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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Veteriner Jurnal Perikanan Kelautan Jurnal Natur Indonesia Journal of Aquaculture Management and Technology Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan SITEKIN: Jurnal Sains, Teknologi dan Industri EDULITE: Journal of English Education, Literature and Culture Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Creative Research Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Aquaculture and Fish Health Jurnal Kridatama Sains dan Teknologi Jurnal Magister Pendidikan Matematika (JUMADIKA) Jurnal Abdi Insani Jurnal Ilmiah MEA (Manajemen, Ekonomi, dan Akuntansi) Jurnal Kedokteran Hewan Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Terapis Gigi dan Mulut Jurnal Karya Generasi Sehat Journal of Fisheries & Marine JPEMAS: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmu Kesehatan
Sri Nuryati
Bogor Agricultural University
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Journal : Indonesian Aquaculture Journal

 1 
VIRULENCE GENE PROFILING AND PATHOGENICITY OF Streptococcus agalactiae ISOLATED FROM TILAPIA, Oreochromis niloticus FARMS IN INDONESIA Sukenda Sukenda; Achmad Suhermanto; Muhammad Zairin Jr.; Angela Mariana Lusiastuti; Sri Nuryati; Dendi Hidayatullah
Indonesian Aquaculture Journal Vol 16, No 2 (2021): (December, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.2.2021.119-125

Abstract

Streptococcosis caused by Streptococcus agalactiae has become a major disease problem in tilapia culture in Indonesia. This study aimed to detect virulence genes of S. agalactiae during streptococcosis disease outbreaks in several tilapia farms in Indonesia and evaluate the correlation between biotype and virulence genes to bacterial virulence. The presence of virulence genes was determined in 10 strains of S. agalactiae isolated from farm-raised tilapia. Polymerase chain reaction (PCR) protocol was used to determine genes for cylE, hylB, lmb, bib A, PI-2b, fbs A, fbs B, gap, PI-1, and cfb in the template DNA. Pathogenicity test was carried out by intraperitoneal injection of 24 hour-cultured S. agalactiae to tilapia with 108 CFU/fish. Four isolates have seven of virulence genes (cylE, hylB, bibA, PI-2b, fbs A, fbs B, and gap genes), three isolates have six virulence genes (hylB, bib A, fbs A, fbs B, gap, cfb genes), one isolate has four virulence gene (hyl B, bib A, fbs, and cfb genes), and one isolate has one virulence gene (PI-2b gene). None of the isolates has lmb or PI-1 genes. Bacteria with more virulence genes showed higher pathogenicity post injection. Mortality of tilapia injected with b-hemolytic bacteria was 100% within the period of 14-19 hours, while non-hemolytic bacteria was 53.3%-86.6% on 14 days post-injection. Pathological changes associated with the infection by either isolate included melanosis, slow response, anorexia, ocular opacity, gasping, erratic, C-shape, and whirling. It can be concluded that S. agalactiae with more virulence genes show a higher level of pathogenicity. The presence of a virulent gene has the potential to be used as a basis for selecting candidate isolates and designing vaccine compositions as an effort to prevent streptococcosis infection in tilapia in Indonesia.
IMMUNE RELATED GENES EXPRESSION ANALYSIS IN KOI FISH AFTER VACCINATED WITH KOI HERPES VIRUS DNA VACCINES Sri Nuryati; Fauzan Wahib Alsani; Hasan Nasrullah; Odang Carman; Yuni Puji Hastuti; Eni Kusrini; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 15, No 1 (2020): (June, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.1.2020.25-32

Abstract

Vaccination is a practical step in preventing diseases caused by koi herpes virus (KHV) in koi fish (Cyprinus carpio haematopterus). We have developed two DNA vaccines for KHV named as GP-25 and GP-11 from two local isolates coded as ORF25 and ORF81, respectively. Although both vaccines have been reported to increase survival rates, the evaluation of koi fish immune responses at the molecular level has not been done post-vaccinations. The aim of this research was to determine the effects of koi herpesvirus DNA vaccine on the immune-modulation of koi fish at mRNA level. This recent research used the best vaccine doses of both vaccines determined from our previous study: 7.5 and 12.5 µg per 100 g fish of GP-11, and 12.5 µg per 100 g fish of GP-25. The immune gene expression was analyzed using the RT-qPCR method from the fish liver at 0, 1, 7, 14, and 28 days post-vaccination (dpv). The results showed that, in the vaccinated fish, the immune genes viz. tumor necrosis factor a (TNFa), interleukin-1b (IL1b), interferon-g (IFNg), Mx1, immunoglobulin Mu chain (IgM), and major histocompatibility complex (MHC) class I and class II were induced to significant extents. The higher dose vaccination using the GP-11 vaccine showed higher immune gene expression than that of the lower dose. Furthermore, the GP-25 vaccine had induced lower immune responses than the GP-11 vaccine when using the same dose of vaccination, but relatively the same when the half-dose of GP-11 vaccine was used. In conclusion, the GP-11 and GP-25 vaccine provided the immune-modulatory effects on the koi fish immune response after vaccination.
EFFICACY OF GP-11 KHV DNA VACCINE IN Cyprinus carpio koi Sekar Ayu Chairunnisa; Sri Nuryati; Alimuddin Alimuddin; Sri Murtini; Ayi Santika; Dwi Hany Yanti
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (54.329 KB) | DOI: 10.15578/iaj.11.1.2016.31-39

Abstract

Koi herpesvirus (KHV) is one of the major pathogen for koi and common carp which cause high mortality and economic losses for the farmer. The purpose of this study was to determine the efficacy of glycoprotein-11 (GP-11) KHV DNA vaccine and compared to GP-25 KHV DNA vaccine. The vaccine in the form of naked DNA plasmidwas delivered by intramuscularly injection to the 3-month-old koi. The fish were divided into six groups, i.e. unvaccinated group (negative control C- and  positive control C+), and vaccinated group (2.5 μg/100 μl of GP-11 (group 1), 7.5 μg/100 μl of GP-11 (group 2), 12.5 μg/100 μl of GP-11 (group 3), and 12.5 μg/100 μl of GP-25 (group 4)). At day 42 post vaccination, all fish of each groups were challenged by injecting KHV titre 10-3 FID50. Number of dead fish was counted everyday after the challenge until 30 days. The results showed that vaccinated fish were had survival rate of 83.33-93.33% (group 2, 3 and 4). It’s show that GP-11 KHV DNA vaccine has high efficacy. As conclusion, GP-11 DNA vaccine could be an alternative DNA vaccine for preventing KHV infection.
EVALUATION OF RESISTANCE AND GENE EXPRESSION OF BARRAMUNDI, Lates calcarifer POST-INFECTION OF NERVOUS NECROSIS VIRUS Fitriyah Husnul Khotimah; Alimuddin Alimuddin; Dinar Tri Soelistyowati; Sri Nuryati; Ketut Sugama; Ahmad Muzaki; Indah Mastuti; Sari Budi Moria Sembiring; Ketut Mahardika; Harton Arfah; Haryanti Haryanti
Indonesian Aquaculture Journal Vol 17, No 2 (2022): (December, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.2.2022.97-106

Abstract

The most common problem in barramundi Lates calcarifer seedling production is the high mortality (> 90%) caused by nervous necrosis virus (NNV) infection. This research aims to evaluate the resistance and gene expression of barramundi challenged by NNV. Two populations were used in this study, i.e., Australian, and Situbondo-originated barramundi populations. The immune-related gene expression levels in the liver, head of kidney, and spleen were observed at 48 and 96 hours after post-infection (hpi). Barramundi’s survival and blood parameters were evaluated post-NNV infection. The results showed that the highest survival was revealed in Situbondo’s barramundi (42.0±4.47%) compared to Australian barramundi (20.0±7.07%) and no mortality was observed in the control without NNV infection. The higher survival rate in barramundi from Situbondo was in line with the blood profile. The number of red blood cell from Situbondo barramundi post-NNV infection (ST) at 96 hpi was higher (P<0.05) than Australian barramundi post-NNV infection (AT). The number of white blood cell of ST at 48 hpi was higher (P<0.05) than AT, but started to decrease at 96 hpi in ST barramundi. The total white blood cell in AT barramundi increased from 48 to 192 hpi. TNFα and IL1-β gene expression levels were significantly higher in the liver, head kidney, and spleen of Situbondo compared to Australian barramundi at 48 hpi, while MHCIIα gene expression in Situbondo’s was significantly higher compared to Australian barramundi at 96 hpi. These results indicate the important roles of all the genes in the barramundi’s immune responses against viral infection. Based on the results of the research, Situbondo’s barramundi has the potential to be used as a candidate for generating broodstock of disease-resistant strain.
EFFECT OF DIETARY BANANA PSEUDO-STEM SIMPLICIA, COMMERCIAL VACCINE, AND THEIR COMBINATION ON THE GROWTH, HEALTH STATUS, AND IMMUNITY PERFORMANCE OF NILE TILAPIA AGAINST Aeromonas hydrophila Wahjuningrum, Dinamella; Ramadhina, Erina Tri; Nuryati, Sri; Rizkiyanti, Ita; Abdullah, Taufiq
Indonesian Aquaculture Journal Vol 20, No 1 (2025): (June, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.1.2025.11-22

Abstract

The sustainability of Nile tilapia production faces challenges from motile Aeromonas septicemia (MAS), caused by Aeromonas hydrophila. The use of antibiotics to control MAS has negative impacts on aquatic environments and consumer health. As alternatives, plant-based treatments and vaccination have been increasingly applied to replace antibiotics. This study aimed to evaluate the effects of banana pseudo-stem (BS), a commercial vaccine (CV), and their combination (BS+CV) on the growth, health status, and immune performance of Nile tilapia against A. hydrophila. A completely randomized design was used with five treatments: a negative control (C−), a positive control (C+), BS, CV, and BS+CV—each coated onto feed. Each treatment had three replications. Fish were reared in 36-L aquaria for 42 days to evaluate growth performance, followed by a challenge test with A. hydrophila on day 43. Survival was monitored for 14 days post-challenge. The highest growth performance was observed in the BS treatment compared to the other treatments. Meanwhile, survival rate (SR) and feed conversion ratio (FCR) did not show significant differences (P>0.05) among treatments. After the challenge, survival rates in the BS (96.67%), CV (73.33%), and BS+CV (76.67%) groups were significantly higher (P<0.05) than in the positive control group (50.00%). The BS group did not differ significantly (P>0.05) from the negative control group (100.00%). These findings indicate that BS, CV, and BS+CV enhance the immune response of Nile tilapia against A. hydrophila, with BS being the most effective in improving both growth and disease resistance.
Co-Authors , Alimuddin , Rahman, , , Rusli, , , Sofian, , , Yuliyanti . Kurdianto . Taukhid A. Santika A. Suwanto Adi Susanto, Riki Aditya, Tiya Widi Afif Abdurrahman Afiff , Usamah Agung Cahyo Setyawan Agus Oman Sudrajat Agussalim, Anita Mardiana Ahmad Beni Rouf Ahmad Firdaus, Ahmad Ahmad Muzaki Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin amperawati, metty Amperawati2, Metty Andi Tiara Eka Diana Puteri, Andi Tiara Eka Diana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Nugrahawati Anita Setyowati Srie Gunarti Ardana Kurniaji Asrido, Farhan Astu Rahman, Waljuni Ayi Santika Ayi Santika Ayu Dhita Juliadiningtyas, Ayu Dhita Bagus Rahmat Basuki Brite, Margie BUNGA NURWATI D. Meha D. Puspitaningtyas D. Wahjuningrum Dadi, Oswaldus Danan, Danan DEDI JUSADI Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah, Dendi Dian Febriani Dian Hardiantho Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Hany Yanti Dwi Hany Yanti Eddy Supriyono Eko Darma Elma yulius Elman Efzi, Agno Eni Kusrini Eni Kusrini Erni Susanti F.B.P. Sari F.H. Pasaribu Fachriyan Hasmi Pasaribu Fajar Prihesnanto, Fajar Fajrin, Anang Falah, Fazrul Faoziyatunnisa, Nurul Fateah, Nur Fauzan Wahib Alsani Firdausi, Amalia Putri Firmansyah, Arif Lukman Fitriyah Husnul Khotimah Goro Yoshizaki Gusti Ngurah Permana Habibah, Siti Sab'atul Habibah, Siti sabatul Hanggara, Yudha Harton Arfah Haryanti Haryanti Haryanti Haryanti Hasan Nasrullah Hidayah, Winda Alfina Ibnu Bangkit Bioshina Suryadi IDA RAHMAWATI Iis Widiani Ikhsan Khasani Indah Mastuti ISNAWATI Isnawati Isnawati Isni Rahmatika Sari Julie Ekasari K. Sumantadinata Ketut Mahardika Ketut Sugama Ketut Sugama Khotimah, Fitriyah Husnul Kisworo Utami, Naning Komar Sumantadinata Kukuh Nirmala L.S. Harti La Ode Muhammad Arsal Lili Sholihah Lilis Nurjanah M. Zairin Junior M.A. Suparman Maharani, Rizqya Mia Setiawati Mira Mawardi Muchtar, Muthahharah MUHAMMAD AGUS SUPRAYUDI Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mukni Mukni, Mukni Mulyani, Rahma Munawarah, Husnadatul MUNTI YUHANA N. Ashry N.A. Maswan Nababan, Yanti Inneke Nadia Aulia, Nadia Nasrullah, Hasan Ninik Paryati Nisa, Hilwatun Nugroho, Sigit Dani Nur Rahmawati Nurbariah Nurbariah Nurhasanah Nurina Pratiwi Nurly Faridah Nurly Faridah NURWATI, BUNGA Odang Carman P. Giri P. Hariyadi Priyoutomo, Nur Bambang Puteri, Rafilah Putri, Elva Khairina Qorie Astria R.D. Soejoedono Raharjo, Pamuji Gesang Rahma Mulyani Rahmadiniyah, Rahmadiniyah Rahman Rahman, Waljuni Astu Rahmi, Kurnia Anggraini Rahminiwati, Min Ramadhan, Afriani Ramadhani, Danika Athirah Ramadhina, Erina Tri Retno Damayanti Soejoedono Rizkiyanti, Ita S.H. Sholeh Said, Fahmi Sari Anggraeni, Sukma Sari Budi Moria Sembiring Sari Budi Moria Sembiring Sarjito - SATRIYAS ILYAS Sekar Ayu Chairunnisa Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Septia Armayani, Dilla Siti Khodijah Siti Murniasih Siti Salamah Siti Zubaidah Slamet Budi Prayitno Soko Nuswantoro sri murtini . Suhermanto, Achmad Sukenda . Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda, Sukenda, Suryani, Dessy Rizki Taufiq Abdullah, Taufiq Tiara Puspa Anjani Tira Silvianti Titin Kurniasih Trian Rizky Febriansyah Tuti Sumiati Tuti Sumiati Utami, Naning K. Utomo, Nurbambang Priyo Uttari Dewi Wahyuwardani , Sutiastuti Wasmen Manalu Wida Lesmanawati WIDANARNI WIDANARNI Y. Hadiroseyani Y. Kuswardani Yanti, Mauliza Yuni Puji Hastuti Yusril Pratama, Muhammad Zakki Zainun Zulhelmi, Arif