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Multiplikasi Tunas Tembakau Secara In Vitro Menggunakan Benzyl Amino Purine Dan Furfuryl Amino Purine Melalui Metode Thin Cell Layer Khozin, Mohammad Nur; Mona, Muhammad Dima Say; Dewanti, Parawita; Putri, Widya Kristyanti; Soeparjono, Sigit; Restanto, Didik Pudji
Al-Kauniyah: Jurnal Biologi Vol 18, No 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.40649

Abstract

Tembakau sebagai bahan baku pembuatan rokok mempunyai nilai ekonomi dan ekspor yang tinggi, namun permasalahan perbanyakan secara konvensional sering menghasilkan keturunan yang heterogen dan beberapa komoditas introduksi seringkali mengalami pertumbuhan yang tidak normal pada fase pembibitan sehingga pemenuhan kebutuhan bahan tanam yang seragam sering menjadi kendala. Kultur in vitro dapat menjadi alternatif dalam perbanyakan bahan tanam yang relatif seragam dan tahan terhadap perubahan lingkungan. Penggunaan zat pengatur tumbuh (ZPT) utamanya golongan sitokinin seperti benzyl amino purine (BAP) dan kinetin sangat mendukung pada multiplikasi tunas tembakau. Penelitian ini bertujuan untuk mengetahui konsentrasi BAP dan kinetin yang optimal pada multiplikasi tunas tembakau. Penelitian menggunakan rancangan acak lengkap (RAL) faktorial dengan faktor BAP (0, 2, 3, dan 4 ppm) dan faktor kinetin (0, 3, dan 4 ppm). Hasil penelitian menunjukkan interaksi BAP dan kinetin berpengaruh sangat nyata terhadap kedinian eksplan bertunas, jumlah tunas, dan daun dengan perlakuan terbaik. Konsentrasi BAP 3 ppm + kinetin 4 ppm yang menginduksi tunas pada 8,3 HST; jumlah tunas 81,3; dan jumlah daun 142,3 helai. Penggunaan BAP berpengaruh nyata terhadap kedinian eksplan berkalus dengan perlakuan terbaik pada konsentrasi 3 ppm BAP yang menginduksi kalus pada 10,78 HST. Kinetin berpengaruh sangat nyata terhadap kedinian eksplan bertunas dengan perlakuan terbaik P2M2 yaitu 4 ppm, yang menginduksi tunas pada 8,3 HST. Kesimpulannya perlakuan BAP 3 ppm + 4 ppm kinetin merupakan perlakuan terbaik.
Pemberdayaan Masyarakat Tani Desa Suling Wetan Kabupaten Bondowoso Dalam Upaya Penyediaan Bibit Nilam Secara Mandiri Ubaidillah, Mohammad; Setiyono, Setiyono; Slameto, Slameto; Hariyono, Kacung; Hartatik, Sri; Avivi, Sholeh; Dewanti, Parawita; Puspito, Agung Nugroho; Jalil, Abdul
Journal of Community Development Vol. 6 No. 1 (2025): August
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/comdev.v6i1.1447

Abstract

The majority of residents in Suling Wetan Village work as farmers and rely heavily on self-produced seeds due to the village’s remote location. One high-potential crop for marginal lands in the area is patchouli (Pogostemon cablin), a plant valued for its essential oil. However, the propagation of patchouli remains a significant challenge for local farmers due to limited knowledge and skills. This community engagement program aimed to enhance farmers’ capacities in patchouli propagation through a combination of awareness campaigns, technical workshops, hands-on training, direct field mentoring, and participatory monitoring and evaluation. Evaluation was conducted through direct observation and participant surveys. The results showed that 96% of participants gained a clear understanding of patchouli propagation techniques, and all participants acknowledged the importance of the program and expressed hope for its continuation. The activity successfully contributed to increasing farmers’ independence in sustainable patchouli seedling production.
THE EFFECT OF TDZ (Thidiazuron) ON THE FORMATION OF DIRECT SOMATIC EMBRYOGENESIS IN Phalaenopsis sp. ORCHID Adinda, Intan Dwi; Restanto, Didik Pudji; Dewanti, Parawita; Munandar, Denna Eriani; Hartatik, Sri; Prayoga, Mohammad Candra; Solikhah, Ummi
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.9795

Abstract

Orchids (Phalaenopsis sp.) are among the most valuable ornamental plants due to their wide range of flower colors and high commercial demand. However, conventional propagation methods are often inefficient for large-scale production, necessitating the optimization of tissue culture techniques for rapid and uniform clonal propagation. The use of appropriate plant growth regulators, particularly thidiazuron (TDZ), is crucial for enhancing somatic embryogenesis—a key pathway for orchid micropropagation. This study aimed to determine the optimal concentration of TDZ for inducing somatic embryogenesis in Phalaenopsis sp. through histological analysis and scanning electron microscopy (SEM) observations. A completely randomized design was employed using Murashige and Skoog (MS) medium supplemented with four TDZ concentrations (1, 3, 5, and 7 mg/L). Leaf explants of Phalaenopsis sp. served as the explant source. The results demonstrated that direct somatic embryogenesis successfully occurred from leaf explants across treatments. Among the tested concentrations, 5 mg/L TDZ produced the most effective response, resulting in the highest somatic embryo formation rate (32%) and the shortest time to embryo maturation (37 days after culture initiation). The embryos exhibited characteristic dark green coloration and a crumbly texture. These findings highlight the pivotal role of TDZ in promoting somatic embryogenesis in Phalaenopsis sp., providing a reliable protocol for efficient orchid propagation. The study contributes to the advancement of orchid biotechnology by offering histological and ultrastructural evidence that supports the optimization of clonal propagation systems for commercial and conservation purposes.
OPTIMASI PERTUMBUHAN PLANLET ANGGREK Dendrobium gabriella suryajaya MENGGUNAKAN MEDIA ALTERNATIF AB MIX DAN AIR KELAPA SECARA IN VITRO Dewanti, Parawita
Agrin Vol 27, No 2 (2023): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2023.27.2.743

Abstract

Tanaman anggrek merupakan salah satu tanaman yang sulit melakukan perkembangbiakan secara generatif, termasuk anggrek Dendrobium gabriella suryajaya sehingga diperlukan suatu cara agar tanaman anggrek dapat berkembang biak dalam waktu yang cepat dan terhindar dari patogen. Salah satu cara yang dapat dilakukan adalah dengan teknik kultur jaringan atau In Vitro dengan penambahan  suplemen organik yaitu air kelapa sebagai Zat Pengatur Tumbuh (ZPT). Secara umum, teknik kultur in vitro menggunakan bahan kimia untuk media kultur dan ZPT. Oleh karena itu, penggunaan media alternatif dan suplemen organik air kelapa diperlukan dalam penelitian ini untuk mengurangi pengggunaan bahan kimia. Penelitian ini bertujuan untuk mengetahui pengaruh air kelapa dan media alternatif AB Mix terbaik, serta interaksi keduanya terhadap pertumbuhan planlet anggrek Dendrobium gabriella suryajaya. Penelitian menggunakan metode Rancangan Acak Lengkap (RAL) secara faktorial dengan dua faktor perlakuan yaitu konsentrasi media kultur AB Mix dan konsentrasi ZPT air kelapa, di mana masing-masing faktor terdiri dari 3 taraf. Variabel yang diamati meliputi jumlah tunas, tinggi tunas, waktu pembentukan tunas, jumlah helai daun, jumlah akar, tinggi planlet, persentase planlet hidup, warna daun. Analisis data menggunakan Analysis of Variance (ANOVA) dan apabila hasil analisis berbeda nyata maka akan dilakukan uji lanjut DMRT dengan. Konsentrasi AB Mix terbaik adalah M3 (AB Mix 1500 ppm) yaitu pada jumlah tunas sebanyak 2, tinggi tunas sebesar 1,38, jumlah akar sebanyak 15, jumlah helai daun sebanyak 7, tinggi tanaman senilai 2,59 cm, dan jumlah Planlet hidup dengan persentase 100%. Serta Konsentrasi air kelapa terbaik adalah K4 (Air Kelapa 30%) yaitu pada tinggi planlet senilai 2,99 dan presentase planlet hidup sebesar 100%.
Multiplikasi Tunas Tembakau Secara In Vitro Menggunakan Benzyl Amino Purine Dan Furfuryl Amino Purine Melalui Metode Thin Cell Layer Khozin, Mohammad Nur; Mona, Muhammad Dima Say; Dewanti, Parawita; Putri, Widya Kristyanti; Soeparjono, Sigit; Restanto, Didik Pudji
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.40649

Abstract

Tembakau sebagai bahan baku pembuatan rokok mempunyai nilai ekonomi dan ekspor yang tinggi, namun permasalahan perbanyakan secara konvensional sering menghasilkan keturunan yang heterogen dan beberapa komoditas introduksi seringkali mengalami pertumbuhan yang tidak normal pada fase pembibitan sehingga pemenuhan kebutuhan bahan tanam yang seragam sering menjadi kendala. Kultur in vitro dapat menjadi alternatif dalam perbanyakan bahan tanam yang relatif seragam dan tahan terhadap perubahan lingkungan. Penggunaan zat pengatur tumbuh (ZPT) utamanya golongan sitokinin seperti benzyl amino purine (BAP) dan kinetin sangat mendukung pada multiplikasi tunas tembakau. Penelitian ini bertujuan untuk mengetahui konsentrasi BAP dan kinetin yang optimal pada multiplikasi tunas tembakau. Penelitian menggunakan rancangan acak lengkap (RAL) faktorial dengan faktor BAP (0, 2, 3, dan 4 ppm) dan faktor kinetin (0, 3, dan 4 ppm). Hasil penelitian menunjukkan interaksi BAP dan kinetin berpengaruh sangat nyata terhadap kedinian eksplan bertunas, jumlah tunas, dan daun dengan perlakuan terbaik. Konsentrasi BAP 3 ppm + kinetin 4 ppm yang menginduksi tunas pada 8,3 HST; jumlah tunas 81,3; dan jumlah daun 142,3 helai. Penggunaan BAP berpengaruh nyata terhadap kedinian eksplan berkalus dengan perlakuan terbaik pada konsentrasi 3 ppm BAP yang menginduksi kalus pada 10,78 HST. Kinetin berpengaruh sangat nyata terhadap kedinian eksplan bertunas dengan perlakuan terbaik P2M2 yaitu 4 ppm, yang menginduksi tunas pada 8,3 HST. Kesimpulannya perlakuan BAP 3 ppm + 4 ppm kinetin merupakan perlakuan terbaik.
Response of Rice Somatic Embryogenesis to Exogenous Melatonin About Its Role in Scavenging Reactive Oxygen Species Ubaidillah, Mohammad; Al Ayyubi, Nabila Nur Aisyah; Khofifa, Rendryana Aulia Nur; Dewanti, Parawita
AGRIVITA Journal of Agricultural Science Vol 46, No 1 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i1.4060

Abstract

The success rate of explant morphogenesis in plant breeding using tissue culture techniques is frequently plagued by browning due to the oxidation of phenolic compounds. The cumulated amount of reactive oxygen species (ROS) drives the oxidation of phenolic compounds. Melatonin is reported to take a part in modulating the regulation of antioxidant gene expression, reducing the accumulation of reactive oxygen species, and enhancing the efficacy of tissue culture. This study aims to determine the optimal melatonin concentration on the efficiency of plantlet regeneration and expression of the antioxidant resistance gene in rice callus. This study utilizes rice TN1, Gogo Niti II, Ketan Hitam, and Cigeulis cultivars. Melatonin at 0, 10, and 15 µM concentrations is supplemented in plantlet regeneration media. Rice antioxidant-related genes, Mn-SOD, Cu/ZnSOD, Cytosolic APX, CAT, GPOD, OsAPX, and OsCATA, expressed after melatonin supplementation. Melatonin concentration at 10 µM generates the highest expression of all tested genes in TN1 compared to other varieties. The cumulated amount of hydrogen peroxide (H2O2) shows that Melatonin has the potential to increase the proportion of plant regeneration in Cigeulis (90.48%) and Ketan Hitam (91.67%) varieties with a concentration of 10 µM and in TN1 (94.44%) and Gogo Niti II (80%) at a concentration of 15 µM.
Direct Organogenesis of Different Explants of Aceh Patchouli (Pogostemon cablin Benth.) with Several BAP Concentrations Prayoga, Mohammad Candra; Soeparjono, Sigit; Dewanti, Parawita; Handoyo, Tri; Hardjo, Popy Hartatie; Restanto, Didik Pudji
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.12839

Abstract

The patchouli plant (Pogostemon cablin Benth.) is a tropical herbaceous plant that produces essential oil. One of the problems is that the production is not yet optimal. Fulfillment of superior seedlings can help increase patchouli productivity. Conventional patchouli propagation through stem cuttings is ineffective and takes longer. Patchouli propagation can be done using a tissue culture approach via direct organogenesis to produce seedlings quickly and efficiently. Effective patchouli propagation methods and successful acclimatization are very important to research to support the propagation and breeding of patchouli plants. The aim of this research was to determine the best of BAP concentration in direct organogenesis of leaf and stem explants. The research design used a completely randomized series of hormone BAP, it has 5 levels, namely 0 mg/L (as control), 0.25 mg/L, 0.50 mg/L, 0.75 mg/L, and 1.0 mg/L. The explants used were the leaves and stems of Aceh patchouli. Plantlets are acclimatized in compost media and covering treatment. Based on the results of observations, the best BAP concentration is 0.25 mg/L with the initial observation parameters of the early emergence of shoots, number of shoots, and length of shoots on leaf explants were 10 daps, 35.33 shoots, and 2.83 cm respectively. The use of leaf explants showed a better response compared to stem explants. Patchouli plantlets were successfully acclimatized and can adapt to the ex vitro environment using the covering method. Successful patchouli propagation and high acclimatization can help produce effective patchouli seeds.
The Effect of Nutritioning Interval on Automatic Drip Hydroponic System on Growth and Production of Three Varieties of Lettuce (Lactuca sativa L.) Ristiyana, Suci; Fanata, Wahyu Indra Duwi; Saputra, Tri Wahyu; Purnamasari, Ika; Dewanti, Parawita; Taufik, Rahadian Falqi
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 1 (2025): February 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i1.146-154

Abstract

Lettuce (Lactuca sativa L.) is a leaf vegetable that has a high level of consumer demand and commercial value and can be cultivated using a combination of hydroponic substrate and drip irrigation methods. This research aims to determine the effect of the time interval for providing nutrients on the growth and harvest results of three lettuce plant varieties. Plants were cultivated on cocopeat and husk charcoal media (ratio 1:3) and arranged using a Randomized Block Design (RAK) consisting of two treatments. The first treatment is the nutritional interval which consists of an interval of 2 hours with a discharge of 17 ml (I1), 3 hours with a discharge of 25 ml (I2), and 4 hours using a discharge of 34 ml (I3). The second treatment is the plant variety which consists of the Grand Rapids variety (V1), the Green Coral variety (V2), and the Red Coral variety (V3). The data is analyzed using analysis of variance and if the results obtained are significantly different then a DMRT test will be carried out. The research results showed that the Green Coral lettuce variety was better than the Grand Rapids and Red Coral varieties. This is shown by the results with the highest and best values for the observation variables of number of leaves, plant fresh weight, and chlorophyll content. The nutritional interval which consists of an interval of 2, 4, and 6 hours gave results that were inversely proportional to the variety treatment, that is, they were not significantly different in all observed variables. Keywords: lettuce plants, plant varieties, nutritional interval, substrate hydroponics
Analysis of the Application of Vitamin B1 on the Response of Salinity Stress Resistance in Several Varieties of Rice (Oryza Sativa L.) Ratnasari, Tri; Handoyo, Tri; Dewanti, Parawita; Restanto, Didik Pudji
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.6324

Abstract

One of the most common stresses in rice cultivation is salinity. Rice plants stressed by salinity exhibit changes such as yellowing leaves, drying tips, and chlorosis. The efforts made by the government and farmers so far include implementing cultivation scheduling techniques, planting patterns, and using stress-resistant varieties, as well as improving soil to increase water-holding capacity through lime application. Each of these efforts comes with its own risks. Another approach to enhance the growth and yield of rice plants is the application of vitamins. Providing vitamins can stimulate the growth of plant organs, as they play a crucial role in the growth process by acting as catalysts for metabolism. Research has indicated that vitamin B1 can significantly promote plant growth under stressful conditions. This study aims to investigate the positive effects of various concentrations of vitamin B1 on the growth and yield of rice plants while also reducing salinity stress. The method used involved planting three varieties of rice—IR-46, Inpari-32, and Pokkali—in planting buckets using the TABELA system. Vitamin B1 was applied at concentrations of 0, 5, and 10 mM during the peak vegetative phase, with salinity stress of 6 dS/m introduced one day after vitamin application. The plants were maintained under salinity stress conditions until harvest, during which morphological and phytochemical analyses were conducted. Morphological analysis included measurements of plant height, number of tillers, number of productive tillers, number of grains per panicle, and percentage of healthy grains. Biochemical parameters measured included total chlorophyll and electrolyte leakage analysis. The results indicate that vitamin B1 can effectively reduce stress in plants affected by salinity.
Somatic Embryogenesis of Dendrobium lasianthera X Dendrobium antennatum with the Addition of BA and NAA Sasmita, Heni Dwi; Dewanti, Parawita; Alfian, Firdha Narulita
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 2 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (939.71 KB) | DOI: 10.24831/jai.v50i2.39715

Abstract

Somatik embriogenesis merupakan teknik perbanyakan kultur in vitro melalui proses pembelahan sel yang berasal dari bagian tanaman untuk membentuk embrio menjadi tanaman baru. Perbanyakan tersebut didukung oleh penambahan BA dan NAA dalam media untuk memicu terbentuknya kalus. Tujuan penelitian ini untuk memperoleh konsentrasi kombinasi BA dan NAA yang terbaik dalam proses induksi kalus tanaman anggrek Dendrobium Hibrida (Dendrobium lasiantera x Dendrobium antennatum) melalui metode somatik embriogenesis. Penelitian ini dilakukan di Laboratorium Center for Development of Advance Sciences and Technology (CDAST) Universitas Jember Jawa Timur dari bulan Maret-September 2021. Rancangan penelitian yang digunakan yaitu rancangan acak lengkap dengan dua faktor yaitu faktor BA 4 taraf, 0, 0.025, 0.05, dan 0.1 mg L-1 dan faktor NAA 3 taraf; 1, 2, dan 3 mg L-1, dengan menggunakan tiga ulangan. Hasil penelitian menunjukkan waktu terbentuknya kalus anggrek Dendrobium Hibrida paling cepat pada perlakuan dengan konsentrasi BA 0.025 mg L-1 dan NAA 3 mg L-1 dengan presentase tertinggi terbentuknya kalus embriogenik yaitu sebesar 67%. Hasil penelitian menunjukkan bahwa penambahan BA 0.025 mg L-1 dan NAA 3 mg L-1 merupakan hasil terbaik pada variabel waktu terbentuknya kalus dan presentase kalus embriogenik.
Co-Authors . Usmadi Abdul jalil Adinda, Intan Dwi Al Ayyubi, Nabila Nur Aisyah Alfian, Firdha Narulita Alifah Farida Sa’adah Alifatul Aqidah Arya Wiranegara Azmi Saleh Bagus Tripama Bambang Soegiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Banun Kusumawardani Budi Kristanto Budi Kriswanto Cesha Ananda Putri Desi Kartika Sutrisno Dwi Setyati Dwika Nano Hariyanto Erma Sulistyaningsih Fanata, Wahyu Indra Duwi Firdausi, Intan Firdha Narulita Alfian Firdha Narulita Alfian Firdha Narulita Alfian Firdha Narulita Alfian Hakim, Mohammad Sulton Harsanti, Restiani Sih Hidayat Hidayat Hidayat Ika Purnamasari Indraloka, Aldy Bahaduri Intan Dwi Ambarwati Inyana Dwi Agustien Kacung Hariyono Khofifa, Rendryana Aulia Nur Khozin, Mohammad Nur Laela Endah Rahmadhani Laily Ilham Widuri Laily Ilman Widuri, Laily Ilman Lenny Widjayanthi Magfiroh, Illia Seldon Maisaro, Maisaro Maryam, Safira Arikha Mohammad Ali Mudhor Mona, Muhammad Dima Say Muhammad Hazmi Munandar, Denna Eriani Nadiya, Nisma Riyadh Nafisah Iqmatullah Nina Oktaria Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Olandino Tome Francisco Dorosario de Sousa Popy Hartatie Hardjo Pralampita, Pulong Wijang Prayoga, Mohammad Candra Purnama Okviandari Purnama Okviandari, Purnama Puspito, Agung Nugroho Putri, Cesha Ananda Putri, Widya Kristyanti Raden Soedradjad Rahmadhani, Laela Endah Raisah Bani Ratnasari, Tri Restanto, Didik Restanto, Didik Pudji Ristiyana, Suci Rizky Harikurniawan Rudi Joelijanto Sasmita, Heni Dwi Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Siti Kamalia Siti Nurul Afidah Slameto Slameto Soegiharto, Bambang Sri Hartatik Sulistiyono Syafira Fatihatul Husna Taufik, Rahadian Falqi Tri Agus Siswoyo Tri Agus Siswoyo TRI HANDOYO Tri Wahyu Saputra Ubaidillah, Mohammad Ummi Solikhah, Ummi Veronenci Yuliarbi Farlisa Wachju Subchan Wahyu Indra Duwi Fanata Wardatus Sholeha Widuri, Laily Ilham Widya Kristiyanti Putri Wulan Nursyiam Ningtiyas Wulanjari, Distiana Yani Corvianindya Rahayu