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UJI EFEKTIVITAS EKSTRAK METANOL 80% BLACK GARLIC TUNGGAL SEBAGAI ANTIHIPERURISEMIA TERHADAP TIKUS: EFFECTIVENESS TEST OF 80% METHANOL EXTRACT SINGLE BULB BLACK GARLIC AS AN ANTIHYPERURICEMIC IN RATS Abdul Aziz Setiawan; Shirly Kumala; Dian Ratih L.; Nancy Dewi Yuliana; Rochimah; Saru Noliqo Rangkuti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 2 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i2.755

Abstract

Black garlic tunggal (BG) adalah produk fermentasi bawang putih tunggal pada suhu, dan kelembaban terkontrol selama 29 hari, sehingga mengalami reaksi Maillard. Penelitian terkait efektivitas antihiperurisemia BG belum pernah dilakukan. Penelitian ini bertujuan untuk mengetahui efektivitas antihiperurisemia dari ekstrak metanol 80% BG. Metodologi penelitian bersifat eksperimental berupa studi in vivo, hewan uji yang digunakan yaitu tikus putih jantan galur Sprague Dawley, dibagi menjadi 6 kelompok, kontrol normal, kontrol negatif, kontrol positif (Allopurinol 27 mg/kg BB tikus), kelompok perlakuan ekstrak metanol 80% BG dosis 62,5 mg/kg BB, 125 mg/kg BB, dan 187,5 mg/kg BB tikus. Sampel uji dan kontrol diberikan secara peroral selama 7 hari berturut-turut, lalu hari ke-8 diinduksi kalium oksonat secara intraperitonial dan pengambilan darah melalui sinus orbital, kemudian dilakukan pengujian dan dianalisis dengan oneway ANOVA. Hasil penelitian menunjukkan kelompok kontrol positif dan perlakuan I, II, III ekstrak metanol 80% black garlic tunggal mempunyai rata-rata sebesar 2,63; 3,99; 3,60 dan 2,75 mg/dl. Hasil Post Hoc LSD menunjukkan kelompok kontrol positif tidak memiliki perbedaan bermakna dengan kontrol normal dan ekstrak metanol 80% BG dosis 187,5 mg/kg BB tikus. Kesimpulan penelitian ini adalah studi in vivo ekstrak metanol 80% BG hari ke-29 mempunyai efektivitas sebagai antihiperurisemia pada tikus galur Sprague dawley. Kata Kunci: Black garlic tunggal, persen kadar asam urat, antihiperurisemia
Hubungan Rasionalitas Penggunaan Antibiotik Terhadap Outcome Klinik Pasien Pneumonia Komunitas Rawat Inap di RSUD. Dr. H. Abdul Moeloek Provinsi Lampung Tahun 2019 Widiyastuti, Asih; Kumala, Shirly; Utami R, Hesty; Pratama, Anton
Jurnal Kesehatan Vol 14 No 1 (2023): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v14i1.3483

Abstract

Pneumonia is a lung parenchyma disease caused by bacteria, fungi, viruses, and parasites. Irrational use of antibiotics will lead to antibiotic resistance, so the goal of therapy has yet to be achieved. This study evaluates the rationality of using community antibiotics for the clinical outcome of community pneumonia patients in RSUD. Dr. H. Abdul Moeloek.This research is descriptive-analytic with a cross-sectional design and retrospective data retrieval from medical records in 2019. The data analysis was carried out descriptively, and the correlation analysis of the rationale of antibiotic use with clinical outcomes using the Spearman test. The study's results of 144 analyzed showed the characteristics of community pneumonia patients, 78 male, and 66 female patients. The evaluation of the use of antibiotics using the ATC/DDD method showed that Ceftriaxon DDD was 46.80 DDD/100 patient-days, Azithromycin (23.91 DDD/100 patient-days), Levofloxacin (22.70 DDD/100 patient-days). With the Gyssen method, patients used antibiotics correctly (category 0) by 72.91% and incorrectly (categories I – VI) by 27.09%. Statistical analysis showed a significant relationship (p-value=0.003) between the rational use of antibiotics and patient clinical outcomes. Further research is needed regarding the incidence of Drugs Related Problems on the clinical effects of community pneumonia patients.
Colocasia esculenta L. Extract Increase Growth and Motility of Rotenone Induced Zebrafish (Danio rerio) Larvae Stunting Model Furqon, Muhammad; Kumala, Shirly; Farida, Yunahara; Zaidan, Sarah; Sumiyati, Yati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10670

Abstract

This study aims to determine the effect of Colocasia esculenta L on the growth and motility of zebrafish (Danio rerio) larvae after induction of rotenone as a stunting model. The Zebrafish have divided 3 groups i.e Normal (embryonic medium without rotenone induction), Negative Control (embryonic medium with rotenone induction), and Treatment Groups (embryonic medium with rotenone induction and Colocasia esculenta extracts, dose 30-60-90 ppm). The stunting model is designed by induction of 12.5 ppb rotenone to Zebrafish larvae. The growth of the larvae was observed by using a microscope to measure the body and head length. Evaluation of motility was carried out by recording the movement of the fish for 1 minute, and the pace was analyzed by using Video Tracker. The evaluations were observed at 3, 6, 9 dpf (day post-fertilization). Colocasia Esculenta Extract (CEE) groups have higher body lengths compared to the Negative Control Group. The lengths were 125.4 µm for CEE30, 119.4 µm for CEE60, and 121.4 µm CEE90 while the length for the Negative Control Group was 118.3 µm at 9 dpf. However, there were no different values for the ratio of head length to body length of all groups. The ratio was 1:6 at 3 dpf, and 1:4 at 6 and 9 dpf. Moreover, the motility was higher in CEE groups compared to the Negative Control at 3, 6, 9 dpf. The body length and motility of Zebrafish larvae higher compare to the Negative Control Group showing the potential of Colocasia esculenta extract on growth and enhancement of motoric activity.
Evaluation of Total Flavonoid Content, Xanthine Oxidase Inhibition, and Uric Acid Inhibitory Activity In Vitro and In Vivo of Ethanol Extract of Sida rhombifolia Aulena, Desi Nadya; Kumala, Shirly; Abdillah, Syamsudin; Rahmat, Deni; Sugiastuti, Fenty; Indarwati, Indarwati; Fitriyani, Dwi
Sciences of Pharmacy Volume 3 Issue 3
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0303255

Abstract

Secondary metabolites content in Sida rhombifolia L. (SR) are believed to inhibit the xanthine oxidase (XO) enzyme, which is responsible for converting hypoxanthine and xanthine into uric acid. This study aimed to compare flavonoid levels, XO inhibitory activity, and the reduction of uric acid levels of sidaguri herb, stem, leaves, and root extract both in vitro and in vivo. The research results showed that the highest total flavonoid content in SR was found in the leaves, at 21.29±0.08 mg/QE/g extract. The IC50 values were as follows: roots (EESRR 1096.07±1.07 ppm), stems (EESRS 561.62±7.01 ppm), leaves (EESRL 101.84±0.63 ppm), and herbs (EESRH 104.70±3.50 ppm). EESR can inhibit the XO enzyme and has potential as an anti-hyperuricemia agent. The best total flavonoid content and IC50 values were observed in EESRL, which are 21.29±0.08 mg/QE/g and 101.84±0.63 ppm, respectively. EESRL at a dose of 400 mg/kg BW has the equivalent ability to reduce blood uric acid levels in mice when compared to the positive control group. Based on these findings, it can be concluded that EESRL has significant potential as a natural XO inhibitor and can be considered a promising candidate for the development of anti-hyperuricemia treatments.
Evaluation of Drug Procurement Pattern and Drug Availability for JKN Patients at X Hospital and Y Hospital, Cirebon District, January-December 2022 Period Siti Jabal Mastura; Prih Sarnianto; Shirly Kumala; Dosi Ahmad Yani; Hery Prambudi
Daengku: Journal of Humanities and Social Sciences Innovation Vol. 4 No. 5 (2024)
Publisher : PT Mattawang Mediatama Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35877/454RI.daengku2569

Abstract

The era of National Health Insurance (JKN) brought changes in the health care system, which required hospital pharmacists to adapt to drug regulations and planning. Procurement planning and procedures have a significant impact on the availability of drugs and the financial situation of the hospital in drug management. One of the most crucial aspects of the hospital is the availability of guaranteed goods and sufficient drug supplies in order to provide the best service. This research was conducted to evaluate the procurement and availability of drugs at X Hospital as a public hospital and Y Hospital as a private hospital in Cirebon Regency. The analysis used in this study is descriptive-evaluative, namely the research method used to obtain data in a descriptive form and a systematic analysis is carried out to evaluate based on the descriptive data. Data collection was carried out by means of in-depth interviews with the hospital and distributors as external parties who also contributed to fulfilling the availability of drugs. The data is also complemented by the results of field observations and review of document reports related to the National Formulary (Fornas), Hospital Formulus (Forkit), and Drug Needs Plan (RKO). The results of this study found that the Drug Needs Plan, procurement and availability of drugs at X Hospital and Y Hospital were in accordance with the standards. This shows that most of the drugs available and given to patients are in accordance with the drugs listed in the National Formulary. There is a difference in the pattern of procurement. Hospitals conduct procurement twice a month, but private hospitals conduct procurement patterns twice a week. The two hospitals both use the SIMRS and Teramedik management information systems for the evaluation process of drug procurement and availability
Toxicity of Sida rhombifolia L. 96% ethanol extract based on LD50 and macropathological examination of mice's organs Aulena, Desi Nadya; Kumala, Shirly; Abdillah, Syamsudin; Rahmat, Deni; Zaidan, Sarah; Fitriyani, Dwi; Raihan, Dany
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 1 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i1.1463

Abstract

The Indonesian people empirically use Sidaguri (Sida rhombifolia L.) in medicine, such as anti-hyperuricemia. Herbal-based treatment is currently much preferred. Drug metabolism, in general, mainly occurs in the liver, so the possibility of damage to this organ is high. This study aims to determine the acute toxicity category of 96% ethanol extract of sidaguri based on the LD50 value, changes in body weight, changes in organ weight, and macro pathology in the organs of male DDY mice in vivo. The method used in this research was experimental with a fixed dose design following the Indonesian Food and Drug Authority regulations regarding guidelines for in vivo non-clinical toxicity tests. The test group was divided into six dose groups (standard, 50, 300, 2000, 5000, and 15000 mg/kg Body of weight). Observations were made for 24 hours. Observations were continued for 14 days on death parameters, toxicity symptoms, body weight, and relative organ weights using five main organs (heart, lungs, liver, spleen, and kidneys, and pathological examination). The acute toxicity test results showed no death in all dose treatment groups. Macropathological analysis did not show abnormalities in organs in all groups of mice. LD50 value is more than 15000 mg/kg. Sidaguri 96% ethanol extract is safe and Practically non-toxic.
Branded Drug Planning Based on (Combination) of Consumption Methods With ABC Analysis and Reorder Point (ROP) its Effect on Inventory Value and Turn Over Ratio (TOR) in the Pharmacy Installation of X Hospital, Bekasi Muhammad Sapuan; Shirly Kumala; Sri Widyastuti
Jurnal Info Sains : Informatika dan Sains Vol. 15 No. 01 (2025): Informatika dan Sains , 2025
Publisher : SEAN Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Planning drug needs in the Pharmacy Installation at Hospital X Bekasi, using the consumption method. This causes shortages and excess stock of branded drugs so that the drugs are not used and expire. The high value of branded medicine inventory at Hospital The aim of this research is to determine the difference in inventory value and Turn Over Ratio (TOR) of fast moving branded drugs after testing the application of a branded drug planning model based on a combination of consumption methods with ABC analysis and reorder points compared to the planning before testing in Hospital Pharmacy Installations. X Bekasi. The type of research carried out was pre-experimental using analytical descriptive methods. The approach used in this research is cross sectional. In-depth interviews and Focus Group Discussions were also conducted. The trial was carried out on fast moving ABC branded drugs for 3 months, namely June-August 2023. The quantitative approach was by calculating the inventory value and TOR value of fast moving group A, B and C branded drugs, before and after the trial. The research results prove that the application of model trials can reduce the inventory value of ABC fast moving branded drugs from IDR 124,848,282 to IDR 122,454,522, increase the TOR of ABC fast moving branded drugs from 5.66 to 8.12 and obtain an efficiency of IDR 2,393,760. The conclusion of this research is that planning for fast moving ABC branded drugs based on a combination of consumption methods with ABC and ROP analysis has been proven to reduce inventory value and increase TOR and obtain an efficiency of 1.92%.
Optimalisasi Lembaga Farmasi TNI AL Dalam Produksi Sediaan Farmasi Guna Mendukung Ketahanan Kesehatan Ruskar, Dandung; Kumala, Shirly; Noor, Laili Savitri
PendIPA Journal of Science Education Vol 9 No 1 (2025): February
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.9.1.132-139

Abstract

RIPIN (National Industrial Development Master Plan) 2015-2035 has placed the Pharmaceutical Industry as one of the priority industries to be developed. Meanwhile, the results of the ASEAN Summit in September 2023 held in Jakarta stated that Indonesia had the lowest score for indicators of drug availability and costs, which was 54.02. The existence of BPJS Kesehatan in 2014 greatly reduced the role and function of the Navy Pharmaceutical Institution as the pharmaceutical industry owned by the TNI AL. Currently, Lafial only produces pharmaceutical preparations for health support whose need is very limited because the state budget received is also reduced. In order to support and increase the availability of medicines in Indonesia, the role and function of Lafial should be utilized. The purpose of this study was to design the right business strategy for Lafial to meet the needs of national medicines while reducing dependence on the state budget through SWOT analysis and QSPM matrix. This was qualitative research using a descriptive analysis approach to further explore the internal and external parameters factors of Lafial. The data collection technique used was triangulation from in-depth interviews and FGDs on key personnel pharmacists as well as questionnaires through Google Forms for all pharmacists outside key personnel and secondary data collection. The results of the study on the strength component score was 2.022; the weakness component was 1.540; the opportunity component was 2.020 and the threat component was 1.673. With these results, Lafial's position is in cell number I of the IE matrix and quadrant I of the SWOT quadrant. The strategy analysis using QSPM provides that the strategy of sharpening market penetration through coordination with the Officials and Heads of the Navy health facilities to use Lafial's finished products has the highest TAS value with a value of 5,611. Then the strategy of developing the market through coordination with the Ministry of Health and the Ministry of Finance to be able to produce and deposit PNBP has a TAS value of 5.204. The last strategy is a strategy to increase operational efficiency through coordination with the state-owned and private pharmaceutical industry through drug toll manufacturing activities with a TAS value of 4.332.
RANCANGAN STRATEGI LEMBAGA FARMASI TNI AL DALAM MEMASUKI PASAR FARMASI NASIONAL: Rancangan Strategi Lembaga Farmasi TNI AL Dalam Memasuki Pasar Farmasi Nasional Ruskar, Dandung; Kumala, Shirly; Noor, Laili Savitri
PendIPA Journal of Science Education Vol 9 No 1 (2025): February
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.9.1.240-249

Abstract

The growth of the pharmaceutical market in Indonesia provides a positive trend. It is increasing along with the increase in population, public awareness of health, and people's purchasing power. Supported by the government's seriousness to improve services in the health sector, making the pharmaceutical industry one of the priority non-oil and gas industries and included in the National Industrial Development Master Plan (RIPIN) 2015-2035. This positive market growth is in stark contrast to the current growth of the pharmaceutical industry. According to data from the Central Statistics Agency (BPS), the pharmaceutical industry in Indonesia has experienced very slow growth, namely by 0.7% in 2022 and by 0.1% in 2023. The ASEAN Summit in September 2023 also placed Indonesia as a country with the lowest score for the availability of medicines. The Navy Pharmaceutical Institute (Lafial) as a pharmaceutical industry owned by the Navy has great potential to be utilized. Moreover, Lafial's current condition requires increasing production capacity to get other budgets apart from the state budget by participating in the Indonesian pharmaceutical market. This research aims to obtain a business strategy for Lafial to be able to participate in the Indonesian pharmaceutical market and despite the current less conducive conditions. This research uses SWOT analysis as a method which is then followed by using the QSPM matrix. This research is a qualitative descriptive analysis to explore the components of internal and external factors owned by Lafial. The data used are data obtained from interviews, FGDs, questionnaires, and secondary data. This study provides results on the strength component score value of 1.50; the weakness component is 1.98; the opportunity component is 2.08 and the threat component is 1.67. With these results, Lafial's position is in quadrant III of the SWOT quadrant. A strategy analysis using QSPM gave the result that the market switch strategy by improving other functions other than production gave the highest TAS value of 5.05. The second strategy is increasing production capacity through toll manufacturing activities with a TAS value of 4.89. The third strategy is building cooperation with the TNI Navy cooperative with a TAS value of 4.81 and the last strategy is to transform into a sub-task force that can deposit PNBP with a TAS value of 4.22.
In Silico and In Vitro Antibiofilm Activity of Zingiber montanum Ethyl Acetate Fraction Against Propionibacterium acnes: Antibiofilm of Zingiber montanum Aji, Nur; Kumala, Shirly; Mumpuni, Esti; Rahmat, Deni
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Propionibacterium acnes (P. acnes), a symbiotic bacterium involved in the development of acne, is recognized for its capacity to develop resistance due to its ability to generate biofilms. According to a previous investigation, the ethyl acetate fraction derived from Zingiber montanum exhibited anti-bacterial properties against P. acnes. Nevertheless, the causative role of biofilm formation in its resistance was not previously recognized. This study aims to ascertain the antibiofilm efficacy using in silico and in vivo methodologies. In this study, in silico examination utilized molecular docking to inhibit the quorum sensing mechanism by blocking the autoinducer 2 (AI-2) signal. The target protein was AI-2 binding periplasmic protein LuxP (PDB code: 4YRZ). Following that, an in vitro test was conducted using the crystal violet staining method, which was read using a microplate reader at a wavelength of 600 nm. The readings were expressed as percent inhibition and IC50. The in silico results showed that there were six compounds in the ethyl acetate fraction that have the potential to inhibit AI-2 signaling. Curcumin showed the highest inhibitory activity. Meanwhile, the in vitro results revealed that the ethyl acetate fraction of Z. montanum obtained the minimum biofilm inhibitory concentration (MBIC) of 2.5 % (w/v) and IC50 = 0.28 % (w/v). It can be concluded that the ethyl acetate fraction of Zingiber montanum could inhibit the biofilm formation of P. acnes.  
Co-Authors . Desi . Suhaimi . Syamsudin . Yuliani Abdul Aziz Setiawan Adini, Syilvi Ahmad Subhan Aji, Nur Anwar Wahyudi Anwar, Yelfi Ariyani Kiranasari Ariyani Kiranasari Armansyah, Septiyana Lia Atut Ruswita Aulena, Desi Nadya Ayuditiawati, Meita Bekti, Novi Dyah Purnama Bekti, Novi Dyah Purnama Datubara, Martaulina Elisabet Delina Hasan Deni Rahmat Dian Indriani Dian Ratih L. Didik Tulus Dimas Adrianto Dosi Ahmad Yani Dwi Aryanti, Anindita Dwi Fitriyani, Dwi Dwi Hapsari Juniarti Dwi Windi Sapitri Elvina Dhiaul Iftitah Erlita Agustina Esti Mumpuni Esti Mumpuni, Esti Fachry Abda El Rahman Farida, Yunahara Faridah Faridah Fauzi Setyo Fandheo Febbyasi Megawaty Gabriel Gabriel Gabriel, Gabriel Gandes Winarni Gumilar Adhi Nugroho Halim, Marta Hasan, Delina Herru Setiawan Hersunaryati, Yetti Heru Santosa Heru Santosa Hery Prambudi Hesty Utami Hesty Utami R Huda, Badrul I Ketut Suada Iha Haryani Hatta Indarwati Indarwati Kurota Aini Lea P. Retnoningsih Leonardus B.S. Kardono Lestari Rahayu Lies Putriana Mardiastuti, . Mardiastuti, . Marlin M. Raunsai Mochtar, Dede Mochtar, Dede Nancy Dewi Yuliana Nancy Raisa Nancy Raisa, Nancy Noor, Laili Savitri Nur Aji Nurmaini Nurmaini Partomuan Simanjuntak Partomuan Simanjuntak Pasanema, Dimas A. M. Pasanema, Dimas A. M. Pasaribu, Rita Kristina Pieter Hazmen Pratama, Anton Pratiwi Pujilestari Sudarmono Prih Sarnianto Priyo Wahyudi R, Hesty Utami Rachmani, Rum Rachmani, Rum RAHMAT, DENI Raihan, Dany Ramadaniati, Hesty Utami Restu Restiani Reubun, Yonathan Tri Atmodjo Reza Agung Sriwijaya Risma Marisi Tambunan, Risma Marisi Rita Kristina Pasaribu Robert Utji Rochimah Ros Sumarny RR. Ella Evrita Hestiandari Ruskar, Dandung Sarnianto, Prih Saru Noliqo Rangkuti Sembiring, Rinawati Sesilia A. Keban Sesilia Andriani Keban Setiadi, Feri Setiyarini, Setiyarini Shanny, Fransisca Shanny, Fransisca Simanjuntak, Partomuan Siswa Setyahadi Siswa Setyahadi Siti Jabal Mastura Sofyan Ramani Sri Widyastuti Stiani, Sofi Nurmay Sugiastuti, Fenty Suhaimi Suhaimi Suhaimi, . Syarifah Aini Teti Indrawati Umilawati Rawi Utami R, Hesty Violeta, Violeta Wahyudi Uun Widiyastuti, Asih Yati Sumiyati, Yati Yusransyah, Yusransyah Zaidan, Sarah