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Evaluation Of Spirulina, Nannochloropsis, and Chlorella Micro-algae Growth in Palm Oil Mill Effluent (POME) Medium with Variation of Medium Types and Time Adding Nutrient Muhamad Zaini Mahdi; Yasinta Nikita Titisari; H. Hadiyanto; Marcelinus Christwardana
Journal of Bioresources and Environmental Sciences Vol 1, No 1 (2022): April 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14239

Abstract

POME is a kind of liquid waste produced by the crude palm oil industry. POME was not treated adequately, resulting in an issue for the environment owing to excessive levels of COD and BOD. Algae is a kind of bio-absorbent that may neutralize contaminants in liquid waste. Microalgae need carbon, nitrogen, and phosphorus-containing ingredients to flourish. These nutrients are necessary for photosynthetic carbon sources to be converted into biomass. POME includes a high concentration of C, N, and P; hence this research aims to investigate the potential of POME as a medium for the development of algae such as Spirulina, Nannochloropsis, and Chlorella. The experiment was carried out by varying the nutrients, water type, and time of nutrient feeding. Urea and sodium bicarbonate were the nutrition. Preparing the medium, culturing the microalgae, assessing biomass, counting the algae cells, and creating a calibration curve were the procedures in the experiment. The findings revealed that POME is the best medium for microalgae, that Spirulina grows better in POME than Chlorella and Nannochloropsis, and that providing nutrients every 2 days was better than introducing nutrients at the beginning and without adding nutrients.
Utilization of chicken eggshell and chitosan as coagulants for microplastic removal from aquatic system Aaron Nathanael Liemin; Monica Kezia Sembiring; H Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 2, No 1 (2023): April 2023
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2023.16478

Abstract

Smaller size plastics known as microplastics are harmful for marine biota as well as humans. One of the common methods for the removal of microplastics from water is coagulation. Chicken eggshells and chitosan are two natural ingredients which contain useful content for the coagulation process. Therefore, this paper describes the effect of concentration of eggshells and chitosan as coagulants in the separation of microplastic from water using RSM (Response Surface Methodology). The effect of tannic acid concentration as coagulation aid was also analyzed in this paper. The coagulants were varied with concentration of 1.5-8.5 g/100 mL and tannic acid concentration was 3-17 mg/100 mL. The result of this study showed coagulation with the highest efficiency occurred when 8.5 g/100 mL of chicken eggshell coagulant (89.14%) and 10 g/100 mL chitosan coagulant (75.67%) was added. Optimum operation occurred after the addition of coagulant aid in the amount of 7 mg/100 mL with chicken eggshell and 15 mg/100 mL with chitosan. 
Cultivation of Microalgae Spirulina platensis in Palm Oil Mill Effluent (POME) Media with Variations of POME Concentration and Nutrient Composition Fitria Yuli Anggita Sari; I Made Aditya Suryajaya; Marcelinus Christwardana; H. Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.15052

Abstract

Indonesia and Malaysia are recognized as the world's leading producers of palm oil. Along with the growth of the palm oil industry in Indonesia, the amount of Palm Oil Mill Effluent has increased. Palm Oil Mill Effluent (POME) is a liquid byproduct of the palm oil production process. POME has been treated using aerobic and anaerobic ponds to lower Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD) levels, but it still includes nutrients like as C,N,P that are beneficial to the development of microalgae. On this study, Spirulina platensis was grown in POME medium with 20%, 40%, and 60% V. concentrations. Every two days, the nutrients Urea, NaHCO3, and TSP were administered. Seven days of aeration and 24-hour lights are used throughout the cultivation phase. The results indicate that POME with a 20 % concentration is the optimal medium for plant growth. Add 25 ppm Urea, 50 ppm TSP, and 200 ppm NaHCO3 for the optimal nutritional composition. At the same treatment on various medium, the highest growth rate of Spirulina platensis is determined to be µ = 0.128% per day, with an optical density of 0.648. Carbon reductions range from 83.03 % to 84.10 %, while Nitrogen savings range from 78 % to 79.55 % when POME is used as a growing medium. This study also shown that the C, N, and P concentrations of POME fall by 93 to 98 %, 99 to 99.5 %, and 92 to 97 %, respectively.
Microplastic Identification in Fisheries Commodities in Sayung Waters, Demak Fuad Muhammad; H. Hadiyanto; Abdurrafi Alwan
Journal of Bioresources and Environmental Sciences Vol 1, No 1 (2022): April 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14226

Abstract

Each year plastic production worldwide has increased. Approximately 10% of the production of plastic will lead to the sea. This plastic is degraded to be a small particle size < 5mm called microplastic. The river is the main route of entry of plastic from land to the sea. Sayung River is a river that has the potential to be contaminated with microplastics around which it is used as a location for the cultivation of various fisheries commodities including Pernaviridis, Penaeus monodon, and Lates calcarifer. The study aims to determine the number and type of microplastic in Penaeus monodon, Pernaviridis, and Lates calcarifer. Samples were taken from three different stations in the coverage area. Microplastic abundance analysis by isolating microplastic on each sample. Isolation samples of Pernaviridis, Penaeus monodon, and Lates calcarifer are done by dissolving the sample in a solution of 10% KOH was allowed for 24 hours at a temperature of 60 oC and observed under a binocular microscope. Founded types of microplastic are fiber, fragments, pellets, and films on Pernaviridis, Penaeus monodon, and Lates calcarifer. Pernaviridis found on many types of films, in Penaeusmonodon are the most prevalent types of fragments and the Lates calcarifer most common types of fiber. Pellet type is the least kind found in Pernaviridis and Penaeus monodon. Based on the age when taken, Penaeus monodon is the commodity that has the most potential to be contaminated with microplastic while based on the number of particles found in Lates calcarifer is the commodity with the most potential to be contaminated with microplastic.
Optimization of light intensity and color temperature in the cultivation of Chlorella vulgaris culture using the Surface Response Method Marcelinus Christwardana; H. Hadiyanto; Wahyu Zuli Pratiwi
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14410

Abstract

Microalgae have an important role as a source of biomass in producing energy. One type of microalgae that has the potential to be developed is Chlorella vulgaris. Several factors that affect the growth and biomass production of Chlorella vulgaris microalgae are color temperature and light intensity because they play an important role in the photosynthesis process. This study aims to influence the effect of light and color temperature and optimize these parameters using Response Surface Methodology (RSM). Two independent variables were varied: light intensity 200, 400, 600, 800, 1000 lux and color temperature 3000, 4000, 5000, and 6000 K. The results showed that the average value of Chlorella vulgaris growth was higher along with higher light intensity. At a color temperature of 4000 K, the highest biomass yield and the most negligible biomass production were found at 6000 K. At a color temperature of 4000 K, it is feasible to apply it as an alternative lighting source in the production of Chlorella vulgaris. The combination of light intensity and color temperature shows that the specific growth rate and doubling time have opposite trends where high values produce low values and vice versa. Growth in dark conditions, the specific growth rate was 0.0026 day-1, and the optimal light intensity at 600 lux treatment. ANOVA evaluation showed that color temperature greatly affected growth. Based on the optimization, the optimal specific growth rate of 00751day-1 with the conditions of light intensity and color temperature of 556 lux and 4152 K, respectively.  
Proximate Analysis and Hedonic Test on Dried Noodle with The Addition of Spirulina platensis Microalgae as A High Protein Food Marcelinus Christwardana; Aniek Sri Handayani; Evita Febriyanti; H. Hadiyanto; Angela Nitia Nefasa
Journal of Bioresources and Environmental Sciences Vol 2, No 1 (2023): April 2023
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2023.17445

Abstract

The study focuses on malnutrition. Dietary intake influences malnutrition. Indonesians choose dry noodles due of their portability. Dry noodles often include more carbohydrates than protein; consequently, the addition of Spirulina platensis may increase their protein level while de-creasing their carbohydrate load. This research evaluated the levels of protein, fat, water, and ash in dry noodles before and after supplementation with spirulina platensis. 1% and 2% of the fixed variable weight of Spirulina platensis were used. Included in analyses are protein, fat, water, and ash. Variation I (1%) results protein of 15.79%, whereas Variation II (2%) has 28.21%. Variation I (1%) have 13.07% moisture, whereas Variation II (2%) has 13,15%. Variation I (1%) contain 0.233% fat compared to variation II (2%) of 0.223%. Variation I (1%) had 1.445% ash content, whereas Variation II (2%), contained 2% ash. The nutritional value of Grade II or dried noodles with Spirulina platensis conforms to Indonesian National Standard 8217-2015. Spirulina platensis increases the pro-tein content of dry noodles when added. According to SNI 8217-2015 grade II, the addition of spir-ulina to dried noodles improved their protein, water, ash, and fat content. The nutritious content of dried noodles was improved by the inclusion of Spirulina platensis.
Preliminary Observation of Biogas Production from a Mixture of Cattle Manure and Bagasse Residue in Different Composition Variations H Hadiyanto; Figa Muhammad Octafalahanda; Jihan Nabila; Andono Kusuma Jati; Marcelinus Christwardana; Kusmiyati Kusmiyati; Adian Khoironi
International Journal of Renewable Energy Development Vol 12, No 2 (2023): March 2023
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.2023.52446

Abstract

The need of renewable energy is paramount important as it is expected to replace fossil energy. One of renewable energy commonly used for rural area is biomass-based energy. Biogas is a biomass-based energy where organic materials are converted to methane gas via anaerobic digestion process. The limitations of mono-feedstock biogas are instability digestion process, low yield biogas produced and require readjusting C/N ratio, therefore co-digestion process was proposed to overcome these problems. This study aims to investigate the feasibility of anaerobic co-digestion of a mixture of cattle manure and bagasse residue in different weight ratio combinations. Biogas was generated by anaerobic digestion using a mixed substrate composed of a combination of weight ratios of bagasse:cattle manure (1:5, 1:2, 1:1, and 3:1). The kinetic analysis was evaluated by fitting Gompertz and Logistic model to experimental data of cumulative biogas. The result showed that the combination of 1:5 ratio of bagasse waste to cattle manure obtained the best biogas yield with cumulative biogas at 31,000 mL. The kinetic model of Gompertz and Logistic were able to predict the maximum cumulative biogas at ratio of 1:5 (cattle: bagasse) at 31,157.66 mL and 30,112.12 mL, respectively. The other predictions of kinetic parameters were maximum biogas production rate (Rm)= 1,720.45 mL/day and 1,652.31 mL/day for Gompertz and Logistic model, respectively. Lag periods were obtained at 2.403 day and 2.612 day for Gompertz and Logistic model, respectively. The potential power generation of 338.71 Watt has been estimated from biogas. This research has proven a positive feasibility of co-digestion of two feed-stocks (cattle manure and bagasse) for biogas production.
Potency of Microalgae as Biodiesel Source in Indonesia Hadiyanto Hadiyanto; Widayat Widayat; Andri Cahyo Kumoro
International Journal of Renewable Energy Development Vol 1, No 1 (2012): February 2012
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.1.1.23-27

Abstract

Within 20 years, Indonesia should find another energy alternative to substitutecurrent fossil oil. Current use of renewable energy is only 5% and need to be improved up to 17%of our energy mix program. Even though, most of the area in Indonesia is covered by sea, howeverthe utilization of microalgae as biofuel production is still limited. The biodiesel from currentsources (Jatropha, palm oil, and sorghum) is still not able to cover all the needs if the fossil oilcannot be explored anymore. In this paper, the potency of microalgae in Indonesia was analysed asthe new potential of energy (biodiesel) sources.
Bioethanol Production from Iles-Iles (Amorphopallus campanulatus) Flour by Fermentation using Zymomonas mobilis Kusmiyati Kusmiyati; H Hadiyanto; Indah Kusumadewi
International Journal of Renewable Energy Development Vol 5, No 1 (2016): February 2016
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.5.1.9-14

Abstract

Due to the depletion of fossil oil sources, Indonesia attempts to search new source of bioenergy including bioethanol. One of this sources is Iles-iles tubers (Amorphophallus campanulatus), which is abundantly available in Java Indonesia. The carbohydrate content in Iles-Iles tuber flour was 77% and it can be converted to ethanol by three consecutive steps methods consist of liquefaction-saccharification using α and β-amylase, respectively and then followed by fermentation by using Z. mobilis. The objective of this research was to convert the Iles-iles flour to bioethanol by fermentation process with Z.mobilis. The ethanol production process was studied at various starch concentration 15-30% g/L, Z. mobilis concentration (10-40%) and pH fermentation of (4-6). The result showed that the yield of bioethanol (10.33%) was the highest at 25% starch concentration and 25% of Z.mobilis concentration. The optimum conditions was found at 4.5, 30°C, 10%, 120 h for pH, temperature, Z. mobilis concentration and fermentation time, respectively  at which  ACT tuber flour produced a maximum ethanol of 10.33 % v/v.Article History: Received November 12nd 2015; Received in revised form January 25th 2016; Accepted January 29th 2016; Available onlineHow to Cite This Article: Kusmiyati , Hadiyanto,H  and Kusumadewi, I (2016). Bioethanol Production from Iles-Iles (Amorphopallus campanulatus) Flour by Fermentation using Zymomonas mobilis. Int. Journal of Renewable Energy Development, 9(1), 9-14http://dx.doi.org/10.14710/ijred.5.1.9-14 
Batch and Fed-Batch Fermentation System on Ethanol Production from Whey using Kluyveromyces marxianus H Hadiyanto; D. Ariyanti; A.P. Aini; D.S. Pinundi
International Journal of Renewable Energy Development Vol 2, No 3 (2013): October 2013
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.2.3.127-131

Abstract

Nowadays reserve of fossil fuel has gradually depleted. This condition forces many researchers to  find energy alternatives which is renewable and sustainable in the future. Ethanol derived from cheese industrial waste (whey) using fermentation process can be a new perspective in order to secure both energy and environment. The aim of this study was  to compare the operation modes (batch and fed-batch) of fermentation system on ethanol production from whey using Kluyveromyces marxianus. The result showed that the fermentation process for ethanol production by fed-batch system was higher at some point of parameters compared with batch system. Growth rate and ethanol yield (YP/S) of fed-batch fermentation were 0.122/h and 0.21 gP/gS respectively; growth rate and ethanol yield (YP/S) of batch fermentation were 0.107/h, and 0.12 g ethanol/g substrate, respectively. Based on the data of biomass and ethanol concentrations, the fermentation process for ethanol production by fed-batch system were higher at some point of parameters compared to batch system. Periodic substrate addition performed on fed-batch system leads the yeast growth in low substrate concentrations and consequently  increasing their activity and ethanol productivity. 
Co-Authors - Hermawan - Purwanto . Widayat A Abdullah A.P. Aini A.P. Aini Aaron Nathanael Liemin Abdurrafi Alwan Achmad Chalid Afif Alfajrin Achmad Roesyadi Achmad Roesyadi Adhi, M. A. Adi Wibowo Adian Khoironi Afriza Ni&#039;matus Sa&#039;adah Afshar, Mohammad Ali Agus Suhatman Agus Suprihanto Ahmadi, Mohammad Hossein Aini, Apsari Puspita Ainun Nurusy Syahida Aji Prasetyaningrum Al-Baari, Ahmad N Al-Baarri, Ahmad Ni’matullah Albertus Djoko Lesmono Alhuyi Nazari, Mohammad Alkian, Ilham Amalia Farhati Anak Agung Istri Sri Wiadnyani Andono Kusuma Jati Andri C. K. Andri Cahyo Kumoro Andri Cahyo Kumoro Andry Anggoro Arahim Angela Nitia Nefasa Angela Nitia Nefasa Anh Tuan Hoang Aniek Sri Handayani Aniek Sri Handayani Anisa Widia Utami Annisa Sila Puspita Apsari Puspita A. Apsari Puspita Aini Arief Budiman Arief Budiman Arief Budiman Arief Budiman Arif Harimukti Arif Setiawan Arifati Munfarida Aris Bagus Pradana, Aris Bagus Asep Yoyo Wardaya Asri Cahaya Hati Athanasia Amanda Septevani, Athanasia Amanda Avido Yuliestyan Avido Yuliestyan Awaluddin Hidayat Ramli Inaku Azafilmi Hakiim Aziz Jihadian Barid B. Budhijanto Baihaqi, Rifqi Ahmad Bambang Cahyono Bambang Yulianto Barid, Aziz Jihadian Bariroh, Tahyatul Bayu, Handoko Bidi, Mokhtar Budhijanto, B. Cahyadi Cahyadi Cahyadi Cahyadi Cantika Aulia Salsabila Catur Edi Widodo Chandra Puspita Sari D A Sari D. Ariyanti D. Ariyanti D.S. Pinundi D.S. Pinundi Danny Soetrisnanto Dedi Khairunas Desiyantri Siti P. Dessy Agustina Sari Dessy Ariyanti Dessy Ariyanti Dewi Selvia Fardhyanti Didi Dwi Anggoro Dionisius Dicky Davidoff Dyah Ari Wulandari Dyah Hesti Wardhani Dyan Pratiwi E Hugeng Wandono Ebrahimi, Morteza Eko Hidayanto Eko Wahjono, Tri Endang Kusdiyantini Eudia Christina Wulandari Evita Febriyanti Faradies Arija Febio Dalanta Ferdinandus Baskoro Hari Sapto Waluyo Fianda Revina Widyastuti Figa Muhammad Octafalahanda Fitria Yuli Anggita Sari Fitria Yuli Anggita Sari Fitriyanto, Bobby Rachmat Fitriyanto, Bobby Rachmat Franz, Nicholas Fuad Muhammad Fuad Muhammad Fuad Muhammad Ganang Dwi Hartanto Ganang Dwi Hartanto Ghasempour, Roghayeh Hakim, Muhammad Fahmi Hamdani Abdulgani, Hamdani Hamid Hamid Haniif Prasetiawan Hantoro Satriadi Hapsoro Bagus Wicaksono Hargono Hargono Haris Nu'man Aulia Hashfi Hawali Abdul Matin Hati, Asri Cahaya Hemawan, Ferry Hendra Naldi Henna Rya Abdurachim Henna Rya Sunoko Henna Rya Sunoko Heri Cahyono Heri Sutanto Hermawan Hermawan Hermawan Hermawan Hidayat Ramli Inaku, Awaluddin Hoang, Anh Tuan I Made Aditya Suryajaya I Made Aditya Suryajaya Iis Nurhasanah Imam Novrizal Aji Indah Kusumadewi Indah Kusumadewi, Indah Indradewa, Rhian Indro Sumantri Indro Sumatri Irawan Arif Budianto Irfan Ali, Irfan J. Jamari Jaka Windarta Jihan Nabila Joni Safaat Adiansyah Kamandjaja, Vimala Kevin Aprilio Kusmiyati Kusmiyati Kusmiyati Kusmiyati Le, Tri Hieu Luqman Buchori M Christwardana M Christwardana M M A Nur M. Barbosa M. Djaeni M.M. Azimatun Nur M.M. Azimatun Nur, M.M. Azimatun Marcelinus Christwardana Marcelinus Christwardana Marcelinus Christwardana Marcelinus Christwardana Marcelinus Christwardana Marcelinus Christwardana Marcelinus Christwardana Marcelinus Christwardana Mardiyah Sari Dewi Maryono Maryono Matin, Hashfi Hawali Abdul Mega Anggraeni Mega Anggraeni Meiny Suzery Miftah Aqidatul Izzah Minh Tuan Pham Mochamad Arief Budihardjo Moh. Djaeni Moh. Djaeni Mohammad Alhuyi Nazari Mohammad Hossein Ahmadi Monica Kezia Sembiring Morteza Ebrahimi Muhamad Imaduddin Muhamad Maulana Azimatun Nur Muhamad Zaini Mahdi Muhammad Fadhil Muhammad Fajar Purnama Muhammad Helmi Muhammad Nasyarudin Iqbal Muhammad Zaini Mahdi Munifatul Izzati Nailawati Prastiya Ningrum Nainggolan, Irwan Firmanto Naseri, Ali Ndaru Okvitarini Ngadi, Noorzita Ngadi, Norzita Nicholas Franz Noer Abyor Handayani Noor Fachrizal Nurahman, Ghiyats Hafizh P Purwanto P. Purwanto Paryanto, Paryanto Permata, Ariyo Nurachman Satiya Perwana, Putu Yuri Aristya Pham, Minh Tuan Pham, Van Viet Pratama, Wahyu Diski Pratiwi, Wahyu Zuli Pribadyo Pribadyo Prima Astuti Handayani Primadi Gayuh Laksono Putro Priyasta, Dwidharma Purwanto - Purwanto Purwanto Purwanto Purwanto Purwono Purwono Putri, Sylvia Rahmi R. Rochmadi R. Wijffels Rachmad Ardhianto Ganjar Samudro Rahma, Dhyeta Ulzana Zizi Ratna Dewi Kusumaningtyas Ratnawati Ratnawati Reistu Widiastutik Retno Wulandari Rifqi Ahmad Baihaqi Riky Yonas Riky Yonas Rini Budi Hastuti Rita Dwi Ratnani Riza Saadiah, Riza Rizal Isnanto, R RM Bagus Irawan Rochmadi, R. Rocky Adiguna Roghayeh Ghasempour Rufaida Nur Rostika Rya Abdurachim, Henna S Subandriyo S Sumarno S. Suherman Sa'adah, Fatkhiyatus Sabda Wahyu Nugroho Sakina Tunissa Anarki Santi, Narulita Satrio Kuntolaksono Septiawan, Reza Sestri Bela Pratiwi Setia Budi Sasongko Setiawan, M. Yasep Silviana Silviana Singgih Wibowo Sinuhaji, Tubagus Rayyan Fitra Siswo Sumardiono Siti Jamilatun Siti Jamilatun Sri Puji Lestari Sri Sumiyati Sri Widodo Agung Sueda Sri Widodo Agung Suedy Sudarno Sudarno Suherman Suherman Suherman, Suherman Sulardjaka Sulardjaka Sulardjaka, S. Sumarno Sumarno Susanto, Edy Suseno Darsono Sussatrio, Hardito Syafrudin Syafrudin Syahidah, Aisyah Sabrina Nurul Thomas Triadi Putranto, Thomas Triadi Titik Nurmawati Tony Suryo Utomo Tri Eko Wahjono Tri Hieu Le Tri Retnaningsih Tri Retnaningsih Soeprobowati Tubagus Rayyan Fitra Sinuhaji Tutuk Djoko Kusworo Uray Irzandi Uray Irzandi Valentino, Andreas Alvaro Van Viet Pham Vimala Kamandjaja Vita Efelina W. Wihermanto Wahyu Cesar, Wahyu Wahyu Zuli Pratiwi Wais Alqurni Wicaksono, Vinsensius Priyo Widayat Widayat Widayat Widayat Widayat Widiyanto Widiyanto Widya FATRIASARI Wilujeng, Dinanti Putrisia Windarta, Djaka Wiwie Chaeruni Yasinta Nikita Titisari Yasinta Nikita Titisari Yoyon Wahyono Yudariansyah, Hadi Yulian Mara Alkusma YULIANI, YENI Yulis Hindarwati Zakaria, Zainul Akmar Zin Min Tun