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Revealing Porifera Diversity by Metabarcoding Environmental DNA Data in Coral Reef Ecosystems of Lombok Island Waters Arief Pratomo; Jelita Rahma Hidayati; Carolline Desrainy Nababan; Beginer Subhan; Inna Puspa Ayu
Journal Omni-Akuatika Vol 21, No 2 (2025): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2025.21.2.1226

Abstract

Lombok waters are located in the Coral Triangle area, a region with high marine biodiversity, including Porifera. Information on Porifera diversity in Lombok waters is still minimal, due to the difficulty of identifying them. Metabarcoding eDNA is an alternative method for revealing the potential diversity of Porifera non-invasively, widely, quickly, and effectively. This study aimed to determine the diversity of Porifera with eDNA data in Lombok Waters. This study used quantitative methods with bioinformatics tools in the data processing process. The eDNA data were obtained from previous research results that have been stored in GenBank PRJNA768103 which includes seawater and sediment sample types in the 12 µm and 0.4 µm membrane pore fractions in the East, West, and North Lombok regions This study successfully identified 19 genera of Porifera dominated by Hippospongia, Haliclona, and Terpios in each location and sample type. Comparison of alpha diversity showed a significant difference in membrane pore fraction (p = 0,0262). Meanwhile, beta diversity showed a significant difference in the Porifera community between East and North Lombok (p = 0,01). Significant differences in alpha and beta diversity indicate the influence of environmental factors and habitat characteristics in shaping the community structure of Porifera in Lombok waters. These findings highlight the potential of the eDNA approach as an efficient biomonitoring tool for marine biodiversity and support sustainable management and conservation of Lombok’s marine ecosystems.
Mapping and validation of spatial algorithm for monitoring turbidity of seagrass habitat using sentinel-2B imagery in Ternate Island Rustam Effendi Paembonan; Dietriech Geoffrey Bengen; I Wayan Nurjaya; Syamsul Bahri Agus; Nyoman Metta N Natih; Beginer Subhan; Joko Santoso
Depik 2025: Special Issue ICMF
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.0.0.46989

Abstract

Turbidity is a parameter of the marine environment that greatly affects the condition of seagrass whose habitat is an intertidal zone in shallow sea waters. Seagrass is an important type of ecosystem that can be found in several coastal areas of Ternate Island. This study aims to analyze the turbidity conditions of seagrass habitat waters and apply a remote sensing algorithm using Sentinel 2B images. The turbidity research method was carried out by field measurements. The turbidity algorithm model used refers to references with mathematical equations (Rrs665-0.014)/0.013, and the development of a new algorithm as a comparison algorithm. Both algorithms were validated with field data to determine the level of accuracy using the Normalized Mean Absolute Error (NMAE) and determination coefficient (R2). The results were obtained from turbidity data with values ranging from 0.3 NTU to 1.5 NTU with an average value of 0.87 0.45 NTU. The Sentinel 2B image in this study was restored with geometric corrections, atmosphere, radiometric digital values, land masking, and sun glint. The turbidity algorithm model used obtained good accuracy in mapping and monitoring the turbidity of seagrass habitat waters on Ternate Island. The application of the turbidity algorithm used as a reference in this study has an NMAE value of 50.44 and R2 of 0.8822, while the newly discovered turbidity algorithm has an NMAE value of 29.38 and R2 of 0.8827.Keywords:remote sensingecosystemscoastalsedimentationNorth Maluku
Preliminary study oceanography and environmental parameters of habitat endemic species Halmahera walking shark (Hemiscyllium halmahera) on Indopacific Ocean Area in North Maluku Indonesia Nyoman Metta N Natih; Nebuchadnezzar Akbar; Dietriech G Bengen; I Wayan Nurjaya; Riyadi Subur; Abdurrachman Baksir; Firdaut Ismail; Iswandi Wahab; Dondy Arafat; Rustam E Paembonan; Abdul Ajiz Siolimbona; Rasidi Rasidi; Asep Sandra Budiman; Beginer Subhan
Depik 2025: Special Issue ICMF
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.0.0.46913

Abstract

The Halmahera Walking Shark (Hemiscyllium halmahera) is an endemic species in the Halmahera Sea. Information related to the environment in general has not been described in a complex manner. The environment is the main factor to support the life of biota. Environmental characteristics are a determining factor in the sustainability of biota. The study aims to provide information related to the physical environment of the habitat of the H. halmahera species. Primary data collection was carried out directly (in situ). Current data was taken using the Lagrange method using a currents drogue. Tides using tidal palms and observed for 24 hours. Secondary data was taken from Marine Copernicus and the Geospatial Information Agency (BIG). All data were tabulated and analyzed by Ocean Data View (ODV) software. The results of the study found that the surface current velocity was found to be in the fast current category. Current speed descriptively provides information on the life of H. halmahera. Primary data shows that the direction of the surface current moves to the east and north. Secondary data in vertical distribution shows weak zonal and meridional current shifts. The tidal pattern was found to be two high and low tides (semidiurnal). The distribution of surface temperature was found to be around 30-32oC. The vertical temperature profile found three layers, namely 1-13m (30oC), 65m (25oC) and 71m (20oC). The chemical environment showed all normal variables. The physical and chemical environmental conditions support H. halmahera to inhabit the sea area of Morotai Island. The environmental description has provided a brief understanding of how the ecological role of the H. halmahera species. Management and sustainability of the environment in the future due to climate change can be done with information on habitat characteristics.Keywords:coral triangleHalmaheranicheNorth Malukutropical
Condition and zonation of mangrove ecosystems in the small islands around the area crossed by the equatorial line of North Maluku Province Najamuddin Najamuddin; Abdurrachman Baksir; Nebuchadnezzar Akbar; Firdaut Ismail; Abdul Ajiz Siolimbona; Dondy Arafat; Rustam E Paembonan; Raismin Kotta; Beginer Subhan; Irmalita Tahir; Nyoman MN Natih; Eko S Wibowo; Ikbal Marus; I Wayan Nurjaya; Dietriech Geoffrey Bengen
Depik Vol 13, No 2 (2024): AUGUST 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.2.32075

Abstract

The South Halmahera Islands feature a comprehensive marine coastal ecology. The ecosystems found were seagrass, mangrove and coral reef ecosystems. The components that sustain the existence of this ecosystem are employed equitably because of its geographical location on the equator. Mangrove ecosystems that provide benefits directly and indirectly to the community. The purpose of this study was to determine the condition of the mangrove ecosystem based on the hemispherical photography method and the type of zonation. Tawabi Island, Kayoa, South Halmahera Regency served as the site of the equator research. The research station is determined as many as 3 stations. Mangrove percent cover canopy using the hemispherical photography and transect quadrant for density. Field data gathering procedures with transects drawn perpendicular from the sea to the land. Zonation characteristics and thickness using zone tracking. Density mangrove using ecology analysis and zonation using canvas 9.0. The results of the research founded were Rhizophora apiculata, Rhizophora mucronata and Sonneratia alba. Mangrove percent cover canopy were good category (75-81%), where station I (75%), station II (81%) and station III (77%). Station I having 600 trees per ha, station II having 500 trees per ha, and station III having 400 trees per ha (low density category). Mangroves were found in 3 zones Sonneratia alba, Rhizophora apiculata and Rhizophora mucronata. The mangrove thickness from sea to land at station I (65 meters), station II (69 meters) and station III (42 meters).Keywords:biodiversitycoastalequator lineHalmaheratropical
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Siti Maysaroh; Meutia Samira Ismet; Beginer Subhan; Rita Andini; Enny Rimita Sembiring; Nurlita Putri Anggraini
Depik Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Morphological identification of the soft coral, Clavularia inflata, reveals different sclerite characters across Indonesian coral reefs Beginer Subhan; Dietriech G Bengen; Sebastian Ferse; Fauzan Dzulfannazhir; Nurlita Putri Anggraini; Prakas Santoso; Dondy Arafat; Lalu M. Iqbal Sani; Prehadi Prehadi; Hawis Madduppa
Depik Vol 11, No 2 (2022): August 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.2.22102

Abstract

Soft corals of the genus Clavularia (Blainville 1830) have complex and difficult to distinguish morphological characteristics, making them rather difficult to identify. A paucity of information exists for Indonesian coral reefs, a complex biogeographic region at the confluence of the Pacific and Indian Ocean that covers an extensive geographic area and several marine ecoregions, is subject to several ocean current systems, and includes a high diversity of habitats. Specimens of Clavularia inflata were collected from eleven study sites across the Indonesian archipelago, morphologically described based on their sclerite characteristics, and compared among sites. Sclerite observations were divided into four parts for each colony, namely pinnules, crowns, calyces, and stolons. The sclerites found in C. inflata comprised various forms such as rods, fingers, sticks, and brackets. Morphometric measurements of C. inflata showed specimens from Kepulauan Seribu had the largest pinnule, calyx, and stolon sclerites compared to other locations. The maximum mean size of crown sclerites was observed for specimens from Maluku Tenggara Barat. Similarities in the shape of sclerites resulted in specimens from Kangean, Morotai, Tanjung Lesung, Bontang, Lombok and Natuna forming a cluster, while those from Maluku Tenggara Barat and Arborek were dissimilar to other sites. Smaller sclerite sizes in specimens from Arborek are likely related to strong currents at that site. In conclusion, there were variations in the size of sclerites across the eleven study sites, with several sites forming a cluster of similar sclerite dimensions. Further studies using molecular markers are suggested to further explore the possibility of cryptic species.
Membangun Indikator Keberhasilan Restorasi Terumbu Karang Beginer Subhan; Dondy Arafat; Inna Puspa Ayu; Nebuchadnezzar Akbar
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol. 7 No. 4 (2025): Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0704.1442-1447

Abstract

Restorasi terumbu karang adalah serangkaian upaya ilmiah dan manajerial untuk memulihkan ekosistem terumbu karang yang rusak akibat faktor alam dan aktivitas manusia. Teknik restorasi yang digunakan termasuk transplantasi karang, pembuatan terumbu karang buatan, dan pengendalian stresor lokal seperti polusi, overfishing, dan perubahan iklim. Kegiatan restorasi di Indonesia telah dilaksanakan di lebih dari 500 titik dalam 30 tahun terakhir, melibatkan ribuan unit terumbu buatan dan jutaan fragmen karang. Namun, banyak dari kegiatan ini tidak menunjukkan keberhasilan yang ditandai dengan kegagalan.  Faktor peningkatan suhu laut, persaingan dengan alga atau spons, serta faktor manusia menjadi penyebab utama. Saat ini, Indonesia belum memiliki standarisasi untuk menilai keberhasilan restorasi terumbu karang. Oleh karena itu, penting untuk mengembangkan parameter dan indikator yang dapat digunakan untuk menilai efektivitas dan keberhasilan kegiatan restorasi, guna memastikan keberlanjutan ekosistem terumbu karang.
DETECTING GROUPER (EPINEPHELINAE) DIET COMPOSITION AND PREY AVAILABILITY IN RAJA AMPAT CORAL REEFS THROUGH DNA AND eDNA METABARCODING Hawis Madduppa; Dietriech Geoffrey Bengen; Inna Puspa Ayu; Beginer Subhan; Lalu M Iqbal Sani; Budi Prabowo
BIOTROPIA Vol. 30 No. 1 (2023): BIOTROPIA Vol. 30 No. 1 April 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.1.1608

Abstract

Gaining extensive knowledge of prey sources is an essential approach for understanding trophic structure and relationships, especially in highly diverse coral reef ecosystems. Groupers are a major Asian reef fish commodity, making it important to study grouper prey and the trophic relations involved. The wide distribution of groupers across different environments could lead to distinctive predatory behaviour. Therefore, this study was conducted to investigate and compare the taxonomic classification and composition of prey in the diets of two common groupers (Epinephelus areolatus and E. malabaricus) based on DNA metabarcoding of stomach contents and potential prey detection using environmental DNA tools at sites in the coral reefs of Raja Ampat, Indonesia. DNA recovered from the water column comprised taxa from the Arthropoda, Chordata, Cnidaria and Mollusca, several of which were also found in grouper guts, with Cnidaria the most abundant class. Diversity was high for potential prey species in the environment and prey consumed by each grouper species. The high overlap in prey identified from gut contents indicates these two epinephelids have a similar feeding strategy. However, nMDS ordination showed segregation between the prey consumed by each species and potential prey available in the environment. The results indicate a low likelihood of competition between the two grouper species, related to the abundance and wide choice of potential prey in the highly biodiverse Raja Ampat coral reef ecosystem.
CONNECTIVITY OF BIVALVES BETWEEN MANGROVE AND SEAGRASS ECOSYSTEMS IN KELAPA DUA ISLANDS: KONEKTIVITAS BIVALVIA ANTARA EKOSISTEM MANGROVE DAN LAMUN DI PULAU KELAPA DUA Endang Sunarwati Srimariana; Azizah Lutfia Ningtyas; Dea Fauzia Lestari; Beginer Subhan; Neviaty Putri Zamani; Tumpak Sidabutar
Jurnal Teknologi Perikanan dan Kelautan Vol. 16 No. 2 (2025): MAY 2025
Publisher : Fakultas Perikanan dan Ilmu Kelautan, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24319/jtpk.16.198-211

Abstract

Pulau Kelapa Dua, bagian dari Kepulauan Seribu, kaya akan keanekaragaman hayati dalam ekosistem mangrove dan lamun. Bivalvia berfungsi sebagai bioindikator yang penting untuk menilai kesehatan ekosistem ini. Penelitian ini bertujuan untuk menganalisis perbedaan keanekaragaman bivalvia antara lingkungan mangrove dan lamun dan mempelajari hubungan bivalvia pada kedua habitat ini. Data dikumpulkan melalui survei kepadatan mangrove dan lamun, pengukuran kualitas air, dan analisis sedimen. Metode transek garis dan plot digunakan untuk pengamatan mangrove, sedangkan metode Seagrass Watch diterapkan untuk pengamatan lamun. Sampel bivalvia dikumpulkan menggunakan pipa PVC (hingga kedalaman 10 cm) untuk individu di bawah permukaan dan survei visual untuk spesies yang terlihat di permukaan. Substrat berpasir di pulau ini mendukung berbagai spesies bivalvia. Sebanyak lima belas spesies bivalvia dari enam famili teridentifikasi: Tellinidae, Cardiidae, Veneridae, Lucinidae, Donacidae, dan Pinnidae. Kepadatan bivalvia secara signifikan lebih tinggi di ekosistem lamun (1.029 ind./100 m²) dibandingkan dengan ekosistem mangrove (366 ind./100 m²), dengan famili Tellinidae menunjukkan kepadatan tertinggi (664 ind./100 m²). Indeks ekologi menunjukkan kondisi yang stabil, dengan komunitas bivalvia yang beragam dan tersebar merata, mencerminkan lingkungan yang sehat. Analisis korespondensi mengungkapkan bahwa spesies bivalvia lebih terkonsentrasi di stasiun yang merepresentasikan ekosistem lamun, menegaskan hubungan yang lebih erat dengan habitat lamun.
Identification of Dietary Preferences in Groupers from Raja Ampat Reefs Through Mitochondrial DNA Metabarcoding Haqqy Rerian Erlangga; Raissha Amanda Siregar; Nurlita Putri Anggraini; Ester Restiana Endang Gelis; Erin Borbee; Christopher E. Lane; Inna Puspa Ayu; Beginer Subhan; Hawis Madduppa; Meutia Samira Ismet
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 30, No 4 (2025): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.30.4.507-518

Abstract

Groupers (Family Serranidae) are well known as top predators in the coral reef ecosystem. This family is one of the most important ecological organisms in the food web at Raja Ampat reefs. The aim of this study, therefore, was to investigate the food composition as well as the inter and intra-specific competition of two Serranidae species, including Epinephelus malabaricus and Epinephelus areolatus in Waisai, Raja Ampat. This study is expected to fill gaps on groupers food preferences. A total sample were collected at the fish landing site was 6 fish (2 sample of Epinephelus areolatus and 4 sample of Epinephelus malabacrius). DNA Amplification was performed using the COI mithocondrial gene marker (HCOI12198 and LCOI1490 primers). Bioinformatics and data analysis using QIIME 2 and R Software. This further comprised of α and β diversities. The results showed that Serranidae diet composition comprise of 13 species. Epinephelus malabaricus species demonstrated more diet composition varieties than Epinephelus areolatus. The most abundant diet found in all fishes’ gut samples was come from Scianidae family. The results indicate that there is an absence of any interspecific competition between the two species studied, due to a wide variation of diet composition. Conversely, intraspesific competitions existed amongst the Epinephelus malabaricus niches. This study highlighted DNA Metabarcoding application in trophic level and food web studies, as well as facilitating the development of information for ecological references.
Co-Authors Abdul Ajiz Siolimbona Abdul Ajiz Siolimbona Abdul Motalib Angkotasan ABDURRACHMAN BAKSIR Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir, Abdurrachman Abjan Ibrahim, Abjan ACHIS M. SIREGAR Adewal, Murad Alvian K ADITYA BRAHMANDITO Aditya Bramandito ADITYA BRAMANDITO ADITYA BRAMANDITO ADRIANI ADRIANI Adriani Sunuddin Adriani Sunuddin AFIFAH F. JANSIT Afiff , Usamah Agus Ramli Ahmad Eko Suprianto Ahmad Taufik Ghozali Ahmad Taufik Ghozali AHMAD TAUFIK GHOZALI Aisyah, Siti Zanuba Aji Wahyu Anggoro AJI WAHYU ANGGORO Alan Frendy Koropitan Alfaiz Arselan ALFAIZ ARSELAN Ali Mashar Aliati Iswantari Alimuddin Alla Asmara AM AZBAS TAURUSMAN Am Azbas Taurusman Andhika Prima Prasetyo ANDIKA NOVRIANSYAH Andrianus Sembiring ANDRIANUS SEMBIRING ANDRIANUS SEMBIRING Anggi Mayulina Daulay Anggraini, Nurlita ANIK MARTINAH Anwar, Muhammad Fathur Aradea Bujana Kusuma Aradea Bujana Kusuma Ardan Samman Arief Pratomo Arif Miftahul Aziz, Arif Miftahul ARINI S. P. PUTRI Ario Damar Arta Silaban Asadatun Abdullah Asep Sandra Budiman Ayu Ervinia Ayu, Inna Puspa Aziz, Nasrul Azizah Lutfia Ningtyas Bashari, Muhammad Hasan Benyamin, Azhari Bertoka Fajar S.P. Negara Borbee, Erin Budi Prabowo BUDIONO SENEN Carolline Desrainy Nababan Christopher E. Lane Citra S. U. Dewi Citra Satrya CITRA SATRYA UTAMA DEWI Citra Satrya Utama Dewi Dea Fauzia Lestari, Dea Fauzia Dedi Soedharma Dedi Soedharma Denny Khaerudi DENNY KHAERUDI DENNY KHAIRUDI DERTA PRABUNING DEWA GEDE RAKA WIADNYA Dewi, Citra Satrya Utama Diah Ayuningrum, Diah Dian Pertiwi Dietrich Geoffrey Bengen Dietrich Geoffrey Bengen Dietriech G Bengen DIETRIECH G. BENGEN DIETRIECH GEOFFREY BENGEN DIETRIECH GEOFFREY BENGEN Dietriech Geoffrey Bengen Dietriech Geoffrey Bengen DONDY ARAFAT Dondy Arafat Dondy Arafat DONDY ARAFAT Dondy Arafat DONDY ARAFAT Dondy Arafat Dondy Arafat DONDY ARAFAT Dondy Arafat DOUGLAS FENNER DWI LISTYO RAHAYU Dwi Yuni Wulandari E Paembonan, Rustam EDWIN JEFRI Efahmi, Efahmi EFRIN ANTONIA DOLLU EKA MAYA KURNIASIH Eko S Wibowo Eko S Wibowo Elfahmi Elfahmi, Elfahmi ELOK FAIQOH Elok Faiqoh ENDANG S. SRIMARIANA Endang Sunarwati Srimariana Enny Rimita Sembiring ENY SUPARYANI Erin Borbee Ester Restiana Endang Gelis Fadel Muhammad Fadhilah Rahmwawati FADHILLAH RAHMAWATI Fadillah Rahmawati Fahlevi, Arief Reza Fajar Maulana Fauzan Dzulfannazhir Fauzan Dzulfannazhir Febriantika, Putri Fenetiruma, Syamsudin FENI IRANAWATI Firdaut Ismail FIRDAUT ISMAIL Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail GATOT CIPTADI Gelis, Ester R. E. Genadi Algadri GESTEN HAZERI GIYANTO GIYANTO GIYANTO GIYANTO Hagus Tarno Haqqy Rerian Erlangga Hardianti, Aprilia Hari, Restu Fajar Hawis MADDUPPA HAWIS MADDUPPA Hawis H Madduppa HAWIS H MADDUPPA HAWIS H MADDUPPA HAWIS H. MADDUPPA HAWIS H. MADDUPPA HAWIS H. MADDUPPA HAWIS MADDUPPA Hawis Madduppa Hawis Madduppa Hawis Madduppa HEDI INDRA JANUAR HERDIAN WICAKSONO Herettrenggi, Rosa Hermansyah Amir, Hermansyah Husna, Alief K I WAYAN NURJAYA I Wayan Nurjaya Idham A. Nasrullah Ikbal Marus Ikbal Marus Ikbal Marus, Ikbal Ilham, Yuwanda INDRA PUTRA ALMANAR INNA PUSPA Irianda, Nadya Jeny Irmalita Tahir Irmalita Tahir Irmalita Tahir, Irmalita Irnin Agustina Dwi Astuti Islami, Nurfara Ismail, Firdaut Iswandi Wahab Iswandi Wahab Jansit, Afifah F. Jeddah Isnul Jelita Rahma Hidayati Jhoni Wahyu Adi Joko Santoso Junaedy, Ahmad Kahar, Fauzi Abdul Karizma Fahlevy Kesha Mufthi Java Tanaya Khairudi, Denny Kintani, Novia Indah KUNCORO, IWAN - KUSTIARIYAH TARMAN Kusumarani, Dheni L.M. IQBAL MUKSIN LALANG LALANG Lalu M Iqbal Sani LALU M. IQBAL SANI Lalu M. Iqbal Sani Lalu M. Iqbal Sani Lane, Christopher LUDI PARWADANI AJI LUDI PARWADANI AJI Luzmi Malia Izza M. Janib Achmad Maestro Munru Mai Efdi Majariana Krisanti Malaihollo, Jantje Jacobus Manafi, Efi Noferya MAREIKE HUHN Mas Irfanto Masyerli, Scify Bilqis Nawafi Meutia Samira Ismet Meutia Samira Ismet Minsaris, La Ode Alam Miswandi, Muhammad Mochamad Untung Kurnia Agung Mohamad Gazali Mohammad Mukhlis Kamal Muhamad Gilang Arindra Putra Muhamad Gilang Arindra Putra Muhamad Iqbal MUHAMMAD ABRAR MUHAMMAD ABRAR Muhammad Iqbal Muhammad Irfan Muhammad Izzat Nugraha Muhammad Janib Achmad Muhammad Taufik Muhammad Yusuf Muhandis Shidqi Munru, Maestro MUTIARA KRISTINA MARGARETHA Muttaqin, Ahmad Sabilul Mu’min Mu’min N Natih, Nyoman Metta N, Furqan Nadya Cakasana Nadya Jeny Irianda Najamuddin Najamuddin Najamuddin Najamuddin, Najamuddin Nasrul Aziz Nebuchad Nezzar Akbar Nebuchadnezzar Akbar Nebuchadnezzar Akbar NEVIATY P. ZAMANI Neviaty P. Zamani NEVIATY P. ZAMANI NEVIATY PUTRI ZAMANI NEVIATY PUTRI ZAMANI NEVIATY PUTRI ZAMANI NEVIATY PUTRI ZAMANI Neviaty Putri Zamani Neviaty Putri Zamani NI KADEK DITA CAHYANI Ni Kadek Dita Cahyani, Ni Kadek Dita NI WAYAN PURANAMASARI NI WAYAN PURNAMA SARI Niken TM Pratiwi Nor Jannah, Nor Novriyandi Hanif Nur Ari Bayu Nurlisa Alias Butet Nurlita Anggraini NURLITA P ANGGRAINI Nurlita Putri Anggraini NURLITA PUTRI ANGGRAINI Nurlita Putri Anggraini Nurlita Putri Anggraini Nurlita Putri Anggraini NURLITA PUTRI ANGGRAINI Nyoman M N Natih Nyoman Metta N Natih Nyoman Metta N Natih NYOMAN METTA N. NATIH P. JOANA DIAS Paembonan, Rustam E Prakas Santoso Prakas Santoso Prakas Santoso Prakas Santoso Prehadi . PREHADI PREHADI Prehadi Prehadi Prehadi, Prehadi Purwanto, Budi Rachma ati, Rita Radixa Radhya Ramadhan Haryanto RADIXA RADYA RAMADHAN HARYANTO Rahamadya, Aldiano Rahimah, Insaniah RAHMAD RAHMAD Rahman Rahman Rahmat Hidayat Rahmat, Fadhlan Basiluddin Raismin Kotta Raissha Amanda Siregar Ramadian Bachtiar Ramili, Yunita Rani Utari Ayuningtyas Rasidi Rasidi Raunsai, Jhoni Elias Raweyai, Oktofianus Obaja Restu, Yunan Gilang Ridha Fathihatunnisa RIDHO Z. WICAKSONO RIKOH MANOGAR SIRINGORINGO RIKOH MANOGAR SIRINGORINGO RISANDI DWIRAMA PUTRA RISANDI DWIRAMA PUTRA RISKI FADILLAH RITA RACHMAWATI Rita Andini RITA ANDINI Rita Andini Riyadi Subur Riyadi Subur, Riyadi Rommy M. Abdullah Rotinsulu, Dordia Anindita Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam Effendi Paembonan Rustam Effendi Paembonan, Rustam Effendi Ryan Adhitia Muslim Safika S, Safika Salim Abubakar Samira Ismet, Meutia Samsuardi Samsuardi, Samsuardi Sani, L. Mukhsin Iqbal Sani, Lalu M Iqbal Sani, Lalu M. Iqbal Sani, Lalu Mukhsin Iqbal Santoso Budi Widiarto Satya Pratama Atmaja Sebastian C. A. Ferse Sebastian Ferse SEKAR MIRA HERANDARUDEWI Setiamarga, Davin H. E. Siolimbona, Abdul Ajiz SITI KHODIJAH Siti Maysaroh Siti Maysaroh SLAMET WAHYUDI Sombo, Hendrik SUKANDAR SUKANDAR Sukandar Sukandar Sukandar, Mr. SUKMARAHARJA A. TARIGAN Syafrizayanti, Syafrizayanti Syahid SYAMSUL BAHRI AGUS Syamsul Bahri Agus, Syamsul Bahri TRI PRARTONO TRI PRARTONO Tumpak Sidabutar Ulandari, Rafika Wahab, Iswandi Wedi Andika Wibowo, Eko S Wibowo, Eko Setyobudi Wiwid Andriyani Lestariningsih Yahya, Rodhiyah Yennafri, Yennafri Yidoatimojo, Sudibyo Yoga Budi Bhakti yoman Metta N Natih Yosie Andriani Yudha, Firsta Kusuma Yuliana Fitri Syamsuni Yusli Wardiatno Zamani, Nevyati P. Zedta, Raymon Rahmanov ZULFANDRA BACHRUL KALAM Zulham Apandy Harahap Zulhan Arifin Harahap