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AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER KAPANG ENDOFIT DAUN TANAMAN BAWANG DAYAK (ELEUTHERINE AMERICANA (AUBL.) MERR.) SECARA IN VITRO Kumala, Shirly; Gabriel, Gabriel
Jurnal Farmasi Indonesia Vol 11, No 2 (2019)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v11i2.673

Abstract

Bawang dayak is a medicine plant which has been known potential as an antioxidant. To isolate potential secondary metabolites as antioxidant needs many bawang dayak plants to be extracted. Utilization of endophytic microbes as a source of secondary metabolites will make the production of natural medicine raw materials is more efficient and environmentally friendly. This research was aimed to investigate secondary metabolites of endophytic fungi from bawang dayak leaves as antioxidant substance. The method of this research was a direct seed inoculating technique on the surface of Potato Dextrose Agar directly. Identification of fungi morphology by using macroscopic (visual) and microscopic (slide culture) method. The results of endophytic isolation of this research got four fungi isolates. Isolate is fermented with shake method, then it is extracted with ethyl acetate. The extract is continued for Antioxidant activity assessment used DPPH method. Secondary metabolite ethyl acetate extract of four isolates had a potention as antioxidant. Highest antioxidant activity was showed by ethyl acetate extract from d.IEP.4.2 isolate has IC50 at 82,9750 µg/mL which has been classified as strong antioxidant.
Faktor-Faktor yang Berhubungan dengan Kejadian Infeksi Saluran Pernafasan Akut (ISPA) pada Balita Di Daerah Pesisir Kota Sibolga Tahun 2020 Pasaribu, Rita Kristina; Santosa, Heru; Kumala, Shirly; Nurmaini, Nurmaini; Hasan, Delina
Syntax Idea Vol 3 No 6 (2021): Syntax Idea
Publisher : Ridwan Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-idea.v3i6.1232

Abstract

ISPA (Acute Respiratory Tract Infection) is a transmitted respiratory tract disease caused by environmental and human factors. Balita (below five year-old children) is very vulnerable to it. It was caused by virus or bacteria. The symptoms are high fever, followed by pain in throat, difficult to swallow, flu, and coughing with or without phlegm transmitted through patients' droplet (Riskesdas,2018). Revealed that, nationally, the prevalence of ISPA in balita was 12.8%, 8. 7% in North Sumatera, and 57% in Sibolga. The objective of the research was to analyze some factors which were correlated with the incidence of ISPA in balita at the coastal area of Sibolga . The data were gathered by using questionnaires. The research used cross sectional design. The samples were 265 (Isaac and Michael tables). The data were analyzed by using univariate analysis, bivariate analysis, and multivariate analysis with multiple logistic regression tests. From the samples, it was found that 144 of them (54.3%) were affected by ISPA and 121 of them (45. 7%) were not. The result of univariate analysis showed that 141 respondents (53.2%) had good requirement for their floors, 146 of them (55.1%) for walls, 70 of them (26.4%) for air ventilation, 257 of them (97%) for room temperature, 225 of them (84.9%) for lighting, 233 of them (87.9%) for moisture, 181 of them (68. 3%) for population density. From the factor of children, it was found that there were 131 boys (49.4%), 188 of the balita (70.9%) were 6- 35 months old, 217 of them (81.9%) were not BBLR, 206 of them (77.7%) had bad nutrition, 160 of them (60.4%) got vitamin A, 139 of them (52.5%) got immunization completion, 177 of them (66.8%) were breastfed with ASI (breast milk), 202 of them (76. 2%) smoked, 40 of them (15.1 %) used wood as fuel, and 27 of them (10.2%) used mosquito coils The result of bivariate analysis showed that 8 (eight) independent variables which were correlated were smoking (p-value=0. 003), using mosquito coils (p-value=0.026), floors (p-value=0.033), walls (p-value=0.002), population density (p-value=0.001), nutritional status (pvalue=0.001), air ventilation (p-value=0.012), Vitamin A(p-value=0.024), and complete immunization (p-value=0.035). The result of multivariate analysis showed that the variables of smoking, condition of house walls, nutritional status, and immunization completion caused the incidence of ISPA in balita at the coastal area of Sibolga (75.8%). It is recommended that prevent ISPA by increasing nutritional status, ASI, visiting posyandu, and no smoking inside their houses.
n-Butanolic fraction of endofitic fungi of Buah Makasar increases apoptotic effect of doxorubicin on MCF-7 cells Kumala, Shirly
Indonesian Journal of Pharmacy Vol 20 No 1, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp42-47

Abstract

Makassar fruit, Brucea javanica (L.) Merr., showed chemopreventive activity. Secondary metabolites come from B. javanica fruit, brucatol and bruceantine, induced cell differentiation and apoptosis on Leukemia cell, while quassinoid and its derivates acted as antitumor promoter. Butanolic fraction of supernatan of endofitic fungi 1.2.11 isolate fermentation which isolated from B. javanica fruit showed cytotoxycity toward several cancer cells. This fraction has been predicted contain secondary metabolites from B. javanica and has been identified as Bruceosin and Canthin-6-one derivates. Butanolic fraction (FB) of supernatan from endofitic fungi 1.3.11 isolate fermentation is predicted for having similiar cytotoxycity as active as 1.2.11 isolate. This research is aimed to explore cytotoxycity potention and apoptosis induction of BF from 1.3.11 isolate and combination effect of BFdoxorubicinon MCF-7 breast cancer cell.Synergism of BF-doxorubicin combination detect from cell viability inhibition and apoptosis induction on MCF-7, a breast cancer cell lines which shows resistancy toward doxorubicin. Cell viability on single treatment of FB and doxorubicin and its combination were carried out by MTT assay to determine IC50 and combination index (CI). Apoptosis induction of FB, doxorubicin and its combination were carried out by ethidium bromideacridine orange DNA staining.n- Butanolic fraction and doxorubicin showed cell viability inhibition on MCF-7 cell with IC50 48 μg/mL dan 148 nM, respectively. Both of FB and doxorubicin showed apoptosis induction on IC50. Combination of FBdoxorubicin showed synergism and increased apoptosis induction on MCF-7cell.Key words: Brucea javanica, endofitic fungi, MCF-7 cell, synergism, doxorubicin.
ANALISIS FAKTOR-FAKTOR YANG MEMPENGARUHI OUTCOME TERAPI PASIEN PNEUMONIA DI RUMAH SAKIT UMUM PUSAT FATMAWATI JAKARTA Setiadi, Feri; Kumala, Shirly; Utami R, Hesty; Subhan, Ahmad
Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Vol. 5 No. 3 (2019)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.673 KB) | DOI: 10.22487/htj.v5i3.127

Abstract

Berdasarkan data Riset Kesehatan Dasar (Riskesdas)2018 prevalensi pneumonia di jakarta 2.3 %. Pneumoni mempunyai case fatality rate (CFR) yang tinggi yaitu 7.6% sehingga beresiko mempunyai outcome terapi yang tidak baik. Oleh karena itu penelitian ini bertujuan menganalisis faktor-faktor yang mempengaruhi outcome terapi pasien pneumonia di RSUP Fatmawati. Penelitian ini dilakukan secara retrospektif dengan metode cross sectional pada pasien pneumonia (hospital acquired pneumonia/HAP dan community-acquired pneumonia/CAP) yang memenuhi kriteria inklusi selama periode januari-september 2018. Penelitian ini dilakukan dengan pengkajian data rekam medis pasien. Ada dua jenis outcome terapi yang digunakan yaitu pasien pulang/sembuh dan pasien meninggal. Analisis faktor yang mempengaruhi outcome dilakukan dengan uji chi square menggunakan SPSS. Selama penelitian didapatkan 40 pasien memenuhi kriteria inklusi terdiri dari 22 HAP dan 18 CAP. Hasil umur pasien (65.0%) berada pada kelompok usia 17-65 tahun. Lebih banyak pasien laki-laki (57.5%) dibandingkan pasien perempuan (42.5%). Sebagian besar pasien (37.5%) di rawat selama 8-14 hari. Penggunaan antibiotik (35.0%) terbanyak digunakan antibiotik ceftriaxone golongan sefalosporin. Proporsi pasien dengan outcome sembuh/pulang sebanyak (52.5%) dan pasien meninggal ( 47.5%). Hasil analisis statistik menunjukkan faktor yang signifikan mempengaruhi outcome adalah penyakit penyerta (P=0.022) Adapun faktor yang tidak ada hubungan bermakna terhadap outcome pasien pneumonia yaitu: usia pasien, lama perawatan dan kerasionalan antibiotik.
Pengaruh Edukasi Farmasis terhadap Outcome Terapi Pasien TB Paru Fase Intensif di RSUP Persahabatan Periode Maret-Juli 2015 Reza Agung Sriwijaya; Shirly Kumala; Sesilia A. Keban
Jurnal Penelitian Sains Vol 20, No 3 (2018)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.976 KB) | DOI: 10.56064/jps.v20i3.514

Abstract

The background knowledge of this research is based on the amount of patients’ pulmonary tubercu-losis who get failed doing their therapy and recur in having it after doing the treatment at RSUP Persahaba-tan.The objective of this research is to decrease the amount of patients get failed doing the therapy and recur at RSUP Persahabatan. The method of the research is Quasi-experimental with Nonrandomized Control Group Pretest-Postest Design. Furthermore, the technical sampling used is voluntaryby two apparent groups, 54 pa-tients as control group and 54 patients as experiment group.The instrument of this research are pre-test,posttest and Likert Questionair Model which is used to measure the knowledge and compliance toward the outcome of the therapy before and after it meets a good validity and reability test with standard α = 0,05. The conclusion is education pharmacy can increase knowledge and compliance with TB patients so that the outcomes of therapy against Pulmonary TB patients at RSUP Persahabatan is improved.
Faktor-Faktor yang Berhubungan dengan Kejadian Infeksi Saluran Pernafasan Akut (ISPA) pada Balita Di Daerah Pesisir Kota Sibolga Tahun 2020 Rita Kristina Pasaribu; Heru Santosa; Shirly Kumala; Nurmaini Nurmaini; Delina Hasan
Syntax Idea Vol 3 No 6 (2021): Syntax Idea
Publisher : Ridwan Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/syntax-idea.v3i6.1232

Abstract

ISPA (Acute Respiratory Tract Infection) is a transmitted respiratory tract disease caused by environmental and human factors. Balita (below five year-old children) is very vulnerable to it. It was caused by virus or bacteria. The symptoms are high fever, followed by pain in throat, difficult to swallow, flu, and coughing with or without phlegm transmitted through patients' droplet (Riskesdas,2018). Revealed that, nationally, the prevalence of ISPA in balita was 12.8%, 8. 7% in North Sumatera, and 57% in Sibolga. The objective of the research was to analyze some factors which were correlated with the incidence of ISPA in balita at the coastal area of Sibolga . The data were gathered by using questionnaires. The research used cross sectional design. The samples were 265 (Isaac and Michael tables). The data were analyzed by using univariate analysis, bivariate analysis, and multivariate analysis with multiple logistic regression tests. From the samples, it was found that 144 of them (54.3%) were affected by ISPA and 121 of them (45. 7%) were not. The result of univariate analysis showed that 141 respondents (53.2%) had good requirement for their floors, 146 of them (55.1%) for walls, 70 of them (26.4%) for air ventilation, 257 of them (97%) for room temperature, 225 of them (84.9%) for lighting, 233 of them (87.9%) for moisture, 181 of them (68. 3%) for population density. From the factor of children, it was found that there were 131 boys (49.4%), 188 of the balita (70.9%) were 6- 35 months old, 217 of them (81.9%) were not BBLR, 206 of them (77.7%) had bad nutrition, 160 of them (60.4%) got vitamin A, 139 of them (52.5%) got immunization completion, 177 of them (66.8%) were breastfed with ASI (breast milk), 202 of them (76. 2%) smoked, 40 of them (15.1 %) used wood as fuel, and 27 of them (10.2%) used mosquito coils The result of bivariate analysis showed that 8 (eight) independent variables which were correlated were smoking (p-value=0. 003), using mosquito coils (p-value=0.026), floors (p-value=0.033), walls (p-value=0.002), population density (p-value=0.001), nutritional status (pvalue=0.001), air ventilation (p-value=0.012), Vitamin A(p-value=0.024), and complete immunization (p-value=0.035). The result of multivariate analysis showed that the variables of smoking, condition of house walls, nutritional status, and immunization completion caused the incidence of ISPA in balita at the coastal area of Sibolga (75.8%). It is recommended that prevent ISPA by increasing nutritional status, ASI, visiting posyandu, and no smoking inside their houses.
Evaluasi Pelayanan Pemantauan Terapi Obat di Rumah Sakit X Tangerang Febbyasi Megawaty; Shirly Kumala; Sesilia Andriani Keban
JURNAL ILMU KEFARMASIAN INDONESIA Vol 18 No 1 (2020): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.474 KB) | DOI: 10.35814/jifi.v18i1.783

Abstract

Therapeutic Drug Monitoring (TDM) is a clinical practice to increase the effectiveness of therapy and minimize the risk of adverse drug reactions (ADR). The purpose of this study is to describe the implementation of TDM, and whether it is in accordance with the Regulation of the Minister of Health of the Republic of Indonesia (Permenkes) Number 72 of 2016 concerning pharmaceutical service standards at hospitals and the evaluation of TDM services conducted by pharmacists on inpatients. This research covers a descriptive study with a collection of retrospective data from TDM documents of inpatients during the period of January–May 2016 at X Hospital, Tangerang. The implementation of TDM by pharmacists based on the standards was 73%. The number of patients who met the inclusion criteria were 50 patients. Based on the diagnosis of the disease, patients with the potential of receiving TDM were patients with hypertension (10 patients; 20%), congestive heart failure (9 patients; 18%), and diabetes mellitus (8 patients; 16%). Whereas patients with both potential drug interaction and prescribed with more than 10 drugs was 29 patients (58%), patients with both potential drug interaction and diagnosed with three types of diseases was 19 patients (38%). The implementation of TDM in accordance with pharmaceutical service standards requires the competence of clinical pharmacist pharmacists with 2 years work experience. Potential drug interactions can be controlled by prioritizing TDM based on the number of drugs, complications of the disease and type of disease.
Isolasi dan Identifikasi Snyawa Geraniol dari Minyak Atsiri Tanaman Sereh Wangi Cymbopogon nardus (L.) Rendle Yelfi Anwar; Shirly Kumala; Elvina Dhiaul Iftitah; Partomuan Simanjuntak
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.403 KB) | DOI: 10.35814/jifi.v17i2.746

Abstract

Background: Indonesia is a major producer of several essential oils such as citronella oil, clove oil, cananga oil, vetiver oil, sandalwood oil, and patchouli oil. The Cymbopogon (Poaceae) genus has the most important components of essential oils, namely citral, geraniol, citronellol, citronellal. Geraniol is one of the most important chemical compounds in the aroma and fragrance industry and shows the nature of insecticides and natural insect repellents which show low toxicity and is suggested to represent a new class of chemoprevention agents for cancer, antimicrobial, anti-oxidant, anti-inflammatory and some vascular effects Transdermal drugs also attract the attention of researchers and scientists in the field of formulation. Objective: This study aimed to obtain geraniol compounds obtained from Cymbopogon nardus (L.) Rendle and identification. Methodology: The citronella, essential oil was obtained from the Cymbopogon nardus (L) Rendle plant using a water-steam distillation method, then fractionation was carried out and continued by purification isolation by column chromatography. Results: Based on the interpretation of IR data, Core magnetic resonance (proton and carbon RMI) and mass spectra for isolate 5 were determined as geraniol compounds. Conclusion: Geraniol can be obtained from Cymbopogon nardus (L) Rendle plants by water-steam distillation, fractionation and isolation methods.
Analisa Biaya Pengobatan Demam Tifoid Berdasarkan Clinical Pathway Di Rumah Sakit Harapan Bunda Pieter Hazmen; Shirly Kumala; Prih Sarnianto
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 13, No 2 (2019): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (65.646 KB) | DOI: 10.33533/jpm.v13i2.1314

Abstract

Clinical pathways are used in quality and cost control with indicators of length of stay. Cases of typhoid fever at Harapan Bunda Hospital include the criteria for the first rank of High Volume in 2018 and Problem Prone in the cost of treatment. The purpose of the study is to analyze the cost of treatment based on the implementation of clinical pathways in antibiotic therapy. Observational research with comparative studies of the use of injection antibiotics based on clinical pathway implementation. Retrospective data collection with dependent outcome therapy variables (cost and length of stay) while the independent variable regimen of antibiotic use. The population of typhoid fever patients according to the criteria for the implementation of clinical pathway ICD code (A0.10) 571 patients. Samples using total sampling that fulfills the criteria of direct inclusion are made into samples divided by the antibiotic regimen of the payment system, the group with the least amount excluded. There were 4 observation groups: generic ceftriaxone (n = 52) branded ceftriaxone (n = 51), generic cefotaxime (n = 53) and branded cefotaxime (n = 57) totaling 213 patients. Satatistic test results of length of stay (p> 0.05) explained that they did not have a significant difference, the average value (5.1596). Medical expenses for generic cefotaxime (Rp 4,072,002,6792), generic cherryax (Rp 4,479,480,4808), branded cefotaxime (Rp 6,945,258,3333) and branded ceftriaxone (Rp 7,296,933,5686). Conclusions based on JKN (AMiB) antibiotic payment systems are cheaper than general and the cheapest cefotaxime cheapest generic antibiotics.
Analisis Efektivitas Pemberian Konseling Dan Leaflet Terhadap Tingkat Kepatuhan Minum Obat Dalam Mengontrol Tekanan Darah Pada Pasien Hipertensi Di RSUD Dr. Adjidarmo Kabupaten Lebak Umilawati Rawi; Shirly Kumala; Wahyudi Uun
Jurnal Farmagazine Vol 6, No 1 (2019): Jurnal Farmagazine
Publisher : STF Muhammadiyah Tangerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47653/farm.v6i1.127

Abstract

Ketidak patuhan terapi hipertensi dapat menimbulkan komplikasi kronis. Konseling dan leaflet adalah bentuk edukasi yang dapat diberikan pada pasien hipertensi. Penelitian ini bertujuan untuk mengevaluasi efektivitas pemberian konseling dan leaflet terhadap tingkat kepatuhan melalui penurunan nilai tekanan darah sistole dan diastole dan penurunan skor kuesioner Morisky Medication Adherence Scale (MMAS-8) pada pasien hipertensi. Penelitian merupakan pre-experimental yang dilaksanakan secara prospektif mulai dari bulan April sampai Juni 2014 dan dilaksanakan di RSUD dr. Adjidarmo. Sampel penelitian adalah pasien hipertensi berjumlah 30 orang yang masuk kriteria inklusi dan eksklusi dan diberikan intervensi konseling dan leaflet. Kepatuhan diukur terhadap skor MMAS-8 dan nilai tekanan darah sistole dan diastolesebelum dan sesudah 30 hari pemberian intervensi. Hasil pengukuran dianalisis dengan menggunakan uji Wilcoxon untuk MMAS-8 dan Nilai tekanan darah sistole dan diastole. Hasil analisis menunjukkan ada perbedaan yang signifikan (p
Co-Authors . Desi . Suhaimi . Syamsudin . Yuliani Abdul Aziz Setiawan Adini, Syilvi Ahmad Subhan Aji, Nur Anwar Wahyudi Anwar, Yelfi Ariyani Kiranasari Ariyani Kiranasari Armansyah, Septiyana Lia Atut Ruswita Aulena, Desi Nadya Ayuditiawati, Meita Bekti, Novi Dyah Purnama Bekti, Novi Dyah Purnama Datubara, Martaulina Elisabet Delina Hasan Deni Rahmat Dian Indriani Dian Ratih L. Didik Tulus Dimas Adrianto Dosi Ahmad Yani Dwi Aryanti, Anindita Dwi Fitriyani, Dwi Dwi Hapsari Juniarti Dwi Windi Sapitri Elvina Dhiaul Iftitah Erlita Agustina Esti Mumpuni Esti Mumpuni, Esti Fachry Abda El Rahman Farida, Yunahara FARIDAH Febbyasi Megawaty Gabriel Gabriel Gabriel, Gabriel Gandes Winarni Gumilar Adhi Nugroho Halim, Marta Hasan, Delina Herru Setiawan Hersunaryati, Yetti Heru Santosa Heru Santosa Hery Prambudi Hesty Utami Hesty Utami R Huda, Badrul I Ketut Suada Iha Haryani Hatta Indarwati Indarwati Kurota Aini Lea P. Retnoningsih Leonardus B.S. Kardono Lestari Rahayu Mardiastuti, . Mardiastuti, . Marlin M. Raunsai Mochtar, Dede Mochtar, Dede Nancy Dewi Yuliana Nancy Raisa Nancy Raisa, Nancy Noor, Laili Savitri Nur Aji Nurmaini Nurmaini Partomuan Simanjuntak Partomuan Simanjuntak Pasanema, Dimas A. M. Pasanema, Dimas A. M. Pasaribu, Rita Kristina Pieter Hazmen Pratama, Anton Pratiwi Pujilestari Sudarmono Prih Sarnianto Priyo Wahyudi R, Hesty Utami Rachmani, Rum Rachmani, Rum RAHMAT, DENI Raihan, Dany Ramadaniati, Hesty Utami Restu Restiani Reubun, Yonathan Tri Atmodjo Reza Agung Sriwijaya Risma Marisi Tambunan, Risma Marisi Rita Kristina Pasaribu Robert Utji Rochimah Ros Sumarny RR. Ella Evrita Hestiandari Ruskar, Dandung Sarnianto, Prih Saru Noliqo Rangkuti Sembiring, Rinawati Sesilia A. Keban Sesilia Andriani Keban Setiadi, Feri Setiyarini, Setiyarini Shanny, Fransisca Shanny, Fransisca Simanjuntak, Partomuan Siswa Setyahadi Siswa Setyahadi Siti Jabal Mastura Sofyan Ramani Sri Widyastuti Stiani, Sofi Nurmay Sugiastuti, Fenty Suhaimi Suhaimi Suhaimi, . Syarifah Aini Teti Indrawati Umilawati Rawi Utami R, Hesty Violeta, Violeta Wahyudi Uun Widiyastuti, Asih Yati Sumiyati, Yati Yusransyah, Yusransyah Zaidan, Sarah