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PENGARUH GLUTAMIN & GLUKOSA UNHIDRAT PADA JUMLAH LIMFOSIT TIKUS MODEL KURANG ENERGI PROTEIN Septa Katmawanti; Bambang Wirdjadmaji; Annis Catur Adi
Preventia : The Indonesian Journal of Public Health Vol 1, No 1 (2016)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.859 KB) | DOI: 10.17977/um044v1i1p25-34

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Abstract: Protein-energy malnutrition condition causes lymphocytes number to decrease which then lead to body's cellular immune system disorders, the condition where patient is more vulnerable to infection. Number of lymphocytes in the body is dependent to some factors, some of them are anti-oxidant, amino acid glutamine and glucose. The aim of his research is to study the effect of L-glutamine and glucose unhidrate administration in rat model of protein-energy malnutrition to the increase of lymphocytes number in the blood. This is an experimental study with Completely Random Design. Sample is malnutrition induced with parched rice for 15 days (n=20). Then some samples are examined to figure out the lymphocytes number after the rate model experience lack of energy and protein. Sample is then divided into four treatment groups, they are: group with normal diet (control), normal diet + L-glutamine, normal diet + glucose unhidrate, and normal diet + L-glutamine + glucose unhidrate. Lymphocytes content is measured again after 10 days. The result of the study shows that number of lymphocytes increase significantly (P<0.05) in the four treatment groups after the intervention compared with number of lymphocytes in the malnutrition induced group. Statistical analysis of the four groups with L-glutamine and glucose unhidrate administered shows significant difference each other (p<0.05). These observations imply that the glutamin and gluocose unhidrat has an effect to the increase of lymphocytes number in the blood.Keywords: Protein-Energy Malnutrition (PEM), lymphocyte, l-glutamine, glucose unhidrateAbstrak: Kondisi KEP menyebabkan penurunan jumlah limfosit yang mengakibatkan gangguan sistem imun seluler tubuh, sehingga penderita menjadi lebih rentan terhadap infeksi. Jumlah sel limfosit pada tubuh dipengaruhi oleh beberapa faktor antara lain keberadaan antioksidan, asam amino glutamin dan glukosa. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian glutamin dan glukosa unhidrat pada tikus model KEP terhadap kenaikan jumlah limfosit darah. Studi ekperimental menggunakan metode Rancang Acak Lengkap. Sampel diinduksi malnutrisi dengan nasi aking (karak) selama15 hari (n=20). Kemudian subsampel diperiksa untuk mengetahui jumlah limfosit setelah tikus mengalami kekurangan energi dan protein. Sampel kemudian dibagi menjadi empat kelompok perlakuan, yaitu kelompok dengan diet normal (kontrol), diet normal + glutamin, diet normal + glukosa unhidrat, dan diet normal + glutamin + glukosa unhidrat. Kadar limfosit diukur kembali setelah 10 hari. Hasil penelitian ini menunjukkan bahwa setelah dilakukan intervensi terdapat kenaikan jumlah limfosit secara bermakna (P<0,05) pada keempat kelompok perlakuan dibandingkan dengan jumlah limfosit pada kelompok induksi malnutrisi. Analisis secara statistik pada keempat kelompok yang diberikan intervensi Glutamin dan glukosa unhidrat menunjukkan bahwa terdapat perbedaan yang bermakna satu sama lain (p<0,05).Kata kunci: Kekurangan Energi Protein (KEP), limfosit, glutamin, glukosa unhidrat
Effect of Probiotic Milk containing L. casei Shirota on Immunoglobulin G Levels among Anemic Adolescent Girls Elya Sugianti; Annis Catur Adi; Ikeu Ekayanti; Trias Mahmudiono; Widati Fatmaningrum; Siti Nur Husnul Yusmiati
Health Notions Vol 2, No 2 (2018): February
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.919 KB) | DOI: 10.33846/hn.v2i2.138

Abstract

Probiotic play important role on immune systems. This study was to investigate effect of probiotic milk containing L. casei Shirota on immunoglobulin G levels among anemic adolescent girls. A total of 22 adolescent girls aged 13-18 years with anemia were included on this study. The subjects were separated into 2 groups i.e. a probiotic group and a control group by simple random sampling. During 4 weeks, a probiotic group received 1 tablet of 60 Fe elemental + 250 µg folate acid once a week and probiotic milk once daily. While a control group received 1 tablet of 60 Fe elemental + 250 µg folate acid once a week. Blood samples were collected at 0 and 4 weeks and assayed for immunoglobulin G levels by ELISA. Immunoglobulin G levels were not significantly increase after intervention. Compared with control group, probiotic milk administration in probiotic group did not significantly increased immunoglobulin G levels (p>0.05). The probiotic milk containing L. casei Shirota did not improve immunoglobulin G levels on anemic adolescent girls. Keywords: Probiotic, Immunoglobulin G levels, Anemia, Adolescent girls
The Effect of Green Coffee Bean Extract on The Weight Rats with High Fat Diet Arie Dwi Alristina; Annis Catur Adi; Rita Ismawati
Health Notions Vol 2, No 4 (2018): April
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.399 KB) | DOI: 10.33846/hn.v2i4.171

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WHO declares that obesity is a global epidemic. In 2014, 39% of adults >18 years old were overweight and 13% had obesity. This research aims to determine the effect of green coffee extract on the weight rats fed high-fat diet. Method: This research is true experimental pre-post test with control group design. The animals were grouped into 5 groups, i.e., group1 (negative control), group2 (positive controls fed high-fat diet), group3 (high-fat diet and GCBE dose of 100 mg/kg BW), group4 (high-fat diet and GCBE with dose of 200 mg/Kg BW), group5 (highfat diet and GCBE dose of 400 mg/Kg BW). The obtained data were tested with one-way ANOVA followed by LSD analysis with α=0.05. Results: This research proves that green coffee bean extract with a dose of 100, 200 and 400 mg/kg BW can lower weight of rats with higt fat diet. Discussion: Green coffee extract contains active compound of chlorogenic acid that can increase AMPK activity so it has positive impact that can direct the result of metabolite substance, which is useful in decreasing fat synthesis. The high fat oxidation process results in decreased fat synthesis characterized by burning fat into energy thereby decreasing fat cells in adipose tissue.Keywords: Obesity, Body weight, Chlorogenic acid, Green coffee
Involvement of Family Members and Nutrition Care Pattern of Positive Deviance Tri Ratnaningsih; Chatarina Umbul Wahyuni; Hari Basuki Notobroto; Annis Catur Adi
Health Notions Vol 2, No 4 (2018): April
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.583 KB) | DOI: 10.33846/hn.v2i4.176

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Nutritious food giving in under-fives to sustain the growth and development of under-fives optimally required the involvement of family members other than fathers. The purpose of this study was to determine the correlation between family members' involvement with the mother's nutrition care pattern of poor families in the Mojokerto regency of East Java. This research design was using case control with case population of poor mother with healthy under-five and control population was poor mother with nutritious problem child. Number of case group samples 79 people and control group 41 people. Sampling technique with multi stage sampling. Data collection using questionnaires that have been tested the validity and reliability to 30 people. Data analysis using chi square statistic test. The results showed that the involvement of other family members in the positive deviance mothers was good as much as 35 people (44.30%). In non-positive mother deviance involvement of most other family members were less than 31 people (75.61%), chi square obtained p = 0,000 and value α = 0,05, meaning there was correlation of family member involvement with care pattern of positive deviance mother. The involvement of family members motivated mothers to provide a good nutritional food to under-five children.Keywords: Positive deviance, Under five children, Care pattern, Nutrition
Type of Personality, Food Consumption and Physical Activity Levels as Determinants of Overweight and Obesity among Urban Adolescents Alfian Yusuf; Suryanto Suryanto; Annis Catur Adi; M. G. Bagus Ani Putra
Health Notions Vol 2, No 5 (2018): May
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.145 KB) | DOI: 10.33846/hn.v2i5.190

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Obesity is a global epidemic, it was health issues that must be addressed immediately. There was a very significant increase in the prevalence of overweight and obesity of adolescents. Several studies showed that social support related to eating disorders and physical activity of adolescents causes of overweight and obesity. Other study showed that personality contributes to health outcomes and it was associated with a controllable risk factor such as obesity. The aim of the study was to measure the relationship of personality type, fatty food consumption, and physical activity level to overweight and obesity among adolescents. We used a case-control design with 270 subjects. Case population was vocational or senior high school students with overweight or obesity and the control population was vocational or high school students without overweight or obesity. Data analysis used chi-square test with 95% confidence level (α = 0.05) and Odds Ratio (OR). The results showed that there was no significant relationship between personality type and overweight or obesity (p> 0.05). There was a significant relationship between the consumption of fatty foods (p = 0.001), the level of physical activity (p = 0.040) and overweight or obesity among adolescents. Keywords: Adolescents, Overweight, Obesity, Personality, Fatty foods, Physical activity
Influence of Additional Feeding Recovery on The Intellectual Intelligence of Children Mei Lestari Ika Widyyati; Irwanto Irwanto; Annis Catur Adi
Health Notions Vol 2, No 3 (2018): March
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.312 KB) | DOI: 10.33846/hn.v2i3.154

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Nutrition is the main requirement for a toddler, if not obtained food according to needs its nutrition value, it will interfere with the growing swell of current or next time. The purpose of this research was to analyze the effect of Additional Feeding Program Recovery on the intellectual intelligence children aged 4-5 years. Design of this research was observational cohort retrospective. The sample size was 70, were divided into two groups, group with a history of getting Additional Feeding Recovery and the Group didn't get Additional Feeding Recovery. Independent variable was Additional Feeding Recovery and the dependent variable was intellectual intelligence. Data was analyzed using Independent Sample t-test. There was no difference in the level of intelligence on the two group with p-value = 0.129. Kewords: Additional feeding recovery, Intellectual intelligence
The Difference of Results in Abdomen and Anus Area Measurement With Non-Contact Infrared Thermometer Anna Surgean Veterini; Nancy Margarita Rehatta; Hamzah Hamzah; Widijiati Widijiati; Sarmanu Sarmanu; Subijanto Marto Soedarmo; Widodo Jatim Pudjirahardjo; Annis Catur Adi; Heni Rachmawati; I Ketut Sudiana
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.022 KB) | DOI: 10.19087/jveteriner.2021.22.2.183

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Study about sepsis is chalenging to get the right therapeutic method. One clinical signs of the sepsis mice model is changes in body temperature. An easy way to examine body temperature is using infrared non-contact thermometer. The aim of this study is to compare the body temperature obtained from infrared non-contact thermometer at the abdomen and anus area. We used male Mus musculus mice, body weight of 25–30 grams, were divided into 2 groups (control and treated groups). In the control group, mice were injected with NaCl 0.9% solution, with the amount of NaCl volume equal to LPS. In the treated group, mice were injected with 2.5 mg/kgBW of LPS intraperitoneally. Body temperature measurement was measured in abdomen (tabd) and anus (tan) area. Body temperature was measured at 8th and 24th hour after LPS or NaCl injection. Body temperature value result using tabd was higher than tan. LPS injection to mice produced an increase in body temperature but was not significant when compared to the control group (8th and 24th hour). The mean difference between tabd and tan in 8th hour control groups respectively were 2.12oC. The mean difference between tabd and tan in 24th hour control groups 4.6oC. The mean difference in treated groups (8th hour) was 4.66oC, while it was 4.77oC in the 24th hour treated groups. LPS could caused vasodilation of the vessels, the measurement area of non contact-infrared thermometer will be the most important factor to be considered; tan may closer to the physiology condition.
PENGARUH SUBSTITUSI TEMPE DAN PENAMBAHAN ISOLATED SOY PROTEIN TERHADAP MUTU ORGANOLEPTIK DAN KANDUNGAN PROTEIN SOSIS AYAM Rizky Arifandy Amir; Annis Catur Adi
Media Gizi Indonesia Vol. 12 No. 1 (2017): JURNAL MEDIA GIZI INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.16 KB) | DOI: 10.20473/mgi.v12i1.80-87

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Chicken, soy tempeh, and Isolated Soy Protein (ISP) can be processed into sausages that can be alternative for highprotein snack. Red Yeast Rice is a natural food colorant added to that the sausage to make more interest. This research was aimed to determine the substitution effect of tempeh and addition of Isolated Soy Protein (ISP) to the organoleptic quality, protein value of chicken sausages. The type of research was pure experimental with completely randomized design. There was 3 types of formula, 1 control formula F0 (chicken) and 2 formula modifi cations F1 (50 g chicken, 50g tempeh, 15 g ISP, and 3 g of red yeast rice), F2 (100 g tempeh, 25 g ISP, 5 g red yeast rice ) with three replications. The organoleptic quality was determined by 35 untrained panelists. The results of organoleptic tests showed that F2 (color, taste, texture) was most preferred formulations. The highest protein content per 100 g sausage was F2 (17.4 g) compared with F0 (14.6 g). There were signifi cant differences between F0 to F1, F2 on fl avors (p = 0.000) and color (p = 0.000). The best formula rated by the composition, organoleptic test, and the protein content was F2.
DAYA TERIMA, KADAR PROTEIN DAN ZAT BESI COOKIES SUBTITUSI TEPUNG DAUN KELOR DAN TEPUNG KECAMBAH KEDELAI Yulia Kurnia Sari; Annis Catur Adi
Media Gizi Indonesia Vol. 12 No. 1 (2017): JURNAL MEDIA GIZI INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.603 KB) | DOI: 10.20473/mgi.v12i1.27-33

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Moringa leaves and soybean sprouts is local plant in Indonesia. Moringa leaves and soybeans sprouts have high enough of nutrient content but still less to utilization. Moringa leaves are rich in iron and soybeans sprouts as an alternative food material who source of vegetable protein can be substituted to cookies for supplementary food recovery in the rehabilitation phase. The purpose of this study was to analyze the effect of ubstitution of Moringa leaf powder and flour soybean sprouts against acceptance, the levels of protein, and iron cookies. Type of research was true experimental design with completely randomized design at 2 times replicating. Untrained panelists were used 30 mother ofchildren aged 1-3 years. Product acceptance was tested using statistical analysis Kruskal Wallis (α≤0,05). The result of product acceptance test was the most preferred panelist is cookies F1 (150g wheat flour, flour 10g Moringa leaves and soybean sprouts flour 40g) have modus 3 (like) and protein content 9g and iron content 3,21mg per serving size (100g cookies). Statistical analysis showed significant difference to the characteristic colour (p=0,000), odour (p=0,000), texture (p=0,000) and flavor (p=0,000).
DAYA TERIMA DAN TOTAL COST KOMBINASI SARI KULIT BUAH NAGA MERAH DAN SARI BUAH JAMBU BIJI MERAH Syafrina Mareta Sari; Annis Catur Adi; Dini Andrias Andrias
Media Gizi Indonesia Vol. 10 No. 2 (2015): JURNAL MEDIA GIZI INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.474 KB) | DOI: 10.20473/mgi.v10i2.128-135

Abstract

Antioxidant plays a role as the free radical savaging. The red guava and the red dragon fruit peel contain high  antioxidants so it is potentially to be a functional product. The aim of this study was to analyze the effect of red guava juice and red dragon fruit peel essence addition on yoghurt acceptability and total cost. The research used a factorial design of 5 repetitions on 5 formulas. There are 1 control formula (F0) and 4 modifi cation formulas (F1, F2, F4, F5) with the addition of red guava juice and red dragon fruit peel. The panelist on this research consist of 30 untrainedpanelists. Descriptive analysis and inferential statistic using Friedman and Wilcoxon Sign Rank Test with α = 0,05 were used to analyze the difference of acceptability between the formulas. Panelists showed that the best treatment was F4 yoghurt formula (44% addition of fruit juice with the ratio of guava juice and red dragon fruit peel 50:50) and it has the most preferred acceptability (color, aroma, texture, and taste) with the average value was 2.91 and food cost per serving (100 ml) was Rp 2.900,00. Formula F4 has a good acceptability and lower total cost than commercialyoghurt. This formula has a potential to be an alternative functional drink. The aroma of yoghurt can be increased by eliminate cooking process on the red dragon fruit peel juice.Keywords: acceptability, red guava, red dragon fruit peel, total cost, yoghurt
Co-Authors Abdurrahman Ali Adi Pranoto Adinda Nurul Istiqomah Adiningsih, Sri Aisyah Noer Auliyah Madani Pertiwi Al Himny Rusydy, Muhammad Al'aliyyu, Fikriyah Alfian Yusuf Ali Iqbal Tawakal Ali Iqbal Tawakal Ali Iqbal Tawakal Alristina, Arie Dwi Alzha Adila Harisina Amirah Adlia Amirah Adlia Andini Octaviana Putri Anggun Pinasti Ilahi Anna Smdyah Putri Anna Surgean Veterini Anna Surgean Veterini Anni Syntya Annisa Lutfiah Annisa Rizky Malichati Annisa Rizky Maulidiana Arina Farmalabitta Annis Arina Mufida Ersanti Arina Mufida Ersanti Arisya Humaira Ariza, Nahya Rahmatul Asrul Bahar Atsarina Anindya Aulanni'am, Aulanni'am Aulanni’am Aulanni’am Aulia Ramadhani Aulia, Noni Angelina Tazky Bambang Wahjuprajitno Bambang Wirdjadmaji Bambang Wirdjadmaji, Bambang Brilliant Anjar Saputro Cahya Ramadhanti Putri Calvin Ramadhani Chatarina Umbul Wahyuni Cindhy Pamela Kesuma Claudia Ni Luh Merry Marzeline Damar Rasti Adhika Daniel Christanto Dea Amanda Caressa Deandra Ardya Regitasari Deandra Ardya Regitasari Deta Oryza Isyana Dhiyah Ariba Oktaviani Dhiyah Ariba Oktaviani Diah Patria Nuringtyas Dian Puteri Andani Dini Andrias Andrias Dini Ririn Andrias Dominikus Raditya Atmaka Dyah Patria Nuringtyas Edinda Ayu Miranti Elya Sugianti Emyr Reisha Ishaura Endah Bardiati Enny Susanti Eny Qurniyawati Eva Flourentina Kusumawardani Eva Flourentina Kusumawardani Fajar Nuraida, Lutfi Faradillah Rahmy Savitri Faradita Meilinda Wulan Sari Farah Rosyihana Fadhila Farapti Farapti Fariani Syahrul Fathrizqita Aghnia Raudhany Fathrizqita Aghnia Raudhany Fauzatur Rofiqoh Febry Dian Permatasari Fifi Nirmala G Ghanynafi, Abdullah Hafizotun Tsaqifah Hafizotun Tsaqifah Hamzah Hamzah Hari Basuki Notobroto Haryana, Nila Reswari Hasanah, Dina Khomariyatul HENI RACHMAWATI Heni Rachmawati Hernita Riski Iga Ayuni Fatmala Iga Ema Dini Ikeu Ekayanti Ikhtiar, Ilham Irfa Ekasanti Irina Lailata Irwanto Irwanto Irwanto, Irwanto Isaura, Emyr Reisha Ivan Mahardika Yusuf Jayantini, Hikmah Ervina Junaida Astina Kamila Dwi Febrianti Kartini Kartini Lailatul Muniroh Lailia Wahyuliana Lathifah Ilmawati Laura Navika Yamani Lina Nurbaiti Lipu, Yanto Lisda Juniarsy Rahardjo Luki Mundiastuti Lyna Nur Afifah M. G. Bagus Ani Putra Mahmud Aditya Rifqi Mardiyono Yono Mei Lestari Ika Widyyati Meity Ardiana Melissa Ruslan Merryana Adriani Merryana Adriani Meyrina Putri Cahyariani Mohammad Fahmi Rasyidi Muamar Afdhal Mahendra Mufida Ersanti, Arina Mufidah Anisah Muhammad Atoillah Isfandiari Nabillah Eka Permatasari Nancy Margarita Rehatta Nanik Handayani Nelly Setiawaty Nisa, Hisma Zauharotun Novira Dian Rachmadia Nur Mufida Wulan Sari Nurlaila, Amilenia Nuthathai Sutthiwong Puspitasari, Prastiwi Novia Putra, M.G. Bagus Ani Putri Safrida Rahmawati Rachmad Suhanda Rachmahnia Pratiwi Rahmawati, Siti Irma Rakhmalia Imeldawati Rakhman, Dinis Puspita Ramadhan, Ramadivan Bagus Rasyidi, Mohammad Fahmi Ratna Candra Dewi Ratna Candra Dewi Rehatta, Nancy Margarita Reza Yuanita Ananta Riris Diana Rachmayanti Rita Ismawati Rita Ismawati Rizky Arifandy Amir Rizma Dwi Nastiti Romadhona, Salsabila S R Widya Areta Humaniora Justisia S.Pd. M Kes I Ketut Sudiana . Sakinah Aljuffrie Salisa, Wizara Salma Chalisha Santi Martini Sarmanu, Sarmanu Septa Katmawanti, Septa Septiana Purwandini Septyaningrum Putri Purwoto Shabhati, Berliandita Shafira Putri, Maurilla Shrimarti R Devi Sinaga, Niken Ayu Br Siti Nur Husnul Yusmiati Siti Rahayu Nadhiroh Siti Shofiya Novita Sari Siti Shofiya Novita Sari SOETJIPTO . Soetjipto Soetjipto SRI LESTARI Subijanto Marto Soedarmo Subijanto Marto Soedarmo Suryanto Suryanto Suryanto, Suryanto Syafrina Mareta Sari Tasya Shafa Salsabila Muda Tasya Shafa Salsabila Muda Thom, Fedora Ivena Timbuktu Harthana Tri Ratnaningsih Tri Ratnaningsih Tri Sulistijorini, Hinu Trias Mahmudiono Tsalatsa Aulia Mawaddah Ulfatul Karomah Vania Alvabella Wijaya Vetty Silvana Maulida Vidya Anggarini Rahmasari Vidya Anggarini Rahmasari Wening, Damar Maulidina Nur Widati Fatmaningrum Widijiati Widijiati Widjiati Widjiati Widodo Jatim P Widodo Jatim Pudjirahardjo Widyyati, Mei Lestari Ika Wizara Salisa Wizara Salisa Wizara Salisa Wizara Salisa Wizara Salisa Wizara Salisa Wizara Salisa Wizara Salisa Yama Dharma Putera Yasmin Muntaza Yeni Susanti Yulia Kurnia Sari Yusmiati, Siti Nur Husnul Yusuf, Alfian