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Ira Rahmawati
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Journal : Jurnal Biosense

 1 
IDENTIFIKASI DIVERSITAS MIKROBA PADA PANGAN ROTI EXPIRED DAN NON-EXPIRED Ira Rahmawati; Labibah Fatihatu hanin; Muhimatul Ummami
JURNAL BIOSENSE Vol 8 No 1 (2025): Edisi Januari 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i1.4848

Abstract

This study aims to identify microbial biodiversity in bread that has not passed its expiration date (non-expired) and that has passed its expiration date (expired). The research methods include microbial isolation, colony morphology identification, Gram staining, biochemical tests (sugar, catalase, motility, and oxidative-fermentative tests), and diversity analysis using the Shannon-Wiener index. The results showed that microbes in expired bread tend to be more varied than non-expired bread, with the number of colonies increasing with the length of the expiration date. Gram staining showed the dominance of Gram-negative bacteria, although Gram-positive bacteria were also found. Biochemical test analysis revealed the dominant glucose fermentation ability in all colonies. Based on the diversity analysis, the highest level of microbial diversity was found in 8-day expired bread with an index of 0.67, while non-expired bread had an index of 0.56. These findings emphasize the importance of paying attention to the expiration date of bread to prevent microbial contamination that is potentially harmful to health.
Co-Authors 'Athiyah, Qoni’atul Abdulloh Faqih S. M. Ade Holis Ade Ratnasari Af'idatur Rosyidah Afdi Septiono, Eka Afidah, Nurul Ahmad Junaidi Alfarizi, Muhammad Alfia, Siti Mafudlotul Alfurqon, Achmad Syarofi Amartha, Desta Cahya Amerta, Midya Yuli Anggraini, Dina Dewi Anwar, Muhammad Saidun Ariani, Febrian Dwi Eki Putri Astuti, Dwi Hery Ateng Supriatna Azizah, Alfiani Nur Busriyanti Busriyanti Deditiani Tri Indrianti Della Nisa'ul Mufida Dewi Nishwatul Fithriyah Dewi, Fitria Dilaruri, Ade Dina Dewi Anggraini Dini Kurniawati Dzunnuroin, Naura Royyani Edi Rohaedi Eka Afdi Septiyono Elan, Elan Fauza, M. Ruskhan Fina Riyandita Masfurin Hafni Zukhrufina Hilwa Kamilatunnuha I Made Maduriana Iis Komariah Indah Siti Aisyah Inganah Istiyati Mahmudah Iswati Iswati Ita Fitriyah Julianingrum, Peni Perdani Julinigrum, Peni Perdani Juliningrum, Peni Perdani Kornalisa, Kornalisa Labibah Fatihatu Hanin Lantin Sulistiyorini, Lantin Lasmanah Latifah, Aurora Anastasya Lingling Marinda Palupi Lisabella, Stefanie Hapy Llopis , José Luis Luh Putu Ratna Sundari Luluk Nurul Aini Luthfi Hana Fadiah Makhrufi M.A.S. Ardianto Mareta, Lutvia Masripah Merina, Nuning Dwi Midya Yuli Amreta MUHAMMAD IRFAN HILMI Muhammad Mubiar Ramadana Muhammad Rafi Fauzan Muhimatul Ummami Nabilah Artha D.Z. Nadia Meirani Ni Made Rai Wisudariani Ni Putu Seniwati, Ni Putu Nourma Andini, Aisyah Novananda, Ajie Nunuy Nurjanah Nurhidayah, Ririn Nurul Afidah NURUL HIDAYAH Nurul Hidayah OPAH ROPIAH, OPAH Peni Perdani Juliningrum, Peni Perdani Perdani Juliningrum, Peni Perwiraningrum, Dhyani Ayu Rahmawati, Iis Rajabani, Alissa Zakiah Retty Isnendes Risa Dwita Hardianti, Risa Dwita Rizanti, Ayunda Puteri RUHALIAH Dr., RUHALIAH Saidah, Qori Ila Sani Saputra, Andika Ari Saputra, Erwin Rahayu Sari, Marlynda Happy Nurmalita Satya, Malinda Capri Nurul Setyowati, Lisus Sinta Rosalina Stephani Diah Pamelasari, Stephani Diah Sufyan Asasi, Muhammad Sulastyawati Sulastyawati Supono Suprianti, Lilik Susanti, Ika Adelia Tri Cahyanto Triyadi, Slamet Tulus Rega Wahyuni E Wibisono, Sentot Satrio Widiani, Esti Yulia Indrawati Zulkarnain Zulkarnain