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All Journal HAYATI Journal of Biosciences Vegetalika Jurnal Ilmu Pertanian Agrikultura Jurnal Ilmu Dasar Jurnal Natur Indonesia AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrovigor: Jurnal Agroekoteknologi Biogenesis: Jurnal Ilmiah Biologi CEMARA Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Planta Tropika UNEJ e-Proceeding Jurnal Penelitian Pertanian Terapan Agriprima : Journal of Applied Agricultural Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA) Agrin : Jurnal Penelitian Pertanian SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Agrotek Tropika AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA Jurnal Kreativitas PKM jurnal Ilmu Pertanian (Agricultural Science) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Agrotek Indonesia Agroteknika Agro Bali: Agricultural Journal Jurnal Pengabdian Masyarakat IPTEKS Jurnal Bioindustri Jurnal Pengabdian Masyarakat Sasambo BIOVALENTIA: Biological Research Journal Journal of Applied Agricultural Science and Technology Prosiding Seminar Nasional Pengabdian Kepada Masyarakat PAPUMA: Journal of Comunnity Services Biosaintifika: Journal of Biology & Biology Education Journal of Bioindustry Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Natur Indonesia
Restanto, Didik Pudji
Prodi Agronomi, Fakultas Pertanian, Universitas Jember
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Combination Effect of Culture Media and Silver Nanoparticle on the Effectiveness of Tobacco Anther Regeneration Nur Khozin, Mohammad; Ahnaf, Yusuf Dary; Dewanti, Parawita; Restanto, Didik Pudji; Iryono
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.681-693

Abstract

Anther culture is an effective biotechnological approach to accelerate the production of homozygous lines in tobacco breeding. This study evaluated the interaction between culture media (Murashige and Skoog/MS and Chu’s N6) and silver nanoparticle (AgNP) concentrations on in vitro anther regeneration of tobacco (Nicotiana tabacum L.). The experiment was arranged in a completely randomized design with ten treatment combinations consisting of two basal media and five AgNP concentrations (0, 2.5, 5, 7.5, and 10 ppm), each replicated three times. Quantitative parameters included callus formation, shoot regeneration, rooting response, regeneration percentage, and contamination rate. MS medium supplemented with 2.5 ppm AgNPs (K2) produced the optimal response, showing 100% callus formation and the highest shoot regeneration percentage (89%). Root formation was observed only in treatments K2 and K4. In contrast, higher AgNP concentrations (≥7.5 ppm), particularly in Chu’s N6 medium, significantly reduced regeneration percentages (ANOVA, p<0.05). The promotive effect of low AgNP concentration is associated with suppression of ethylene activity, antimicrobial action, and regulation of reactive oxygen species (ROS), which collectively enhance cell division and organogenesis. These findings indicate that MS medium supplemented with 2.5 ppm AgNPs is optimal for tobacco anther regeneration and suitable for doubled-haploid production.
Pendampingan masyarakat untuk pembentukan TABOGA Farm guna peningkatan kesejahteraan petani di desa Klangon kabupaten Madiun Alfarisy, Fariz Kustiawan; Restanto, Didik Pudji; Widuri, Laily Ilman; Witono, Yuli; Fajrin, Fifteen Aprila; Paramu, Hadi
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 8, No 2 (2024): June
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v8i2.22740

Abstract

AbstrakDesa Klangon saat ini menjadi desa rujukan tanaman porang Indonesia. Tidak hanya itu Desa Klangon memiliki wisata klangon yang terletak di tengah hutan klangon. Tujuan dari pengabdian masyarakat adalah memberikan pemberdayaan dan pendampingan masyarakat mengenai implementasi Agro Smart Village sebagai solusi pengelolaan wisata dan TABOGA dalam upaya peningkatan kemandirian dan derajat kesehatan masyarakat. Tahapan pelaksanaan kegiatan meliputi koordinasi dengan perangkat desa,  FGD (Focus Group Discussion) dan praktek di beberapa kelompok masyarakat, pendampingan, pembentukan TABOGA farm, dan pendampingan, Pendampingan dalam perencanaan pengembangan usaha hilirisasi produk TABOGA. Kegiatan pengabdian desa Asal melibatkan beberapa perangkat desa, tokoh masyarakat, dan petani yang menjadi sasaran. Hasil yang didapatkan dari kegiatan pengabdian ini diantaranya adalah komoditas TABOGA di desa klangon memiliki potensi yang besar untuk dikembangkan sebagai komoditas unggulan kedua setelah porang. Setidaknya dari hasil FGD tercatat sebanyak 18 jenis tanaman obat tradisional yang banyak tumbuh berdampingan dengan tanaman porang di hutan Desa Klangon. Namun, potensi ini belum banyak dimanfaatkan secara maksimal. Permasalahan utama yang menjadi kendala adalah pemasaran produk TABOGA. Berdasarkan permasalahan tersebut, kontribusi yang diberikan oleh Tim Pengabdi pada kegiatan ini yakni  pendampingan implementasi Agro Smart Village melalui pemetaan komoditas TABOGA untuk meningkatkan minat masyarakat dalam mengembangkan komoditas TABOGA untuk kesejahteraan masyarakat Desa Klangon. Kata kunci: pemasaran; porang; tanaman obat tradisional; wisata. AbstractKlangon Village is currently a reference village for Indonesian porang plants. Klangon Village has a Klangon tourist area which is in the middle of the Klangon forest. The purpose of community service was to provide community empowerment and assistance regarding the implementation of Agro Smart Village as a tourism management solution and TABOGA to increase self-sufficiency and community health status. The method of implementing the assisted village service activities was carried out using two methods, namely FGD (Focus Group Discussion) and practiced in several community groups. The production technology practice method aimed to aid the community regarding the formation of TABOGA Farm. Origin village service activities involved several village officials, community leaders, and targeted farmers. Based on the results of the community service activities, the Klangon people required assistance from universities to make mapping of TABOGA commodities and their marketing. Farmers need institutional support such as BUMDES to become a business unit that accommodates community taboga plant products so that it is hoped that they can increase people's income.Keywords: tourism; traditional medicinal plant; marketing.
Peningkatan Ketahanan Tanaman Kedelai (Glycine max (L.) Merril) Terhadap Penyakit Antraknosa Melalui Induksi Mutasi Sodium Azide Fathoni, Rizal Dwi; Hartatik, Sri; Restanto, Didik Pudji
Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Vol. 23 No. 2 (2025): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v23i2.4774

Abstract

Kedelai (Glycine max (L.) Merril) merupakan tanaman dengan kandungan nutrisi yang tinggi, tapi produktivitas kedelai cukup rendah, hal ini disebabkan oleh serangan penyakit Antraknosa. Penelitian ini bertujuan untuk meningkatkan ketahanan tanaman melalui induksi mutasi sodium azide. Percobaan dilakukan pada Februari 2021 - Juli 2022 di Greenhouse Jl. Prajurit Syakur, Kecamatan Srono, Kabupaten Banyuwangi. Percobaan ini dilakukan melalui beberapa tahapan yaitu; Perendaman benih kedelai varietas Wilis dan Grobogan dengan larutan sodium azide pada konsentrasi 0mM dan 0,663mM, Penanaman benih kedelai hasil induksi mutasi, Inokulasi Antraknosa, dan Pengamatan ketahanan tanaman. Data yang didapatkan dianalisis dengan menggunakan data deskriptif. Induksi mutasi sodium azide pada 0,663mM dapat meningkatkan ketahanan tanaman terhadap penyakit Antraknosa. Ketahanan tanaman dilihat dari persentase tingkat keparahan penyakit. Terdapat 2 kriteria tingkat keparahan penyakit yaitu Moderate (Wilis dan Grobogan 0mM, Grobogan 0,663mM) dan Resisten (Wilis 0,663mM).
The Effect of Media Composition on the Growth of Fig (Ficus carica L.) Grafts Didik Pudji Restanto; Moh Nuri Antono; Setiyono Setiyono; Wildan Muhlison; Mohammad Candra Prayoga; Fauziatuz Zahro
Journal of Applied Agricultural Science and Technology Vol. 9 No. 3 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i3.413

Abstract

The fig plant is cultivated for its fruit due to its high economic value. Vegetative propagation can serve as an alternative for mass propagation of fig plants, such as grafting. Vegetative propagation of figs is conducted in several ways, including grafting and cuttings. The problems encountered in grafting are mainly caused by unsuitable media conditions. This research aims to determine the best composition of grafting media for fig plants. The study was conducted in Dwiwangsa Tin Garden Park, Sidomulyo Village, Krian District, Sidoarjo Regency, East Java. Rainfall at the research site was classified as dry season, with 0 mm in October 2023, 7 mm in November 2023, 150 mm in December 2023, 211 mm in January 2024, and 485 mm in February 2024. The research employed a randomized block design (RBD) consisting of nine media combination treatments. The treatments included soil medium, husk medium, husk charcoal medium, cocopeat medium, sawdust sewing powder medium, a combination of soil and husk medium (1:1), a combination of soil and charcoal husk medium (1:1), a combination of  soil and cocopeat medium (1:1), and a combination of soil and sawdust medium (1:1). Each grafting medium showed a different growth response in terms of root number. Based on the results, the husk charcoal medium produced the highest values, with 89.8 roots, root length of 7.24 cm, root diameter of 1.38 cm, root fresh weight of 5.22 g, root volume of 7.7 ml, and root dry weight of 0.37 g.
Mycorrhizal Seed Coating Enhances Root Volume and Yield Components of Edamame Soybean Fariroh, Indri; Restanto, Didik Pudji
Jurnal Ilmu Pertanian Vol 11, No 1 (2026): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.105717

Abstract

Elevated osmotic pressure in the root zone under saline conditions acts as primary constraint to crop production by disrupting essential physiological growth process. One effective strategy for soil saline management involves the use of mycorrhizal inoculants. Delivering these microbes via seed coating is a streamlined, economical, and accurate approach that optimizes plant performance under diverse stress condition. This study aimed to evaluate the effect of mycorrhizal seed coating on the growth and yield of edamame under saline conditions. This research was arranged in a randomized complete block design with two factors, consisting of mycorrhizal seed coating (0 and 12 g mg-1) and planting media (soil, saline soil, soil + saline soil (1:1)). There were six treatment combinations, and each treatment was replicated four times, resulting in a total of 24 experimental units. The observed variables include plant height, root volume, number of pods per plant, weight of pod per fruit, number of seeds per plant, and weight of 100 seeds. The result showed that mycorrhizal seed coating had potential to improve edamame root development and optimize phosphorus uptake. While vegetative height remained unaffected, the treatment substantially improved yield components, including number of pods, seed weight, and overall seed weight. The efficacy of colonization is contingent upon soil pH and phosphorus availability, highlighting the importance of site-specific conditions. Consequently, mycorrhizal seed coating represents a practical and cost-effective strategy for improving edamame performance, offering a viable solution for enhancing crop resilience and yield in nutrient-deficient or saline environments.
Mycorrhizal Seed Coating Enhances Root Volume and Yield Components of Edamame Soybean Fariroh, Indri; Restanto, Didik Pudji
Jurnal Ilmu Pertanian Vol 11, No 1 (2026): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.105717

Abstract

Elevated osmotic pressure in the root zone under saline conditions acts as primary constraint to crop production by disrupting essential physiological growth process. One effective strategy for soil saline management involves the use of mycorrhizal inoculants. Delivering these microbes via seed coating is a streamlined, economical, and accurate approach that optimizes plant performance under diverse stress condition. This study aimed to evaluate the effect of mycorrhizal seed coating on the growth and yield of edamame under saline conditions. This research was arranged in a randomized complete block design with two factors, consisting of mycorrhizal seed coating (0 and 12 g mg-1) and planting media (soil, saline soil, soil + saline soil (1:1)). There were six treatment combinations, and each treatment was replicated four times, resulting in a total of 24 experimental units. The observed variables include plant height, root volume, number of pods per plant, weight of pod per fruit, number of seeds per plant, and weight of 100 seeds. The result showed that mycorrhizal seed coating had potential to improve edamame root development and optimize phosphorus uptake. While vegetative height remained unaffected, the treatment substantially improved yield components, including number of pods, seed weight, and overall seed weight. The efficacy of colonization is contingent upon soil pH and phosphorus availability, highlighting the importance of site-specific conditions. Consequently, mycorrhizal seed coating represents a practical and cost-effective strategy for improving edamame performance, offering a viable solution for enhancing crop resilience and yield in nutrient-deficient or saline environments.
Optimization of Somatic Embryogenesis Formation in Hoya (Hoya carnosa (L.) R. Br.) with Different Hormone Combinations Restanto, Didik Pudji; Prayoga, Mohammad Candra; Jaenuri, Ahmad; Avivi, Sholeh; Soeparjono, Sigit; Selopa, Tanaya Asmara Citra; Ullah, Rehan; Alfarisy, Fariz Kustiawan
AGRIVITA Journal of Agricultural Science Vol 48, No 2 (2026): IN PRESS
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.4844

Abstract

Hoya carnosa (L.) R. Br. propagation is conventionally less efficient, and the amount produced is low. This study aims to determine the influence of hormone type and concentration on changes in cell structure and the phase of somatic embryogenesis of the Hoya plant in vitro. The study was conducted in two stages, namely the first stage of callus induction with a combination of BAP (0.4 mg/l, 0.5 mg/l, 0.6 mg/l) and 2,4-D (5 mg/l, 6 mg/l, 7 mg/l) and the second stage of somatic embryogenesis with a combination of BAP hormones (0.5 mg/l, 1.0 mg/l, 1.5 mg/l) and IBA (0.25 mg/l, 0.5 mg/l, and 0.75 mg/l). The data were analyzed using ANOVA and further analyzed by using DMRT. Based on the results, the combination of BAP 0.4 mg/l and 2,4-D 7 mg/l hormones showed the best growth with the parameters of the appearance of callus at 23 days, callus weight 2.92 g, and embryogenic calluses formed with crumb texture and greenish-yellow color. The combination of BAP 1 mg/l and IBA 0.75 mg/l resulted in the frequency of budding emergence of 23.3 days and the number of tendrils 20.7. This treatment was able to produce 5 roots and 2 shoots.
Effect of Light Color and Auxin on Callus Induction and Development in Amorphophallus muelleri Blume. Popy Hartatie Hardjo; Michael Anthony Thongiratama; Fenny Irawati; Didik Pudji Restanto
Vegetalika Vol 14, No 3 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.106227

Abstract

Porang (Amorphophallus muelleri Blume) is a significant player in the international market, but its slow seedling growth rate is a major concern. This study, which holds significant implications for porang cultivation, aimed to accelerate the seedling growth phase through the induction of adventitious shoots from callus proliferation. We explored the use of plant growth regulators (PGRs), specifically auxins (NAA and 2,4-D) and cytokinins (BAP), in combination with variations in light color (white and white-blue). The observed parameters included the time of first callus and adventitious shoot emergence, callus growth, number of adventitious shoots, and callus coloration. Data were subjected to two-way analysis of variance (ANOVA) to evaluate the effects of plant growth regulator (PGR) type and light quality as independent variables. Statistical significance was assessed at a 95% confidence level using SPSS software. The combination of 1.0 ppm NAA and 0.5 ppm BAP in MS medium under white-blue light effectively accelerated callus induction from bulbil explants, with visible callus formation by day 8 and the fastest proliferation over 7 weeks. Under white light, the same PGR combination also promoted the earliest adventitious shoot formation by day 16 and yielded the highest number of shoots, totaling seven by the end of the culture period.
Analysis of the Application of Vitamin B1 on the Response of Salinity Stress Resistance in Several Varieties of Rice (Oryza Sativa L.) Tri Ratnasari; Tri Handoyo; Parawita Dewanti; Didik Pudji Restanto
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.6324

Abstract

One of the most common stresses in rice cultivation is salinity. Rice plants stressed by salinity exhibit changes such as yellowing leaves, drying tips, and chlorosis. The efforts made by the government and farmers so far include implementing cultivation scheduling techniques, planting patterns, and using stress-resistant varieties, as well as improving soil to increase water-holding capacity through lime application. Each of these efforts comes with its own risks. Another approach to enhance the growth and yield of rice plants is the application of vitamins. Providing vitamins can stimulate the growth of plant organs, as they play a crucial role in the growth process by acting as catalysts for metabolism. Research has indicated that vitamin B1 can significantly promote plant growth under stressful conditions. This study aims to investigate the positive effects of various concentrations of vitamin B1 on the growth and yield of rice plants while also reducing salinity stress. The method used involved planting three varieties of rice—IR-46, Inpari-32, and Pokkali—in planting buckets using the TABELA system. Vitamin B1 was applied at concentrations of 0, 5, and 10 mM during the peak vegetative phase, with salinity stress of 6 dS/m introduced one day after vitamin application. The plants were maintained under salinity stress conditions until harvest, during which morphological and phytochemical analyses were conducted. Morphological analysis included measurements of plant height, number of tillers, number of productive tillers, number of grains per panicle, and percentage of healthy grains. Biochemical parameters measured included total chlorophyll and electrolyte leakage analysis. The results indicate that vitamin B1 can effectively reduce stress in plants affected by salinity.
Co-Authors . Usmadi Adinda, Intan Dwi Agnes Natalia Wijaya Ahnaf, Yusuf Dary Aji, Seto Purnomo Alfarisy, Fariz Kustiawan Anang Syamsunihar Aprila Iga Mufidah Arsyika, Izna Arya Wiranegara Azizah, Kunti Anis Bambang Kuswandi Bambang Sugiharto Bintoro, Jatmiko Hardi Budha, Al Sura Tri Budi Kristanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto, Budi Calista, Zalza Candra Prayoga, Mohammad Darah Pertami, Rindha Rentina Dinata, Gallyndra Fatkhu Dodi Wijaya Dwi Erwin Kusbianto Dwika Nano Hariyanto Fanata, Wahyu Indra Duwi Farlisa, Veronenci Yuliarbi Fathoni, Rizal Dwi Fauziatuz Zahro Fenny Irawati Fenny Irawati Fifteen Aprila Fajrin Firdauzi, Sandy Al Firdha Narulita Alfian Hadi Paramu Haliza, Nurhayadatul Handayani, Etty Hanifah, Fairuz Luthfi HARDIAN SUSILO ADDY Harsanti, Restiani Sih Indraloka, Aldy Bahaduri Indri Fariroh Iryono Jaenuri, Ahmad Kacung Hariyono Ketut Anom Wijaya Khozim, Mohammad Nur Khozin, Mohammad Nur Kriswanto, Budi Kushariyadi Kushariyadi Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Luluk Noviana Lutfi Pramukyana Michael Anthony Thongiratama Mochammad Wildan Jadmiko Moh Nuri Antono Mohammad Candra Prayoga Mohammad Nur Khozim Mona, Muhammad Dima Say Muhammad Dima Say Mona Muhammad Nur, Kholid Rosyidi Munandar, Denna Eriani Murtaqib Murtaqib Nadiya, Nisma Riyadh Nafiah, Nur Lailin Nafisah Iqmatullah Natasha Florenika Nur Lailin Nafiah Nurfika Asmaningrum Nurhasna, Rifqi Pamungkas, Wahyu Eka Parawita Dewanti Parawita Dewanti Parawita Dewanti Poppy Hartatie Hardjo Popy Hartatie Hardjo Popy Hartatie Hardjo Prayoga, Mohammad Candra Putri, Widya Kristyanti Rahmawati Rahmawati Raisah Bani Ratnasari, Tri Refa Firgiyanto Refa Firgiyanto Resti, Ricke Windi Rizqi Maulana Rusdiana, Riza Yuli Saputro Gumelar, Bachtiar Selopa, Tanaya Asmara Citra Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Simamora, Endah Cahyani Slameto . Slameto Slameto Slameto Slameto Slameto Slameto Sri Hartatik Suliswanto, Eko Nur Sundahri, Sundahri Susanto, Arnis Budi Thohirin, Ahmad Nazmi Thongiratama, Michael Anthony Tri Agus Siswoyo Tri Handoyo TRI HANDOYO Tri Ratnasari Tri Rini Kusparwanti, Tri Rini Tri Widyastuti Ubaidillah, Mohammad Ullah, Rehan Ummi Solikhah, Ummi Vega Kartika Sari Veronenci Yuliarbi Farlisa Wahyu Indra Duwi Fanata Widya Kristiyanti Putri Widya Kristiyanti Putri Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Witono Zahro, Fauziatuz