva Harlina Fahror Rosi Fimaputra, Jatu Maranatha Fransiskus Jimmy Roga Garry Ganvis Jonteo Hani Gede Surya Indrawan Hanipa, Petronela Herdiansyah, Mochammad Aqilah I Gede Widhiantara I Ketut Wija Negara I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Saka Wijaya I Made Sara Wijana I Made Sara Wijana I Made Wisnu Adhi Putra I Wayan Putu Sutirta Yasa I Wayan Rosiana Ida Ayu Mirah Meliana Dewi Juniartini, Nyoman Sweet Kismono Kudoasmoro Komang Kartika Indi Swari Kusala, Muhammad Khaliim Jati Kusala Kusuma, Nathania Eka Putri Lanniari, Nafrina Luh Putu Widiastini M. Rheza Rizki Syahputra Made Nyandra MADE PHARMAWATI Matilda, Yuliana Matius Victorino Ola Dame Mirni Lamid Moch. Nurhudah Mochammad Aqilah Herdiansyah Muhammad Taufiq Shidqi Naw, Sin War Ni Kadek Dwipayani Lestari NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Luh Sunarsih Ni Luh Watiniasih Ni Made Wagi Ambakesari Ni Putu Adriani Astiti Ni Putu Putri Wijayanti Ni Wayan Ayu Wiartini Ni Wayan Deswiniyanti Novaria Sari Dewi Panjaitan Nurhudah, Moch. Nurina, Dien Uma Nwet Darli Kyaw Zaw Okabayashi, Tamaki Panjaitan, Novaria Sari Dewi Panjaitan, Novaria Sari Dewi Panjaitan Pelupessy, Yesha Ainesis El Gracianita Permatasari , Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Prastyadi Wibawa Rahayu Putri, Fadillaisyia Riandani Putu Eka Sudaryatma Putu Eka Sudaryatma Rachmi Ridho Raden Joko Kuncoroningrat Susilo Rahardja, Viryanando Evan Rahardjo, Sinung Rahmadi Prasetijo Razaq, Imanuddin Retno Wulansari Rizhar Eman Karunia Akbar Rizqy, Aimatun Nisfia Rostantinata, Riski Sari, Ni Kadek Yunita Sari Sektiana, Sinar Pagi Setyo Budi Kurniawan Sin War Naw Sinung Rahardjo Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sugama, I Ketut Surachmi Setiyaningsih Teguh Hari Sucipto, Teguh Hari Utari, Heny Budi Viryanando Evan Rahardja Waruwu, Ernawati Wiartini, Ni Wayan Ayu Wiartini Wibisono, Adi Ariyanto Widiiastuti, Ni Komang Wijaya, I Made Saka Win Darmanto Yesha Ainensis El G. Pelupessy Yohanes Corne