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Journal : Research Journal of Life Science

Identification of Plankton on Fish Pond of Oreochromis niloticus Infected by Viral Nervous Necrosis Uun Yanuhar; Muhammad Musa; Dyah Tri Rahayu; Diana Arfiati
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.761 KB) | DOI: 10.21776/ub.rjls.2016.003.02.6

Abstract

The purpose of study is to determine the type of plankton found in cultivation pond of nile tilapia infected by VNN. The method is to identify the plankton in the water pond and testing the VNN that infects nile tilapia related to plankton eaten using Polymerase Chain Reaction (PCR). VNN identification results on the nile tilapia digestive tract stomach and intestines as well as plankton found in the stomach and intestines indicate VNN positively. Plankton found in the digestive tract of fish is division of Chlorophyta, Bacillariophyta, phylum Rotifera and Crustacea. It indicates that nile tilapia infected by VNN both on the gastrointestinal tract organs, stomatch or intestines and plankton were found with positive VNN digestive duct, while plankton found in water pond is VNN negative. The VNN infection depends on the nature of specifications VNN on nile tilapia and the plankton in the nile tilapia digestive duct.
Effect of Cinnamaldehyde Addition on Zebrafish (Danio rerio) Eggs Against Streptococcus agalactiae Infection Asthervina Widyastami Puspitasari; Diana Arfiati; Shao-Yang Hu
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.334 KB) | DOI: 10.21776/ub.rjls.2017.004.03.5

Abstract

In this study, the effect of cinnamaldehyde (CA) towards Streptococcus agalactiae on zebrafish egg were investigated. To obtain the optimal concentration, CA and S. agalactiae were tested by short-term effects assay. Fresh zebrafish egg, CA and S. agalactiae were observed in different groups, single and mix treatment. The result showed that the larval survival rate (%) of single CA concentration (2.5 and 5 µg mL-1) were not significantly different compared to the control, with the value 94 ± 2.8% and 93 ± 3.3% respectively. Meanwhile, the single S. agalactiae concentration (109 and 1010 CFU mL-1) were significantly different in comparison to the control with the value 73 ± 2.1% and 6 ± 2.5%. Mix groups, larval survival rate (%) value were significantly different with the best combination value was 17 ± 4.7% at 5 µg mL-1 of CA and 109 CFU mL-1 of S. agalactiae. Even though CA may potential for zebrafish egg shelter against S. agalactiae infection. But a mixture (CA and S. agalactiae) treatments were not recommended for this study because the low value was shown compared to the single treatments. This possibility may occur because of the debris formation of two substances which leads to the poor environment and causes to low fish larval survival rate (%) value. For further study, the water quality assay is needed.
Determination on Yellow Fin Tuna Stock (Thunnus albacares) In South Java Sea Based on Genetic Variation by Restriction Fragment Length Polymorphism (RFLP) Method Feni Iranawati; Lazimatun Nazifah; Ledhyane Ika Harlyan; Syarifah Hikmah Julinda Sari; Diana Arfiati
Research Journal of Life Science Vol 3, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (721.238 KB) | DOI: 10.21776/ub.rjls.2016.003.01.2

Abstract

Indonesia is known as a country with the highest potential production of tuna, with total production more than six hundred thousand ton per year as value as 6.3 trillion IDR per year.However, Tuna fishery have several challenges, as can be identify by the declining in productivity and an average length fish had been caught, and also tuna fishing groundsaregetting farther. This apparently appears as a result of inaccurate determination of fish stock as the basic management unit of tuna fisheries, especially in yellow fin tuna (YFT). This study aims to define population stocks of YFTin South Java Sea based on genetic information. Samples were taken from three fish landing ports (MuaraBaru, Cilacap and Benoa). Restriction Fragment Length Polymorphism (RFLP) technique were applied for Cytochrome Oxidase I genes, using restriction enzymes;HaeIII, RsaI, BamHI, XhoI, and AluI. Genetic variation in populations were evaluated with POPGENE32 software. Result indicate that finer scale of stocks present YFT and should bemanaged differently for effective management purpose and to maintain sustainability of this species.
The Biological Aspects Comparison of Nila Tilapia (Oreochromis Niloticus) on The Eutrophic and Oligotrophic Reservoir from Indonesia Arfiati, Diana; Saputri, Nadya Agustarina; Styaningrum, Nimas; Widyastami, Asthervina; Dina, Karina Farkha; Lailiyah, Shofiyatul; Pratiwi, Rizky Kusma; Inayah, Zakiyyah Nur
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.1

Abstract

Comparison of the length, weight and condition factor of Nila Tilapia (Oreochromis niloticus) was studied on oligotrophic waters (Wonorejo Reservoir) conducted from July to August 2018 and eutrophic waters (Selorejo Reservoir) on February to March 2019. All of the reservoir are in the East Java, Indonesia. The total sample from Wonorejo Reservoir about 79 fish from 3 times sampling (every two weeks), but the fish Sample from Selorejo Reservoir are 35 from 4 times sampling every week. The Nila Tilapia from Wonorejo Reservoir are 15.3 ± 2.5 cm (length) and 74.6 ± 38.6 grams (weight) but fish from Selorejo are 17.8 ± 2.2 cm average length and 114 ± 35 grams for the weight. The condition factor of Nila Tilapia in both reservoirs was relatively the same, with an average value was 1.01. The Nila Tilapia caught from Selorejo Reservoir (eutrophic) is relatively heavier and bigger than in the Wonorejo Reservoir (oligotrophic) even though the hook size is smaller. Fish from the Selorejo Reservoir (eutrophic) is easier to get food so that the average fish is larger.
The Effect of Water Treatment Models to Reduce Lead (Pb) Level on Freshwater Snail Filopaludina javanica Arfiati, Diana; Syahid, Nur; Anwari, Zaki; Aminin, Aminin; Kusriani, Kusriani; Herawati, Endang Yuli; Puspitasari, Asthervina Widyastami
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.3

Abstract

Lead is a kind of non-essential heavy metals included in the metal causing environmental pollution with persistent properties that might harm the consumers. This study aimed to determine the best method for reducing the lead level on the freshwater snail Filopaludina javanica using three various water treatment models. Soaking water treatment (6h, 12h, 18h, 24h), flowing water treatment (6h, 12h, 18h, 24h), and refreshing water treatment (6h, 12h, 24h). The lead level assay in both samples used Atomic absorption spectroscopy (AAS), and the physical and chemical parameters were measured, such as temperature, pH, and Dissolved Oxygen (DO). The lowest value of lead content on freshwater’s soft body snail and water sample respectively were soaking water treatment at 6h (0.64 ± 0.02 mg L-1) and 24h (0.0045 ± 0.0015 mg L-1); flowing water treatment at 24h (0.04 ± 0.007 mg L-1) and 18h (0.0036 ± 0.0009 mg L-1) and; refreshing water treatment at 24h (0.150 ± 0.011) and 12h (0.007 ± 0.001), with control 0.072 ± 0.00 mg L-1 and 0.067 ± 0.00 mg L-1. Therefore, the most effective model to reduce the lead content was flowing water treatment within 24h in the freshwater soft body snail and 18h in the water sample.
Co-Authors Abdul Rahem Faqih Abdul Wafi Abimanyu Bayu Saputra Adita Ramadanti Afella Osa Agnes Khofiffah Agum Bayu Gumelar Agung Pramana Warih Marhendra Aida Sartimbul Aisyah, Fidhiyah Dita Dahria Aisyah, Jumrotul Alfarisi, Muhammad Asnin Alif Raditya Amirul Huda Alvateha, Dini Amin Leksono Aminin, Aminin Aminuddin Firdaus Andi Kurniawan Andi Masriah Aniek Masrevaniah Aniek Masrevaniah Anjasmara, Aang Setyawan Anna Catharina Sri Purna Suswati Anugerah, Putri Anwari, Zaki Apriliyanti, Fisma Josara Arning Wilujeng Ekawati Arning Wilujeng Ekawati Asus Maizar Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Athaillah Mursyid Athaillah Mursyid Audina I. Pratiwi Baghaz Dzulhamdhani Surya Putra, Renanda Bagyo Yanuwiadi Bambang Semedi Cahya, Muhamad Dwi Cakrawira Gundo Catur Budi Noviya Dewi Nur Setyorini Dian Vidiastuti, Dian Diana Putri Renitasari Dina, Karina Farkha Dini Alvateha Djaduk, Saddam Langkung Dyah Tri Rahayu Ekki Windi Endang Yuli Herawati Ertikasari, Nuning Evellin Dewi Lusiana Evellin Dewi Lusiana, Evellin Dewi Evi Veronica Faizal Kasim Feni Iranawati Fidhiyah Dita Dahria Aisyah Firdaus, Aminuddin Fresty Esmi Samber Gayatri, Alifia Gunawan Wibisono Guntur Guntur Hadi Nur Rohman Hadiana, Hadiana Hamami, Syeftyan Muhammad Ali Happy Nursyam Hartati Kartika Ningsih Heri Ariadi Herwati Umi Subarijanti Hu, Shao-Yang Inayah, Zakiyyah Nur Irawati Mei Widiastuti Irsyah Rahmi Jefri Permadi Karimah Karimah Khofiffah, Agnes Kurnianingsih, Asmiyati Kusriani Kusriani Kusuma, Wahyu Endra Lailiyah, Shofiatul Lazimatun Nazifah Ledhyane Ika Harlyan Lestariaji, Chandika Lini Murni Listiya Gita Lesmana Loka, Widi Ayom Maharani, Patricia Dita Maheno Sri Widodo, Maheno Sri Marsoedi Marsoedi Marsoedi Marsoedi Maulidiyah, Vika Mazaya, Amalia Febryane Adhani Miftahul Khair Kadim Miftahul Khair Kadim Mochammad Sholichin Mohammad Mahmudi Mohammad Qodri Fitra Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Nafar Amani Syams Muhklas Shah Winarno Mulyanto Mulyanto Mutia Nur Hayati Nadiro, Vina Nur Nanik Retno Buwono Neni Diah Kusumaning Arum Nia Kurniawan Nuddin Harahap Nunik Cokrowati Nunik Cokrowati Nur Maulida Safitri Nur Syahid, Nur Nurhabib, Asro Nurhalisa Nurhalisa Nurkhasanah, Anissa Aprilia Orchida, Kharisma Pane, Elya Putri Patresna, Laelatul Nisa Permanasari, Setya W. A. Prabandani, Alfurena Pratama Diffi Samuel Pratiwi, Rizky Kusma PURWANTI PURWANTI Puspitasari, Asthervina Widyastami Putra, Renanda Baghaz D.S. Putri Anugerah Rahardjo, Seto Sugianto Prabowo Rahmi Nurdiani Rahmi Valina Raisa Safara Renanda Baghaz Dzulhamdhani Surya Putra Renanda Baghaz Dzulhamdhani Surya Putra Renitasari, Diana Putri Ristianadewi, Priska Rizky Kusma Pratiwi Rizky Kusma Pratiwi Rizky Kusma Pratiwi Rukmini Rukmini Safara, Raisa Samuel, Pratama Diffi Saputri, Nadya Agustarina Sartika Tangguda Savira, Riza Sawiya Sawiya Septi Anitasari, Septi Setya Widi A. P. Shao-Yang Hu Shofiyatul lailiyah Shofiyatul Lailiyah Shofiyatul Lailiyah Slamat Slamat Soemarmo Soemarmo Soemarno Soemarno Sofiatul Sri Andayani Sri Andayani Sri Andayani Sri Dayuti Sri Intan Anggraini Sri Lestari Styaningrum, Nimas Suliswanto Suliswanto Supriatin, Febriyani Eka Syarifah Hikmah Julinda Sari Tangguda, Sartika Taufik Budhi Pramono Taufik Budhi Pramono Tinny D Kaunang TIWI NURJANNATI UTAMI Umi Zakiyah, Umi Valina, Rahmi Vina Nur Nadiro Widyastami, Asthervina Winarno, Muhklas Shah Yanuhar, Uun Yenny Risjani Yuni Kilawati Zakiyyah Nur Inayah Zakiyyah Nur Inayah