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Isolation and Molecular Identification of Endophytic Fungi Associated with Brown Algae for Inhibiting Escherichia coli ESBL Wiradana, Putu Angga; Permatasari , Anak Agung Ayu Putri; Sari, Ni Kadek Yunita; Widhiantara, I Gede; Sandhika, I Made Gde Sudyadnyana; Waruwu, Ernawati; Fimaputra, Jatu Maranatha; Panjaitan, Novaria Sari Dewi; Putri, Fadillaisyia Riandani; Ansori, Arif Nur Muhammad

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Abstract

Background: The marine environment is the main source of research on natural products in the future. In addition, marine microorganisms have been identified as a natural source capable of developing new antibiotic compounds, including controlling urinary tract infections caused by Escherichia coli ESBL. Purpose: This study aims to isolate, select, and test the potential of brown macroalgae endophytic fungi (Phaeophyceae) collected from the coastal waters of Sanur, Bali Province. Methods: Three types of brown algae were collected from Sanur Beach and their endophytic fungi were isolated using PDA media. Antibacterial activ
Effect of vitamin C supplementation on the survival rate and histopathological changes of gills and kidneys of tilapia (Oreochromis niloticus) infected by Aeromonas hydrophylla Dewa Ayu Angga Pebriani; I Ketut Wija Negara; Ni Putu Putri Wijayanti; Sudaryatma, Putu Eka; Putu Angga Wiradana
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.43897

Abstract

This study was conducted to determine the effect of vitamin C supplementation on the survival rate and histopathological changes of gills and kidneys of tilapia infected by Aeromonas hydrophila. Three doses of vitamin C were tested (150; 300; and 450 mg/kg) with two control groups. Tilapia with uniform size (average weight of 14 grams) as the criteria for inclusion were randomly distributed in five ponds with 15 tilapia fish per ponds. Tilapia was fed with hands until full for two weeks. Tilapia was then infected with A. hydrophila to find out the survival rate and histopathological changes of gills and kidneys at the end of the experimental period or 7 days after infection. The supplementation of 150 mg/kg vitamin C in feed increased the survival rate (%) in Tilapia by 86.67% or higher than other treatment and control groups. The damage to gills in terms of lamella separation was found in all treatment groups, as well as kidneys. The results found that the supplementation of 150 mg/kg vitamin C in feed increased survival rate but did not give effective protection on gills and kidneys.
Effect of Silk Worms (Tubifex sp.) Feed On Production Performance, Nutri-tion, And Gills Histology of Striped Catfish Fingerlings (Pangasionodon hy-popthalmus): English Yohanes Corne; Sinung Rahardjo; Moch. Nurhudah; Putu Angga Wiradana; Sudaryatma, Putu Eka
Journal of Aquaculture and Fish Health Vol. 14 No. 1 (2025): JAFH Vol. 14 No. 1 February 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i1.62255

Abstract

Natural feed has emerged as an alternative to support production performance and the nutrients contained in fish meat. This study aimed to determine the effect of feeding silk worms (Tubifex sp.) in improving production performance, nutritional content, and gill histology of striped catfish fingerlings (Pangasionodon hypopthalmus). Silkworms were sourced from different origins, namely Group P1 (silkworms with dry food media), Group P2 (commercial silkworms), and Group P3 (wild silkworms). Silkworms were reared for 55 days and fed to catfish seed for 25 days ad libitum.  Feeding had no significant effect on absolute length growth, total biomass, and feed conversion with worm feeding treatment (P1) with values respectively 2.3 ± 0.10%, 6.0 ± 0.38%, and 4.2 ± 0.47%. Likewise, the results of growth rate, weight, and length, group P1 had better results compared to treatments (P2) and (P3) but were not significantly different. The best protein efficiency ratio resulted from treatment (P1) of 0.42 ± 0.25%. Gill histology changes were shown in groups P2 and P3, namely hemorrhage and congestion. Based on these results, silkworms reared in dry media can be an effective natural food for striped catfish fingerlings.
Evaluasi Potensi Antibakteri Ekstrak Etanol Jahe Merah (Zingiber officinale var. rubrum Theilade) terhadap Bakteri Methicillin Resistant Staphylococcus aureus secara In Vitro Widiiastuti, Ni Komang; Sari, Ni Kadek Yunita; Wiradana, Putu Angga
JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) Vol. 3 No. 1 (2024): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/js.v3i1.2936

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Methicillin-resistant Staphylococcus aureus (MRSA) merupakan strain bakteri patogen Staphylococcus aureus yang resisten terhadap antibiotik beta lactam khususnya metisilin. Infeksi MRSA hanya dapat diterapi dengan antibiotik tertentu dan sangat terbatas. Hingga saat ini, kasus resistensi terus berkembang sebagai dampak dari penggunaan antibiotik yang tidak bijaksana. Tujuan penelitian ini yaitu untuk mengetahui secara ilmiah melalui uji praklinik evaluasi antibakteri ekstrak jahe merah sebagai obat herbal terstandar (OHT) di masa mendatang. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan 5 kali ulangan dan 3 perlakuan. Uji konfirmatif isolat bakteri MRSA dilakukan dengan parameter uji pewarnaan gram, uji katalase, kultur pada media MSA, dan uji sensitifitas terhadap antibiotik. Uji daya hambat antibakteri dilakukan dengan metode difusi sumuran. KHM ditentukan dengan mengukur nilai absorbansi pada Spektrofotometer UV-Vis. KBM ditentukan dengan melihat tidak adanya pertumbuhan bakteri pada cawan Petri. Hasil uji konfirmatif menunjukkan bahwa isolat bakteri merupakan bakteri MRSA. Rerata daya hambat tertinggi pada ekstrak jahe merah yaitu sebesar 7,6 mm pada konsentrasi 50%. KHM ekstrak jahe merah terdapat pada konsentrasi 25%. KBM ekstrak jahe merah terdapat pada konsentrasi 100%. Ekstrak jahe merah mampu menghambat pertumbuhan bakteri MRSA. Semakin rendah konsentrasi, daya hambat semakin besar.
DIET TINGGI KOLESTEROL MENURUNKAN SEKRESI HORMON TESTOSTERON: STUDI LITERATUR I Gede Widhiantara; Sandhika, I Made Gde Sudyadnyana; I Wayan Rosiana; Putu Angga Wiradana
Jurnal Kesehatan Terpadu Vol. 7 No. 1 (2023): Jurnal Kesehatan Terpadu
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/jkt.v7i1.2496

Abstract

Kajian ini bertujuan untuk mengetahui bahwa pola makan tinggi kolesterol menyebabkan kadar kolesterol dalam darah dan jaringan melebihi kadar normal sehingga meningkatkan produksi radikal bebas dalam tubuh terutama lipid peroksida yang merupakan salah satu penyebab infertilitas pada pria. Telah banyak penelitian yang membuktikan hubungan peningkatan radikal bebas dengan gangguan fungsi reproduksi pria khususnya penurunan sekeresi testosteron. Testosteron merupakan hormon reproduksi utama pada pria atau hewan jantan yang dihasilkan melalui proses steroidogenesis pada sel Leydig testis. Dampak tingginya kadar kolesterol dalam darah menurunkan sekresi testosteron antara lain melalui peningkatan produksi radikal bebas sehingga menghambat sekresi Luteinizing Hormone (LH), menghambat enzim-enzim atau protein yang berperan dalam steroidogenesis seperti StAR, enzim sitokrom p450scc, 3β-hydroxysteroid dehydrogenase, 17β- hydroxysteroid dehydrogenase, dan secara seluler merusak morfologi serta fungsi sel Leydig.
Pendampingan Herbal Dan Pelatihan Senam Jantung Untuk Lansia di Desa Celuk, Sukawati, Gianyar Widhiantara, I Gede; Rosiana, I Wayan; Wiradana, Putu Angga; Sandhika, I Made Gde Sudyadnyana; Permadi, Agung Wahyu; Jawi, I Made
Paradharma: Jurnal Aplikasi IPTEK Vol. 6 No. 2 (2022): Paradharma: Jurnal Aplikasi IPTEK
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Dhyana Pura – Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (566.522 KB) | DOI: 10.36002/jpd.v6i2.2395

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ABSTRAKPopulasi penduduk lansia di Desa Celuk, Sukawati, Gianyar, cukup tinggi dengan rata-rata 200 orang di tiap banjar. Sebagian besar lansia menderita keluhan hipertensi yang merupakan faktor resiko penyakit jantung dan stroke. Hasil wawancara dengan beberapa penduduk Desa Celuk bahwa masyarakat lokal secara empiris masih mempertahankan tradisi pemanfaatan herbal untuk mencegah ataupun mengobati penyakit namun kurang memahami cara pengolahan, dosis dan manfaat yang tepat. Program pengadian ini dilaksanakan selama 5 bulan di Desa Adat Celuk, Sukawati Gianyar. Tujuan dari program ini yaitu mengedukasi manfaat tanaman herbal untuk pencegahan dan terapi keluhan kesehatan lansia, melatih mengolah minuman herbal yang higienis, tepat dosis, meningkatkan kesehatan fisik lansia melalui kombinasi minuman herbal dan aktifitas fisik melalui latihan senam jantung. Metode yang diterapkan pada kegiatan ini berupa pendampingan dan pelatihan. Target dan luaran dari program pengabdian ini adalah meningkatkan pengetahuan dan keterampilan lansia dalam mengolah minuman herbal dan meningkatkan kesehatan fisik lansia melalui latihan senam jantung. Hasil kegiatan terjadi peningkatan pemahaman dan keterampilan lansia rata-rata sebesar 50,83 % dalam budidaya dan membuat minuman herbal serta melakukan senam kesehatan jantung.Kata kunci: herbal, latihan fisik, lansia, CelukABSTRACTThe elderly population in Celuk Village, Sukawati, Gianyar, is relatively high, with an average of 200 people in each Banjar. Most of the elderly suffer from hypertension, a risk factor for heart disease and stroke. The results of interviews with several residents of Celuk Village show that the local community empirically still maintains the tradition of using herbs to prevent or treat disease but does not understand how to process them, the proper dosage, and their benefits. This court program was held for 5 months in the Celuk Traditional Village, Sukawati Gianyar. The aim of this program is to educate the benefits of herbal plants for the prevention and treatment of elderly health complaints, train them to process herbal drinks that are hygienic, in the right dosage, improve the physical health of the elderly through a combination of herbal beverages and physical activity through heart exercises. The method applied to this activity is in the form of mentoring and training. The target and outcome of this service program are to increase the knowledge and skills of the elderly in processing herbal drinks and improve their physical health of the elderly through heart exercises. The results of the activity showed an increase in the understanding and skills of the elderly on average by 50.83% in cultivating and making herbal drinks and doing heart health exercises.Keywords: herbs, physical exercise, elderly, Celuk
Edukasi dan Pelatihan Biopori Untuk Sivitas Akademika Universitas Dhyana Pura Menuju Kampus Hijau Berkelanjutan Widhiantara, I Gede; Angga Wiradana, Putu; Prasetijo, Rahmadi
Paradharma: Jurnal Aplikasi IPTEK Vol. 9 No. 1 (2025): Paradharma: Jurnal Aplikasi IPTEK
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Dhyana Pura – Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/jpd.v9i1.4884

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Penerapan gaya hidup yang ramah lingkungan dan berkelanjutan menjadi aspek krusial di lingkungan perguruan tinggi. Sejumlah universitas telah menginisiasi berbagai inovasi dan menetapkan program jangka panjang yang dikenal sebagai green campus. Universitas Dhyana Pura (Undhira) di Bali merupakan salah satu institusi yang mengadopsi kebijakan green campus dengan tujuan mewujudkan lingkungan kampus yang hijau dan berkelanjutan, sesuai dengan indikator yang ditetapkan oleh UI GreenMetric World University Ranking Salah satu parameter penting dalam menciptakan kampus hijau berkelanjutan adalah program atau kegiatan konservasi air di kampus dan belum ada langkah nyata untuk itu. Sehingga ceramah dan pelatihan tentang konservasi air dengan model biopori dilakukan untuk pendekatan konservasi air yang murah dan efektif mengingat ruang terbuka hijau Undhira cukup luas. Hasil pendampingan menunjukkan peningkatan pemahaman dan keterampilan sivitas akademika Undhira dalam membuat biopori.
Occurrence and Consumer Health Risk Assessment of Heavy Metals in Frozen Demersal Fish and Cephalopod Products from Benoa Port, Bali Province Putu Angga Wiradana; I Made Gde Sudyadnyana Sandhika; Putu Eka Sudaryatma; I Gede Widhiantara; Made Nyandra; Adnorita Fandah Oktariani; Setyo Budi Kurniawan
JURNAL KESEHATAN LINGKUNGAN Vol. 16 No. 1 (2024): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v16i1.2024.41-50

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Introduction: Heavy metal pollution has become an important environmental issue today. This study was aimed to identify the heavy metals of the two fishing products namely demersal fish and cephalopods landed at Benoa Port, Bali Province and their health risk effects on consumers. Methods: Demersal fish and cephalopod products were obtained from local fishermen at Benoa port. Atomic Absorption Spectrophotometry (AAS) was used to specify the (Pb, Cd, and Hg) in 34 fish fillet products weighing an average of 500 grams each. Statistical software was used to conduct an analysis of the collected data, while THQ, TTHQ, and TCR were calculated and compared with USEPA regulations. Results and Discussion: The results of this research showed that the content of heavy metals in demersal fish and cephalopod products was lower than the levels suggested by official agencies in a number of countries. In populations of various ages, the target Hazard Quotient (THQ) for the heavy metal was less than 1. At this point, the Total THQ result did not imply any risk to human health from ingestion of these two fishery products. When these two products were ingested, the target cancer risk (TCR) did not represent a cancer risk. Conclusion: The outcomes of this study showed that the bioaccumulation levels of both fishery products with low levels of heavy metals are safe for human consumption.
Prevalence of bacterial contamination on seafoods products collected from traditional fish market in Bali Province during 2023: Prevalensi kontaminasi bakteri pada produk hasil ikan laut yang dikumpulkan dari pasar ikan tradisional di Provinsi Bali selama tahun 2023 Sudaryatma, Putu Eka; Wiradana, Putu Angga; Razaq, Imanuddin; Sunarsih, Ni Luh; Jatmiko, Aris; Permatasari, Anak Agung Ayu Putri; Sari, Ni Kadek Yunita; Widhiantara, I Gede; Sandhika, I Made Gde Sudyadnyana; Rosiana, I Wayan
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 3 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i3.61627

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Seafood provides essential nutrients beneficial for human health; however, it is highly vulnerable to harmful bacterial infections that pose significant public health risks. This research seeks to assess the prevalence of five categories of seafood obtained from various traditional fish markets in Bali Province. A total of 108 tuna samples, 78 pelagic fish samples, 37 cephalopod samples, 14 sardine samples, and 53 demersal fish samples were collected from various traditional markets in Bali Province. This research evaluated the prevalence of E. coli, coliforms, Salmonella, V. cholerae, and V. parahaemolyticus. The study revealed that the highest prevalence of E. coli, coliform, and V. parahaemolyticus contamination in tuna samples was 95 (87%), 95 (87%), and 103 (95%), respectively. The study indicated that E. coli and coliforms were present in 73 of the 78 pelagic fish samples, representing 93% contamination. Sardine samples exhibited the lowest prevalence of bacteria. All seafood samples, however, tested negative for Salmonella and V. cholera. PCR products from E. coli and V. parahaemolyticus isolates were effectively amplified for the target genes utilized in this study. Local seafood markets should adopt appropriate handling and storage practices to enhance seafood quality. This study emphasizes the significant presence of E. coli, coliforms, and V. parahaemolyticus in seafood, along with the potential health risks posed by specific strains and their antibiotic resistance.
SYNTHESIS AND CHARACTERIZATION OF CHITOSAN MEMBRANES OF CASSAVA PEEL (Manihot esculenta) AND LERI STARCH COMBINATION AS A BIODEGRADABLE PLASTIC: English Dewi, Pande Putu Indira Prima; Widhiantara, I Gede; Sandhika, I Made Gde Sudyadnyana; Wiradana, Putu Angga; Susilo, Raden Joko Kuncoroningrat
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3080

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Problems related to landfills of plastic packaging waste are a source of environmental pollution. This research provides innovation in utilizing biodegradable plastic from cassava and leri peel starch with the addition of chitosan. This study aimed to analyze the results of the synthesis and characterization of chitosan membranes with a combination of cassava and leri peel starch and determine the biodegradation time of biodegradable plastic in EM-4 bioactivation. This study characterized biodegradable plastic membranes using test parameters, namely the SEM, FTIR, Swelling, and tensile strength tests. The biodegradation test was carried out by immersing biodegradable plastic membranes in an EM-4 bioactator for 7 days. Based on the results obtained from the characterization of biodegradable plastic membranes, namely the swelling test with concentrations of 5:3, 7:3, and 9:3, with yield values ​​of 62.9921%, 69.8902%, and 71.4286%. The SEM test obtained the best results at a concentration of 7:3. FTIR test on biodegradable plastic membranes containing hydroxyl (O-H) and carbonyl (C-O) groups. The tensile test obtained optimum results at the concentration of biodegradable plastic membranes, namely 9:3. As for the biodegradation test that has been carried out, the start time for degradation is 3 days.
Co-Authors Abd. Rahman As-syakur Adam Saba Anggara Adi Ariyanto Wibisono Adi Sofyan Ansori, Muhammad Adnorita Fandah Oktariani Afta Daniel Zato Waruwu Agoes Soegianto Agung Wahyu Permadi Agustono Agustono Anak Agung Ayu Putri Permatasari Anak Agung Ngurah Subawa Aondohemba Samuel Nege Aris Jatmiko Aulia Iefan Datya Bagus Komang Satriyasa Baladrat, Nur Komariah Bambang Pontjo Priosoeryanto Bambang Yulianto Bambang Yulianto Corne, Yohanes Deny Suhermawan Yusup Dewa Ayu Angga Pebriani Dewa Ayu Intan Tirta Sari Dewi, Pande Putu Indira Prima Eka Putri Suryantari Eva Harlina Fahror Rosi Fimaputra, Jatu Maranatha Fransiskus Jimmy Roga Garry Ganvis Jonteo Hani Gede Surya Indrawan Hanipa, Petronela I Gede Widhiantara I Ketut Wija Negara I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Saka Wijaya I Made Sara Wijana I Made Sara Wijana I Made Wisnu Adhi Putra I Wayan Putu Sutirta Yasa I Wayan Rosiana Ida Ayu Mirah Meliana Dewi Juniartini, Nyoman Sweet Kismono Kudoasmoro Komang Kartika Indi Swari Kusuma, Nathania Eka Putri Lanniari, Nafrina Luh Putu Widiastini M. Rheza Rizki Syahputra Made Nyandra MADE PHARMAWATI Matilda, Yuliana Matius Victorino Ola Dame Mirni Lamid Moch. Nurhudah Muhammad Taufiq Shidqi Naw, Sin War Ni Kadek Dwipayani Lestari NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Luh Sunarsih Ni Luh Watiniasih Ni Made Wagi Ambakesari Ni Putu Adriani Astiti Ni Putu Putri Wijayanti Ni Wayan Ayu Wiartini Ni Wayan Deswiniyanti Nurhudah, Moch. Nurina, Dien Uma Nwet Darli Kyaw Zaw Okabayashi, Tamaki Panjaitan, Novaria Sari Dewi Pelupessy, Yesha Ainesis El Gracianita Permatasari , Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Prastyadi Wibawa Rahayu Putri, Fadillaisyia Riandani Putu Eka Sudaryatma Putu Eka Sudaryatma Rachmi Ridho Raden Joko Kuncoroningrat Susilo Rahardja, Viryanando Evan Rahardjo, Sinung Rahmadi Prasetijo Razaq, Imanuddin Retno Wulansari Rizhar Eman Karunia Akbar Rizqy, Aimatun Nisfia Rostantinata, Riski Sektiana, Sinar Pagi Setyo Budi Kurniawan Sin War Naw Sinung Rahardjo Sugama, I Ketut Surachmi Setiyaningsih Teguh Hari Sucipto, Teguh Hari Utari, Heny Budi Viryanando Evan Rahardja Waruwu, Ernawati Wibisono, Adi Ariyanto Widiiastuti, Ni Komang Wijaya, I Made Saka Yesha Ainensis El G. Pelupessy Yohanes Corne