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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia Indonesian Journal of Cancer Chemoprevention agriTECH Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Majalah Obat Tradisional PROSIDING SEMINAR KIMIA INDONESIAN JOURNAL OF PHARMACY Jurnal Penelitian Sains Jurnal Fitofarmaka Indonesia Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Syntax Literate: Jurnal Ilmiah Indonesia Journal of Economic, Bussines and Accounting (COSTING) PENDIPA Journal of Science Education JRB-Jurnal Riset Bisnis PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JFIOnline Jurnal Ilmu Kefarmasian Indonesia Jurnal Kesehatan Poltekita : Jurnal Ilmu Kesehatan Jurnal Pendidikan dan Konseling Contagion: Scientific Periodical Journal of Public Health and Coastal Health Syntax Idea Jurnal Ners Jurnal Riset Kefarmasian Indonesia Enrichment : Journal of Management Lumbung Farmasi : Jurnal Ilmu Kefarmasian Sainstech Farma: Jurnal Ilmu Kefarmasian Jurnal Farmagazine Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia Daengku: Journal of Humanities and Social Sciences Innovation Jurnal Info Sains : Informatika dan Sains Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Medical Sains : Jurnal Ilmiah Kefarmasian Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Sciences of Pharmacy Jurnal Sosial dan Sains Sanskara Ekonomi dan Kewirausahaan West Science Business and Management Jurnal Farmasi Indonesia Parapemikir Jurnal Ilmiah Farmasi Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Sains dan Kesehatan Journal of Natural Product for Degenerative Diseases
Shirly Kumala
Univeristas Pancasila
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Phytochemical Screening and Toxicological Evaluation Using Brine Shrimp Lethality Test (BSLT) of Some Fractions of Prasman Leaves (Eupatorium triplinerve V) Extract Shirly Kumala; Dwi Windi Sapitri
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss1pp193-197

Abstract

Prasman leaves (Eupatororium triplinerve V) was well documented to have anti cancer benefit in Indonesian traditional medicine history. However, there were no scientific studies including toxicological assessment on the plan extract. The purpose of this study was to investigate the toxicological effect of some fractions of Prasman leaves methanol extract. Phytochemical screening by the Farnsworth method on powder and some fraction of the methanol extract were conducted followed by toxicity test using the “Brine Shrimp Lethality” test (BSLT) method. In the current study results, the phytochemical screening showed the presence of flavonoid, saponin, coumarin, tannin, steroid and volatile oil. LC50 of the n-hexane fraction 238.66 µg/mL, ethyl acetate fraction 24.42 µg/mL,  and n-butanol 64.10 µg/mL.Keywords : BSLT, Toxicity test, Eupatororium triplinerve
Isolasi dan Uji Antimikroba Substansi Bioaktif Mikroba Endofit Ranting Tanaman Johar (Cassia siamea Lamk.) Shirly Kumala; Syarmalina Syarmalina; Atut Ruswita Handayani
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 1 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Assay for secondary metabolite of endophytic fungi from Cassia siamea Lamk.. (Johar Plant) has been investigated. Endophyitic fungi are microorganisms that live asymtomatically within the living tissue of host plants. Endophytic fungi associate with plant, they can help the metabolism of host plant and produce potensial secondary metabolite. The purpose of this experiment were to investigate endophytic microbial agents. Isolate of endophytic fungi from twig of Cassia siamea Lamk., using direct seed method was carried out. The microbial activity was carried out with dilution method using paper disk and glass cylinder. The results showed 3 isolates of bacteria and 7 isolates of endophtyic fungi. The metabolite from three isolates of bacteria had the anti-microbial activity more poten toward Gram positive than Gram negative and four isolates of fungi had anti-Aspergillus agent.
Kapang Endofitik Tanaman Brucea javanica (L.) Merr yang Berpotensi menghasilkan Enzim Xilanase Wibowo Mangunwardoyo; Shirly Kumala; I. G.A. Ayu Wiratih
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 1 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Xylanase enzyme activity of endophytic fungi from Brucea javanica (L.) Merr has been done. Fruit, leave and stem of the plant collected from Bogor, Cianjur, and Tawangmangu which used in this study. To evaluate the enzyme activity the endophytic fungi isolate were inoculated on PDY medium and fermented at room temperature, shake at 150 rpm for 14 days. The supernatan were used to examine the extracellular enzyme and the biomass for intracellular enzyme. The DNS methods was used to test the enzyme activity. The results showed isolate 1.1.8; 1.2.17; 1.3.16 and 1.3.6 produced intra and extracellular xylanase enzyme. Isolate 1.1.6 produced only extracellular enzyme while isolate 1.2.2 did not produce both intra and extracellular enzyme.
Fermentasi Diam dan Goyang Isolat Kapang Endofit dari Brucea javanica L. Merr. dan Uji Aktivitas Antimikroba Shirly Kumala; Wibowo Mangunwardoyo; Peny Budiarti
JURNAL ILMU KEFARMASIAN INDONESIA Vol 3 No 2 (2005): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Exploring an antibiotic from microorganism is potential to discover the new antibiotic. The endophytic microorganism is one of the minimanufacturer producing the secondary metabolite such as antibiotic. Screening on the endophytic molds isolated from Brucea javanica L. Merr has been carried out. Fermentation in still and shake culture were used to produce the antibiotic using PDY medium. Antimicrobial activity was assayed using diffusion agar method. The results revealed that shake culture gave a better antimicrobial activity than still culture. Amount of eight isolate studied showed microbial activity against Gram positive bacteria: Staphylococcus aureus and Bacillus subtilis also Gram Negative bacteria: Escherichia coli and Salmonella typhi, however no microbial activity recorded againts Candida albicans. Isolate number 1.2 gave the highest antimicrobial activity on still culture and isolate number 1.7 gave the highest antimicrobial activity on shake culture. Further research should be carried out on identification and purification of the antimicrobial agent.
Evaluasi Pelayanan Pemantauan Terapi Obat di Rumah Sakit X Tangerang Febbyasi Megawaty; Shirly Kumala; Sesilia Andriani Keban
JURNAL ILMU KEFARMASIAN INDONESIA Vol 18 No 1 (2020): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.474 KB) | DOI: 10.35814/jifi.v18i1.783

Abstract

Therapeutic Drug Monitoring (TDM) is a clinical practice to increase the effectiveness of therapy and minimize the risk of adverse drug reactions (ADR). The purpose of this study is to describe the implementation of TDM, and whether it is in accordance with the Regulation of the Minister of Health of the Republic of Indonesia (Permenkes) Number 72 of 2016 concerning pharmaceutical service standards at hospitals and the evaluation of TDM services conducted by pharmacists on inpatients. This research covers a descriptive study with a collection of retrospective data from TDM documents of inpatients during the period of January–May 2016 at X Hospital, Tangerang. The implementation of TDM by pharmacists based on the standards was 73%. The number of patients who met the inclusion criteria were 50 patients. Based on the diagnosis of the disease, patients with the potential of receiving TDM were patients with hypertension (10 patients; 20%), congestive heart failure (9 patients; 18%), and diabetes mellitus (8 patients; 16%). Whereas patients with both potential drug interaction and prescribed with more than 10 drugs was 29 patients (58%), patients with both potential drug interaction and diagnosed with three types of diseases was 19 patients (38%). The implementation of TDM in accordance with pharmaceutical service standards requires the competence of clinical pharmacist pharmacists with 2 years work experience. Potential drug interactions can be controlled by prioritizing TDM based on the number of drugs, complications of the disease and type of disease.
Uji Aktivitas Enzim Xilanase Ekstraselular dan Intraselular Bakteri Endofitik Tanaman Brucea javanica (L.) Merr. Shirly Kumala; Wibowo Mangunwardoyo; Diah Dethriani
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 2 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Assay for xylanase enzyme activity of endophytic bacteria from Brucea javanica (L.) Merr. has been carried out. Parts of the plants used in this study were stem, leave and fruit. Samples were collected from Bogor, Cianjur and Tawangmangu. To evaluate the enzyme activity the fungi were shakefermented for 14 days. The supernatant were used to examine the extracellular enzyme and biomass for intracellular enzyme, using DNS methode to test the activity of enzyme. The results showed that from ten isolates only four isolates produced xylanase enzyme. Isolate 2.3.1 and 2.3.6 produced extra and intracellular enzyme, while isolate 2.1.15 and 2.3.13 only produced extracellular xylanase enzyme.
Penapisan Kapang Endofit Ranting Kayu Meranti Merah (Shorea balangeran Korth.) sebagai Penghasil Enzim Xilanase SHIRLY KUMALA; NUR ANNISA FITRI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 6 No 1 (2008): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Endophytic microbes are microorganisms that live in the plant. These microbes, are able to produce secondary metabolites, such as oligosaccharide degrading enzymes, growth factors, antibacterial substances and Xylanaze enzyme, an enzyme that can reduce the use of chlorine as bleaching agent in paper industries. This study Was aimed to isolate endophytic fungi that can produce extracellular xylanaze. Isolation of endophytic fungi from red memnti (Shorea balangeran Korth.) branches was carried out using surface sterilization and direct seeding methods on CMM (Corn Meal Malt) medium. Xylanaze was produced by using shaking fermentation methods on PDY (Potato Dextrose Yeast) for 12 days. Enzyme activity was assessed by using DNS (Dinitro salicylic acid) method. The results showed that of nine endophytic fungi isolates, eight have xylanaze enzyme activity, ranging from 1.279 µ/ml (highest) to 0.12 µ /ml (lowest).
Efek Imunostimulan Ekstrak Etanolik Herba Pegagan (Centella asiatica (L.) Urban) terhadap IgG Mencit Jantan Shirly Kumala; Aulia Tisna Dewi; Yun Astuti Nugroho
JURNAL ILMU KEFARMASIAN INDONESIA Vol 2 No 2 (2004): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

The immunostimulating effect of an ethanolic extract of Pegagan herbs to male mice has been determined. Thirty male mice divided into five groups (P1-P5) were induced by 1% sheep red cells for 14 days. P1, P2 and P3 were treated with three doses of an ethanolic extract 4.124 mg/20g BW, 8.25 mg/20g BW and 16.5mg/20gBW respectively. P4 was a positive control using Stimuno 0.55 mg/20g BW, and P5 was a negative control using distilled water Blood samples were taken at day 1, 8 and 15, and the concentration of IgG was measured by Radial Immunodiffusion method. A two way Anova showed no significant difference between P1, P2, and P5, but P3 was higher than P4. The obtained results showed that the ethanolic extract of 16,5 mg has a potent immunostimulating effect.
Produk Esktraseluler Isolat Kapang Endofi t C.1.1 dan C.3.3 dari Ranting Cempaka Kuning (Michelia champaca L.) Sebagai Antimikroba Adefira Elisyia Juniharti; Desi Nadya Aulena; Novi Yantih; Nur Miftahurrohmah; Shirly Kumala
JURNAL ILMU KEFARMASIAN INDONESIA Vol 19 No 1 (2021): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v19i1.985

Abstract

Michelia champaca L. is known to have effi cacy as an antimicrobial, antioxidant, and anticancer.In general, endophytic fungi that live in plant tissues is capable of producing secondary metabolites as theirhost plant. The objective of this research is to examine the antimicrobial potential of endophytic mold isolates C.1.1 and C.3.3 from yellow cempaka branches (Michelia champaca L.). Isolates obtained from previous research, fi rst was rejuvenated on PDA. Fermentation was carried out on each isolate for 12 days. The fermented supernatant was then extracted partially with n-hexane, ethyl acetate, and n-butanol solvent. The extract was then concentrated and tested for antimicrobial activity using disk diff usion and microdilution methods. N-butanol extract of endophytic fungi isolates of yellow champaca branches was able to inhibit the microbial growth with the largest inhibition area in isolate C.1.1 in Staphylococcus aureus ATCC 6538 (12.26 mm), Escherichia coli ATCC 8739 (11.40 mm), Candida albicans ATCC 10231 (10.50 mm), and Metichillin-Resistant Staphylococcus aureus (11.30 mm) with Minimum Inhibitory Concentration and Minimum Concentration of 12,5% . It was concluded that both endophytic fungi isolates of yellow champaca branches had the potential to inhibit the growth of pathogenic microbes.
Sinergisme Fraksi Butanol Metabolit Sekunder Kapang Endofit 1.3.11 dengan Doxorubicin dalam Modulasi Daur Sel T47D dan MCF-7 SHIRLY KUMALA; EDY MEIYANTO; MUTHI IKAWATI; RIRIS ISTIGHFARI JENIE
JURNAL ILMU KEFARMASIAN INDONESIA Vol 8 No 1 (2010): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

The synergic effects of n-butanolic fraction of secondary metabolite of endophytic fungus 1.3.11 (FB) and doxorubicin (Dox) on cell cycle regulation and the expression of Bcl-2 gene expression were investigated on MCF-7 and T47-D cells by iiow cytometry and immunocytochemical techniques respectively. The results showed that after 12 hours of incubation period with FB at its IC50 dose, MCF-7 cell cycle was inhibited at G1 phase while Dox inhibited the cell cycle at G2/M phase. Similar results were observed in T47-D cells when incubated with Dox and FB individually under the same treatment condition. Further treatment was then performed to these cells where both Dox and FB were combined at their IC50 and lC50 dose and added to incubate with the cells over 12 hours period. Interestingly, the modified treatment combination showed that MCF-7 and T47-D cell cycle regulation were inhibited at G2/M phase. Our immunocytochemical study also showed no significant inhibition suppression of Bel-2 gene expression in both MCF-7 and T47-D cells when compared with their corresponding positive control after treatment with FB and Dox or both combined FB and Dox at 1C50 and IC50 dosage over 15 hours incubation.
Co-Authors . Desi . Suhaimi . Syamsudin . Yuliani Abdul Aziz Setiawan Adefira Elisyia Juniharti Adini, Syilvi Afrini Saraswati Agan Syahrial Agung Dewantoro Ahmad Subhan Aji, Nur AMEILIA AMEILIA Anarisa Budiati Andri Prasetiyo Andria Agusta Andrianopsyah Mas Jaya Putra Anggelina Aprilia Pangalila Anggi Isnaasar Anny Victor Purba Anwar Wahyudi Anwar Wahyudi Anwar, Yelfi Ariyani Kiranasari Ariyani Kiranasari Armansyah, Septiyana Lia Asnah Tarigan Atut Ruswita Atut Ruswita Handayani Aulena, Desi Nadya Aulia Tisna Dewi Ayuditiawati, Meita Bekti, Novi Dyah Purnama Bekti, Novi Dyah Purnama Carrista Carrista Dandung Ruskar Datubara, Martaulina Elisabet Delina Hasan Deni Rahmat Desi Nadya Aulena Devana Devana Dewi Laxmi Diah Dethriani Dian Indriani Dian Ratih L. Dian Ratih Laksmitawati Didik Tulus Didik Tulus Dimas Adrianto Dimas Adrianto Dosi Ahmad Yani Dwi Aryanti, Anindita Dwi Fitriyani, Dwi Dwi Hapsari Juniarti Dwi Windi Sapitri Dwi Windi Sapitri EDY MEIYANTO Elvina Dhiaul Iftitah Elvina Dhiaul Iftitah ENDRO BUDI SISWANTO Erlita Agustina Erlita Erlita Esti Mumpuni Esti Mumpuni Esti, Esti Fachry Abda El Rahman Farida, Yunahara Faridah Faridah Fauzi Setyo Fandheo Fauzia Noprima Okta Febbyasi Megawaty Gabriel Gabriel Gabriel, Gabriel Gandes Winarni Gumilar Adhi Nugroho Halim, Marta Hasan, Delina Herru Setiawan Hersunaryati, Yetti Heru Santosa Heru Santosa Hery Prambudi Hesty Utami Hesty Utami Hesty Utami R Huda, Badrul I Ketut Suada I. G.A. Ayu Wiratih Iha Haryani Hatta Iha Haryani Hatta Indah Masri Indarwati Indarwati Khairunnisa Tamimi Kurota Aini Laili Savitri Noor Lea P. Retnoningsih Leonardus B.S. Kardono Lestari Rahayu Lies Putriana Lilik Sulastri Mardiastuti, . Mardiastuti, . Marlin M. Raunsai Meiyanto, Edy Meva Sari Chandra Miftahurrohmah, Nur Mochtar, Dede Mochtar, Dede Mulatsari, Esti Muthi Ikawati Nancy Dewi Yuliana Nancy Dewi Yuliana Nancy Raisa Nancy Raisa, Nancy Noor, Laili Savitri Noviyantih, Noviyantih Nur Aji NUR ANNISA FITRI Nurmaini Nurmaini Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Pasanema, Dimas A. M. Pasanema, Dimas A. M. Pasaribu, Rita Kristina Peny Budiarti Pieter Hazmen Pratama, Anton Pratiwi Pujilestari Sudarmono Prih Sarnianto Prih Sarnianto Priyo Wahyudi Qodriah, Rahmatul R, Hesty Utami Rachmani, Rum Rachmani, Rum RAHMAT, DENI Raihan, Dany Ramadaniati, Hesty Utami Raymond R. Tjandrawinata Reise Manninda Restu Restiani Reubun, Yonathan Tri Atmodjo Reza Agung Sriwijaya RIRIS ISTIGHFARI JENIE Risma Marisi Tambunan, Risma Marisi Rita Kristina Pasaribu Robert Utji Rochimah Ros Sumarny RR. Ella Evrita Hestiandari Ruskar, Dandung S.Farm., M.Farm., Apt, Sondang Khairani Sarah Zaidan Sarnianto, Prih Saru Noliqo Rangkuti Sembiring, Rinawati Septisetyani, Endah Puji Sesilia A. Keban Sesilia Andriani Keban Sesilia Andriani Keban Setiadi, Feri Setiawan, Abdul Aziz Setiyarini, Setiyarini Shanny, Fransisca Shanny, Fransisca Simanjuntak, Partomuan Siswa Setyahadi Siswa Setyahadi Siswa Setyahadi Siswa Setyahadi Siswa Setyahadi Siswa Setyahadi Siti Jabal Mastura Sofyan Ramani Sri Widyastuti Stiani, Sofi Nurmay Sugiastuti, Fenty Suhaimi Suhaimi Suhaimi, . Syamsudin Syamsudin Syarifah Aini Syarmalina Syarmalina Teti Indrawati Teti Indrawati Umilawati Rawi Utami R, Hesty Violeta, Violeta Vyacheslav Dushenkov Wahyudi Uun Wibowo Mangunwardoyo Wida Feriningsih Widiyastuti, Asih Yati Sumiyati, Yati Yetti Hersunaryati Yonathan Atmodjo Reubun Yun Astuti Nugroho Yuniarto, Ari Yusransyah, Yusransyah