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Journal : Journal of Agritechnology and Food Processing

Review: analysis of optimal conditions of bovine serum albumin (BSA) protein denaturation inhibition method in anti-inflammatory activity testing of various plant leaf extracts Nirmala, Annisa Rizka; Permatasari, Lina; Muliasari, Handa; Deccati, Rizqa Fersiyana
Journal of Agritechnology and Food Processing Vol 3, No 2 (2023)
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jafp.v3i2.20953

Abstract

Inflammation is a manifestation of the immune response to eliminate antigens and a process to respond to tissue damage. There are various drugs to control and suppress inflammation, referred to as anti-inflammatory agents. One method to test anti-inflammatory activity is the inhibition of Bovine Serum Albumin (BSA) protein denaturation. This review article will discuss the stages and optimal conditions in the inhibition of BSA protein denaturation method using various leaf extract samples. Literature was gathered from several journal database websites such as PubMed, ScienceDirect, and Google Scholar with keywords "anti-inflammatory," "inhibition of BSA protein denaturation," and "leaf extract," spanning publication years from 2002 to 2023. The results of anti-inflammatory activity tests can be expressed as either percentage inhibition or IC50 values. Optimal conditions were obtained by preparing a 0.2% BSA solution with Tris Buffer Saline (TBS), maintaining a 9:1 composition ratio between the BSA solution and the sample, and heating at an optimal denaturation temperature of 70 °C. Before conducting tests using this method, it is crucial to ensure that the suspected active compounds with anti-inflammatory activity are not degraded at this heating temperature.
Studi awal uji aktivitas enzim amilase dari tumbuhan secara kualitatif berdasarkan perbedaan suhu dan konsentrasi substrat Muliasari, Handa; Permatasari, Lina
Journal of Agritechnology and Food Processing Vol 2, No 1 (2022)
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jafp.v2i1.9338

Abstract

Amylase enzyme is a starch hydrolyzing enzyme that has wide applications in the food, beverage and biotechnology industries. One source of the amylase enzyme that is easily obtained is plant seeds and sprouts. This research is a preliminary study to qualitatively test the activity of the crude extract of the amylase enzyme from plants. Amylase enzyme was obtained from green bean sprouts, fresh and dried peanut seeds, and fresh and dried corn seeds. The crude extract of the amylase enzyme was tested for its activity using 1% starch as a substrate with variations in temperature and amount of substrate. The result of starch hydrolysis in the form of reducing sugar was detected using Benedict's reagent. The experimental results showed that the amylase enzyme extracted from green bean sprouts and fresh and dried corn kernels showed activity in breaking down starch at 25oC using 6 mL substrate concentration, while dried peanut seeds showed no activity. The enzyme activity is influenced by temperature and the amount of substrate.
Co-Authors Abdul Rohman Aditya Astarika, Yolanda Afriza Faishal, Imam Agrijanti, Agrijanti Aini, Dinatul Alfian G. M, Aji Ali, Baiq Aulia Alya, Ainun Amalia, Marshanda Fitri Anandia Intan Maulidya, Selvira Ananta, Muhammad Naufal Farras Anindiya, Naya Wahyu Annas, Zulfiana Fitrianingrum Ari Khusuma Attaya, Kalisa Aulia Rahmah, Diyani Azariani, Wiwin Budastra, Cintya Bulkaini (Bulkaini) Bulkis, Nanda Cintya Ganes Budastra, Wayan Deccati, Rizqa Fersiyana Dewi, Lale Budi Kusuma Djoko Kisworo Enny Yuliani Farhaini, Azizatul Farobbi, Muhammad Iqbal Fatihatussir, Layla Febrianingsih, Karlina Dwi Fersiyana Deccati, Rizqa Fitriani, Risma Guban Juniarza, A'yuni Hafizah, Gina Tasya Rizka Hafizah, Qori'atul Hajrin, Wahida Haryanto, Wahyu Hayyatun Nufus, Fadirah Hidayati, Agriana Rosmalina Ichsan Ichsan Isnaini, Nisa Istiqamah, Juhratul Junaida, Alfini Juniarza, A'yuni Guban Kabir, Mila Mayanti Lisnasari, Baiq Risky Wahyu M. Rifqi Azami S, Lalu Maesarah, Siti Magfira Nurmalasanti, Nadia Maulidya, Selvira Maulidya, Selvira Anandia Intan Maziya, Raehanul Muchlishah, Neneng Rachmalia Izzatul Mukhlishah, Neneng Rachmalia Izzatul Muliasari, Handa Mustika, Mitha Juliana Ni Made Amelia Ratnata Dewi Ningrum, Linda Tuanida Nirmala, Annisa Rizka Nisa Isneni Hanifa Ocktaviana Saputri, Legis Patya Putri, Hudaynu Perdana Priya Haresmita, Perdana Priya Pradani, Missya Putri Kurnia Priya Haresmita, Perdana Purnomo, Indra Putra, Bayu Karunia Putri, Fawwaz Aqila Putri, Hudaynu Patya Rachmalia Izzatul Mukhlishah, Neneng Rahmayanti, Wulan Desi Razkianita, Haulya Rizqullah, Rifqi Rosida, Naela Rosyidi, A. Sammanta, Rahula Vijja Saputra, Yoga Dwi Sasvania, Anisa Sugeng Riyanto Sulthanah Ahmad, Dzakiyyah Susi Rahayu Sutanti, Baiq Novia Rahmadita Tasya Kamila, Afifah Tunggal Arya Rezky, Noval Ulya, Tuhfatul Ussarwan, M Ihya Wardani, Arief Kusuma Wardani, Ray Haerul Zaneta, Nabila Destia Zanuba, Anisa