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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Zuriat Jurnal Natur Indonesia Jurnal Agrista Jurnal Ilmiah Aplikasi Isotop Radiasi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Jurnal Penelitian Tanaman Industri Buletin Plasma Nutfah AGRIVITA, Journal of Agricultural Science Jurnal Agro Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL BIOLOGI INDONESIA JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Terapan Jurnal Penelitian Pertanian Tanaman Pangan ANNALES BOGORIENSES AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Entomologi Indonesia Menara Perkebunan Floribunda Jurnal Penelitian Tanaman Industri (Littri) Jurnal Pusat Inovasi Masyarakat Makara Journal of Science Annales Bogorienses
HAJRIAL ASWIDINNOOR
Departement Of Agronomy And Horticulture, Faculty Of Agriculture, IPB University (Bogor Agricultural University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, INDONESIA
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Journal : BERITA BIOLOGI

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PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 [Segregation of hpt Gene by PCR Analysis and its Expression in Transgenic Rice Population Overexpressing HD-Zip oshox6 Gene] Mulyaningsih, Enung Sri; Aswidinnoor, Hajrial; Sopandie, Didy; OuwerkerV, Pieter BF; Slamet Loedin, Inez Hortense
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

Abstract

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.
PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 Enung Sri Mulyaningsih; Hajrial Aswidinnoor; Didy Sopandie; Pieter BF OuwerkerV; Inez Hortense Slamet Loedin
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

Abstract

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.
Co-Authors , Rusdiansyah , Santoso , Supartopo , Susilawati , Suwarno , Tasliah . Jumanto . NURHAIMI-HARIS . Soedarsono . SUBRONTO . Sudarsono . SUWARNO A. Dinar Ambarwati A. Hairmansis A. Hairmansis Abdul Mollah S. JAYA Abdullah, Buang Agus PURWANTARA Agus Purwito Agus Rachmat Agus Rachmat AHMAD JUNAEDI AHMAD RIDUAN Ahmad Rifqi Fauzi Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati ALEX HARTANA ALEX HARTANA ALEX HARTANA Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amris Makmur Angelita P. Lestari Angelita Puji Lestari Angelita Puji Lestari Angelita Puji Lestari ANGELITA PUJI LESTARI Antonius Suwanto Aris Hairmansis ASEP SAEFUDDIN Asep Setiawan Asmini Budiani Ayub Darmanto Azrai, Muhammad Azrai, Muhammad S Azri Kusuma Dewi Azri Kusuma Dewi Bambang S. Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Bambang Suprihatno Buang Abdullah Buang Abdullah Buang Abdullah Budi Marwoto Budi Tjahjono Danang Aria Nugroho Daniel Happy Putra Desi Anugra Safitri Desta Wirnas Dewi Indriyani Roslim Dewi Sukma Dewi, Iswari S. Didy Sopandie Dini Nurdiani DINI NURDIANI Djoko Santoso Djoko Santoso Djoko Santoso Djoko Santoso DONATA S PANDIN Dwi Asmono DWI HAPSORO Dwi Hapsoro Dwi Hapsoro Dwinita W. Utami Dwinita Wikan Utami Dwinita Wikan Utami Dyah Kusuma Anggraini E. Suryaningsih Edi Guhardja Edi GUHARJA Edi Santosa Endrizal Endrizal ; Enung Sri Mulyaningsih Enung Sri Mulyaningsih Eri Sofiari Faqih Udin Fitrianingrum Kurniawati, Fitrianingrum G A WATTIMENA G. A. Wattimena G. A. Wattimena Gale Ginting Gale Ginting Gale Ginting GEDE SUASTIKA GEDE WIJANA Ghulamhdi, Munif Ghulammahdi, Munif Gustav A Wattimena Gustav A. Wattimena Helen Hetharie Herman, Muhamad Herman, Muhammad Hermanasari, Rini Hermanu Triwidodo HIASINTA FJ MOTULO Ida H. Somantri Ida H. Somantri Ida Hanarida Ida Hanarida Ida Hanarida Soemantri IDA HANARIDA SOMANTRI Indrastuti A. Rumanti Indrastuti A. Rumanti Inez H.S. Loeddin Suharsono Inez Hortense Slamet Loedin Inez Hortense Slamet Loedin Iskandar Lubis Iswari S. Dewi Iswari S. Dewi J. M. Tutupary JAJAH KOSWARA Jayaningsih, Elvita Dwi Joko Prasetiyono Jumanto Jumanto Jumanto Jumanto, Jumanto Kurniawan Rudi Trijatmiko Kurniawan Rudi Trijatmiko, Kurniawan Rudi Lestari, Angelita Puji Limbongan, Yusuf La’lang Lina Torizo Lollie Agustina P. Putri M A Chozin M. Herman M. Herman Maggy T Suhartono MAGGY T. SUHARTONO Maggy Thenawidjaya S Maggy Thenawidjaya S. Maggy THENAWIDJAYA-SUHARTONO Maria Swastika Mariana Susilowati Mariana Susilowati, Mariana Masdiar Bustamam Maulidiya, Sherly Eka MEITY S-SINAGA Mejaya, Made Jana Memen Surahman Miftahudin . Muhamad Herman Muhammad Achmad Chozin Muhammad Azrai Muhammad Herman Muhammad Herman MUHAMMAD HERMAN Muhammad Herman Muhammad Syukur Mukelar Amir Munif Ghulamahdi Nesti F Sianipar Nesti F SIANIPAR Nindita, Anggi Nugraha, Yudhistira NURITA TORUAN-MATHIUS Nurita Toruan-Mathius Nurita Toruan-Mathius, OuwerkerV, Pieter BF Pabendon, Marcia B Pieter B.F. Ouwerkerk Pieter BF OuwerkerV Purwoko, B. S. Rafiatul Rahmah Rafiatul Rahmah Ramadhan, Fitrah REFLINUR REFLINUR Rini Hermanasari Rini Hermanasari Roberdi, Roberdi S Roedhy Poerwanto Roedy Poerwanto Roedy Poerwanto Rumanti, Indrastuti A. Rumanti, Indrastuti A. RUSMILAH SUSENO Rusmilah Suseno S. M. Sumaraow S. M. Sumaraw Saptowo J. Pardal Sarsidi Sastrosumarjo Satoto, Satoto Satriyas Ilyas Satya Nugroho Satya Nugroho Setiawan, Asep S Sherly Rahayu Sherly Rahayu Sholeh Avivi Sientje Mandang Sumaraw SIENTJE MANDANG SUMARAW Sinaga, Parlin H. Sintho Wahyuning Ardie Siska Indriajaya Apriyani Slamet Loedin, Inez Hortense Soaloon Sinaga Soaloon Sinaga Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sri H Hidayat Sri H. Hidayat Sri H. Hidayat Sri Hendrastuti Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudirman Yahya SUGIONO MOELJOPAWIRO SUGIONO MOELJOPAWIRO Sugiono Moeljopawiro Sugiyanta , Sumaraow, S. M. Sumaraw, S M Suryaningsih, E. Sutrisno, Sutrisno S Suwarno ,, Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno, Suwarno Suwarno, Suwarno Swastika, Maria Swastika, Maria Syaifullah Rahim Syarifah Iis Aisyah TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tika Anisa Padar Wati Tintin Suhartini TRI HASTINI Tri Herdiyanti, Tri Tri J. Santoso Tri Joko Santoso Tri Joko Santoso Tri Joko Santoso Trias Sitaresmi Trikoesoemaningtyas Umi Salamah Untung Susanto Untung Susanto Utut Suharsono Virk S. Parminder Vitaliano Lopena Wage Ratna Rohaeni Wening Enggarini Wening Enggarini Willy Bayuardi Suwarno Yudhistira Nugraha Yulidar Yulidar Yullianida , Yuniarti Yuniarti Yusniwati Yusniwati Yusuf L. Limbongan Z LALU