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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Prosiding Seminar Biologi bionature Techno.Com: Jurnal Teknologi Informasi Proceeding International Conference on Global Resource Conservation Natural B Journal of Tropical Biodiversity and Biotechnology THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Biosaintifika: Journal of Biology & Biology Education Martabe : Jurnal Pengabdian Kepada Masyarakat Berdikari: Jurnal Pengabdian Masyarakat Indonesia Bioedukasi: Jurnal Pendidikan Biologi Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) GERVASI: Jurnal Pengabdian kepada Masyarakat Jurnal Karinov Abdi Kami : Jurnal Pengabdian Kepada Masyarakat Jurnal Graha Pengabdian Jurnal Pengabdian UNDIKMA Jurnal Pengabdian Masyarakat Indonesia Proceeding Biology Education Conference Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Sci-Tech Journal Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Jurnal Masyarakat Madani Indonesia Jurnal MIPA dan Pembelajarannya Jurnal Inovasi Teknologi dan Edukasi Teknik VISA: Journal of Vision and Ideas Symbiotic: Journal of Biological Education and Science Prosiding Seminar Nasional Unimus Jurnal Ilmu Hayat Journal of Innovation and Teacher Professionalism ABDI KAMI: Jurnal Pengabdian Kepada Masyarakat
Agung Witjoro
Universitas Negeri Malang
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Kualitas Nata de Apple dengan Perbedaan Varietas Apel (Malus sylvestris Mill) dan Macam Gula Berdasarkan Tebal, Berat dan Kadar Serat Rofiqoh Lailatul Fitriyah; Utami Sri Hastuti; Agung Witjoro
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

Apple has the most fruit productio at Batu City. There are two apple  varieties; Manalagi cherry and Anna, but some apple varieties have low quality (grade D). The reason is because of low price and the consumers is not interesting to buy, so grade D apple decreased. An alternative to apply the grade D apple is by using for nata production with addition three sugar sort, that is cane sugar, palm sugar and siwalan sugar as a food diversification. This study aims to (1) examine the effect of varieties of apples on the quality of nata based on the weight, thickness and fiber content, (2) to examine the effect of three kinds of sugar on the quality of nata based on the weight, thickness and fiber content , (3) to analyze the difference effect of apples varieties and sugars sort that produce the highest quality nata. The results showed that (1) the apples varieties has significant effect on the nata quality based on the nata fiber content, but there is no significant effect of apples varieties on the nata quality based on the thickness and weight of nata. varieties Manalagi cherry variety produces the best nata quality based on the fiber content, (2) The sugar sorts significantly affect to the nata quality based on thickness, weight and nata fiber content. The use of palm sugar produces the best nata quality based on the thickness, weight and fiber content, (3) The interaction of apple varieties and sugars sorts significantly affect to the nata quality based on the thickness, weight and nata fiber content. Manalagi cherry variety with the addition of palm sugar gives the best nata quality based on the thickness, weight and fiber content
ANALISIS MIKROBIOLOGI MINUMAN TEH SEDUHAN BERBEDA MERK BERDASARKAN NILAI MPN COLIFORM DI KOTA MALANG Ana Syarifatun Nisa; Utami Sri Hastuti; Agung Witjoro
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 9, No 1 (2012): Prosiding Seminar Nasional IX Biologi
Publisher : Universitas Sebelas Maret

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Abstract

ABSTRAK   Minuman teh seduhan memiliki beberapa kelebihan dalam menarik minat konsumen, diantaranya kemasan yang mudah dibawa dan selalu segar tetapi juga dapat mengalami kontaminasi oleh bakteri. Penelitian ini bertujuan untuk mengetahui 1) Nilai MPN coliform dan coliform fekal minuman teh seduhan yang dijual di tiga pusat perbelanjaan Kota Malang; 2) Kualitas mikrobiologi minuman teh seduhan yang dijual di tiga tempat pusat perbelanjaan Kota Malang. Jenis penelitian ini ialah penelitian deskriptif observasional. Penelitian dilaksanakan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA UM pada bulan Maret-Mei 2012. Sampel yang digunakan adalah minuman teh seduhan rasa original merk A dan B yang dijual dari tiga pusat perbelanjaan Kota Malang. Pengambilan sampel dari tiap penjual diambil sebanyak tiga kali, dengan interval waktu dua minggu. Data penelitian hasil perhitungan nilai MPN coliform dan coliform fekal, dirujukkan pada nilai standar MPN coliform maksimum BPOM RI, Nomor HK.00.06.1.52.4011 tahun 2009. Hasil penelitian menunjukkan: 1) Nilai MPN coliform pada sampel minuman teh seduhan merk A dan B yang dijual di tiga tempat pusat perbelanjaan Kota Malang secara berutut-turut ialah >2400 sel/ml dan >2400 sel/mL; 2) Nilai MPN coliform fekal pada sampel minuman teh seduhan merk A dan B yang dijual di tiga tempat pusat perbelanjaan Kota Malang secara berturut-turut ialah >2400 sel/ml dan >2400 sel/mL. 3) Nilai MPN coliform dan coliform fekal minuman teh seduhan kedua merk melebihi standar yang telah ditetapkan oleh BPOM RI yaitu < 3 sel/mL. Ditinjau dari nilai MPN coliform dan nilai coliform fekal dapat disimpulkan bahwa kualitas mikrobiologi minuman teh seduhan merk A dan B dari tiga tempat pusat perbelanjaan Kota Malang kurang memenuhi syarat kelayakan konsumsi.   Kata kunci: minuman teh seduhan, nilai MPN coliform, nilai MPN coliform fekal.
Improving Community Skills in Making Batik Jumputan Through Training PKK Mothers at Sumbergempol, Kabupaten Tulungagung Siti Imroatul Maslikah; Murni Saptasari; Sitoresmi Prabaningtyas; Srirahayu Lestari; Agung Witjoro; Mimien Henie Irawati; Susriyati Mahanal; Dyah Ayu Eka Fitriana; Muhammad Athoillah; Yuslinda Anisa; Siti Nur Arifah; Alif Rofiqotun Nurul Alimah
Berdikari: Jurnal Pengabdian Masyarakat Indonesia Vol. 2 No. 1 (2019): Berdikari: jurnal Pengabdian Masyarakat Indonesia
Publisher : Future Science

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Abstract

The purpose of this study is to improve the community’ skills of making batik jumputan through training of 21 PKK mothers at Sumbergempol District, Tulungagung Regency. This type of research is descriptive qualitative. Participants involved in this study were selected randomly (random sampling technique). Data collection techniques in the form of skills test making batik jumputan (paper based-test) conducted before and after training. Data analysis was performed using the calculation and categorization of N-Gain. The results showed that the training in making batik jumputan could improve the skills of PKK mothers at Sumbergempol District, Tulungagung Regency in the moderate (62%) and high (38%) categories.
Identifikasi dan Enumerasi Escherichia coli dengan Kombinasi Metode MPN-PCR Atik Kurniawati; Dwi Listyorini; Agung Witjoro
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.7592

Abstract

Healthy and nutritious food should be free from biological contaminants. The presence of Escherichia coli in food is an indicator contamination and also presence of other pathogenic bacteria. This study aims to determine the value of MPN E. coli in food samples using the MPN-PCR combination technique. Descriptive research method. Samples were taken from 5 traders in Malang City. The results showed that 3 out of 5 food samples were contaminated with E. coli, indicated by the presence of a specific band amplified and produced a DNA band measuring 584 base pairs in agarose gel electrophoresis. Conclusion: MPN value of E. coli in the three food samples code II 34 MPN / gram, code III 75 MPN / gram and V code 290 MPN / gram. The MPN-PCR combination can be used as an alternative method for the conventional MPN test because it can detect the presence of E. coli bacteria in food quickly and reliably
The Role of Black Soybean and Purple Sweet Potato Active Compound on Advanced Glycation End-Product in Streptozotocin-Induced Type 2 Diabetes Mellitus Rat Abdul Gofur; Siti Nur Arifah; Yuslinda Annisa; Agung Witjoro; Mochammad Fitri Atho'illah; Sri Rahayu Lestari
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.02

Abstract

Diabetic nephropathy is one of the diabetes complications attacking kidney leading to kidney damage. Hyperglycemia accompanying DM causes the increase of Ad-vanced Glycation End-Product (AGE) and Receptor Advanced Glycation End-Product (RAGE) activity, then develop kidney damage and other diabetes compli-cations. The study aimed to investigate the effect of black soybean, purple sweet potato, or their combination on the expression of AGE, RAGE, and kidney necro-sis T2DM model rats. The rats were given with high-calorie diet for five weeks and then injected with a low dose of streptozotocin (30 m/kg Body Weight) in intraperitoneal. DM rats were divided into: normal, K- (T2DM), K+ (T2DM + glibenclamide 0.6 mg/kg body weight), P1 (T2DM + black soybean), P2 (T2DM + purple sweet potato), and P3-5 Combination 1-3 (T2DM + combination of black soybean and purple sweet potato in ratio of 1 : 3, 2 : 2, and 3 : 1). DM rats were then given the treatments for thirty days. The effect of black soybean, purple sweet potato, or the combination of both was evaluated through the expression of AGE, RAGE, and necrosis of renal tubules. The changes in renal tubules histological characteristics were evaluated using hematoxylin-eosin (HE) staining. Immuno-histochemistry analysis of renal tubules was to evaluate AGE-RAGE expression after the treatments. The research results indicated that there was a significant dif-ference from the combination of black soybean and purple sweet potato in reduc-ing AGE, RAGE, and renal tubules necrosis. The BSB and PSP combination ratio of 1:1 was able to improve renal tubules, decrease the expression of AGE and RAGE towards near normal. The combination of black soybean and purple sweet potato could be used as one of the alternatives to improve kidney damage in dia-betic nephropathy.
Kualitas Nata de Apple dengan Perbedaan Varietas Apel (Malus sylvestris Mill) dan Macam Gula Berdasarkan Tebal, Berat dan Kadar Serat Rofiqoh Lailatul Fitriyah; Utami Sri Hastuti; Agung Witjoro
Bioedukasi: Jurnal Pendidikan Biologi Vol 12, No 1 (2019): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.734 KB) | DOI: 10.20961/bioedukasi-uns.v12i1.27408

Abstract

Apple has the most fruit productio at Batu City. There are two apple  varieties; Manalagi cherry and Anna, but some apple varieties have low quality (grade D). The reason is because of low price and the consumers is not interesting to buy, so grade D apple decreased. An alternative to apply the grade D apple is by using for nata production with addition three sugar sort, that is cane sugar, palm sugar and siwalan sugar as a food diversification. This study aims to (1) examine the effect of varieties of apples on the quality of nata based on the weight, thickness and fiber content, (2) to examine the effect of three kinds of sugar on the quality of nata based on the weight, thickness and fiber content , (3) to analyze the difference effect of apples varieties and sugars sort that produce the highest quality nata. The results showed that (1) the apples varieties has significant effect on the nata quality based on the nata fiber content, but there is no significant effect of apples varieties on the nata quality based on the thickness and weight of nata. varieties Manalagi cherry variety produces the best nata quality based on the fiber content, (2) The sugar sorts significantly affect to the nata quality based on thickness, weight and nata fiber content. The use of palm sugar produces the best nata quality based on the thickness, weight and fiber content, (3) The interaction of apple varieties and sugars sorts significantly affect to the nata quality based on the thickness, weight and nata fiber content. Manalagi cherry variety with the addition of palm sugar gives the best nata quality based on the thickness, weight and fiber content
Single Clove Garlic (Allium sativum) Essential Oil as an Inhibitor of Staphylococcus aureus Bacteria Abdul Gofur; Ida Wulandari; M. Fitri Athoillah; Agung Witjoro; Sri Rahayu Lestari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.13944

Abstract

Staphylococcus aureus is gram-positive bacteria that often infect the skin. S. aureus has been experiencing resistance to several antibiotics. One of the solutions to overcome the resistance is by using garlic that is believed by the society can overcome bacterial infection. The study aimed to find out the influence of the variation in concentration of local single clove garlic essential oil used on the inhibition and damage of morphological structure of S. aureus bacteria. The inhibition test was conducted using disc diffusion method. The experiment groups consisted of  1% DMSO as negative control, vancomycin 30µg/ml as positive control and single clove garlic essential oil (25 mg/ml, 50 mg/ml, 75 mg/ml and100 mg/ml) as treatment groups. The diameter of inhibition zone was measured using calipers. The morphological damage of the bacterial cells can be seen using Scanning Electron Microscopy (SEM) with magnification of 25000x. The result of Kruskal-Wallis test analysis indicated that the extract of local garlic essential oil has inhibitory activities against S. aureus bacteria (P0.05). The damage to the morphological structure of bacterial cells with the administration of 30 µg/ml vancomycin was equal to 100 mg/ml single clove garlic essential oil extract. Single clove garlic essential oil can be used as an alternative treatment for skin infection diseases by inhibiting S. aureus growth.
Peningkatan Nilai Tambah Buah Lokal di Desa Bangunsari melalui Pendampingan dan Pelatihan dalam Pengembangan Kue Buah Agung Witjoro; Ajeng Daniarsih; Hendra Susanto; Zahra Firdaus; Alby Aruna; Adinda Marcelliantika; M Iqbal Najib Fahmi; Eka Putri Surya
Prosiding Seminar Nasional Unimus Vol 6 (2023): Membangun Tatanan Sosial di Era Revolusi Industri 4.0 dalam Menunjang Pencapaian Susta
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tujuan dari kegiatan pengabdian masyarakat ini untuk meningkatkan nilai tambah produk buah lokaldengan memberikan pelatihan praktis tentang cara mengolah buah menjadi kue buah. Kegiatan ini berhasilmeningkatkan pengetahuan dan keterampilan masyarakat lokal dalam mengolah buah-buahan lokal yangunik melalui pudding buah dengan pendekatan berbasis Asset Based Comunnity Development (ABCD).Hasil evaluasi menunjukkan peningkatan ekonomi masyarakat melalui peningkatan pendapatan daripenjualan kue buah. Kegiatan ini juga membantu menjaga keanekaragaman hayati buah lokal dan membukapeluang untuk pasar kue buah lokal yang lebih besar melalui produk unggulan dan partisipasi aktif petanibuah lokal Desa Bangunsari. Kata Kunci: Pemanfaatan, Nilai Tambah, Buah, Desa Bangunsari
Pendampingan Gaya Hidup Sehat Guru Taman Kanak-Kanak Laboratorium Universitas Negeri Malang Melalui Plant Base Meat Agung Witjoro; Hendra Susanto; Ajeng Daniarsih; Zahra Firdaus; Alby Aruna; M Iqbal Najib Fahmi; Jasmine Nurul Izza
GERVASI: Jurnal Pengabdian kepada Masyarakat Vol. 7 No. 3 (2023): GERVASI: Jurnal Pengabdian kepada Masyarakat
Publisher : LPPM IKIP PGRI Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31571/gervasi.v7i3.6601

Abstract

Program pengabdian masyarakat (PkM) ini bertujuan untuk meningkatkan pengetahuan dan kesadaran tentang gizi dan mempromosikan pilihan makanan sehat di kalangan guru taman kanak-kanak Laboratorium Universitas Negeri Malang. Program ini fokus pada pendekatan inovatif dan berkelanjutan serta mempromosikan daging berbasis tumbuhan sebagai alternatif yang lebih sehat. Kegiatan PkM ini dilakukan melalui kolaborasi antara tim pelaksana dan mitra, mencakup lokakarya memasak, sesi tanya jawab, dan evaluasi. Data dikumpulkan melalui survei analisis kebutuhan, yang menilai tingkat pengetahuan awal partisipan tentang gizi dan preferensi makanan yang dilakukan di TK Laboratorium Universitas Negeri Malang. Setelah pelatihan, daging berbasis tumbuhan diolah dalam sesi memasak, dan evaluasi pasca-program dilakukan untuk mengukur efektivitas pendekatan ini. Hasil menunjukkan peningkatan signifikan dalam pengetahuan tentang gizi dan minat untuk mencoba makanan berbasis tumbuhan. Selain itu, peserta juga mengalami perubahan positif dalam memilih pola makan sehari-hari yang lebih sehat seperti konsumsi sayur, buah, dan protein. Program ini berhasil membuka wawasan guru-guru terhadap pilihan makanan yang lebih sehat dan berkelanjutan.
Efektivitas Ekstrak Minyak Atsiri Bawang Tunggal terhadap Bakteri Pseudomonas aeruginosa berdasarkan Profil Scanning Elektron Mikroskop Soeyati Poejiani; Sri Rahayu Lestari; Agung Witjoro
Jurnal Ilmu Hayat Vol 2, No 1 (2018)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um061v2i12018p21-33

Abstract

Infeksi nosokomial yang disebabkan oleh bakteri Pseudomonas aeruginosa menjadi sulit dalam terapi pengobatan, hal ini disebabkan karena penularan infeksi yang begitu cepat dan sifat resistensinya terhadap antibiotik, sehingga untuk mengatasi hal tersebut diperlukan suatu pengembangan pengobatan alternatif yang berkhasiat sebagai antibakteri yang berasal dari bahan alami yaitu tumbuh-tumbuhan salah satunya adalah bawang tunggal. Bawang tunggal mengandung senyawa organosulfur salah satunya adalah minyak atsiri yang di dalamnya terdapat allisin, alliin dan ajoene (Lestari dan Rifai., 2017). Penelitian ini bertujuan untuk mengukur daya hambat ekstrak minyak atsiri bawang tunggal terhadap pertumbuhan bakteri Pseudomonas aeruginosa secara in vitro dengan metode difusi cakaram dan kerusakan bakteri diamati berdasar profil scanning elektron mikroskop. Konsentrasi ekstrak minyak atsiri yang digunakan yaitu 100mg/ml, 75mg/ml, 50mg/ml, 25mg/ml, Ceftazidime 30µg/ml. Hasil uji ANAVA tunggal diketahui (p < 0,05) yang menunjukkan adanya nilai yang signifikan pada masing-masing perlakuan ekstrak minyak atsiri bawang tunggal dalam menghambat pertumbuhan bakteri Pseudomonas aeruginosa. Kerusakan bakteri diamati dengan Scanning Elektron Mikroskop menunjukkan bakteri mengalami pengerutan, sel menipis dan lisis. Simpulan penelitian yaitu ekstrak minyak atsiri bawang tunggal dapat menghambat pertumbuhan bakteri Pseudomonas aeruginosa dengan kriteria kuat yaitu pada konsentrasi 100mg/ml.
Co-Authors Abdul Gofur Abdul Gofur Abdul Gofur Achmad Hamdan Addakhil, Muhammad Fazlur Rahman Adinda Marcelliantika Agung Wibowo Ahmad Afa Haadiyan Wiyadi Ahmad Taufiq Ainun Nikmah Aisyah, Dahniar Nur Ajeng Daniarsih Al Ihsan, Rifqi Alby Aruna Alif Rofiqotun Nurul Alimah Ana Syarifatun Nisa Anastya Eka Kharisma Anggun Rakhmawati, Tri Annisa Avrilia Annisa, Yuslinda Argeshwara, Dityo Kreshna Arindra Nirbaya Aripriharta - Arrohman, Maulana Ludfi Aruna, Alby Aryansyah Pratama Atho'illah, Mochammad Fitri Athoillah, M. Fitri Atik Kurniawati Aulia Koen Syarafibi Avrilia, Annisa Bagus Priambodo Baroroh, Alif Rosyidah El Choirunnisa, Hesti Nur Desy Rachmawati Dewi, Putri Elok Septiana Dwi Agustin Angelina Dwi Listyorini Dyah Ayu Eka Fitriana Eka Putri Surya Fatchur Rohman Frida Kunti Setiowati Fuad Indra Kusuma Galang Raka Abdilah Hadi Suwono Hendra Susanto Hesti Nur Choirunnisa Humairah Rizky Nopiyanti I Made Wirawan I Wayan Sumberartha Ida Wulandari Izza, Jasmine Nurul Jasmine Nurul Izza Kusmayadi, Claresia Kusmayadi, Claresia Tsany Lelitawati, Mardiana Lely Hermawati M Iqbal Najib Fahmi M. Alfian Mizar M. Fitri Athoillah Mahmud Iskandar Mariya Sulfa, Devi Miasih, Dewi Sekar Mimien Henie Irawati Mochammad Fitri Atho'illah Mokh Sholihul Hadi Muhammad Andry Prio Utomo Muhammad Athoillah Murni Sapta Sari Muslihati Muzaqi, Safrial Ahmad Nopiyanti, Humairah Rizky Nurul Andini Setiawan Nurul Izza, Jasmine Nuurul Muchlishiin Oktavia, Silvi Tri Pratama, Aryansyah Primilestari, Lilik Rahma, Amalia Nur Ria Yustika Sari Rifqi Al Ihsan Rinata, Viska Roedy, Ocha Rofiqoh Lailatul Fitriyah Rudianto, Rony Sa'diyah, Halimatus Sari, Ria Yustika Shidiqi, Maulana As Siti Imroatul Maslikah Siti Nur Arifah Siti Nur Arifah Siti Nur Arifah, Siti Nur Sitoresmi Prabaningtyas Soeyati Poejiani Soeyati Poejiani Sofia Ery Rahayu Sri Rahayu Lestari Stefanus Nahas Subadra, ST. Ulfawanti Intan Suci Ramadhani Suci Ramadhani, Suci Sugiono, Bhima Satria Rizky Sujito Sujito Sulisetijono Sulisetijono Sumarli Sumarli, Sumarli Sunaryono Sunaryono Sunaryono, Sunaryono Suprayitno Suprayitno Susriati Mahanal Susriyati Mahanal Thoriq Bachtiar Y. E Tri Anggun Rakhmawati Umi Fitriyati Utami Sri Hastuti Wahyu Pratama, Ade Wulandari, Ida Yheni Sapitri Yunita Rakhmawati Yuslinda Anisa Yuslinda Annisa Zahra Anggita Pratiwi Zahra Firdaus Zulkifli, Muhammad Mauludi