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Sukenda, Sukenda,
Department Of Aquaculture, Faculty Of Fisheries And Marine Science, Bogor Agricultural University, Bogor
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Distribution of Vibrio parahaemolyticus in pacific white shrimp Litopenaeus vannamei through immersion as a natural infection model Sari Anggraeni, Sukma; Sukenda; Nuryati, Sri; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.176-188

Abstract

Vibrio parahaemolyticus is a pathogenic bacterium that indusces mortality is pacific white shrimp. This study aimed to analyze the distribution of V. parahaeomyticus in shrimp through immersion and evaluate, polymerase chain reaction, histopathology, and immune response. The study employed two treatments with four replicates each. Shrimp were infected through immersion with V. parahaemolyticus at a concentration of 107 CFU/mL for 30 minutes, followed by transferring the shrimp to a rearing container. Gill, hepatopancreas, gut, and hemolymph samples were collected at 6, 12, and 24 hours post-infection. Infected shrimp exhibited clinical symptoms such as a pale body and hepatopancreas, along with empty gut. Shrimp mortality reached 97.08%, while no mortality occurred in the control grouph. The abundance of V. parahaemolyticus in organs exceeded 105 CFU/mL. Histopathological examination revealed mild, moderate, and severe damage. PCR confirmation tests at 12 and 24 hours yielded positive results, with a DNA band at 230 bp. Immune responses, including total hemocytes, phagocytic activity, phenoloxidase, and respiratory burst, demonstrated significantly different results (P<0.05). It was concluded that the distribution of V. parahaemolyticus in the shrimp body, specifically in the gills, hepatopancreas, and gut, exceed 105 CFU/mL, as confirmed by a 230 bp DNA band in the PCR test. Tissue damage, manifested as lesions and necrosis, occurred in the gills, hepatopancreas, and gut. Additionally, the imun response of Vibrio-infected shrimp was found to be lower than that of uninfected shrimp (negative control). Keywords: distribution, immersion, Litopenaeus vannamei, Vibrio parahaemolyticus ABSTRAK Vibrio parahaemolyticus adalah bakteri patogen yang menyebabkan kematian pada udang vaname. Penelitian ini bertujuan menganalisis distribusi V. parahaemolyticus pada udang melalui perendaman serta kerusakan yang ditimbulkan melalui pengamatan gejala klinis, angka lempeng total bakteri, polymerase chain reaction, histopatologi dan respons imun. Penelitian menggunakan dua perlakuan dan empat ulangan. Udang diinfeksi melalui perendaman menggunakan V. parahaemolyticus 107 CFU/mL selama 30 menit, kemudian udang dipindah pada wadah pemeliharaan. Pengambilan organ insang, hepatopankreas, usus dan hemolim dilakukan pada jam ke-6, 12, dan 24 pascainfeksi. Udang terinfeksi memiliki gejala klinis seperti tubuh dan hepatopankreas pucat, usus kosong. Mortalitas udang mencapai 97.08% dan tidak ada kematian pada udang kontrol. Kelimpahan V. parahaemolyticus pada organ lebih dari 105 CFU/mL. Pengamatan histopatologi menunjukkan kerusakan ringan, sedang, hingga parah. Uji konfirmasi PCR jam ke-12 dan ke-24 menunjukkan hasil positif pada 230 bp. Respons imun seperti total hemosit, aktivitas fagositik, phenoloxidase, respiratory burst menujukkan hasil yang berbeda nyata (P<0.05). Disimpulkan bahwa distribusi V. parahaemolyticus di dalam tubuh udang pada terdapat pada insang, hepatopankreas dan usus lebih dari 105 CFU/mL dan terkonfirmasi uji PCR pada pita DNA 230 bp. Kerusakan jaringan seperti lesi dan nekrosis terjadi pada insang, hepatopankreas, dan usus. Respons imun udang yang terinfeksi Vibrio lebih rendah dibandingkan udang yang tidak terinfeksi (kontrol negatif). Kata kunci: distribusi, Litopenaeus vannamei, perendaman, Vibrio parahaemolyticus
Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health Iqbal Kurniawinata, Mohamad; Sukenda, Sukenda; Wahjuningrum, Dinamella; Widanarni, Widanarni; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.71-81

Abstract

This study evaluated the efficacy of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum in improving the immune response and resistance of whiteleg shrimp to prevent Vibrio parahaemolyticus infection. The study consisted of two phases, an in vitro phase to determine the compounds contained in three medicinal plants as antibacterials, followed by an in vivo phase to evaluate the effect of the medical plant extract on immune response and robustness against V. parahaemolyticus. The results from the first phase revealed that bioactive compounds present in E. longifolia were more varied and had higher concentrations with a lower bactericidal value when compared to those found in C. zedoaria or A. sativum. In the second phase of the experiment, the medicinal plant extract was added to the feed with a dose that was determined according to the first phase results. The treatments tested in the second phase were 1.6% E. longifolia extract dietary addition (EL16), 6.4% C. zedoaria extract dietary addition (CZ64), 6.4% A. sativum extract dietary addition (AS64) and phytobiotics mixture of 1:1:1 (C1) dietary addition, as well as no phytobiotic for negative control treatment and positive control. The results from the second stage demonstrated that dietary phytobiotic extract addition enhances the immunological responses and improves the shrimp survival against V. parahaemolyticus challenge compared to the control group. In conclusion, E. longifolia, C. zedoaria, and A. sativum showed different bioactive compound profiles, which affect their efficacy against V. parahaemolyticus, with EL16 showing higher efficacy. Keywords: A. sativum, C. zedoaria, E. longifolia, Penaeus vannamei, Phytobiotic ABSTRAK Penelitian ini mengevaluasi efikasi Eurycoma longifolia, Curcuma zedoaria, dan Allium sativum dalam meningkatkan respon imun dan resistensi udang vaname untuk mencegah infeksi Vibrio parahaemolyticus. Penelitian ini terdiri dari dua tahap: tahap in vitro untuk menentukan senyawa yang terkandung dalam tiga tanaman obat sebagai antibakteri, diikuti oleh tahap in vivo untuk mengevaluasi efek ekstrak tanaman obat terhadap respon imun dan ketahanan terhadap V. parahaemolyticus. Hasil dari tahap pertama mengungkapkan bahwa senyawa bioaktif yang ada dalam E. longifolia lebih bervariasi dan memiliki konsentrasi yang lebih tinggi dengan nilai bakterisida yang lebih rendah jika dibandingkan dengan yang ditemukan di C. zedoaria atau A. sativum. Pada percobaan tahap kedua, ekstrak tanaman obat ditambahkan ke pakan dengan dosis yang ditentukan sesuai dengan hasil tahap pertama. Perlakuan yang diuji pada tahap kedua adalah penambahan 1,6% ekstrak E. longifolia dalam pakan (EL16), penambahan 6,4% ekstrak C. zedoaria dalam pakan (CZ64), penambahan 6,4% ekstrak A. sativum dalam pakan (AS64) dan campuran fitobiotik 1:1:1 (C1) dalam pakan, serta tanpa fitobiotik untuk perlakuan kontrol negatif dan kontrol positif. Hasil dari tahap kedua menunjukkan bahwa penambahan ekstrak fitobiotik dalam pakan meningkatkan respons imunologi dan memperbaiki kelangsungan hidup udang terhadap tantangan V. parahaemolyticus dibandingkan dengan kelompok kontrol. Sebagai kesimpulan, E. longifolia, C. zedoaria, dan A. sativum menunjukkan profil senyawa bioaktif yang berbeda, yang mempengaruhi efikasinya terhadap V. parahaemolyticus, dengan EL16 menunjukkan efikasi yang lebih tinggi. Kata kunci: Allium sativum, Curcuma zedoaria, Eurycoma. longifolia, fitobiotik, Penaeus vannamei
Isolation of Lytic Bacteriophages infected Indonesian-strain Vibrio parahaemolyticus and its Protective Effects on Brine Shrimp (Artemia sp.) Wahjuningrum, Dinamella; Ramhirez, Putri Shandra; Nuzullia, Laely; Yuhana, Munti; Sukenda, Sukenda; Nasrullah, Hasan
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 1 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.67419

Abstract

Graphical Abstract Highlight Research vB_Vp_TSK01 and vB_Vp_JKT02 a specific parahaemolyticus bacteriophage were isolated from the shrimp aquaculture water. Compared to control vB_Vp_TSK01 reduced 11.46±1.35% of bacterial growth, and by 9.86±5.92% for vB_Vp_JKT02 treatment. Their cocktail had the highest parahemolyticus growth inhibition by 30.92±3.89%. The phage treatment increased the survival of the Artemia by 28.57% compared to infection control.     Abstract Acute Hepatopancreatic Necrosis Disease (AHPND) caused by V. parahaemolyticus infection was one of the major diseases in shrimp culture in recent years. The Vibrio could also affect the survival of Artemia as the shrimp’s main live feed in the hatchery and they become the possible carrier for the AHPND. Phage therapy in shrimp aquaculture could reduce the application of the antibiotic as an antibacterial agent for the AHPND. The present study aimed to isolate the specific lytic phage for the Indonesian strain of V. parahaemolyticus (Vp) and evaluate the phage therapy for the brine shrimp Artemia infected with the Vp. The Vp-specific phage was isolated from the shrimp farm’s water at Tasikmalaya, and North Jakarta City, Indonesia. After isolation and plaque assay, brine shrimp were used as a model to evaluate the phages’ anti-Vibrio activity The Vp-lytic phage was successfully isolated from shrimp culture water at North Jakarta and Tasikmalaya (Vb_Vp_TSK01 and Vb_Vp_JKT01, respectively) and the results showed that both isolated phages and their cocktails were capable to inhibit the growth of Vp with the highest inhibition shown at the cocktail treatment (p<0.05). The survival of Artemia was higher in the phage treatments (p<0.005) compared to the infected control. Infected control had 68.33% of brine shrimp survival, and the Vb_Vp_TSK01, Vb_Vp_JKT01, and their cocktail had similar average brine shrimp survival of 91.11%. In conclusion, phage therapy proved effective in preventing vibriosis in brine shrimp under the conditions tested.
APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (Litopenaeus vannamei) (Synbiotic Application for Prevention of Infectious Myonecrosis Virus Infection in White Shrimp (Litopenaeus vannamei)) Widanarni, Widanarni; Sukenda, Sukenda; Septiani, Ghita Ryan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5041

Abstract

This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.4930.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.930.025-6.970.019% and had higher values than controls (A and B) (P0.05). Meanwhile, feed conversion value in C and D (1.540.142 and 1.580.117) were lower than controls (P0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.
EVALUASI SISTEM PRODUKSI UDANG VANAME Litopenaeus vannamei SAAT TERJADI WABAH PENYAKIT IMNV DI TAMBAK UDANG PINANG GADING, BAKAUHENI, LAMPUNG SELATAN Hidayatullah, Dendi; Fadlilah, Rizqy Aditya; Sukenda, Sukenda; Effendi, Irzal
OCTOPUS: JURNAL ILMU PERIKANAN Vol. 13 No. 1 (2024): OCTOPUS
Publisher : Universitas Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26618/o.v13i1.15027

Abstract

Udang vaname (Litopennaeus vannamei) merupakan salah satu komoditas unggulan budidaya perairan di Indonesia. Penyakit udang merupakan salah satu tantangan yang dihadapi oleh pembudidaya. Salah satu penyakit yang menyerang udang adalah infectious myonecrosis (IMN). Tujuan penelitian ini adalah mengevaluasi sistem produksi budidaya udang vaname saat terjadi wabah infeksi infectious myonecrosis virus IMNV di Tambak Pinang Gading, Bakauheni, Lampung Selatan. Sebanyak 2 petak tambak udang digunakan sebagai petak pengamatan yaitu petak A7 (2700 m2) dan A8 (3700 m2) dengan padata tebar udang masing-masing 74 ekor/m2 dan 54 m2. Kegiatan budidaya udang dilakukan mulai dari persiapan hingga panen. Penelitian dilakukan dengan mengevaluasi kinerja produksi dan kelayakan usaha budidaya udang vaname. Hasil penelitian menunjukkan bahwa  budidaya udang pada kondisi baik, kelangsungan hidup udang dapat mencapai 94,75% dengan keuntungan Rp. 43.335.000/tahun dan kondisi tidak baik saat terserang IMNV mencapai 34,83% dengan kerugian Rp. 49.906.080/tahun. Oleh karena itu, dapat disimpulkan bahwa budidaya udang vaname memiliki peluang usaha yang baik namun juga memiliki resiko kerugian akibat penyakit seperti IMNV. Evaluasi standar operasional prosedur (SOP) dan peningkatan biosekutitas perlu dilakukan untuk menjaga agar produksi budidaya udang tetap baik dan berkelanjutan.
ADMINISTRATION OF Curcuma spp. EXTRACT TO CONTROL Aeromonas hydrophila INFECTION IN STRIPED CATFISH (Pangasianodon hypophthalmus) Sasanti, Ade Dwi; Widanarni, Widanarni; Sukenda, Sukenda; Yuhana, Munti; Wahjuningrum, Dinamella; Setiawati, Mia
Indonesian Aquaculture Journal Vol 20, No 1 (2025): (June, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.1.2025.23-36

Abstract

Curcuma spp. is a phytobiotic with potential application in fish farming. This study aimed to evaluate the potential of Curcuma zedoaria, Curcuma aeruginosa and Curcuma mangga extracts in striped catfish infected with Aeromonas hydrophila. The study used a complete randomized design (CRD) consisting of six treatments: KN (negative control), KP (positive control, fish infected without treatment), PE (C. zedoaria extract 6.25 g kg-1), HE (C. aeruginosa extract 6.25 g kg-1), ME (C. mangga extract 6.25 g kg-1) and KE (combination of C. zedoaria extract 2.1 g kg-1 + C. aeruginosa extract 2.1 g kg-1 + C. mangga extract 2.1 g kg-1). A total of 360 striped catfish, 10 ± 0.5 cm were kept in 18 aquariums measuring 50 × 40 × 35 cm3 and fed the treatment for 30 days. The challenge test was conducted on day 31 by injecting A. hydrophila suspension (106 colony-forming units (CFU) mL-1) intramuscularly into fish. The results showed that the treatment administered were able to stimulate the expression of interleukin-1â, interferon-ã 2a, 2b genes, increase the number of red and white blood cells, hematocrit, hemoglobin, phagocytosis activity, respiratory burst, reduce the population of A. hydrophila in the intestine, and reduce tissue damage in striped catfish. Striped catfish treated with the extracts showed significantly higher survival rates (p<0.05) compared to the positive control group. The survival rates were: KN (100.00 %), KP (53.33%), PE (93.33 %), HE (91.67 %), ME (93.33 %), and KE (88.33 %). In conclusion, the treatment administered were able to enhance the immune response and resistance of striped catfish infected with A. hydrophila.
EFFECT OF NEGATIVE REDOX POTENTIAL OF DRY SEDIMENT ON THE INFECTIVITY OF Vibrio parahaemolyticus, GROWTH AND HEALTH STATUS OF PACIFIC WHITELEG SHRIMP Putri, Shofii Amaliah; Sukenda, Sukenda; Yuhana, Munti; Nirmala, Kukuh
Indonesian Aquaculture Journal Vol 20, No 2 (2025): (December, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.2.2025.133-144

Abstract

Sediment serves as a site for the accumulation of aquaculture waste. Oxidation-Reduction Potential (ORP) is an index indicating anaerobic conditions. A combination of host, pathogen, and suboptimal environmental quality can lead to disease outbreaks. One such disease is Acute Hepatopancreatic Necrosis Disease (AHPND), caused by Vibrio parahaemolyticus. This study was aimed to analyse the impact of sediment drying on infectivity of V. parahaemolyticus growth and health status of Pacific whiteleg shrimp. The sediment ORP used at the initial study was -37 mV. The sediment was dried for 120 hours at a temperature of 40.75 ± 2.73 °C. A total of 15 shrimp/aquarium with an average body weight of 2.35±0.22 g/individual were reared in aquarium sized 20x25x30 cm3 containing dried and undried sediment for 120 hours, then contaminated with 105 CFU/mL V. parahaemolyticus through immersion from the start of rearing until 20 days later. The results showed that sediment drying was able to reduce bacterial abundance and increase ORP sediment. The longer duration of cultivation, the more the sediment ORP decreased. Sediment ORP decreased with increasing shrimp farming duration. The highest bacterial abundance in the sediment on thrid phase of study was observed in the positive control treatment on D+10 ((2.02±0.39)x104 CFU/g), then it decreased until the end. Meanwhile, bacterial abundance in water and hepatopancreas decreased until the end of the study. Survival rates, immune responses, and growth parameters of shrimp with sediment drying were higher than the positive control (p<0.05) but not significantly different from the negative control. The histopathology of shrimp hepatopancreas profile in the sediment drying treatment was better compared to the positive control.
RESPONS IMUN UDANG VANAME PADA SISTEM BIOFLOK DENGAN KEPADATAN BERBEDA TERHADAP INFEKSI Vibrio parahaemolyticus Widanarni, Widanarni; Fadilah, Iin Nur; Cahyawati, Nadia; Yuhana, Munti; Sukenda, Sukenda; Gustilatov, Muhamad
Media Akuakultur Vol 20, No 2 (2025): Desember 2025
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.20.2.2025.51-61

Abstract

Budidaya udang vaname (Litopenaeus vannamei) pada kepadatan tinggi meningkatkan stres dan risiko infeksi bakteri, khususnya Vibrio parahaemolyticus. Teknologi bioflok (BFT) melalui penambahan karbon  organik dapat meningkatkan biomassa mikroba heterotrof yang memperbaiki respons imun dan menurunkan virulensi bakteri. Penelitian ini bertujuan menganalisis respons imun dan resistansi udang yang dipelihara dengan padat tebar berbeda dalam sistem bioflok terhadap infeksi V. parahaemolyticus. Udang (0,44±0,02 g) dipelihara selama 7 hari sebanyak 16 perlakuan hasil kombinasi sistem bioflok dan kontrol (tanpa bioflok) dengan kepadatan 4, 8, 12, dan 16 ekor L-¹, lalu diuji tantang melalui perendaman dengan dosis V. parahaemolyticus 10u CFU mL-¹ dimulai sejak awal perlakuan. Hasil menunjukkan bahwa sistem bioflok meningkatkan parameter imun udang, termasuk total hemocyte count (THC), aktivitas fagositik (AF), respiratory burst (RB), dan aktivitas phenoloxidase (PO) secara signifikan (P<0,05) dibandingkan kontrol. Kelimpahan V. parahaemolyticus di air dan tubuh udang perlakuan bioflok lebih rendah dan berbeda signifikan (P<0,05) dengan kontrol positif. Bioflok juga meningkatkan tingkat kelangsungan hidup, dengan nilai pada kepadatan 4 ekor L-¹ (83,33±5,77%), dan tetap lebih baik dibandingkan kontrol hingga kepadatan 16 ekor L-¹. Sistem bioflok terbukti efektif meningkatkan respons imun, menekan infeksi V. parahaemolyticus, dan mempertahankan kelangsungan hidup udang vaname pada kepadatan tinggi.
Kebijakan Inovatif untuk Akuakultur Berkelanjutan: Optimalisasi Peran Bivalvia dalam Pengolahan Limbah Eva Prasetiyono Pras; Kukuh Nirmala; Eddy Supriyono; Sukenda; Yuni Puji Hastuti
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol. 7 No. 2 (2025): Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0702.1272-1276

Abstract

Pertumbuhan industri tambak udang intensif di Indonesia telah memberikan kontribusi ekonomi yang signifikan. Namun tambak udang juga menimbulkan permasalahan lingkungan akibat buangan kaya nutrien seperti amonium, nitrat, ortofosfat, partikel organik dan tersuspensi. Kandungan nutrien yang tinggi dalam buangan tambak dapat memicu ledakan populasi mikroalga, menurunkan kualitas lingkungan dan terganggunya keseimbangan ekosistem perairan. Pemerintah telah mengeluarkan kebijakan yang mewajibkan setiap tambak udang memiliki unit pengolahan limbah sebagai bentuk pengendalian dampak lingkungan. Namun, implementasi kebijakan ini menghadapi tantangan besar terkait dengan besarnya biaya investasi dan operasional instalasi pengolahan limbah tersebut. Upaya untuk memberikan solusi alternatif yang lebih efisien dan ramah lingkungan harus dilakukan, diantaranya dengan menggunakan bivalvia. Pemanfaatan bivalvia— seperti kerang darah—dapat menjadi pendekatan biotreatment yang efektif. Bivalvia berperan sebagai filter feeder yang mampu mengasimilasi mikroalga dan partikel nutrien di dalam air. Filtrasi menggunakan kerang darah terbukti mampu menurunkan konsentrasi mikroalga dan nutrien, masing-masing sebesar 72,80% untuk mikroalga, 36,78% untuk amonia, 50,00% untuk nitrit, 78,77% untuk nitrat, dan 89,13% untuk ortofosfat.. Rekomendasi alternatif kebijakan diarahkan pada integrasi penggunaan bivalvia dalam sistem pengolahan limbah tambak, dan penyusunan pedoman teknis biotreatment yang lebih efisien sebagai bagian dari strategi pengelolaan limbah berkelanjutan. Strategi ini tidak hanya memperkuat upaya perlindungan lingkungan, tetapi juga membuka peluang ekonomi tambahan melalui produksi bivalvia sebagai komoditas konsumsi.
POTENCY OF VIBRIO ISOLATES FOR BIOCONTROL OF VIBRIOSIS IN TIGER SHRIMP (PENAEUS MONODON) LARVAE WIDANARNI WIDANARNI; A. SUWANTO; SUKENDA SUKENDA; B. W. LAY
BIOTROPIA No. 20 (2003)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.682 KB) | DOI: 10.11598/btb.2003.0.20.180

Abstract

This study was carried out to obtain Vibrio isolates able to function as biocontrol of vibriosis in shrimp hatchery. Thirty one Vibrio isolates were isolated from tiger shrimp larvae and hatchery environments, i.e. Labuan, Pangandaran, and Lampung, Indonesia. Pathogenic  V. harveyi MR5339 was obtained from Maros, South-Sulawesi and was made as a rifampicin resistant mutant (RFR) to screen for those 31 Vibrio isolates in in vitro assays and to allow us to monitor their presence in shrimp larvae and larval rearing water. Almost all Vibrio  isolates could inhibit the growth of pathogenic V. harveyi MR5339 RFR. SKT-b isolate from Skeletonema was the most effective to inhibit the growth of V. harveyi MR5339 Rf* and significantly reduced larval mortality in pathogen challenge assays. These prospective biocontrol bacteria, at concentration of 10" CFU/ml, did not show pathogenicity to shrimp larvae. SKT-b was Gram negative, short rod-shape, exhibited yellow colonies on TCBS and swarming on SWC-agar media, motile, utilized glucose and sucrose but not lactose: produced extra-cellular protease and amylase, but did not produce chitmase. Partial sequencing of 16S-rRNA gene SKT-b showed SKT-b similarity to Vibrio alginofyticus. Keywords: shrimp larvae / biocontrol bacteria / vibriosis.
Co-Authors , Rahman, , , Ranta, , , Rusli, , . ARIFUDDIN . Maryani . Sunarto A. Hasan A. Santika A. SUWANTO A. Suwanto A.J. Sihombing ADE DWI SASANTI Ade Dwi Sasanti Aditya, Tiya Widi Afif, Usamah Afiff , Usamah Agung Cahyo Setyawan Ahmad Beni Rouf Aldy Mulyadin Alimuddin Alimuddin Alimuddin Alit Brilliant Aminatul Zahra Amrullah Amrullah Andi Parenrengi Andi Parenrengi Andi Tenriulo Andi Tenriulo Angela M Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Nugrahawati Annisa Astri Anggraeni Ardana Kurniaji Ayi Santika Azis Azis B. W. LAY Bambang Gunadi Bambang Gunadi Brite, Margie Cahyawati, Nadia Catur A. Pebrianto D. Dana D. Meha D. Wahjuningrum Daniel Djokosetiyanto Daniel Djokosetiyanto Daniel Djokosetiyanto DEBY YUNIASARI Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah, Dendi Desy Sugiani Desy Sugiani Dewi Rahmi DIAH AYU SATYARI UTAMI Dian Febriani Dinamella Wahjuningrum Dwi Agung Saputra E. Ayuzar E. Harris Eddy Supriyono Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja Enang Harris Surawidjaja Endang Susianingsih Endang Susianingsih Esti Handayani Hardi Eva Prasetiyono Eva Prasetiyono Pras F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadilah, Iin Nur Fadlilah, Rizqy Aditya Fahmi Rajab Firmansyah, Arif Lukman Fitria Novianti Ghita Ryan Septiani Gustilatov, Muhamad Henky Manoppo Henky Manoppo Hisatsugu Wakabayashi Huda Salahudin Darusman Huda Shalahudin Darusman I Wayan Nurjaya I. Normalina I. Nur I. Tepu Ibnu Bangkit Bioshina Suryadi Ince Ayu Khairana Kadriah Ince Ayu Khairani Kadriah Inem Ode Iqbal Kurniawinata, Mohamad Irzal Effendi Isni Rahmatika Sari Jasmanindar, Yudiana Jean-Christophe Avarre Jeanni Indah Noermala Julie Ekasari K. Sumantadinata Khairun Nissa Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kukuh Nirmala L. Jamal Lastriliah, Mira M. Yuhana M. Zairin Junior M.S. Arifin MIA SETIAWATI Mia Setiawati Mochamad Fatuchri Sukadi Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi MUHAMMAD AGUS SUPRAYUDI Muhammad Yamin Muhammad Zairin Jr. Muhammad Zairin Jr. Muharram Nur Ikhsan Mulyani, Rahma MUNTI YUHANA N.A. Maswan Nasri Julaini Nasrullah, Hasan Nunak Nafiqoh Nunuk Listiyowati Nur Bambang Priyo Utomo Nurbariah Nurbariah Nuzullia, Laely Odang Carman Ode, Inem P. Hadi Pratiwi, Kiki Amalia Priadi Setyawan Putri, Shofii Amaliah R.D. Soejoedono Rafsyanzani, Muhammad Mufthi Rahma Mulyani Rahman Rahman Ramadhani, Dian Eka Ramhirez, Putri Shandra Retno Damayanti Soejoedono RIDWAN AFFANDI Ririn Nurul Fauziah, Ririn Nurul Rizki Praseto, Rizki Ronald Kriston Sauttua Nainggolan Rr. Bellya Anasti Maharani Rudi, Mad Ruku Ratu Borut Samsu Adi Rahman Sari Anggraeni, Sukma Sefti Heza Dwinanti, Sefti Heza Septiani, Ghita Ryan Siti Subaidah Sri Nuryati Sri Nuryati Sugeng Budiharsono Sugiyo Hadi Pranoto Suhermanto, Achmad Tatag Budiardi Tatik Mufidah Tatik Mufidah Trian Rizky Febriansyah Tsani Untsa, Agista Tuti Sumiati Tuti Sumiati Wesly Pasaribu Wida Lesmanawati Widanarni WIDANARNI WIDANARNI WIDANARNI WIDANARNI WIDANARNI WIDANARNI Wisriati Lasima Wiyoto Wiyoto Woro Nur Endang Sariati Y. Hadiroseyani Y. Tri Anggoro Yan Evan Yosmaniar Yosmaniar Yosmaniar Yosmaniar Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zulfani, Anisa