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DNA Barcoding of Red Algae (Rhodophyta) in Ternate Island Sea, North Maluku, Indonesia Achmad, Muhammad Janib; Akbar, Nebuchadnezzar; Ismail, Firdaut; Samman, Ardan; Subhan, Beginer; E Paembonan, Rustam; Arafat, Dondy
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.44436

Abstract

Abstract Ternate Island is located on the North Maluku Sea. The North Maluku Sea region includes the Wallacea area and the world's coral triangle. One of the organisms commonly found in this sea is red algae. Study aimed to determine the red algae species by phylogenetic tree analysis based on the rbcL gene as a DNA marker. The preserved red algae tissue samples were extracted with the Geneaid GP100 DNA Extraction Kit Plant. The DNA sample was amplified and then visualized by 1% agarose gel electrophoresis. The amplicon products were sequenced and then aligned with the rbcL gene database that was available at the NCBI gene bank. The phylogenetic tree was constructed using the UPGMA method. The results showed that red algae were identified into four species: namely Gibsmithia hawaiiensis (98.65%), C_rbcL sample was identical to Amansieae sp. (91.50%), D_rbcL sample was identical to Peyssonnelia sp. (95.54%), and G_rbcL sample was similar to Portieria hornemanniI (96.15%). Based on phylogenetic tree analysis, Gibsmithia hawaiiensis from North Maluku is closely related to species from Raja Ampat, West Papua, Indonesia, followed by the Philippines species. Special findings were found to carry out phylogenetic reconstructions that can answer inter-species kinship. The general finding is that the markers used can be used for phylogenetic construction. Phylogenetic construction of Peyssonnelia sp. in North Maluku is related to species from South Africa. North Maluku's Portieria hornemannii is closely related to a species from Korea. Highlight Research This study provides initial information about the DNA barcoding of red algae. Important for management purposes and determining the status of red algae in the future. This scientific information also provides an understanding of the relationships among red algae species in the world's oceans. Global warming has changed the temperature of seawater and affected the population of marine organisms.
High and Low Taxa Specificity of Rhizosphere Bacteria Communities of Mangrove (Rhizophora mucronata) from Kuala Langsa and Telaga Tujuh Island, East Aceh Maysaroh, Siti; Samira Ismet, Meutia; Andini, Rita; Anggraini, Nurlita; Subhan, Beginer
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.55882

Abstract

Highlight Research Bacteria from mangrove sediment have been identified, and environmental parameters have been analyzed. The relationship between rhizosphere bacteria and the environment has been analyzed. The distribution and abundance of rhizosphere bacteria differ between Kuala Langsa and Telaga Tujuh. The diversity and abundance of rhizosphere bacteria are not influenced by environmental parameters. Abstract The focus of this study on understanding the structure and dynamics of rhizosphere bacteria in mangrove ecosystems is driven by the increasing acknowledgment of the crucial roles these microorganisms play in ecosystem functioning. Rhizophora mucronata, a key mangrove species, is known for its ecological significance. Investigating the bacteria associated with its rhizosphere offers valuable information about the symbiotic relationships between mangrove vegetation and microbial communities. Bacteria are vital for decomposition and nutrient availability in mangroves. This research examines sediment from rehabilitated and natural areas to understand how human and natural factors shape bacterial communities.The DNA sequence was analyzed using Next-Generation Sequencing (NGS), which targeted the 16S bacterial region in the V3-V4 rDNA. Additionally, environmental factors such as nitrate, phosphate, and sulfur content were also analyzed. Kruskal-Wallis and T-test statistical analyses were used to examine the abundance of bacteria and environmental parameters between study sites.There are differences in the types of bacteria found in Kuala Langsa and Telaga Tujuh. Approximately 7% of the rhizosphere bacterial groups were exclusively detected in Telaga Tujuh, such as Fusobacteriia (Class). Additionally, the abundance of bacteria at both locations differs significantly (p < 0.05), as determined by Kruskal-Wallis. The results of the t-test indicate that the observed environmental parameters do not differ significantly from each other. The environmental parameters studied did not significantly impact the types or abundance of detected rhizosphere bacteria.
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Maysaroh, Siti; Ismet, Meutia Samira; Subhan, Beginer; Andini, Rita; Sembiring, Enny Rimita; Anggraini, Nurlita Putri
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Phytoplankton in Eutrophic Waters of Hurun Bay: An Examination of Composition Patterns, Structure, and Impacts on Aquatic Ecosystems Munru, Maestro; Ismet, Meutia Samira; N.Natih, Nyoman Metta; Subhan, Beginer; Aziz, Nasrul
International Journal of Marine Engineering Innovation and Research Vol 9, No 3 (2024)
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25481479.v9i3.21625

Abstract

Ecological conditions are shifting due to increased anthropogenic activities along coastal areas. These activities are affecting the life cycle of aquatic organisms, especially phytoplankton. This study, conducted in the unique ecosystem of Hurun Bay Lampung, aims to analyze the composition and structure of phytoplankton communities in eutrophic waters and water quality conditions. Sampling was conducted in two different months, August and October, by vertical hauling from a depth of 5 meters to the surface. The results revealed a rich diversity of phytoplankton, with 72 genera, 48 families, and 6 classes identified in August, and 55 genera, 42 families, and 6 classes identified in October. The diatom group was the most prevalent, accounting for over 90% of the total, with the highest abundance found in Skeletonema and Chaetoceros. The diversity, uniformity, and dominance indices were all in the low to medium category, indicating a balanced ecosystem
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Maysaroh, Siti; Ismet, Meutia Samira; Subhan, Beginer; Andini, Rita; Sembiring, Enny Rimita; Anggraini, Nurlita Putri
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Perhitungan Cepat Kelimpahan Acanthaster planci di Pulau Sombori, Morowali, Sulawesi Tengah Zamani, Nevyati P.; Subhan, Beginer; Rachma ati, Rita; Jansit, Afifah F.
Jurnal Sumberdaya Akuatik Indopasifik Vol 8 No 1 (2024): Februari
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46252/jsai-fpik-unipa.2024.Vol.8.No.1.353

Abstract

Acanthaster planci is one of the most potential causes of coral reef damage in Indonesia. The presence of A. planci in Indonesia has been reported with a large population and preys on corals with large numbers. This research as conducted in order to provide information quickly related to the category of A. planci hems in the Sombori tribe. The monitoring method used is the time s imming method hich is carried out for 2x10 minutes. Categorization of outbreak determination using threshold approach developed in the ORANET (Oceania Regional Acanthaster Network) program. Monitoring the abundance of A. planci as carried out in seven locations with 10 observation points, namely Dongkalan Kecil Island with 123 individuals, Dongkalan Besar Island with 5 individuals, Mbokita Island with 60 individuals, Kayangan Island with 7 individuals, Red Sand Beach with 5 individuals, Air Kiri Beach with 6 individuals, and Goa Alo Beach with 6 individuals. The high number of A. planci individuals is only found on 2 islands, namely Dongkalan Kecil Island and Mbokita Island. The number of A. planci on these t o islands is included in the category of possible outbreaks and is included in the category of threatening coral reef ecosystems. The condition of coral reefs on Dongkalan Island is small dominated by dead corals thought to be due to predation of A. planci. hile on Mbokita Island, the condition of coral reefs is not dominated by dead corals but there are many remnants of predation on corals. 
Ekologi dan Dimensi Pengelolaan Keberlanjutan Mangrove (Studi Kasus Lokasi Ekowisata Guraping, Kota Tidore Kepulauan, Maluku Utara) Paembonan, Rustam Effendi; Akbar, Nebuchadnezzar; Ibrahim, Abjan; Tahir, Irmalita; Baksir, Abdurrachman; Marus, Ikbal; Najamuddin, Najamuddin; Ismail, Firdaut; Wibowo, Eko S; Siolimbona, Abdul Ajiz; Harahap, Zulhan Arifin; Mutmainnah, Mutmainnah; Angkotasan, Abdul Motalib; Zamani, Neviaty P; Arafat, Dondy; Subhan, Beginer; Natih, Nyoman MN; Rahman, Rahman; Rahimah, Insaniah; Harahap, Zulham Apandy
Jurnal Sumberdaya Akuatik Indopasifik Vol 8 No 4 (2024): November
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46252/jsai-fpik-unipa.2024.Vol.8.No.4.412

Abstract

The mangrove ecosystem is a potential coastal and marine area that has a very important resource function. The mangrove ecosystem must be managed sustainably so that it can provide optimal benefits, especially the mangrove ecosystem in Guraping Village which has been designated as an ecotourism area. The aim of this research is to analyze the ecological condition of the mangrove ecosystem and analyze the dimensions of sustainable management of the mangrove ecosystem in Guraping Village. The method used in this research line transect method with a 10x10 meter quadrant to determine ecological conditions. Interviews were conducted with key respondents from stakeholders and the dimensions of sustainable management were analyzed using Rapfish. Measurement of environmental parameters directly on site. The results obtained from this research can determine the ecological condition of mangroves at the research location which still have good status based on the number of species, species density, species cover, diversity index, mangrove canopy cover data. The calculation of the results of the assessment of the dimensions of mangrove sustainability management, which consists of ecological dimensions, economic dimensions, socio-cultural dimensions, legal, institutional and policy dimensions and technological and infrastructure dimensions, is classified as a less accountable level category.
Antioxidant of Ethanol Extract and Toxicity of Fractions from Aspergillus unguis a Marine Sponge Symbiont of Aaptos suberitoides Masyerli, Scify Bilqis Nawafi; Efdi, Mai; Bashari, Muhammad Hasan; Untung Kurnia Agung, Mochamad; Subhan, Beginer; Efahmi, Efahmi; Andriani, Yosie; Syafrizayanti, Syafrizayanti
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 1 (2025): February 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i1.14645

Abstract

The marine sponge Aaptos suberitoides has been shown to have anticancer properties, with clear evidence of its capacity to suppress the growth of cancer cells. However, the pharmaceutical exploration of chemicals from marine organisms causes significant environmental concerns. Aspergillus unguis, a fungal symbiont of the marine sponge A. suberitoides, has been isolated for its potential in sustainable natural products resources. This study assesses the antioxidant activity of ethanol extract and the toxicity of four levels fractions from ethanol extracts of A. unguis mycelium and these results were reported for the first time in this study. The antioxidant of ethanol extract was determined using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The fractions were examined for toxicity using the Brine Shrimp Lethality Test (BSLT). The ethanol extract of A. unguis shows very strong antioxidant bioactivity (IC50 = 42.84 mg/L). The LC50 values for hexane, chloroform, ethyl acetate, and butanol fractions were determined to be 74.11 µg/mL, 93.84 µg/mL, 59.37 µg/mL, and 142.79 µg/mL, respectively. It indicates significant toxicity. These preliminary results are important knowledge for further research into the bioactivity potential of the metabolites as candidate anticancer compounds, aligning with marine pharmaceutical drug development.
Potensi aktivitas antioksidan ekstrak spons laut Stylissa carteri: Potential antioxidant activity of sea sponge Stylissa carteri extract Adewal, Murad Alvian K; Abdullah, Asadatun; Zamani, Neviaty Putri; Arafat, Dondy; Ayu, Inna Puspa; Subhan, Beginer
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 3 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i3.60270

Abstract

The sea sponge Stylissa carteri plays an important role because it is one of the constituent biota of coastal and marine ecosystems, especially in coral reef ecosystems. The increasing utilization of bioactive compounds in sea sponges is feared to significantly reduce the population of sea sponges. The prevention effort involves cultivating sea sponges for sustainable utilization. This study aims to determine the antioxidant activity of transplanted Stylissa carteri sponges and those that live naturally in nature. This study was conducted in Pramuka Island using the sponge explant combination method. The three types of transplants were number one (SO), number two (S. carteri and Aaptos suberitoides), and number three (S. carteri, A. suberitoides, and Acropora hard coral). Antioxidant activity was tested with three repetitions on three transplanted and naturally occurring S. carteri sponges (SA) using the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) method. The results indicated that the four sample sources contained strong antioxidant activity, namely SO had an IC50 value of 86.22±23.6 μg/mL, SAP 86.58±30.7 μg/mL, SAC 86.10±23.4 μg/mL, and natural living SA 54.27±16.9 μg/mL. The transplanted S. carteri sea sponge has strong antioxidant activity and can be an alternative solution in terms of raw material availability for the development of potential pharmaceutical products.
POLA KEANEKARAGAMAN DAN DISTRIBUSI SPESIES IKAN TERUMBU MENGGUNAKAN ENVIRONMENTAL DNA (eDNA) METABARCODING PADA JARAK LONGITUDINAL DARI TELUK JAKARTA Sani, Lalu M Iqbal; Benyamin, Azhari; Husna, Alief K; Arafat, Dondy; Subhan, Beginer; Sunuddin, Adriani; Cakasana, Nadya; Lestari, Dea Fauzia; Madduppa, Dr. Hawis
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 13 No. 3 (2021): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v13i3.37971

Abstract

Keberadaan ikan terumbu tentunya erat kaitannya dengan keberadaan terumbu karang karena ekosistem tersebut merupakan habitat bagi ikan karang. Terumbu karang merupakan ekosistem yang banyak terdapat di pulau-pulau kecil di daerah tropis termasuk Kepulauan Seribu. Kepulauan Seribu adalah sekelompok 110 pulau yang terletak di lepas pantai Jakarta dan hingga 80 kilometer sebelah utara Laut Jawa. Dalam studi ini, kami meneliti distribusi spesies dan keanekaragaman spesies ikan terumbu pada dua lokasi yaitu Pulau Untung Jawa dan Pulau Harapan yang berbeda jarak di Teluk Jakarta menggunakan analisis metabarcoding DNA lingkungan (eDNA). Sebanyak 4 liter sampel air laut dikoleksi pada kedalaman 8-9 meter per lokasi lalu dilakukan analisis menggunakan primer spesifik (MiFish U) dengan marka 12s rRNA. Secara keseluruhan, kekayaan spesies yang lebih tinggi ditemukan di Pulau Harapan (52 spesies) diikuti oleh Pulau Untung Jawa (11 spesies). Indeks Shanon-Wiener juga menunjukkan bahwa Pulau Harapan memiliki keanekaragaman ikan terumbu yang lebih tinggi berdasarkan tiga tingkatan taksonomi (famili, genus, dan spesies). Hanya ada lima spesies ikan terumbu mutual yang ditemukan di dua lokasi tersebut, yakni Atherinomorus aetholepis, Auxis thazard, Cephalopholis sexmaculata, Epinephelus chlorostigma, dan Plectropomus areolatus. Hasil temuan dalam penelitian ini sejalan dengan perbedaan antropogenik dimana Pulau Untung Jawa lebih dekat dengan Teluk Jakarta dibandingkan dengan Pulau Harapan yang letaknya relatif jauh dari Teluk Jakarta.
Co-Authors Abdul Motalib Angkotasan Abdurrachman Baksir Abdurrachman Baksir, Abdurrachman Abjan Ibrahim, Abjan Abubakar, Salim Achmad, Muhammad Janib Adewal, Murad Alvian K Aditya Bramandito ADRIANI ADRIANI Adriani Sunuddin Afiff , Usamah Agus Ramli Ahmad Eko Suprianto Ahmad Taufik Ghozali Ahmad Taufik Ghozali Aisyah, Siti Zanuba Aji Wahyu Anggoro Alan Frendy Koropitan Ali Mashar Aliati Iswantari Alla Asmara Am Azbas Taurusman Andhika Prima Prasetyo Andrianus Sembiring Anggi Mayulina Daulay Anggraini, Nurlita Anggraini, Nurlita Putri Anwar, Muhammad Fathur Aradea Bujana Kusuma Aradea Bujana Kusuma Ardan Samman Arif Miftahul Aziz, Arif Miftahul Ario Damar Asadatun Abdullah Asep Sandra Budiman, Asep Sandra Ayu Ervinia Ayu, Inna Puspa Aziz, Nasrul Bashari, Muhammad Hasan Benyamin, Azhari Bertoka Fajar S.P. Negara Borbee, Erin Budi Prabowo Citra S. U. Dewi Citra Satrya Citra Satrya Utama Dewi Dea Fauzia Lestari, Dea Fauzia Dedi Soedharma Dedi Soedharma Denny Khaerudi Dewi, Citra Satrya Utama Diah Ayuningrum, Diah Dian Pertiwi Dietrich Geoffrey Bengen Dietrich Geoffrey Bengen Dietriech G Bengen Dietriech Geoffrey Bengen Dondy Arafat Dondy Arafat Dwi Yuni Wulandari E Paembonan, Rustam Efahmi, Efahmi Eko S Wibowo Elfahmi Elfahmi, Elfahmi Elok Faiqoh Endang Sunarwati Srimariana Fadel Muhammad Fadhilah Rahmwawati Fadillah Rahmawati Fahlevi, Arief Reza Fajar Maulana . Fauzan Dzulfannazhir Fauzan Dzulfannazhir Febriantika, Putri Fenetiruma, Syamsudin Firdaut Ismail Firdaut Ismail Firsta Kusuma Yudha Gelis, Ester R. E. Genadi Algadri Hardianti, Aprilia Hari, Restu Fajar Hawis H Madduppa Herettrenggi, Rosa Hermansyah Amir, Hermansyah Husna, Alief K I Wayan Nurjaya Idham A. Nasrullah Ikbal Marus Ikbal Marus, Ikbal Ilham, Yuwanda Inna Puspa Ayu Irianda, Nadya Jeny Irmalita Tahir Irmalita Tahir, Irmalita Irnin Agustina Dwi Astuti Islami, Nurfara Ismail, Firdaut Iswandi Wahab Jansit, Afifah F. Jeddah Isnul Jhoni Wahyu Adi Joko Santoso Junaedy, Ahmad Kahar, Fauzi Abdul Karizma Fahlevy Khairudi, Denny Kintani, Novia Indah Kotta, Raismin Kusumarani, Dheni Lalu M. Iqbal Sani Lalu M. Iqbal Sani Lane, Christopher Luzmi Malia Izza Maestro Munru Mai Efdi Majariana Krisanti Malaihollo, Jantje Jacobus Manafi, Efi Noferya Mas Irfanto Masyerli, Scify Bilqis Nawafi Meutia Samira Ismet Minsaris, La Ode Alam Miswandi, Muhammad Mohamad Gazali Mohammad Mukhlis Kamal Muhamad Gilang Arindra Putra Muhamad Gilang Arindra Putra Muhamad Iqbal Muhammad Iqbal Muhammad Irfan Muhammad Janib Achmad Muhammad Taufik Muhammad Yusuf Muhandis Shidqi Munru, Maestro Mutmainnah Mutmainnah Muttaqin, Ahmad Sabilul Mu’min Mu’min N Natih, Nyoman Metta N, Furqan Nadya Cakasana Nadya Jeny Irianda Najamuddin Najamuddin, Najamuddin Nasrul Aziz Nebuchad Nezzar Akbar Nebuchadnezzar Akbar Nebuchadnezzar Akbar Neviaty P. Zamani NEVIATY PUTRI ZAMANI Ni Kadek Dita Cahyani, Ni Kadek Dita Niken TM Pratiwi Ningtyas, Azizah Lutfia Nor Jannah, Nor Novriyandi Hanif Nur Ari Bayu Nurlisa Alias Butet Nurlita Anggraini Nurlita Putri Anggraini Nurlita Putri Anggraini Nyoman M N Natih Paembonan, Rustam E Prakas Santoso Prakas Santoso Prakas Santoso Prakas Santoso Prehadi . Prehadi Prehadi Prehadi, Prehadi Purwanto, Budi Rachma ati, Rita Rahamadya, Aldiano Rahimah, Insaniah Rahman Rahman Rahmat Hidayat Rahmat, Fadhlan Basiluddin Ramadian Bachtiar Ramili, Yunita Rani Utari Ayuningtyas Rasidi, Rasidi Raunsai, Jhoni Elias Raweyai, Oktofianus Obaja Restu, Yunan Gilang Ridha Fathihatunnisa Rita Andini RITA ANDINI Riyadi Subur, Riyadi Rommy M. Abdullah Rotinsulu, Dordia Anindita Rustam E Paembonan Rustam E Paembonan Rustam Effendi Paembonan, Rustam Effendi Ryan Adhitia Muslim Safika S, Safika Samira Ismet, Meutia Samsuardi Samsuardi, Samsuardi Sani, L. Mukhsin Iqbal Sani, Lalu M Iqbal Sani, Lalu M. Iqbal Sani, Lalu Mukhsin Iqbal Santoso Budi Widiarto Satya Pratama Atmaja Sebastian C. A. Ferse Sebastian Ferse Sembiring, Enny Rimita Setiamarga, Davin H. E. Siolimbona, Abdul Ajiz SITI KHODIJAH Siti Maysaroh Siti Maysaroh Sombo, Hendrik Sukandar Sukandar Sukandar, Mr. Syafrizayanti, Syafrizayanti Syahid Syamsul Bahri Agus, Syamsul Bahri Tumpak Sidabutar Ulandari, Rafika Untung Kurnia Agung, Mochamad Wahab, Iswandi Wedi Andika Wibowo, Eko S Wibowo, Eko Setyobudi Wiwid Andriyani Lestariningsih Yahya, Rodhiyah Yennafri, Yennafri Yidoatimojo, Sudibyo Yoga Budi Bhakti yoman Metta N Natih Yosie Andriani Yuliana Fitri Syamsuni Yusli Wardiatno Zamani, Nevyati P. Zedta, Raymon Rahmanov Zulham Apandy Harahap Zulhan Arifin Harahap