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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Sain Veteriner Bumi Lestari Biogenesis: Jurnal Ilmiah Biologi Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) The Indonesian Biomedical Journal Jurnal Biologi Tropis Jurnal Perikanan Universitas Gadjah Mada Jurnal Medik Veteriner WIDYA LAKSANA Aquacultura Indonesiana Journal of Aquaculture and Fish Health Jurnal Abdi Insani Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology Jurnal Ilmiah Sains Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Seminar Nasional Aplikasi Iptek (SINAPTEK) Jurnal Kesehatan Terpadu JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) DEDIKASI : Jurnal Pengabdian Kepada Masyarakat Paradharma: Jurnal Aplikasi IPTEK Journal of Fisheries & Marine Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Putu Angga Wiradana
Program Studi Biologi, Fakultas Ilmu Kesehatan, Sains, Dan Teknologi, Universitas Dhyana Pura
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Teknik Deteksi Cemaran Avian Influenza Virus (AIV) Pada Daging Unggas Yang Diperdagangkan Di Pasar Tradisional Di Indonesia: Mini Review Ni Made Wagi Ambakesari; Putu Angga Wiradana
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 6 (2023): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v6i.2767

Abstract

Penyakit flu burung (Avian influenza/AI) merupakan penyakit yang disebabkan oleh virus Avian influenza dari famili Orthomyxoviridae, yang bersifat zoonosis dan mempunyai material genetik berupa RNA berpolaritas negatif, dengan diameter 120 nm dan strukturnya berfilamen. Secara klinis, unggas yang telah terinfeksi virus AI menunjukan gejala gangguan sistem neurologis seperti tortikolis, tremor, kesulitan berdiri, kehilangan keseimbangan, dan hingga kematian unggas. Polymerase Chain Reaction (PCR) merupakan salah satu metode enzimatis untuk melipatgandakan (amplification) secara eksponensial suatu sekuen nukleotida tertentu secara in vitro. Teknik PCR sangat umum digunakan dalam deteksi keberaaan virus AI secara dini dan waktu yang relatif singkat. Namun, masih terdapat teknik lainnya yang dikembangkan untuk mempermudah pelacakan virus ini terutama pada lingkungan pasar tradisional yang masih terbatas dilaporkan. Penelitian ini bertujuan untuk mengetahui metode deteksi virus flu burung beserta subtipenya pada daging unggas yang dijual di pasar tradisional di Indonesia. Telaah singkat ini akan mengungkapkan penelitian-penelitian terkait mengenai sebaran virus flu burung dan metode deteksi yang digunakan terutama pada daging unggas yang telah dijual di pasar tradisional. Pengumpulan data diakses pada Google Scholar, PubMed, Scopus, Web of Science (WoS), dan Lembaga pengindeks nasional/internasional lainnya yang diterbitkan dalam kurun waktu 10 tahun terakhir. Telaah singkat ini sangat penting dilakukan untuk memberikan informasi yang komprehensif mengenai teknik yang umum digunakan dalam upaya deteksi penyebaran virus AI, terutama pada daging unggas yang dijual di pasar tradisional serta meningkatkan kewaspadaan melalui deteksi dini terhadap penyebaran virus AI di masa mendatang.
Tinjauan Perbandingan Evektifitas Pemeriksaan Histopatologi Dan FAT (Fluorescent Antibody Test) Pada Virus Rabies: Artikel Review Garry Ganvis Jonteo Hani; Afta Daniel Zato Waruwu; Putu Angga Wiradana; I Gede Widhiantara
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 6 (2023): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v6i.2863

Abstract

Virus rabies merupakan patogen zoonotik yang menyebabkan penyakit serius padamamalia, termasuk manusia. Identifikasi cepat dan akurat dari virus ini penting untukmanajemen kasus dan pencegahan penularan. Tinjauan ini mengevaluasi efektivitas duametode diagnostik utama, yaitu pemeriksaan histopatologi dan Fluorescent Antibody Test(FAT), dalam mendeteksi virus rabies. Pemeriksaan histopatologi, yang melibatkan analisisstruktur sel dan jaringan secara mikroskopis, telah lama menjadi standar dalam diagnosisvirus rabies Namun, kelebihan waktu yang diperlukan untuk memproses sampel dankompleksitas prosedurnya dapat menjadi kendala. FAT menggunakan antibodi fluoresen untukmendeteksi antigen virus rabies secara langsung dalam jaringan yang terinfeksi. Metode inidikenal karena kecepatan hasilnya, tetapi kemungkinan adanya hasil palsu positif dan negatifmemerlukan peninjauan kritis. Peninjauan ini memberikan wawasan mendalam tentangperkembangan terkini dalam diagnosis virus rabies dan memberikan dasar untuk pemilihanmetode diagnostik yang paling efektif dalam konteks penelitian dan pengelolaan penyakit ini.Hasil menunjukan bahwa pemeriksaan FAT lebih efektif karena Metode ini dapat memberikanhasil dalam waktu yang relatif singkat dan Proses FAT lebih sederhana serta peralatan yanglebih mudah diakses dibandingkan Histopatologi.
PKM PENDAMPINGAN STANDARISASI BAHANALAMBAGIGURU DAN SISWA SMK KESEHATAN BALI MEDIKA, DENPASAR I Made Gde Sudyadnyana Sandhika; Ni Kadek Yunita Sari; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Wayan Rosiana; Putu Angga Wiradana; I Made Wisnu Adhi Putra; Ni Kadek Dwipayani Lestari; Rahmadi Prasetijo; I Made Murna
Seminar Nasional Aplikasi Iptek (SINAPTEK) Vol. 6 (2023): PROSIDING SINAPTEK
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pengetahuan dan keterampilan terkait proses standarisasi bahan alamdi SMKKesehatan Bali Medika kurang diperhatikan karena kurikulumnya lebih banyak memuat matapelajaran produktif keperawatan, teknik laboratorium medik dan farmasi. Sebagai salahsatusekolah swasta di Kota Denpasar, sekolah ini masih memiliki alat dan bahan penunjangpraktikum di bidang Sains yang masih sangat terbatas. Konsep dasar standarisasi bahanalam termasuk penting diberikan di Sekolah ini mengingat sekolah ini merupakan sekolahkesehatan dan memiliki jurusan farmasi. Keterampilan standarisasi bahan alamberguna bagisiswa pada saat magang di dunia industri dan pada saat melanjutkan ke jenjang pendidikanyang lebih tinggi. Tujuan dari kegiatan pengabdian masyarakat ini yaitu: 1)Memberikanwawasan mengenai standarisasi meliputi jenis standarisasi, fungsi standarisasi dan metodestandarisasi pada bahan alam khususnya tanaman obat, 2)Memberikan pengetahuanmengenai teknik-teknik standarisasi baik secara spesifik dan non spesifik pada bahan alamkhususnya tanaman obat, dan 3)Mempraktekan secara langsung dalam membuat sediaanekstrak yang berbahan dasar tanaman obat. Hasil dari Pengabdian Masyarakat ini adalah1)Wawasan mitra terkait standarisasi bahan alam meningkat dari rata-rata nilai 55 menjadi86 setelah posttest, 2)Tingkat pengetahuan mitra meningkat dari tidak tahu menjadi tahuakan teknik-teknik standarisasi dan semua anggota mitra yang mengikuti sosialisasimendapatkan rerata nilai pemahaman 84 pada post-test, 3)Penyampaian materi pembuatansediaan ekstrak bahan alam khususnya tanaman obat meningkat dari rata-rata nilai 45 padapretest menjadi 83 setelah posttest.
Penempelan Teritip (Arthropoda) pada Beberapa Jenis Mangrove di Kawasan TAHURA Ngurah Rai Bali Juniartini, Nyoman Sweet; Watiniasih, Ni Luh; Astiti, Ni Putu Adriani; Wiradana, Putu Angga
Bumi Lestari Journal of Environment Vol 24 No 1 (2024)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2024.v24.i01.p01

Abstract

Mangrove ecosystems are able to support biological and ecological life cycles and provide welfare for coastal communities. Pest outbreaks that attack seedlings to mangrove trees often cause ecological losses throughout the TAHURA Ngurah Rai Bali area. The ecological interactions of barnacle pest species in mangrove ecosystems are very complex and highly detailed studies. There is empirical evidence that the attachment of barnacles is strongly associated with allelochemical compounds produced by mangroves. This study aims to inventory the attachment of barnacles (arthropods) to several types of mangroves in the TAHURA Ngurah Rai area of Bali. This study used a purposive sampling method to pay for the location of the observations. Quadrant transects were used to organize the classification of the mangrove plots, barnacle densities being calculated for each transect. The density of barnacles was calculated on the number of barnacles attached to the mangrove plants, then recorded the types of plants attached, types of barnacles, types of planting and the number of individuals for each mangrove classification. The study showed that barnacles planted most of the mangrove seedlings at all stations, while the classification of tree plots only had barnacles attached at station 3. The number of attachments of Amphibalanus sp. the highest was found at station 3 of the R. mucronata tiller classification of 881 individuals, while no attachment of barnacles was found at all stations for S. alba mangroves. Overall, the attachment of barnacles was higher in R. mucronata tillers and had the potential to reduce their growth productivity in the field. Efforts to control barnacles really need to be done to increase the success of planting mangroves in TAHURA Ngurah Rai, Bali.
Status, Trends, and Potentials of Turtle Conservation in Bali: A Mini Review Pelupessy, Yesha Ainesis El Gracianita; Wiradana, Putu Angga; Rosiana, I Wayan; Widhiantara, I Gede
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.256-268

Abstract

Sea turtles in Bali has been exploited excessively, which caused decrease in population, and this issue has placed sea turtles threatened to extinction. This review article aimed to provide information regarding the status, trends, and potential of sea turtles in Bali. Information given in this article is vital to ensure reliable knowledge not to only understand our current situation, but also to increase efficiency in reliant to the problem sea turtles face. The literature study method is used to write this review paper, namely by accessing a number of research paper published nationally and internationally. It was explained that the status of sea turtles is known to be endangered and law enforcement is not sufficient overcoming the problem. Conservation trends such as nurturing hatchlings have shown to not only help restore the population of sea turtles, but also to bring economic benefits to the conservation sites and the people. Potentials of ecotourism and DNA Barcoding has shown to be effective to benefit the people economically and increase the efficiency of law enforcement and conservation. Solutions and methods of improvement such as ecotourism and DNA Barcoding explained in this article is practical for Bali to adapt, so that sea turtle conservation is capable to overcome its status and incline to its potential.
Contaminants and Human Health Risks Associated with Exposure to Microplastic Ingestion of Green Mussels (Perna viridis) Collected from The Kedonganan Fish Market, Bali Wiradana, Putu Angga; Sandhika, I Made Gde Sudyadnyana; Widhiantara, I Gede; Rizqy, Aimatun Nisfia; Soegianto, Agoes; Yulianto, Bambang
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.197-208

Abstract

Microplastics (MPs) are pollutant agents that have been absorbed and detected in aquatic ecosystems at high concentrations. This study aimed to investigate the presence of MPs pollution in green mussel (Perna viridis) products sold at the Kedonganan fish market, Badung, Bali. A total of 150 mussels with an average weight of 3,2 ± 0,71 g/mussels from three traders each composed and followed by the pre-treatment stage using 5 M NaCl solution, extraction with wet oxidation peroxidation (WPO) + Fe(II) catalyst and filtered. The highest percentage for the form of MPs was successively obtained by the Line form in Trader A at 85,42% and the lowest in Trader C at 50,00%. The highest form of fragments was obtained in Trader C at 42,86%. Film and filament forms were only obtained in Trader A. The highest MPs color was black and the lowest was gray. The highest average MPs particle size was found in the form of a filament of 1944,37 ± 88,41 μm which was found in Trader A. Estimates of MPs intake per year/capita in Indonesia showed that exposure to MPs through consumption of green mussels in this study amounted to 498,330 MPs/year/capita items. Overall, the green mussel from Trader A had the highest percentage and size of MPs, with the shape of fragment MPs being dominated by Trader C and the color of the MPs being dominated by black. MPs exposure to green mussel consumption in Indonesia is very high, but no health impact category has yet been found for this estimate.
Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV) Nurhudah, Moch.; Baladrat, Nur Komariah; Rahardjo, Sinung; Utari, Heny Budi; Wiradana, Putu Angga
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.276-291

Abstract

The stress level of vannamei shrimp (Litopenaeus vannamei) is affected by increased density and several genes are expressed under the condition. This study aimed to determine the expression of genes encoding white shrimp stress after density treatment and the infectious myonecrosis virus (IMNV) challenge test. A completely randomized design (CRD) was carried out with 6 treatment groups, i.e. 3 different stocking density groups without IMNV infection (100, 200, and 400 shrimp/m2) and 3 different stocking density groups + IMNV infection (100, 200, and 400 shrimp/m2). In addition, a shrimp density of 400 shrimp/m2 reported the fastest rate of developing the IMNV virus as seen from the clinical symptoms. The lowest cumulative number of shrimp deaths was at a density of 100 shrimp/m2 and was caused by the IMNV virus confirmed through RT-PCR. Expression of stress-coding genes was divided into upregulated and downregulated characteristics. The upregulated genes were lectin and translationally controlled tumor protein (TCTP), while the downregulated gene was Toll Receptor. The results showed that the expression of genes related to immunity in L. vannamei was upregulated after pathogen challenges such as lectin and TCTP, meanwhile, the Toll receptor gene was downregulated. Further study should also be performed to measure the expression of the three genes in revealing the immune pathways.
Dry Media Formulation to Increase Productivity and Quality of Silkworms (Tubifex sp.) as A Natural Feed Development for Aquaculture Corne, Yohanes; Rahardjo, Sinung; Nurhudah, Moch.; Wiradana, Putu Angga
Jurnal Perikanan Universitas Gadjah Mada Vol 26, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.91351

Abstract

Silk worms (Tubifex sp.) are a type of natural food that is widely used for the needs of fish farming activities, especially in the freshwater fish hatchery phase. This study aimed to determine the effect of providing dried feed/media with varying doses on the productivity of silk worms. The study was conducted using a Completely Randomized Design (CRD) with three treatment groups and four replications which included administration of dry media at a dose of (P1) 150 g/m2 (P2) 250 g/m2 (P3) 350 g/m2. The initial silk worm seeds were obtained from silk worm collectors or sellers in Cipayung-East Jakarta. Maintenance of silk worms was carried out for 55 days. The results of the diversity analysis showed that there was a significant difference in the dose of dry media in cultivating silk worms (P≤0.05). The best absolute weight growth, population growth, and productivity were shown in the media treatment with a dose of 250 g/m2 (P2) with absolute biomass growth of 408.7 g, population growth of 209,381 individuals, and productivity of 1.3/m2/cycle. The results of water quality observations are still in a relatively optimal range. Silk worms have the potential to be developed as a safe, environmentally beneficial, and inexpensive natural food for cultivated animals.
Kapasitas antioksidan, sitotoksisitas dan cemaran bakteri simplisia makroalga cokelat: Antioxidant capacity, cytotoxicity, and bacterial contamination of brown macroalgae simplicia Permatasari, Anak Agung Ayu Putri Permatasari; Permatasari, Anak Agung Ayu Putri; Wiradana, Putu Angga; Sari, Ni Kadek Yunita; Widhiantara, I Gede; Rosiana, I Wayan; Sandhika, I Made Gde Sudyadnyana; Sucipto, Teguh Hari; Panjaitan, Novaria Sari Dewi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 27 No. 10 (2024): Jurnal Pengolahan Hasil Perikanan Indonesia 27(10)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v27i10.53930

Abstract

Brown algae are considered to be a source of antioxidants that can contribute to improving health. The purpose of this study was to determine the best combination of brown algae and solvent types based on the parameters of antioxidant capacity, toxicity to Vero cells, and bacterial contamination that affects brown algae simplicia during 30 d of storage. Brown algae, Sargassum aquifolium and Padina australis, were collected from Sanur Beach at the lowest point in the intertidal zone. Extraction was performed using three solvents: ethanol, methanol, and distilled water. The antioxidant capacity of the brown algae extracts was measured using the DPPH method. The brown algae extract, which showed antioxidant capacity and IC50 in the strong category, was used for cytotoxicity testing. Bacterial contamination testing was performed based on the simple storage time of brown algae for 30 d. The results showed that the combination of S. aquifolium and P. australis ethanol extract (ratio 1:10) had a yield percentage of 37.2% with an antioxidant capacity of 0.935±0.003 mg/L GAEAC and an IC50 value of 89.03 μg/mL (strong category). The combination of S. aquifolium and P. australis ethanol extracts had an IC50 value of 382.30 μg/mL (the weak category). Total plate count (TPC), Enterobacteriaceae, and coliforms increased with the storage time of simplicia for 30 days. These findings indicate that the combination of ethanol extracts of S. aquifolium and P. australis is a potential source of natural antioxidants.
Physical and molecular identification of fish and shrimp diseases in some farms of West Java, Banten and Jakarta, Indonesia Adam Saba Anggara; Ida Ayu Mirah Meliana Dewi; Kismono Kudoasmoro; Putu Eka Sudaryatma; Putu Angga Wiradana
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.157-167

Abstract

Infectious disease is a major challenge in fish and shrimp farming systems caused by microorganisms including bacteria, viruses and parasites. Physical detection has limitations in detecting diseases in cultivated animals earlier, because of the varied clinical symptoms. The aims of this study was to identify the physical and molecular presence of infectious pathogens in fish and shrimp cultivated in several ponds in West Java, Banten and Jakarta. Monitoring was carried out in two stages, namely the period March–April 2021 and September 2021 by taking samples from several ponds in Bogor, Tangerang, Depok and Jakarta. The sample criteria used were fish and shrimp showing normal and clinical symptoms of being attacked by a disease which were collected by purposive sampling. The collected samples were examined in two stages, namely physical examination of clinical symptoms and molecular examination using PCR. The results showed that of the 24 species samples collected, 17 species showed normal clinical symptoms and seven species showed clinical symptoms. Of the 24 fish and shrimp samples, four samples were infected (2 samples from normal species and 2 samples with clinical symptoms). The infected normal samples were Osphronemus goramy and Litopenaeus vannamei. The infected samples with clinical symptoms were Carassius auratus by Aeromonas salmonicida and red zebra cichlid (Metriaclima estherae) by Red Sea Bream Iridoviral Disease (RSBIV). Physical testing supported by molecular detection of aquatic animals can be an effort to manage aquaculture systems in Indonesia. Keyword: aquaculture, emerging disease, fisheries products, molecular assay ABSTRAK Penyakit infeksi adalah tantangan utama pada sistem budidaya ikan dan udang yang disebabkan oleh mikroorganisme termasuk bakteri, virus, dan parasit. Deteksi secara fisik memiliki keterbatasan dalam mengetahui lebih dini penyakit pada hewan budidaya, karena gejala klinis yang bervariasi. Tujuan dari penelitian ini adalah untuk melakukan identifikasi fisik dan molekuler keberadaan patogen infeksi pada ikan dan udang yang dibudidayakan di beberapa pertambakan di Jawa Barat, Banten, dan Jakarta. Pemantauan dilakukan dalam dua tahap yaitu periode Maret – April 2021 dan September 2021 dengan mengambil sampel dari beberapa pertambakan di Bogor, Tangerang, Depok, dan Jakarta. Kriteria sampel yang digunakan adalah ikan dan udang yang menunjukkan gejala klinis terserang oleh penyakit yang dikumpulkan secara purposive sampling. Sampel yang terkumpul diperiksa dalam dua tahap yaitu pemeriksaan fisik gejala klinis dan pemeriksaan molekuler menggunakan PCR. Hasil menunjukkan dari 24 sampel spesies yang terkumpul, sebanyak 17 spesies menunjukkan gejala klinis normal dan tujuh spesies menunjukkan gejala klinis. Dari 24 sampel ikan dan udang, terdapat empat sampel yang terinfeksi (2 sampel dari spesies normal dan 2 sampel dengan gejala klinis). Sampel normal yang terinfeksi adalah Osphronemus goramy dan Litopenaeus vannamei. Sampel yang terinfeksi dengan gejala klinis adalah Carassius auratus oleh Aeromonas salmonicida dan red zebra cichlid (Metriaclima estherae) oleh Red Sea Bream Iridoviral Disease (RSBIV). Pengujian fisik yang didukung dengan deteksi molekuler pada hewan budidaya dapat menjadi upaya manajemen sistem akuakultur di Indonesia. Kata kunci: akuakultur, emerging disease, perikanan, pengujian molekuler
Co-Authors Abd. Rahman As-syakur Adam Saba Anggara Adi Ariyanto Wibisono Adi Sofyan Ansori, Muhammad Adnorita Fandah Oktariani Afta Daniel Zato Waruwu Agoes Soegianto Agung Wahyu Permadi Agustono Agustono Anak Agung Ayu Putri Permatasari Aondohemba Samuel Nege Aprodita, Ni Wayan Seila Aris Jatmiko Aryaswari, Ni Kadek Dwi Liopita Aulia Iefan Datya Bagus Komang Satriyasa Baladrat, Nur Komariah Bambang Pontjo Priosoeryanto Bambang Yulianto Bambang Yulianto Corne, Yohanes Deny Suhermawan Yusup Dewa Ayu Angga Pebriani Dewa Ayu Intan Tirta Sari Dewi, Pande Putu Indira Prima Eka Putri Suryantari Elizabeth, Gladys Eva Harlina Fahror Rosi Fimaputra, Jatu Maranatha Fransiskus Jimmy Roga Garry Ganvis Jonteo Hani Gede Surya Indrawan Hanipa, Petronela Herdiansyah, Mochammad Aqilah I Gede Widhiantara I Ketut Wija Negara I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Saka Wijaya I Made Sara Wijana I Made Sara Wijana I Made Wisnu Adhi Putra I Wayan Rosiana Ida Ayu Mirah Meliana Dewi Juniartini, Nyoman Sweet Kismono Kudoasmoro Komang Kartika Indi Swari Kusala, Muhammad Khaliim Jati Kusala Kusuma, Nathania Eka Putri Lanniari, Nafrina Luh Putu Widiastini M. Rheza Rizki Syahputra Made Nyandra MADE PHARMAWATI Matilda, Yuliana Matius Victorino Ola Dame Mirni Lamid Moch. Nurhudah Mochammad Aqilah Herdiansyah Mohammad Taufiq Shidqi Ni Kadek Dwipayani Lestari NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Luh Sunarsih Ni Luh Watiniasih Ni Made Wagi Ambakesari Ni Putu Adriani Astiti Ni Putu Putri Wijayanti Ni Wayan Ayu Wiartini Ni Wayan Deswiniyanti Novaria Sari Dewi Panjaitan Nurhudah, Moch. Nurina, Dien Uma Nwet Darli Kyaw Zaw Okabayashi, Tamaki Panjaitan, Novaria Sari Dewi Panjaitan, Novaria Sari Dewi Panjaitan Pelupessy, Yesha Ainesis El Gracianita Permatasari , Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Prastyadi Wibawa Rahayu Putri, Fadillaisyia Riandani Putu Eka Sudaryatma Putu Eka Sudaryatma Rachmi Ridho Raden Joko Kuncoroningrat Susilo Rahardja, Viryanando Evan Rahardjo, Sinung Rahmadi Prasetijo Razaq, Imanuddin Retno Wulansari Rizhar Eman Karunia Akbar Rizqy, Aimatun Nisfia Rostantinata, Riski Sari, Ni Kadek Yunita Sari Sektiana, Sinar Pagi Setyo Budi Kurniawan Sin War Naw Sinung Rahardjo Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sugama, I Ketut Surachmi Setiyaningsih Teguh Hari Sucipto, Teguh Hari Utari, Heny Budi Viryanando Evan Rahardja Waruwu, Ernawati Wiartini, Ni Wayan Ayu Wiartini Wibisono, Adi Ariyanto Widiiastuti, Ni Komang Wijaya, I Made Saka Win Darmanto Yesha Ainensis El G. Pelupessy Yohanes Corne