Article Per Year (5 Year)
p-Index From 2021 - 2026
3.426
P-Index
This Author published in this journals
All Journal The Indonesian Biomedical Journal Sari Pediatri Paediatrica Indonesiana Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Molecular and Cellular Biomedical Sciences (MCBS) Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) GERVASI: Jurnal Pengabdian kepada Masyarakat JFIOnline Jurnal Ilmu Kefarmasian Indonesia Pharmaceutical Journal of Indonesia Jurnal Jamu Indonesia Hubungan Tingkat Pengetahuan Petugas Pengelola Obat dengan Tingkat Ketersediaan Obat Di Puskesmas Kota Malang Narra J Cermin Dunia Kedokteran JKKI : Jurnal Kedokteran dan Kesehatan Indonesia eJournal Kedokteran Indonesia Journal of Health and Nutrition Research Makara Journal of Health Research Sciences of Pharmacy Molekul: Jurnal Ilmiah Kimia Journal of The Indonesian Society of Integrated Chemistry Jurnal Kefarmasian Indonesia Indonesian Journal of Jamu Cermin Dunia Kedokteran
Melva Louisa
Departement Of Pharmacology And Therapeutics, Faculty Of Medicine, Universitas Indonesia, Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

Published : 38 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : JFIOnline

 1 
Kurkumin Meningkatkan Sensitivitas Sel Kanker Payudara terhadap Tamoksifen melalui Penghambatan Ekspresi P-glikoprotein dan Breast Cancer Resistance Protein Sianipar, Erlia Anggrainy; Louisa, Melva; Wanandi, Septelia Inawati
Jurnal Farmasi Indonesia Vol 9, No 2 (2017)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v9i2.578

Abstract

Background:The decreased of sensitivity or resistance to tamoxifen occured after long-term treatment in breast cancer. One of the major factor in tamoxifen resistance is over expression of efflux transporter P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP). Curcumin has known as inhibitor of P-gp and BCRP. The addition of curcumin to the tamoxifen resistant cells is expected to increase the sensitivity of breast cancer cells to tamoxifen. Method:MCF-7 breast cancer cell linewas induced with tamoxifen 1 uM for 10 passage (MCF-7(T)), then cell viability and mRNA expression of P-gp and BCRP were analyzed. To the MCF- 7(T) cells, curcumin was given at of 5/10/20 uM with or without tamoxifen for 5 days and cell viability and mRNA expression of P-gp and BCRP were analyzed on day 5. As positive control, verapamil 50 uM was used as P-gp inhibitor, ritonavir 15 yM and nelfinavir 15 yM were used as BCRP inhibitor. Result:MCF-7(T) cells sensitivity to tamoxifen has decreased with 11,8 times reduction in sensitivity towards tamoxifen, the increased of cell viability, and mRNA expression of P-gp and BCRP mRNA increased 10,82 and 4,04 fold respectively in MCF-7(T) cells. Then after administration of curcumin with or without tamoxifenfor5 days then the cell viability and the mRNA expression of P-gp mRNA and BCRP decreased. Conclusion:Curcumin iIncreased the sensitivity of MCF-7(T) to tamoxifen, characterized by the decreased of cell viability and mRNA expression of P-gp and BCRP. But the administration of combination curcumin with tamoxifen was more potent than curcumin alone. The iIncreased sensitivity was estimated at least in part through the inhibition of P-gp and BCRP MRNA expression by curcumin. Background:The decreased of sensitivity or resistance to tamoxifen occured after long-term treatment in breast cancer. One of the major factor in tamoxifen resistance is over expression of efflux transporter P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP). Curcumin has known as inhibitor of P-gp and BCRP. The addition of curcumin to the tamoxifen resistant cells is expected to increase the sensitivity of breast cancer cells to tamoxifen. Method:MCF-7 breast cancer cell linewas induced with tamoxifen 1 uM for 10 passage (MCF-7(T)), then cell viability and mRNA expression of P-gp and BCRP were analyzed. To the MCF- 7(T) cells, curcumin was given at of 5/10/20 uM with or without tamoxifen for 5 days and cell viability and mRNA expression of P-gp and BCRP were analyzed on day 5. As positive control, verapamil 50 uM was used as P-gp inhibitor, ritonavir 15 yM and nelfinavir 15 yM were used as BCRP inhibitor. Result:MCF-7(T) cells sensitivity to tamoxifen has decreased with 11,8 times reduction in sensitivity towards tamoxifen, the increased of cell viability, and mRNA expression of P-gp and BCRP mRNA increased 10,82 and 4,04 fold respectively in MCF-7(T) cells. Then after administration of curcumin with or without tamoxifenfor5 days then the cell viability and the mRNA expression of P-gp mRNA and BCRP decreased. Conclusion:Curcumin iIncreased the sensitivity of MCF-7(T) to tamoxifen, characterized by the decreased of cell viability and mRNA expression of P-gp and BCRP. But the administration of combination curcumin with tamoxifen was more potent than curcumin alone. The iIncreased sensitivity was estimated at least in part through the inhibition of P-gp and BCRP MRNA expression by curcumin.
Co-Authors -, Paramita Achmad, Fariz Adam Iskandarmudasyah Adhayati, Baety Adisti Dwijayanti Agnes Frethernety Agus D. Susanto Ahmad Aulia Ali Sodikin Amanah Amanah Anang Endaryanto Andita Fitri Mutiara Rizki, Andita Fitri Mutiara Angelica Riadi Alim Saputro Angelina Riadi Alim Saputro Anom Bowo Laksono Anton Bahtiar Anton Bahtiar Ari Estuningtyas, Ari Aria Kekalih Arini Setiawati Arini Setiawati Arleni Arleni Aurelia Demtari Tuah Ayu Suraduhita Azmi, Wihda Aisarul Barasila, Atikah Chalida Budi Setiabudiawan Budi Wiweko Clara Riski Amanda Deni Rahmat Dewi, Yulia Ratna Didi Haryadi Didi Haryadi Dina Muktiarti, Dina Elisna Syahruddin Elly Yanah Arwanih Elvira Yunita Erlina Santoso Erni H. Purwaningsih Eziefule, Oluebube Magnificient Fajri, Purnama Fasha, Iqbal Franciscus D. Suyatna Frans D. Suyatna Gayatri, Anggi Gigih Andy Putra, Achmad Ginting, Tribowo T. Halim, Kelvin Handayani, Nur Hayati Dwi HANS-JOACHIM FREISLEBEN I MADE ARTIKA Indah D Dewijanti Indah D Dewijanti, Indah D Irandi Putra Pratomo Jeanne Adiwinata Pawitan Johannes Hudyono Kania, Theresia Kato, Mitsuyasu Ketut Dewi Kumara Wati Khariri Khariri Layal, Kamalia Made Susiyanti Madoka Takeuchi Manggiasih Dwiayu Larasati Masahiro Takeuchi Mega Putri Utami Mila Maidarti Mizuno, Seiya Muhaimin Muhaimin Mulyadi Djer Nabillah, Deya Adiby Nafrialdi Nafrialdi Nasrun, Martina WS. Ni Made Dwi Sandhiutami Nihayah, Silviatun Nining Handayani Novi S. Hardiany Novita, Rachma Nur Hayati Dwi Handayani Pandelaki, Jacub Paramita - Paramita - Paramita, Paramita Perdhana, Ika S. Puspita E. Wuyung Puspita Eka Wuyung Puspita Eka Wuyung Puteri, Meidi U. Putrihamidah, Diyanthie Aulia Rachma Novita Rachma Novita Rachman, Andika Radiana Dhewayani Antarianto Raymond R. Tjandrawinata Rebecca Noerjani Angka Resda Akhra Syahrani Rianto Setiabudy Rianyta Rianyta Rika Sari Dewi Safitri, Yolanda Sake Juli Martina SEKAR ARUMSARI, SEKAR Septelia I. Wanandi SEPTELIA INAWATI WANANDI Septelia Inawati Wanandi Setiabudy, Rahajuningsih D Shafilla Yunilma Andriany Sianipar, Erlia Anggrainy Sianipar, Erlia Anggrainy Siregar, Josephine Elizabeth Siste, Kristiana Sri W.A. Jusman Sumadiono Sumadiono Suratih, Ni Made Desy Susanto, Eka Syahrani, Resda A. Syarifah Dewi Tarigan, Immanuel N. Tri Yuliani Tri Yuliani, Tri Trishna, Alya R. Ungke Antonjaya Utami, Diah S. Vivian Soetikno Vivian Soetikno Vivian Soetikno Wahyunia Likhayati Septiana Wardhani, Bantari W.K. Watanabe, Yukihide Watanabe, Yukihide Wawaimuli Arozal Widyawati, Syska Yolanda Safitri Yolanda Safitri Zakiudin Munasir