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All Journal VALENSI Bionatura Teknotan: Jurnal Industri Teknologi Pertanian Jurnal Natur Indonesia Microbiology Indonesia Majalah Kedokteran Bandung Indonesian Journal of Pharmaceutical Science and Technology LEMMA: Letters Of Mathematics Education Indonesian Journal of Biotechnology QALAMUNA: Jurnal Pendidikan, Sosial, dan Agama Jurnal Biodjati JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Chimica et Natura Acta Jurnal Moral Kemasyarakatan JURNAL SAINS INDONESIA Jurnal Kimia Terapan Indonesia Jurnal Pendidikan Kimia (JPKim) Indonesian Journal of Chemistry Jurnal PAJAR (Pendidikan dan Pengajaran) Gelanggang Olahraga: Jurnal Pendidikan Jasmani dan Olahraga (JPJO) Molekul: Jurnal Ilmiah Kimia Jurnal Pedagogik Olahraga Journal of Authentic Research on Mathematics Eduacation (JARME) Jurnal PORKES JUMORA: Jurnal Moderasi Olahraga Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Pharmaceutical Sciences and Research (PSR) Rangkiang Mathematics Journal International Journal of Educational Research Excellence (IJERE) Jurnal Sains dan Teknologi Farmasi Indonesia al Kimiya : Jurnal Ilmu Kimia dan Terapan ACTIVE: Journal of Physical Education, Sport, Health and Recreation
Toto Subroto
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang, 45363 Jawa Barat, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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The Effect of Single Co-expression of The DnaK-DnaJ-GrpE and GroEL/ES Chaperones and Their Combination on Expression Intein-pretrombin-2 in Escherichia coli ER2566 Iman Permana Maksum; D Agus Yusuf Wildan; Khomaini Hasan; Toto Subroto
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 6, No. 1, May 2020
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.174 KB) | DOI: 10.15408/jkv.v6i1.11333

Abstract

The use of recombinant thrombin in the manufacture of fibrin glue allows diseases contamination to be avoided. However, the expression of recombinant protein in E. coli still has a disadvantage of the formation of inclusion bodies, so it needs to be minimized by co-expression of chaperones. Therefore, the aim of this study was to determine the effect of single DnaK-DnaJ-GrpE and GroEL/ES chaperone expression and their combination on the expression of intein-pretrombin-2Ti,pH on E. coli ER2566. The method started with isolation of pTWIN1-prethrombin-2Ti,pH and pG-KJE8 from E. coli TOP10F' and DH5α respectively, the co-transformation of the expression host E. coli ER2566 using pG-KJE8 and pTWIN1-prethrombin-2Ti,pHvectors, the chaperone co-expression was induced using L-Arabinosa before IPTG induction and cell culture growth was incubated at 22 oC. The expression products were characterized by using Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The results of the co-expression of chaperone showed that the number of soluble fraction was higher than the one without co-expression of chaperone. In addition, the co-expression of chaperone using pG-KJE8 in intein-prothrombin-2Ti,pH expression was sufficient using tetracycline as an inducer.
Box-Behnken Experimental Design for Electrochemical Aptasensor Optimization on Screen Printed Carbon Electrode/Silica-Ceria Zakiyyah, Salma Nur; Eddy, Diana Rakhmawaty; Firdaus, Muhammad Lutfi; Subroto, Toto; Hartati, Yeni Wahyuni
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 1, May 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i1.27493

Abstract

This study aims to optimize the epithelial sodium channel (ENaC) electrochemical aptasensor with the Box-Behnken experimental design. ENaC is a protein that plays a role in sodium ion transport in several epithelial tissues and is associated with hypertension. The ENaC protein aptamer is held in place in the electrochemical aptasensor by a modified screen-printed carbon electrode (SPCE) of silica-ceria composite (SiO2-CeO2). The unique structure of a silica matrix with high biocompatibility can form composites through a hydrothermal process. The Box-Behnken (BBD) experimental design is an efficient optimization method of factors that affect the experiment at three levels. The FTIR results of the silica-ceria composites were 549.35 cm-1 (Ce-O), 1095.3 cm-1 (Si-O-Si), and 491.28 cm-1 (Si-O). Meanwhile, SPCE/silica-ceria characterized by differential pulse voltammetry (DPV) showed an increase in peak current [Fe(CN)6]3-/4- from 3.190 μA to 9.073 μA. Three experimental factors, aptamer concentration, streptavidin incubation time, and aptamer incubation time, were optimized with BBD and obtained at 0.5 μg.mL-1, 30 minutes, and 1 hour. The optimum conditions observed resulted in a selective current response for ENaC protein detection. The optimization results can be applied to aptamer-based ENaC protein detection in samples.
Co-Authors Abdul Alim Kahari, Abdul Alim Abu Bakar M.I. Syihab Agus Gumilar Alifa I. Nabila Amiruddin, Mohd. Hasril Anita Yuwita Anneke Noviyanti Aprillia, Mutiara Sukma Ari Hardianto Bachti Alisjahbana Basti Andriyoko Bisri Musthofa Budiantoro, Ogi D Agus Yusuf Wildan Dani Permana Debby M Sumanti Defi Supriyati, Ayu DESSY NATALIA DESSY NATALIA DESSY NATALIA Dewi Astriany Dhiya Salsabila Dian Budiana, Dian Diana P Rahmawati Didi Suryadi DWI RAHMAWATI Eddy, Diana Rakhmawaty Effendi, Syulastri EKO YULIANTO Elazmanawati Lembong Fadhilah, Muhammad Naufal Fadhlillah, Muhammad Fadillah, Lukky Farihandiandra, Yusron Fathurrohman, Muhammad Andi Fatin, Nuri Nuraini Fauzian Giansyah Febriani Aji Kusuma, Sherlynda FERNITA PUSPASARI Firdaus, Muhammad Lutfi Gifa Nur Arofah Gumelar, Abih Gumilar, Agus Hafizatunnisa, Hafizatunnisa Hambali, Burhan Hamdiyanti, Meti Henry Chandra Hesti Lina Wiraswati Idar Idar Idar Idar Idar Idar Idar, Idar Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum IMAN PERMANA MAKSUM IMAN PERMANA MAKSUM Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum Kharimah, Nurul Ikhsan KHOMAINI HASAN Kusumawardhani, Shinta Laelasari Laelasari Laelasari, Laelasari Leonardus Wiydatmoko Lesmana, Gianjar Mony, Siti Rasnawati Muchamad Subali Noto Muhammad Fadhil Z, Rusdi Noor Rosa (p:43-50) Muhammad Fadhlillah Muhammad Hilman Daniswara Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Murniaty Simorangkir Murniaty Simorangkir Naufal D, Kevin Nida Yumna Nisa Fauziah Noviyanti, Anneke Nurfairuz, Hasna Siti Nurmalasari, Ratna Nurul Ikhsan Karimah O Suprijana O Suprijana O Suprijana, O Ogi Budiantoro OGI BUDIANTORO Oo Suprijana Opik Taupiqurrohman Opik Taupiqurrohman Pertiwi, Wulan Praja, Ena Suhena Purba Upay Rahmaniar Mulyani Rahmat, Alit Riezki Amalia Rima Handiyani Rizky Rosjanuardi Rodiana, Ivta Safri Ishmayana Safri Ishmayana Safri Ishmayana Safrl Ismayana Sahroni, Sahroni Santhy Wyantuti Sari Agung, Syennie Sari Syahruni Sari Syahruni Saronom Silaban Saronom Silaban Saronom Silaban SARONOM SILABAN Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar -, Shabarni Gaffar SHABARNI GHAFFAR Shinta Kusumawardani Siti Soidah Siti Umayah, Siti Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro SOETIJOSO SOEMITRO Soetijoso Soemitro Sofyan Multazam N Aji Sriwidodo Sriwidodo Sriwidodo Sriwidodo, Sriwidodo Sriwidodo, . Sukma Nuswantara Sukma Nuswantara Sukma Nuswantara Sunan M. Alpiansyah Sutarya Enus Sutarya Enus SUTARYA ENUS Sutarya Enus Syani, Mamay Syfa Nurfadilah Taupiqurrohman, Opik Taupiqurrohman, Opik Tika Pradnjaparamita, Tika Tonah Triana N Meirina Umi Baroroh Unang Supratman Upay, Purba wahyu widayat WANGSA TIRTA ISMAYA Windy Aulia Putri Wulan Pertiwi Yani Suryani Yeni W Hartati Yeni Wahyuni Hartati Zakiyyah, Salma Nur