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Rahmawati, Siti Irma
Universitas Djuanda
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Aktivitas Antioksidan Kapang Endofit asal Rumput Laut Caulerpa lentilifera Septiana, Eris; Hardhiyuna, Mutia; Untari, Febriana; Bustanussalam, Bustanussalam; Rahmawati, Siti Irma
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 20, No 1 (2025): Juni 2025
Publisher : Politeknik - Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v20i1.1040

Abstract

Aktivitas antioksidan dari ekstrak rumput laut telah banyak dilaporkan. Namun, belum banyak peneliti yang melaporkan aktivitas antioksidan dari kapang endofit asal rumput laut. Penelitian ini bertujuan untuk mengisolasi kapang endofit dari rumput laut C. lentilifera, mengevaluasi aktivitas antioksidan, serta profil senyawa kimia ekstrak terpilih. Isolasi kapang endofit dilakukan dengan metode sterilisasi permukaan dan ditanam pada media Potato Dextrose Agar (PDA). Uji antioksidan menggunakan metode 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), dan ferric reducing antioxidant power (FRAP). Penentuan kadar fenolik dan flavonoid total masing-masing berdasarkan pada reaksi reagen Follin-Ciocalteu dan aluminium klorida. Ekstrak dengan aktivitas antioksidan terbaik dilakukan profiling senyawa kimianya menggunakan Kromatografi Gas-Spektroskopi Massa (KG-SM). Hasil isolasi didapatkan lima isolat kapang endofit yaitu Cl.Tka.S1, Cl.Tka.S2, Cl.Tka.S3, Cl.Tka.R1, dan Cl.Tka.R2. Ekstrak kapang endofit Cl.Tka.S3 memiliki aktivitas tertinggi dengan nilai IC50 sebesar 17,65 ± 1,88 dan 37,07 ± 2,16 mg/L pada metode ABTS dan DPPH,serta uji FRAP dengan kadar setara Fe(II) sebesar 1654,15 ± 32,95 µmol/mg ekstrak. Kadar fenolik total tertinggi didapatkan dari ekstrak kapang endofit Cl.Tka.S1 sebesar 75,05 ± 8,46 mg setara asam galat/g ekstrak. Kadar flavonoid total tertinggi didapatkan dari ekstrak kapang endofit Cl.Tka.S3 sebesar 38,50 ± 1,55 mg setara kuersetin/g ekstrak. Profiling senyawa kimia menunjukkan ekstrak isolat Cl.Tka.3 sebagai isolat terbaik mengandung senyawa antioksidan berupa senyawa golongan fenolik, asam lemak, alkana, dan alkohol. KATA KUNCI: ABSTRACTThe antioxidant activity of seaweed extracts has been widely reported. However, reports on the antioxidant activity of endophytic fungi derived from seaweed are still limited. This study aims to isolate endophytic fungi from C. lentilifera, determine antioxidant activity and chemical profiles of selected extracts. Endophytic fungi were isolated by the surface sterilization method and planted on Potato Dextrose Agar (PDA) media. Antioxidant test using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), dan ferric reducing antioxidant power (FRAP) methods. Total phenolic and flavonoid content was based on the reaction of Follin-Ciocalteu reagent and aluminium chloride, respectively. Extracts with the best antioxidant activity were profiled for their chemical compounds using Gas Chromatography-Mass Spectroscopy (GC-MS). The isolation results obtained five isolates of endophytic fungi, namely Cl.Tka.S1, Cl.Tka.S2, Cl.Tka.S3, Cl.Tka.R1, and Cl.Tka.R2. Cl.Tka.S3 extract had the highest activities with an IC50 value of 17.65 ± 1.88 and 37.07 ± 2.16 mg/L on the ABTS and DPPH methods, as well as FRAP test values with Fe(II) equivalent levels of 1654.15 ± 32.95 µmol/mg extract. Cl.Tka.S1 extract has the highest total phenolic content of 75.05 ± 8.46 mg gallic acid equivalent/g extract. Cl.Tka.S3 extract has the highest total flavonoid content of 38.50 ± 1.55 mg quercetin equivalent/g extract. Profiling of chemical compounds showed that the Cl.Tka.S3, as the best extract, contained antioxidant compounds such as phenolic compounds, fatty acids, alkane, and alcohol.
Anti-inflammatory Activity of Glucomannan Resulting from β-mannanase Enzyme Hydrolysis in RAW 264.7 Cells Nuraini, Nuraini; Effionora Anwar; Dian Ratih Laksmitawati; Hendig Winarno; Siti Irma Rahmawati
Journal of Natural Product for Degenerative Diseases Vol. 2 No. 2 (2025): JNPDD March
Publisher : Faculty of Pharmacy Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58511/jnpdd.v2i2.7797

Abstract

Glucomannan is a polysaccharide of the hemicellulose type, composed of glucose and mannose chains. It has the ability to form solutions that expand and thicken. The bioactivity of carbohydrates is influenced by the length of their saccharide chains. Hydrolysis can alter the structural dimensions of polysaccharides, including molecular weight, type, number, and position of substituent groups, which in turn impacts their biological activity. Various methods can modify carbohydrate structures to enhance their value, with degradation being one such method. Enzymatic degradation is particularly advantageous due to its high specificity, efficiency, and minimal side effects. Numerous studies have identified glucomannan as having immunomodulatory effects on macrophage cells. When stimulated with lipopolysaccharides, macrophages release various cytokines and inflammatory mediators, such as TNF-α, iNOS, and NO. This study explored the potential anti-inflammatory effects of glucomannan (GM) and its enzymatically hydrolyzed form (Enz-GM) on RAW 264.7 cells. The research involved degrading glucomannan using the β-mannanase enzyme, followed by in vitro testing of the degradation product (Enz-GM) for anti-inflammatory activity. This was achieved using an ELISA kit to measure the expression of IL-6, TNF-α, and iNOS. The results showed that Enz-GM at pH 9 (Mw 1.24 kDa) significantly reduced the pro-inflammatory cytokines IL-6 and iNOS (p ≤ 0.05) compared to GM, while TNF-α expression did not show a significant difference between Enz-GM and GM.
OPTIMASI SELULASE PADA ENZYME ASSISTED EXTRACTION LEMAK DARI Caulerpa lentillifera SEGAR MENGGUNAKAN RESPONSE SURFACE METHODOLOGY Hanifah, Ishmah; Izzati, Fauzia; Rahmawati, Siti Irma; Hermanianto, Joko; Giriwono , Puspo Edi; Hapsari , Yatri; Bustanussalam; Rahman , Fauzy; Septiana , Eris; Simanjuntak , Partomuan
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 13 No. 1 (2021): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v13i1.32654

Abstract

Caulerpa lentillifera merupakan rumput laut hijau yang memiliki potensi besar namun kelimpahannya belum banyak dieksplorasi. Rumput laut tersebut tersebar pada beberapa wilayah perairan di Indonesia. Rumput laut diketahui memiliki kadar lemak yang rendah namun tersusun oleh poli asam lemak tidak jenuh. Ekstraksi lemak pada umumnya hanya menggunakan pelarut organik. Pada proses ekstraksi diperlukan perlakuan awal seperti enzyme assisted extraction untuk mendegradasi dinding sel dan meningkatkan akses pelarut masuk ke dalam sel. Penelitian dirancang untuk mengetahui kondisi optimum proses enzyme assisted extraction lemak rumput laut hijau segar C. lentillifera dengan menggunakan enzim selulase. Proses optimasi dilakukan menggunakan Response Surface Methodology (RSM) model Central Composite Design dengan 15 perlakuan. Perlakuan didapatkan untuk mengetahui pengaruh variabel bebas, diantaranya konsentrasi enzim, suhu hidrolisis, dan waktu hidrolisis terhadap variabel terikat yaitu jumlah ekstrak lemak dan aktivitas antioksidan. Hasil penelitian didapatkan model 2FI dan Linier berturut-turut untuk variabel terikat jumlah lemak dan aktivitas antioksidan. Kondisi optimum yang diperoleh yaitu konsentrasi enzim sebesar 2%, suhu hidrolisis sebesar 30 °C, dan waktu hidrolisis selama 1 jam. Kondisi optimum tersebut kemudian dapat diverifikasi dengan diberikan perlakuan terpilih sebanyak 2 kali ulangan atau lebih hingga mendekati hasil prediksi. Asam lemak yang diperoleh setelah metilasi dan identifikasi dengan GC-MS yaitu asam palmitat dan asam laurat. Optimasi proses ekstraksi lemak memungkinkan potensi pemanfaatan lemak dari rumput laut segar C. lentillifera berdasarkan faktor yang memengaruhi.
Co-Authors . Anidah . Aslan . Kholik A.A. Ketut Agung Cahyawan W Abdullah, Muhamad Al Faruq Afifah Rosyidah Afifah, Diana N. Ahmad Syauqy Andreayani Attamimi Anggun Hari Kusumawati Anis H. Mahsunah Anisyah, Yuni Nur Anjani, Gemala Annis Catur Adi Annisa Rizky Maulidiana Apon Zaenal Mustopa Apon Zaenal Mustopa Ardiana, Hana Ai Ariestyani, Imelda Armaiki Yusmur Arrachman, Hajar Chair Arrahman, Hajar Chair Aulanni'am, Aulanni'am Aulanni’am Aulanni’am Bayu, Asep Bugi Ratno Budiarto Bustanussalam Bustanussalam Bustanussalam Bustanussalam Bustanussalam Bustanussalam Bustanussalam Bustanussalam Bustanussalam, Bustanussalam Chianese, Giuseppina Choirun Nisa Riski Damayanti, Aprilia F. Darmawati Dalle Dharmawan, Devi Diah Anggraini Wulandari Dian Ratih Laksmitawati Diana Nur Afifah, Diana Nur Donald, Eriklex Dwi Aryanti Effionora Anwar Elke Stephanie Ella Saparianti Elok Zubaidah Endang Mahati Endang Saepudin Eris Septiana ERIS SEPTIANA Eris Septiana Eris Septiana Eris Septiana Eris Septiana Eris Septiana Fadhilah, Syifa Fadlina Chany Saputri Fariha Willisiani Fauzia Nurul Izzati Fauzia Nurul Izzati Fauzia Nurul Izzati Fauzia Nurul Izzati Fauzia Nurul izzati Fauzia Nurul Izzati Fauzia Nurul Izzati Fauzia Nurul Izzati Fauzy Rachman Fauzy Rachman Fauzy Rachman Fauzy Rachman Fauzy Rachman Febriandy, Agustio Febriansyah, Ilham Ferdina Tri Laksmita Fikayuniar, Lia Fitri Amalia Fitri Nur Hikmah Fitrilia, Tiana Ghina Putri Dyanti Giriwono , Puspo Edi Gita Syahputra Hadistio, Alfin Hairida Hairida Hanifah, Ishmah Hapsari , Yatri Hendig Winarno Heni Pujiastuti Indradewa, Rhian Izzati, Fauzia Joko Hermanianto Karimah, Nur Rafidah Kharyani, R. Aldini Kristanti Indah Purwani Leny Heliawati Leo, Leviena Merlynike Lestari Makmuriana Lutfi Alhazami MADE ASTAWAN Mardiah Marhamah, Irma Siti Martha Sari Martha Sari Masteria Yunovilsa Putra Masteria Yunovilsa Putra Muhammad Ilman Muhammad Surya Madani Muhibuddin, Anton Mutia Hardhiyuna, Mutia Nada Nisrina Nastiti Wijayanti Nelwan, Sindy Cornelia NFN Bustanussalam NFN Yadi Noli Novidahlia Nopita Aliani Nunik Gustini Nur Rohmah Nuraini Nuraini Nurcahali, Fani Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Permatahati, Ghina Sri Putra, Masteria Yunovilsa Qisthina, Dalilah Rachmat Sahputra Rahma Micho Widyanto Rahman , Fauzy Ramadhaniyati, Ramadhaniyati Ratna Chrismiari Purwestri Ratna Ediati Rika Damayanti Rikno Harmoko Rikno Harmoko Rikno Harmoko Rosy Hutami Saiful Bachri Salcia Inka Nelanda Saniyya, Ashila Nasyadhiya Septiana , Eris Setyawati, Amalia Rani Simanjuntak , Partomuan Siregar, Maruli Siti Aminah Siti Nurhalimah Siti Nurjanah Slamet Budjianto SOETJIPTO . Soetjipto Soetjipto Sukarno Sukarno Sunaryo, Endang S. Suprayatmi, Mira Suprayatmi, Mira Syamsiah Syamsiah Tirtawati, Desi Titi Rohmayanti Tutik Wresdiyati Udijanto Tedjosasongko Untari, Febriana Utami, Nur' Widjajanti, Widjajanti Wien Kusharyoto Wyna Nabila Yadi Yadi Yatri Hapsari Yatri Hapsari Yatri Hapsari Yatri Hapsari Yohana P. Br Sianturi Yuliana, Resti Yuliana, Resti Yumi Sartika Yunas, Ranum Wanudya