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All Journal HAYATI Journal of Biosciences Buletin Peternakan Jurnal Kesehatan Lingkungan indonesia Jurnal Ilmu dan Teknologi Hasil Ternak Biogenesis: Jurnal Ilmiah Biologi Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika AGRIVITA, Journal of Agricultural Science JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Widyariset Jurnal Kebidanan Dan Kesehatan Tradisional Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Farmasi Sains dan Komunitas Universa Medicina Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Bunda Edu-Midwifery Journal (BEMJ) AL-HAYAT: Journal of Biology and Applied Biology Jambura Edu Biosfer Journal Jurnal Medika Malahayati Jurnal Doppler BIO-EDU: Jurnal Pendidikan Biologi Widyariset REKASATWA : Jurnal Ilmiah Peternakan Jurnal Pengabdian Masyarakat Asia Jurnal Empathy Pengabdian Kepada Masyarakat Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) Indonesia Journal of Midwifery Sciences Livestock and Animal Research SENTRI: Jurnal Riset Ilmiah Genius Midwifery Journal Jurnal Pengabdian Masyarakat Jajama (JPMJ) JOURNAL OF Mother and Child Health Concerns Nurul Ilmi : Journal of Health Sciences and Midwifery Advances in Cancer Science Life Science and Biotechnology Jurnal Agripet Journal of Agriprecision & Social Impact Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Kuswati Kuswati
Departemen Produksi Ternak, Fakultas Peternakan Universitas Brawijaya, Malang.
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Journal : Universa Medicina

 1 
Black sugarcane decoction reduces rat brain ischemia Handayani, Ety Sari; Nugraha, Zainuri Sabta; Nurmasitoh, Titis; Kuswati, Kuswati; Ahsani, Dwi N.; Nanda, Ajeng G.
Universa Medicina Vol 35, No 1 (2016)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2016.v35.40-45

Abstract

BackgroundThere are people in Yogyakarta, who use black sugarcane decoction (BSD) to prevent stroke. BSD contains policosanol and antioxidants. It has been proven that policosanol can reduce global ischemia in Mongolian gerbils. This study aims to evaluate the effect of BSD on brain ischemia in a rat stroke model. MethodsA laboratory experiment using eighteen 3-month old male Wistar rats without any defects, of 175-250 g body weight. Brain ischemia was produced by a 20-minute bilateral carotid communis artery oclusion (BCCAO).  Using a rat stroke model, brain ischemia was produced by a 20-minute BCCAO. The rats were randomized into three groups: BSD treated stroke model rats (group 1), non treated stroke model rats (group 2), and sham operated rats (group 3). BSD was administered by gavage for 1 week before BCCAO. Decapitation of rats was performed two hours post BCCAO. Brain tissues were stained with 2,3,5-triphenyltetrazolium chloride (TTC). Ischemic areas were analyzed using Image J softwere. Statistical analysis was conducted by one way ANOVA test.ResultsThe mean percentages of rat brain ischemic area differed between group 3 (0.0 ± 0.0%), group 2 (3.13 ± 0.59%) and group 1 (1.15 ± 0.47%) p =0.001). Post hoc test showed that there was no difference between group 3 with group 1. Instead, there was a significant difference between  group 2 and the other groups.ConclusionThe administration of BSD reduced rat brain ischemia after bilateral carotid artery ligation.
Propolis inhibited Bax expression and increased neuronal count of hippocampal area CA1 in rats receiving sodium nitrite Kuswati, Kuswati; Handayani, Ety Sari; Nugraha, Zainuri Sabta; Rahmanti, Fishella Aprista; Wicaksana, Zulfikar Loka; Zhafirrahman, Muhammad
Universa Medicina Vol 38, No 2 (2019)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.491 KB) | DOI: 10.18051/UnivMed.2019.v38.73-80

Abstract

BackgroundSodium nitrite induces hypoxia and oxidative stress in the hippocampus, decreasing the number of neurons in the hippocampus and cognitive function. Propolis contains chrysin that has antioxidant effects that are expected to inhibit neuronal damage in the hippocampus. This study aims to determine the effects of propolis on the expression Bcl-2-associated X protein (Bax) and the number of neurons in the rat hippocampus receiving sodium nitrite.MethodsThis study of laboratory experimental design was conducted on 18 male Wistar strain rats (Rattus norvegicus), they were randomized into 3 groups: one control group (K) received sodium nitrite and two intervention groups  (P1 and P2) received sodium nitrite and propolis at doses of 100 and 200 mg/kgBW. Treatment with sodium nitrite and propolis were given for 60 days, followed by termination. The number of neurons and Bax expression in the hippocampal CA1 area were measured. One-way ANOVA was used to analyze the data.ResultsThere were significant differences in Bax expression between group K and groups P1 and P2 (p<0.001). The lowest number of neurons in the hippocampal CA1 area was in the K group. There were significant differences in the number of neurons between control (K) group and groups P1 and P2 (p<0.001).ConclusionPropolis inhibited the expression of Bax and decreased the number of neurons in the hippocampal CA1 area of rats receiving sodium nitrite. This study provides information about the benefits of propolis as an antioxidant in the brain.
Co-Authors A. P. A. Yekti Abdul Muhaimin Abdul Rohman ACHMAD FARAJALLAH Aditya Kurniawan Agus Susilo Agus Susilo Ahmad Furqon Ahmad Furqon Ajeng G. Nanda, Ajeng G. Ali Mahfud Ali mahfud Alifian Ibnu Ansori Andi Murlina Tasse Andra Widiastutik Andriawan Soni Hantoro Anggun Permata Sari Ardhina Firdausi Aris Sutomo Aryogi Aryogi Aryogi Aryogi Asri Nurul Huda Asri Nurul Huda Aulia Puspita A Yekti Aulia Puspita Anugra Yekti Awang Tri Satria B. Susilo Bayu Dewantoro Putro Soewandi Bella, Frimita Chamelia Putri, Chamelia D. Muhammad Dandy Prasetyo Daud, Nadhiratuzzahra Alifiah Dewi , Meinasari kurnia Dian Kumalasari, Elis Dicky Pamungkas Doni Herviyanto Dwi N. Ahsani, Dwi N. Dwi Retna Prihati, Dwi Retna Dwi Susan Setiyani Dwijayanti, Indah Dyah Perwitasari Eko Nugroho Elvira, Ajeng Erin Ayu Octaviani Erlangga Arfiyan Nur Erlia Narulita Ety Sari Handayani Fatimah, Jesy Febrianti, Riska Ayu Ferdy Saputra Fiarani, Hajar Syifa Gani, Tifany Sonia Gatot Ciptadi Hamidah Ikhwan, Salma Nur Hary Nugroho Henik Istikhomah, Henik Hermansyah, Alfandy Herviyanto, Doni Herviyanto, Dony Husnassabil, Ahmad Hadin Ikhsanuddin Ikhsanuddin Ikhsanuddin, Ikhsanuddin Imam Mudakir Indrayani, Tsaniya Ahda Indriyani Indriyani J. A. E. Noor Joko Waluyo Kaiin, Ekayanti Mulyawati Khairul Rasyad Khairunnisa, Icha Kurniaesa, Titah Ulya Kusmartono Kusmartono L. A. Sunarto Latifah Hanum Lina Winarti M. Luthfi M. Nasich Marini . Marjuki Marjuki Mashudi Mashudi Mashudi Mashudi Maulid, Shintia Sukmawati Cahya Moch Nasich Moch Sasmito Djati Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Mohammad Wildan Habibi, Mohammad Wildan Muhamad Iqbal Zajulie Muhammad Irvan Ali Muhammad Zainul Hanif Mukhlas Agung Hidayat N. Isnaini Nareswara Hapsari Ndaru Pranowo Widiasmoro Nisa’us Sholikah noviyani, ernita prima Nurgiartiningsih, V. M. A. Nurjazuli Nurjazuli Nurmasitoh, Titis Nurrasyidah, Rahmi Nurul Huda, Asri Nurul Insani Shullia Nurul Isnaini Nurul Layla Oktriyani, Erni Onni Meirezaldi Pamungkas, Atik Mahmudah Aji Paskah Partogi Agung Pratiwi, Herlina Priyo Sugeng Winarto Puguh Surjowardojo Pujiastuti Pujiastuti, Pujiastuti Purwanto, Didiek Putri Utami Putri Utami Putri, Aprillia Ika Putri, Rizqi Wulandari R Sa’diyah, Kusnul Rachmawati, Sela Rahmanti, Fishella Aprista Raihanny, Yasmin Ravenska Ravenska Rifai Mustofa Rini Dwi Wahyuni Rini Dwi Wahyuni Risky Amalia Rosary Rizki Prafitri Rizki Prafitri Rofidaa, Asta Rosalinda, Julitha D Rosalinda, Julitha Dwi Rusdiana, Rina Sa'diyah, Kusnul Rodhiyatul Safitri, Putri Fina A’rafiani Safitri, Sinda Mercy Saputra, Muhammad Rizky Sari, Agustina Sari, Prima Ratna Siti Hodijah Sri Wahjuningsih Sucik Maylinda Sucik Maylinda Sugesti, Madiyan Surjowadojo, Puguh Sutomo, Aris Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Syahruddin Said T. Susilawati T. Susilawati Tri Eko Susilorini Tri Eko Susilorini tri wahyuni Trinil Susilawati Triwik Sri Mulati Veronica Margareta Ani Nurgiartiningsih Wibowo, Abdilla Fi Qisthy Wicaksana, Zulfikar Loka Widi Nugroho Widiasmoro, Ndaru Pranowo Wike andre Septian Wiranto Wiranto Yadi Malda Yayuk Fatmawati, Yayuk Yulian, Ria Zahro, Aliza Zainuddin Zainuddin Zainuddin, Zainuddin Zainuri Sabta Nugraha, Zainuri Sabta Zhafirrahman, Muhammad Zulfaini Shamad