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Journal : Ovozoa: Journal of Animal Reproduction

Mulberry (Morus alba L.) leaf extract enhanced spermatozoa motility, viability, and plasma membrane integrity of rats (Rattus norvegicus) exposed to e-cigarette smoke Ramadhani, Fifi Fauziah; Yulianna Puspitasari; Hermin Ratnani; Budi Utomo; Rochmah Kurnijasanti; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.120-128

Abstract

This study investigates the impact of mulberry leaf extract on the viability, plasma membrane integrity, and motility of spermatozoa from male white rats (Rattus norvegicus) exposed to e-cigarette smoke. A total of twenty-five male rats were divided into five groups: negative control (NC), positive control (PC), T1, T2, and T3. All groups, except for the NC group, were exposed to e-cigarette smoke. Rats in the T1, T2, and T3 groups received mulberry leaf extract in doses of 100, 200, and 400 mg/kg bw, respectively, while the NC and PC groups were given a placebo of 1% Na-CMC. Both the mulberry leaf extract and the placebo were administered daily, beginning three days prior to the start of e-cigarette smoke exposure, which lasted for 28 days. Results showed that spermatozoa motility, plasma membrane integrity, and viability in the experimental groups were significantly lower than those in the NC group (p <0.05). Conversely, rats in the T1, T2, and T3 groups that received mulberry leaf extract demonstrated significantly greater spermatozoa viability, plasma membrane integrity, and motility compared to the PC group (p <0.05). The T3 group exhibited the most pronounced improvements, with significantly enhanced spermatozoa viability, membrane integrity, and motility (p <0.05) relative to the PC group. These results indicate that mulberry (Morus alba L.) leaf extract enhanced spermatozoa viability, plasma membrane integrity, and motility in white rats (Rattus norvegicus) subjected to e-cigarette smoke.
Risk factors of pyometra in cat at several clinics in Kediri, East Java, Indonesia Rosyta, Pegy; Rimayanti Rimayanti; Budi Utomo; Jola Rahmahani; Yulianna Puspitasari; Wiwik Misaco Yuniarti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.115-119

Abstract

Pyometra is a reproductive disease that most often affects cats which is caused by bacterial infection of the uterus. This study aims to determine the risk factors that could influence the occurrence of pyometra in cats. The study was conducted at the Four Leg Pet Care, Asa Animal Clinic, and K-5 Animal Care in Kediri regency in December 2021 to February 2022. This retrospective cohort study took secondary data for the period 2020 to 2021 as research material and found 35 cats diagnosed with pyometra. The odds ratio was calculated to analyze the associations to some factors including age, body weight, use of hormonal contraception, frequency of administration of hormonal contraception, hormonal contraceptive drugs used, dose of hormonal contraception given.  Analysis of the relationship between risk factors for pyometra showed that the factors age, use of hormonal contraception, and frequency of giving hormonal contraceptives each have p values ​​of 2.17, 2.13, and 2.13, respectively. It could be concluded that age, use of hormonal contraception, and frequency of administration of hormonal contraception influenced or had an association with the risk of pyometra in cats.
Co-Authors Aan Nurfahrudianto Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Afandi, Zainal Agus Muji Santoso Aswin, Ahmad Bea Hana Siswati BUDI UTOMO Budiarto Budiarto Budiarto Bura, Maria Antonia Yersi Dua Dadik Rahardjo, Dadik Delaiah, Diena Diyantoro Diyantoro, Diyantoro Dua Bura, Maria Antonia Yersi Elis Irmayanti Endang Sri Mujiwati Endang Suprihati Ericka Darmawan Guruh Sukma Hanggara Hani Plumeriastuti Helen Susilowati, Helen Hermin Ratnani Heru Budiono Hidanah, Sri Hisyam, Mirza Atikah Madarina Jola Rahmahani Jola Rahmahani Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Khalissa Farah Alifia Kinasih, Kurnia Nisa Kirana, Sabila Citra Kuncorojakti, Suryo Kurniawan, Muhammad 'Ahdi Kusnoto Kusnoto, Kusnoto Larasati, Anissa Dhea Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Maulana, Firdausy Kurnia Mohamad Amin Moses, Ikechukwu Benjamin Mufasirin Mustika, Yolla Rona Mustofa Helmi Effendi Nidom, Chairul Anwar Permatasari, Dian Ayu Poedji Hastutiek Poppy Rahmatika Primandiri Pratama, Bima Putra Rahadyan, Raihan Alif Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmi Sugihartuti, Rahmi Ramadhani, Fifi Fauziah Rimayanti Rimayanti Riwu, Katty Hendriana Priscilia Rizhal Hendi Ristanto Rochmah Kurnijasanti Rodprasert, Watchareewan Rosyta, Pegy Sabdoningrum, Emy Koestanti Saraswati, Afif Tasya Setiawan Koesdarto Setyowidodo, Irwan Shafina Hanum, Farah Siti Aizah Sri Chusniati Suwarno Suwarno Suwarno Tacharina, Martia Rani Tita Damayanti Lestari Tri Julianto Tyasningsih, Wiwiek Widjiati Widjiati, Widjiati Wijaya, Andi Yasmin Wiwiek Tyasningsih Wiwik Misaco Yuniarti Yeni Dhamayanti