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PROLIFERASI DAN DIFERENSIASI SEL TULANG TIKUS DALAM MEDIUM KULTUR IN VITRO YANG MENGANDUNG EKSTRAK BATANG Cissus quadrangula Salisb. (SIPATAH-PATAH) Djuwita, Ita; Pratiwi, Irma Amalia; Winarto, Adi; Sabri, Mustafa
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.295

Abstract

Penelitian mengenai pengaruh ekstrak batang Cissus quadrangula Salisb. (sipatah-patah) terhadap tingkat proliferasi dan diferensiasi sel-sel tulang tikus (Sprague Dawley) prepuber umur empat minggu menggunakan sistem kultur in vitro. Sel-sel tulang dikultur dalam medium dulbeccos modified eagles medium (DMEM) yang diberi tambahan newborn calf serum (NBCS) 10%, non essential amino acid (NEAA) 10%, NaHCO3, ITS 1 l/ml (mengandung insulin 5 g/ml, transferin 10 g/ml, selenium 5 g/ml; Sigma St Louis USA), dan 50 g/ml gentamisin (mDMEM), dengan dan tanpa ekstrak Cissus quadrangula (CQ). Penelitian terdiri atas lima perlakuan yakni kontrol positif (mDMEM + deksametason 10-8 M), kontrol negatif (mDMEM), dan tiga konsentrasi CQ: mDMEM + CQ 0,3 mg/ml; mDMEM + CQ 0,6 mg/ml; dan mDMEM + CQ 1,2 mg/ml. Kultur dilakukan dalam inkubator CO2 5%, pada suhu 37 C selama tujuh hari. Parameter yang diamati adalah konsentrasi sel, komposisi, diameter osteoblas, dan diameter osteosit. Konsentrasi sel dihitung menggunakan hemositometer Newbauer. Komposisi osteoblas dan osteosit ditentukan berdasarkan pengamatan morfologi di bawah mikroskop cahaya. Diameter sel diukur menggunakan eyepiece micrometer. Data dianalisis menggunakan analisis varians dan uji Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak Cissus quadrangula Salisb. pada konsentrasi 0,3 mg/ml; 0,6 mg/ml; dan 1,2 mg/ml secara signifikan dapat meningkatkan proliferasi sel tulang; dan pada konsentrasi 0,6 mg/ml mampu menginduksi diferensiasi osteoblas menjadi osteosit (P0,05). Disimpulkan bahwa pemberian ekstrak Cissus quadrangula pada konsentrasi 0,6 mg/ml ke dalam medium kultur dapat meningkatkan proliferasi dan diferensiasi osteoblas.
Growth performance of gouramy (Osphronemus gouramy) with the addition of activated charcoal from tuna (Thunnus sp) bone waste in feed Nazlia, Suraiya; Nurhayati, Nurhayati; Riski, Ade Maya; Aprita, Ika Rezvani; Sabri, Mustafa; Afriana, Safrida; Febri, Suri Purnama
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 10: No. 1 (April, 2023)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v1i2.9431

Abstract

This study aims to determine the effect of adding activated charcoal from tuna bones on the growth of gouramy. This research was conducted at the Integrated Laboratory of the Faculty of Fisheries, Abulyatama University, Aceh. The test fish used were gouramy seeds with a size of 2-3 g and 5-6 cm. This study used a non-factorial completely randomized design, consisting of four treatments and three replications, including A (control; without the addition of activated charcoal), B (addition of 1% activated charcoal in feed), C (addition of 2% activated charcoal in feed), and D (addition of 3% activated charcoal in feed) for 60 days of the rearing period. Fish are kept in an aquarium with a size of 60 x 40 x 40 cm as many as 10 fish per container with a water level of 30 cm. During the maintenance period, fish were fed twice a day at satiation. Parameters measured included survival, absolute weight and length growth, daily growth rate (LPH), and feed conversion ratio (FCR). The data were analyzed using analysis of variance (ANOVA). if significantly different, continue further testing using Duncan. The results showed that the addition of 2% tuna bone activated charcoal in feed affected growth performance including in absolute weight and length growth, SGR, and gave the best value on FCR and SR of 8.98 ± 0.20 g; 3.36±0.13cm; 2.60±0.11; 1.63; 100±0.00%.Keywords: activated charcoal; Osphronemus gouramy; growth; fish bone
Herbal flavonoids in preserving testicular physiology under oxidative stress: A bibliometric analysis of systematic reviews Zulkifli, Baidillah; Fakri, Fajar; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Sabri, Mustafa; Lubis, Triva Murtina; Zainuddin, Zainuddin; Zulfitri, Zulfitri; Admi, Masda; Syah, Nanda Yulian; Usrina, Nora; Fajri, Fauzan
The International Journal of Tropical Veterinary and Biomedical Research Vol 8, No 2 (2023): Vol. 8 (2) November 2023
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v8i2.36616

Abstract

Herbal antioxidants have gained attention recently as possible treatments for disorders of male reproductive function linked to oxidative stress because of their ability to neutralize free radicals.The investigation into herbal flavonoids for preserving oxidative stress-impaired testicular physiology has been effectively conducted through the visualization of bibliometric analysis. Bibliometric data of published papers collected from the PubMed and Scopus databases in 2018-2023. Network visualization was performed using VOSviewer software on the set keywords that occur (n=15). The literature review was conducted according to the research trends identified by the clusters that appeared in the network visualization. The result revealed that a total of 266 records have been published based on the specified search set keywords since 2018. Through the analysis of keywords, inclusion and exclusion criteria, and the relevant content of papers, 11 articles were identified as suitable for conducting a literature review. Network visualization revealed that the research falls into two general categories: (1) toxicity to the testicles caused by oxidative stress and (2) prevention of oxidative stress impact on tissue through herbal medicine. Examination of the paper's content revealed that certain antioxidant compounds from plants can stabilize free radicals in the testicles. These antioxidants include vitexin, diosmin, hesperidin, phenolic acids, naphthoquinones, fisetin, pachypodol, sciadopitysin, carvacrol, and morin. However, further research is needed to compare active antioxidant content in different herbal plants and identify optimal concentrations for therapeutic effects.
English Vocabulary Size of Indonesian High School Graduates: Curriculum Expectation and Reality Mustafa, Faisal
IJELTAL (Indonesian Journal of English Language Teaching and Applied Linguistics) Vol 3, No 2 (2019): Indonesian Journal of English Language Teaching and Applied Linguistics
Publisher : Universitas Islam Negeri Sultan Aji Muhammad Idris Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21093/ijeltal.v3i2.278

Abstract

Vocabulary is most important in learning a language, and thus it should be taught before teaching other language skills. Teachers need to plan which words to teach to make students learn vocabulary easier; however, Indonesian high school curriculum does not specify which vocabulary to teach. Therefore, this research was aimed at finding out the list of vocabulary required by the curriculum of junior and senior high schools in Indonesia and whether they have learned those words after graduating high school levels. Vocabulary used in texts in the national examinations is interpreted as the curriculum-expected vocabulary; therefore, the data for curriculum expectation were collected from previous national examinations. The vocabulary was classified based on most-frequently used vocabulary provided by Nation (2006) with 14,000 word-families categorized into 14 levels. Only the first 5 levels (1st - 5th 1,000) were used in this research. Later, 1,775 high school fresh graduates were tested with this vocabulary to predict the percentage of the vocabulary they have learned. The results showed that the expected vocabulary size of junior high school graduates was the 1st 2,000 words and the 1st 3,000 words for senior high school graduates. Compared to the graduate predicted vocabulary size, they only had 72% vocabulary in the 1st 1,000, 54% in the 2nd 1,000, 49% in the 3rd. These results suggest that the graduate vocabulary size has not satisfied the curriculum expectation; therefore, Indonesian high school students should be taught all words in the first 3,000 list to be able to comprehend texts in the national examination.
Co-Authors Abdullah Hamzah, Abdullah Adi Winarto Afif Yuda Kusuma Afriana, Safrida Ali, Ihfan Khaidir Amiruddin Amiruddin Ayu Mentari Sarani Bina Aziz, Zulfadli A Burhanuddin Yasin Cut Erika Ramadhana Cut Mukramah Dian Masyitha Diana Fauzia Sari Elviwirda Elviwirda Endang Komariah Endang Tri Rahayu Erdiansyah Rahmi Erwin E Etriwati E Fajar Fakri Fajri, Fauzan Febri, Suri Purnama Fitria, Dinda Gani, Fadli A Gholib Gholib, Gholib Ginta Riady Hamny Hamny Hamny Sofyan Hasan, Denny Irmawati Hennivanda, Hennivanda Ika Apriani Fata Ika Rezvani Aprita Irma Amalia Pratiwi ITA DJUWITA Khabri, Ihsanul Khairil Khairil Kusdiantoro Mohamad M Nur Salim, M Nur M. Jalaluddin, M. M.iSA - Masda Admi Muhammad Hambal Muhammad Iqbal Muhammad Jalaluddin, Muhammad Muhibbuddin ., Muhibbuddin Mulyadi Adam Mulyadi Adam, Mulyadi Muslim Akmal Nanda Marlina Abdul Samad Nanda Yulian Syah Nazlia, Suraiya Nora Usrina Nur Inda Rahayu Nurhayati Nurhayati Nurzalika, Siti Omar K Burhan Putri Nurul A’la QURRATU AINI Razali Daud Rini Andriani Rinidar Rinidar, Rinidar Riski, Ade Maya Ritonga, Mudhita Zikkrullah Ritonga, Mudhita Zikrullah Rob Amery Rosmaidar Rosmaidar, Rosmaidar Rusli Rusli Safrida Safrida Samingan Samingan Siti Aisyah Sri Wahyuni Sugito - Syukriah ., Syukriah Tanjung, Fitri Aisah TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufan Hidayat Triva Murtina Lubis Tutiliana ., Tutiliana Ummu Balqis Wahono Esthi Prasetyaningtyas Wardhani, Lailia Dwi Kusuma Yenni Yusriani Yulia, Miga Zainuddin, Zainuddin Zulfadli A. Aziz Zulfadli A. Aziz Zulfitri Zulfitri, Zulfitri Zulkifli, Baidillah