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Adi Rahmat
Prodi IPA
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Journal : Journal of BioSustainability

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Response of Pinus merkusii Jung. & Devr. Megagametophyte Explants Cultured on DCR Medium with Different 2,4-D and Kinetin Combination Nabila, Afini Zulafa; Rahmat, Adi; Kusdianti, Kusdianti; Yulianty, Nurfitri
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Publisher : Universitas Pendidikan Indonesia and Konsorsium Biologi Indonesia

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Abstract

Pinus merkusii Jung. Devr. is a multipurpose tree species that is widely cultivated for timber, resin production, and land conservation. Nearly all parts of the tree are utilized, making it highly valuable. However, the high demand for its products is not met by its low natural regenerative capacity, which poses challenges for sustainable management. To address this, somatic embryogenesis, an in vitro propagation technique, has been explored as an alternative method for multiplying conifer species like Pinus merkusii. This study aims to assess the in vitro response of megagametophyte explants cultured on DCR medium with various combinations of 2,4-D and kinetin. The concentrations of 2,4-D tested were 0 µM, 7 µM, 9 µM, and 11 µM, while kinetin was tested at 0 µM, 2 µM, 3 µM, and 4 µM. The explants used were megagametophytes from female strobili measuring 5-7 cm, containing young zygotic embryos in the preembryo stage. After two months of cultivation in the dark, the explants exhibited responses such as enlargement, callus formation, and germination. The highest response rates for explant enlargement, callus formation, and germination were observed in the combinations of DK00 and DK04 (33.33%), DK70 and DK114 (33.33%), and DK72 and DK114 (44.44%). No somatic embryo formation was observed, possibly due to the age of the explants.
Co-Authors Abdillah , Rasyid Adolf Bastian afred suci, afred Agusta, Muhammad Adrian Ahmad, Nur Jahan Aldri Frinaldi Amri, Muhammad Fahriza Anna Fitri Hindriana Aprilia, Yasinta Arif Sura, Sunarto Arif, Sunarto arizal N, arizal Ashidiq, Rizki Maulana Aznuriyandi, Aznuriyandi Bachtiar, Ahmad Arif Bariroh, Ghurrotul Brata, Wasis Wuyung Wisnu Cita Tresnawati, Cita Damayanti, Anggia Fitri Dewi Susanti Dharma Satria, Hengky Didik Priyandoko Dr. Riandi Dr. Saefudin Dwika Listihana, Wita Dwika Lodia Putri Eka Cahya Prima Euis Erlin Fadillah*, Rofi Wafa Fatkhurahman Fiandini, Meli Fitriani, Salma Gunawan, Eryk Gunawan, Syahreza Andika Hadibroto, Tiara Sri Annisa Hadiyati Hadiyati, Hadiyati Hamby, Suhardiman Handayanie, Yunni Hanif, Norfazrin Mohd Hartini, Venty Sugih Heri, Helwen Indrawan, Fery Jannah, Novi Dewi Khadikatul jeki Jeni Wardi Jumarno Juniarti, Mia Angrianan Karimi, Amir Karwatisari, Rina Kasmadi Kasmadi Kurniawan, Luthfiyah Azzahra Kusdianti Kusdianti Kusnadi Kusnadi, Erik Witular Lala Septem Riza Lepir, Yenika Rambu Luthfia, Ihda Siti Mr Amprasto Mrs Yanti Hamdiyati Mubiar Agustin Muhammad Rasyid Abdillah Munthe, Richa Afriana Muslihah, Fitriyyatul Muzzamir Nabila, Afini Zulafa Nanang Winarno Nasution, Imran Al Ucok Nono Sutarno Nur Rahman, Nur Nurhayati Nurhayati Nuryani Rustaman Oemar, Fahmi Purwati K. Suprapto Putra, Berry Arta Putri, Iffa Ichwani Putri, Meirin Dwiningtyas Qisthi, Naufalia Raffila Irawan, Raja Rahmi, Annisa Salsyabila Riandi Riandi Riandi Riki Zaputra Rinaldy, Febri Rini Solihat Ririn Handayani Risma Munandar, Rifki Rizal, Ayong Rizqa Anita Rizqi, Liza Rohimah, Tina Rizqiyati Rosinta Septiana, Rosinta Saefudin Saefudin Safarini, Melly Salsabila, Azura Sapriyanda Saputra, Winata Tegar Saputri, Nova Vivi Clara Sepita Ferazona seswandi, agus Sihombing, Rizky Agassy Siti Sriyati Sri Bulan Nasution Sri Redjeki Sunandar, Aris Suwandi, Tri Syaakir, Ibnu Syahirah, St Syahirah Syahirah, St. Syahsudarmi, Siti Syamsiar, Syamsiar Sylva Sagita Tamba, Yusnita Renata Taufik Rahman Tengku Idris, Tengku Topik Hidayat Tri Nanda, Satria Umar, Mohammad Farhan Venty, Venty Sugih Hartini Verawati, Yenni Wahidin, Naufal Rabah Waris Wuyung WB Widi Purwianingsih Winarno*, Nanang YAYAN SANJAYA Yogi Yunefri, Yogi Yuliana, Dewi Rafna Yulianty, Nurfitri Yusnidar Yusnidar Yusuf Ibrahim Zakiya, Umi Sayidatus Zulhendra, Zulhendra