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Journal : BIOVALENTIA: Biological Research Journal

THE ESSENTIAL CONTRIBUTION OF CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK FOR WILDLIFE GENETICS CONSERVATION Rustiati, Elly Lestari; Priyambodo, Priyambodo; Yulianti, Yanti; Srihanto, Eko Agus; Pratiwi, Dian Neli; Virnarenata, Elsa; Novianasari, Tika; Krismuniarti, Elisabeth Devi; Saswiyanti, Enny
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.173 KB) | DOI: 10.24233/BIOV.6.1.2020.173

Abstract

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.
IDENTIFICATION AND CHARACTERISATION OF COI GENE IN FEMALE SUMATRAN ELEPHANT (Elephas maximus sumatranus) IN ELEPHANT TRAINING CENTRE, WAY KAMBAS NATIONAL PARK Virnarenata, Elsa; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Pratiwi, Dian Neli
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (716.49 KB) | DOI: 10.24233/biov.7.1.2021.175

Abstract

Sumatran elephant is a subspecies of endemic Asian elephants on the island of Sumatra and is included in the Red List of the International Union for Conservation of Nature (IUCN) with critically endangered status. The building of the Elephant Training Centre (ETC) in Way Kambas National Park (WKNP) is one of the conservation efforts of Sumatran elephants. Small and closed population size lead to an increased risk of inbreeding that triggers reduction in genetic variation and viability and increases the risk of extinction. The phylogenetic pattern of Sumatran elephants in Indonesia has shown a low population genetic diversity. Genetic diversity information is indispensable to support the direction of decision making in Sumatran elephant conservation policy. The DNA isolation of Sumatran elephants in ETC, WKNP has performed as a first step to trace its genetic variation. The advanced step of DNA isolation is the use of cytochrome oxidase subunit I (COI) gene for identification of genetic characteristics in Sumatran elephants. The COI gene is one of the genes on the mitochondrial genome and in molecular studies it is used as a genetic marker to study genetic characteristics between species and individuals. Identification and characterisation are done by sequencing process and data analysis in the form of electroforegram using Molecular Evolution Genetics Analysis (MEGA) software version 6.0. to see the genetic diversity of the female Sumatran elephant population in ETC, WKNP. Based on the results of the analysis it is indicated that the genetic distance of 24 individual female Sumatran elephant from PLG, TNWK is 0.000 with a homology value of 100%, strengthened by the construction of phylogenetic tree. The absence of genetic distance indicates a close genetic relationship, so it can be concluded all individual female Sumatran elephants in the PLG, TNWK is derived from one population group.
PRIMER OPTIMIZATION AS GENETIC MARKER PRIMER BASED ON CYTOCHROME B GENE ON SUMATRAN ELEPHANT (Elephas maximus sumatranus) NON-INVASIVE SAMPLES Eko Agus Srihanto; Elly Lestari Rustiati; Priyambodo Priyambodo; Dian Neli Pratiwi; Alvin Wiwiet Susanto; Diah Esti Anggraini; Enny Saswiyanti
BIOVALENTIA: Biological Research Journal Vol. 8 No. 1 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.02 KB) | DOI: 10.24233/biov.8.1.2022.216

Abstract

Sumatran elephant is declared as critically endangered by IUCN since 2011. In supporting conservation efforts on sumatran elephant in its natural habitat, molecular data collection of their population needs to be done. Genetic source material is mostly obtained in its natural habitat is its dung/feces. Study on cytochrome B gene as genetic marker from sumatran elephant fecal samples can be done to get additional genetic data as a form of conservation effort. Primer optimization as genetic marker based on cytochrome B gene has been carried out as an early study on sumatran elephant genetics.  Primer optimization program based on cytochrome B genetic marker from sumatran elephant fecal samples was under Higher Education Applied Research funded by The Ministry of Research, Technology, and Higher Education. DNA was isolated from fecal samples of captive sumatran elephants in Elephant Training Center, Way Kambas National Park. Primer optimization was done via amplification by increasing certain temperature. Specificity test was done to ensure that the primer only recognized sumatran elephant DNA. The best result was obtained with the annealing temperature of 52oC
Co-Authors 'Aliyah, Siti Hamidatul A.Basyir Firdaus Adelia, Ersa Imelda Adi, Marcelus Adinda, Calesta Afandi, Aril Agus Setiawan AGUS SETIAWAN Agus Setiawan Agus Subagyo Ahyul Heni Al Amin, Muhammadi Ridho Alfandi, Aril Alimudin, Salih Alvin Wiwiet Susanto Alvin Wiwiet Susanto Angga Kurniawan Angga Pramudya Angga Pramudya Anggy, Fransiska Panasea Apriani, Vivin Apriliasari, Messy Aprio, Yogi Aril Afandi Ariyanto, Slamet Puji Aryani, Nina Yudha Ashari, Minanti Mayda Azmi, Sevira Nur Azzahra, Laila Salwa Bayu , Raden Fadli Bondan Pergola Cicilia, Okferina Cindy Yoeland Violita Cindy Yoeland Violita Daely, Sofia Vao Afni Diah Esti Anggraini Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dina Farida Utami Dina Farida Utami Dwi Asmi Dwi Astuti, Putri Dwi Ayu Febriyani Dwitama , Harry Putra Edi Santoso Edi Santoso Edo Firnanda Edo Firnanda, Edo Eka Sulpin Ariyanti Eka Sulpin Ariyanti, Eka Eko Agus Prihanto Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto, Eko Agus Elfita Nova Yunior Elsa Virnarenata Emantis Rosa Enny Saswiyanti Enny Saswiyanti Erdi Suroso Fahrezi, Aditya Febriansyah, Muhammad Febriyani, Dwi Ayu Feni Yulinda Firwantoni Firwantoni Firwantoni, Firwantoni Fitrisyah, Asyifa Zahara Gina Dania Pratami, Gina Dania Haryanto, Sugeng Prayitno Heni, Ahyul Hidayatulloh I Gede Swibawa I Wayan Tony Candra Ibransyah, Rasyid Ifan Wahyudi Ignatius Suharto Imtitsal, Aulia Indah Ayu Lestari Indah Ayu Lestari, Indah Ayu Intan Diani Fardinatri Iqbal Amiruddin Ihsanu Iqbal Amiruddin Ihsanu Irawati, Anie Rose IRFAN NURARIFIN, IRFAN Irwan Nasution Jani Master Jani Master, Jani Kasturi Khalil, Abdul Rouf Amarulloh Khumaidi, Fauzan Krismuniarti, Elisabeth Devi Kurniasih, Vidyanti Kusuma Handayani, Kusuma Kusuma, Andriyani Wijaya Lestari, Septi Wahyu Maharani, Annisa Lidya Marcelus Adi Minanti Mayda Ashari Muhammad Febriansyah Muhammad Rohiyan Muhammad Rohiyan Natasya Thesalonika Neli Pratiwi, Dian Novianasari, Tika Nuning Nurcahyani Panasea Anggy, Fransiska Parabi, M. Iqbal Pawaka, Arrahmaan Syah Permatasari, Nindy Pratama, M. Idris Afta Pratiwi, Dian Neli Pratiwi, Ratih - Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Puspita Widiarini, Dewi Raden Fadly Bayu Dwiyoga Raden Mohamad Herdian Bhakti Ramadan, Viki Ramadhan, Viki Ramadhanti, Saskya Adrila Rhamadaningtyas, Nabila Aulia Rodliyah, Aini Robby Romaya Wulan Suciningtyas Rosa, Emantis Sa'uddah, Lousanja D. Safitri, Anggi Salih Alimudin Sandora, Desta Sandra, Shifa Saputra, Rama Arsalta Bara Saputri, Nur Ayu Saskya Ramadhanti Saswiyanti, Enny Sendora, Desta Septi Wahyu Lestari Septiana, Ana Sevira Nur Azmi Sevira Nur Azmi Shifa Sandra Sidik, Mahfud Sinurat, Lidya Septaria Soegiharto, Yolande Cathleya Sri Retnaning Rahayu, Sri Retnaning Suhada Suhada, Suhada Suharto, Ignatius Sumaryo Sunandar Sunandar Sunandar Sunandar Supriyadi, Tatang Suratman Umar Susandi, Waryoko Susanti, Iproh Susanto, Alvin Wiwiet Susilawati Suwarno Syarif Hidayat Amrullah Teddy TRIANDIZA Thesalonika, Natasya Tugiyono Tugiyono Tugiyono Tugiyono Tugiyono Utoyo, Laji Vega, Cindy Ameliya Vidya Kurniasih Vidyanti Kurniasih Virnarenata, Elsa Wahyono, Agus Waryoko Susandi WINARNO Winarno Winarno Yani, Ani Andri Yanti Yulianti Yogi Aprio Yuliana Andriyani Yuni Tinasari Yunior, Elfita Nova Zacky Zeinel Ibad Muchlas Zamroni