Wibawati, Prima Ayu
Department Of Veterinary Public Health, Faculty Of Veterinary Medicine, Universitas Airlangga

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Detection of Antibodies Brucella abortus in dairy cattle in Puspo District, Pasuruan using Rose Bengal Test and Complement Fixation Test Kusuma, Agung Jati; Safitri, Erma; Praja, Ratih Novita; Tyasningsih, Wiwiek; Yunita, Maya Nurwartanti; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.199-206

Abstract

Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.
Lime Peel Liquid (Citrus aurantifolia, Swingle) Inhibit Escherichia Coli In Vitro Sari, Devi Indah; Wahjuni, Retno Sri; Praja, Ratih Novita; Utomo, Budi; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.63-71

Abstract

The purpose of this study was to determine in vitro inhibition test of lime peel liquid (Citrus aurantifolia, Swingle) against E. coli. The E. coli isolate was cultured on Muller Hinton Agar (MHA) media. Antibacterial test in this study using diffusion method. Negative control (K-) using CMC-Na 0.1%. Positive control (K+) using oxytetracycline antibiotics. The treatments were given lime peel liquid (Citrus aurantifolia, Swingle) with concentrations of 100%, 75%, 50% and 25%, respectively. The study design using Completely Randomized Design (CRD) method, with six treatments and four repetitions. Data were analyzed by Kruskal-Wallis test, Moreover, the Post Hoc Mann-Whitney method. The inhibition zone analysis results were showed that K + had significant differences compared to all treatments K-, 100%, 75%, 50%, and 25%. K- was found to be significantly different from treatments 100%, 75%, 50% and 25%. The results showed that the lime peel liquid has the potential as an antibacterial activity against the growth of E. coli in vitro. Concentration of 25% has shown the presence of inhibitory zones on Mueller Hinton media that have been inoculated with lime peel liquid.
The Risk Factor of Subclinical Mastitis Incident in Dairy Cattle in KPSP Ijen Makmur, Banyuwangi Ningrum, Indah Puspita; Soeharsono, Soeharsono; Wibawati, Prima Ayu; Dhamayanti, Yeni; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.48-53

Abstract

Mastitis is an inflammatory disease in the internal tissue of the mammary gland. Subclinical mastitis does not show any changes in the shape of the mammary gland and to detect it can be examined the number of somatic cells in milk. Subclinical mastitis uses the California Mastitis Test (CMT) method as preliminary research to obtain preliminary data on the incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency. This research is descriptive research. The detection for this research was carried out by taking milk samples in cows per purposive and reacted CMT reagents issued circularly for 10-15 seconds. Observation of results is determined based on changes inconsistency with the existence of gel and the results of interview farmers as supporting data. The results showed that the influencing factors incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency were milk production, age and amount of lactation. In contrast, the biggest factor for reducing the risk of subclinical mastitis is udder health followed by body conditions measured by Body Condition Score.
Service per Conception, Conception Rate, Calving Rate and Non-Return Rate in Beef Cattle in Kalipuro, Banyuwangi Dinul, Ahmad Rajul; Restiadi, Tjuk Imam; Wibawati, Prima Ayu; Ratnani, Hermin; Saputro, Amung Logam; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.54-61

Abstract

This study aimed to determine the number of reproductive efficiency parameters including service per conception, conception rate, calving rate, and non-return rate for broilers in Kalipuro District, Banyuwangi Regency. The secondary data was collected from the Banyuwangi District Agriculture Office. This research is also intended to dissect factors related to the S/C, CR, CvR, and NRR figures with descriptive analysis, including bio reproduction mastery factors, service factors, and feed factors. The primary data was collected from beef cattle breeders in Kalipuro District, Banyuwangi Regency. In addition, there were IB implementation factors whose data were obtained from questionnaires to inseminators in Kalipuro District, Banyuwangi Regency. The number of S/C broilers in Kalipuro Subdistrict, Banyuwangi Regency was good maintenance, in January-March 2020 of 1.71, 1.99, and 1.74, meanwhile, the number of CR were 58%, 50% 57%, CvR for January-March were 44%, 84%, 53% and NRR for January, February and March were 41%, 49%, and 46%, respectively, were below the standard rate.
The Effectiveness of Ethanol Extract of Gamal Leaves (Gliricidia sepium) on Ascaridia galli Mortality in Vitro Sandy, Berliana Dwi Nandita; Suprihati, Endang; Yudhana, Aditya; Hastutiek, Poedji; Wibawati, Prima Ayu; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.82-87

Abstract

This study aimed to determine the anthelmintic activity of the ethanol extract of gamal leaves (Gliricidia sepium) against Ascaridia galli in vitro. There were six treatment groups and each consisted of four replicates. This study used ten A. galli in each treatment for all replications. Observation and recording of death of A. galli was carried out at the 2nd, 4th, 6th, 8th, 10th and 12th hours. Death of A. galli was declared if there was no movement when clamped with anatomical tweezers. The data obtained were analyzed using the ANOVA test and continued with Duncan's test. The results showed that the ethanol extract of gamal leaves had an anthelmintic effect on A. galli worms in vitro. The extract with a concentration of 5% has almost the same anthelmintic properties as piperazine citrate 10 mg/ml. In conclusion, the higher the concentration of the extract, the higher the anthelmintic properties.
Effect of Robusta Coffee Extract on Histopathological in Mice Testes Induced with Monosodium Glutamat Plumeriastuti, Hani; Weliyani, Trima; Hamid, Iwan Sahrial; Hidanah, Sri; Prastiya, Ragil Angga; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.243-248

Abstract

Monosodium glutamate (MSG) is a sodium salt from glutamic acid which is currently very popular to be used as a food flavoring ingredient to stimulate appetite. Exessive consumption of MSG can disrupt the balance of antioxidants and ROS, and cause the negative effects of oxidative stress on testes. Coffee has high chlorogenic acid, function for antioxidants and reduce the negative effect on cell damage in the testes. This study aims to analyze the effect of robusta coffee extract on histological of mice testes induced with MSG. This study was completely randomized design (CRD), using 20 male mice for 5 treatment groups: K+ (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) orally for 42 days. Histophatological scores were analyzed with Saphiro-Wilk Test and Anova. There is no effect of increasing testicular weight. 0,12 mg MSG can cause a decrease in the number of spermatogonia and spermatocytes. 0,2 mg robusta coffee extract can maintain the number of spermatogonia and spermatocytes.
The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi Hutahaean, Ryan David Pandapotan; Meles, Dewa Ketut; Praja, Ratih Novita; Rahmahani, Jola; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.225-233

Abstract

The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.
Total Plate Count of Broiler Meat at Various Market in Bekasi City in March 2021 Firdausyi, Aulia Amatul; Estoepangestie, Agnes Theresia Soelih; Wibawati, Prima Ayu; Hamid, Iwan Sahrial; Solikhah, Tridiganita Intan; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.157-161

Abstract

Food-safety of broiler meat is important because broiler meat is a foodstuff that can be easily contaminated by biological hazard such as bacteria. This study aimed to determine the total plate count of broiler meat at various market in Bekasi City in March 2021. Amount of samples in this study were 40 samples of broiler meat from 10 markets, samples were taken purposively and each sample used 25 grams from Musculus pectoralis of broiler meat. Samples were examined using pour plate method of total plate count. The result showed that 80% (32 of 40 samples) were exceeded the TPC's limit. The highest value of TPC result was 3,7 í— 107 CFU/g from "H” market.
Risk Factors and Incidence of Feline Lower Urinary Tract Disease (FLUTD) in Sahabat Satwa Genteng Animal Clinic Plumeriastuti, Hani; Novitasari, Seline Putri; Arimbi, Arimbi; Yunita, Maya Nurwartanti; Hamid, Iwan Sahrial; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.15-20

Abstract

The purpose of this study was to determine the risk factors and incidence of Feline Lower Urinary Tract Disease (FLUTD) at the Sahabat Satwa Genteng clinic. A total of 36 samples were evaluated according to research procedures with diagnosis, data collection, urine sampling, dipstick and sediment examination methods, and Chi-square data analysis. The results showed that there was a association between gender, history of castration, and diet on the risk of FLUTD. Meanwhile, there was no association between age and the risk of FLUTD. Clinical signs that often occur in FLUTD disease initiate from stranguria, hematuria, dysuria, periuria, and polykisuria. The results of the dipstick examination founded that 86,1% of cats affected by FLUTD had a normal pH and 13,9% had an alkaline pH. The results of the specific gravity (SG) of cats with FLUTD have values below normal while the protein levels found in all cats suffering from FLUTD have values above normal. Stranguria was found in 69,4% of cats with FLUTD. Leukocytes were also found in all the urine of cats suffering from FLUTD. The most common results of sediment examination were struvite, calcium oxalate, and cystine crystals.
Risk Factors for Feline Otitis in Madiun: A Prospective Study Khasana, Uswatun; Meles, Dewa Ketut; Praja, Ratih Novita; Tyasningsih, Wiwiek; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.29-34

Abstract

This study aimed to determine the risk factors in cats associated with the incidence of otitis. A total of 169 cat samples were evaluated by filling out questionnaires and interviewing owners, then an examination was carried out to determine symptoms and treatment so that appropriate action could be taken. Data were analyzed descriptively and tested using Chi-Square analysis. As a result, there were 26 (15,4%) cats diagnosed with otitis. The results of the analysis of significant risk factors include bathing frequency, ear hygiene, and maintenance patterns that can increase the incidence of otitis. The most common clinical symptoms are pruritus, head shaking and accompanied by earwax. The description of the statement about otitis concluded that as many as 70 cat owners, most of them did not understand otitis.
Co-Authors Abdramanov, Abzal Agustono, Bodhi Al arif, Mohammad Anam Amukti, Pandu Tokoh APRIL HARI WARDHANA Arimbi Aziz, Githa Nurma Boedi Setiawan Budi Utomo Cahya, Rizka Wulan Chozin, Aminullah Alhady Chrismonica, Yunia Chusniati, Sri Dayanti, Efin Windi Devy, Afaf Heny Sintya Dinul, Ahmad Rajul Endang Suprihati Erma Safitri Estoepangestie, Agnes Theresia Soelih Faradina, Putri Laura Febriyatin, Fierdausi Nur’Aini Firdausyi, Aulia Amatul Fransia, Nicola Earren Haditanojo, Wiyanto Hanafi, Ulfiani Fauzia Hani Plumeriastuti Haprabu, Bhima Rochman Satya Harijani, Nenny Hayati, Laila Nur Hermin Ratnani Hidanah, Sri Himawan, Mario Putra Hutahaean, Ryan David Pandapotan IMAM MUSTOFA Ira Sari Yudaniayanti Iskandari, Novia Nur Iwan Sahrial Hamid Izulhaq, Reza Ikhza Khairunnisa, Hanifa Khansa Khasana, Uswatun Kuncorojakti, Suryo Kurnianto, Muhammad Aqil Kusuma, Agung Jati Lestari, Cici Meles, Dewa Ketut Mirni Lamid Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Muzhaffar, Adam Maulana Nanda, Elga Rastika Virya Nasrulloh, Cheisa Alfii Yudha Ningrum, Indah Puspita Nirmalasari, Juwita Ayu Novitasari, Seline Putri Nur Rusdiana Nuraini, Okta Nusdianto Triakoso Oktavianti, Dika Panjaitan, Cahaya Poedji Hastutiek Pradika, Adhitya Yhoga Praja, Ratih Novita Prastika, Dinda Dwi Prastiya, Ragil Angga Primarizky, Hardany Puspitasari, Vina Putri, Lusi Rosita Sartono Putri, Valerie Brilianda Rahmadhani, Nurmitasari Rahmahani, Jola Ratna Damayanti Retno Lastuti, Nunuk Dyah Safitri, Ainun Merta Salsabila Putri, Elma Sandy, Berliana Dwi Nandita Santosa, Gusti Ayu Illiyin Putri Saputro, Amung Logam Saraswati, Adyayutti Wijang Sari, Devi Indah Satria, Rafif Galih Setiawan Koesdarto Sevitasari, Adelita Putri Soeharsono Soeharsono Solikhah, Tridiganita Intan Sri Pantja Madyawati, Sri Pantja Subagyo, Safira Fauziyyah Sukmanadi, Mohammad Susanti, Racca Biana Suwarno Suwarno Tanjung, Aldi Gusnizar Rizaldy Tjuk Imam Restiadi Tri Lestari Tri Wahyu Suprayogi Trilas Sardjito Tyasningsih, Wiwiek Wahjuni, Retno Sri Weliyani, Trima Widya Paramita Lokapirnasari Wilujeng, Estiary Wiwik Misaco Yuniarti Yeni Dhamayanti Yudhana, Aditya Yunita, Maya Nurwartanti Zulianti, Brillia