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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Folia Medica Indonesiana Indonesian Journal of Cancer Universa Medicina Journal Of Vocational Health Studies Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Medik Veteriner journal of Basic Medical Veterinary Current Biomedicine
Widjiati Widjiati, Widjiati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Journal : journal of Basic Medical Veterinary

 1 
POTENSI KANDUNGAN ANTIOKSIDAN RUMPUT KEBAR (Biophytum Petersianum Klotzsch) TERHADAP APOPTOSIS DAN GAMBARAN HISTOPATOLOGIS TESTIS PADA MENCIT (MUS MUSCULUS) YANG DIPAPAR 2,3,7,8-Tetrachlorodibenzo-p-dioxin Dewita, Dewita; Widjiati, Widjiati; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Hariadi, Mas'ud; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19986

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic compound in the dioxin group. This compound is a pollutant for the environment and very harmful to human health and enter the body through the mucous membranes in the mouth and the respiratory tract and can be transmitted through the placenta and lactation. The aim of this study was to know the antioxidantpotency of to resolve reproduction disturbance caused by TCDD exposure.Thirty Balb/C male mice were divided into five different groups, the negative control group, a positive control group exposed to TCDD at a dose of 7 μg/kg BW, P1 group of groups exposed to TCDD doses of 7 μg / kg BW and given Biophytum petersianum extract 0.05mg/gBB/day, group P2 group exposed to TCDD doses of 7μg/kgBW and given 0.080mg/gBB/day, and group P3 were exposed to TCDD dose of 7μg/kgBB and given the extract of Biophytum petersianum 0.135mg/gBB/day during day 2 to day 55. On the 56th day the mice were sacrificed and apoptotic examination and spermatogenic cell histopathological features were performed on the testis. The results showed that: P2 (0,433 ± 0,497; p<0,05) and P3 (0,200 ± 0,000 p<0,05) groups were the most effective group in decreasing spermatogenic cell apoptosis compared to positive control group (2,933 ± 1,5832 p<0,05). The Johnsen score result showed that P2 (9,400 ± 0,420) and P3 (9,800 ± 0,253) groups improving the histopathologic picture of spermatogenic cells in seminiferous tubules compared to positive control group (7,20 ± 0,400) p<0,05. Conclusion of this study were Biophytum petersianum is effective to solve reproduction disturbances caused by exposure of TCDD and the P3 group is the most effective group.
DESCRIPTION OF HEMATOLOGICAL VALUE IN LEOPARD GECKO (Eublepharis macularius) Wiranata, Try Ardhi; Widjiati, Widjiati; Wahyuni, Retno Sri; Hidajati, Nove; Poetranto, Emmanuel Djoko; Yuliani, M. Gandul Atik
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19990

Abstract

The aim of this research was to explore the hematological value of leopard gecko (Eublepharis macularius). Total of 20 samples were examined, its consists of 10 males and 10 females, blood samples were taken from vena ventral abdominal and deposited into EDTA tubes, fresh blood leaves little to make blood smear, total blood examination conducted with automatic hematological analyzer ABX MICROS 60 and for total leukocyte and differential leukocyte count used standard manual method. The mean result of hematological value of leopard gecko in this research are WBC (x103/mm3) 7,12 male, 8,09 female, Lymphocyte (%) 46,2 male, 38,4 female, Monocyte (%) 17 male, 17,3 female, Heterophil (%) 18,3 male, 21,8 female, Eosinophil (%) 10,8 male, 13,9 female, Basofil (%) 7,7 male, 8,6 female, RBC (106/mm3) 0,64 male, 0,65 female, HGB (g/dl) 8,95 male, 9,46 female, Hematocrit (%) 10,55 male and 9,26 female. In all parameters showed that hematology value of male gecko leopard was lower than female gecko except on two parameters which are hematocrit and lymphocyte which showed male gecko has the higher value than females, but the hematology value difference of both sexes leopard gecko is not significant except in heterophil value. 
PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL SERTOLI MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Berlina, Cyrcilia Relita; Eliyani, Hana; Sami, Abdul; Widjiati, Widjiati; Mulyati, Sri; Anwar, Chairul
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19991

Abstract

This study  was aimed  to know  the effect of Kebar grass  extract  on amount of mice  Sertoli  cell exposed  by TCDD.  This study was  experimental   study  with completely   randomized  design.  Thirty adult  male  mice  of Mus  musculus strain Balb/C,  age 11 weeks  and  weight   25 - 30 ~were    used.  Mice were divided   into five groups,   that  were:  K- was  control  (aquadest   0)   mL); K+ was  injected  with TCDD  at  dose  of 7 µg/kg   BW;  Pl  was  injected  with TCCD  and  administrated with   Kebar   extract   (0,045 mg/  g  BW/day);   P2 was   injected   with   TCCD  and administrated  with  Kebar  extract  (0,080 mg/ g BW/day);   P3 was  injected  with TCDD and  administrated with  Kebar extract  (0,135 mg/  g BW/day).   Kebar grass extract  was  administrated in 53 days.  Mice were sacrificed  and  right  testis organs were  taken.  Then, histology   preparat  with  HE  staining   were  made  and  Sertoli cells  were  counted.    Data were   analyzed  by  One  Way  ANOV A  followed   by Duncan test  (a  =  0,05).   The result  of this  study   showed that  administration  of Kebar  grass  extract  affected  amount  of mice sertoli  cells.  K+ (2,92a±0,109) showed significance difference  (p<0,05) compared   to Pl (5,00b±0,374), P2 (7,64c±0,409)  and P3 (9,68ct±0,363).  Meanwhile, amount   of Sertoli cells of P3 at the highest  dose  did not  show  significance difference   (p>0,05)  with  K-  (10,16<l±0,829).  The conclusion of this study was  administration of Kebar  grass  extract  per  oral in 53 days  could maintain amount  of mice Sertoli cell exposed  by TCDD. 
EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Nursadida, Milla; Plumeriastuti, Hani; Dhamayanti, Yeni; Widjiati, Widjiati; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

Abstract

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Jeanita, Winny; Meles, Dewa Ketut; WIdjiati, Widjiati; Hamid, Iwan Sahrial; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

Abstract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.
Co-Authors Achmad Salman Faridz, Achmad Salman Agung Budianto Achmad, Agung Budianto Amila, Ukhti Mukminah ilmi Annise Proboningrat Ardy, Carolina Arimbi Askandar Tjokroprawiro Bambang Poernomo Berlina, Cyrcilia Relita Budi Wicaksono Chairul Anwar Dewita Dewita Eighty Mardiyan K, Eighty Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Erry Gumilar Dachlan, Erry Gumilar Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Hermanto Hermanto Hermanto Tri Joewono Hidajati, Nove Hidayatullah, Amiruddin IMAM MUSTOFA INDRA YULIATI, INDRA Iwan Sahrial Hamid Jeanita, Winny Jojor Sihotang Kuncorojakti, Suryo Lilik Maslachah Luqman, Epy Muhammad Mafruchati, Maslichah Meles, Dewa Ketut Muninggar, Laili Ngakan Made Rai Widjaja Nurbayatin, Anita Nursadida, Milla Nusdianto Triakoso Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Sugianto, Paulus Permatasari, Dian Ayu Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Relly Yanuari Primariawan, Relly Yanuari Rimayanti Rimayanti Rochmah Kurnijasanti Rusyawardani, Aldis Ingrid Sami, Abdul Shabira, Zahra Sri Mulyati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistiawati Sulistiawati Suzanita Utama Tita Damayanti Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Viski Fitri Hendrawan Wahyuni, Retno Sri Widya Paramita Lokapirnasari Wiranata, Try Ardhi Yeni Dhamayanti Yudhana, Aditya Yudiwati, Rina Yulianna Puspitasari