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Concentrations of Heavy Metals in Three Brown Seaweed (Phaeophyta: Phaeophyceae) Collected from Tourism Area in Sanur Beach, Coast of Denpasar, Bali and Public Health Risk Assessment I Wayan Rosiana; Putu Angga Wiradana; Anak Agung Ayu Putri Permatasari; Yesha Ainensis El G. Pelupessy; Matius Victorino Ola Dame; Agoes Soegianto; Bambang Yulianto; I Gede Widhiantara
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.33103

Abstract

Highlight Research Brown seaweed heavy metals content varies between species Risk assessment showed low health risk for heavy metal from intake of the three brown seaweed The three types of brown seaweed did not show carcinogenic properties to metal Arsenic (As) Abstract Marine brown seaweed are known as one of the potential biological agents to be developed as functional food and medicinal sectors. This study aims to examine the concentration of heavy metals (Pb, Cd, Hg, and As) in brown algae (Sargassum aquifolium, Padina australis, and Turbinaria ornata.) and the possible exposure to health risks caused by consumption.  Heavy metal concentrations were determined using Atomic Absorption Spectroscopy (AAS) on brown seaweed samples obtained from three different sites. The average concentration of heavy metals in the dry weight of brown seaweed remains within the guidelines established by The Food and Drug Supervisory Agency (BPOM) Number 32 of 2019 concerning the Safety and Quality of Traditional Medicines, which is then used to calculate the estimated daily intake (EDI), target hazard quotient (THQ and TTHQ), and target cancer risk (TCR) for arsenic associated with food exposure to potentially toxic metallic elements. Each species of brown seaweed has a THQ and TTHQ level of <1, indicating that one or more toxic metal elements in the same meal provide no significant non-carcinogenic risk. The TCR for arsenic in these seaweeds are all less than 1 x 10-4, indicating no cancer risk. There are no chronic health hazards related with the ingestion of brown seaweed harvested from the coast of Sanur Beach at Denpasar, Bali.
Teknik Deteksi Cemaran Avian Influenza Virus (AIV) Pada Daging Unggas Yang Diperdagangkan Di Pasar Tradisional Di Indonesia: Mini Review Ni Made Wagi Ambakesari; Putu Angga Wiradana
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 6 (2023): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v6i.2767

Abstract

Penyakit flu burung (Avian influenza/AI) merupakan penyakit yang disebabkan oleh virus Avian influenza dari famili Orthomyxoviridae, yang bersifat zoonosis dan mempunyai material genetik berupa RNA berpolaritas negatif, dengan diameter 120 nm dan strukturnya berfilamen. Secara klinis, unggas yang telah terinfeksi virus AI menunjukan gejala gangguan sistem neurologis seperti tortikolis, tremor, kesulitan berdiri, kehilangan keseimbangan, dan hingga kematian unggas. Polymerase Chain Reaction (PCR) merupakan salah satu metode enzimatis untuk melipatgandakan (amplification) secara eksponensial suatu sekuen nukleotida tertentu secara in vitro. Teknik PCR sangat umum digunakan dalam deteksi keberaaan virus AI secara dini dan waktu yang relatif singkat. Namun, masih terdapat teknik lainnya yang dikembangkan untuk mempermudah pelacakan virus ini terutama pada lingkungan pasar tradisional yang masih terbatas dilaporkan. Penelitian ini bertujuan untuk mengetahui metode deteksi virus flu burung beserta subtipenya pada daging unggas yang dijual di pasar tradisional di Indonesia. Telaah singkat ini akan mengungkapkan penelitian-penelitian terkait mengenai sebaran virus flu burung dan metode deteksi yang digunakan terutama pada daging unggas yang telah dijual di pasar tradisional. Pengumpulan data diakses pada Google Scholar, PubMed, Scopus, Web of Science (WoS), dan Lembaga pengindeks nasional/internasional lainnya yang diterbitkan dalam kurun waktu 10 tahun terakhir. Telaah singkat ini sangat penting dilakukan untuk memberikan informasi yang komprehensif mengenai teknik yang umum digunakan dalam upaya deteksi penyebaran virus AI, terutama pada daging unggas yang dijual di pasar tradisional serta meningkatkan kewaspadaan melalui deteksi dini terhadap penyebaran virus AI di masa mendatang.
Tinjauan Perbandingan Evektifitas Pemeriksaan Histopatologi Dan FAT (Fluorescent Antibody Test) Pada Virus Rabies: Artikel Review Garry Ganvis Jonteo Hani; Afta Daniel Zato Waruwu; Putu Angga Wiradana; I Gede Widhiantara
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 6 (2023): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v6i.2863

Abstract

Virus rabies merupakan patogen zoonotik yang menyebabkan penyakit serius padamamalia, termasuk manusia. Identifikasi cepat dan akurat dari virus ini penting untukmanajemen kasus dan pencegahan penularan. Tinjauan ini mengevaluasi efektivitas duametode diagnostik utama, yaitu pemeriksaan histopatologi dan Fluorescent Antibody Test(FAT), dalam mendeteksi virus rabies. Pemeriksaan histopatologi, yang melibatkan analisisstruktur sel dan jaringan secara mikroskopis, telah lama menjadi standar dalam diagnosisvirus rabies Namun, kelebihan waktu yang diperlukan untuk memproses sampel dankompleksitas prosedurnya dapat menjadi kendala. FAT menggunakan antibodi fluoresen untukmendeteksi antigen virus rabies secara langsung dalam jaringan yang terinfeksi. Metode inidikenal karena kecepatan hasilnya, tetapi kemungkinan adanya hasil palsu positif dan negatifmemerlukan peninjauan kritis. Peninjauan ini memberikan wawasan mendalam tentangperkembangan terkini dalam diagnosis virus rabies dan memberikan dasar untuk pemilihanmetode diagnostik yang paling efektif dalam konteks penelitian dan pengelolaan penyakit ini.Hasil menunjukan bahwa pemeriksaan FAT lebih efektif karena Metode ini dapat memberikanhasil dalam waktu yang relatif singkat dan Proses FAT lebih sederhana serta peralatan yanglebih mudah diakses dibandingkan Histopatologi.
PKM PENDAMPINGAN STANDARISASI BAHANALAMBAGIGURU DAN SISWA SMK KESEHATAN BALI MEDIKA, DENPASAR I Made Gde Sudyadnyana Sandhika; Ni Kadek Yunita Sari; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Wayan Rosiana; Putu Angga Wiradana; I Made Wisnu Adhi Putra; Ni Kadek Dwipayani Lestari; Rahmadi Prasetijo; I Made Murna
Seminar Nasional Aplikasi Iptek (SINAPTEK) Vol. 6 (2023): PROSIDING SINAPTEK
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pengetahuan dan keterampilan terkait proses standarisasi bahan alamdi SMKKesehatan Bali Medika kurang diperhatikan karena kurikulumnya lebih banyak memuat matapelajaran produktif keperawatan, teknik laboratorium medik dan farmasi. Sebagai salahsatusekolah swasta di Kota Denpasar, sekolah ini masih memiliki alat dan bahan penunjangpraktikum di bidang Sains yang masih sangat terbatas. Konsep dasar standarisasi bahanalam termasuk penting diberikan di Sekolah ini mengingat sekolah ini merupakan sekolahkesehatan dan memiliki jurusan farmasi. Keterampilan standarisasi bahan alamberguna bagisiswa pada saat magang di dunia industri dan pada saat melanjutkan ke jenjang pendidikanyang lebih tinggi. Tujuan dari kegiatan pengabdian masyarakat ini yaitu: 1)Memberikanwawasan mengenai standarisasi meliputi jenis standarisasi, fungsi standarisasi dan metodestandarisasi pada bahan alam khususnya tanaman obat, 2)Memberikan pengetahuanmengenai teknik-teknik standarisasi baik secara spesifik dan non spesifik pada bahan alamkhususnya tanaman obat, dan 3)Mempraktekan secara langsung dalam membuat sediaanekstrak yang berbahan dasar tanaman obat. Hasil dari Pengabdian Masyarakat ini adalah1)Wawasan mitra terkait standarisasi bahan alam meningkat dari rata-rata nilai 55 menjadi86 setelah posttest, 2)Tingkat pengetahuan mitra meningkat dari tidak tahu menjadi tahuakan teknik-teknik standarisasi dan semua anggota mitra yang mengikuti sosialisasimendapatkan rerata nilai pemahaman 84 pada post-test, 3)Penyampaian materi pembuatansediaan ekstrak bahan alam khususnya tanaman obat meningkat dari rata-rata nilai 45 padapretest menjadi 83 setelah posttest.
Penempelan Teritip (Arthropoda) pada Beberapa Jenis Mangrove di Kawasan TAHURA Ngurah Rai Bali Juniartini, Nyoman Sweet; Watiniasih, Ni Luh; Astiti, Ni Putu Adriani; Wiradana, Putu Angga
Bumi Lestari Journal of Environment Vol 24 No 1 (2024)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2024.v24.i01.p01

Abstract

Mangrove ecosystems are able to support biological and ecological life cycles and provide welfare for coastal communities. Pest outbreaks that attack seedlings to mangrove trees often cause ecological losses throughout the TAHURA Ngurah Rai Bali area. The ecological interactions of barnacle pest species in mangrove ecosystems are very complex and highly detailed studies. There is empirical evidence that the attachment of barnacles is strongly associated with allelochemical compounds produced by mangroves. This study aims to inventory the attachment of barnacles (arthropods) to several types of mangroves in the TAHURA Ngurah Rai area of Bali. This study used a purposive sampling method to pay for the location of the observations. Quadrant transects were used to organize the classification of the mangrove plots, barnacle densities being calculated for each transect. The density of barnacles was calculated on the number of barnacles attached to the mangrove plants, then recorded the types of plants attached, types of barnacles, types of planting and the number of individuals for each mangrove classification. The study showed that barnacles planted most of the mangrove seedlings at all stations, while the classification of tree plots only had barnacles attached at station 3. The number of attachments of Amphibalanus sp. the highest was found at station 3 of the R. mucronata tiller classification of 881 individuals, while no attachment of barnacles was found at all stations for S. alba mangroves. Overall, the attachment of barnacles was higher in R. mucronata tillers and had the potential to reduce their growth productivity in the field. Efforts to control barnacles really need to be done to increase the success of planting mangroves in TAHURA Ngurah Rai, Bali.
Status, Trends, and Potentials of Turtle Conservation in Bali: A Mini Review Pelupessy, Yesha Ainesis El Gracianita; Wiradana, Putu Angga; Rosiana, I Wayan; Widhiantara, I Gede
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.256-268

Abstract

Sea turtles in Bali has been exploited excessively, which caused decrease in population, and this issue has placed sea turtles threatened to extinction. This review article aimed to provide information regarding the status, trends, and potential of sea turtles in Bali. Information given in this article is vital to ensure reliable knowledge not to only understand our current situation, but also to increase efficiency in reliant to the problem sea turtles face. The literature study method is used to write this review paper, namely by accessing a number of research paper published nationally and internationally. It was explained that the status of sea turtles is known to be endangered and law enforcement is not sufficient overcoming the problem. Conservation trends such as nurturing hatchlings have shown to not only help restore the population of sea turtles, but also to bring economic benefits to the conservation sites and the people. Potentials of ecotourism and DNA Barcoding has shown to be effective to benefit the people economically and increase the efficiency of law enforcement and conservation. Solutions and methods of improvement such as ecotourism and DNA Barcoding explained in this article is practical for Bali to adapt, so that sea turtle conservation is capable to overcome its status and incline to its potential.
Contaminants and Human Health Risks Associated with Exposure to Microplastic Ingestion of Green Mussels (Perna viridis) Collected from The Kedonganan Fish Market, Bali Wiradana, Putu Angga; Sandhika, I Made Gde Sudyadnyana; Widhiantara, I Gede; Rizqy, Aimatun Nisfia; Soegianto, Agoes; Yulianto, Bambang
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.197-208

Abstract

Microplastics (MPs) are pollutant agents that have been absorbed and detected in aquatic ecosystems at high concentrations. This study aimed to investigate the presence of MPs pollution in green mussel (Perna viridis) products sold at the Kedonganan fish market, Badung, Bali. A total of 150 mussels with an average weight of 3,2 ± 0,71 g/mussels from three traders each composed and followed by the pre-treatment stage using 5 M NaCl solution, extraction with wet oxidation peroxidation (WPO) + Fe(II) catalyst and filtered. The highest percentage for the form of MPs was successively obtained by the Line form in Trader A at 85,42% and the lowest in Trader C at 50,00%. The highest form of fragments was obtained in Trader C at 42,86%. Film and filament forms were only obtained in Trader A. The highest MPs color was black and the lowest was gray. The highest average MPs particle size was found in the form of a filament of 1944,37 ± 88,41 μm which was found in Trader A. Estimates of MPs intake per year/capita in Indonesia showed that exposure to MPs through consumption of green mussels in this study amounted to 498,330 MPs/year/capita items. Overall, the green mussel from Trader A had the highest percentage and size of MPs, with the shape of fragment MPs being dominated by Trader C and the color of the MPs being dominated by black. MPs exposure to green mussel consumption in Indonesia is very high, but no health impact category has yet been found for this estimate.
Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV) Nurhudah, Moch.; Baladrat, Nur Komariah; Rahardjo, Sinung; Utari, Heny Budi; Wiradana, Putu Angga
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.276-291

Abstract

The stress level of vannamei shrimp (Litopenaeus vannamei) is affected by increased density and several genes are expressed under the condition. This study aimed to determine the expression of genes encoding white shrimp stress after density treatment and the infectious myonecrosis virus (IMNV) challenge test. A completely randomized design (CRD) was carried out with 6 treatment groups, i.e. 3 different stocking density groups without IMNV infection (100, 200, and 400 shrimp/m2) and 3 different stocking density groups + IMNV infection (100, 200, and 400 shrimp/m2). In addition, a shrimp density of 400 shrimp/m2 reported the fastest rate of developing the IMNV virus as seen from the clinical symptoms. The lowest cumulative number of shrimp deaths was at a density of 100 shrimp/m2 and was caused by the IMNV virus confirmed through RT-PCR. Expression of stress-coding genes was divided into upregulated and downregulated characteristics. The upregulated genes were lectin and translationally controlled tumor protein (TCTP), while the downregulated gene was Toll Receptor. The results showed that the expression of genes related to immunity in L. vannamei was upregulated after pathogen challenges such as lectin and TCTP, meanwhile, the Toll receptor gene was downregulated. Further study should also be performed to measure the expression of the three genes in revealing the immune pathways.
The Role of Sembung (Blumea balsamifera) Leaf Extract in Preventing Atherosclerosis in Hyperlipidemia Rat Models I Made Jawi; I Wayan Putu Sutirta Yasa; I Gede Widhiantara; Anak Agung Ngurah Subawa; Putu Angga Wiradana; Naw, Sin War
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.6673

Abstract

This study aims to prove that administering BBLE as a natural antioxidant can prevent atherosclerosis by maintaining lipid profiles, antioxidant enzymes, and netrin-1 levels in hyperlipidemia in rat models. The research subjects were 20 adult male Wistar rats (Rattus norvegicus), which were divided into 2 groups using a randomized pretest and posttest control group design. Before treatment and after treatment for 3 months, lipid profiles, MDA, SOD, and netrin-1  were examined. The control group was only given high-cholesterol diets (HCD), while the treatment, apart from HCD, was also given BBLE 4mg/day. The data obtained was tested using paired t-test and group t-test. The results of the study showed that there was a significant decrease in netrin-1 in the control group (p<0.05) after being given HCD for three months. In the treatment group, it also decreased but it was not significant (p>0.05). Netrin-1 levels in the treatment group were higher than the control (p<0.05). The lipid profile experienced a significant increase in HDL in the treatment group accompanied by a significant decrease in MDA and an increase in SOD (p<0.05) when compared with the control group. This study concludes that administering BBLE at a dose of 4 mg/day to rats given HCD caused an increase in netrin-1 levels accompanied by improvements in lipid profiles and prevention of oxidative stress. The findings of this study reveal the novelty of BBLE in treating and maintaining blood vessel function in mice given HCD by increasing netrin-1 levels.
Dry Media Formulation to Increase Productivity and Quality of Silkworms (Tubifex sp.) as A Natural Feed Development for Aquaculture Corne, Yohanes; Rahardjo, Sinung; Nurhudah, Moch.; Wiradana, Putu Angga
Jurnal Perikanan Universitas Gadjah Mada Vol 26, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.91351

Abstract

Silk worms (Tubifex sp.) are a type of natural food that is widely used for the needs of fish farming activities, especially in the freshwater fish hatchery phase. This study aimed to determine the effect of providing dried feed/media with varying doses on the productivity of silk worms. The study was conducted using a Completely Randomized Design (CRD) with three treatment groups and four replications which included administration of dry media at a dose of (P1) 150 g/m2 (P2) 250 g/m2 (P3) 350 g/m2. The initial silk worm seeds were obtained from silk worm collectors or sellers in Cipayung-East Jakarta. Maintenance of silk worms was carried out for 55 days. The results of the diversity analysis showed that there was a significant difference in the dose of dry media in cultivating silk worms (P≤0.05). The best absolute weight growth, population growth, and productivity were shown in the media treatment with a dose of 250 g/m2 (P2) with absolute biomass growth of 408.7 g, population growth of 209,381 individuals, and productivity of 1.3/m2/cycle. The results of water quality observations are still in a relatively optimal range. Silk worms have the potential to be developed as a safe, environmentally beneficial, and inexpensive natural food for cultivated animals.
Co-Authors Abd. Rahman As-syakur Adam Saba Anggara Adi Ariyanto Wibisono Adi Sofyan Ansori, Muhammad Adnorita Fandah Oktariani Afta Daniel Zato Waruwu Agoes Soegianto Agung Wahyu Permadi Agustono Agustono Anak Agung Ayu Putri Permatasari Anak Agung Ngurah Subawa Aondohemba Samuel Nege Aris Jatmiko Aulia Iefan Datya Bagus Komang Satriyasa Baladrat, Nur Komariah Bambang Pontjo Priosoeryanto Bambang Yulianto Bambang Yulianto Corne, Yohanes Deny Suhermawan Yusup Dewa Ayu Angga Pebriani Dewa Ayu Intan Tirta Sari Dewi, Pande Putu Indira Prima Eka Putri Suryantari Eva Harlina Fahror Rosi Fimaputra, Jatu Maranatha Fransiskus Jimmy Roga Garry Ganvis Jonteo Hani Gede Surya Indrawan Hanipa, Petronela I Gede Widhiantara I Ketut Wija Negara I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Saka Wijaya I Made Sara Wijana I Made Sara Wijana I Made Wisnu Adhi Putra I Wayan Putu Sutirta Yasa I Wayan Rosiana Ida Ayu Mirah Meliana Dewi Juniartini, Nyoman Sweet Kismono Kudoasmoro Komang Kartika Indi Swari Kusuma, Nathania Eka Putri Lanniari, Nafrina Luh Putu Widiastini M. Rheza Rizki Syahputra Made Nyandra MADE PHARMAWATI Matilda, Yuliana Matius Victorino Ola Dame Mirni Lamid Moch. Nurhudah Muhammad Taufiq Shidqi Naw, Sin War Ni Kadek Dwipayani Lestari NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Luh Sunarsih Ni Luh Watiniasih Ni Made Wagi Ambakesari Ni Putu Adriani Astiti Ni Putu Putri Wijayanti Ni Wayan Ayu Wiartini Ni Wayan Deswiniyanti Nurhudah, Moch. Nurina, Dien Uma Nwet Darli Kyaw Zaw Okabayashi, Tamaki Panjaitan, Novaria Sari Dewi Pelupessy, Yesha Ainesis El Gracianita Permatasari , Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Prastyadi Wibawa Rahayu Putri, Fadillaisyia Riandani Putu Eka Sudaryatma Putu Eka Sudaryatma Rachmi Ridho Raden Joko Kuncoroningrat Susilo Rahardja, Viryanando Evan Rahardjo, Sinung Rahmadi Prasetijo Razaq, Imanuddin Retno Wulansari Rizhar Eman Karunia Akbar Rizqy, Aimatun Nisfia Rostantinata, Riski Sektiana, Sinar Pagi Setyo Budi Kurniawan Sin War Naw Sinung Rahardjo Sugama, I Ketut Surachmi Setiyaningsih Teguh Hari Sucipto, Teguh Hari Utari, Heny Budi Viryanando Evan Rahardja Waruwu, Ernawati Wibisono, Adi Ariyanto Widiiastuti, Ni Komang Wijaya, I Made Saka Yesha Ainensis El G. Pelupessy Yohanes Corne