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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Zuriat Jurnal Natur Indonesia Jurnal Agrista Jurnal Ilmiah Aplikasi Isotop Radiasi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Jurnal Penelitian Tanaman Industri Buletin Plasma Nutfah AGRIVITA, Journal of Agricultural Science Jurnal Agro Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL BIOLOGI INDONESIA JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Terapan Jurnal Penelitian Pertanian Tanaman Pangan ANNALES BOGORIENSES AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Entomologi Indonesia Menara Perkebunan Floribunda Jurnal Penelitian Tanaman Industri (Littri) Jurnal Pusat Inovasi Masyarakat Makara Journal of Science Annales Bogorienses
HAJRIAL ASWIDINNOOR
Departement Of Agronomy And Horticulture, Faculty Of Agriculture, IPB University (Bogor Agricultural University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, INDONESIA
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Journal : JURNAL BIOLOGI INDONESIA

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Kajian Pendahuluan: Perpindahan Gen dari Tanaman Kentang Transgenik Katahdin RB ke Tanaman Kentang Non Transgenik Ambarwati, A. Dinar; Herman, M.; Purwito, Agus rifcb@indo.net.id; Sofiari, Eri; Aswidinnoor, hajrial
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3114

Abstract

ABSTRACTPreliminary study: Gene transfer from transgenic potato Katahdin RB to non transgenicpotato. One of the concerns associated with the release of transgenic crops, is the possibilityof the gene flow from transgenic crops to neighboring crops of the same species or to relatedspecies. In plants, gene flow is a routine process occur through the natural hybridization. Theopportunity for gene flow occur depends principally on two factors, the degree of sexualcompatibility between donor and recipient species, and the physical distance between thetwo. The experiment was conducted to determine whether the gene flow from transgenicpotato Katahdin RB to non transgenic was occurred, based on selection using a 50 mg/lkanamycin, and to estimate gene flow mediated by natural hybridization at different isolationdistances. Preliminary result indicated that a rapid and simple method using MS0 liquid mediawith kanamycin 50 mg/l was effective for screening the seeds. There was a gene flow fromtransgenic potato Katahdin RB to non transgenic, based on a rapid and simple selectionmethod using 50 mg/l of kanamycin as selectable marker. The isolation distance used in thestudy were 0.8, 1.6, 2.4, 3.2, 4.0, 4.8, 5.6, 6.4, 7.2, 8.0, 8.8, 9.6, 10.4, and 11.2 m from the row oftransgenic potato Katahdin RB. The gene flow through natural hybridization at a isolationdistances of (0.8 - 1.6 m), (2.4 – 4 m), and (4.8 – 6.4 m) from transgenic to non transgenic plantswere 13.78, 10.92, and 3.82%, respectively. At a distance of 7.2 – 8 m, the frequency of gene flowwas declined to 0%. The frequency of gene flow from transgenic potatoes to non transgenicpotatoes markedly decreased by increasing the isolation distance, and was negligible at 7.2 m.Key words : natural hybridization, transgenic potato RB, kanamycin selection
KAJIAN PENDAHULUAN: PERPINDAHAN GEN DARI TANAMAN KENTANG TRANSGENIK KATAHDIN RB KE TANAMAN KENTANG NON TRANSGENIK Ambarwati, A. Dinar; Herman, M.; Purwito, Agus rifcb@indo.net.id; Sofiari, Eri; Aswidinnoor, hajrial
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3114

Abstract

ABSTRACTPreliminary study: Gene transfer from transgenic potato Katahdin RB to non transgenicpotato. One of the concerns associated with the release of transgenic crops, is the possibilityof the gene flow from transgenic crops to neighboring crops of the same species or to relatedspecies. In plants, gene flow is a routine process occur through the natural hybridization. Theopportunity for gene flow occur depends principally on two factors, the degree of sexualcompatibility between donor and recipient species, and the physical distance between thetwo. The experiment was conducted to determine whether the gene flow from transgenicpotato Katahdin RB to non transgenic was occurred, based on selection using a 50 mg/lkanamycin, and to estimate gene flow mediated by natural hybridization at different isolationdistances. Preliminary result indicated that a rapid and simple method using MS0 liquid mediawith kanamycin 50 mg/l was effective for screening the seeds. There was a gene flow fromtransgenic potato Katahdin RB to non transgenic, based on a rapid and simple selectionmethod using 50 mg/l of kanamycin as selectable marker. The isolation distance used in thestudy were 0.8, 1.6, 2.4, 3.2, 4.0, 4.8, 5.6, 6.4, 7.2, 8.0, 8.8, 9.6, 10.4, and 11.2 m from the row oftransgenic potato Katahdin RB. The gene flow through natural hybridization at a isolationdistances of (0.8 - 1.6 m), (2.4 ? 4 m), and (4.8 ? 6.4 m) from transgenic to non transgenic plantswere 13.78, 10.92, and 3.82%, respectively. At a distance of 7.2 ? 8 m, the frequency of gene flowwas declined to 0%. The frequency of gene flow from transgenic potatoes to non transgenicpotatoes markedly decreased by increasing the isolation distance, and was negligible at 7.2 m.Key words : natural hybridization, transgenic potato RB, kanamycin selection
Co-Authors , Rusdiansyah , Santoso , Supartopo , Susilawati , Suwarno , Tasliah . Jumanto . NURHAIMI-HARIS . Soedarsono . SUBRONTO . Sudarsono . SUWARNO A. Dinar Ambarwati A. Hairmansis A. Hairmansis Abdul Mollah S. JAYA Abdullah, Buang Agus PURWANTARA Agus Purwito Agus Rachmat Agus Rachmat AHMAD JUNAEDI AHMAD RIDUAN Ahmad Rifqi Fauzi Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati ALEX HARTANA ALEX HARTANA ALEX HARTANA Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amris Makmur Angelita P. Lestari Angelita Puji Lestari Angelita Puji Lestari Angelita Puji Lestari ANGELITA PUJI LESTARI Antonius Suwanto Aris Hairmansis ASEP SAEFUDDIN Asep Setiawan Asmini Budiani Ayub Darmanto Azrai, Muhammad Azrai, Muhammad S Azri Kusuma Dewi Azri Kusuma Dewi Bambang S. Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Bambang Suprihatno Buang Abdullah Buang Abdullah Buang Abdullah Budi Marwoto Budi Tjahjono Danang Aria Nugroho Daniel Happy Putra Desi Anugra Safitri Desta Wirnas Dewi Indriyani Roslim Dewi Sukma Dewi, Iswari S. Didy Sopandie DINI NURDIANI Dini Nurdiani Djoko Santoso Djoko Santoso Djoko Santoso Djoko Santoso DONATA S PANDIN Dwi Asmono DWI HAPSORO Dwi Hapsoro Dwi Hapsoro Dwinita W. Utami Dwinita Wikan Utami Dwinita Wikan Utami Dyah Kusuma Anggraini E. Suryaningsih Edi Guhardja Edi GUHARJA Edi Santosa Endrizal Endrizal ; Enung Sri Mulyaningsih Enung Sri Mulyaningsih Eri Sofiari Faqih Udin Fitrianingrum Kurniawati, Fitrianingrum G A WATTIMENA G. A. Wattimena G. A. Wattimena Gale Ginting Gale Ginting Gale Ginting GEDE SUASTIKA GEDE WIJANA Ghulamhdi, Munif Ghulammahdi, Munif Gustav A Wattimena Gustav A. Wattimena Helen Hetharie Herman, Muhamad Herman, Muhammad Hermanasari, Rini Hermanu Triwidodo HIASINTA FJ MOTULO Ida H. Somantri Ida H. Somantri Ida Hanarida Ida Hanarida Ida Hanarida Soemantri IDA HANARIDA SOMANTRI Indrastuti A. Rumanti Indrastuti A. Rumanti Inez H.S. Loeddin Suharsono Inez Hortense Slamet Loedin Inez Hortense Slamet Loedin Iskandar Lubis Iswari S. Dewi Iswari S. Dewi J. M. Tutupary JAJAH KOSWARA Jayaningsih, Elvita Dwi Joko Prasetiyono Jumanto Jumanto Jumanto Jumanto, Jumanto Kurniawan Rudi Trijatmiko Kurniawan Rudi Trijatmiko, Kurniawan Rudi Lestari, Angelita Puji Limbongan, Yusuf La’lang Lina Torizo Lollie Agustina P. Putri M A Chozin M. Herman M. Herman Maggy T Suhartono MAGGY T. SUHARTONO Maggy Thenawidjaya S Maggy Thenawidjaya S. Maggy THENAWIDJAYA-SUHARTONO Maria Swastika Mariana Susilowati Mariana Susilowati, Mariana Masdiar Bustamam Maulidiya, Sherly Eka MEITY S-SINAGA Mejaya, Made Jana Memen Surahman Miftahudin . Muhamad Herman Muhammad Achmad Chozin Muhammad Azrai Muhammad Herman MUHAMMAD HERMAN Muhammad Herman Muhammad Herman Muhammad Syukur Mukelar Amir Munif Ghulamahdi Nesti F Sianipar Nesti F SIANIPAR Nindita, Anggi Nugraha, Yudhistira NURITA TORUAN-MATHIUS Nurita Toruan-Mathius Nurita Toruan-Mathius, OuwerkerV, Pieter BF Pabendon, Marcia B Pieter B.F. Ouwerkerk Pieter BF OuwerkerV Purwoko, B. S. Rafiatul Rahmah Rafiatul Rahmah Ramadhan, Fitrah REFLINUR REFLINUR Rini Hermanasari Rini Hermanasari Roberdi, Roberdi S Roedhy Poerwanto Roedy Poerwanto Roedy Poerwanto Rumanti, Indrastuti A. Rumanti, Indrastuti A. RUSMILAH SUSENO Rusmilah Suseno S. M. Sumaraow S. M. Sumaraw Saptowo J. Pardal Sarsidi Sastrosumarjo Satoto, Satoto Satriyas Ilyas Satya Nugroho Satya Nugroho Setiawan, Asep S Sherly Rahayu Sherly Rahayu Sholeh Avivi SIENTJE MANDANG SUMARAW Sientje Mandang Sumaraw Sinaga, Parlin H. Sintho Wahyuning Ardie Siska Indriajaya Apriyani Slamet Loedin, Inez Hortense Soaloon Sinaga Soaloon Sinaga Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sri H Hidayat Sri H. Hidayat Sri H. Hidayat Sri Hendrastuti Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudirman Yahya SUGIONO MOELJOPAWIRO Sugiono Moeljopawiro SUGIONO MOELJOPAWIRO Sugiyanta , Sumaraow, S. M. Sumaraw, S M Suryaningsih, E. Sutrisno, Sutrisno S Suwarno ,, Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno, Suwarno Suwarno, Suwarno Swastika, Maria Swastika, Maria Syaifullah Rahim Syarifah Iis Aisyah TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tika Anisa Padar Wati Tintin Suhartini TRI HASTINI Tri Herdiyanti, Tri Tri J. Santoso Tri Joko Santoso Tri Joko Santoso Tri Joko Santoso Trias Sitaresmi Trikoesoemaningtyas Umi Salamah Untung Susanto Untung Susanto Utut Suharsono Virk S. Parminder Vitaliano Lopena Wage Ratna Rohaeni Wening Enggarini Wening Enggarini Willy Bayuardi Suwarno Yudhistira Nugraha Yulidar Yulidar Yullianida , Yuniarti Yuniarti Yusniwati Yusniwati Yusuf L. Limbongan Z LALU