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All Journal PROSIDING SEMINAR NASIONAL Jurnal Penelitian Saintek Indonesian Journal of Cancer Chemoprevention Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Biomedika Jurnal Kesehatan Pharmacon Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Journal of Nutraceuticals and Herbal Medicine Martabe : Jurnal Pengabdian Kepada Masyarakat Indonesian Community Journal Jurnal Farmasi Sains dan Praktis Proceeding ISETH (International Summit on Science, Technology, and Humanity) Prosiding University Research Colloquium Jurnal Farmasi Klinik dan Sains Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Kefarmasian Indonesia Pharmacon: Jurnal Farmasi Indonesia
Andi Suhendi
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Pengawasan Mutu Produk Obat Herbal Berbasis Curcuma sp. Dengan Parameter Kadar Kurkumin Menggunakan Metode Kromatografi Cair Kinerja Tinggi Hanwar, Dedi; Anwar, 'Aina Fahrina; Suhendi, Andi
Prosiding University Research Colloquium Proceeding of The 13th University Research Colloquium 2021: Kesehatan dan MIPA
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Pengawasan terhadap produk obat herbal berbasis Curcuma sp. dilakukan dengan tujuan untuk menjamin mutu yang berhubungan dengan keamanan, toksisitas, dan hukum. Kurkumin merupakan komponen senyawa kurkuminoid yang memiliki persentase paling besar dibandingkan bisdemetoksikurkumin maupun demetoksikurkumin dengan beragam khasiat. Penelitian ini dilakukan untuk mengembangkan kontrol mutu produk obat herbal berbasis kurkumin dengan menggunakan Kromatografi Cair Kinerja Tinggi (KCKT). Sistem kromatografi menggunakan kolom C18 5 ?m 4,6x150 mm, detektor PDA dengan panjang gelombang 425 nm, fase terbalik dan sistem elusi isokratik menggunakan fase gerak asetonitril : asam fosfat 0,5% (60 : 40) dengan laju alir 0,8 mL/menit. Dari dua produk yang diteliti diperoleh kadar setiap kapsulnya adalah 4,92 mg (% recovery 80, 100, dan 120%: 127,30±3,21%, 132,40±1,77%, 120,36±12,36%; RSD keterulangan 1,55%; dan RSD presisi antara 1,27%) untuk produk I. Sedangkan untuk produk II adalah 1,90 mg (104,58±4,68%, 108,08±0,20%, 98,89±1,28%; 0,66%; dan 1,16%). Uji keseragaman kandungan untuk produk I memenuhi kriteria 4,996 mg (99,91% dalam rentang 85,0%-115,0%) sedangkan produk II tidak dilakukan uji yang sama.
Aktivitas Penghambatan Enzim ?-Glucosidase Ekstrak Daun Zingiber zerumbet Suhendi, Andi; Hanwar, Dedi; Santoso, Broto; Abidi, Subhan R
Prosiding University Research Colloquium Proceeding of The 16th University Research Colloquium 2022: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Penelitian sebelumnya menyatakan bahwa senyawa zerumbon dan kaempferol dari Zingiber zerumbet dan ekstraknya memiliki aktivitas penghambatan enzim ?- glukosidase. Oleh karena itu, daun zingiber zerumbet diduga memiliki aktivitas sebagai penghambat enzim ?-glukosidase. Penelitian ini bertujuan untuk mengetahui aktivitas penghambatan enzim ?-glukosidase dan mekanisme penghambatannya. P-nitrofenol yang terbentuk dari reaksi antara enzim dengan p-nitrofenil-?-D-glukopiranosa (PNPG) diukur dengan microplate reader pada panjang gelombang 405 nm. Penghambatan enzim dilakukan dengan menggunakan berbagai konsentrasi ekstrak dan acarbose sebagai kontrol positif. Uji kinetika enzim menggunakan 4 seri konsentrasi substrat pnitrofenil- ?-D-glucopyranose (PNPG). Hasil penelitian menunjukkan bahwa ekstrak etanol daun Zingiber zerumbet memiliki aktivitas penghambatan enzim ?-glukosidase dengan nilai IC50 sebesar 22,21±0,20 ?g/mL. Sedangkan nilai IC50 acarbose adalah 47,41±2,37 ?g/mL. Aktivitas penghambatan ekstrak lebih baik dibandingkan acarbose. Mekanisme penghambatan merupakan tipe camnpuran karena nilai Kap>K dan Vap<V.
Pengobatan Gratis Sebagai Upaya Meningkatkan Kesehatan Masyarakat Desa Potronayan, Kecamatan Nogosari, Kabupaten Boyolali Karuniawati, Hidayah; Haryoto, H; Sujono, Tanti Azizah; Fortuna, Tista Ayu; Khotimah, Khusnul; Suhendi, Andi; Ichsan, Burhanudin; Sarbini, Dwi; Muhtadi, M; Harlianti, Mariska Sri; Dewi, Listiana Masyita; Herawati, Erna; Studiviani, Raafika; Utama, Haya Nabilah; HI, Marsilia Rosa Sinensis; Dzafira, Laila; Nurseptiria, Haliza Arzeti; Oktaviani, Ardalia Rinanda; Fitriani, Rizki Ainun; Kusumastuti, Lina Ayu; Muslihah, Sayyidah Nurul; Anifatussaa'dah, A; Nisa', Faridita khoirun; Trihatmoko, Fahriza Mei; Basusena, Rahardian Surya; Rifaini, Fidhia Nur; Citra, Tia Mella; Maesaroh, Ida; Marsya, Vitania
Prosiding University Research Colloquium Proceeding of The 16th University Research Colloquium 2022: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Kesehatan merupakan salah satu kebutuhan penting setiap orang karena dengan tercapaianya kesehatan akan memungkinakan terlaksananya kegiatan atau aktifitas yang lain. Kesehatan merupakan hak setiap orang dengan tidak mengenal tingkat ekonomi seseorang. Ekonomi yang minim diprediksi menjadi salah satu hambatan dalam mendapatkan kesehatan yang maksimal. Peran tenaga kesehatan sangat diperlukan dalam mambantu pasien mendapatkan kesehatan yang optimal. Salah satu kegiatan yang dapat membantu masyarakat terutama dengan ekonomi yang minim adalah pengobatan gratis. Pengobatan gratis yang merupakan salah satu kegiatan pengabdian kepada masyarakat telah dilakukan di balai desa Potronayan kecamatan Nogosari, kabupaten Boyolali pada ahad 31 Juli 2022. Selain pengobatan gratis, kegiatan yang lain adalah pemeriksaan tanda-tanda vital, pelayanan obat dan konseling obat serta pelayanan gizi. Kegiatan ini diikuti oleh 105 pasien. Pasien kebanyakan adalah wanita (73,3%) dengan usia terbanyak adalah antara 51 tahun sampai 60 tahun sebanyak 32,38%.
PEMBERDAYAAN KELOMPOK WANITA TANI PRODUSEN HERBAL HEALTHY DRINK BERBASIS ONLINE E- COMMERCE DI DESA BINAAN POTRONAYAN, BOYOLALI Haryoto, H; Karuniawati, Hidayah; Suhendi, Andi; Santoso, Broto; Fortuna, Tista Ayu; Praswati, Aflit Nuryulia; Dewi, Triana Ariska; Keisya, Aulita; Auliya, Dhiyahul; Ahmad, Farhand
Prosiding University Research Colloquium Proceeding of The 19th University Research Colloquium 2024: Bidang Pengabdian Masyarakat
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Kabupaten Boyolali memiliki 19 kecamatan. Masing-masing kecamatan menghasilkan komoditas tanaman pangan yang berbeda beda. Salah satu komoditas yang dihasilkan di Kabupaten Boyolali adalah komoditas padi-padian, sayuran, dan empon-empon. Salah satu daerah penghasil empon-empon adalah desa Potronayan. Desa Potronayan merupakan desa binaan UMS yang terletak di pinggir Boyolali sebelah utara bandara Adisumarmo dengan jumlah penduduk sekitar 4.984 jiwa yang mayoritas penduduknya berpenghasilan dari bercocok tanam, salah satunya adalah tanaman yang merupakan jenis obat herbal seperti jahe, temulawak, kunyit, dan kencur. Rimpang (empon-empon) ini banyak digunakan sebagai ramuan obat tradisional dan sebagai bumbu dalam masakan. Selain itu empon-empon juga banyak digunakan untuk pengobatan maupun pencegahan gangguan kesehatan. Kegiatan ini bertujuan untuk (1). Meningkatkan harga jual empon-empon, (2). Membuat diversifikasi produk berupa herbal healthy drink, (3). Mempromosikan produk menggunakan platform online E-Commerce yang dapat diakses melalui internet. Kegiatan ini juga merancang dan mengaplikasikan green economy. Green economy meningkatkan kesejahteraan manusia dan kesetaraan sosial, sekaligus mengurangi risiko lingkungan secara signifikan. Hasil Kelompok Wanita tani berupa rimpang yang berkhasiat tersebut sebelum dijual bisa diolah menjadi suatu produk herbal healthy drink yang mempunyai daya jual lebih tinggi daripada bahan mentahnya.
Reducing Activity of Interleukin-8 (IL-8) Extract of Sea Cucumber (Holothuroidea sp.) in Experimental Animals Induced by Carrageenan Aurum, Mursalina Nanda; Suhendi, Andi; Da'i, Muhammad; Fauzi, Ahmad
Pharmacon: Jurnal Farmasi Indonesia Vol 21, Special Issue 1, 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v21i0.23471

Abstract

Interleukin-8 is a pro-inflammatory cytokine that plays a role in the inflammatory response. Sea cucumbers (Holothuria sp.) are used as alternative medicines that have anti-inflammatory activity. The study aims to determination of Sea cucumber (Holothuria sp.) as anti inflammation activty based on capability of reducing interleukin-8 (IL-8) on Rats induced by Carrageenan. Experimental research used was a post-test-only control group design. A total of 25 rats were divided into 5 groups, namely negative control, positive control (Na. Diclofenac), and treatment groups given Sea cucumber extract (doses of 45, 90, and 180 mg/ g BW). Carrageenan-induced mouse paw edema model was used for induction of inflammation. IL-8 of Paw tissue were measured by an ELISA reader at 450 nm wavelength. The result showed that treatrment groups has anti-inflammation activity based on IL-8 levels compared to negative and positive controls. The lowest levels of IL-8 showed at a dose of 90 mg/g BW. Based on One-way ANOVA, there were no significant differences of between groups. Sea cucumber has anti-inflammation activity by reducing IL-8 at all dose tested
Validation of Analytical Method LC MS/MS for Determination Isoniazid in Rats Serum Suhendi, Andi; Rohman, Abdul; Wahyono, Djoko; Nurrochmad, Arief; Manggo, Tasya Faradillah
Pharmacon: Jurnal Farmasi Indonesia Vol 20, No 2 (2023)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v20i2.23293

Abstract

Determination of isoniazid concentration for therapeutic drug monitoring must produce valid values, so validation of the analytical method of LC-MS/MS needs to be carried out. This research aims to obtain valid method of LCMS/MS in order to determination isoniazid in rats serum. Sample preparation was carried out using the liquid-liquid extraction method using acetonitrile and methanol (2:1). The chromatography system was developed with a C18 column (ACQUITY UPLC® HSS T3 1.8 µm, 2.1 × 100 mm), the mobile phase was 70% acetonitrile with 0.1% formic acid (A), water with 0.1% formic acid (B), as well as methanol (C) with a flow rate of 0.3 mL/min, with gradient elution, detection in ESI+ mode with multiple reaction monitoring (MRM) at the transition 138 m/z → 121 m/z. The research results showed that the method was accurate with a % recovery of 96 - 113% and an RSD of 2.4 ± 0.9%. The precision value was obtained with an average RSD of 3.7 ± 2.4% for intra-day and 4.1 ± 2.3% for inter-day. Linearity showed good results with a correlation coefficient of 0.9956 in tests with 5 different concentrations. The LOQ and LOD values obtained from the linear regression equation were 0.1987 µg/mL and 0.0656 µg/mL respectively. The results showed that the LC-M/MS method was valid for determining isoniazid levels in rat serum
Selectivity Index of Alpinia galanga Extract and 1’-Acetoxychavicol Acetate on Cancer Cell Lines Da'i, Muhammad; Meilinasary, Khairunnisa Azani; Suhendi, Andi; Haryanti, Sari
Indonesian Journal of Cancer Chemoprevention Vol 10, No 2 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss2pp95-100

Abstract

Previous research stated that galangal (Alpinia galanga) extract has a potential as cytotoxic agent with active compound of 1’-Acetoxychavicol Acetate (ACA). The objective of this study was to determine the selectivity of ethanol extract, ethyl acetate fraction, and methanol fraction of of galangal, and ACA on cancer cell lines. Cytotoxic activity was carried out using the MTT method on T47D breast cancer, WiDr colon cancer, HeLa cervical cancer, and Vero normal cell lines. The results showed that galangal ethanol extract and its fractions had selectivity index equal to or less than 2 on cancer cells. Meanwhile, ACA had selectivity index more than 3 on T47D cell and HeLa cell. ACA showed a strong cytotoxic activity against cancer cells T47D, HeLa, and WiDr with IC50 values of 3.14, 7.26, and 12.49 μg/ml, respectively. Based on data, it could be concluded that ACA was the most selective to inhibit T47D cell with a selectivity index of 6.6.Keywords: 1’-Acetoxychavicol acetate, galangal (Alpinia galanga), selective index, cytotoxic
PENETAPAN KADAR PROTEIN PRODUK SUSU BUBUK DENGAN METODE KJELDAHL Fauzi, Ahmad; Suhendi, Andi
Jurnal Farmasi Klinik dan Sains Vol 3, No 2 (2023): Jurnal Farmasi Klinik dan Sains
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Gombong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jfks.v3i2.1269

Abstract

Protein merupakan senyawa organik kompleks dengan bobot molekul besar dan sebagai sumber nutrisi yang paling baik untuk pertumbuhan. Susu adalah salah satu hasil ternak yang dikenal sebagai bahan makanan bernilai gizi tinggi dan sering dikonsumsi untuk memenuhi kebutuhan protein tubuh salah satunya ialah protein. Metode kjeldahl digunakan untuk menganalisis kadar protein kasar dalam bahan makanan secara tidak langsung, misalnya pada asam amino, protein dan senyawa lain yang mengandung nitrogen, karena yang dianalisis dengan cara ini adalah kadar nitrogennya. Analisis Kualitatif yang telah dilakukan pada sampel susu bubuk merek X didapatkan hasil positif mengundung protein dengan timbul-nya warna ungu atau biru violet akibat pereaksi biuret yang bereaksi dengan gugus amida asam bersama gugus lainnya. Analisis kualitatif dilakukan menggunakan metode metode Kjedahl, dapat disimpulkan bahwa sampel mengandung protein 4,56 % + 0,1% mendekati klaim produk 5%.
The Potency of Kitolod (Isotoma longiflora (L)Presl.) Herb Extract as a Cure for Cervical Cancer: an in Vitro Study of Hela Cells Hapsari, Atikah; Asti, Dhinar; Selviana, S; Hidayati, Ria; Kumalla, Novea; Suhendi, Andi
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2016: Proceeding ISETH (International Conference on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.2359

Abstract

Cervical cancer is the leading cause of death in women in the world. Cases of cervical cancer are the majority causes of death occurring in developing countries. The existing medicines which have a lot of functions as anticancer, however, still can damage the normal cells in our body. Cancer treatment using chemotherapy also has some side effects. The condition encourages researchers to explore anticancer cure made of natural ingredient. This research was conducted to find the potency of ethanol extract of herb kitolod (Isotoma longiflora (L) Presl.) as a cervical cancer cure to heLa cell. The method being used for the cytotoxic test on HeLa cells was MTT assay. Absorbant performed by ELISA reader at a wavelength of 595nm. Phytochemical screening which had been conducted showed a significant result that kitolod plants contain secondary metabolites compounds such as flavonoids, steroids, saponins, tannins and alkaloids. The results of cytotoxic test of the kitolod herb ethanol extract on HeLa cells by using MTT assay method acquired a value of IC50 of 227 μg/mL. This study showed that the ethanol extract of the herb kitolod had the potency to inhibit cervical cancer cells (HeLa cells) in a moderate ability.
Potential of Snakehead Fish and Sea Cucumber Extracts on Increase Anticancer Drugs Potency through Inhibition of Glutathione S-Transferase Suhendi, Andi; Putri, Ega Annisa; Tasysaningtyas, Thalia Fiandra; Abdillah, Safira Mafaza; Aisy, Inandrohatul; Utami, Wahyu
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 2, AUGUST 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

In certain conditions of cancer, isoenzymes of glutathione-S-Transferase (GST) showed increased activity. Consequently, anticancer drugs would be inactive due to increase of phase II metabolism through conjugation with glutathione. In order to increase the effectiveness of anti-cancer drugs, a GST inhibitor is required. One of the natural sources have antioxidant activity is snakehead fish. The study aims to determine GST inhibition activity of the combination of extract of snakehead fish (SF) and sea cucumber (SC). This activity was determined using rat liver and lung microsomal-cytosolic fractions obtained through the Lundgren method. GST activity was measured spectrophotometrically using a simple kinetic program at a wavelength of 345 nm with a DCNB (1.2-dichloro-4-nitrobenzene) as a substrate. The results showed an increase in the percentage of inhibition GST enzymes of the liver and lungs of rats by extracts and their combinations. The data of IC50 values showed that the smallest value for liver GST was found in SC with the value of 117.7 µg/mL and for lung GST enzymes, it was a combination of SC-SF (1:2) with a value of 45.6 µg/mL. This showed that these two samples had the greatest activity in inhibiting lung and liver GST enzymes and the potential as a complementary therapy for cancer patients.
Co-Authors 'Aina Fahrina Anwar . Haryoto . Haryoto, . . MUHTADI . Muhtadi A Anifatussaa'dah Abdillah, Safira Mafaza Abdul Rohman Abidi, Subhan R Aflit Nuryulia Praswati Agus Triyono Agus Ulinuha Ahmad, Farhand Ainur Tri Hapsari Aisy, Inandrohatul Alida Rahmalia Damayanti Alvita Marshanda Purnama Puteri Amalinda Ayu Faroska Anifatussaa'dah, A Annissa, Yanis Nur Anwar, 'Aina Fahrina Ardalia Rinanda Oktaviani Arief Nurrochmad Arifah Sri Wahyuni Asti, Dhinar Aulia Tassya Fadhilla Auliya, Dhiyahul Aurum, Mursalina Nanda Basusena, Rahardian Surya Broto Santoso Burhanudin Ichsan Cahyaningrum, Safira Citra, Tia Mella Citradewi, Lia Intan Da'i, Muhammad Dedi Hanwar Dewi, Triana Ariska Didik Setiawan Djoko Wahyono Dwi Lestari Dwi Sarbini Dzafira, Laila Eka Prasnaparamita W Eky Wahyu Novarista Em Sutrisna Erindyah Retno Wikantyasning Erlya Ayusari Ramly Erna Herawati Erna Herawati, Erna Fadhilla, Aulia Tassya Fahriza Mei Trihatmoko Farah Salsabila Farid Adi Prasetya Faridita khoirun Nisa' Faroska, Amalinda Ayu Fauzi Ahmad Muda Fidhia Nur Rifaini Fitriani, Rizki Ainun Fortuna, Tista Ayu Frida Rosenova Gunawan Setyadi Hadi, Ismanurrahman Haliza Arzeti Nurseptiria Hamimatusalma Alkautsar Hamzah, Hasyrul Handayani, Vivi Resty Hapsari, Atikah Haryoto - Haryoto Haryoto Haryoto, H Haya Nabilah Utama Heni Lutfiyati, Heni Hesti Pawarti HI, Marsilia Rosa Sinensis Hidajah Rachmawati Hidayah Karuniawati Hidayati, Ria Husna Amalia Halwa Ichsan, Burhanudin Ida Maesaroh Ida Maesaroh, Ida Ika Trisharyanti Dian Kusumowati Jessica Rosalinda Joko Priyanto Wibowo Kasful Asra Sakika Keisya, Aulita Khairunnisa Azani Meilinasary, Khairunnisa Azani Kharisma Aulia Husna Khusnul Khotimah khusnul khotimah Kumalla, Novea Kusumastuti, Lina Ayu Laila Dzafira Lia Intan Citradewi Lina Ayu Kusumastuti Listiana Masyita Dewi M Muhtad Manggo, Tasya Faradillah Mariska Sri Harlianti Marsilia Rosa Sinensis HI Marsya, Vitania MAULANA, RIZA Muhammad Da&#039;i Muhtad, M Muhtadi Muhtadi - Muhtadi Muhtadi Muhtadi, M Muslihah, Sayyidah Nurul Nida’ Nur Jannah Nisa', Faridita khoirun Nur Muhammad Arrizky Nurcahyanti Wahyuningtyas Nurcahyanti Wahyuningtyas, Nurcahyanti Nurseptiria, Haliza Arzeti Nurul Tri Anggraini Oktaviani, Ardalia Rinanda Pawarti, Hesti Pawarti, Hesti Peni Indaryudha Peni Indaryudha, Peni Puspa, Fajrina Eka Putri, Ega Annisa Raafika Studiviani Rahardian Surya Basusena Ranatri Puruhita Ria Utami, Primanitha Rifaini, Fidhia Nur Rizki Ainun Fitriani Rizky Ainun Fitriani Rosa Sayentina Amin Sabtanti Harimurti, Sabtanti Sari Haryanti Sayyidah Nurul Muslihah Selviana, S Siti Aisyah Siti Aisyah Studiviani, Raafika Subekti, Istiara Subhan R Abidi Tanti Azizah Sujono Tasysaningtyas, Thalia Fiandra Tia Mella Citra Tista Ayu Fortuna Trihatmoko, Fahriza Mei Utama, Haya Nabilah Vitania Marsya Vivi Resty Handayani Wahyu Utami, Wahyu Widyastuti, Vera Yanis Nur Annissa