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EXOGENOUS APPLICATION OF TRYPTOPHAN AND INDOLE ACETIC ACID (IAA) TO INDUCE ROOT NODULE FORMATION AND INCREASE SOYBEAN YIELD IN ACID, NEUTRAL AND ALKALINE SOIL Sudadi, Sudadi; Suryono, Suryono
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The research aimed to study whether soil pH affects exogenous application of amino acid tryptophan and IAA in increasing root nodules and soybean yield. The experiment was conducted in greenhouse using 20 cm diameter plastic pot filled with fine soil, arranged in completely randomized design (CRD) with four treatments. Each treatment combination was repeated three times. Three seeds of local soybean variety were planted into each pot then to remain one plant a week after planting. Variables observed were root nodules number, shoot dry weight and seed yield. Plant nutrients were supplied in the form of solution to meet crop needs. The data obtained were analyzed statistically by F test at 5% of level confidence, followed by Duncans multiple range test when there was a significant effect. The results showed that both exogenous amino acid tryptophan and IAA increased number of root nodules, shoot and root dry weight and soybean yield. Higher root nodules number was taken from the treatment combination of 0.001 ppm IAA applied at V3 in Alfisol and Vertisols, and 1.0 ppm on Entisols. However, the highest soybean yields were taken from the treatment combination of 1.0 ppm tryptophan applied at V0 in Alfisols (6.51 g plant-1).Keywords: alfisol, entisol, extra cellular, phyto-hormones, vertisol 
Biosulfo Fertilizer Development for Horticulture Crops II. The Effect of Phosphate Rock Content and Inoculum Ratio of Biosulfo on P and S Uptake and Yield of Red Onion in Acid and Alkaline Soils Sudadi, Sudadi; Sumarno, Sumarno; Widada, Jaka
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Research aimed to study the influence of phosphate rock-sulfur (PRS) content and inoculum ratios of biosulfo on P and S uptake and red onion yield on acid (Alfisol) and alkaline (Vertisol) soils.  Two factors evaluated were PRS content (0%, 60%, 80%) and inoculums of A.niger/P.nalgiovensis ratio of biosulfo (0:0, 1:1 and 3:1). As much of 12 kg of soil (Ø 2 mm) mixed thoroughly with biosulfo, basic fertilizer and manure, put into polybag then watering at field capacity moisture content. One bulb of red onion was planted to each polybag and incubated in the green house. The experiments arranged in completely randomized design with three replications. Variables observed included P and S uptake, and onion yield. Data analyzed with F test at 5% level of significant followed with DMRT if any significant influences. The result shows that the increases of PRS content as well as A. niger ratio of biosulfo tend to increase P and S uptake, especially on acid Alfisol.   Highest P and S uptake and onion yield were achieved with treatment combinations of P80I11, P80I31, and P60 I11 for Alfisol,   and  P80 I11,  P60 I11 and P60 I11  for Vertisol respectively.   Keywords:  phosphate, rock content, inoculums ratio,P and S uptake, onion yield
EXOGENOUS APPLICATION OF TRYPTOPHAN AND INDOLE ACETIC ACID (IAA) TO INDUCE ROOT NODULE FORMATION AND INCREASE SOYBEAN YIELD IN ACID, NEUTRAL AND ALKALINE SOIL Sudadi, Sudadi; Suryono, Suryono
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i1.444

Abstract

The research aimed to study whether soil pH affects exogenous application of amino acid tryptophan and IAA in increasing root nodules and soybean yield. The experiment was conducted in greenhouse using 20 cm diameter plastic pot filled with fine soil, arranged in completely randomized design (CRD) with four treatments. Each treatment combination was repeated three times. Three seeds of local soybean variety were planted into each pot then to remain one plant a week after planting. Variables observed were root nodules number, shoot dry weight and seed yield. Plant nutrients were supplied in the form of solution to meet crop needs. The data obtained were analyzed statistically by F test at 5% of level confidence, followed by Duncan's multiple range test when there was a significant effect. The results showed that both exogenous amino acid tryptophan and IAA increased number of root nodules, shoot and root dry weight and soybean yield. Higher root nodules number was taken from the treatment combination of 0.001 ppm IAA applied at V3 in Alfisol and Vertisols, and 1.0 ppm on Entisols. However, the highest soybean yields were taken from the treatment combination of 1.0 ppm tryptophan applied at V0 in Alfisols (6.51 g plant-1). 
The Use of Biofilmed Biofertilizer to Improve Soil Chemical Fertility and Yield of Upland Kangkung (Ipomoea reptans) on Vertisol Sudadi, Sudadi; Putri, Ega Yuana; Suntoro, Suntoro
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 2 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.118.83-92

Abstract

The application of biofilm biofertilizer is potential to improve soil fertility and increase plant yield. The research aimed to assess the use of organic fertilizer decomposed with biofilm biofertilizer to improve soil fertility and yield of upland kale in Vertisol. The field experiment was conducted in Vertisol at Jaten, Karanganyar, Central Java, arranged in a Randomized Complete Block Design with a single factor, which was organic fertilizer dose consisting of 0, 3, 6, 9, 12, 15, 18, and 21 ton.ha-1 organic with NPK fertilizer as comparison treatment. Upland kale seeds were planted in 15 x 15 cm plant spacing. The variables observed were total nitrogen, available P, exchangeable K, soil organic matter, pH, cation exchange capacity, leaf number, plant height, fresh and dry weight. The data obtained were analyzed using F test followed by Duncan Multiple Range Test (DMRT) 95%. The result showed that the organic fertilizer dose had a significant effect on all of the observed variables. Optimal doses of organic fertilizer to improve soil fertility and upland kale yield was 15 - 18 ton.ha-1. The highest yield of upland kale was observed in the treatment of 21 ton.ha-1 organic fertilizer (76.5 ton.ha-1), which was increased by 176% compared to control (34.7 ton.ha-1) and by 108.8% (45.78 ton.ha-1) compared to NPK treatments. The application of 3 ton.ha-1 organic fertilizer gave better yield of upland kale than NPK fertilizer.
Pendugaan Kadar Biomassa dan Karbon Tersimpan pada Berbagai Kemiringan dan Tutupan Lahan di KHDTK Gunung Bromo UNS Tira Anggit Drupadi; Dwi Priyo Ariyanto; Sudadi Sudadi
Agrikultura Vol 32, No 2 (2021): Agustus, 2021
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v32i2.32344

Abstract

Perubahan tutupan lahan hutan mengakibatkan penurunan biomassa dan sekuestrasi karbon pada vegetasi. Penurunan biomassa dapat dipengaruhi faktor kemiringan lereng. Lereng yang curam diketahui menurunkan pertumbuhan vegetasi dan berdampak pada biomassa. Penelitian ini bertujuan untuk mengetahui pengaruh tutupan dan kemiringan lahan terhadap biomassa pohon, serasah dan karbon tersimpan serta mengetahui kadar biomassa pohon, serasah dan karbon tersimpan. Penelitian ini menggunakan metode survey dan penentuan titik pengamatan secara purposive sampling pada empat tutupan (Campuran, Mahoni, Pinus-Mahoni, Pinus) dan tiga kelas kemiringan lahan (0-15%, 15-30%, dan >30%). Estimasi biomassa menggunakan metode non-destructive dan dihitung menggunakan persamaan allometrik. Petak ukur dibuat berukuran 20 m x 20 m dengan jumlah 48 petak untuk pengamatan pohon dengan diameter ≥ 5 cm, kemudian dibuat sub petak untuk pengamatan serasah berukuran 1 m x 1 m. Hasil percobaan menunjukkan bahwa tutupan lahan Pinus menghasilkan biomassa pohon dan karbon tersimpan tertinggi bila dibandingkan dengan tutupan lahan lainnya dan variasi kemiringan menghasilkan biomassa pohon dan karbon tersimpan yang seragam atau sama. Kadar biomassa pohon dan karbon pada setiap tutupan lahan masing-masing berkisar antara 171,72–385 ton/ha dan 80,71–181,12 tonC/ha. Kadar biomassa serasah pada setiap tutupan berkisar antara 0,60–1,60 ton/ha/bulan.
Assessment of soil degradation in Pitu District, Ngawi Regency M Mujiyo; S Sumarno; S Sudadi; Retno Wisnu Murti
Journal of Degraded and Mining Lands Management Vol 7, No 2 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2020.072.2049

Abstract

This study aimed to determine the status of soil damage in Pitu District, Ngawi Regency. The study was conducted in a descriptive exploratory with survey methods. Determination of site sampling was done by purposive sampling, based on distribution or division and land use categories from the results of map overlays. Assessment of soil degradation potency was based on land units from the uniformity of the soil, rainfall, slope, and land use map. Land units resulting from overlay were scored based on the results of multiplication of weights and soil, slope, rainfall, and land use ratings. Total score indicates soil Degradation Potency (SDP). Assessment of soil degradation status was done through matching and scoring. Field observation results were matched with the standard criteria of soil degradation from Indonesian Government Regulation No. 150 of 2000. Each parameter was scored, then the total score was used to determine soil degradation status. Soil degradation potency in Pitu District, Ngawi Regency is PR II (Low) at LMU (Land Map Units) 1, 3, 4, 5, and 8, and PR III (Moderate) at LMU 2, 6, 7, 9, and 10. Soil degradation status in Pitu District, Ngawi Regency is slightly degraded at all LMU, with limiting factors, namely texture, bulk density, total porosity, and permeability.
The Use of Biofilmed Biofertilizer to Improve Soil Chemical Fertility and Yield of Upland Kangkung (Ipomoea reptans) on Vertisol Sudadi Sudadi; Ega Yuana Putri; Suntoro Suntoro
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 2 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.118.83-92

Abstract

The application of biofilm biofertilizer is potential to improve soil fertility and increase plant yield. The research aimed to assess the use of organic fertilizer decomposed with biofilm biofertilizer to improve soil fertility and yield of upland kale in Vertisol. The field experiment was conducted in Vertisol at Jaten, Karanganyar, Central Java, arranged in a Randomized Complete Block Design with a single factor, which was organic fertilizer dose consisting of 0, 3, 6, 9, 12, 15, 18, and 21 ton.ha-1 organic with NPK fertilizer as comparison treatment. Upland kale seeds were planted in 15 x 15 cm plant spacing. The variables observed were total nitrogen, available P, exchangeable K, soil organic matter, pH, cation exchange capacity, leaf number, plant height, fresh and dry weight. The data obtained were analyzed using F test followed by Duncan Multiple Range Test (DMRT) 95%. The result showed that the organic fertilizer dose had a significant effect on all of the observed variables. Optimal doses of organic fertilizer to improve soil fertility and upland kale yield was 15 - 18 ton.ha-1. The highest yield of upland kale was observed in the treatment of 21 ton.ha-1 organic fertilizer (76.5 ton.ha-1), which was increased by 176% compared to control (34.7 ton.ha-1) and by 108.8% (45.78 ton.ha-1) compared to NPK treatments. The application of 3 ton.ha-1 organic fertilizer gave better yield of upland kale than NPK fertilizer.
Capability of Sulphur Oxidizing Bacteria to Inhibit Basal Plate Rot and Increase Shallot Growth on Andisols Sudadi Sudadi; Hadiwiyono Hadiwiyono; Sumarno Sumarno; Dhani Dhyana Ciptasari
AGRIVITA, Journal of Agricultural Science Vol 41, No 1 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i1.829

Abstract

In the recent years, basal plate rot (BPR) caused by Fusarium oxysporum f. sp.cepae (FOCe) is one of the important constraints of shallot production in Indonesia. This research aimed to study the potential of S-oxidizing bacteria (SOB) as biological control agents to inhibit FOCe and provide available-S to shallot on Andisols. An experiment in laboratory was conducted to evaluate the capabilty of SOB in oxidizing S in liquid medium and a pot experiment was aimed to evaluate the capability of SOB in providing available-S and inhibiting infection of shallot by FOCe in Andisols. All treatments were arranged by a Completely Randomized Design with three replications. The data were analyzed by F test, followed by Duncan’s Test.The results showed that the capability of oxidizing S in a liquid medium increased after the incubation for six days, with the highest concentration of soluble-S was taken from SOB2 isolate. The isolate provided the highest available-S, while the highest capability of decreasing the disease incidence was taken by SOB3 and SOB1+2+3. SOB3 isolates promoted the highest growth of shallot. So it has a tremendous potential if they are used as an inoculum of bio-fertilizer and biological control agent of BPR of shallot.
Biosulfo Fertilizer Development for Horticulture Crops II. The Effect of Phosphate Rock Content and Inoculum Ratio of Biosulfo on P and S Uptake and Yield of Red Onion in Acid and Alkaline Soils Sudadi Sudadi; Sumarno Sumarno; Jaka Widada
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.83

Abstract

Research aimed to study the influence of phosphate rock-sulfur (PRS) content and inoculum ratios of biosulfo on P and S uptake and red onion yield on acid (Alfisol) and alkaline (Vertisol) soils.  Two factors evaluated were PRS content (0%, 60%, 80%) and inoculums of A.niger/P.nalgiovensis ratio of biosulfo (0:0, 1:1 and 3:1). As much of 12 kg of soil (Ø 2 mm) mixed thoroughly with biosulfo, basic fertilizer and manure, put into polybag then watering at field capacity moisture content. One bulb of red onion was planted to each polybag and incubated in the green house. The experiments arranged in completely randomized design with three replications. Variables observed included P and S uptake, and onion yield. Data analyzed with F test at 5% level of significant followed with DMRT if any significant influences. The result shows that the increases of PRS content as well as A. niger ratio of biosulfo tend to increase P and S uptake, especially on acid Alfisol.   Highest P and S uptake and onion yield were achieved with treatment combinations of P80I11, P80I31, and P60 I11 for Alfisol,   and  P80 I11,  P60 I11 and P60 I11  for Vertisol respectively.   Keywords:  phosphate, rock content, inoculums ratio,P and S uptake, onion yield
The Use of Agrobacterium sp.I3 and Compost as Chelator Combined by NPK 3 Fertilizer and Mendong Plant (Fimbristylis sp.) in Bioremediation of Paddy Soil Contaminated by Lead (Pb) RETNO ROSARIASTUTI; ABDI LEONARDO SARAGIH; SUDADI SUDADI; SUPRIYADI SUPRIYADI; WIWIN WIDIASTUTI
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1888.536 KB) | DOI: 10.5454/mi.13.3.1

Abstract

Industrial waste supplies contains heavy metals such as Pb which will cause pollution in paddy fields. Remediation of paddy soil contaminated by Pb heavy metal must be done by simple, environmental friendly, cheap and sustainable technology, that is bioremediation. The purpose of this study was to study the effectiveness of bioremediation using Agrobacterium sp. I3 and compost as chelator combined by Mendong plant and NPK fertilizer, and learn the ability of Mendong in uptaking metal soil Pb. This was field experimental research, had a factorial patern, using Completly Randomized Block Design as the base design, with three factors: (1) NPK fertilizers (P0: no NPK fertilizers, P1: with NPK fertilizers), (2) Chelator (K0: no chelator; K1: with chelator Agrobacterium sp. I3 ; K2: with chelator compost); and (3) Plant (T0: without plant; T1: with Mendong plant). The results showed that Agrobacterium sp. I3 and compost were increasing Pb uptake in shoot, but decreasing Pb uptake in root. Mendong plant has highly ability in uptaking soil Pb, so decreased soil Pb, and effective as the phytoremediator. NPK fertilizer increased plant growth so increased Pb uptaken by plant. The highest Pb uptake was in treatment combination of NPK fertilizer + Mendong plant: 80.916 µg, followed by NPK fertilizer + Agrobacterium sp. I3 + Mendong Plant: 76.363 µg. The highest decreased of soil Pb (42.41%) was found in treatment combination of compost + Mendong Plant. Key words: Agrobacterium sp.I3, compost, Fimbristylis sp, Pb, phytoremediation
Co-Authors -Ridani, Ahmad . Hadiwiyono Abda Abda ABDI LEONARDO SARAGIH Achmad Ruslan Afendi Agung Nugroho, Fauzi Ahmad Ridani Alatas, Muhyi Majid Alivia, Tiara Anak Agung Gede Sugianthara Anandeya Satrio Sambodo, Anandeya Satrio Anwar Anwar Aprilianti, Yuni Arbain Arbain, Arbain Arkam Lahiya Aryo Andri Nugroho Ashlihati, Diena Bahrani Bambang Suryono, Bambang Bayu Rahmad Bernadip Bowo Adiyanto Canggih Jati Nusantara, Canggih Jati Chairul Anwar Claudia Sandy Sofani Demelia Arida Ariyanti Dhani Dhyana Ciptasari Djayanti Sari Dwi Priyo Ariyanto Edi Purwanto Ega Yuana Putri Eka Mahmud, Muhammad Eko Eri Sambodo Endang Setyorini Erwin Purniawati Etty Nurbayani, Etty Hadiwiyono Hadiwiyono Hadiwiyono Hadiwiyono Hadiwiyono Hadiwiyono Haetami, Haetami Hamdi, Liwaul Haniati, Isna Luthfa Hapsari, Estima Titi Harditia, Harditia Harhara, Andre Aggasy Hery Widijanto Hifni, Hifni Hikmah K, Nur Adilah Raudatul Ika Ernawati Iwan Fuadi Jaka Suyana JAKA WIDADA Jarkawi Jarkawi Jazmi, Kamarul Azmi Joko Sulianto . . Khojir Kundharu Saddhono Kurniawati, Juni Linda Habsari Efendi Putri Loveana, Okta Lutfy Ismoyo Madjid, Mohammad Fikri Halim Mahmud Mahmud Mangastuti, Rebecca Sidhapramudita Maro'ah, Siti Martaria, Nency MM Rudi Prihatno, MM Rudi Mohammad Denny Permana Muadin, Akhmad Muchammad Eka Mahmud, Muchammad Eka Muhammad Taufiqurrahman, Muhammad Muhsin Muhsin Mujiyo Mujiyo Novitasari, Rizki Cahya Nugroho, Ragil Catur Nunik Iriyanti Ramadhan Nur Sabilla, Widya Putri Oetoro, Bambang J. Ongko Cahyono Parwati Parwati Poromarto, Susilo Hamberg Pramono, Sidik Purwanto Purwanto Purwanto Purwanto Putri, Ega Yuana Rachmawatie, Srie Juli Radifan Gustisiya, Muhammad Ryan Rahmatisa, Dimas Rahmatisa, Dimas Ramadhina, Marini Shadrina Ramdhan, Tri Wahyudi Ramli, Akhmad Ramli, H. Akhmad Rani Rahmawati Ratih Kirana Ratih Kumala Fajar Apsari Retno Rosariastuti Retno Wisnu Murti Rina Andriani Rizka Novi Sesanti Rosita Rosita S Sumarno Samanhudi Samanhudi Selly Maisyarah Shofiyah, Laily Simanjuntak, Alvina Siswahyudianto Siti Julaiha Slamet Minardi Sri Hartati Sri Rahardjo Suharyatun, Suharyatun Sumarno . Sumarno Sumarno Sumarno Sumarno Sumarno Sumarno Sumarno Sumarno Sumarno Sumarno Suntoro Suntoro Suntoro Suntoro Suntoro Suntoro Supriyadi Supriyadi Surono Surono Suryono Suryono Suryono Suryono Sutami, Sutami Suyasa, Agus Baratha Suyasa, Agus Baratha Taufiqqurahman, Muhammad Tira Anggit Drupadi Trijayanti, Christiana Vita Ratri Cahyani Vivit Nurcahyani Widiyanto, Teguh Heru Widyatmani Sih Dewi Wijaya, Lidya Zaela Wiji Hastuti, Wiji Wiki Handi Wiwin Widiastuti Yasinta Choirina Yusnia Binti Kholifah Zainal, Muhammad Zuhair