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Nur Rohman
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Isolation, Identification, and Evaluation of Antimicrobial of the LAB from Bekasam: The Traditional Fermented Fish in Indonesia Rohman, Nur; Alim, Muhammad Bachrun
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.897-903

Abstract

Lactic acid bacteria (LAB) are frequently utilized in fermented foods and can incresed shelf life for the products through their secondary metabolites includes carbon dioxide, hydrogen peroxide, lactic acid and bacteriocins. Beside increasing the shelf life, LAB also affect taste, smelt, and texture. Thus, this study aimed to isolate, identify and evaluate antibacterial LAB strains from Indonesian traditional fermented fish (Bekasam). Gram staining, the catalase assay, and motility assays were used to initially characterize the presumed isolates phenotypically following primary isolation on De Man, Rogosa, and Sharper (MRS) agar. 14 isloates were determined to be presumed LAB by preliminary phenotypic testing. One species that has the highest antibacterial activity is confirmed by a 16S rRNA sequencing study. Lactobacillus plantarum CP-134 was identified from the bekasam. In the biochemical characterization all isolates were catalase-negative and non-motile. According to our research, these LAB strains may have applications in fermented foods due to their probiotic properties, which include antibacterial activity. They might be used as natural substitutes for additives and antibiotics, but more in vivo or in silico research is required to verify their potential and effectiveness. A typical traditional food preservation technique for enhancing shelf life, food safety and nutrition and sensory qualities is fermentation. Bekasam is a traditional fermented food from Indonesia.
Co-Authors A Halim Abdul Hakim Prima Yuniarto Abdul Matin Bin Salman Afreni Hamidah Agustina, Elvinda Bendra Ali Mujahidin Alim, Muhammad Bachrun Amin Kiswantoro Aminuyati Andika, Alek Anisah Anita Dewi Utami Annisa Annisa Ari Indriani Arief Wibowo Asep Syaiful Bahri, Asep Syaiful Ashartono, Rakhmat Asih Asih, Asih Asih Wiranti, Dwiana Ayuningtyas, Dian Cahyo Hasanudin Dar Kasih Day Ramadhani Amir Dian Ratna Puspananda Dwi Erna Novianti Efendi, M Shofriyan Eko Haryanto Faizah, Jihan Billah Fauziah, Zuliani Fhadlilla Fharhah, Syilla Firzatullah, Raden Muhamad Fisa, Triansyah Fresiliasari, Oktavie Hamdi Putra Ahmad Hamidaturrohmah, Hamidaturrohmah Hartono, Agus Budi Hasan, Khairuddin Hayatri, Maria Ana Sila Hendratono, Tonny Herman Herman Hermansyah Hermansyah Heru Ismaya Hidayatusholikah, Ning I, Heru Ihlas, Ihlas Inayatul Inayah Irfana, Tungga Buana Isma, Cut Nelga Istiningsih, Istiningsih Jannah, Roihatul Jehan, Adam Jodion Siburian Joko Setiyono Joko Setyono Jovial Pally Taran Junarti Junarti K, Neneng Rika J. Khotijah, Vina St Lailatunnihayah, Lailatunnihayah Laili, Rizkia Nur Leopoldus Ricky Sasongko Lintang, Ferlanda Maisya, Anis Makiya, Kiki Rizki Manggiasih, Ninda Dwi Matin , Moh. Fuadul Mochammad Imron Awalludin Muhaimin, Muh Muhammad Faisal Muhammad Rinov Cuhanazriansyah Munir, Muhammad Misbahul Murni, Intan Permata mutiah, siti Nahar, Afifatun Nahlidar, Nahlidar Nichla Choirin Attalina, Syailin Nofendri, Yos Novi Irawati Noviati, Fitria Nugroho, Agung Yuliyanto Nur Cahyani, Bela Dwi Parmadi, Mokhamad Puput Suriyah Puspitaningsih, Siska Rhamadani, Ilham Rifky, Rifky Rizky Maulana, Rizky Rizqiyah, Mufidah Rofiqoh, Naili Roni Hidayat Salsabila, Niken Alya Salsabila, Syifa Saparuddin Saparuddin Sari, Desti Yuvita Sari, Eva Dwi Puspita Satria, Teuku Dwirza Sely Ayu Lestari Shimbun, Annisa Fikria Siti Alliyah Solehhudin, Muhamad Sri Sutarni Sugeng Riyanto Sugiarto S Suharman Suharman, Suharman Sujiran sukri Sukri, sukri Sumardi Efendi Sungkar, Amru Supriyanto Supriyanto Susanto, Dwiyono Rudi Susilayati, Muslimah Sutriyani, Wulan Suwardi Suwardi Syaifi, Mat Tentia, Ine Touwe, Yohana S Toyib, Muhamad Tundjung Mahatma Vebrita, Gina Widiatmanta, Jeka Widiyono, Aan Widyakto, Adhi Yaqin, Muhammad Husnul Zahara Zahara Zahra, Mahar Hazzillia Az Zakiatin, Novelia Rizka Zetia Ramadani, Salsabila Zuriah, Fifi