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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Sain Veteriner METAMORFOSA Journal of Biological Sciences Bumi Lestari Biogenesis: Jurnal Ilmiah Biologi Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) The Indonesian Biomedical Journal Jurnal Biologi Tropis Jurnal Perikanan Universitas Gadjah Mada Jurnal Medik Veteriner WIDYA LAKSANA Aquacultura Indonesiana Journal of Aquaculture and Fish Health INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Abdi Insani Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology Jurnal Ilmiah Sains Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Seminar Nasional Aplikasi Iptek (SINAPTEK) Jurnal Kesehatan Terpadu JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) DEDIKASI : Jurnal Pengabdian Kepada Masyarakat Paradharma: Jurnal Aplikasi IPTEK Journal of Fisheries & Marine Biosaintifika: Journal of Biology & Biology Education Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Putu Angga Wiradana
Program Studi Biologi, Fakultas Ilmu Kesehatan, Sains, Dan Teknologi, Universitas Dhyana Pura
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
The effectiveness of maggot and probiotic feed supplementation on the growth performance and intestinal microbiota of Indonesian shortfin eel, Anguilla bicolor bicolor Sutrisno, Sutrisno; Otie Dylan Soebhakti Hasan; Triyanto , Triyanto; Putu Angga Wiradana; Putu Eka Sudaryatma
Jurnal Akuakultur Indonesia Vol. 25 No. 1 (2026): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.25.1.105-118

Abstract

The purpose of this study was to determine the effectiveness of probiotic and maggot flour supplementation on production performance, water quality, and eel gut microbiota. There were five treatment groups of maggot flour doses (0, 25, 50, 75, and 100%) each with three replications. The eel seeds used were three months old (average weight 6.11 ± 4.28 g, average length 14.3 ± 3.22 cm) as many as 15 fish/aquarium which were fed until full twice a day for 60 days. Growth performance measurements were carried out every two weeks and at the end of the study, survival rate, feed digestibility, water quality, and the abundance of water and eel gut bacteria. The growth rate of group P1 (0% maggot flour) of 1.222 ± 0.662 g/day was significantly higher (P≤0.05) than group P5 (100% maggot flour) of 0.223 ± 0.094 g/day. The best FCR value was shown by treatment P1 of 2.576 ± 0.598. The highest protein digestibility was shown by group P5 at 75.90%, while the lowest protein digestibility was shown by group P1 at 62.41%. Low digestible protein and high fecal protein were shown by group P5 of 15.15% and 28.77%, respectively. The abundance of bacteria in the Bacillus sp. and Lactobacillus sp. groups dominated in groups P2 and P5. Based on the research results, it can be concluded that the use of commercial probiotics and 100% dose of maggot flour is not yet effective in improving growth performance, but can increase the abundance of good bacteria in the intestines of eels. Keywords: Anguilla bicolor, growth parameters, gut microbiota, maggot flour, probiotic   Abstrak Tujuan penelitian ini adalah untuk mengetahui efektivitas suplementasi probiotik dan tepung maggot terhadap performa produksi, kualitas air, dan mikrobiota usus sidat. Terdapat lima kelompok perlakuan dosis tepung maggot (0, 25, 50, 75, dan 100%) masing-masing dengan tiga kali ulangan. Benih sidat yang digunakan berumur tiga bulan (berat rata-rata 6.11 ± 4.28 g, panjang rata-rata 14.3 ± 3.22 cm) sebanyak 15 ekor/akuarium yang diberi pakan sampai kenyang sebanyak dua kali sehari selama 60 hari. Pengukuran performa pertumbuhan dilakukan setiap dua minggu sekali dan akhir penelitian, tingkat kelangsungan hidup, daya cerna pakan, kualitas air, serta kelimpahan air dan bakteri usus belut. Laju pertumbuhan kelompok P1 (0% tepung maggot) sebesar 1,222 ± 0,662 g/hari lebih tinggi secara signifikan (P≤0,05) dibandingkan kelompok P5 (100% tepung maggot) sebesar 0,223 ± 0,094 g/hari. Nilai FCR terbaik ditunjukkan oleh perlakuan P1 sebesar 2,576 ± 0,598. Daya cerna protein tertinggi ditunjukkan oleh kelompok P5 sebesar 75,90%, sedangkan daya cerna protein terendah ditunjukkan oleh kelompok P1 sebesar 62,41%. Protein cerna rendah dan protein feses tinggi ditunjukkan oleh kelompok P5 masing-masing sebesar 15,15% dan 28,77%. Kelimpahan bakteri pada kelompok Bacillus sp. dan Lactobacillus sp. mendominasi pada kelompok P2 dan P5. Berdasarkan hasil penelitian dapat disimpulkan bahwa penggunaan probiotik komersial dan tepung maggot dosis 100% belum efektif dalam meningkatkan kinerja pertumbuhan, tetapi mampu meningkatkan kelimpahan bakteri baik dalam usus dari ikan sidat. Kata kunci: Anguilla bicolor, mikrobiota usus, parameter pertumbuhan, probiotik, tepung maggot
Evaluation of Mangrove, Acanthus ilicifolius Aqueous Extract on Inhibition of Dengue Virus Replication: In Vitro and Molecular Docking Approach Wiradana, Putu Angga; Wiartini, Ni Wayan Ayu Wiartini; Sucipto, Teguh Hari; Herdiansyah, Mochammad Aqilah; Permatasari, Anak Agung Ayu Putri; Widhiantara, I Gede; Sari, Ni Kadek Yunita Sari; Darmanto, Win; Panjaitan, Novaria Sari Dewi Panjaitan; Kusala, Muhammad Khaliim Jati Kusala
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.71217

Abstract

Dengue virus (DENV) infection is a worldwide health risk, presenting severe symptoms with possibly lethal consequences. The search for effective antiviral drugs against DENV has largely focused on natural products. Mangrove, Acanthus ilicifolius, has been empirically shown to exhibit various biological activities and good safety profiles.  The purpose of this investigation was to examine the antiviral activity of A. ilicifolius aqueous extract against DENV, and the way it functions of action of its bioactive components function was explored utilizing an in vitro and molecular docking method. Aqueous extract of A. ilicifolius leaves was made, and its therapeutic potential was examined using half cytotoxic concentration (CC50) and effective concentration (EC50) on Vero cells using the MTT method. The selectivity index (SI) value was determined from the ratio of CC50 and EC50. A total of 12 ligand compounds reported to be contained in the extract were prepared for molecular docking using AutoDock Vina, followed by in silico drug-like potential.  A. ilicifolius water extract achieved the greatest Vero cell viability rate of 159.80% at a concentration of 31.25 μg/mL. A. ilicifolius extract at 100 μg/mL demonstrated the greatest DENV-3 inhibitory rate of 79.69%. The CC50 value was 78.55 μg/ml, and the EC50 value was 64.37 μg/mL.  The SI value calculated from the ratio of CC50 to EC50 was 1.22. Molecular docking identified stigmasterol, stigmasterol-3-O-β-d-glucopyranoside, and quercetin as the primary compounds with the greatest docking values (-7.9 kcal/mol, -7.6 kcal/mol, and -7.7 kcal/mol, respectively).  The findings of this study demonstrate the anti-DENV capability of the A. ilicifolius extract compound, which can limit viral multiplication via biological agents that interact with virus attachment within host cells, with an SI value of 1.22.
PEMANFAATAN BIO-BRIKET BERBAHAN DASAR LIMBAH AMPAS TEBU DAN BAGLOG JAMUR TIRAM SEBAGAI ALTERNATIF ENERGI RAMAH LINGKUNGAN Elizabeth, Gladys; Aprodita, Ni Wayan Seila; Aryaswari, Ni Kadek Dwi Liopita; Wiradana, Putu Angga
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 8 (2025): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v8i.5243

Abstract

ABSTRAK Arang dari limbah produksi pertanian atau turunannya umumnya tidak bernilai ekonomis sehingga seringkali menyebabkan pencemaran lingkungan. Limbah ampas tebu dan baglog jamur tiram merupakan salah dua dari berbagai macam produk turunan dari kegiatan pertanian. Selama ini, ampas tebu lebih tinggi dihasilkan jika dibandingkan dengan gula tebunya pada tahap produksi. Penelitian ini bertujuan untuk memperkenalkan Inovasi Bio-Briket sebagai salah satu sumber energi alternatif yang dapat digunakan sebagai solusi untuk mengurangi permasalahan meningkatnya limbah organik yang menjadi ancaman serius bagi lingkungan. Limbah yang digunakan sebagai bahan baku pembuatan Bio-Briket sangat mudah ditemukan dan merupakan by-product dari hasil kegiatan pertanian seperti ampas tebu dan limbah baglog. Pemanfaatan bahan baku yang berasal dari biomassa menjadi Bio-Briket juga bersifat ramah lingkungan karena selama ini bahan baku tersebut cenderung belum dimanfaatkan secara optimal oleh petani atau masyarakat dan belum memiliki nilai ekonomis yang tinggi. Pada saat digunakan, Bio-Briket ini juga lebih ramah lingkungan karena menghasilkan asap pembakaran yang relatif lebih rendah dibandingkan arang biasa, sehingga diharapkan mampu mengurangi dampak polusi udara dan menunjang kesehatan masyarakat. Bio-Briket yang ramah lingkungan dan bernilai ekonomis. Inovasi baru dari Bio-Briket yang menggunakan ampas tebu dan limbah baglog jamur tiram ini juga dapat mendukung 17 Program Sustainable Development Goals (SDGs) terutama pada poin 7 (Affordable and Clean Energy), poin 12 (Responsible Consumption and Production), dan poin 13 (Climate Action).
Co-Authors Abd. Rahman As-syakur Adam Saba Anggara Adi Ariyanto Wibisono Adi Sofyan Ansori, Muhammad Adnorita Fandah Oktariani Afta Daniel Zato Waruwu Agoes Soegianto Agung Wahyu Permadi Agustono Agustono Anak Agung Ayu Putri Permatasari Anak Agung Ngurah Subawa Aondohemba Samuel Nege Aprodita, Ni Wayan Seila Aris Jatmiko Aryaswari, Ni Kadek Dwi Liopita Aulia Iefan Datya Bagus Komang Satriyasa Baladrat, Nur Komariah Bambang Pontjo Priosoeryanto Bambang Yulianto Bambang Yulianto Corne, Yohanes Deny Suhermawan Yusup Dewa Ayu Angga Pebriani Dewa Ayu Intan Tirta Sari Dewi, Pande Putu Indira Prima Eka Putri Suryantari Elizabeth, Gladys Eva Harlina Fahror Rosi Fimaputra, Jatu Maranatha Fransiskus Jimmy Roga Garry Ganvis Jonteo Hani Gede Surya Indrawan Hanipa, Petronela Herdiansyah, Mochammad Aqilah I Gede Widhiantara I Ketut Wija Negara I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Saka Wijaya I Made Sara Wijana I Made Sara Wijana I Made Wisnu Adhi Putra I Wayan Putu Sutirta Yasa I Wayan Rosiana Ida Ayu Mirah Meliana Dewi Juniartini, Nyoman Sweet Kismono Kudoasmoro Komang Kartika Indi Swari Kusala, Muhammad Khaliim Jati Kusala Kusuma, Nathania Eka Putri Lanniari, Nafrina Luh Putu Widiastini M. Rheza Rizki Syahputra Made Nyandra MADE PHARMAWATI Matilda, Yuliana Matius Victorino Ola Dame Mirni Lamid Moch. Nurhudah Mochammad Aqilah Herdiansyah Muhammad Taufiq Shidqi Naw, Sin War Ni Kadek Dwipayani Lestari NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Luh Sunarsih Ni Luh Watiniasih Ni Made Wagi Ambakesari Ni Putu Adriani Astiti Ni Putu Putri Wijayanti Ni Wayan Ayu Wiartini Ni Wayan Deswiniyanti Novaria Sari Dewi Panjaitan Nurhudah, Moch. Nurina, Dien Uma Nwet Darli Kyaw Zaw Okabayashi, Tamaki Otie Dylan Soebhakti Hasan Panjaitan, Novaria Sari Dewi Panjaitan, Novaria Sari Dewi Panjaitan Pelupessy, Yesha Ainesis El Gracianita Permatasari , Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Prastyadi Wibawa Rahayu Putri, Fadillaisyia Riandani Putu Eka Sudaryatma Putu Eka Sudaryatma Rachmi Ridho Raden Joko Kuncoroningrat Susilo Rahardja, Viryanando Evan Rahardjo, Sinung Rahmadi Prasetijo Razaq, Imanuddin Retno Wulansari Rizhar Eman Karunia Akbar Rizqy, Aimatun Nisfia Rostantinata, Riski Sari, Ni Kadek Yunita Sari Sektiana, Sinar Pagi Setyo Budi Kurniawan Sin War Naw Sinung Rahardjo Sri Puji Astuti Wahyuningsih Sugama, I Ketut Surachmi Setiyaningsih Sutrisno, Sutrisno Teguh Hari Sucipto, Teguh Hari Triyanto , Triyanto Utari, Heny Budi Viryanando Evan Rahardja Waruwu, Ernawati Wiartini, Ni Wayan Ayu Wiartini Wibisono, Adi Ariyanto Widiiastuti, Ni Komang Wijaya, I Made Saka Win Darmanto Yesha Ainensis El G. Pelupessy Yohanes Corne