Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

3.457

P-Index

This Author published in this journals

All Journal Medical Journal of Indonesia The Indonesian Biomedical Journal Sari Pediatri Paediatrica Indonesiana Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Molecular and Cellular Biomedical Sciences (MCBS) Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) GERVASI: Jurnal Pengabdian kepada Masyarakat JFIOnline Jurnal Ilmu Kefarmasian Indonesia Pharmaceutical Journal of Indonesia Jurnal Jamu Indonesia Hubungan Tingkat Pengetahuan Petugas Pengelola Obat dengan Tingkat Ketersediaan Obat Di Puskesmas Kota Malang Narra J Cermin Dunia Kedokteran eJournal Kedokteran Indonesia Journal of Health and Nutrition Research Makara Journal of Health Research Molekul: Jurnal Ilmiah Kimia Journal of The Indonesian Society of Integrated Chemistry Jurnal Kefarmasian Indonesia Indonesian Journal of Jamu Cermin Dunia Kedokteran

Melva Louisa

Departement Of Pharmacology And Therapeutics, Faculty Of Medicine, Universitas Indonesia, Jakarta

Author-ID : 308840

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

Published : 45 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4 5

Incretin-based therapies for type 2 diabetes mellitus in Asian patients: Analysis of clinical trials Louisa, Melva; Takeuchi, Madoka; Takeuchi, Masahiro; Nafrialdi, Nafrialdi; Setiabudy, Rianto
Medical Journal of Indonesia Vol 19, No 3 (2010): August
Publisher : Faculty of Medicine Universitas Indonesia

Aim To review the effi cacy and safety data on incretin-based therapies currently available (exenatide, liraglutide, sitagliptin, vildagliptin) for the treatment of type 2 diabetes mellitus in Asian population.Methods We conducted Medline search of all relevant randomized clinical trials of incretin-based therapies for type 2 diabetes mellitus in Asian populations. Data pertinent to the efficacy and safety of GLP-1 mimetics and DPP-4 inhibitors were extracted and used.Results We found 14 randomized controlled trials of incretin based-therapy which included 3567 type 2 diabetes mellitus in Asian population (Japanese, Chinese, Korean, Indian). It was shown that incretin-based therapies improved HbA1c at higher extent (up to -1.42% in exenatide 10 mcg bid, -1.85% for liraglutide 0.9 mg qd, -1.4% for sitagliptin 100 mg and -1.4% for vildagliptin 50 mg bid) compared to the effects observed in studies with Caucasian population, with comparable safety profile.Conclusion The efficacy of incretin-based therapies in Asian patients improved glycemic parameters in a higher magnitude on some glycemic parameters compared with those in Caucasian population. These results indicate that incretin-based therapies may be more effective in Asian population than in Caucasian. (Med J Indones 2010; 19: 205-12)Key words: exenatide, incretin, liraglutide, sitagliptin, type-2 diabetes, vildagliptin

Influence of primaquine and ritonavir interaction on CYP3A4 mRNA expression in HepG2 cell culture Iskandarmudasyah, Adam; Louisa, Melva; Arleni, Arleni; Jusman, Sri W.A.; Suyatna, Franciscus D.
Medical Journal of Indonesia Vol 21, No 1 (2012): February
Publisher : Faculty of Medicine Universitas Indonesia

Background: Concomitant treatment with antimalaria and antiretroviral drug is a new challenge in the management of malaria and HIV co-infection. Primaquine is a substrate and also an inhibitor of CYP3A4, while ritonavir is a substrate, an inhibitor, and also an inducer for CYP3A4. The objective of this study is to measure the CYP3A4 mRNA expression in HepG2 cell culture induced by primaquine and ritonavir co-treatment.Methods: For the initial study HepG2 cells were treated with 30, 40, 50 uM of primaquine; 2, 10, 20 uM ritonavir; DMSO â¤0.1 % for negative control; or 20 uM rifampicin for positive control. While for the co-treatment study the cells were treated with 40 uM primaquine+10 uM ritonavir; DMSO â¤0.1 %; or 20 uM rifampicin for 72 hours. The cells were harvested using trypsinâEDTA and total RNA was extracted using the Tripure isolation reagent. After determining the quantity of RNA spectrophotometrically, CYP3A4 mRNA expression was quantified using real-time reverse transcription polymerase chain reaction (RT-PCR).Results: The expression of CYP3A4 mRNA was up-regulated (1.22 fold over control) in HepG2 cells co-treated with primaquine and ritonavir. These data suggest that the induction effect of ritonavir was more dominant than the inhibitory effect of primaquine.Conclusion: Concomitant administration of primaquine and ritonavir result in up-regulation of CYP3A4 mRNA expression in vitro. (Med J Indones 2012;21:3-7)Keywords: CYP450 induction, CYP3A4, drug interaction, primaquine, ritonavir

Primaquine decreased plasma concentration of ritonavir: single- and repeated-dose study in Sprague Dawley rats Louisa, Melva; Soetikno, Vivian; Nafrialdi, Nafrialdi; Setiabudy, Rianto; Suyatna, Frans D.
Medical Journal of Indonesia Vol 20, No 3 (2011): August
Publisher : Faculty of Medicine Universitas Indonesia

Background: The present study was aimed to explore the effects of ritonavir and primaquine combination given as a singledoseÂ or repeated-dose compared to ritonavir alone on ritonavir plasma concentration in the rats.Methods: In single-dose study, 30 male Spraque Dawley rats were randomly allocated to receive ritonavir 20 mg/kg BW orÂ ritonavir 20 mg/kg BW + primaquine 1.2 mg/kg BW or ritonavir 20 mg/kg BW + ketokonazole 10 mg/kg BW. KetokonazoleÂ was used as positive control of ritonavir metabolism inhibitor. In the repeated-dose study, thirty Spraque Dawley male ratsÂ were randomly allocated to receive ritonavir 20 mg/kg BW/day or ritonavir 20 mg/kg BW/day + primaquine 1.2 mg/kg BW/day or ritonavir 20 mg/kg BW/day + rifampicin 100 mg/kg BW/day. Rifampicin was used as a positive control of ritonavirÂ metabolism inducer.Results: In the single-dose study, ketokonazole increased the area under the plasma concentration (AUC) of ritonavir (â114.8%, p< 0.05), while primaquine tended to decrease the AUC of ritonavir (â 32.6%, p> 0.05). Repeated-dose studyÂ showed that rifampicin decreases the AUC of ritonavir (â 42.8%, p< 0.001), and primaquine decreased the AUC of ritonavirÂ plasma concentration (â 46.6%, p< 0.001).Conclusion: Concomitant administration of primaquine and ritonavir decreases the AUC of ritonavir. This effect may resultÂ in the insufficient concentration of ritonavir as anti-HIV, which may lead to treatment failure with ritonavir. (Med J IndonesÂ 2011; 20:190-4)Keywords: drug interaction, metabolism, primaquine, ritonavir

The effect of lycopene on the total cytochrome P450, CYP1A2 and CYP2E1 Louisa, Melva; Suyatna, Frans D.; Setiawati, Arini; Jusman, Sri W.A.
Medical Journal of Indonesia Vol 18, No 4 (2009): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Aim: Some carotenoids such as canthaxantin, astaxanthin and beta apo-8â-carotenal were reported to have modulatoryeffect on the cytochrome P450. The present study was conducted to investigate the effects of lycopene, a nonprovitamin A carotenoid, on microsomal cytochrome P450, CYP1A2 and CYP2E1.Methods: Total cytochrome P450 levels, CYP1A2 and CYP2E1-catalyzed reactions (acetanilide 4-hydroxylation and p-nitrophenol hydroxylation) were studied in the liver microsomes of male Sprague Dawley rats. Microsomes were prepared using differential centrifugation combined with calcium aggregation method. Lycopene was orally administered in the dosages of 0, 25, 50 or 100 mg/kgBW/day for 14 days in a repeated fashion. Data were analyzed using ANOVA test.Results: Total cytochrome P450 level and acetanilide 4-hydroxylase activity were unaffected by any of the treatments. The CYP2E1 probe enzyme (p-nitrophenol hydroxylase) was significantly reduced by repeated administration of 100mg/ kgBW/day lycopene (7.88 + 2.04 vs 12.26 + 2.77 n mol/min/mg prot).Conclusion: The present results suggest that lycopene does not affect the total cytochrome P450 or CYP1A2 activity but it inhibits the activity of CYP2E1 (p-nitrophenol hydroxylase) in the rat. (Med J Indones 2009; 18: 233-8)Keywords: lycopene, cytochrome P450, CYP1A2, CYP2E1

A closer look at hypoxia inducible factor Louisa, Melva
Medical Journal of Indonesia Vol 21, No 3 (2012): August
Publisher : Faculty of Medicine Universitas Indonesia

[No Abstract Available]

Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatment Paramita, Paramita; Louisa, Melva; Nafrialdi, Nafrialdi
Medical Journal of Indonesia Vol 25, No 4 (2016): December
Publisher : Faculty of Medicine Universitas Indonesia

Background: Epithelial mesenchymal transition (EMT) plays a significant role in the development of cancer cell resistance to drugs. Vimentin, a type III intermediate filament protein, is a marker of EMT. Vimentin's over-expression in cancer correlates well with increased tumor growth, change in cell shape and poor prognosis. Endoxifen is an active metabolite of tamoxifen and has become a new potent agent in the treatment of breast cancer. This is a study that aimed to investigate the effect of endoxifen exposure with or without estradiol on cell viability, cell morphology and EMT progression through the analysis of vimentin mRNA expression after 4-week treatment.Methods: Endoxifen, 100 nM or 1,000 nM, with or without beta-estradiol were given repeatedly to MCF-7 cells. Cells treated with dimethyl sulfoxide (DMSO) 0.001% were used as control. After 2- and 4-week exposure, the cells were counted, analyzed for mRNA vimentin expression, and observed for morphological changes.Results: Compared to control, there were significant decreases in vimentin mRNA expressions in endoxifen and endoxifen+β-estradiol treated cells after 2-weeks, which then significantly increased after 4-week compared with the 2-week exposure. We found no change in morphology of MCF-7 cells.Conclusion: Repeated exposure of endoxifen might induce EMT progression through increased expression of vimentin in MCF-7 breast cancer cell line.

The effects of quercetin on oxidative stress and fibrosis markers in chronic kidney disease rat model Layal, Kamalia; Perdhana, Ika S.; Louisa, Melva; Estuningtyas, Ari; Soetikno, Vivian
Medical Journal of Indonesia Vol 26, No 3 (2017): September
Publisher : Faculty of Medicine Universitas Indonesia

Background: Oxidative stress may play a role in the pathogenesis of (CKD), Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor involved in cell defense mechanism against oxidative stress. In this study, we examined the effect of quercetin, a polyphenplic antioxidant anti fibrosis compund in fruits and vegetables, on the 5/6 nephrectomy-induced CKD progression model rats through modulation of Nrf2 expression.Methods: Male Sprague-Dawley rats were randomly divided into normal control group (C), untreated 5/6 nephrectomy (Nx), quercetin-treated 5/6 nephrectomy (100 mg/kgBW/day orally) (NxQ), and captopril-treated 5/6 nephrectomy (10 mg/kgBW/day orally) (NxK) for 8 weeks. At the end of study, all animals were sacrified. Urine, blood, and kidney tissues were taken for examination of proteinuria, plasma creatinine, urea, malondialdehyde (MDA), glutathione peroxidase (GPx) activity, Nrf2, Keap1, heme oxygenase-1 (HO-1) expressions, and renal fibrosis.Results: Quercetin administration did not affect the level of protein in urine, plasma creatinine, and urea. However, it tended to reduce the level of MDA, increase GPx activity, Nrf2, Keap1, and HO-1 expression as well as the degree of fibrosis.Conclusion: In 5/6 nephrectomized rats, quercetin tended to ameliorate the level of MDA, GPx activity, Nrf2, Keap1, and HO-1 expression. In addition, quercetin tended to decrease the degree of fibrosis in the remnant kidney.

Medicinal plants: source of new lead compounds in therapeutics Louisa, Melva
Medical Journal of Indonesia Vol 22, No 3 (2013): August
Publisher : Faculty of Medicine Universitas Indonesia

[no abstract available]

Effective and safe pharmacotherapy for pediatric population: a call for academia to conduct clinical research Louisa, Melva
Medical Journal of Indonesia Vol 27, No 4 (2018): December
Publisher : Faculty of Medicine Universitas Indonesia

[no abstract available]

Knock-out transmembrane prostate androgen-induced protein gene suppressed triple-negative breast cancer cell proliferation Wardhani, Bantari W.K.; Puteri, Meidi U.; Watanabe, Yukihide; Louisa, Melva; Setiabudy, Rianto; Kato, Mitsuyasu
Medical Journal of Indonesia Vol 26, No 3 (2017): September
Publisher : Faculty of Medicine Universitas Indonesia

Background: Triple negative breast cancer (TNBC) tends to grow more rapidly and has poorer prognosis compared to others. High expression of transmembrane prostate androgen-induced protein (TMEPAI) correlates with poor prognosis in TNBC patients. However, the mechanistic role of TMEPAI in tumorigenic remains unknown. This study aimed to knock-out TMEPAI in TNBC cell line to determine its function further in cells proliferation.Methods: CRISPR-Cas9 has been used previously to knock-out TMEPAI in Hs857T TNBC cell line. Hs587T TNBC parental cell line (wild-type/WT) and TMEPAI knock out Hs 586T cell lines were cultured in Dulbecco’s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin and amphotericin B. Both cell lines were seeded in 24-well plates and counted every two days, then proliferation rates were plotted. Afterwards, total RNA were isolated from the cells and Ki-67, and TGF-β mRNA expression levels as proliferation markers were determined.Results: Cell proliferation rates as displayed in growth curve plots showed that WT-TMEPAI cell line grew more rapidly than KO-TMEPAI. In accordance, mRNA expression levels of Ki-67 and TGF-β were significantly decreased KO-TMEPAI as compare to TMEPAI-WT.Conclusion: Knock-out of TMEPAI attenuates cell proliferation in TNBC.

Co-Authors -, Paramita Achmad, Fariz Adam Iskandarmudasyah Adhayati, Baety Adisti Dwijayanti Agnes Frethernety Agus D. Susanto Ahmad Aulia Ali Sodikin Amanah Amanah Anang Endaryanto Andita Fitri Mutiara Rizki, Andita Fitri Mutiara Angelica Riadi Alim Saputro Angelina Riadi Alim Saputro Anom Bowo Laksono Antarianto, Radiana Dhewayani Anton Bahtiar Anton Bahtiar Ari Estuningtyas, Ari Aria Kekalih Arini Setiawati Arini Setiawati Arleni Arleni Aurelia Demtari Tuah Ayu Suraduhita Azmi, Wihda Aisarul Barasila, Atikah Chalida Budi Setiabudiawan Budi Wiweko Clara Riski Amanda Deni Rahmat Dewi, Yulia Ratna Didi Haryadi Didi Haryadi Dina Muktiarti, Dina Elisna Syahruddin Elly Yanah Arwanih Elvira Yunita Erlina Santoso Erni H. Purwaningsih Eziefule, Oluebube Magnificient Fajri, Purnama Fasha, Iqbal Franciscus D. Suyatna Frans D. Suyatna Gayatri, Anggi Gigih Andy Putra, Achmad Ginting, Tribowo T. Halim, Kelvin Handayani, Nur Hayati Dwi HANS-JOACHIM FREISLEBEN I MADE ARTIKA Indah D Dewijanti Johannes Hudyono Kato, Mitsuyasu Ketut Dewi Kumara Wati Khariri Khariri Layal, Kamalia Madoka Takeuchi Manggiasih Dwiayu Larasati Masahiro Takeuchi Mega Putri Utami Mila Maidarti Mizuno, Seiya Muhaimin Muhaimin Mulyadi Djer Nabillah, Deya Adiby Nafrialdi Nafrialdi Nasrun, Martina WS. Ni Made Dwi Sandhiutami Nihayah, Silviatun Nining Handayani Novi S. Hardiany Novita, Rachma Nur Hayati Dwi Handayani Pandelaki, Jacub Paramita - Paramita - Paramita, Paramita Pawitan, Jeanne Adiwinata Perdhana, Ika S. Puspita E. Wuyung Puspita Eka Wuyung Puspita Eka Wuyung Puteri, Meidi U. Putrihamidah, Diyanthie Aulia Rachma Novita Rachma Novita Rachman, Andika Raymond R. Tjandrawinata Rebecca Noerjani Angka Resda Akhra Syahrani Rianto Setiabudy Rianyta Rianyta Safitri, Yolanda Sake Juli Martina SEKAR ARUMSARI, SEKAR Septelia I. Wanandi Septelia Inawati Wanandi SEPTELIA INAWATI WANANDI Setiabudy, Rahajuningsih D Shafilla Yunilma Andriany Sianipar, Erlia Anggrainy Sianipar, Erlia Anggrainy Siregar, Josephine Elizabeth Siste, Kristiana Sri W.A. Jusman Sumadiono Sumadiono Suratih, Ni Made Desy Syahrani, Resda A. Syarifah Dewi Tarigan, Immanuel N. Tri Yuliani Trishna, Alya R. Ungke Antonjaya Utami, Diah S. Vivian Soetikno Vivian Soetikno Vivian Soetikno Wahyunia Likhayati Septiana Wardhani, Bantari W.K. Watanabe, Yukihide Watanabe, Yukihide Wawaimuli Arozal Wawaimuli Arozal Wawaimuli Arozal Yolanda Safitri Yolanda Safitri Zakiudin Munasir

Title

Found 45 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 45 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 45 Documents
Search