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The effects of varying in incubation time and temperature of methyl salicylate as a clearing agent on the quality of breast tissue slides Fathiasari, Fariha; Wiryanti, Wiwin; Durachim, Adang; Rahmat, Mamat
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.4812

Abstract

Non-polar clearing agents have the ability to dissolve breast adipose tissue. While fat dissolution occurs during the clearing process, careful monitoring of adipose cell membranes mis crucial due to their impact on the behavior of breast cancer cells and disease progression. Xylol is a widely employed clearing agent. However, its toxicity and prolonged clearing time necessitate the exploration of alternatives. In this context, methyl salicylate emerges as a viable substitute. It is non-polar nature facilitates efficient fat dissolution, leading to quicker tissues clarification. This study adopted a quasi-experimental method, utilizing 24 pieces of breast tissue as the sample. These tissue sample were divided into six groups according to the treatment regimen. The treatment groups involved breast tissue clearing using xylol for 2x60 minutes at room temperature, methyl salicylate for 2x30 minutes at room temperature, and methyl salicylate at 60°C for 20 minutes, 30 minutes, 2x20 minutes, and 2x30 minutes. The quality of breast tissue slides was assessed by analyzing the color contrast between the nucleus and cytoplasm using ImageJ software, along with the clarity of adipose cell cell membrane using microscopy. The results indicated that prolonged exposure at high temperature resulted in poor quality breast tissue slides. Conversely, tissue clearing with methyl salicylate for 2x30 minutes at room temperature exhibited excellent contrast between the nucleus and cytoplasm, as well as clear adipose cell membranes. Further study is warranted to explore the applicability of methyl salicylate as a clearing agent in tissues with lower fat content.
Extraction Of Brazilin From Sappan Wood as a Natural Reagent for Borax Detection by Digital Imaging Analysis Rismiarti, Zuri; Rahmat, Mamat; Nur Indra, Asep Iin; Syaindi, Amanda Afifah
Jurnal Kesehatan Prima Vol 18, No 2 (2024): AUGUST
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkp.v18i2.1440

Abstract

The use of brazilien for borax detection is because the chemical properties of this compound are greatly influenced by pH which results in a change in the color of the brazilien. This compound gives an orange color at a pH range of 6-7 and yellow at an acidic pH, while at a pH range of 7-10 it gives a red color and at a pH >10 it gives a purplish red color. This research uses Brazilian extract from sappan wood for borax detection. By utilizing this compound extract, the use of chemical reagents for the detection of borax in food samples can be minimized. Apart from that, it can increase the added value of these crop commodities and can take advantage of the abundant natural resources in Indonesia. This research aims to develop a method that is accurate, simple and cheap and does not use special instruments to diagnose borax based on digital imaging, which includes extraction of brazilien from sappan wood using a maceration method using 96% ethanol solvent and water with a UV-Vis spectrophotometer, ratio optimization brazilin extract with boric acid, optimization of measurement time. The results of the research show that the solvent that provides optimum absorbance for brazilin extraction is water or distilled water, with a ratio of brazilin extract and boric acid of 1:1 and the measurement time is 5 minutes.
Peran Organisasi Kemasyarakatan Islam dalam Pengembangan Mutu Pendidikan Islam di Indonesia Rahmat, Mamat
Tsamratul Fikri | Jurnal Studi Islam Vol 17 No 1 (2023): Tsamratul Fikri | Jurnal Studi Islam
Publisher : Asosiasi Mahasiswa Program Pascasarjana | Universitas Islam Darussalam (UID) | Islamic University of Darussalam | Ciamis | Jawa Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36667/tf.v17i1.1351

Abstract

This study aims to explore the role of Islamic community organizations in developing the quality of Islamic education in Indonesia. This study uses a literature review method, in which the researcher reviews books, journal articles, research reports, and proceedings of relevant scientific forums. The results of the study show that Islamic community organizations in Indonesia have a great influence on national development. In the context of the development of education in Indonesia, Islamic community organizations have played a significant role in advancing education and developing quality education in the country. The role of Islamic community organizations in advancing Islamic education and developing the quality of education has certainly been felt by the community. Community organizations such as Muhammadiyah, Nahdlatul Ulama (NU), Persatuan Islam/Islamic Association (Persis), Persatuan Umat Islam/Islamic Community Association (PUI) and others, are believed to have made a real contribution in advancing educational institutions in Indonesia. These Islamic community organizations together with the community have proven to have played a significant role in developing the quality of education as well as in equitable distribution of education.
ANALISIS KORELASI PERILAKU BELAJAR DENGAN HASIL BELAJAR PESERTA DIDIK KELAS V SD DI KOTA SERANG Subagio, Rohmad; Rahmat, Mamat; Ikrom, Fadhli Dzil
Journal of Professional Elementary Education Vol. 3 No. 1 (2024): Journal of Professional Elementary Education
Publisher : LPPM Universitas Bina Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46306/jpee.v3i1.62

Abstract

Student behavior greatly influences the achievement of learning outcomes because learning is adding and acquiring a certain amount of knowledge. Learning outcomes are generally defined as the relationship between students and the systems around them during the schooling process, which leads to changes in behavior in the cognitive, affective and psychomotor fields, which are represented by symbols or numbers. The research conducted focused analysis on the relationship or correlation between learning behavior and learning outcomes of elementary school students in Serang City. The aim of this research is to analyze the relationship or correlation between learning behavior and learning outcomes of elementary school students in Serang City. Data collection techniques were obtained using learning behavior questionnaires and observing student learning outcomes. Sampling was carried out using simple random sampling techniques in three elementary schools in Serang City, totaling 90 students. The research results show that the average value of student learning outcomes is 83.60. Prerequisite testing, the normality test is carried out with a normal probability plot test which is quite effective in detecting whether a study has a normal distribution or not. Based on this test, the data is normally distributed because the points on the diagram always follow and approach the diagonal. The significance value of the learning behavior variable with the learning outcome variable is 0.000 where 0.000 < 0.05, so there is a correlation between the learning behavior variable and the student's learning outcomes. The test results show that there is a significant relationship or correlation between learning behavior variables and student learning outcomes
PENGARUH VARIASI VOLUME SAMPEL DARAH DAN DIAMETER SEDIAAN DARAH TEBAL PADA PEMERIKSAAN Plasmodium falciparum Kawaitouw, Leberina; Mulia, Yuliansyah Sundara; Sulaeman, Sulaeman; Rahmat, Mamat
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 2 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i2.2540

Abstract

Pemeriksaan Plasmodium secara mikroskopik merupakan gold standart dalam diagnosis infeksi. Sampel darah yang akan diperiksa dibuat sediaan darah tebal dan tipis. Sediaan darah tebal menggunakan sampel darah lebih banyak sehingga pada kasus malaria ringan, parasit mudah ditemukan. Tujuan penelitian untuk mengetahui perbandingan antara volume sampel darah 8µl, 10µl dan 12µl pada diameter 1cm dan diameter 1,5cm terhadap jumlah parasit P.falciparum. Penelitian ini menggunakan metode quasi eksperimen. Bahan pemeriksaan adalah darah EDTA 3mL yang diperoleh dari pasien terkonfirmasi positif P.falciparum, dilakukan pembuatan sediaan darah tebal dengan volume darah 8µl, 10µl dan 12µl pada diameter 1cm dan 1,5cm yang di multipikasi sebanyak 30 sediaan. Selanjutnya sediaan diwarnai dengan Giemsa 3% dan diperiksa secara mikroskopik menggunakan metode perhitungan kuantitatif. Data dianalisis menggunakan uji statistik Anova dengan derajat kepercayaan 95% didapatkan nilai signifikan pada diameter 1cm nilai 0.106>0.005 maka hipotesa Ha diterima. Pada sediaan darah diameter 1,5cm nilai 0.000<0.005, maka hipotesa Ho diterima. Kesimpulan adalah tidak terdapat perbedaan hasil jumlah P.falciparum pada volume sampel darah 8µl, 10µl dan 12µl pada diameter 1cm dan terdapat perbedaan jumlah P.falciparum pada volume 8µl, 10µl dan 12µl pada diameter 1,5cm.
EFEKTIVITAS PENGGUNAAN MEDIA SOSIAL TIKTOK SEBAGAI MEDIA EDUKASI PENCEGAHAN KEK DI SMAN 6 CIMAHI Berlian, Selpia; Mahmudah, Umi; Suparman, Suparman; Rahmat, Mamat
Jurnal Gizi dan Dietetik Vol. 3 No. 2 (2024): Jurnal Gizi dan Dietetik
Publisher : Jurusan Gizi Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jgd.v3i2.2776

Abstract

Chronic Energy Deficiency (CED) is prevalent among Indonesian young women, increasing the risk of stunting. Early education, including the use of TikTok, is recommended to prevent CED due to its popularity and accessibility among teenagers. This research aims to determine the effect of nutritional education regarding the prevention of CED through the social media TikTok on knowledge, attitudes and protein energy intake in young women at SMAN 6 Cimahi. The design of this research is a quasi experiment with a pre test - post test with control group design. Simple random sampling was used with a sample size of 52 people, divided into treatment and control groups for education via TikTok and PowerPoint. Data analysis employed various statistical tests like Shapiro Wilk, Wilcoxon, and T-Tests. The research results show that there is an influence of TikTok social media on knowledge, attitudes, energy and protein intake in young women at SMAN 6 Cimahi. In terms of increasing knowledge and attitudes, TikTok social media is more effective as an educational medium than PowerPoint media (p=0.000), but is not effective for energy intake (p=0.103), and is not effective for protein intake (p=0.169). Further research to determine the effectiveness of TikTok social media on energy and protein intake will need to be accompanied by mentoring methods.
PERAN PAGUYUBAN ORANG TUA SISWA DALAM MENINGKATKAN MANAJEMEN BERBASIS KOLABORASI DI MADRASAH IBTIDAIYAH Nurhakim, Haditsa Qurani; Rohili, Ilyas; Ruswandi, Irwan; Rahmat, Mamat; Erihadiana, Muhammad
Islamic Management: Jurnal Manajemen Pendidikan Islam Vol. 7 No. 001 (2024): Islamic Management: Jurnal Manajemen Pendidikan Islam Special Issue
Publisher : Sekolah Tinggi Agama Islam Al Hidayah Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30868/im.v7i001.7543

Abstract

Penelitian ini bertujuan untuk mengkaji peran paguyuban wali siswa dalam meningkatkan manajemen kolaboratif di MIN 13 Ciamis dengan pendekatan kualitatif dan metode studi kasus. Data dikumpulkan melalui wawancara mendalam, observasi partisipatif, dan dokumentasi dari kegiatan paguyuban. Peneliti ini bertujuan untuk mengidentifikasi peran paguyuban dalam meningkatkan manajemen berbasis kolaborasi di madrasah ibtidaiyah. Hasil penelitian menunjukkan bahwa paguyuban berfungsi sebagai jembatan komunikasi yang efektif antara sekolah dan orang tua, yang meningkatkan keterlibatan orang tua dalam berbagai kegiatan sekolah. Keterlibatan aktif orang tua melalui paguyuban tidak hanya memperkuat hubungan antara sekolah dan wali siswa, tetapi juga berkontribusi pada keberhasilan program pendidikan dan pengembangan karakter siswa. Meskipun ada tantangan seperti keterbatasan waktu bagi orang tua dan perbedaan pandangan mengenai kebijakan pendidikan, muncul dampak positif dari kolaborasi ini terhadap kualitas pendidikan di MIN 13 Ciamis. Temuan ini menegaskan pentingnya paguyuban wali siswa sebagai komponen vital dalam manajemen kolaboratif, yang dapat mendorong keberhasilan pendidikan di tingkat dasar dan diharapkan memberikan wawasan bagi pengembangan model kolaborasi yang lebih baik di sekolah sekolah lainnya.
Effect of Variation Natrium Sitrat Concentration and Centrifugation Time on Prothrombin Time (PT) Value Najiyyah, Amalia Nur; Marliana, Nina; Hayati, Eem; Rahmat, Mamat
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.694

Abstract

Background & Objective: Prothrombin Time (PT) test is one of the hemostasis tests used to determine whether there is a deficiency of extrinsic clotting factors and co-pathway clotting factors, namely factors I, II, V, VII, and X. The pre-analytical stage that must be considered in the hemostasis examination is the concentration of anticoagulant used and the centrifugation process. This study was conducted to determine the effect of variations in sodium citrate anticoagulant concentration and centrifugation time on Prothrombin Time (PT) values.  Method: The anticoagulants used in this study were 3.2% and 3.8% sodium citrate. Centrifugation is the process of separating cells from plasma. The effect of improper centrifugation is to increase the number of platelets, so that platelet factors contained in platelets will accelerate the formation of fibrin. The research conducted is a pseudo-experimental study by giving treatment to blood specimens that will be added with anticoagulant Sodium Citrate concentrations of 3.2% and 3.8% with a blood ratio of 9:1, then centrifuged at 3000 rpm and given a variation of time for 15 minutes, 10 minutes, and 5 minutes. The results were tested using Saphiro Wilk and Friedman Test. Result : Based on the research that has been done, the Asymp. Sig. value obtained in the Friedman Test is >0.05. This indicates that the treatment that has been given to the sample has no effect on the value of Prothrombin Time. Conclusion: It can be concluded that there is no significant difference between the variation of sodium citrate concentrations of 3.2% and 3.8%, as well as in the variation of 3000 rpm centrifugation time of 15 minutes, 10 minutes, and 5 minutes on the value of Prothrombin Time (PT).
Formulasi Jagurah Tepung Ubi Jalar Kuning, Tepung Jagung Kuning, dan Tepung Beras Merah sebagai Snack bagi Penderita Diabetes Melitus Andrafikar, Andrafikar; Mahmudah, Ummi; Rahmat, Mamat; Gumilar, Mulus
Jurnal Sehat Mandiri Vol 19 No 2 (2024): Jurnal Sehat Mandiri, Volume 19 Nomor 2 Desember 2024
Publisher : Poltekkes Kemenkes Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33761/jsm.v19i2.1741

Abstract

The prevalence of diabetes mellitus (DM) in Indonesia is predicted to increase from 10.7 million in 2019 to 13.7 million by 2030. If not properly managed, DM can lead to various complications, making proper management essential. One such approach is to provide foods with a low glycemic index and high soluble fiber content. This study aims to examine the organoleptic properties and fiber content of modified baked sagun (Jagurah). The research design was an experiment using a completely randomized design (CRD) with three treatments, conducted in the Food Technology Laboratory, Department of Nutrition, Padang. Organoleptic testing involved 25 trained panelists using a 7-point scale, while proximate analysis was performed at the BSPJI Laboratory in Padang. Data analysis was conducted using the Mann-Whitney test for normal data and Kruskal-Wallis test for non-normal data. The results showed no significant differences in color, texture, aroma, and overall acceptability (p > 0.05), but there was a significant difference in taste (p = 0.00). The best formula was F3. The best Jagurah contains 327 kcal, 12.07 g protein, 11.5 g fat, 43.8 g carbohydrates, and 6.75 g fiber per 100 g. The price for a 60 g serving is Rp 4,000.
PENGARUH KEPEMIMPINAN KEPALA SEKOLAH DAN PERAN AKTIF DUNIA INDUSTRI TERHADAP MUTU SEKOLAH MENENGAH KEJURUAN Wiratmoko, Aris; Subagio, Rohmad; Rahmat, Mamat
JIPIS Vol. 33 No. 2 (2024): Oktober 2024
Publisher : FKIP, UNIVERSITAS ISLAM SYEKH-YUSUF TANGERANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33592/jipis.v33i2.4795

Abstract

Penelitian ini bertujuan untuk mengetahui: (1) pengaruh langsung kepemimpinan kepala sekolah terhadap mutu sekolah, (2) pengaruh langsung peran aktif Dunia Industri terhadap mutu sekolah dan (3) pengaruh kepemimpinan kepala sekolah terhadap peran aktif Dunia Industri. Penelitian ini dilakukan di Sekolah Menengah Kejuruan di kota Serang. Penelitian ini menggunakan metode kuantitatif dengan metode survey dan analisis jalur diterapkan dalam hipotesis dan 59 responden sampel dipilih dengan menggunakan random sampling. Hasil penelitian menunjukkan bahwa: (1) kepemimpinan kepala sekolah berpengaruh langsung positif terhadap mutu sekolah, (2) peran dunia industri berpengaruh langsung positif terhadap mutu sekolah, dan (3) kepemimpinan kepala sekolah berpengaruh langsung positif terhadap peran dunia industri.