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All Journal Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Ilmu Ternak Zuriat REKA KARSA Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri AGRIVITA, Journal of Agricultural Science JAWI : Journal of Southeast Asia Islamic Contemporary Issues Seminar Nasional Informatika (SEMNASIF) Jurnal Akta Jurnal Penelitian Pertanian Tanaman Pangan Journal of Tropical Crop Science Journal of Maternity Care and Reproductive Health Mudir : Jurnal Manajemen Pendidikan Global Political Studies Journal Bada'a: Jurnal Ilmiah Pendidikan Dasar Jurnal Penelitian Tanaman Industri (Littri) JOURNAL SCIENTIFIC OF MANDALIKA (JSM) JURNAL PENDIDIKAN IPS Perspektif Edusentris: Jurnal Ilmu Pendidikan dan Pengajaran Prosiding Seminar Nasional Program Pengabdian Masyarakat Jurnal Ilmu Sosial dan Humaniora Info Kripto Journal of Comprehensive Science Journal of Law, Poliitic and Humanities Edu Research : Jurnal Penelitian Pendidikan Abdi Jurnal Publikasi Pandawa : Pusat Publikasi Hasil Pengabdian Masyarakat Jurnal Ilmiah Multidisiplin Indonesia Jurnal Kesehatan Siliwangi Jurnal Keperawatan Indonesia Florence Nightingale RESLAJ: Religion Education Social Laa Roiba Journal Jurnal Ar Ro'is Mandalika (Armada) Indonesian Health Literacy Journal (IHLJ) International Journal of Educational Research Konsensus: Jurnal Ilmu Pertahanan, Hukum dan Ilmu Komunikasi Asian Journal of Multidisciplinary Research Business, Entrepreneurship, and Management Journal balarea
Setiawan, Asep S
Department Of Agronomy And Horticulture, Faculty Of Agriculture, IPB University Jl. Meranti, IPB Dramaga Campus, Bogor 16680, Indonesia
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Journal : Jurnal AgroBiogen

 1 
Keterpautan 23 Marka Mikrosatelit pada Kromosom 6 dan 7 dengan Karakter Ketahanan Populasi Jagung terhadap Penyakit Bulai (Peronosclerospora maydis) Roberdi, Roberdi S; Aswidinnoor, Hajrial S; Setiawan, Asep S; Sutrisno, Sutrisno S; Pabendon, Marcia B; Azrai, Muhammad S
Jurnal AgroBiogen Vol 6, No 1 (2010): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n1.2010.p10-17

Abstract

Linkage of 23 Microsatellite Marker on Chromosome 6and 7 to Downy Mildew Resistance on Maize. Roberdi,Hajrial Aswidinnoor, Asep Setiawan, Sutrisno, Marcia B.Pabendon, and M. Azrai. Downy mildew caused byPeronosclerospora is one of most important maize diseasein several countries, including Indonesia. Parental andprogenies selection based on conventional breeding is timeconsuming and laborious. Development of molecularbiology produces many DNA markers used for selection, oneof them is microsatellite. The aim of this research to identifymicrosatellite markers associated with downy mildewresistance on maize progeny MR-4 X AMATLCOHS-9-1-1-1-1-1-2-B, on chromosome 6 and 7. This research was consistedof two activities, phenotypic and genotypic analysis.Phenotypic analysis used 175 progenies BC1F2 and both ofparents. This analysis included planting of spreading row,inoculums preparation, inoculation of spreader rows, testmaterial planting, inoculation of test material andobservation. Genotypic analysis used 175 progenies BC1F1and both of parents. This analysis included DNA genomeisolation, PCR analysis, electrophoresis, gel staining and datascoring. Percentage of downy mildew infections on MR-4was 76%, while these on AMATLCOHS-9-1-1-1-1-1-2-B was16%, and on 175 progenies had range from 10.1-100%. Out of23 SSR, 12 markers could be mapped in chromosome 6 and11 markers in chromosome 7. QTL analyses showed thatchromosome 7 contain one QTL in position between phi082and phi116I marker as far as 18.6 cM with 2.6 LOD value.
Gen Pengendali Sifat Ketahanan Penyakit Blas (Pyricularia grisea Sacc.) pada Spesies Padi Liar Oryza rufipogon Griff. dan Padi Budi Daya IR64 Dwinita Wikan Utami; Sugiono Moeljopawiro; Hajrial Aswidinnoor; Asep Setiawan; Ida Hanarida
Jurnal AgroBiogen Vol 1, No 1 (2005): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n1.2005.p1-6

Abstract

Improvement of rice for durable resistance rice blast is difficult due to the complexity of the inheritance of resistance. As study was conducted to analyze blast resistance in rice using two different approaches, i.e., blast QTL mapping and comparison of resistance spectrum and genetic control. The blast QTL mapping was done using an interspesific population originated from backcrossing between a wild rice, Oryza rufipogon, and a cultivated rice, IR64. Comparison of resistance spectrum and genetic control was based on phenotypic reactions. Results of the experiment showed that based on the blast QTL mapping, genes Pirf2-1(t) and Pir2-3(t) were mapped on chromosome 2. Gene Pirf2-1(t) was isolated from chromosome 2 of O. rufipogon and coding for resistance to P. grisea race 001, while gene Pir2-3(t), which was isolated from rice cultivar IR64, was coding for resistance to P. grisea race 173. Based on the resistance spectrum, O. rufipogon has a non-race specific resistance. Gene Pirf2-1(t) on O. rufipogon contributed a dominant mode of resistance to blast which was affected by a duplicate epistasis. The other gene, Pir2-3(t) contributed an additive mode of resistance which was affected by a complementary epistasis.
Co-Authors . Giyanto Adi Tia Setiawan Ady Kusuma Nurcholis Afifah Farida Jufri Aghus Jamaludin Kharis Agus Purwantara Agustian, Yanyan AJAR DIRGANTORO Ajeng Amanda Thayeb AK Sembiring, Desy Aldebaran Raihan Revidy ALEX HARTANA Alfianta Cesario Rizky Maulana Alma Ghina Halimah Anah Sasmita Andi Ismira Andreas Tan Aris Purwanto Arya Panca Asroni Asroni Ayu Puspita Ningrum Azrai, Muhammad S Bambang Eko Widyanto BAMBANG HELIYANTO Bambang Heliyanto Bambang Heliyanto BAMBANG HELIYANTO Bani Sakti Bayu Brahmantia Betty Mauli Rosa Bustam Cecep Effendi Darmawan Saptadi Darmawan Saptadi Darmawan Saptadi Devi Novianti Dhanang Ajie Dinar Meidiana Dindin Abidin DONATA S PANDIN Dwi Putri Maulida Dwinita W. Utami Dwinita Wikan Utami Edi Guhardja Elfan Fanhas Fatwa Khomaeny Endang Sulastri Erwin Yuniar Rahadian Fadli Wahab Frederico Tunggal Gina Rahma Utami Gunanto , Djoni Gunarto Gun H. Hardhienata HAJRIAL ASWIDINNOOR Hamka Hamka Hanan Dwiky Masshafy Hani Handayani Haris Aprianto Setiawan Harmonis Hendrik Syaputra Heri Budiawan Ida Hanarida Indarini Dwi Pursitasari Iriana Bakti Iswara, Vidya Itkonul Akbar Kadeni Kadeni, Kadeni Kamajaya, Ryan Muhammad Azizulfiqar Laode Muhamad Fathun Lilis Lismayanti Maesaroh Lubis Megayani Sri Rahayu Memen Surahman Memen Surahman Miftahul Falah Miftahul Ulum Muhamad Agung Dharmajaya Muhamad Asykal Rizki Muhamad Fikri Dzam-Dzam Muhammad Isa Asyrofuddin Mujiarto Mujiarto Nadya Paramitha Nandang Ahmad W Nandang Ahmad Waluya Naufal Fauzi Akbar Neni Nuraeni Neni Sholihat Nofirman Nurhajati A. Mattjik Nurhajati A. Mattjik Nurhalim Nuri Aslami Pabendon, Marcia B Palupi, Endah R. Pito Fernando Puspa Khoerun Nisa R r. SRI HARTATI R.R. SRI HARTATI Reni Indrayanti Rino Vanchapo , Antonius Risma Rahmawati Rizka Putra Roberdi, Roberdi S Rr Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Saepudin Sarijan, Abdullah - Septina Putri Laia Setiani, Revina Putri Slamet Riyadi Sony Sumaryo Sri Mulyanti Sri Yunanto Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO SUDARSONO SUGIONO MOELJOPAWIRO Sugiono Moeljopawiro Suhartanto, Muhammad Rahmad Sulastri Rini Rindrayani Surti Kurniasih Surtikanti Sutrisno, Sutrisno S Suwarto Suwarto Syahnanto Noerdin Tisngatul Aliyah UBHI, Trias Inang Novanti Usni Wahyu Hidayat Waluya, Nandang Ahmad Yanyan Mochamad Yani Yudha Purwanto