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Molecular Identification of Lactic Acid Bacteria from Broiler Chicken Meat Mudawaroch, Roisu Eny; Setiyono, Setiyono; Yusiati, Lies Mira; Suryanto, Edi
Agroindustrial Journal Vol 10, No 2 (2023)
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/aij.v10i2.89982

Abstract

Lactic acid bacteria are bacteria used to ferment food. Molecular identification of isolates of lactic acid bacteria has a high degree of accuracy compared to morphological identification. This study aimed to molecularly identify isolates of lactic acid bacteria from broiler chickens. Molecular identification is carried out in 3 stages, namely: rRNA gene amplification stage, squencing and phylogeny tree. PCR electrophoresis results obtained amplicone results measuring about 1,500 bp. The results of BLAST analysis showed that the gen 16S rRNA sequence of BR 17 lactic acid bacteria isolate had a 97-98% similarity with the genome sequence of the Lactobacillus fermentum strain.  Development of phylogenetic tree isolates of single clusters of lactic acid bacteria BR 17 with strains of Lactobacillus fermentum CAU2036, Lactobacillus fermentum JCM 7772, and Lactobacillus fermentum 10-18. The identification results of lactic acid bacteria isolate BR 17 showed Lactobacillus fermentum BR 17.
Low power three phase inverter pure sinusoides output voltage design setiyono, Setiyono
Jurnal Taman Vokasi Vol 11 No 1 (2023)
Publisher : Universitas Sarjanawiyata Tamansiswa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30738/jtvok.v11i1.13236

Abstract

The power conversion from direct current voltage (dc) to alternating current voltage (ac) is called an inverters generally produces a voltage or current waveform that has not approached the sinusoid or in other words, the wave still has ripple or distortion waveform with hight Total Harmonic Distortion (THD )value. One of the factors that influence the resulting inverter waveform is the inverter switchs triggering system. The PWM (Pulse Width Modulation) pulse generation method is a proposed way to solve this problem. To produce a three-phase voltage system, it takes 3 identical power converter circuits but has a different ignition system of 1200 phases for each inverter circuit. The simulation results show that the inverter with an input voltage of 24 V produces three voltage waves of 230/340 V with a Total Harmonic Distortion (THD) content value of 3.565%.
Has Indonesia Safeguarded Traditional Cultural Expressions? Setiyono, Setiyono; Keumala, Dinda; Sabirin, Ahmad; Nursantih, Nadia
Jambura Law Review VOLUME 6 NO. 2 JULY 2024
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33756/jlr.v6i2.24106

Abstract

The Government of the Republic of Indonesia recognized the importance of intellectual property in folklore since the 1982 Copyright Law was enacted (Article 10 of Law No. 6/1982). The study seeks to understand what obstacles Indonesia faces in protecting tribal peoples' communal rights over traditional cultural expressions, also called expressions of folklore in the digital age, and how other nations are resolving them. What is Indonesia's plan for developing and safeguarding traditional cultural manifestations in the digital age, as seen from other nations? The study employs a case-based, normative legal research methodology. The findings, an urgent need for specific institutions that control access benefit sharing from usage by foreign parties, as well as a special anti-theft task force for communal intellectual property rights in the digital era that takes the form of a support organization for tribal peoples. To increase cooperation in the inventorying and documentation of Tribal peoples who own collective intellectual property, optimization also calls for coordination of involvement between the federal government, local governments, and autonomous bodies operating under them, such as the Ministry of Tourism, the Creative Economy Agency, and Tribal peoples' organizations.
Growth of Cut-Grafting Robusta Coffee Seeds Utilizing Orthotropic and Plagiotropic Rootstocks with Application Bacillus and Pseudomonas Mixture Rosyady, Muhammad Ghufron; Larassati, Larassati; Setiyono, Setiyono; Subroto, Gatot; Wijaya, Ketut Anom; Wulanjari, Distiana; Farisi, Oria Alit; Basuki, Basuki
Journal of Applied Agricultural Science and Technology Vol. 8 No. 1 (2024): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v8i1.189

Abstract

Vegetative propagation by cut-grafting has the advantage of being able to obtain seeds that inherit two superior traits from two scion clones in a relatively short time. The purpose of this research is to determine the effect of using orthotropic and plagiotropic rootstocks applied by Bacillus and Pseudomonas on the early growth of robusta coffee seedlings from cut grafting. The method used was by using a completely randomized factorial design with 2 factors and was   repeated 3 times. The first factor was the use of rootstock cuttings which consisted of 2 levels, which were S1 (orthotropic stem) and S2 (plagiotropic stem). The second factor was the concentration of Bacillus and Pseudomonas which consisted of 5 levels, which were B0 (0 ml/L), B1 (20 ml/L), B2 (40 ml/L), B3 (60 ml/L) and B4 (80 ml/L). The results showed that (1) there was no interaction between the use of variatic rootstock and the application of the biological agent (2) The use of orthotropic rootstock increased plant growth, that is the number of primary roots and the number of leaves and (3) Application of the biological agent of Bacillus and Pseudomonas with concentrations of B4 increased seedling growth on all observed parameters except the number of shoots. Based on the results of this research, the benefit for coffee planters is to obtain alternative planting materials, namely from orthotropic branches. For further research, the use of orthotropic branch planting material as a scion plant can be investigated.
Analisis Pembangkit Pulsa Penyalaan Inverter 3 Fasa Menggunakan Space Vector Pulse Width Modulation (SVPWM) Setiyono, Setiyono
Electrician : Jurnal Rekayasa dan Teknologi Elektro Vol. 17 No. 2 (2023)
Publisher : Department of Electrical Engineering, Faculty of Engineering, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/elc.v17n2.2419

Abstract

Paper ini menyajikan algoritma pembangkit pulsa kendali untuk mengatur kinerja saklar inverter sebagai driver motor induksi 3 fasa dengan metode Space Vector Pulse Width Modulation (SVPWM) melalui simulasi pemodelan menggunakan tools matlab Simulink. Deretan pulsa penyalaan dihasilkan melalui kalkulasi beberapa persamaan matematik yaitu perhitungan sinyal referensi, transformasi tegangan 3 fasa abc ke system dua dimensi dq, sudud sector , pola waktu penyalaan saklar inverter, dan pembanding sinyal pembawa segitiga dengan sinyal modulator. Kinerja saklar inverter membentuk 8 kombinasi keadaan dan diilustrasikan memiliki 6 buah sector yang terpisah 60 derajat antar sector. Inverter dua level yang dibangun menggunakan 3 buah pasang saklar semikonduktor MOSFET. Disain simulasi diharapkan dapat dijadikan bahan acuan para peneliti untuk di implementasikan menjadi peralatan pengendalian mesin . Pemodelan ini juga digunakan untuk mengetahui dan menganalisis kendali penyalaan saklar inverter , pengaruh indeks modulasi terhadap kinerja saklar, dan pola waktu penyalaan pulsa tiap tiap pasangan saklar inverter. Hasil simulasi menjelaskan bentuk gelombang pulsa penyalaan yang dibangun menghasilkan deretan pulsa terkendali yang dapat menjalankan performa saklar inverter tiga fasa
Pengaruh Konsentrasi Pupuk STMJ Plus (Susu Telur Molase Jamur Trichoderma sp. dan Limbah Buah-Buahan) terhadap Pertumbuhan dan Hasil Dua Varietas Selada (Lactuca sativa L.): Pengaruh Konsentrasi Pupuk STMJ Plus (Susu Telur Molase Jamur Trichoderma sp. dan Limbah Buah-Buahan) terhadap Pertumbuhan dan Hasil Dua Varietas Selada (Lactuca sativa L.) Ummah, Anisatul; Setiyono, Setiyono; Fanata, Wahyu Indra Duwi; Tanzil, Ahmad Ilham; Arum, Ayu Puspita
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 8 No 2 (2024): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v8i2.465

Abstract

Increasing lettuce production will be done by improving cultivation inputs, such as increased nutritional needs. An alternative organic fertilizer that can be used is made from spoiled milk, eggs, Trichoderma sp. molasses, and fruit waste, or STMJ Plus for short. STMJ Plus Fertilizer acts as an organic nutrient to encourage plant growth. This study aimed to determine the effect of the interaction between STMJ Plus fertilizer concentrations and lettuce varieties on the growth and yield of lettuce plants. The experiment was carried out in a factorial manner using a completely randomized design (CRD) consisting of 2 factors with 4 replications. The first factor is the concentration of STMJ Plus fertilizer consisting of 4 levels: P0: without fertilizer; P1: 5 ml L-1; P2: 10 ml L-1; and P3: 15 ml L-1. The second factor was the variety, which consisted of 2 levels: V1: Grand Rapids and V2: Red Rapid. The results of this study that the interaction of STMJ Plus fertilizer concentrations and lettuce varieties showed highly significant differences in plant height and plant fresh weight variables. The combination that gave the best effect was the concentration of 15 ml L-1 on the Red Rapid (P3V2).
Pengaruh Penambahan Sumber N yang Berbeda Pada Berbagai Media Tanam Terhadap Pertumbuhan Dan Hasil Tanaman Tembakau Kasturi (Nicotiana tabacum) Rafi, Faza Al; Setiyono, Setiyono; Wulanjari, Distiana; Meliala, Susan Barbara Patricia Sembiring
Technologica Vol. 4 No. 1 (2025): Technologica
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/technologica.v4i1.200

Abstract

Tembakau (Nicotiana tabacum L.) memiliki nilai ekonomi tinggi. Untuk memperoleh tembakau yang berkualitas maka media tanam dan dosis pupuk harus diperhatikan dengan baik. Permasalahan produktivitas tembakau ialah belum ada perpadudan yang tepat antara jenis media tanam lain dan pemberian pupuk N. Hal ini penting untuk pertumbuhan dan hasil tanaman tembakau. Studi ini bertujuan mencari kombinasi media tanam yang tepat antara tanah, pasir, serta kompos dan pemberian dosis pupuk urea serta pupuk ZA agar memberikan asupan unsur hara N. Studi ini dilakukan di Bondowoso, Jawa Timur yang dimulai pada November 2023 sampai Maret 2024. Percobaan dilakukan secara faktorial dengan menggunakan pola dasar RAL. Faktor pertama adalah macam media tanam yaitu (M1): tanah, (M2): tanah + pasir, (M3): tanah + kompos + pasir. Faktor kedua adalah jenis pupuk yaitu (P1): pupuk urea, (P2): pupuk ZA. Variabel yang diamati adalah tinggi tanaman, diameter batang, jumlah daun, luas daun, berat daun segar, volume akar dan berat daun kering. Data dianalisis memakai analisis ragam jika ada perbedaan nyata diantara perlakuan maka dilakukan uji lanjutan memakai uji jarak berganda Duncan taraf 5%. Hasil studi menunjukkan interaksi pengaruh media tanam dan jenis pupuk N berpengaruh tidak nyata pada seluruh variabel. Penggunaan berbagai media tanam berpengaruh sangat nyata pada seluruh variabel.  Perlakuan terbaik yaitu kombinasi media tanam tanah pasir dan kompos (M3). Penggunaan berbagai jenis pupuk N berpengaruh tidak nyata pada semua variabel kecuali volume akar.
Response of Vermicompost and Urea Combination Fertilizer Doses on the Growth and Yield of Scallion Plants (Allium fistulosum L.) Fauziah, Fithriyatul; Setiyono, Setiyono; Usmadi, Usmadi; Purnamasari, Ika; Patricia SM, Susan Barbara; Arum, Ayu Puspita; Savitri, Dyah Ayu
International Journal on Food, Agriculture and Natural Resources Vol 5, No 1 (2024): IJ-FANRes
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v5i1.289

Abstract

Leeks (Allium fistulosum L.) are short-lived horticultural crops and have great commercial potential. The productivity of leeks in Indonesia, especially in the East Java region, is experiencing fluctuations, this is due to the problem of decreasing soil fertility. The use of inorganic fertilizers is one of the causes of decreasing soil fertility where the use of inorganic fertilizers in Indonesia reached 105.22 kg/ha, being the highest use compared to several countries in Southeast Asia. Therefore, I offer a solution to this problem, namely by combining the use of inorganic fertilizer with organic fertilizer, for this you can use urea fertilizer and vermicompost fertilizer, where vermicompost fertilizer has advantages compared to other compost fertilizers in terms of complete content starting from microorganisms, growth hormones, and nutrients. The aim of the research I conducted was to determine the interaction of applying urea fertilizer and vermicompost on the growth and yield of leek plants (Allium fistulosum L). The research was conducted at the Green House using a Completely Randomized Design (CRD) with 2 factors, each of which had 3 levels and was repeated 3 times to obtain 27 plant units. The first factor was a vermicompost dose of 0 g/polybag, 75 g/polybag, and 150 g/polybag, while the second factor was a urea dose of 0 g/polybag, 1 g/polybag, and 2 g/polybag. The experimental results showed that the best dose for the combined interaction of vermicompost and urea fertilizer treatment was 75 g/polybag vermicompost and 2 g/polybag urea to increase the number of leaves and height of leek plants.
Effect of Gibberellic Acid (GA3) Concentration and Seedling Media Composition on the Germination and Growth of Shallots (Allium ascalonicum L.) from True Shallot Seed Nurcharisma, Adinda; Setiyono, Setiyono; Sholikhah, Ummi; Ratnasari, Tri; Savitri, Dyah Ayu; Patricia SM, Susan Barbara; Arum, Ayu Puspita; Novijanto, Noer
International Journal on Food, Agriculture and Natural Resources Vol 5, No 1 (2024): IJ-FANRes
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v5i1.274

Abstract

Shallots (Allium ascalonicum L.) is a vegetable commodity that contributes greatly to horticultural production and inflantion levels. The use of superior seeds is expected to produce maximum shallot production. The majority of Indonesian farmers use 30% of the harvested tubers as planting material, with production costs being quite expensive. Many shallot farmers also experience several problems with tuber seeds that are hollow and damaged. True Shallot Seeds can be an efficient and profitable altervative planting material. Implementing TSS shallot cultivation is considered difficult and requires longer time for sowing. Soaking seeds using Gibberelin can have a physiological effect on the germination process. Seedling media factor with a certain composition are needed to produce maximum seeds. The experiment was carried out in factorial manner using Random Design Complete  with three replications. The GA3 concentration factor has four treatment levels: G0 = 0 ppm/control, G1 = 2 ppm, G2 = 4 ppm, and G3 = 6 ppm. The seedling media composition factors has four treatment levels: M0 = soil + compost/control (1:1), M1 = cocopeat + compost (1:1), M2 = husk charcoal + compost (1:1), and M3 = sand + compost (1:1). The data analysis used was Analysis of Variance, if there were significant differences between the treatments, a further tes was carried out using Duncan’s Multiple Range Test at the 5% level. The result showed that the interaction between GA3 concentration treatment and seedling media composition and the single factor GA3 concentration had no significant effect on all observed variables. The composition of the seedling media has a significant effect on the speed of seed growth, seed height, root length, and seed wet weight with optimum results for germination and vegetative growth of TSS seeds, namely the compositionof soil+compost/control (M0) seedling media on the variables of seed growth speed (%/etmal), seed height (cm), and seed wet weight (gram).
Increasing Growth and yield of cucumber (Cucumis sativus L.) by applying baglog waste compost and manure in sand soil and paddy soil combination media Ristiyana, Suci; Saputra, Tri Wahyu; Subroto, Gatot; Setiyono, Setiyono
Jurnal Agrotek Ummat Vol 10, No 3 (2023): Jurnal Agrotek Ummat
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jau.v10i3.15634

Abstract

One of the causes of low productivity in cucumber plants is due to low soil quality due to low organic matter content and high sandy soil mixture. Considering this, it is necessary to try to improve it. So, there is a need for research that examines the addition of organic matter and combines sandy media with paddy soil.This research took place in Pecoro Village, Jember Regency from May 2022 to July 2022. The research method was in the form of a Completely Randomized Design with the first treatment, namely the addition of baglog waste compost and cow manure (K1); baglog waste compost and goat manure (K2); and compost baglog waste and chicken manure (K3). While the second treatment is sandy planting media consisting of: sand 100% (M0); 75% sand + 25% paddy soil (M1); 50% sand + 50% paddy soil (M2); and 25% sand + 75% paddy soil (M3). The observed variables included plant height, total fruit weight, fruit unit weight, fruit length and fruit diameter which were tested by means of variance and Duncan's Multiple Range test.The results showed that the treatment of compost, sandy planting media, and their interactions had a significant effect on the observed variables. The recommendations given based on the results of the analysis for cucumber cultivation are the need to add organic matter such as compost mixed with baglog waste and manure from cow, goat and chicken manure with a ratio of 25% sandy planting medium to 75% paddy soil.
Co-Authors . Usmadi A.A. Ketut Agung Cahyawan W Abdul Rahman Abdullah, Sait Afif, Rendi Ahmad Ilham Tanzil Amam, Amam Amany, Alfiyyah Nur Andrianto, Sis Nanda Kus Anggraini, Febri Arifudin arifudin Arranchado, Jimi Rambo Arta, Roky Adem Arum, Ayu Puspita Az, M. Fariduddin Bakri, Khairani Basuki , Bayuaji, Anandayu Pavita Binti Abd Wahid, Nurul Bahiyah Dewi, Lara Putri Kartika Dewi, Nilasari Dinda Keumala Djoko Soejono Dwi Erwin Kusbianto Dwilaros, Xyzca Dyah Ayu Savitri Edi Pramono Edi Suryanto Eduardus Tandelilin Edy Suryanto Eficandra Eficandra Ely Sufianti Evi Setiawati Falah, Ahmad Naufal Fanata, Wahyu Indra Duwi Fandri, Ferdi Zul Farisi, Oria Alit Fauziah, Fithriyatul Febridayanti, Fika Fitriana, Ananda Gatot Gatot Gatot Subroto Hartaja, Dinda Rita K. Herliani, Canserlita Puteri Hernowo, Arif Heru Dwi Wahjono Hidayah, Satria Nurul Hidayah Ibanah, Indah Ika Purnamasari Innaya, Lutfika Revi Jekti Prihatin Jogiyanto Hartono Joko Purnomo Kadek Wiwik Indrayanti Ketut Anom Wijaya Kunto Wibowo Lies Mira Yusiati Maharani, Ariq Dewi Mamduh M. Hanafi Mega Fatimah Rosana Megouw, Edwin Wakak Meliala, Susan Barbara Patricia Sembiring Muchammad Azam, Muchammad Muchlison, Wildan Mudawaroch, Roisu Eny mudawaroh, roisu Muhammad Fahrurozi Nahdiya Sabrina Napitupulu, Randy Nisak, Fauziatun Noer Novijanto Noor, Risyadilla Amalia Noviana, Sulfi Novita Listyaningrum, Novita Nugraha, Chairul Fattah Nugraha, Yosep Widi Nurcharisma, Adinda Nurrahmi, Tyas Putri Yulia Nursantih, Nadia Okti, Reski Dwi Pajuk, Daniel Miranda Patricia SM, Susan Barbara Patricia SM., Susan Barbara Patricia, Susan Barbara Petrus Nugro Rahardjo Pramesti, Riska Ayu Priyanto, Fajar Eko Purba, Novalina Putri, Natasya Stivani Putri, Sabrina Claudia Anggraini Rachmandhika, Yusuf Rafi, Faza Al Rahmat, Nur Ezan Ramadhanti, Choirul Lisa Ratnasari, Tri Rena Yunita Rahman, Rena Yunita Renie, Elsy Ridho, Mavel Ristiyana, Suci Robertus Haryoto Indriatmoko Romdhon, Mochammad Syihab Rony, Zahara Tussoleha Rosania, Shela Roswan, Roswan Rosyady, Muhammad Ghufron Rudi Nugroho Sabirin, Ahmad Salma, Melza Ayu Saroh, Cindy Nur May Satmoko Yudo Sa’idah, Nely Meliatus Sholeh Avivi Slameto Slameto Soemitro Djojowidagdo Sri Hartatik SRI RAHAYU Sugeng Raharto Sukarno, Anggo Kusuma Sukatin, Sukatin Supartono, Sugeng Susi Sulistia, Susi Suwardiyanto Suzaini, Syaqila Binte Titik Purwati Widowati, Titik Purwati Tri Wahyu Saputra Ubaidillah, Mohammad Ulinnuha, Firda Ummah, Anisatul Ummi Sholikhah Utami, Intan Rahmatika El Putri Warman, Arifki Budia WATI, RAHAYU SARAS Wicaksono, Herlambang Widya Asmara Wiwik Handayani Wulanjari, Distiana Yuli Hariyati Yuliawati, Eriyana Yuliawati, Eriyana Zahrosa, Dimas B. Zahrosa, Dimas Bastara Zainuddin, Zainuddin