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All Journal Jurnal Penelitian Saintek Agrikultura Journal of Tropical Soils Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Tanaman Industri dan Penyegar JURNAL AGROTEKNOLOGI Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Agroekoteknologi AGRICOLA Jurnal Agro Kultivasi JURNAL GALUNG TROPIKA Agrin : Jurnal Penelitian Pertanian Jurnal Pertanian Agros Jurnal Agrotek Tropika Paspalum: Jurnal Ilmiah Pertanian JURNAL PANGAN J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Agrotek Indonesia Jurnal Hexagro AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Jurnal ABDINUS : Jurnal Pengabdian Nusantara Indonesian Journal of Community Services Cel Journal of Tropical Mycorrhiza Jurnal Agronomika Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat
Anne Nuraini
Fakultas Pertanian Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Public Health Social Sciences Veterinary Other

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Pengaruh Pemberian Agen Hayati Pada Benih Dan Pupuk Bokashi Terhadap Mutu Fisiologis Benih Kedelai (Glycine max L. (Merill) Kultivar Grobogan Eka Siti Windia; Sumadi Sumadi; Anne Nuraini
Agrologia Vol 7, No 1 (2018): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v7i1.354

Abstract

Physiological quality of seeds could be seen from viability and vigor. The used of matriconditioning treatment using biological agent and bokashi fertilizer could be the way to enhance physiological quality of seeds. The objective of experiment was to determind the most effective dosage interaction of biological agent and bokashi fertilizer to improve physiological quality of seeds.This research was held at Ciparanje Experimental Farm and Laboratory of Seed Technology Faculty Agriculture Universitas Padjadjaran.from November 2016 until February 2017.  The experiment’s method design using randomized block design factorial pattern with Duncan’s Multiple Range Test (DMRT) at 5 %. The Cultivar that used for this research was Grobogan with Bokashi that tested at 0 ton/ha, 16 ton/ha, 32 ton/ha, and 48 ton/ha respectively combined with matriconditioning that used biological agent and control without biological agent. The biological agent that used for this treatment were Trichoderma spp., Azotobacter spp., Trichoderma spp + Azotobacter spp., with three replication.  The result of the experiment showed that interaction of biological agent and bokashi fertilizer affected the weight of 100 seeds and vigor index. The treatment that used Trichoderma spp. + Azotobacter spp. and bokashi fertilizer 48 ton/ha has showed that this dosage was the best for weight of 100 seeds parameter. Thus the treatment with trichoderma and azotobacter without bokashi fertilizer showed that this treatment affected the best for vigor index.Keyword: Soybean seeds, physiological quality, Trichoderma spp., Azotobacter spp., Bokashi.
Pemanfaatan Trichoderma harzianum dan biochar untuk mengatasi cekaman kekeringan pada kedelai fase reproduktif Benang - Purwanto; Indrawati - Indrawati; Sumadi Sumadi; Anne - Nuraini; Mieke Rochimi Setiawati
Jurnal Agro Vol 9, No 2 (2022)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/20684

Abstract

Pemanfaatan Trichoderma harzianum dan biochar sekam padi diharapkan mampu mengurangi dampak negatif cekaman kekeringan pada tanaman kedelai fase reproduktif. Tujuan penelitian ini untuk mengetahui interaksi aplikasi Trichoderma harzianum dan biochar sekam padi terhadap fisiologis dan hasil kedelai tercekam kekeringan selama fase reproduktif pembentukan polong (R3) sampai perkembangan biji (R6). Penelitian ini menggunakan rancangan split-split plot. Petak utama adalah cekaman air (75%, 50% dan 25% dari kapasitas lapang. Anak petak yaitu aplikasi Trichoderma harzianum (tanpa aplikasi Trichoderma harzianum dan dengan aplikasi Trichoderma harzianum dosis 50 g kg-1 benih kedelai), sedangkan anak-anak petak yaitu dosis biochar sekam padi (0, 5, dan 10 t ha-1). Hasil penelitian menunjukkan bahwa interaksi aplikasi Trichoderma harzianum 50 g kg-1 benih kedelai dan  biochar  sekam  padi dosis 10 t ha-1 secara fisiologis mampu meningkatkan kadar air relatif (KAR) daun, konduktansi stomata, sedangkan penurunan kadar prolin lebih dipengaruhi oleh adanya penambahan biochar dosis 5 dan 10 t ha-1.ABSTRACTThe use of Trichoderma harzianum and rice husk biochar is expected to reduce the negative effects of drought stress on soybean plants in the reproductive phase. The purpose of this study was to determine the interaction of the application of Trichoderma harzianum and rice husk biochar on the physiology and yield of drought-stressed soybeans during the reproductive phase of pod formation (R3) to seed development (R6). This study uses a split-split plot design. The main plots were water stress (75%, 50%, and 25% of field capacity. The subplots were the application of Trichoderma harzianum (without the application of Trichoderma harzianum and the application of Trichoderma harzianum at a dose of 50 g kg-1 soybean seeds), while the subplots were rice husk biochar doses (0, 5, and 10 t ha-1). The results showed that the interaction of the application of Trichoderma harzianum 50 g kg-1 soybean seeds and rice husk biochar dose 10 t ha-1was physiologically able to increase the relative water content (RWC) leaves, stomatal conductance, while the decrease in proline levels was more influenced by the addition of biochar doses of 5 and 10 t ha-1.
Induksi Kalus Eksplan Daun Stroberi (Fragaria x ananassa Duch) dengan Pemberian NAA dan CaP Secara In Vitro Fauzia Khaerunnisa Millenia; S Sumadi; Erni Suminar; Anne Nuraini; Gina Gustiani Pitaloka
JURNAL GALUNG TROPIKA Vol 11 No 3 (2022)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v11i3.1023

Abstract

The use of high-yielding strawberry varieties such as Sweet Charlie needs to be followed by proper propagation methods to produce large amounts of strawberry seeds quickly and free from disease. In vitro propagation techniques can be done through the formation of a callus. This study aims to determine the effect of the combination of NAA and CaP treatment on the in vitro induction of the Sweet Charlie strawberry variety leaf callus. This experiment was arranged using a completely randomized design (CRD) which consisted of 9 treatments, namely concentrations of NAA (0 mg L-1, 5 mg L-1, 10 mg L-1) and CaP (0 mgL-1, 1 mgL-1, 2mg L-1) which was repeated three times. The results showed that using NAA 5 mgL-1 was the best treatment to support callus induction of the Sweet Charlie strawberry variety in vitro. The supporting with the parameter percentage of callus formation reaching 100%, callus appearance time at 13.5 days after planting (DAP), callus diameter of 9.00 mm, callus weight of 0.52 g, and the percentage of crumbly textured callus was 50%.
Effect of magnetic field and arbuscular mycorrhizal fungi on germination and growth of tomato (Lycopersicon esculentum Mill.) Anne Nurbaity; Anne Nuraini; Eleonora Agustine
Journal of Tropical Mycorrhiza Vol. 1 No. 1: April 2022
Publisher : Asosiasi Mikoriza Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.93 KB)

Abstract

Agricultural technology innovation such as application of low magnetic field and arbuscular mycorrhizal fungi (AMF) could improve growth of some vegetable plants, such as tomatoes.The objectives of the research were to determine: (1) the effect of low magnetic field treatment on germination of tomato seeds, and (2) the combined effect of mycorrhiza and magnetic field treatments on growth of tomato plants. The first experiment was conducted in the laboratory to test the germination of magnetized and non-magnetized seeds on different growing mediums. The second experiment was conducted in a screenhouse using Randomised Blocked Design with the treatments of exposure of magnetic field (0 and 0.2 mT) and AMF (Glomus sp., Gigaspora sp. and Combination of Glomus sp. and Gigaspora sp.). Both treatments were applied as pre-sowing seed treatments. Results revealed the exposure of seeds to magnetic fields increased the seedling germination percentage, especially in the treatments combined with Gigaspora sp. (90%). Further measurement of plant heights showed that magnetized seeds consistently showed a higher plant height compared to non-magnet treatments. Gigaspora sp. which applied either as single or in mixture with Glomus spp. have shown better effect than Gigaspora sp. alone on the growth of tomatoes
Pengaruh Sistem Tanam pada pertumbuhan dan hasil serta susut bobot Tanaman Kangkung (Ipomoea reptans poir)Multiplikasi tunas temu putih (Curcuma zedoaria) secara in vitro dengan Beberapa Konsentrasi Zat Pengatur Tumbuh Thidiazuron Mugayanti; Fenny Dewi Nuroktavianti; Anne Nuraini
AGRONOMIKA Vol 21 No 01 (2023): JURNAL AGRONOMIKA
Publisher : Universitas Islam Batik Surakarta

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Abstract

Temu putih (Curcuma zedoaria) merupakan tanaman herbal yang memiliki potensi untuk mengobati berbagai penyakit seperti kanker dan tumor. Saat ini hampir 94% bahan baku obat tradisional berasal dari dalam negeri, namun penyediaan bahan baku industri obat tradisional masih terbatas. Teknik kultur jaringan dapat digunakan sebagai alternatif untuk produksi bibit dalam waktu yang singkat dengan jumlah yang banyak. Thidiazuron memiliki potensi memacu pembelahan sel secara cepat pada sel yang bersifat meristematik sehingga dapat meningkatkan multiplikasi tunas. Penggunakan TDZ dengan konsentrasi yang tinggi dapat menyebabkan tunas kerdil dan penghambatan pada pembentukan akar. Tujuan penelitian ini ialah untuk mengetahui pengaruh serta memperoleh konsentrasi terbaik TDZ terhadap proliferasi tunas temu putih. Penelitian dilaksanakan di Laboratorium Kultur Jaringan, Fakultas Pertanian, Universitas Padjadjaran, yang dirancang dengan Rancangan Acak Lengkap yang terdiri dari 5 perlakuan diantaranya Kontrol, 0 ppm; 0.3 ppm; 0.6 ppm dan 0.9 ppm TDZ yang diulang sebanyak tiga kali. Hasil penelitian menunjukkan Pemberian zat pengatur tumbuh thidiazuron berpengaruh pada terhadap jumlah tunas, jumlah akar, jumlah daun dan tinggi planlet temu putih. Pemberian 0.6 ppm TDZ menujukkan pengaruh yang baik terhadap multiplikasi tunas temu putih.
PERBANDINGAN EFEK BAP DAN KINETIN TERHADAP LAJU MULTIPLIKASI STROBERI KULTIVAR SWEET CHARLIE Camelia Andriani; Muhamad Kadapi; Erni Suminar; Anne Nuraini
Jurnal Agroteknologi Vol 14, No 1 (2023): Agustus 2023
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v14i1.18824

Abstract

Seed propagation of strawberry by in vitro culture is needed to obtain high quality seeds of strawberry in large quantities. In in vitro culture, a large number of shoots is expected can be produced at the multiplication stage. Cytokinins type of PGR can be added to culture media to produce a large number of shoot. The focus of this research was to compare the effect of BAP and Kinetin affected Sweet Charlie strawberry multiplication. The experiment was located at the Seed Technology Tissue Culture Laboratory, Faculty of Agriculture, Padjadjaran University. The experimental design was a Completely Randomized Design (CRD) with two treatments of different types of cytokinin, namely Benzyl Amino Purine (BAP) and Kinetin at 1 mg L-1 on Murashige and Skoog (MS) basic media, each treatment was repeated four times. This study showed that BAP had a better effect than Kinetin on the number of shoots, number of leaves, and wet weight of strawberry Sweet Charlie cultivar. The addition of BAP was recommended over Kinetin to obtain the highest number of shoots, the highest number of leaves, and the highest wet weight of culture on strawberry Sweet Charlie cultivar.
In vitro multiplication of strawberry in various types and concentrations of cytokinins and auxins Suminar, Erni; Rahmawati, Vira; Sumadi, Sumadi; Nuraini, Anne
Kultivasi Vol 23, No 1 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i1.42727

Abstract

Growth regulator is one of the important factors in the success of plant propagation by tissue culture. 6-Benzylaminopurine (BAP) is one of the most widely used cytokinin because it is more stable, less expensive and more effective. Thidiazuron (TDZ) can increase the action of other cytokinins, either exogenous cytokinin or endogenous cytokinin. 1-Naphthaleneacetic acid (NAA) belongs to the more stable auxin group, because it is not easily decomposed and oxidized by enzymes released by plants. This study aimed to obtain the best types and concentrations of BAP, TDZ, and NAA for the in vitro multiplication of strawberry explant. The experiment was conducted at the Seed Technology and Tissue Culture Laboratory, Faculty of Agriculture, Universitas Padjadjaran, from April to July 2022. The explants used were axillary shoots of strawberry var. Sweet Charlie is derived from plantlets that were propagated in vitro. The experimental design used was a completely randomized design with six treatments and four replications. The media used were Murashige dan Skoog (MS) with the addition of plant growth regulators (PGRs) in the form of BAP (0 ppm and 0,5 ppm), TDZ (0 ppm and 0,1 ppm), and NAA (0 ppm and 0,1 ppm). The application of different types and concentrations of BAP, TDZ, and NAA resulted in different effects of in vitro shoot multiplication of strawberry explant var. Sweet Charlie. Concentration of 0.1 ppm TDZ produced the highest number of shoots (5.38).
Response of tomato seed germination to several extraction techniques and magnetic field exposure treatments Shakina, Visira Deva; Nuraini, Anne; Nurbaity, Anne
Kultivasi Vol 23, No 1 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i1.44455

Abstract

The superior seeds are indicated by clean, good germination rate, pest and disease-free. One of the problems in providing superior tomato seeds is the presence of pulp attached to the tomato seeds, leading to less clean of seed. To remove the muscus on tomato seeds, the seeds have to be extracted. Other than seeds extraction, seeds exposure also could optimize seeds growth by increasing the metabolism of the tomato. The aim of this research is to determine the best extraction technique and magnetic field exposure for obtaining high tomato seeds germination. The complete random design used in this research consist of two factors, seeds extraction and magnetic field with three time replications. The result showed that seeds extraction for 24 hours is the best treatment for improving the maximum germination percentage, growth potential, growth rate, speed growth, uniformity index than others. Magnetic exposure at 6mT is the best treatment to improve seeds maximum growth and seeds speed growth.
Growth optimization of white turmeric (Curcuma zedoaria) plantlets with growth regulators gibberellins Murgayanti, Murgayanti; Nuroktavianti, Fenny Dewi; Nuraini, Anne
Kultivasi Vol 22, No 1 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i1.39163

Abstract

 The addition of cytokinin to the multiplication of white turmeric (Curcuma zedoaria) seedlings in vitro proved effective in shoot multiplication, but the addition of high cytokinin concentrations could cause stunted shoots and stunted growth. The addition of the hormone gibberellins (GA­3­) is often used in tissue culture for shoot elongation, so increasing the viability of plantlets. The experiment aims to determine the effect of GA3  and obtain the best concentrations of GA3 consisting of 0.50, 1, and 1.5 ppm GA3 on optimizing the growth of zedoary plantlets. The experimental parameters included the number of shoots, roots, leaves, plantlet height, and chlorophyll content observed at 6 WAP after subculturing. The results showed that giving 1 ppm GA3 had the best effect on optimizing plantlets' growth, namely the growth component of the number of shoots and plantlets' height.
The effect of fruit maturation stage on seed germination of iaa9-3 and iaa9-5 tomato mutants Al Aufa, Elfa Muhammad Ihsan; Mubarok, Syariful; Nuraini, Anne; Budiarto, Rahmat; Waluyo, Nurmalita; Ezura, Hiroshi
Kultivasi Vol 23, No 1 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i1.40859

Abstract

One of the internal factors that play an important role in seed quality is the level of the phytohormone auxin. Efforts to increase auxin in seeds include developing new varieties with increased indigenous auxin, such as those found in mutant tomatoes from Micro-Tom, namely iaa9-3 and iaa9-5. This research was conducted to determine the germination response of iaa9-3 and iaa9-5 mutant tomato seeds at different levels of the fruit maturation stage. The research was carried out at the Seed Technology Laboratory, Faculty of Agriculture, Padjadjaran University, and the Seed Testing Laboratory of the Center for Standard Testing of Vegetable Plant Instruments. The response design used was a completely randomized factorial design consisting of two factors and repeated three times. The first factor was the iaa9-3, iaa9-5, and Wild-Type Micro-Tom (WT-MT) mutants as controls. The second factor is the fruit maturation stage, which consists of Breaker, Pink, and Red. The research results showed that there was an interaction between genotype and fruit maturation stage on the parameters of germination, growth simultaneity, and hypocotyl length.  The harvest stage for tomatoes to produce normal strong seed germination was the pink stage in all tomato genotypes tested. The best germination rate was shown by WTMT seeds at the pink stage, genotype iaa9-3 at the red stage, and genotype iaa9-5 at the red or pink stage. The effect of fruit maturity stage on the synchronization of sprout growth was relatively not significantly different in mutant tomatoes, but it had an effect in WTMT tomatoes, namely the best pink stage.
Co-Authors Ade Ismail Ade Ismail Ade Ismail Ade Setiawan Adelia Anissa Putri Adinda Cikal Amalia Adinda Cikal Amalia Adinda Cikal Amalia, Adinda Cikal Aep Wawan Irwan Agung Karuniawan Agus Wahyudin Al Aufa, Elfa Muhammad Ihsan Alan Randall Alin Robiah Al Adawiyah Alvianto, Muhamad Amalia, Inneke Anindya, Marsya Nabila Anne Nurbaity Anni Yuniarti Annisa Nur Rahmani Annisa Nuraisah Annisa Nurul Fatimah Aprilia, Eva Argapati Sela Argapati Sela, Argapati Arin Rosmala Ariyanto, Nur Budi Arrin Rosmala Ashari, Asri Mulya Asri Peni Wulandari Asyifa Mardatillah Ayu Ratna Ningrum Azizah, Annisa Nanda Nur Bella Dian Ratnasari. Bella Dian Ratnasari., Bella Dian Benang Purwanto Camelia Andriani Christine Angel Citra Bakti, Citra Cucu Suherman Debby Yolanda Sulista Dedi Ruswandi Dedi Widayat Denny Sobardini Denny Sobardini Denny Sobardini Sobarna Denny Sobardini, Denny Diah Rochana Puspitasari Dian Ardiansyah Dian Ardiansyah Dikdik Kurnia Dirga Sapta Sara Donita, Yukta Alvira Edy Suryadi Ega Raisya Ega Raisya Eka Siti Windia Eko Wahyudi Eleonora Agustine Elma, Tiara Erizon, Meisyela Salsabila Erni Erni Erni Suminar Eva Aprilia Eva Aprilia Ezura, Hiroshi Farida Farida Damayanti Fatimah, Annisa Nurul Fauzia Khaerunnisa Millenia Fenny Dewi Nuroktavianti Fitri Widya Fitriatin Fitrianti Widya Lestari Ganjar Herdiansyah Gina Gustiani Pitaloka H. Apriyanto H. Apriyanto, H. Hapizhah Hapizhah Hapizhah Hapizhah, Hapizhah Herlistin Mooy Heru Waskito Heru Waskito Hiroshi Ezura Hiroshi Ezura Ibrahim, Meynarti Sari Dewi Indrawati - Indrawati Inneke Amalia Intan Ratna Dewi Anjarsari Intan Winara Iwan Setiawan Jajang Sauman Hamdani Khadamullah M Nurhuda Khadamullah Nurhuda Kusumadewi, Vira Kusumiyati Kusumiyati Luciana Djaya, Luciana M Kadapi M. Ibrahim M. Ibrahim, M. Marsya Nabila Anindya Masako Akutsu Meddy Rachmadi Megianti Agtari Meisyela Erizon Mia Munggarani Mieke Rochimi Setiawati Mira Ariyanti Mita Indriani Mochamad Arief Soleh Mohamad Arief Soleh Mugayanti Muhamad Alvianto Muhamad Kadapi Muhammad Abdillah Hasan Qonit Muhammad Syafii Murgayanti Murgayanti Neni Rostini Neni Rostini Niki Rahayu Noladhi Wicaksana Noor Istifadah Nur Azizah Romadhoni Nur Budi Ariyanto Nuraini, Hanifah Nurjanah Nurjanah Nuroktavianti, Fenny Dewi Nursuhud Nursuhud Nuzula Suci Azima Nuzula Suci Azima, Nuzula Suci Pangaribuan, Ikhwan Fadli Parlinah, Linlin Pujawati Suryatmana Qonit, Muhammad Abdilah Hasan R. Damayanthi R. Damayanthi, R. R. Pratama R. Pratama, R. Rahmani, Annisa Nur Rahmat Budiarto Rahmawati, Vira Raisya, Ega Ramadani, Selika Fitrian Ramadhani, Selika Fitrian Randall, Alan Randriani, Enny Rezeki Simamora Rika Meliansyah Rubaekah, Siti Sarah Rufaidah, Fathi S Sumadi Santika Sari Sari, Laela Shakina, Visira Deva Sheli Mustikasari Dewi SIska Rasiska, SIska Siti Julaeha, Siti Siti Rodiah Siti Sarah Rubaekah Siti Syarah Nurbaekah Suci Azima, Nuzula Sudarjat Sudarjat Sulistyaningsih Sulistyaningsih Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi SUMADI SUMADI Sumadi Sumadi Sumadi Sumadi Sunjaya Putra Syariful Mubarok TATI NURHAYATI Tati Nurmala Tati Nurmala Tiara Elma Tino Mutiarawati Tino Mutiarawati Wahyudin, Agus Waluyo, Nurmalita Warid Ali Qosim Wawan Sutari Yayat Rochayat Yayat Rochayat Suradinata Yayat Rochayat, Yayat Yudhistari Sihombing Yulianto, Fiky Yusti Yusti, Yusti Yuyun Yuwariah Yuyun Yuwariah Yuyun Yuwariah Zulfatunnisa Zulfatunnisa