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Learning Bats Handling For Oral Swab Sampling Azmi, Sevira Nur; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Saswiyanti, Enny; Srihanto, Eko Agus; Pratiwi, Dian Neli; Susandi, Waryoko
Jurnal Medisci Vol 3 No 1 (2025): Vol 3 No 1 August 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/medisci.v3i1.1014

Abstract

Background. The only flying mammals, bats, play an essential role in their natural habitat. Based on the type of food, bats can be divided into two groups: fruit-eating bats and insect-eating bats. Bats rank second-highest in species diversity, with 1,439 worldwide and 239 in Indonesia. Known as reservoirs of viruses, including coronaviruses. Aims. Bats are suspected to be related to COVID-19. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) University of Lampung 2024-2025, and in collaboration with the Lampung Disease Investigation Centre, learning the procedures for handling bats properly so as not to physically harm bats in oral swab sampling and species recognition in Braja Harjosari, directly next to Way Kambas National Park was done. Methods. The life-trapping technique uses a mist net. Bat handling for taking oral swab was carried out using the pinch grip method, which is holding both arms of the bat backwards utilizing the thumb and middle finger with the bat positioned facing upwards. Conclusion. The bat's mouth is then blown open and a cotton swab is gently inserted into the bat's mouth. Afterwards, the bat is rested, given water drops, and released into nature. Oral swab samples from 10 individual bats: frugivorous bats, Cynopterus brachyotis (n = 8), Cynopterus horsfieldii (n = 1), and insectivorous bat, Scotophilus kuhlii (n = 1).
Analysis Of Cadmium (Cd) And Lead (Pb) Levels In Water Samples By Atomic Absorption Spectrophotometry (AAS) At The Lampung Health Laboratory Center Adinda, Calesta; Rustiati, Elly Lestari; Susanti, Iproh; Cicilia, Okferina
Jurnal Improsci Vol 3 No 4 (2026): Vol 3 No 4 February 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/improsci.v3i4.1053

Abstract

Background. For sanitary hygiene, water is used for maintaining personal hygiene, such as bathing and brushing, as well as for food washing, dishwashing, and laundry in daily life, so its quality differs from that of drinking water. This study focuses on heavy metal pollution, particularly lead (Pb) and cadmium (Cd), which are known toxic substances harmful to living organisms. Aims. Its purpose is to determine the levels of cadmium and lead in water samples by atomic absorption spectrophotometry at the Lampung Health Laboratory Center, conducted in December 2024-January 2025. T Methods. The method refers to SNI No. 6989.84 of 2019 for 78 samples of sanitary hygiene water by Atomic Absorption Spectrophotometry (SSA). Cadmium (Cd) analysis of 37 samples: 21 were positive for Cd, but none exceeded the threshold limit value of 0.003 mg/L. Conclusion. Meanwhile, the results of the lead (Pb) analysis showed that 1 of 37 samples did not exceed the threshold limit value of 0.01 mg/L, as stipulated in the Indonesian Minister of Health Regulation No. 2 of 2023.
Learning Bats Handling for Oral Swab Sampling Azmi, Sevira Nur; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Saswiyanti, Enny; Srihanto, Eko Agus; Pratiwi, Dian Neli; Master, Jani; Susandi, Waryoko
Jurnal Agrosci Vol 3 No 4 (2026): Vol 3 No 4 March 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i4.1154

Abstract

Background. The only flying mammals, bats play an essential role in their natural habitat. Based on the type of food, bats can be divided into two groups: fruit-eating bats and insect-eating bats. Bats rank second-highest in species diversity, with 1,439 worldwide and 239 in Indonesia. Known as reservoirs of viruses, including coronaviruses. Bats are suspected to be related to COVID-19. Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) University of Lampung 2024-2025, and in collaboration with the Lampung Disease Investigation Centre, learning the procedures for handling bats properly so as not to physically harm bats in oral swab sampling and species recognition in Braja Harjosari, directly next to Way Kambas National Park was done. The life-trapping technique uses a mist net. Methods. Bat handling for taking an oral swab was carried out using the pinch grip method, holding both bat arms backwards with the thumb and middle finger, with the bat positioned facing upwards. Conclusion. The bat's mouth is then blown open, and a cotton swab is gently inserted into the bat's mouth. Afterwards, the bat is rested and given a drink/water and released into nature. Oral swab samples from 10 individual bats, fruit-eating bats, Cynopterus brachyotis (n = 8), Cynopterus horsfieldii (n = 1), and an insect-eating bat, Scotophilus kuhlii (n = 1).
RNA Isolation from Oral Swab Sample On Bats of Rural Area, Braja Harjosari, East Lampung Kurniasih, Vidyanti; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Saswiyanti, Enny; Pratiwi, Dian Neli
Jurnal Agrosci Vol 3 No 3 (2026): Vol 3 No 3 January 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i3.1161

Abstract

Background. Bats are considered animals that carry infectious diseases, are known to be reservoirs of viruses, and are suspected of causing disease outbreaks. The World Health Organization stated that this disease outbreak is caused by Coronavirus Disease 2019 (COVID-19), which is caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) and in collaboration with the Lampung Disease Investigation Center, a preliminary study on EID detection on bats based on Predict Protocols was done to identify the presence of coronavirus on bats, including rural areas, Braja Harjosari, East Lampung. It is located next to Way Kambas National Park. Methods. Oral swab samples were taken from ten bats, 3 species: Cynopterus brachyotis and Cynopterus horsfieldi, and Scotophilus kuhlii. RNA extraction was conducted by Predict Protocols, which has four stages: lysis, binding, washing, and elution. Conclusion. Two samples showed thin-band luminescence. While in the quantity test, there are three samples of good concentration results between 1.8 and 2.0.
Pelatihan Olah Sayur Hasil Kebun: Pembuatan Es Krim Berbasis Sayuran Di PT Great Giant Food, Terbanggi Besar, Lampung Tengah Elly Lestari Rustiati; Susilawati; Agus Subagyo; Putri Dwi Astuti; Fauzan Khumaidi; Dewi Puspita Widiarini
AMMA : Jurnal Pengabdian Masyarakat Vol. 4 No. 4 : Mei (2025): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A training program on processing local vegetables into vegetable-based ice cream was conducted at PT Great Giant Food (GGF), Central Lampung, aimed at empowering women's groups to enhance the value of local agricultural products. The training involved 32 participants from the GGF Employees' Wives Association and the Tulip Women Farmers Group. Activities included nutrition education, demonstrations, product tasting, and hands-on practice in making ice cream using pakcoy and bokcoy. A participatory and practical approach encouraged active engagement, improved technical skills, and fostered flavor innovation. The results showed that participants successfully produced vegetable ice cream with favorable organoleptic qualities. This initiative not only promoted local resource utilization but also strengthened women's economic roles and aligned with sustainable development goals. The training model presents strong potential for replication in similar community contexts.
ONLINE AND SEMI-ONLINE MENTORING METHODS: COMMUNITY EMPOWERMENT STRATEGIES DURING THE NEW NORMAL PANDEMIC Elly Lestari Rustiati; Elsa Virnarenata; Priyambodo Priyambodo; Salih Alimudin; Dwi Asmi; Edi Santoso; Yanti Yulianti; Dian Neli Pratiwi; Erdi Suroso
Jurnal Abdisci Vol 1 No 5 (2024): Vol 1 No 5 Tahun 2024
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/abdisci.v1i5.207

Abstract

 Community stewardship can be done directly. Direct interaction is commonly applied, appreciated, and easy to communicate. During the COVID-19 pandemic, direct interaction and visits are almost impossible due to activity limitations and social distancing. Online and semi-online interaction can be applied by inviting community limited representatives combined with the online method. Initiation on online communication is introduced. This project was conducted in companionship and training in Braja Harjosari and Labuhan Ratu VII, East Lampung. Following the introduction of the online and semionline methods, local people can apply and share their knowledge of the new normal paradigm.
TRAINING ON EDU-AGROTOURISM FINANCIAL GOVERNANCE OF KWT BINA PERTANI, KAMPUNG LIMAN BENAWI, TRIMURJO, CENTRAL LAMPUNG Sri Retnaning Rahayu; Elly Lestari Rustiati; Dian Neli Pratiwi; Ana Septiana; Muhammadi Ridho Al Amin; Angga Kurniawan
Jurnal Abdisci Vol 1 No 8 (2024): Vol 1 No 8 Tahun 2024
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/abdisci.v1i8.326

Abstract

A training session on Edu-agrotourism financial governance was conducted for KWT Bina Pertani, a farmer women's group in Kampung Liman Benawi, Trimurjo, Central Lampung, on January 26, 2024. The primary objective of this fundamental accounting and finance training was to enhance the understanding of financial management and the skills of KWT Bina Pertani members and village civil servants. The curriculum encompassed an overview of financial statements, rudimentary bookkeeping, the compilation of financial statements, and strategies for crop pricing. The training objectives were achieved through coordination, agreement, training materials and equipment preparation, and activities and evaluations. The training was delivered face-to-face with direct practice, and the participants enthusiastically received it. The training session yielded positive outcomes, with successful material delivery and achievement of the activity objectives.
Detection of Environmental DNA from Tree Bark Associated with Rubbing Behavior of Dicerorhinus sumatrensis in Way Kambas National Park Priyambodo; Zulkarnain, Danisworo; Rustiati, Elly Lestari; Kurniawati, Yeyen; Putri, Chicka Refina Rahma; Srihanto, Eko Agus; Saswiyanti, Enny; Arsan, Zulfi; Sukatmoko; Pratiwi, Dian Neli; Permatasari, Nindy
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/zv2m2645

Abstract

The development of non-invasive methods is essential for monitoring critically endangered species such as the Dicerorhinus sumatrensis. This study aimed to evaluate the potential of tree bark associated with rubbing behavior as a source of environmental DNA (eDNA) for species detection in Way Kambas National Park. A total of 30 samples were collected from tree bark surfaces showing evidence of rubbing activity. DNA was extracted and analyzed using two approaches: simple electrophoresis and polymerase chain reaction (PCR)-based molecular methods. The results showed that the simple method failed to detect visible DNA bands, indicating low DNA concentration and degradation in environmental samples. In contrast, PCR amplification successfully detected DNA in 5 out of 30 samples, including hair-derived and epithelial residues associated with rubbing behavior.containing hair residues produced more reliable detection compared to those with only epithelial traces. These findings demonstrate that tree bark able to serve as a potential non-invasive source of eDNA, particularly when analyzed using sensitive molecular techniques. This approach provides an alternative method for detecting elusive species and contributes to the development of effective conservation strategies for the sumatran rhinoceros.
Co-Authors 'Aliyah, Siti Hamidatul A.Basyir Firdaus Adelia, Ersa Imelda Adi, Marcelus Adinda, Calesta Afandi, Aril Agus Setiawan AGUS SETIAWAN Agus Setiawan Agus Subagyo Alfandi, Aril Alvin Wiwiet Susanto Alvin Wiwiet Susanto Ana Septiana Angga Kurniawan Angga Pramudya Angga Pramudya Anggy, Fransiska Panasea Apriani, Vivin Apriliasari, Messy Aprio, Yogi Aril Afandi Arsan, Zulfi Aryani, Nina Yudha Ashari, Minanti Mayda Azmi, Sevira Nur Azzahra, Laila Salwa Bondan Pergola Cicilia, Okferina Cindy Yoeland Violita Cindy Yoeland Violita Daely, Sofia Vao Afni Dewi Puspita Widiarini Diah Esti Anggraini Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dina Farida Utami Dina Farida Utami Dwi Asmi Edi Santoso Edi Santoso Edo Firnanda Edo Firnanda, Edo Eka Sulpin Ariyanti Eka Sulpin Ariyanti, Eka Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto, Eko Agus Elfita Nova Yunior Elsa Virnarenata Elsa Virnarenata Emantis Rosa Enny Saswiyanti Enny Saswiyanti Erdi Suroso Erdi Suroso Fahrezi, Aditya Fauzan Khumaidi Febriansyah, Muhammad Febriyani, Dwi Ayu Feni Yulinda Firwantoni Firwantoni Firwantoni, Firwantoni Fitrisyah, Asyifa Zahara G. Gunawan Gina Dania Pratami, Gina Dania Haryanto, Sugeng Prayitno Heni, Ahyul Hidayatulloh I Gede Swibawa Ibransyah, Rasyid Ifan Wahyudi Ignatius Suharto Imtitsal, Aulia Indah Ayu Lestari Indah Ayu Lestari, Indah Ayu Intan Diani Fardinatri Iqbal Amiruddin Ihsanu Iqbal Amiruddin Ihsanu Irawati, Anie Rose IRFAN NURARIFIN, IRFAN Irwan Nasution Jani Master Jani Master, Jani Kasturi Khalil, Abdul Rouf Amarulloh Krismuniarti, Elisabeth Devi Kurniasih, Vidyanti Kurniawati, Yeyen Kusuma Handayani, Kusuma Kusuma, Andriyani Wijaya Lestari, Septi Wahyu Maharani, Annisa Lidya Marcelus Adi Minanti Mayda Ashari Muhammad Febriansyah Muhammad Rohiyan Muhammad Rohiyan Muhammadi Ridho Al Amin Natasya Thesalonika Neli Pratiwi, Dian Novianasari, Tika Nuning Nurcahyani Panasea Anggy, Fransiska Parabi, M. Iqbal Pawaka, Arrahmaan Syah Permatasari, Nindy Pratama, M. Idris Afta Pratiwi, Dian Neli Pratiwi, Ratih - Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Putri Dwi Astuti Putri, Chicka Refina Rahma Raden Mohamad Herdian Bhakti Ramadan, Viki Ramadhan, Viki Ramadhanti, Saskya Adrila Rhamadaningtyas, Nabila Aulia Rodliyah, Aini Robby Romaya Wulan Suciningtyas Rosa, Emantis Sa'uddah, Lousanja D. Safitri, Anggi Salih Alimudin Salih Alimudin Sandra, Shifa Saputra, Rama Arsalta Bara Saputri, Nur Ayu Saskya Ramadhanti Saswiyanti, Enny Septi Wahyu Lestari Sevira Nur Azmi Shifa Sandra Sidik, Mahfud Sinurat, Lidya Septaria Soegiharto, Yolande Cathleya Sri Retnaning Rahayu Suhada Suhada, Suhada Suharto, Ignatius Sukatmoko Sunandar Suratman Umar Susandi, Waryoko Susanti, Iproh Susanto, Alvin Wiwiet Susilawati Syarif Hidayat Amrullah Teddy TRIANDIZA Thesalonika, Natasya Tugiyono Tugiyono Tugiyono Tugiyono Tugiyono Utoyo, Laji Vega, Cindy Ameliya Vidyanti Kurniasih Virnarenata, Elsa WINARNO Winarno Winarno Yani, Ani Andri Yanti Yulianti Yanti Yulianti Yogi Aprio Yuliana Andriyani Yuni Tinasari Yunior, Elfita Nova Zacky Zeinel Ibad Muchlas Zamroni Zulkarnain, Danisworo