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ISOLASI PIGMEN KAROTENOID DARI KEPITING Grapsus sp. (CAROTENOID PIGMENTS ISOLATION FROM CRABS Grapsus sp.) Dias Diasasthisa; Darus J Paransa; Desy MH Mantiri; Antonius Rumengan; Veibe Warouw; Meiske Salaki
JURNAL PESISIR DAN LAUT TROPIS Vol. 7 No. 2 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.2.2019.24216

Abstract

Carotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.             Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK  yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid.  
ANALISIS LOGAM TIMBAL (Pb) PADA Kappaphycus alvarezii (Doty) ALGA MERAH YANG DI BUDIDAYA DI TELUK TOTOK MINAHASA TENGGARA, SULAWESI UTARA Filemon Hosea; Desy M H Mantiri; James J H Paulus; Rizald M Rompas; Frans Lumoindong; Joppy D Mudeng
JURNAL PESISIR DAN LAUT TROPIS Vol. 7 No. 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24430

Abstract

Macroalgae are chlorophyll plants, consisting of thallus and can live in polluted waters. The purpose of the study was to analyze the lead metal (Pb) in the water column and thallus red algae Kappaphycus alvarezii which was cultivated in a certain period of time in the waters of Totok Bay. The cultivation method used is the bottom method. The algae weight is measured every two weeks. Water quality parameters and lead metal analysis (PB) are carried out in the Baristan laboratory. The results obtained are algae can grow according to the time period. Water quality can affect algae growth. Lead metal content in water is detected high enough as well as in thallus algae in the 3rd week. Keywords : Kappaphycus alvarezii, Lead metal, Water quality, Totok Bay Makroalga merupakan tumbuhan yang berklorofil dan tubuhnya berupa thallus serta mampu hidup pada perairan tersemar. Penelitian ini dilakukan dengan tujuan untuk menganalisis kandungan logam timbal (Pb) dalam air dan thallus alga merah Kappaphycus alvarezii yang dibudidayakan dalam periode waktu tertentu di perairan Teluk Totok. Metode budidaya pada yang digunakan adalah metode lepas dasar (bottom method). Pengukuran berat alga dilakukan setiap dua minggu. Beberapa parameter kualitas air dan analisis logam timbal (Pb) dilakukan di Laboratorium Baristan. Hasil yang diperoleh yaitu alga dapat tumbuh sesuai dengan periode waktu. Kualitas air dapat mempengaruhi pertumbuhan alga.Kandungan logam timbale pada air terdeteksi cukup tinggi demikian juga pada thallus alga di minggu ke-3. Kata kunci : Kappaphycus alvarezii, Logam Timbal, Kualitas air, Teluk Totok 
ANALISIS PIGMEN KLOROFIL PADA ALGA Kappaphycus alvarezii YANG DIBUDIDAYAKAN DI TELUK TOTOK KABUPATEN MINAHASA TENGGARA Raja Doli; Desy MH Mantiri; Darus S J Paransa; Kurniati Kremer; Rosita Lintang; Sipriani Tumembow
JURNAL PESISIR DAN LAUT TROPIS Vol. 8 No. 1 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.1.2020.27399

Abstract

Macroalgae is a chlorophyll plant and its body is thallus and is able to live in polluted waters. The research was conducted with the intention of knowing the content of chlorophyll pigment and comparing the concentration of chlorophyll on Kappaphycus alvarezii using the formula Jeffrey and Humprey (1975) and Harbone (1987). The results obtained to separate the chlorophyll pigment on Kappaphycus alvarezii using the thin layer chromatography (KLT) method, thus obtained the pigment identified from Kappaphycus alvarezii namely chlorophyll a. Keywords: Kappaphycus alvarezii, thin-layer chromatography. Chlorophyll.AbstrakMakroalga merupakan tumbuhan yang berklorofil dan tubuhnya berupa thallus serta mampu hidup pada perairan tercemar. Penelitian ini dilakukan dengan tujuan untuk mengetahui kandungan pigmen klorofil dan membandingkan konsentrasi klorofil pada Kappaphycus alvarezii menggunakan rumus Jeffrey dan Humprey (1975) dan Harbone (1987). Hasil yang diperoleh untuk memisahkan pigmen klorofil pada Kappaphycus alvarezii menggunakan metode Kromatografi Lapis Tipis (KLT), sehingga diperoleh pigmen yang teridentifikasi dari Kappaphycus alvarezii yaitu klorofil a. Keywords : Kappaphycus alvarezii, Kromatografi Lapis Tipis. Klorofil.
PIGMEN KAROTENOID PADA KEPITING Ozius sp Sriyati Enjelina Sibarani; Darus S J Paransa; Kurniati Kemer; Desy M.H Mantiri; Natalie D.C Rumampuk; Sipriana Siana Tumembow
JURNAL PESISIR DAN LAUT TROPIS Vol. 8 No. 1 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.1.2020.27457

Abstract

Carotenoid pigments are natural dyes that have an influence on organism. For the human body, carotenoids function as antioxidants, anticancer, anti-bacteria and help maintain eye health. Carotenoid pigments are found in groups of bacteria, fungi, molds, algae and green plants and one of them in crustaceans is the crab Ozius sp. To determine the metabolic process of carotenoid pigments, each crab organ of Ozius sp. isolated using thin layer chromatography separation method to determine the type of pigment contained therein. The type of pigment identified in the crab Ozius sp. females with the TLC method namely: β-carotene, Cantanxhantine, and β-cryptosanthin.Keywords: Ozius sp, Thin Layer Chromatography, Carotenoids Pigments. Pigmen karotenoid adalah zat warna alami yang memiliki pengaruh terhadap organisme. Bagi tubuh manusia karotenoid berfungsi sebagai antioksidan, antikanker, anti bakteri dan membantu memelihara kesehatan mata. Pigmen karotenoid banyak ditemukan pada kelompok bakteri, jamur, kapang, ganggang dan tanaman hijau serta pada krustasea salah satunya yaitu pada kepiting Ozius sp. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Ozius sp. betina dengan metode KLT yaitu: β- karoten, Kantasantin, dan β- kriptosantin.Kata Kunci: Ozius sp, Kromatografi Lapis Tipis, Pigmen Karotenoid
ISOLASI PIGMEN KAROTENOID PADA KEPITING Metopograpsus sp. BETINA Franciskus K.I. Manik; Darus J Paransa; Desy MH Mantiri; Elvy L Ginting; Veibe Warouw; Rudy Moningkey
JURNAL PESISIR DAN LAUT TROPIS Vol. 8 No. 1 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.1.2020.27494

Abstract

Metopograpsus sp is one of species of crabs usually found in indo-pacifik and lived at mangrove's trunk. The characteristic of this type of crab has type color on the carapace organ that indicate the presence of carotenoid. carotenoid pigments are natural dyes in plants and crustacea. The purpose of this study is to determined type of carotenoid pigments of metopograpsus sp by using TLC Method. The result of this study were identified three type of pigments of metopograpsus sp that are β-Karoten, Astaxanthin, dan Astacene.Key word : Metopograpsus sp, TLC, Carotenoid pigments.AbstrakMetopograpsus sp adalah kepiting mangrove yang biasanya ditemukan di Indo-Pasifik dan hidup dibatang pohon mangrove. Ciri khas dari kepiting ini memiliki corak berwarna pada karapasnya sehingga diasumsikan kepiting tersebut mengandung pigmen karotenoid. pigmen karatenoid adalah pewarna alami yang terdapat pada tanaman dan hewan krustasea. Tujuan dari penelitian ini untuk mengetahui jenis-jenis pigmen karotenoid pada kepiting Metopograpsus sp dengan menggunakan metode KLT. Hasil penelitian terdapat tiga jenis pigmen karotenoid yang teridentifikasi pada kepiting Metopograpsus sp yaitu : β-Karoten, Astaxanthin, dan Astacene.Kata Kunci : Metopograpsus sp, KLT, pigmen karotenoid
Efek Senyawa Timbal Asetat Terhadap Pertumbuhan Dan Kandungan Pigmen Klorofil Mikroalga Dunaliella sp. Joshep Tamalonggehe; Kurniati Kemer; Darus Sa’adah J Paransa; Desy MH Mantiri; Nickson J Kawung; Suzanne L Undap
JURNAL PESISIR DAN LAUT TROPIS Vol. 8 No. 2 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.2.2020.28764

Abstract

Microalgae is one of the marine biota that has an important role in the waters because it acts as a supplier of food in the waters. Microalgae is a biological source that needs to be exploited because it is rich in essential compounds. Dunaliella sp. is one of the many micro algae used as research. Utilization of Dunaliella sp. quite diverse and has been marketed in developed countries because of its very attractive economic value. This study aims to determine the effects of lead acetate compounds on growth and content of chlorophyll pigments microalgae Dunaliella sp. The results obtained in this study are the lead acetate compounds can affect the number of cells in the growth of microalgae and analysis results obtained with a spectrophotometer showed that the extraction concentration of control day 5 (Exponential Phase) was higher than the concentration of 15 ppm and 25 ppm, whereas extraction on day 21 (Death Phase) concentration of 15 ppm was higher than 25 ppm.Keywords : Dunalella sp., Lead Acetate, Pigment chlorophyll RingkasanMikroalga adalah salah satu biota laut yang memiliki peran penting di perairan karena berfungsi sebagai pemasok makanan di perairan. Mikroalga adalah sumber biologis yang perlu dieksploitasi karena kaya akan senyawa esensial. Dunaliella sp. adalah salah satu dari banyak mikroalga yang digunakan sebagai penelitian. Pemanfaatan Dunaliella sp. cukup beragam dan telah dipasarkan di negara maju karena nilai ekonominya yang sangat menarik. Penelitian ini bertujuan untuk mengetahui efek senyawa timbal asetat terhadap pertumbuhan dan kandungan pigmen klorofil Dunaliella sp. Hasil yang diperoleh dalam penelitian ini adalah senyawa timbal asetat dapat mempengaruhi jumlah sel dalam pertumbuhan mikroalga, penurunan jumlah sel mengikuti konsentrasi timbal asetat yang diberikan dan hasil analisis yang diperoleh dengan spektrofotometer menunjukkan bahwa konsentrasi ekstraksi kontrol hari 5 (Fase Eksponensial) lebih tinggi daripada konsentrasi 15 ppm dan 25 ppm, sedangkan ekstraksi hari 21 (Fase Kematian) konsentrasi 15 ppm lebih tinggi dari 25 ppm.Kata kunci : Dunaliella sp., Timbal Asetat, Pigmen Klorofil
PENGARUH LOGAM BERAT TIMBAL TERHADAP PERTUMBUHAN ALGA Halimeda opuntia YANG DIKULTUR DALAM WADAH TERKONTROL Kristi Aji Sugiarto; Desy MH Mantiri; Nickson J Kawung; Rizald M Rompas; Sandra O Tilaar; Adnan Wantasen
JURNAL PESISIR DAN LAUT TROPIS Vol. 8 No. 3 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.3.2020.32239

Abstract

Heavy metal is a very dangerous pollutant waste for it cannot be destroyed (non-degradable) and over time it will accumulate in the ocean. This study aims to determine the lead content in sediment, water and algal thallus as well as to determine the algae growth that has been treated with lead. The research method used was a completely randomized design (CRD) which consisted of 3 treatments, using different treatments for each sample. The highest metal content obtained in the sediment was 5,124 ppm, in the water was 0.120 ppm, and in the algae thallus was 4,816 ppm respectively. The results showed that Halimeda opuntia algae can grow even in an environment contaminated with lead heavy metal and can become a bioremediation agent by absorbing lead heavy metal. Keywords: Halimeda opuntia, Lead Heavy Metal, Algae Culture, Bioremediation             Logam berat merupakan salah satu limbah pencemar yang sangat berbahaya karena tidak dapat dihancurkan (non degradable) dan dapat terakumulasi di perairan laut. Penelitian ini bertujuan untuk mengetahui kandungan timbal pada sedimen, air dan thallus alga yang dikultur serta mengetahui pertumbuhan alga yang telah diberi perlakuan timbal. Metode penelitian mengunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan, dengan menggunakan perlakuan yang berbeda pada tiap sampel dan kontrol. Kandungan logam tertinggi yang didapatkan pada sedimen yaitu sebesar 5,124 ppm, pada air sebesar 0,120 ppm, dan pada thallus alga sebesar 4,816 ppm. Hasil yang diperoleh yaitu alga Halimeda opuntia dapat bertumbuh walaupun dalam lingkungan yang tercemar logam berat timbal serta mampu menjadi agen bioremediasi dengan menyerap logam berat timbal. Kata kunci: Halimeda opuntia, Logam BeratTimbal, Kultur Alga, Bioremediasi
Ekstraksi Pigmen Klorofil Total Pada Mikroalga Dunaliella sp. Yang Telah diberi Perlakuan Timbal Asetat Oscar M Lamohamad; Kurniati Kemer; Desy M H Mantiri; Ester Angkow; James Paulus; Adnan S Wantasen
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 1 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.1.2021.33074

Abstract

Heavy metals such as lead are compounds that can cause toxic effects if they enter the body of living things. Dunaliella sp. is one of the marine organisms that is susceptible to changes or ecological pressure so that it is the main target of being exposed to pollutants such as heavy metals and so on, so it is necessary to conduct research to determine how the effect of lead acetate compounds on the growth and total chlorophyll content of Dunaliella micro algae. sp. The results of this study indicate that the growth of Dunaliella sp microalgae on the control culture media and the lead treatment media has a very different growth, the control media has normal growth, while the total chlorophyll content of the 14th day in the control media is 80.49 µg / ml. 30 ppm 54.79 µg / ml, 50 ppm 50.02 µg / ml and 100 ppm 9.13 µg / ml. While the total chlorophyll content of the 30th day in the control media was 34.99 µg / ml, 30 ppm 44.657 µg / ml, 50 ppm 26.136 µg / ml and 100 ppm 5.58 µg / ml.Keywords : Microalga, Dunaliella sp, kcorophyll, lead ecetate    
LAJU PERTUMBUHAN DAN KEPADATAN MIKROALGA Dunaliella sp. PADA PEMBERIAN TIMBAL ASETAT DENGAN KONSENTRASI YANG BERBEDA Fitly Tewal; Kurniati Kemer; Joice R.T.S.L. Rimper; Desy M.H. Mantiri; Wilmy E Pelle; Joppy D Mudeng
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 1 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.1.2021.33571

Abstract

Microalgae are organisms that contain chlorophyll and other pigments so they can carry out photosynthesis. Microalgae are widespread in nature and can be found in any environment exposed to sunlight. Microalgae are micro-sized biota with a diameter of less than 2 µm. The benefits of microalgae for other living things, especially humans, are numerous, including as a source of food and ingredients in the manufacture of medicines. Dunaliella sp. is a group of green algae that contains protein, fat and carbohydrates as a good source of food. Growth rate and density of microalgae Dunaliella sp. and the effect of lead acetate with different concentrations was observed using a microscope, starting from the lag phase, the logarithmic phase, the stationary phase and the declination phase. Dunaliella sp. Experiencing an exponential phase in the observation before treatment, namely on the 9th day and then doing the treatment. Treatment with lead acetate with concentrations of 10 ppm, 50 ppm and 80 ppm is very influential in the growth of microalgae. The result is that lead acetate contains toxins that can kill microalgae cells in both low and high concentrations.Keywords: Microalgae, Dunaliella sp., Lead Acetate, Concentration
KANDUNGAN LOGAM BERAT ARSEN PADA ALGA Kappaphycus alvarezii SERTA SEDIMEN DI PERAIRAN DESA BUKU KECAMATAN BELANG KABUPATEN MINAHASA TENGGARA Laurenzy Tampongangoy; Desy M H Mantiri; Darus SJ Paransa; Rizald M Rompas; James J H Paulus; suzanne Undap
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 2 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.2.2021.35514

Abstract

Kappaphycus alvarezii is one species algae that is very widely developed in the tropical waters, especially in Indonesia. The potential in these waters may decrease due to heavy metal pollution that accumulates in algae and sediments. Analyzing the content of heavy metal arsenic (As), using the SNI 01-2896-1998 test method, namely the metal contamination test method in food. The results obtained from this study were samples of brown and green Kapapphycus alvarezii taken from week I-V detected containing heavy metal arsenic (As) ranging from 0.17 - 0.42 ppm for green algae and 0.12 - 0.37 ppm for brown algae. This As concentration does not exceed the maximum heavy metal limit, which is 1.0 ppm for the maximum limit for heavy metal contamination in food. The concentration of heavy metal in sediments is 1.12 ppm, not exceeding the quality standard in the Environmental Protection Agency (EPA), the maximum heavy metal limit for arsenic in sediment is below 10 mg/kg
Co-Authors , Esther D. Angkouw . Nasprianto Abdul Ajiz Siolimbona Abdullah, Ridha Adnan S Wantasen Adnan S. Wantasen Adnan Sjaltout Wantasen Adnan Wantasen Agustin Rustam Andika Muhammad Andreas Albertino Hutahaean Andreas Albertino Hutahaean Andreas Hutahaean Angkouw, Esther Angkow, Esther Angmalisang, Ping Antonius P Rumengan Antonius P Rumengan Antonius P. Rumengan Antonius P. Rumengan Antonius Rumengan Antonius Rumengan Antonius Rumengan Asthisa, Dias Aswan Thamin August Daulat Balaira, Greisela Batar Siahaan Batarogoa, Nego E. Bawias, Miranti Billy J. Kepel Billy T. Wagey Boneka, Farnis Bineada Budi Kurniawan Calvyn F. A. Sondak, Calvyn F. A. Chatrien A. L. Sinjal Cyska Lumenta Darus S J Paransa Darus S. Paransa Dedy Octavian Siahaan, Dedy Octavian Deiske A. Sumilat Deiske Adeliene Sumilat, Deiske Adeliene Devi D. Suryono Dias Diasasthisa Djakatara, Paratiti Dewi Eko S Wibowo Elvi L. Ginting Elvy L Ginting Elvy L Ginting Engel V. Pandey Erly Y. Kaligis, Erly Y. Ester Angkow Farnis B Boneka Farnis B. Boneka Farnis Bineada Boneka Fatmawati Amin Filemon Hosea Firdaut Ismail Fitje Losung Fitly Tewal Franciskus K.I. Manik Frans Lumoindong Frans Lumoindong, Frans Fransine B. Manginsela Fransiscus Rori Gaspar D. Manu, Gaspar D. Grace Simanjuntak, Grace Grevo S Gerung Grevo S Gerung Grevo S. Gerung Haryani Sambali Hengky Manoppo Henky Manoppo Ilaria, Christabella Louisa Inayah Inayah Irmalita Tahir James J H Paulus James J H Paulus James J.H Paulus James JH Paulus James Paulus Jeheskiel, Aprilisa Viony John L. Tombokan Joice R.T.S.L Rimper Joice R.T.S.L. Rimper Joice Rimper Joppy D Mudeng Joppy D Mudeng Joppy Mudeng Joshep Tamalonggehe Joshian N.W. Schaduw Joshian Nicolas William Schaduw Julia L. Kalalo Juliaan Ch. Watung Jumeini, Jumeini Kadang, Nurfadillah Kawung, Nikita Khesyia A. Makhas Khreekhoff, Renny Khusnul Hotimah Kreckhoff, Reni L. Kristi Aji Sugiarto Kumampung, Deislie Kumampung, Deislie R. H. Kurnia Kemer, Kurnia Kurniati Kemer Kurniati Kemer Kurniati Kemer Kurniati Kemer Kurniati Kemer Kurniati Kemer Kurniati Kremer Lauluw, Dafid Laurenzy Tampongangoy Leibo, Regina Lepa, Bryan Gabriel Letha L. Wantania Lidya Magdalena Lintang, Rosita AJ Mamangkey, Noldy Gustaf Frans Mamuaja, Jane M. Mantiri, Rose Mantiri, Rose O.S.E. Marina F. O. Singkoh Mariska Astrid Kusumaningtyas Mariska Astrid Kusumaningtyas Markus T. Lasut Marpaung, Renaldy Alexander Medy Ompi Medy Ompi Meiske Salaki Muhammad Aris N. Gustaf F. Mamangkey Najamuddin N Nasir Sudirman Nasprianto Nasprianto Nasprianto, - - Nasprianto, . Natalie D Rumampuk Natalie D Rumampuk Natalie D. C. Rumampuk Natalie D.C Rumampuk Natalie Rumampuk, Natalie Nebuchadnezzar Akbar Nickson J Kawung Nickson J. Kawung, Nickson J. Novi Susetyo Adi Ockstan Jurike Kalesaran Ockstan Kalesaran Ockstan Kalesaran Oscar M Lamohamad Paraeng, Pricilia Paransa, Darus S.J. Paransa, Darus Sa'adah Johanis Paransa, Darus Saadah J. Pasodung, Aditya Paulus, James Paulus, James J. H. Pelafu, Widya Pelle, Wilmy Pelle, Wilmy E. Pelle, Wilmy Etwil Pratiwi Bone Puasa, Elsian Raja Doli Reiny A. Tumbol Reiny Tumbol Rene C. Kepel, Rene C. Rene Ch. Kepel Rene Ch. Kepel Rene Charles Kepel, Rene Charles Restu Nur Afi Ati Restu Nur Afi Ati Restu Nur Afi Ati Rignolda Djamaluddin Rizald M Rompas Rizald M Rompas Rizald M Rompas Rizald Max Rompas, Rizald Max Robert A. Bara Robert Bara Roeroe, Kakaskasen Andreas Rompas, Rizal M. Rompas, Rizald Rompas, Rizald M Rompas, Rizald M. Rosita Lintang Rudy Moningkey Rumengan, Anton Rustam Effendi Paembonan Salawati, Vellysa Friendly Sammy Londong Sammy N.J. Longdong Sanadi, Troy Sandra O Tilaar Sandra O. Tilaar Sandra Tilaar Sanep, Jacqlien Virgina Sarif Hidayat, Sarif Sinjal, Annita Ch. Sipriana S Tumembouw Sipriana Siana Tumembow Sipriani Tumembow Sri Winisari Van Gobel Sriyati Enjelina Sibarani Stenly Wullur Suria Darwisito, Suria Susan M. Sumampouw Suzanne L Undap Suzanne L Undap Suzanne L. Undap suzanne Undap Tamrin Tamrin Tamrin Taufiq Abdullah Terry Louise Kepel Terry Louise Kepel Terry Louise Kepel Tilaar, Ferdinand F. Tumalun, Abigael Joanete Veibe Warouw Veibe Warouw Veibe Warouw Victoria N Manoppo Wahidin, Nurhalis Wantah, Monika M. Werianty Liony Zeak Wilmy E Pelle Winda Mercedes Mingkid Yanti Kaleb, Yanti Yusmiana Puspitaningsih Rahayu Zulhan A Harahap