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All Journal Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Prosiding Seminar Biologi Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung The Journal of Experimental Life Sciences (JELS) Jurnal Pembangunan dan Alam Lestari Proceeding International Conference on Global Resource Conservation AGRIVITA, Journal of Agricultural Science Jurnal Pendidikan Biologi Indonesia Universa Medicina Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosaintifika: Journal of Biology & Biology Education Journal of Research in Instructional Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Kedokteran Hewan Indonesian Journal of Jamu
Sutiman B. Sumitro
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Veterinary Other

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Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Mubarakati, Nurul Jadid; Aulanni'am, Aulanni'am; Sumitro, Sutiman Bambang; Ciptadi, Gatot
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
Complex Compound with Transitional Metal of Akway Bark (Drimys piperita Hook f.) as Low Molecular Weight Scavenging Antioxidant: a Computational Study Hutasoit, Hostalige; Djoko, Dionysius Joseph; Sumitro, Sutiman B; Widjanarko, Simon Bambang
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.02

Abstract

Akway (Drimys piperita Hook f.) was known as an endogenous plant of Indonesia. This plant was investigated as free radical scavenger based on paramagnetic properties. This study aimed to identify the bioactive compound and the role of Fe metals ions to enhance the free radical scavenger capacity as Low molecular weight antioxidant (LWMA) of akway. This study was designed by ultra-performance liquid chromatography (UPLC) coupled with ultra-high-resolution time of flight-mass spectrometry detector (TOF-MS), docking (Pyrex and Discovery Studio 2016 Client) and pharmacokinetic properties prediction (SwissADME). UPLC-TOF-MS analysis showed that herbal akway bark has five molecular formula (C4H3N10O21-, C14H19N4O91-, C4H7N6O51-, C15H28NO31+, and C23H32NO71+). The docking was illustrated the complex binding akway formula with Fe and its role as a metal donor and metal acceptor. It also confirmed on pharmacokinetics properties prediction which molecular compounds of akway have aqueous solubility. It meant the herbal akway bark complexed to Fe could be a low molecular weight antioxidant and it could be escalated free radical scavenger capacity.
Co-Authors a, Aulanni'am A, Aulanniam A.A. Ketut Agung Cahyawan W Agus Hadian Rahim Ahmad Taufiq Amiruddin A Amiruddin Amiruddin Anis Artiyani Aristyani, Sherry Arman Sayuti Arman Sayuti Asri Pudjirahaju Atok Miftachul Hudha Aulanni'am, Aulanni'am Basuki B. Purnomo Basuki B. Purnomo Bayyinatul Muchtaromah Chomsa Dintasari Umi Baszary Dina Maulina Djoa, Dominikus Djago Djoko W. Soeatmadji Djoko, Dionysius Joseph Dody Novrial, Dody Dorta Simamora Dwinna Aliza Dwinna Aliza Edi Widjajanto Erdiansyah Rahmi Erdiansyah Rahmi Fatchiyah Fatah Fatchur Rohman Gatot Ciptadi Hutasoit, Hostalige Ibrohim Ibrohim Ignatius Riwanto, Ignatius Iwan Iwan Iwan Lalu A. Zaenuri Lestari, Sri Rarayu Listijani Suhargo, Listijani Loeki Enggar Fitri M. Aris A. Widodo M. Aris Widodo M. Aris Widodo M. Irsyad Nur Moch Sasmito Djati Mohamad Amin Mohammad Aris Mohammad Hidayat Muhammad Aris Widodo Muhammad Hambal Muhammad Hambal Mulyadi Adam Mulyadi Adam, Mulyadi Muslim Akmal Muslim Akmal Nashi Widodo Nur Hidayat Nurul Jadid Mubarokati Radita Intan Aisyah Pratiwi Rustidja , Sembiring, Rinawati SIMON BAMBANG WIDJANARKO Soetomo Soewarto Sri Rahayu Lestari Sri Wahjuningsih Sri Wahyuningsih Sri Widyarti Suhartojo Hardjopranjoto, Suhartojo Swandari Paramita Syafruddin s Syafruddin S T. Armansyah T. Armansyah Tongku Nizwan Siregar Warsito Warsito Zairin Noor