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All Journal HAYATI Journal of Biosciences VALENSI Bionatura Teknotan: Jurnal Industri Teknologi Pertanian Jurnal Natur Indonesia Microbiology Indonesia Majalah Kedokteran Bandung Indonesian Journal of Pharmaceutical Science and Technology LEMMA: Letters Of Mathematics Education Indonesian Journal of Biotechnology Jurnal Elemen Edumatsains QALAMUNA: Jurnal Pendidikan, Sosial, dan Agama Health Notions Jurnal Biodjati JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Chimica et Natura Acta Jurnal Moral Kemasyarakatan JURNAL SAINS INDONESIA IndoMath: Indonesia Mathematics Education Jurnal Kimia Terapan Indonesia Jurnal Pendidikan Kimia (JPKim) Indonesian Journal of Chemistry IJCER (International Journal of Chemistry Education Research) Jurnal PAJAR (Pendidikan dan Pengajaran) Gelanggang Olahraga: Jurnal Pendidikan Jasmani dan Olahraga (JPJO) JOURNAL SPORT AREA FLUIDA Molekul: Jurnal Ilmiah Kimia Jurnal Pedagogik Olahraga Journal of Authentic Research on Mathematics Eduacation (JARME) Jurnal PORKES Physical Activity Journal (PAJU) JUMORA: Jurnal Moderasi Olahraga JES-MAT (Jurnal Edukasi dan Sains Matematika) Jurnal Ilmiah Berkala Sains dan Terapan Kimia Pharmaceutical Sciences and Research (PSR) Rangkiang Mathematics Journal International Journal of Educational Research Excellence (IJERE) Jurnal Sains dan Teknologi Farmasi Indonesia Euclid al Kimiya : Jurnal Ilmu Kimia dan Terapan ACTIVE: Journal of Physical Education, Sport, Health and Recreation Journal of Physical Education Health and Sport

Toto Subroto

Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran

Author-ID : 387376

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Perancangan Vaksin Virus Papilloma Manusia Tipe-16 Berbasis Epitop dengan Berbantukan Imunoinformatika Opik Taupiqurrohman; Muhammad Yusuf; Sukma Nuswantara; Toto Subroto
Jurnal Pendidikan Kimia (JPKim) Vol 8, No 3 (2016): Desember
Publisher : Pascasarjana Universitas Negeri Medan

Abstrak. Sampai saat ini vaksinasi dianggap sebagai cara yang efektif dalam mencegah penyakit menular. Semua proses vaksinasi bekerja dengan menampilkan antigen asing terhadap sistem imun agar respon sistem imun bangkit. Bahan aktif vaksin dapat berbentuk patogen utuh (bakteri atau virus) yang dilemahkan, atau komponen patogen yang imunogenikdantelah dimurnikan. Pendekatan konvensional dalam pengembangan vaksin membutuhkan biakan mikroorganisme patogen dan diseksi dengan menggunakan teknik biokimia, imunologi, dan metode mikrobiologi dalam mengidentifikasi komponen penting terhadap sistem imunitas. Metode ini telah berhasil dalam berbagai kasus, namun gagal memberikan solusi bagi banyak patogen yang belum dapat dibuatkan vaksinnya. Dewasa ini, dimungkinkan menggunakan informasi genomik untuk mempelajari perancangan vaksin secara in silico, tanpa perlu membiakan mikroorganisme patogen. Pendekatan yang telah diberi nama 'vaksinologi terbalik' ini dapat mengurangi waktu yang dibutuhkan untuk identifikasi kandidat vaksin dan memberikan solusi baru bagi vaksin-vaksin yang selama ini sulit dikembangkan.Meningkatnya pemahaman mengenai pengenalan antigen pada tingkat molekul, telah berdampak pada pengembangan desain rasional vaksin berbasis peptida atau epitop. Konsep vaksin peptida didasarkan pada identifikasi dan sintesis kimia epitop sel-B dan sel-T yang imunodominan, yang dapat menginduksi respon imun spesifik. Perkembangan yang cepat dari teknik bioinformatika dan aplikasinya dengan sejumlah besar data eksperimen telah melahirkan bidang baru, disebut imunoinformatika. Imunoinformatika adalah cabang dari bioinformatika yang berkaitan dengan analisis in silico serta pemodelan data imunologi dan masalahnya. Kajian ini bertujuan untuk merancang vaksin virus papilloma manusia berbasis epitop berbantukan imunoinformatika. Kata Kunci: vaksin, virus papilloma manusia, bioinformatika, imunoinformatika, epitope

Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress Saronom Silaban; Murniaty Simorangkir; Shabarni Gaffar; Iman Permana Maksum; Toto Subroto
Jurnal Pendidikan Kimia (JPKim) Vol 11, No 3 (2019): December
Publisher : Pascasarjana Universitas Negeri Medan

Many proteins produced in E. coli accumulate in inclusion bodies. This study aims to detect the role of temperature in reducing the formation of inclusion bodies during recombinant human prethrombin-2expressed in E. coli BL21 (DE3) Arctic Express host. In this study, we created a host growth curve to find out the right time to add an inducer. The inducer used in our experiment was IPTG 0.1 mM. The fermentation process use a temperature of 12°C and 22°C. The results showed that recombinant human prethrombin-2 was successfully expressed as protein soluble using an optimum temperature of 12°C in E. coli BL21 (DE3) Arctic Express. It was indicated from the 63kDa protein band obtained from the soluble fraction on SDS-PAGE. The higher temperature of fermentation increased the amount of protein in the insoluble fraction due. It concluded that the fermentation temperature affect the rate of prethrombin-2 expression.Keywords: E. coli BL21(DE3) ArcticExpress, prethrombin-2, soluble, temperature

Combination of Genetic Manipulation Improved Saccharomycopsis fibuligera α-Amylase Secretion by Pichia pastoris Shabarni Gaffar; Dessy Natalia; Toto Subroto; Oo Suprijana; Soetijoso Soemitro
Indonesian Journal of Chemistry Vol 19, No 2 (2019)
Publisher : Universitas Gadjah Mada

This study assessed the combinations of genetic manipulation; signal peptide modification, gene dosage increment and co-expression of folding component, to increase Saccharomycopsis fibuligera R64 α-amylase (Sfamy) secretion in Pichia pastoris. Sfamy native signal peptide was replaced with modified signal peptide which contained 15 amino acid of mouse salivary α-amylase signal peptide fused to the pro-region of the signal peptide of Saccharomyces cerevisiae α-mating factor (α-MF). Increase in gene dosage was identified by screening for P. pastoris harboring multicopies of the Sfamy gene. Whereas, co-expression of folding component was done by addition of Protein Disulfide Isomerase (PDI). Expression plasmids harboring Sfamy containing modified signal sequence (pPICZA-MS-Sfamy) was used to transform P. pastoris GS115, and gene dosage increment was screened using zeocin. Effect of PDI co-expression on secretion levels of Sfamy was assessed by constructing the pPIC3.5K-Pdi1 plasmid and introducing into P. pastoris harboring multicopies of MS-Sfamy for expression of Sfamy. Signal peptide modification consequently increased Sfamy secretion by P. pastoris by 3.3-fold compared to native signal peptide. Gene dosage increment had improved Sfamy secretion by 11-fold in P. pastoris [MS-Sfamy] resistant to 2000 μg/mL zeocin, compared to P. pastoris harboring one copy of WT-Sfamy. Hence, PDI co-expression increased the secretion of Sfamy by 2-fold as compared without PDI co-expression. In summary, the combination of genetic manipulation successfully increased Sfamy secretion by 20-fold compared to P. pastoris harboring one copy of WT-Sfamy.

An Electrochemical Aptasensor for the Detection of HER2 as a Breast Cancer Biomarker Based on Gold Nanoparticles-Aptamer Bioconjugates Yeni Wahyuni Hartati; Sari Syahruni; Shabarni Gaffar; Santhy Wyantuti; Muhammad Yusuf; Toto Subroto
Indonesian Journal of Chemistry Vol 21, No 6 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.67124

Inaccurate diagnoses contributes to the high mortality rate of breast cancer. Human epidermal growth factor receptor 2 (HER2) is overexpressed in breast cancer tumors at around 20–30%. This study aims to develop an electrochemical biosensor for HER2 based on a gold nanoparticle-aptamer bioconjugate (AuNP@HER2 aptamer) and investigate the interaction between DNA aptamer and HER2 using computational methods. The bioconjugate was synthesized using maleimide and polyethylene glycol as a linker. The –NH2 group of cysteamine that modified the gold electrode can form a covalent bond with the bioconjugate maleimide. The interaction of the bioconjugated aptamer with HER2 was measured electrochemically based on the [Fe(CN)6]3−/4− redox system. The limit of detection, the linear range of HER2, precision, and accuracy in this study were 1.52 ng mL–1, 0.01 to 15.0 ng mL–1, 0.1298, and 94.06%, respectively. The structure of the DNA aptamer was modeled using mFold, Assemble2, and Chimera, with the interaction between the DNA aptamer and HER2 explored by NPDock. The modeling of the aptamer with HER2 showed that electrostatic interactions dominated the attractive forces. The resulting interaction pattern can be used as a template to improve the binding energy of the aptamer, thus providing insight into the development of aptamer-based biosensors.

SINTESIS PEPTIDA P251-9 BERGUGUS PELINDUNG DENGAN METODE SINTESIS PEPTIDA FASA PADAT FMOC/TBU MENGGUNAKAN ADITIF OKSIMA Ari Hardianto; Toto Subroto; Unang Supratman
Jurnal Sains dan Terapan Kimia Vol 5, No 2 (2011)
Publisher : Program Studi Kimia, Universitas Lambung Mangkurat

Peptida P25 merupakan epitop yang dapat dengan mudah mengaktivasi sel T penolong. Sel T penolong teraktivasi berperan dalam aktivasi sel B untuk membentuk antibodi. Peptida bahkan protein dapat dibuat dengan metode sintesis peptida fasa padat Fmoc/tBu menggunakan aditif HOBt. Namun sayangnya HOBt memiliki karakter yang mudah meledak. Pada penelitian ini telah berhasil disintesis bagian epitop P25 (peptida P251-9 bergugus pelindung) yang memiliki tingkat kemurnian tinggi dan perolehan 36,4% dengan metode sintesis peptida fasa padat Fmoc/tBu menggunakan aditif oksima pengganti HOBt. Kata kunci: Epitop, P251-9, sintesis peptida fasa padat Fmoc/tBu, oksima.

Implementation of augmented reality-assisted learning media on three-dimensional shapes Surya Amami Pramuditya; Sri Pitriayana; Toto Subroto; Risnina Wafiqoh
Jurnal Elemen Vol 8, No 2 (2022): July
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/jel.v8i2.5238

This research is motivated by the low understanding of students in studying the object of geometry study. The media that had previously been used in the form of a spatial framework made of bamboo or iron turned out to take up quite a lot of space and was not practical to carry in large quantities. Therefore, efficient and practical media are needed to help visualize concrete objects in learning. This research aims to implement Augmented Reality (AR)-assisted learning media on the material of three-dimensional shapes. This study uses an explanatory qualitative method. The participant in this study was one student from one of the junior high schools in the Cirebon Regency. Data collection techniques used are through interviews and documentation. The analysis technique consists of three stages: data reduction, data presentation, and concluding. The results showed that the AR application could make it easier for the student to understand the material and solve the problem of three-dimensional shapes. However, the student still has a little difficulty using the application and is a little confused when identifying the parts of the cube because the image of the shape raised by the application is still not clear. This research implies that AR applications can make students' spatial abilities better.

Implementasi Model Pembelajaran Pada Olahraga Permainan Bolatangan Rochmat Rahayu; Toto Subroto; Didin Budiman
Physical Activity Journal (PAJU) Vol 1 No 2 (2020): Physical Activity Journal (PAJU)
Publisher : Department of Physical Education, Faculty of Health Sciences, Universitas Jenderal Soedirman

Peer teaching adalah sebuah model pembelajaran yang dapat mengembangkan aspek afektif seperti kerjasama, komunikasi dan mengembangkan rasa percaya diri sehingga Jumlah Waktu Aktif Belajar siswa dapat meningkat. penelitian ini bertujuan untuk mengidentifikasi peningkatan Jumlah Waktu Aktif Belajar (JWAB) dalam pembelajaran bolatangan dengan menerapkan medel pembelajaran Peer Teaching. Metode menggunakan Penelitian Tindakan Kelas (PTK), di SMA AL-Musyawarah Lembang, dengan sampel 33 orang dan terdiri dari 14 siswa putra dan 19 siswa putri. Hasil penelitian secara umum menunjukan siswa bergerak secara aktif sehingga meningkatkan Jumlah Waktu Aktif Belajar (JWAB) yang ditunjukan dari motivasi tinggi pada saat terlibat dalam pembelajaran permainan bolatangan. Tutor dan siswa dapat meningkatkan kemampuan berkomunikasi dan berinteraksi dengan orang lain. Kesimpulan penelitian bahwa model peer Teaching dapat gunakan pada pembelajaran bolatangan, karena secara bertahap dapat meningkatkan nilai kerjasama, komunikasi, menyampaikan informasi dan mengembangkan rasa percaya diri sehingga Jumlah Waktu Aktif Belajar (JWAB) sebagai indikator bahwa siswa terlibat secara aktif selama pembelajaran juga meningkat.

Studi Mutasi Titik A3243G DNA Mitokondria Penyebab Maternally Inherited Diabetes and Deafness Sriwidodo, Sriwidodo; Suprijana, O; Subroto, Toto; Maksum, Iman Permana
Majalah Ilmu Kefarmasian Vol. 5, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Point mutation of mitochondrial DNA A3243G has been known as a cause of Mater-nally Inherited Diabetes and Deafness (MIDD). Potency of MIDD can be identified from patient phenotype of Non Insulin Dependent Diabetes Mellitus (NIDDM). The objective of this study is acquiring information about MIDD on patient of NIDDM type and obtaining the simple method to detect the point mutation of mtDNA A3243G. 50 NIDDM patients were attained from RSCM Hospital, Jakarta. Information con-cerning family history with NIDDM and existences of deafness, medication, and other complication and manifestation were obtained through interview and ques-tioner. Point mutation of A3243G was determined with the method of PCR Allele’s Specific Amplification (PASA) Mismatch 2 bases and PCR-Restriction Length Poly-morphism (PCR-RFLP) with the HaeIIl restriction enzyme. Detectable Potency MIDD was found by perceiving the patient phenotype and identifying the mutation of heteroplasmic A3243G utilizing the PASA method.

Early development of self‐administered COVID‐19 rapid test based on nucleocapsid detection in saliva sample Siti Soidah; Toto Subroto; Sari Syahruni; Fauzian Giansyah; Henry Chandra; Dhiya Salsabila; Bachti Alisjahbana; Nisa Fauziah; Hesti Lina Wiraswati; Leonardus Wiydatmoko; Basti Andriyoko; Anita Yuwita; Muhammad Yusuf
Indonesian Journal of Biotechnology Vol 27, No 3 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.72269

More than 6,000,000 people have died due to the coronavirus (COVID‐19) pandemic. This disease spread quickly due to its highly contagious nature. The SARS‐CoV‐2 virus that causes the disease can be transmitted through saliva droplets secreted by infected people at a distance of less than 1 m. As a result, saliva has been accepted as an alternative specimen for COVID‐19 detection by the Centers for Disease Control and Prevention (CDC). Furthermore, WHO recommended the use of rapid antigen tests based on lateral flow immunoassay when reverse transcription‐polymerase chain reaction (RT‐PCR) is not available. We developed a saliva‐based rapid antigen test by optimizing the antibody concentration and optimum pH for the conjugation of antibody and gold nanoparticles. We found that the best running buffer formulation consisted of 75 mM sodium phosphate buffer, 1% NaCl, 1% Triton X‐100, 0.5% N‐acetyl‐L‐cysteine, and 0.02% sodium azide. The addition of a mucolytic agent in the buffer can reduce the viscosity of saliva, thus improving sensitivity. The rapid test developed detected the lowest concentration of nucleocapsid protein at 0.1 μg/mL. Our study revealed 100% specificity against negative COVID‐19 saliva and no cross‐reaction with avian influenza virus hemagglutinin.

Optimization of Aptamer-Based Electrochemical Biosensor for ATP Detection Using Screen-Printed Carbon Electrode/Gold Nanoparticles (SPCE/AuNP) Rahmaniar Mulyani; Nida Yumna; Iman Permana Maksum; Toto Subroto; Yeni Wahyuni Hartati
Indonesian Journal of Chemistry Vol 22, No 5 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.72820

Electrochemical biosensors are used to detect adenosine triphosphate (ATP) levels, which are involved in a variety of biological processes, such as regulating cellular metabolism and biochemical pathways. Therefore, this research aims to develop an aptamer-based electrochemical biosensor with Screen Printed Carbon Electrode/gold nanoparticles (SPCE/AuNP) and collect data as well as information related to ATP detection. The modification of SPCE with AuNP increased the analyte’s binding sensitivity and biocompatibility. The aptamer was selected based on its excellent bioreceptor characteristics. Furthermore, aptamer–SH (F1) and aptamer-NH2 (F2) were immobilized on the SPCE/AuNP surface, which had been characterized using SEM, EIS, and DPV. Also, the ATP-binding aptamers were electrochemically characterized using the K3[Fe(CN)6] redox system and Differential Pulse Voltammetry (DPV). According to the optimization results using the Box-Behnken experimental design, the ideal conditions obtained from the factors influencing the experiment were the F1 concentration and incubation time of 4 µM and 24 h, respectively, as well as F1/F2/ATP incubation time of 7.5 min. Meanwhile, for the range of 0.1 to 100 µM, the detection (LoD) and quantification (LoQ) limits were 7.43 and 24.78 µM, respectively. Therefore, this aptasensor method can be used to measure ATP levels in real samples.

Co-Authors Abdul Alim Kahari, Abdul Alim Abu Bakar M.I. Syihab Ade Rizki Ridwan Firdaus Agus Gumilar Ahmad Fariz Maulana Alifa I. Nabila Amiruddin, Mohd. Hasril Angga Himas Setyawan Anita Yuwita Anneke Noviyanti Aprillia, Mutiara Sukma Ari Hardianto Bachti Alisjahbana Bacthi Alisjahbana Basti Andriyoko Bevi Lidya Bevi Lidya Bisri Musthofa Budi Santoso Budiana, Dian Budiantoro, Ogi Cindy Aulia Sari D Agus Yusuf Wildan Dani Permana Debby M Sumanti Defi Supriyati, Ayu DESSY NATALIA DESSY NATALIA DESSY NATALIA Desy Linasari Dewi Astriany Dewi Ratih Handayani Dhiya Salsabila Diana P Rahmawati Diana Rakhmawaty Eddy Didi Suryadi Didin Budiman, Didin DWI RAHMAWATI Dzakiyah 'Izzah Ghassani Adsumi Effendi, Syulastri EKO YULIANTO Elazmanawati Lembong Fadhilah, Muhammad Naufal Fadhlillah, Muhammad Fadillah, Lukky Farihandiandra, Yusron Fathurrohman, Muhammad Andi Fatin, Nuri Nuraini Fauzian Giansyah Febriani Aji Kusuma, Sherlynda FERNITA PUSPASARI Firdaus, Muhammad Lutfi Gifa Nur Arofah Gifa Nur Arofah Gumelar, Abih Gumilar, Agus Hafizatunnisa, Hafizatunnisa Hambali, Burhan Hamdiyanti, Meti Henry Chandra Hesti Lina Wiraswati Idar Idar Idar Idar Idar Idar Idar, Idar Iman Permana Maksum Iman Rahayu Kharimah, Nurul Ikhsan KHOMAINI HASAN Khomaini Hasan Khomairi Hasan Korry Novitriani Korry Novitriani, Korry Kusumawardhani, Shinta Laelasari Laelasari , Laelasari Laelasari Laelasari Laelasari Laelasari, Laelasari Leonardus Wiydatmoko Lesmana, Gianjar Lukky Fadillah Mamlikatu Ilmi Azizah Meti, Meti Hamdiyanti Mony, Siti Rasnawati Muchamad Subali Noto Muhamad Abidin Muhamad Zulchoir Rasyid Muhammad Fadhil Z, Rusdi Noor Rosa (p:43-50) Muhammad Fadhlillah Muhammad Hilman Daniswara Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Murniaty Simorangkir Murniaty Simorangkir Naufal D, Kevin Nida Yumna Niko, Ade Nisa Fauziah Noviyanti, Anneke Nurfairuz, Hasna Siti Nurmalasari, Ratna Nurul Aida Fathya Nurul Ikhsan Karimah Nurul Ikhsan Karimah O Suprijana O Suprijana O Suprijana, O Ogi Budiantoro OGI BUDIANTORO Oo Suprijana Opik Taupiqurrohman Opik Taupiqurrohman Pertiwi, Wulan Praja, Ena Suhena Purba Upay Rahmaniar Mulyani Rahmaniar Mulyani Rahmat, Alit Ratna Yulistya Ningrum Regi Dwi Septian Riezki Amalia Rima Handiyani Risnina Wafiqoh Rizky Rosjanuardi Rochmat Rahayu Rodiana , Ivta Rodiana, Ivta Safri Ishmayana Safrl Ismayana Sahroni Sahroni Sahroni, Sahroni Santhy Wyantuti Sari Agung, Syennie Sari Syahruni Sari Syahruni Saronom Silaban Saronom Silaban SARONOM SILABAN Saronom Silaban Setyaningrum, Sinta Shabarni Gaffar Shinta Kusumawardani Siti Nuraini Siti Soidah Siti Umayah Siti Umayah, Siti Soetijoso Soemitro SOETIJOSO SOEMITRO Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Sofyan Multazam N Aji Sri Asnawati Sri Pitriayana Sriwidodo Sriwidodo Sriwidodo Sriwidodo Sukma Nuswantara Sukma Nuswantara Sukma Nuswantara Sunan M. Alpiansyah Surya Amami Pramuditya Sutarya Enus Sutarya Enus SUTARYA ENUS Sutarya Enus Syani, Mamay Syfa Nurfadilah Syfa Nurfadilah Taupiqurrohman, Opik Taupiqurrohman, Opik Tika Pradnjaparamita, Tika Tonah Tonah Triana N Meirina Umi Baroroh Umi Baroroh Unang Supratman Upay, Purba wahyu widayat Wanda Destiarani WANGSA TIRTA ISMAYA Wildatus Sholihah Windy Aulia Putri Wulan Pertiwi Yani Suryani Yeni W Hartati Yeni Wahyuni Hartati Zakiyyah, Salma Nur

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