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All Journal Biospecies Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biotika: Jurnal Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Penelitian Sains Jurnal Biologi Tropis J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Biogenerasi BIO-EDU: Jurnal Pendidikan Biologi IJOEP : International Journal of Ecophysiology Biofarm : Jurnal Ilmiah Pertanian Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun BIOVALENTIA: Biological Research Journal Jurnal Pepadun Jurnal Teknologi Lingkungan Lahan Basah Seandanan: Jurnal Pengabdian pada Masyarakat Jurnal Sylva Lestari Kenanga : Journal of Biological sciences and Applied Biology Jurnal Mitrawarga Organisms: Journal of Biosciences AMMA : Jurnal Pengabdian Masyarakat Journal Of Medicine And Health Sciences (Medisci) Journal Of Agriculture Sciences (Agrosci) Journal of Community Service (Abdisci) Journal Of Engineering Sciences (Improsci)
Elly Lestari Rustiati
Universitas Lampung
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Daya Tampung Beban Pencemaran Sungai Way Umpu, Provinsi Lampung Sinurat, Lidya Septaria; Tugiyono, Tugiyono; Umar, Suratman; Rustiati, Elly Lestari
Jurnal Teknologi Lingkungan Lahan Basah Vol 12, No 1 (2024): Januari 2024
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jtllb.v12i1.73771

Abstract

Sungai Way Umpu berada di wilayah Kabupaten Way Kanan, Provinsi Lampung. Sungai ini memiliki lahan pada daerah aliran sungai yang dimanfaatkan oleh penduduk sekitar. Limbah buangan menuju sungai dihasilkan oleh lahan yang dapat menyebabkan peningkatan beban pencemaran melebihi kemampuan daya tampung sungai. Penelitian ini bertujuan untuk mengukur daya tampung beban pencemaran dan mengetahui parameter kualitas air yang melebihi baku mutu di Sungai Way Umpu. Penelitian dilakukan pada empat stasiun penelitian. Hasil pengukuran kualitas air dibandingkan dengan baku mutu kelas III sesuai Peraturan Daerah Provinsi Lampung no 11 tahun 2012. Metode neraca massa digunakan untuk menganalisis daya tampung pada titik tampung stasiun 4 (ST-4). Hasil penelitian menunjukkan kualitas air pada ST-3 untuk parameter BOD telah melebihi baku mutu. Sedangkan hasil analisis daya tampung pada titik tampung (ST-4) untuk parameter (nitrat, DO, BOD, dan total coliform) masih dalam standar kisaran baku mutu Kelas III, sehingga Sungai Way Umpu masih memiliki kemampuan menampung beban pencemaran dan dapat digunakan sesuai peruntukannya.
Keragaman Genetik Kopi Robusta Berdasarkan Karakter Fenotipe Batang di Perkebunan Kopi Rakyat Bogorejo, Lampung: Genetic Diversity of Robusta Coffee Based on Stem Phenotypic Characters in Bogorejo Coffee Plantation, Lampung Thesalonika, Natasya; Sandra, Shifa; Priyambodo, Priyambodo; Rustiati, Elly Lestari; Winarno, Winarno; Lestari, Septi Wahyu; Ashari, Minanti Mayda; Andriyani, Yuliana; Maharani, Annisa Lidya; Afandi, Aril; Febriansyah, Muhammad; Pratiwi, Dian Neli; Permatasari, Nindy; Suhada, Suhada
Biofarm : Jurnal Ilmiah Pertanian Vol. 21 No. 1 (2025): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v21i1.5937

Abstract

Pengembangan bibit unggul pada tanaman kopi robusta dapat dilakukan dengan pengamatan pada karakter batang. Hal ini penting mengingat kopui robusta menjadi salah satu penyumbang devisi dan sumber komoditas uatam di Indonesia, khususnya Lampung. Penelitian dilaksanakan untuk mengamati keragaman genetik tanaman kopi robusta pada Perkebunan Kopi Rakyat di Desa Bogorejo, Kecamatan Gedong Tataan, Kabupaten Pesawaran, Provinsi Lampung berdasarkan karakteristik fenotipe batang. Penelitian dilakukan dengan purposive sampling pada 30 individu. Pengamatan kualitatif dilakukan pada lima karakter batang, sedangkan pengukuran karakter kuantitatif dilakukan pada 11 karakter batang. Hasil menunjukkan bahwa terdapat 11 individu yang mempunyai sambungan batang bawah-batang atas, dengan seluruh individu tanaman kopi robusta mempunyai warna dan permukaan batang yang seragam, baik batang bawah dan batang atas. Di sisi lain, pengamatan terhadap karakter kuantitatif menunjukkan ragam yang lebih tinggi sehingga nampak adanya pengaruh genetik dan lingkungan terhadap karakter fenotipe batang tanaman kopi. Adapun rerata karakter kuantitatif individu pada populasi tersebut adalah tinggi tanaman total 124,29 cm, tinggi batang bawah 98,30 cm, lingkar batang bawah 15,05 cm, diameter batang bawah 47,25 cm, tinggi batang atas 70,88 cm, lingkar batang atas 14,78 cm, diameter batang atas 46,41 cm, jumlah cabang ortrotop pada batang utama 1,60 buah, jumlah jabang plagiotrop pada tabang utama 8 buah, tinggi cabang ortotrop pertama pada batang utama 39,43 cm, dan tinggi cabang plagiotrop pertama pada batang utama 114,30 cm.
Traffics and wildlife: A preliminary study on road-kill Rustiati, Elly Lestari
Biospecies Vol. 2 No. 2 (2009): Juni 2009
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v2i2.252

Abstract

This paper presents the preliminary finding on road kill survey by direct observations onthe high ways. The road-kills recorded of small wildlife, including medium size-mammal (2.50%, n =1), birds (5.00%, n = 2) and small mammals (92.50%, n = 37). The small mammals include the mostcommon mammals in the areas, squirrels, raccoons, skunks and woodchuck. Of mammals, squirrels(35.00%) were the highest recorded, followed by woodchucks (25.00%), mice/shrew (17.50%),raccoons (10.00%), skunk (5.00%) and domestic cat (2.50%) respectively. It seems road-kill wascaused by conflict between the wildlife natural habitat as well as its ecological behavior and roaddevelopment.
Dilema Ekologis Masyarakat Setempat dan Babi Hutan di Perbatasan Taman Nasional Way Kambas Wahyudi, Ifan; Rustiati, Elly Lestari
Biospecies Vol. 2 No. 1 (2009): Januari 2009
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v2i1.258

Abstract

Makalah ini membahas tentang dilema gangguan babi hutan di perbatasan TamanNasional Way Kambas. Penelitian ini bekerjasama dengan Balai Taman Nasional Way Kambas,dilakukan pada bulan Maret – April 2005 di desa Labuhan Ratu VI dan VII. Metode yang digunakanadalah survei angket dan wawancara. Untuk mempelajari tipe habitat secara umum digunakanmetode rapid assessment. Pengetahuan tentang keberadaan babi hutan oleh masyarakat setempatcukup tinggi terutama berdasarkan perjumpaan langsung dan bekas jejaknya. Babi hutan biasanyadijumpai dalam kelompok pada sore dan malam hari di perkebunan singkong dengan vegetasisemak. Gangguan kerusakan hanya ditemukan pada perkebunan singkong. Pandangan masyarakat terhadap babi hutan sebagai hama menunjukkan adanya keterkaitan terhadap keberlangsungankehidupan masyarakat setempat dan ancaman populasi babi hutan
Local People And Primates In The Way Kambas National Park : Going Towards Co-Existence Or Conflict? TRIANDIZA, Teddy; Rustiati, Elly Lestari
Biospecies Vol. 2 No. 1 (2009): Januari 2009
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v2i1.263

Abstract

Kajian bekerja sama dengan Taman Nasional Way Kambas dilakukan untuk mengamatiperjumpaan dan gangguan primata di desa yang berdekatan dengan perbatasan taman nasional,Labuhan Ratu VI dan Labuhan Ratu VII. Survei lapangan dan penyebaran angket digunakan dalampengumpulan data. Perjumpaan dengan primata cukup tinggi. Di antara keberadaan empat primate,monyet ekor panjang dan beruk paling sering dijumpai secara langsung. Dua titik perjumpaanberdekatan dengan perkebunan singkong. Primata belum dianggap sebagai hama pengganggukarena adanya ancaman yang lebih besar dari gajah Sumatera dan babi hutan. Namun demikianpenemuan ini harus dijadikan pertimbangan untuk upaya konservasi yang berkaitan denganmasyarakat setempat dan primata di Taman Nasional Way Kambas.
Oral Swab-Based DNA Extraction Of Cynopterus Brachyotis: An Initial Step For Species Confirmation Rhamadaningtyas, Nabila Aulia; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Saswiyanti, Enny; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet
Jurnal Agrosci Vol 3 No 3 (2026): Vol 3 No 3 January 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i3.1025

Abstract

Background. Identifying bat species based on morphological characteristics often faces challenges due to character overlap among species, especially within cryptic species groups. Cynopterus brachyotis is one of the fruit bats with high genetic diversity but relatively uniform morphology. Aims. Therefore, a molecular approach is needed to support accurate species confirmation. Under the HETI Research Grant of Innovation and Collaboration Batch 3- II Year 2025, this study aims to obtain genomic DNA from C. brachyotis bats caught at the Lampung Disease Investigation Center as the initial step of molecular species confirmation. Methods. Samples were obtained by taking oral swabs on bat individuals caught using mist nets. DNA extraction is performed using silica membrane-based methods with commercial kits. Quantitative evaluation of DNA was performed using a Qubit Fluorometer, while qualitative evaluation was performed by agarose gel electrophoresis. Conclusion. The results showed that the DNA concentration was in the low range (0.1–0.7 ng/μL), with a DNA band appearing very thin on electrophoresis. Implementation. Nevertheless, the quantification results confirm that DNA was successfully extracted and remains suitable for advanced molecular analysis, with optimization at the DNA amplification stage.
DNA Extraction of Cynopterus brachyotis of Labuhan Ratu VII Based on Column Method in Supporting its Molecular Species Confirmation Maharani, Annisa Lidya; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Saswiyanti, Enny; Neli Pratiwi, Dian; Susanto, Alvin Wiwiet
Jurnal Agrosci Vol 3 No 1 (2025): Vol 3 No 1 September 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i1.1043

Abstract

Background. Bats (Chiroptera) are true flying mammals and nocturnal. Ecologically, bats play important roles as pollinators, seed dispersers, guano producers, biocontrol, and hosts a range of viruses. Cynopterus brachyotis belongs to fruit-eating bats and is considered morphologically cryptic with C. sphinx. Aims. To support morphological identification, molecular species confirmation needed to be conducted. DNA extraction, as an initial step, plays a crucial role in molecular analysis. Methods. The quality and quantity of extracted DNA determine the success rate of sequencing for species confirmation in bats. In this study, oropharyngeal samples were used. Result. The research procedures included bat capture and DNA oropharyngeal swab sample collection, sample preparation, DNA extraction using a silica column-based commercial kit, DNA concentration measurement using a Qubit assay, and electrophoresis. Conclusion. The Qubit assay showed DNA concentrations of >1 ng/µL, while electrophoresis did not reveal bright DNA bands.
Learning Bats Handling For Oral Swab Sampling Azmi, Sevira Nur; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Saswiyanti, Enny; Srihanto, Eko Agus; Pratiwi, Dian Neli; Susandi, Waryoko
Jurnal Medisci Vol 3 No 1 (2025): Vol 3 No 1 August 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/medisci.v3i1.1014

Abstract

Background. The only flying mammals, bats, play an essential role in their natural habitat. Based on the type of food, bats can be divided into two groups: fruit-eating bats and insect-eating bats. Bats rank second-highest in species diversity, with 1,439 worldwide and 239 in Indonesia. Known as reservoirs of viruses, including coronaviruses. Aims. Bats are suspected to be related to COVID-19. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) University of Lampung 2024-2025, and in collaboration with the Lampung Disease Investigation Centre, learning the procedures for handling bats properly so as not to physically harm bats in oral swab sampling and species recognition in Braja Harjosari, directly next to Way Kambas National Park was done. Methods. The life-trapping technique uses a mist net. Bat handling for taking oral swab was carried out using the pinch grip method, which is holding both arms of the bat backwards utilizing the thumb and middle finger with the bat positioned facing upwards. Conclusion. The bat's mouth is then blown open and a cotton swab is gently inserted into the bat's mouth. Afterwards, the bat is rested, given water drops, and released into nature. Oral swab samples from 10 individual bats: frugivorous bats, Cynopterus brachyotis (n = 8), Cynopterus horsfieldii (n = 1), and insectivorous bat, Scotophilus kuhlii (n = 1).
Analysis Of Cadmium (Cd) And Lead (Pb) Levels In Water Samples By Atomic Absorption Spectrophotometry (AAS) At The Lampung Health Laboratory Center Adinda, Calesta; Rustiati, Elly Lestari; Susanti, Iproh; Cicilia, Okferina
Jurnal Improsci Vol 3 No 4 (2026): Vol 3 No 4 February 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/improsci.v3i4.1053

Abstract

Background. For sanitary hygiene, water is used for maintaining personal hygiene, such as bathing and brushing, as well as for food washing, dishwashing, and laundry in daily life, so its quality differs from that of drinking water. This study focuses on heavy metal pollution, particularly lead (Pb) and cadmium (Cd), which are known toxic substances harmful to living organisms. Aims. Its purpose is to determine the levels of cadmium and lead in water samples by atomic absorption spectrophotometry at the Lampung Health Laboratory Center, conducted in December 2024-January 2025. T Methods. The method refers to SNI No. 6989.84 of 2019 for 78 samples of sanitary hygiene water by Atomic Absorption Spectrophotometry (SSA). Cadmium (Cd) analysis of 37 samples: 21 were positive for Cd, but none exceeded the threshold limit value of 0.003 mg/L. Conclusion. Meanwhile, the results of the lead (Pb) analysis showed that 1 of 37 samples did not exceed the threshold limit value of 0.01 mg/L, as stipulated in the Indonesian Minister of Health Regulation No. 2 of 2023.
Co-Authors 'Aliyah, Siti Hamidatul A.Basyir Firdaus Adelia, Ersa Imelda Adi, Marcelus Adinda, Calesta Afandi, Aril Agus Setiawan AGUS SETIAWAN Agus Setiawan Agus Subagyo Ahyul Heni Al Amin, Muhammadi Ridho Alfandi, Aril Alimudin, Salih Alvin Wiwiet Susanto Alvin Wiwiet Susanto Angga Kurniawan Angga Pramudya Angga Pramudya Anggy, Fransiska Panasea Apriani, Vivin Apriliasari, Messy Aprio, Yogi Aril Afandi Ariyanto, Slamet Puji Aryani, Nina Yudha Ashari, Minanti Mayda Azmi, Sevira Nur Azzahra, Laila Salwa Bayu , Raden Fadli Bondan Pergola Cicilia, Okferina Cindy Yoeland Violita Cindy Yoeland Violita Daely, Sofia Vao Afni Diah Esti Anggraini Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dina Farida Utami Dina Farida Utami Dwi Asmi Dwi Astuti, Putri Dwi Ayu Febriyani Dwitama , Harry Putra Edi Santoso Edi Santoso Edo Firnanda Edo Firnanda, Edo Eka Sulpin Ariyanti Eka Sulpin Ariyanti, Eka Eko Agus Prihanto Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto, Eko Agus Elfita Nova Yunior Elsa Virnarenata Emantis Rosa Enny Saswiyanti Enny Saswiyanti Erdi Suroso Fahrezi, Aditya Febriansyah, Muhammad Febriyani, Dwi Ayu Feni Yulinda Firwantoni Firwantoni Firwantoni, Firwantoni Fitrisyah, Asyifa Zahara Gina Dania Pratami, Gina Dania Haryanto, Sugeng Prayitno Heni, Ahyul Hidayatulloh I Gede Swibawa I Wayan Tony Candra Ibransyah, Rasyid Ifan Wahyudi Ignatius Suharto Imtitsal, Aulia Indah Ayu Lestari Indah Ayu Lestari, Indah Ayu Intan Diani Fardinatri Iqbal Amiruddin Ihsanu Iqbal Amiruddin Ihsanu Irawati, Anie Rose IRFAN NURARIFIN, IRFAN Irwan Nasution Jani Master Jani Master, Jani Kasturi Khalil, Abdul Rouf Amarulloh Khumaidi, Fauzan Krismuniarti, Elisabeth Devi Kurniasih, Vidyanti Kusuma Handayani, Kusuma Kusuma, Andriyani Wijaya Lestari, Septi Wahyu Maharani, Annisa Lidya Marcelus Adi Minanti Mayda Ashari Muhammad Febriansyah Muhammad Rohiyan Muhammad Rohiyan Natasya Thesalonika Neli Pratiwi, Dian Novianasari, Tika Nuning Nurcahyani Panasea Anggy, Fransiska Parabi, M. Iqbal Pawaka, Arrahmaan Syah Permatasari, Nindy Pratama, M. Idris Afta Pratiwi, Dian Neli Pratiwi, Ratih - Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Puspita Widiarini, Dewi Raden Fadly Bayu Dwiyoga Raden Mohamad Herdian Bhakti Ramadan, Viki Ramadhan, Viki Ramadhanti, Saskya Adrila Rhamadaningtyas, Nabila Aulia Rodliyah, Aini Robby Romaya Wulan Suciningtyas Rosa, Emantis Sa'uddah, Lousanja D. Safitri, Anggi Salih Alimudin Sandora, Desta Sandra, Shifa Saputra, Rama Arsalta Bara Saputri, Nur Ayu Saskya Ramadhanti Saswiyanti, Enny Sendora, Desta Septi Wahyu Lestari Septiana, Ana Sevira Nur Azmi Sevira Nur Azmi Shifa Sandra Sidik, Mahfud Sinurat, Lidya Septaria Soegiharto, Yolande Cathleya Sri Retnaning Rahayu, Sri Retnaning Suhada Suhada, Suhada Suharto, Ignatius Sumaryo Sunandar Sunandar Sunandar Sunandar Supriyadi, Tatang Suratman Umar Susandi, Waryoko Susanti, Iproh Susanto, Alvin Wiwiet Susilawati Suwarno Syarif Hidayat Amrullah Teddy TRIANDIZA Thesalonika, Natasya Tugiyono Tugiyono Tugiyono Tugiyono Tugiyono Utoyo, Laji Vega, Cindy Ameliya Vidya Kurniasih Vidyanti Kurniasih Virnarenata, Elsa Wahyono, Agus Waryoko Susandi WINARNO Winarno Winarno Yani, Ani Andri Yanti Yulianti Yogi Aprio Yuliana Andriyani Yuni Tinasari Yunior, Elfita Nova Zacky Zeinel Ibad Muchlas Zamroni