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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Teknik Industri Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Majalah Ilmiah Peternakan Jurnal Kedokteran Brawijaya JURNAL SAINS DAN TEKNOLOGI INDONESIA Biotropika Jurnal Ilmu Kimia Universitas Brawijaya Jurnal Teknologi Pertanian Proceeding International Conference on Global Resource Conservation Natural B The Journal of Pure and Applied Chemistry Research Indonesian Journal of Human Nutrition SEMIRATA 2015 agriTECH Indonesian Journal of Biotechnology Suhuf Animal Production : Indonesian Journal of Animal Production Journal of Applied Food Technology Islamika : Jurnal Agama, Pendidikan dan Sosial Budaya Jurnal Ilmiah Kesehatan (JIKA) Jurnal Kedokteran Hewan

Aulanni'am, Aulanni'am

Universitas Brawijaya

Author-ID : 1231258

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Therapy of Lactobacillus casei Shirota strain Fermented Milk to Rats with High-Cholesterol Diet toward Cholesterol Levels and SOD Activity Alifiani Nur Rohma; Chanif Mahdi; Aulanni’am Aulanni’am
The Journal of Pure and Applied Chemistry Research Vol 6, No 2 (2017): Edition of May-August 2017
Publisher : Chemistry Department, The University of Brawijaya

This study aimed to determine the fermented milk of Lactobacillus casei Shirota strain potential on cholesterol levels, SOD activity, LPL enzyme activity, and cardiac histopathology. This study used 25 rats (Rattus novergicus) Wistar strain aged 2-3 months, weighing 150-200 grams. These rats were divided into 5 groups: negative control group, positive control group with high-cholesterol diet for 21 days, and therapy group with 1.0 ; 1.5 ; 2.0 mL/rat/day of fermented milk for 14 days. The analysis methods used in this study are One Way ANOVA and Tukey. The results showed that the fermented milk of Lactobacillus casei Shirota strain had a strong effect on blood cholesterol levels and increasing the SOD activity. This study also showed that the fermented milk of Lactobacillus casei with 2.0 mL/rat/day is the most effective dose to decrease the total cholesterol and increase the SOD activity.

Study of Antocyanins Activity from Purple Sweet Potato for Reducing Apoptotic Cells Expression of The Cerebellum On Ischemic Stroke Rats Latifah Tribuana Dewi; Made Oka Adnyana; Chanif Mahdi; Sasangka Prasetyawan; Arie Srihardyastutie; Aulanni’am Aulanni’am
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

This study aims to determine anthocyanin antioxidant performance with a dose of 2 cc/day based on the expression of apoptotic cells on ischemic stroke rats cerebellum. Research was conducted using ischemic stroke rats (Rattus norvegicus) that prepared by ligated for 3 h on the Common Carotid Artery (CCA) and External Carotid Artery (ECA) followed by reperfusion that commonly known as MCAO (Middle Cerebral Artery Occlusion). The anthocyanin extract was characterized by LC-MS and its IC50 was measured by DPPH method. The rats were divided into five groups 1) negative control; 2) reperfusion 1 h; 3) reperfusion 72 h; 4) reperfusion 24 h, with anthocyanin therapy; 5) reperfusion 72 h, with anthocyanin therapy. The results of LC-MS showed that anthocyanin from purple sweet potato extracts contained Petunidin-3,5-O-diglucoside (Pt-DG) with an IC50 value of 22.16 μg/mL, categorized as very strong antioxidant. The results showed that apoptotic cells expression of cerebellum decreased significantly (p<0.01) after 72 h reperfusion with anthocyanins therapy until 2.42%. The current work proved that anthocyanin extract effectively suppresses the apoptotic cell’s expression of the cerebellum on stroke ischemic rats.

Characterization of Polyclonal Antibody Induced by Autoantibody TPO (Thyroidperoxidase) From Autoimmune Thyroid Disease (AITD) Serum with ELISA and Western Blotting Maulidya Aulia Fiqriyana; Aulanni'am Aulanni'am; Anna Roosdiana
The Journal of Pure and Applied Chemistry Research Vol 2, No 1 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Autoantibody TPO is a potential marker for early detection of autoimmune thyroid disease (AITD). Autoantibody TPO has a specifity and a sensitivity ranging from 82% to100% in comparison to other AITD serology markers. Concentration of autoantibody TPO in sera had a positive correlation with activities of chronic AITD. This research have been conducted to investigate the characteristic of polyclonal antibody TPO induced by autoantibody TPO from serum of AITD patients. The autoantibody TPO was isolated from serum of AITD patients and its band proteins was confirmed using western blotting, acquiring molecular weight of 51.81 kDa. The protein was induced to a rabbit (Oryctolagus cuniculus) and recognized as an antigen. Serum of rabbit produced polyclonal antibody which was characterized using ELISA and Western blotting. The result showed that autoantibody TPO isolated from serum of AITD patients was immunogenic, which induced the polyclonal antibody anti-TPO (anti-anti-TPO). It was found that the highest polyclonal antibody was obtained on 3rd serum bleeding with the absorbance of 0.3225 and a molecular weight of 51.81 kDa, which is able to specifically recognize autoantibody TPO. Keywords : Autoimmune thyroiditis, Autoantibody TPO, TPO, polyclonal antibody, ELISA, Western blotting

A Study on Brown Seaweed Therapy ( Sargassum sp.) toward MDA Levels and Histological Improvement on Rat Foot Suffering Rheumatoid Arthritis Fauziah Fauziah; Aulanni'am Aulanni'am; Chanif Mahdi
The Journal of Pure and Applied Chemistry Research Vol 2, No 3 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Rheumatoid arthritis (AR), an autoimun disease, is characterized by the inflammation in the joint area caused an excessive of free radicals. An excessive of free radicals in the body cause oxidative stress, that increasing levels of malondialdehyde (MDA) as an indicator of lipid peroxidation and the decreasing levels of anti-oxidants. The treated with extract of brown seaweed (Sargassum sp.) intended to find out the MDA levels in serum and the histological of the foot joints rheumatoid arthritis rats. Malondialdehyde levels are determined through a TBA test (Thio Barbituric acid), meanwhile the histological of the rat foot joints was determined by Hematoxylen-Eosin staining (HE). The results showed the brown seaweed extract therapy (Sargassum sp.) was significantly (p <0.01) reduce levels of malondialdehyde (MDA) in the serum of 21,24% and improving histological foot joints rheumatoid arthritis rats.

Effects of Ruellia tuberosa L. Root Extract on the Pancreatics of Diabetic Rat Muhammad Asadullah; Arie Srihardyastutie; Aulanni'am Aulanni'am
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Diabetes mellitus type 1 (DMT1) is an insulin deficiency disorder caused by pancreatic damage characterized by chronic hyperglycemia. Animal modeling of DMT1 using inducting Multiple Low Dose Streptozotocin (MLD-STZ) elevat levels of free radicals and inflammation in pancreatic organs which then damage β cells. Ruellia tuberosa L. is a plant containing triterpenoids from the class of antioxidant and anti-inflammatory flavonoids. This study used three groups of rat: negative control group, positive control and 240 mg/kg bw therapy group. Study are based on changes MDA levels, blood glucose and histologic of pancreas. MDA level determining with TBA method, glucose levels using EasyTouch GCU and histopalogy of pancreas using Hematoxylin-Eosin staining. Statistical analysis of MDA and blood glucose (α = 0.05) showed significant decreases in MDA and blood glucose levels. Based on the results of histologic observations of pancreas descriptively showed a decreas in cell damage on Langerhans island. Thus, the provision of root extract of Ruellia tuberosa L. can inhibit the damage in rat DMT1 model so that it can be used as therapy of diabetes mellitus.

MDA and Histologic Profile of Pancreatic Diabetic-Rats Model Administered With Extract of Glycine max (L.) Merr. Luh Putu Gina; Aulanni'am Aulanni'am; Chanif Mahdi
The Journal of Pure and Applied Chemistry Research Vol 5, No 1 (2016)
Publisher : Chemistry Department, The University of Brawijaya

Diabetes Mellitus is characterized by leveling up glucose in human blood and affects increasing of free radicals in body as well as leading to cellular oxidative stress. Experimentally, this condition is able to be characterized by increasing malondialdehyde (MDA) level in cell and histological changing in pancreas appearance. Consumption of antioxidant substances was reported able to reduce the MDA quantity as free radicals. Black soybean or Glycine max (L) Merr. was reported contains important antioxidant agents such as anthocyanin and isoflavone. This paper discloses recent investigation on application of black soybean water extract to reduce the MDA level on diabetes mellitus-rat model induced by STZ (DM) and also reports the pancreas histological changing of the DM rats. Investigation revealed that black soybean water extract significantly affect decreasing of MDA level by 4.9%, 27.1% and 45.7% in three different doses theraphy (500, 750, and 1000 mg/kg BW). Histologically, it also clearly indicates repairing of pancreas tissue of the DM rats.

Effect of D-Alpha Tocopherol Therapy towards Malondialdehyde Level and Histology of Kidney in Rattus norvegicus with MLD-STZ Induction Marissa Agnestiansyah Mahardhian; Aulanni’am Aulanni'am; Chanif Mahdi
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Diabetic Nephropathy is a kidney disease which occurs due to complication of diabetes mellitus as a consequence of the damage of the kidney endothelial cells. Hyperglicemia condition in patients with diabetes mellitus that induces an oxidative stress, were related to endothelial cell damage. Oxidative stress as a result of hyperglycemia will activate a number of signal transduction pathways resulting in increase of free radicals. D-alpha tocopherol as one of antioxidant substance, that can act as an inhibitor of free radical chain reactions, play an important role in the reduction of the oxidative stress effect. Effect of D-alpha-tocopherol in reducing oxidative stress is identified by the levels of malondialdehyde (MDA) in kidney and histology of kidney. This study used five groups mice; they were a control group, a diabetic group which was induced with MLD-STZ, and a therapeutic groups with a varieties doses of D-alpha tocopherol (100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW). The results showed that the D-alpha tocopherol was able to reduce the levels of malondialdehyde (MDA) and repair the histology of kidney of mice induced by MLD-STZ.

The Effect Of Juice Mangosteen Rind (Garcinia Mangostana L.) To Blood Sugar Levels And Histological Of Pancreatic Rats With The Induction Of Streptozotocin Maris Kurniawati; Chanif Mahdi; Aulanni'am Aulanni'am
The Journal of Pure and Applied Chemistry Research Vol 3, No 1 (2014)
Publisher : Chemistry Department, The University of Brawijaya

Diabetes Mellitus (DM) is a chronic metabolic disorder characterized by high blood sugar levels as a result of insufficiency of insulin function. Xanton a bioactive compound in the mangosteen rind is convinced to possess antidiabetic effects. There for, in this study effect of juice mangosteen rind (Garcinia mangostana L.) on blood sugar levels, and histological features of pancreatic rats with the induction of streptozotocin was investigated. This research using experimental rats species of Rattus norvegicus Wistar strain male. Rats were divided into 3 groups, the first group was the control without any treatments, group II was sick rat group, and group III was therapy rat group treated with juice mangosteen rind (a dose of 110 mg/kg body weight) by sonde for 2 consecutive weeks. Then,blood sugar levels of all rat groups were measured along with observation of their pancreatic histological. The results showed that the blood sugar level in therapy rat group (104.7 ± 10.9 mg/dL) is not significantly different with that of rat control group (108.5 ± 19.5 mg/dL), revealing effectiveness of therapy. Conservely, blood sugar level remained high in sick rat group (163.8 ± 16.2 mg/dL). Juice mangosteen rind can improve conditions of the pancreatic histology rat with the induction of streptozotocin.

Effect of Topical Aplication of Gel Aloe Vera Extract on The UVB-Induced Skin Photoaging in Hairless Rats Izzatul Lailiyah; Sasangka Prasetyawan; Aulanni'am Aulanni'am
The Journal of Pure and Applied Chemistry Research Vol 6, No 2 (2017): Edition of May-August 2017
Publisher : Chemistry Department, The University of Brawijaya

UVB generates the production of Reactive Oxygen Species (ROS) and decrease antioxidants enzymatic excessively. Both of these biological effects caused photo-aging. Excessive ROS production afforded overexpression of AP-1 as a major regulator of photoaging. This paper figured out the potential of Aloe vera extract as topical gel treatment on the UVB-induced skin photo-aging in twenty male wistar hairless rats (Rattus novergicus) divided into 2 groups. First group was induced by UVB and the second was induced by UVB and topical gel extract Aloe vera treatment. Each group was given treatment for 4 weeks. The expression of MDA and SOD were measured with immunohistochemistry. The result showed that the topical gel therapy decreased the MDA expression and increased SOD expression significantly (p < 0.01). The conclusion from this study was Aloe vera extract had potentially as an alternative topical treatment of photoaging.

Study on iNOS expression and levels of Malondialdehyde in the White Rat (Rattus norvegicus) Exposed with Aggregatibacter actinomycetemcomitans Reviana Setin; Aulanni'am Aulanni'am; Chanif Mahdi
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Aggregatibacter actinomycetemcomitant (Aa) is gram-negative bacteria composed of lipopolysaccharides (LPS) in its outer membrane. This bacteria is normally found in dental plaque and periodontal pockets causing parotid gland damage when it is present in excess amounts. LPS is an endotoxin and a complex glycolipid composed of polysaccharides (hydrophilic) and lipid A (hydrophobic) which can induces Reactive Oxygen Species (ROS), and increase the levels of malonaldialdehyde (MDA) and tissue damage. The aims of this research are to find the expression of iNOS parotid gland cell and to determine levels of MDA using thiobarbituric acid (TBA) spectrophotometric test at 533 nm. The results showed a significantly difference (P<0.05) of MDA levels in control rats (3.135 ± 0.0436 ppm) and Aa rats (6.307 ± 0.0473 ppm). iNOS expression is confirmed by the brown spots formed on the parotid gland, affording the enhancements of iNOS expression in Aa rats and control rats of 2.292 ± 0.038 and 0.166 ± 0.003, respectively (P<0.05). Keywords : Aggregatibacter actinomycetemcomitans, Reactive Oxygen Species, malondialdehyde, iNOS

Co-Authors A. Harijono A.A. Ketut Agung Cahyawan W Achmad Subagio Agung Pramana Warih Mahendra Agung Pramana Warih Marhendra, Agung Pramana Warih Alifiani Nur Rohma Almas Dwi Khairana Alvi Milliana Amiruddin A Anna Roosdiana Anna Roosdiana Annis Catur Adi Annisa Rizky Maulidiana Ardian Retno Sari Arie Srihardyastutie Arman Sayuti Avin Ainur Fitrianingsih Badriyah B Basuki B. Purnomo Basuki B. Purnomo Basuki B. Purnomo Betti Lukiati Binawati Ginting Budi Siswanto Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Charlotha I. Tupan D. Arfiati Darwadi, Rizky Prayoga Deasy Luandayanti Dewi, Devis Resita Diyan Ajeng Rossetyowati Dwinna Aliza E. Y. Herawati Elfi Anis Saati Erdiansyah Rahmi Erdiansyah Rahmi Eva Ari Wahyuni fatchiyah . Fauziah Fauziah Fauziah Usman G Riady, G G.P. Ganda Putra Gatot Ciptadi H. Purnomo Hamdan Hamdan Hendra Susanto I Nengah Kundera I.D. Retnaningtyas Ida Ayu Okarini Inggita Kusumastuty Ira Perdana Wati Izzatul Lailiyah Kurniasari, Balqis L. E. Radiati Lailia Nur Rachma Latifah Tribuana Dewi Luh Putu Gina Luh Putu Gina M. Aris Widodo M. Aris Widodo M. Aris Widodo M.F. Wadjdi Made Oka Adnyana Mahdi, Chanif Marhendra, Agung P.W. Maris Kurniawati Marissa Agnestiansyah Mahardhian Maulidya Aulia Fiqriyana Megawati Sistin Agustita Mudawamah Mudawamah Muhaimin Rifa'i Muhamad Agus Wibowo Muhammad Aris Widodo Muhammad Asadullah Muhammad Hambal Muhammad Hambal Mulyadi Adam Muslim Akmal Muslim Akmal Muslim Akmal Muslim Akmal Nany Suryani Noer Muhammad Dliyaul Haq, Noer Muhammad Dliyaul Nur Ducha Nur Ihsan Nurhaida Nurhaida Nurmahdi, Hilman Nurul Jadid Mubarokati P.S.W, kusuma Prawiro, Sumarno Reto Putri Puspitasari Rachmawati Dwi Agustin Rahma, Kartika Rahmawati, Siti Irma Rara Anggun Mei Nirbaya Retty Ratnawati Reviana Setin Ricadonna Raissa Rista Nikmatu Rohmah Rista Nikmatu Rohmah, Rista Nikmatu Roosdiana, Anna S. Susilowati Sanarto Santoso Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan SIMON BAMBANG WIDJANARKO SOETJIPTO . Soraya Widyasari Soraya Widyasari, Soraya Sri Kumalaningsih Sri Rahayu Sri Wahyuningsih Sri Winarsih Sudiyono Sudiyono Suharti Sukamto Sukamto Sutiman B. Sumitro Sutiman B. Sumitro Syafruddin S T Armansyah, T T Susilawati T. Armansyah THEOVILLA RRD, THEOVILLA Tinny Endang H Tinny Endang Hernowati TN Siregar, TN Tongku N. Siregar Tongku Nizwan Siregar Tri Susanto Vivi Shofia Wibi Riawan Win Darmanto Yenni Risjani Yuli Witono Yulianus Linggi Yustika, Agnes Ratna

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Journal : The Journal of Pure and Applied Chemistry Research