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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Penelitian Saintek Agrikultura Zuriat Journal of Tropical Soils Jurnal Fakultas Pertanian JURNAL AGROTEKNOLOGI Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Agro Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Kultivasi AGRISAINTIFIKA Jurnal Ilmu-ilmu Pertanian JURNAL GALUNG TROPIKA Jurnal Pengabdian Pada Masyarakat Jurnal Biologi Tropis Agrin : Jurnal Penelitian Pertanian MATAPPA: Jurnal Pengabdian Kepada Masyarakat Jurnal Agrotek Tropika Martabe : Jurnal Pengabdian Kepada Masyarakat J-Dinamika: Jurnal Pengabdian Kepada Masyarakat AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Jurnal ABDINUS : Jurnal Pengabdian Nusantara Jurnal Agrotropika JAGO TOLIS : Jurnal Agrokompleks Tolis Jurnal Agroteknologi Jurnal Ilmiah Kesehatan Delima Jurnal Kajian Budaya dan Humaniora (JKBH) Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat Jurnal Kesehatan Tujuh Belas (Jurkes TB)
Erni Suminar
Universitas Padjadjaran
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Journal : Jurnal Agro

 1 
Pengujian berbagai eksplan kentang (Solanum tuberosum L.) dengan penggunaan konsentrasi BAP dan NAA yang berbeda Fitri Widya Lestari; Erni Suminar; Syariful Mubarok
Jurnal Agro Vol 5, No 1 (2018)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/1348

Abstract

Rendahnya produksi kentang di Indonesia disebabkan oleh ketersediaan benih kentang bermutu hasil dari metode konvensional yang kurang memadai. Metode kultur jaringan mampu menghasilkan bibit bermutu dan bebas virus, dalam jumlah banyak serta waktu yang singkat. Penelitian ini bertujuan untuk mendapatkan konsentrasi 6-benzylaminopurine (BAP) dan Naphthalene Acetic Acid (NAA) terbaik pada berbagai eksplan untuk pertumbuhan tunas meriklon kentang (Solanum tuberosum L.) Kultivar Atlantik secara in vitro. Percobaan dilaksanakan pada November 2016 sampai Februari 2017 di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Rancangan Acak Lengkap (RAL) digunakan sebagai rancangan percobaan yang terdiri dari dua unit percobaan terpisah yaitu unit eksplan meristem interkalar dan meristem apikal, masing-masing delapan perlakuan dan empat ulangan. Media Murashige and Skoog (MS) digunakan sebagai media dasar dengan penambahan BAP (0,0 mg L-1; 1 mg L-1; 1,5 mg L-1; 2 mg L-1) dan NAA (0,0 mg L-1; 0,5 mg L-1). Hasil menunjukkan konsentrasi BAP 1 mg L-1 merupakan perlakuan yang paling baik dalam menghasilkan jumlah tunas, cabang, daun dan buku pada eksplan meristem interkalar. Pada eksplan meristem apikal, penambahan BAP dan NAA belum mampu meningkatkan pertumbuhan eksplan. Meristem interkalar yang diberi auksin dan sitokinin berpotensi menjadi bahan alternatif eksplan meriklon kentang selain meristem apikal. The production of potato in Indonesia is low due to the limited number of high quality potato seeds produced from the conventional methods. The tissue culture methods can be used to produce high quality and virus-free seeds in more reliable number in a short time. Randomized Completely Design (RCD) was used as the experimental design consisted of two separate trial units namely intercalary meristem and apical meristem explants units with eight treatments and four replications of each. Murashige and Skoog (MS) was used as the base media added with BAP i.e. 0.0 mg L-1; 1 mg L-1; 1.5 mg L-1; 2 mg L-1 and NAA 0.0 mg L-1 and 0.5 mg L-1. Results showed that concentration of BAP 1 mg L-1 generated the best results on the number of shoots, branches, leaves and nodes on meristem intercalary explant. Meanwhile on apical meristem explant, the addition of BAP and NAA had not yet improved the explant growth. Intercalary meristem added with auxin and cytokinin is potential as alternative of mericlone explants material of potato besides the apical meristem.
Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro Lamro Purba; Erni Suminar; Denny Sobardini; Wieny Rizky; Syariful Mubarok
Jurnal Agro Vol 4, No 2 (2017)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/1344

Abstract

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable.
Co-Authors A.A. Ketut Agung Cahyawan W Ade Ismail Ade Ismail Ade Ismail Ade Ismail Ade Setiawan Afgani, Chairul Anam Agung Rahmadi Agung Rahmadi Agus Purwito Alin Robiah Al Adawiyah Alvianto, Muhamad Amalia, Inneke ANNE NURAINI Anne Nurani ANNE NURBAITY Anni Yuniarti Arin Rosmala Ariyanto, Nur Budi Ayu Ratna Ningrum Ayuwardani, Novi Azizah, Annisa Nanda Nur Bambang Nurhadi Bella Dian Ratnasari. Bella Dian Ratnasari., Bella Dian Camelia Andriani Citra Bakti, Citra Denny Sobardini Denny Sobardini Denny Sobardini Denny Sobardini Sobarna Denny Sobardini Sobarna Denny Sobardini, Denny Dian Ardiansyah Dian Ardiansyah Dikdik Kurnia Dilla Nitra Gustiana Dinnur Afiifah Dinnur Afiifah, Dinnur Dirga Sapta Sara Dwi Hastuti Edi Pramono Ega Raisya Ega Raisya Elma, Tiara Elok Zubaidah Endah Yulia Erizon, Meisyela Salsabila Eva Aprilia Fadhillah, Maya Farida Damayanti Farida Farida Fauzia Khaerunnisa Millenia Fitri Widya Fitriatin Fitri Widya Lestari Fitrianti Widya Lestari G. G. Pitaloka G. G. Pitaloka, G. G. Ganjar Herdiansyah Gina Gustiani Pitaloka Gumilar, Roni H. Apriyanto H. Apriyanto, H. Hapizhah Hapizhah Hapizhah Hapizhah, Hapizhah Hiroshi Ezura Hiroshi Ezura Huda, Fahmi Alamil Inneke Amalia Intan Ratna Dewi Anjarsari Iwan Setiawan Jajang Sauman Hamdani Jihan Nabilla Kusumadewi, Vira Kusumiyati Lamro Purba Lindung Tri Puspasari Marhan Nurullia Maritha, Vevi Masako Akutsu Meddy Rachmadi Meisyela Erizon Mia Munggarani Mira Ariyanti Mita Indriani Mohammad Zulkarnain Muhamad Alvianto Muhamad Kadapi Muhamad kadapi, Muhamad Muhammad Abdillah Hasan Qonit Muhardiono, Iman Murgayanti Murgayanti Mustika Arsri Na’im, Aidi Nia Agustina Nita Suswati Endah Rini Noladhi Wicaksana Noor Istifadah Novi Ayuwardani Nur Azizah Romadhoni Nur Budi Ariyanto Nursuhud Nursuhud Pamungkas, Barolym Tri Paramita Wisnuwardhani, Paramita Pujawati Suryatmana Qonit, Muhammad Abdilah Hasan Rahmad, Sukardi Sugeng Rahmat Budiarto Rahmawati, Vira Raisya, Ega Rani Andriani Budi Kusumo Reginawanti Hindersah Rifkarosita Putri Ginaris Rija Sudirja Rika Meliansyah Rina Nurmaulawati Roni Gumilar Roni Gumilar Rosniawati, Santi Rubaekah, Siti Sarah Rufaidah, Fathi S Sumadi Santi Rosniawaty Santika Sari Sasmita, Heny SIska Rasiska, SIska Siti Julaeha, Siti Siti Rakhmah Tenrisui Pakki Siti Rakhmah Tenrisui Pakki Siti Sarah Rubaekah Sobir Sobir Sri Hartati Suci Azima, Nuzula Sudarjat Sudarjat Sudarjat Sudarjat Sudarjat Sulistyaningsih Sulistyaningsih Sulistyaningsih Sulistyaningsih Sumadi Sumadi Sumadi Sumadi SUMADI SUMADI Sumadi Sumadi Sumadi Sumadi SYARIFUL MUBAROK Tiara Elma Toto Sunarto Trisnawati, Desi Ucu Wandi Somantri Venny Revia Viola Vevi Maritha Vijaya Isnaniawardhani Wawan Sutari Wieny Heriliya Rizky Wieny Heriliya Rizky, Wieny Heriliya Wieny Marma Jaya Wieny Rizky Wiharti, Nabila Ragil Y. Sholihin Y. Sholihin, Y. Yayat Rochayat Suradinata Yenny, Ratna Fitry Yudianto, Tri Yudithia Maxiselly, Yudithia Yulianto, Fiky