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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Jurnal Ilmu Pertanian Jurnal Ilmu Lingkungan Agrikultura Jurnal Ilmu Dasar Jurnal Natur Indonesia AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrovigor: Jurnal Agroekoteknologi Biogenesis: Jurnal Ilmiah Biologi CEMARA Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Jurnal Agro Planta Tropika UNEJ e-Proceeding Jurnal Penelitian Pertanian Terapan Agriprima : Journal of Applied Agricultural Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA) Agrin : Jurnal Penelitian Pertanian SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Agrotek Tropika AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA Jurnal Kreativitas PKM jurnal Ilmu Pertanian (Agricultural Science) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Agrotek Indonesia Agroteknika Agro Bali: Agricultural Journal Jurnal Pengabdian Masyarakat IPTEKS Jurnal Bioindustri Jurnal Pengabdian Masyarakat Sasambo BIOVALENTIA: Biological Research Journal Journal of Applied Agricultural Science and Technology Prosiding Seminar Nasional Pengabdian Kepada Masyarakat PAPUMA: Journal of Comunnity Services Biosaintifika: Journal of Biology & Biology Education Journal of Bioindustry Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Natur Indonesia
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Analysis of the Application of Vitamin B1 on the Response of Salinity Stress Resistance in Several Varieties of Rice (Oryza Sativa L.) Ratnasari, Tri; Handoyo, Tri; Dewanti, Parawita; Restanto, Didik Pudji
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.6324

Abstract

One of the most common stresses in rice cultivation is salinity. Rice plants stressed by salinity exhibit changes such as yellowing leaves, drying tips, and chlorosis. The efforts made by the government and farmers so far include implementing cultivation scheduling techniques, planting patterns, and using stress-resistant varieties, as well as improving soil to increase water-holding capacity through lime application. Each of these efforts comes with its own risks. Another approach to enhance the growth and yield of rice plants is the application of vitamins. Providing vitamins can stimulate the growth of plant organs, as they play a crucial role in the growth process by acting as catalysts for metabolism. Research has indicated that vitamin B1 can significantly promote plant growth under stressful conditions. This study aims to investigate the positive effects of various concentrations of vitamin B1 on the growth and yield of rice plants while also reducing salinity stress. The method used involved planting three varieties of rice—IR-46, Inpari-32, and Pokkali—in planting buckets using the TABELA system. Vitamin B1 was applied at concentrations of 0, 5, and 10 mM during the peak vegetative phase, with salinity stress of 6 dS/m introduced one day after vitamin application. The plants were maintained under salinity stress conditions until harvest, during which morphological and phytochemical analyses were conducted. Morphological analysis included measurements of plant height, number of tillers, number of productive tillers, number of grains per panicle, and percentage of healthy grains. Biochemical parameters measured included total chlorophyll and electrolyte leakage analysis. The results indicate that vitamin B1 can effectively reduce stress in plants affected by salinity.
Water footprint of melon production under different nutrient and plant growth regulator management Rohman, Fadil; Kurniasari, Leli; Azizah, Maria; Firgiyanto, Refa; Sukri, M Zayin; Rohman, Hanif Fatur; Restanto, Didik Pudji; Tini, Etik Wukir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 3 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i3.60461

Abstract

Applying environmentally friendly technology in melon cultivation aims to create sustainable agriculture. Nutrient and plant growth regulators management are simple to apply and have a relatively rapid effect on melon fruit production. Water footprint assessment in melon cultivation is crucial for ensuring sustainable agricultural practices and efficient water use. This study aimed to evaluate the electroconductivity of nutrient solutions and GA3 concentration in increasing fruit production and water footprint efficiency. This research was conducted from June to September 2022 at Tefa SGH Polije. This research used a completely randomized design with treatments that consisted of nine combinations of nutrient solution electrical conductivities (2.8, 3.2, and 3.6 mS cm-1) and GA3 concentrations (0, 60, and 120 mg L-1). Observation variables were fruit diameter, edible part thickness, fruit sweetness level, fruit weight, and water footprint. Data were analyzed using ANOVA and DMRT. Applying 2.8 mS cm-1 nutrient solution (NS) + 60 mg L-1 GA3 was the best treatment according to fruit diameter, edible part thickness, and fruit sweetness level. Although statistically, it had no significant effect, 2.8 mS cm-1 NS + 60 mg L-1 GA3 increased fruit weight by 18.75% and water footprint efficiency by 15.48% compared to control. Keywords: blue water footprint; evapotranspiration; gibberellins; nutrient solution
Pengaruh 2,4-D dan BAP terhadap Multiplikasi Tanaman Vanili (Vanilla planifolia Andrews): Pengaruh 2,4-D dan BAP terhadap Multiplikasi Tanaman Vanili (Vanilla planifolia Andrews) Restanto, Didik Pudji; Nadiya, Nisma Riyadh; Dewanti, Parawita; Khozin, Mohammad Nur; Prayoga, Mohammad Candra
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 9 No 2 (2025): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v9i2.912

Abstract

Vanilla is a plant that has many benefits, high selling value and potential to be developed. Conventional propagation of vanilla such as through cuttings has not been effective, so a more effective method of propagation is needed, one of which is propagation through tissue culture. In-vitro propagation of vanilla can be through shoot multiplication to produce fast plantlets. One of the successes of shoot multiplication is influenced by the hormone given. This study aims to determine the effect of giving a combination of 2,4-D and BAP on the multiplication of vanilla shoots. The study design used CRD with a combination factor of 2,4-D (concentration 0.5 mg/L, 1.0 mg/L, and 1.5 mg/L) and BAP (concentration 0.25 mg/L, 0.5 mg/L, and 0.75 mg/L). The explants used were vanilla stem segments. Based on the research results, the combined treatment with concentrations of 2,4-D 1 mg/L and BAP 0.5 mg/L had a very significant effect on the multiplication of vanilla shoots with the fastest emergence of shoots with an average of 6.6 wap, the highest number of shoots was 7 shoots, and the survival percentage was 100%.
Respon kultur antera tembakau terhadap media bernutrisi dan AgNPs secara in vitro Khozin, Mohammad Nur; Ahnaf, Yusuf Dary; Dewanti, Parawita; Restanto, Didik Pudji; Iryono
Jurnal AGRO Vol. 12 No. 2 (2025)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/j.agro.49119

Abstract

Tanaman tembakau (Nicotiana tabacum L.) merupakan tanaman musiman bernilai ekonomi tinggi, namun produksinya di Indonesia terus mengalami penurunan dalam beberapa tahun terakhir. Penggunaan varietas hibrida dapat menjadi solusi karena memiliki produktivitas dan ketahanan penyakit yang lebih baik, tetapi pembentukannya secara konvensional memerlukan waktu lama. Teknik kultur antera dapat digunakan untuk mempercepat pembentukan galur murni karena menghasilkan tanaman homozigot hanya dalam satu generasi. Penelitian ini bertujuan untuk menganalisis interaksi antara jenis media kultur (MS dan Chu’s N6) dengan penambahan beberapa konsentrasi Nanopartikel silver (AgNPs) yaitu 0,0; 2,5; 5,0; 7,5; dan 10,0 mg L⁻¹ terhadap pertumbuhan antera tembakau secara in vitro. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 10 perlakuan dan 3 ulangan, kemudian data dianalisis menggunakan ANOVA dan diuji lanjut DMRT dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa media MS merupakan media terbaik dalam mempercepat dan memperbanyak induksi swelling, kalus, tunas, dan akar, sedangkan konsentrasi AgNPs terbaik diperoleh pada 2,5 mg L⁻¹ yang mampu meningkatkan respons pertumbuhan serta sedikit mengurangi tingkat kontaminasi media. Hasil penelitian ini menunjukkan bahwa kombinasi media MS dan AgNPs dosis rendah mampu meningkatkan efisiensi regenerasi antera tembakau dan berpotensi digunakan sebagai protokol standar dalam produksi galur murni secara cepat, efisien, dan steril di laboratorium kultur jaringan. ABSTRACT Tobacco (Nicotiana tabacum L.) is a seasonal crop with high economic value, but its production in Indonesia has continued to decline in recent years. The use of hybrid varieties can be a solution because they have better productivity and disease resistance, but their conventional formation requires a long time. Anther culture techniques can be used to accelerate the formation of pure lines because they produce homozygous plants in just one generation. This study aims to determine the interaction between the types of culture media (MS and Chu's N6) with the addition of several concentrations of silver nanoparticles (AgNPs), namely 0, 2.5, 5, 7.5, and 10 mg L⁻¹ on the growth of tobacco anthers in vitro. The study used a Completely Randomized Design (CRD) with 10 treatments and 3 replications, then the data were analyzed using ANOVA and further tested with DMRT at the 95% level. The results showed that MS medium was the best medium in accelerating and increasing the induction of swelling, callus, shoots, and roots, while the best concentration of AgNPs was obtained at 2.5 mg L⁻¹ which was able to increase the growth response and slightly reduce the level of media contamination. The findings of this study indicate that the combination of MS medium and low-dose AgNPs can increase the efficiency of tobacco anther regeneration and has the potential to be used as a standard protocol in the production of pure lines quickly, efficiently, and sterilely in tissue culture laboratories.
Combination Effect of Culture Media and Silver Nanoparticle on the Effectiveness of Tobacco Anther Regeneration Nur Khozin, Mohammad; Ahnaf, Yusuf Dary; Dewanti, Parawita; Restanto, Didik Pudji; Iryono
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.681-693

Abstract

Anther culture is an effective biotechnological approach to accelerate the production of homozygous lines in tobacco breeding. This study evaluated the interaction between culture media (Murashige and Skoog/MS and Chu’s N6) and silver nanoparticle (AgNP) concentrations on in vitro anther regeneration of tobacco (Nicotiana tabacum L.). The experiment was arranged in a completely randomized design with ten treatment combinations consisting of two basal media and five AgNP concentrations (0, 2.5, 5, 7.5, and 10 ppm), each replicated three times. Quantitative parameters included callus formation, shoot regeneration, rooting response, regeneration percentage, and contamination rate. MS medium supplemented with 2.5 ppm AgNPs (K2) produced the optimal response, showing 100% callus formation and the highest shoot regeneration percentage (89%). Root formation was observed only in treatments K2 and K4. In contrast, higher AgNP concentrations (≥7.5 ppm), particularly in Chu’s N6 medium, significantly reduced regeneration percentages (ANOVA, p<0.05). The promotive effect of low AgNP concentration is associated with suppression of ethylene activity, antimicrobial action, and regulation of reactive oxygen species (ROS), which collectively enhance cell division and organogenesis. These findings indicate that MS medium supplemented with 2.5 ppm AgNPs is optimal for tobacco anther regeneration and suitable for doubled-haploid production.
Pendampingan masyarakat untuk pembentukan TABOGA Farm guna peningkatan kesejahteraan petani di desa Klangon kabupaten Madiun Alfarisy, Fariz Kustiawan; Restanto, Didik Pudji; Widuri, Laily Ilman; Witono, Yuli; Fajrin, Fifteen Aprila; Paramu, Hadi
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 8, No 2 (2024): June
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v8i2.22740

Abstract

AbstrakDesa Klangon saat ini menjadi desa rujukan tanaman porang Indonesia. Tidak hanya itu Desa Klangon memiliki wisata klangon yang terletak di tengah hutan klangon. Tujuan dari pengabdian masyarakat adalah memberikan pemberdayaan dan pendampingan masyarakat mengenai implementasi Agro Smart Village sebagai solusi pengelolaan wisata dan TABOGA dalam upaya peningkatan kemandirian dan derajat kesehatan masyarakat. Tahapan pelaksanaan kegiatan meliputi koordinasi dengan perangkat desa,  FGD (Focus Group Discussion) dan praktek di beberapa kelompok masyarakat, pendampingan, pembentukan TABOGA farm, dan pendampingan, Pendampingan dalam perencanaan pengembangan usaha hilirisasi produk TABOGA. Kegiatan pengabdian desa Asal melibatkan beberapa perangkat desa, tokoh masyarakat, dan petani yang menjadi sasaran. Hasil yang didapatkan dari kegiatan pengabdian ini diantaranya adalah komoditas TABOGA di desa klangon memiliki potensi yang besar untuk dikembangkan sebagai komoditas unggulan kedua setelah porang. Setidaknya dari hasil FGD tercatat sebanyak 18 jenis tanaman obat tradisional yang banyak tumbuh berdampingan dengan tanaman porang di hutan Desa Klangon. Namun, potensi ini belum banyak dimanfaatkan secara maksimal. Permasalahan utama yang menjadi kendala adalah pemasaran produk TABOGA. Berdasarkan permasalahan tersebut, kontribusi yang diberikan oleh Tim Pengabdi pada kegiatan ini yakni  pendampingan implementasi Agro Smart Village melalui pemetaan komoditas TABOGA untuk meningkatkan minat masyarakat dalam mengembangkan komoditas TABOGA untuk kesejahteraan masyarakat Desa Klangon. Kata kunci: pemasaran; porang; tanaman obat tradisional; wisata. AbstractKlangon Village is currently a reference village for Indonesian porang plants. Klangon Village has a Klangon tourist area which is in the middle of the Klangon forest. The purpose of community service was to provide community empowerment and assistance regarding the implementation of Agro Smart Village as a tourism management solution and TABOGA to increase self-sufficiency and community health status. The method of implementing the assisted village service activities was carried out using two methods, namely FGD (Focus Group Discussion) and practiced in several community groups. The production technology practice method aimed to aid the community regarding the formation of TABOGA Farm. Origin village service activities involved several village officials, community leaders, and targeted farmers. Based on the results of the community service activities, the Klangon people required assistance from universities to make mapping of TABOGA commodities and their marketing. Farmers need institutional support such as BUMDES to become a business unit that accommodates community taboga plant products so that it is hoped that they can increase people's income.Keywords: tourism; traditional medicinal plant; marketing.
Peningkatan Ketahanan Tanaman Kedelai (Glycine max (L.) Merril) Terhadap Penyakit Antraknosa Melalui Induksi Mutasi Sodium Azide Fathoni, Rizal Dwi; Hartatik, Sri; Restanto, Didik Pudji
Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Vol. 23 No. 2 (2025): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v23i2.4774

Abstract

Kedelai (Glycine max (L.) Merril) merupakan tanaman dengan kandungan nutrisi yang tinggi, tapi produktivitas kedelai cukup rendah, hal ini disebabkan oleh serangan penyakit Antraknosa. Penelitian ini bertujuan untuk meningkatkan ketahanan tanaman melalui induksi mutasi sodium azide. Percobaan dilakukan pada Februari 2021 - Juli 2022 di Greenhouse Jl. Prajurit Syakur, Kecamatan Srono, Kabupaten Banyuwangi. Percobaan ini dilakukan melalui beberapa tahapan yaitu; Perendaman benih kedelai varietas Wilis dan Grobogan dengan larutan sodium azide pada konsentrasi 0mM dan 0,663mM, Penanaman benih kedelai hasil induksi mutasi, Inokulasi Antraknosa, dan Pengamatan ketahanan tanaman. Data yang didapatkan dianalisis dengan menggunakan data deskriptif. Induksi mutasi sodium azide pada 0,663mM dapat meningkatkan ketahanan tanaman terhadap penyakit Antraknosa. Ketahanan tanaman dilihat dari persentase tingkat keparahan penyakit. Terdapat 2 kriteria tingkat keparahan penyakit yaitu Moderate (Wilis dan Grobogan 0mM, Grobogan 0,663mM) dan Resisten (Wilis 0,663mM).
Ethnobotany of Amorphophallus muelleri var. Madiun I: Culture and Nutraceutical Study in Klangon Village of Madiun Regency, Indonesia Putri, Widya Kristiyanti; Restanto, Didik Pudji; Qonitah, Nisrina
Jurnal Ilmu Lingkungan Vol 23, No 6 (2025): November 2025
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.23.6.%p

Abstract

Klangon Village, Saradan District in Madiun Regency is one of the largest porang-producing villages in Indonesia with export quality results, making it one of the Porang Center villages. Porang cultivation is believed to have been carried out from generation to generation in an agroforestry system. This study aims to reveal more about the role of porang for the people of Klangon Village, both in terms of culture and its relationship to the phytochemical content of porang. The study was conducted through observation, ethnobotanical data collection, phytochemical data collection as nutraceuticals, and data analysis. Phytochemical data was collected using spectrophotometry while spatial data was collected using the GPS Essentials application. Ethnobotanical analysis was carried out using the Cultural Significance Index (CSI). Nutraceutical analysis by testing the content of vitamins using the spectrophotometric method. It was concluded that Ethnobotanical studies of porang plants show the highest CSI values in porang plants and nutraceutical studies of porang show the content of antioxidants (vitamin E), vitamins B1, and B6.
Co-Authors . Usmadi Adinda, Intan Dwi Agnes Natalia Wijaya Ahnaf, Yusuf Dary Aji, Seto Purnomo Alfarisy, Fariz Kustiawan Anang Syamsunihar Antono, Moh Nuri Aprila Iga Mufidah Arsyika, Izna Arya Wiranegara Azizah, Kunti Anis Azizah, Maria Bambang Kuswandi Bambang Sugiharto Bintoro, Jatmiko Hardi Budha, Al Sura Tri Budi Kristanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto, Budi Calista, Zalza Candra Prayoga, Mohammad Darah Pertami, Rindha Rentina Dinata, Gallyndra Fatkhu Dodi Wijaya Dwi Erwin Kusbianto Dwika Nano Hariyanto Etik Wukir Tini Fanata, Wahyu Indra Duwi Farlisa, Veronenci Yuliarbi Fathoni, Rizal Dwi Fenny Irawati Fifteen Aprila Fajrin Firdauzi, Sandy Al Firdha Narulita Alfian Hadi Paramu Haliza, Nurhayadatul Handayani, Etty Hanif Fatur Rohman Hanifah, Fairuz Luthfi HARDIAN SUSILO ADDY Harsanti, Restiani Sih Indraloka, Aldy Bahaduri Indri Fariroh Iryono Kacung Hariyono Ketut Anom Wijaya Khozim, Mohammad Nur Khozin, Mohammad Nur Kriswanto, Budi Kurniasari, Leli Kushariyadi Kushariyadi Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Luluk Noviana Lutfi Pramukyana Mochammad Wildan Jadmiko Mohammad Nur Khozim Mona, Muhammad Dima Say Muhammad Dima Say Mona Muhammad Nur, Kholid Rosyidi Munandar, Denna Eriani Murtaqib Murtaqib Nadiya, Nisma Riyadh Nafiah, Nur Lailin Nafisah Iqmatullah Natasha Florenika Nur Lailin Nafiah Nurfika Asmaningrum Nurhasna, Rifqi Pamungkas, Wahyu Eka Parawita Dewanti Parawita Dewanti Poppy Hartatie Hardjo Popy Hartatie Hardjo Prayoga, Mohammad Candra Putri, Widya Kristyanti Qonitah, Nisrina Rahmawati Rahmawati Raisah Bani Ratnasari, Tri Refa Firgiyanto Refa Firgiyanto Resti, Ricke Windi Rizqi Maulana Rohman, Fadil Rusdiana, Riza Yuli Saputro Gumelar, Bachtiar Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Simamora, Endah Cahyani Slameto . Slameto Slameto Slameto Slameto Slameto Slameto Sri Hartatik Sukri, M Zayin Suliswanto, Eko Nur Sundahri, Sundahri Susanto, Arnis Budi Thohirin, Ahmad Nazmi Thongiratama, Michael Anthony Tri Agus Siswoyo TRI HANDOYO Tri Handoyo Tri Ratnasari Tri Rini Kusparwanti, Tri Rini Tri Widyastuti Ubaidillah, Mohammad Ummi Solikhah, Ummi Vega Kartika Sari Veronenci Yuliarbi Farlisa Wahyu Indra Duwi Fanata Widya Kristiyanti Putri Widya Kristiyanti Putri Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Witono Zahro, Fauziatuz